AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003376

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003376.t1 MTR_4g076140 95.763 472 20 0 7 478 1 472 0.0 915
MS.gene003376.t1 MTR_5g009080 74.375 480 115 6 6 478 3 481 0.0 729
MS.gene003376.t1 MTR_3g022830 49.888 445 213 4 33 477 4 438 9.02e-161 462
MS.gene003376.t1 MTR_7g027190 33.778 450 216 6 43 475 289 673 4.55e-74 246
MS.gene003376.t1 MTR_5g097480 31.090 431 231 6 51 475 174 544 1.68e-65 220
MS.gene003376.t1 MTR_1g029420 29.837 429 235 8 53 475 224 592 4.93e-56 196
MS.gene003376.t1 MTR_1g106590 58.228 158 57 1 8 165 93 241 8.89e-56 189
MS.gene003376.t1 MTR_1g106590 69.318 88 26 1 291 378 253 339 2.27e-23 101
MS.gene003376.t1 MTR_3g089055 29.138 429 238 8 53 475 197 565 3.13e-54 191
MS.gene003376.t1 MTR_3g089055 29.138 429 238 8 53 475 202 570 3.71e-54 191
MS.gene003376.t1 MTR_3g056110 27.928 444 253 8 38 475 160 542 3.35e-52 185
MS.gene003376.t1 MTR_8g442410 29.955 444 230 12 47 475 148 525 5.41e-51 181
MS.gene003376.t1 MTR_4g064160 28.438 429 238 7 54 474 316 683 7.89e-50 181
MS.gene003376.t1 MTR_4g104020 28.798 441 238 11 48 475 143 520 6.30e-49 176
MS.gene003376.t1 MTR_4g064120 26.490 453 264 7 27 470 229 621 7.03e-47 172
MS.gene003376.t1 MTR_7g062120 27.103 428 247 5 54 475 372 740 1.71e-46 172
MS.gene003376.t1 MTR_4g064180 26.269 453 265 7 27 470 229 621 4.51e-45 167
MS.gene003376.t1 MTR_4g064150 27.174 460 261 11 26 474 336 732 5.30e-44 165
MS.gene003376.t1 MTR_2g097410 26.168 428 251 5 54 474 371 740 7.19e-44 164
MS.gene003376.t1 MTR_2g026250 27.570 428 243 10 55 475 229 596 1.25e-43 162
MS.gene003376.t1 MTR_3g022580 39.048 210 87 6 269 478 18 186 3.54e-43 151
MS.gene003376.t1 MTR_2g097467 26.376 436 235 8 54 474 289 653 6.13e-41 155
MS.gene003376.t1 MTR_2g097473 26.464 427 246 7 54 474 289 653 3.33e-40 153
MS.gene003376.t1 MTR_2g097350 27.002 437 254 9 43 474 263 639 2.06e-39 151
MS.gene003376.t1 MTR_4g102790 35.349 215 135 1 47 257 285 499 4.04e-38 147
MS.gene003376.t1 MTR_4g102790 31.373 153 98 5 328 474 510 661 3.14e-18 88.2
MS.gene003376.t1 MTR_2g097390 25.172 437 262 8 43 474 264 640 4.67e-38 147
MS.gene003376.t1 MTR_2g034280 29.306 447 235 15 45 475 194 575 5.96e-38 146
MS.gene003376.t1 MTR_1g096030 27.948 458 230 14 48 476 74 460 1.53e-37 143
MS.gene003376.t1 MTR_7g074650 40.314 191 111 2 58 247 442 630 3.82e-37 145
MS.gene003376.t1 MTR_7g074650 36.913 149 89 3 330 475 646 792 7.02e-20 93.6
MS.gene003376.t1 MTR_1g069725 27.689 437 239 12 46 477 101 465 6.07e-37 142
MS.gene003376.t1 MTR_2g097463 26.230 427 247 7 54 474 290 654 1.01e-36 143
MS.gene003376.t1 MTR_2g034250 27.938 451 239 14 45 475 201 585 1.03e-36 143
MS.gene003376.t1 MTR_3g065980 34.685 222 131 4 42 258 160 372 2.15e-34 135
MS.gene003376.t1 MTR_3g065980 35.758 165 93 2 324 475 376 540 4.14e-28 117
MS.gene003376.t1 MTR_2g034260 26.608 451 244 13 45 475 201 584 4.36e-33 132
MS.gene003376.t1 MTR_4g097080 24.658 438 249 10 53 475 132 503 4.02e-32 129
MS.gene003376.t1 MTR_2g089100 24.336 452 291 10 35 474 30 442 5.30e-32 127
MS.gene003376.t1 MTR_3g072710 25.708 459 250 14 41 474 107 499 1.17e-30 124
MS.gene003376.t1 MTR_3g022005 51.546 97 46 1 382 478 33 128 3.36e-30 114
MS.gene003376.t1 MTR_2g082090 33.333 225 140 3 51 269 209 429 5.83e-30 123
MS.gene003376.t1 MTR_2g082090 41.611 149 86 1 326 474 431 578 2.86e-29 121
MS.gene003376.t1 MTR_5g094450 31.556 225 145 3 38 254 146 369 7.18e-30 122
MS.gene003376.t1 MTR_5g094450 38.298 141 86 1 335 475 393 532 1.78e-25 109
MS.gene003376.t1 MTR_2g097310 28.352 261 171 5 29 281 251 503 2.76e-29 121
MS.gene003376.t1 MTR_4g064200 31.081 222 140 4 54 269 284 498 4.88e-29 120
MS.gene003376.t1 MTR_4g064200 32.000 150 101 1 326 474 500 649 4.97e-16 81.3
MS.gene003376.t1 MTR_7g109580 25.567 485 278 13 7 475 137 554 1.15e-28 119
MS.gene003376.t1 MTR_3g027430 44.915 118 44 1 137 254 184 280 1.68e-26 110
MS.gene003376.t1 MTR_4g095500 24.016 508 304 12 3 474 6 467 6.28e-26 110
MS.gene003376.t1 MTR_5g015950 24.536 485 283 14 25 474 9 445 1.31e-25 109
MS.gene003376.t1 MTR_3g053270 24.536 485 283 14 25 474 9 445 1.31e-25 109
MS.gene003376.t1 MTR_5g015490 32.110 218 138 4 48 256 108 324 5.12e-25 108
MS.gene003376.t1 MTR_5g058860 31.169 231 142 4 40 253 105 335 1.02e-24 107
MS.gene003376.t1 MTR_5g058860 26.667 150 107 3 327 474 348 496 4.65e-11 65.5
MS.gene003376.t1 MTR_4g133660 30.698 215 141 3 49 256 170 383 1.07e-23 104
MS.gene003376.t1 MTR_4g133660 34.228 149 97 1 326 474 395 542 1.88e-21 97.8
MS.gene003376.t1 MTR_2g097380 27.273 209 131 4 64 269 233 423 5.32e-23 102
MS.gene003376.t1 MTR_2g099110 31.481 216 136 5 45 250 65 278 1.77e-22 100
MS.gene003376.t1 MTR_1g086970 33.728 169 102 5 313 475 314 478 2.11e-21 97.1
MS.gene003376.t1 MTR_1g086970 25.000 248 165 8 45 272 97 343 1.57e-11 66.6
MS.gene003376.t1 MTR_4g076020 30.052 193 119 4 58 247 88 267 1.01e-20 94.7
MS.gene003376.t1 MTR_4g076020 39.597 149 84 4 329 475 285 429 1.30e-19 91.3
MS.gene003376.t1 MTR_4g077760 28.700 223 149 3 51 266 190 409 8.97e-20 92.4
MS.gene003376.t1 MTR_4g077760 30.055 183 115 6 301 475 378 555 1.83e-13 73.2
MS.gene003376.t1 MTR_3g021320 34.641 153 72 2 154 306 1 125 2.30e-19 84.3
MS.gene003376.t1 MTR_6g047750 26.331 357 191 11 130 475 367 662 3.31e-19 91.3
MS.gene003376.t1 MTR_7g057230 33.333 150 95 3 326 474 511 656 4.07e-19 90.9
MS.gene003376.t1 MTR_4g026485 25.059 427 242 11 55 475 270 624 4.71e-19 90.5
MS.gene003376.t1 MTR_0092s0100 32.941 170 106 3 25 194 352 513 3.66e-14 75.5
MS.gene003376.t1 MTR_0092s0100 32.941 170 106 3 25 194 349 510 3.85e-14 75.5
MS.gene003376.t1 MTR_8g093070 29.126 206 132 8 51 251 136 332 2.53e-13 72.4
MS.gene003376.t1 MTR_3g025340 45.902 61 33 0 112 172 9 69 6.17e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003376.t1 AT1G50420 62.733 483 169 5 5 478 2 482 0.0 611
MS.gene003376.t1 AT1G66350 32.037 437 221 8 42 475 143 506 4.51e-68 226
MS.gene003376.t1 AT5G48150 30.679 427 231 5 55 475 123 490 8.53e-66 220
MS.gene003376.t1 AT5G48150 30.679 427 231 5 55 475 123 490 8.53e-66 220
MS.gene003376.t1 AT5G48150 30.679 427 231 5 55 475 123 490 8.53e-66 220
MS.gene003376.t1 AT5G48150 30.679 427 231 5 55 475 123 490 8.53e-66 220
MS.gene003376.t1 AT2G04890 32.938 422 222 5 54 475 53 413 3.91e-65 216
MS.gene003376.t1 AT5G17490 30.464 453 231 11 37 475 134 516 7.69e-60 205
MS.gene003376.t1 AT1G50600 29.322 457 251 10 30 475 131 526 1.37e-58 202
MS.gene003376.t1 AT1G50600 29.386 456 250 10 31 475 207 601 4.14e-58 202
MS.gene003376.t1 AT1G50600 29.386 456 250 10 31 475 203 597 4.46e-58 202
MS.gene003376.t1 AT1G21450 30.769 429 232 7 53 475 224 593 4.28e-56 196
MS.gene003376.t1 AT1G14920 30.112 445 225 12 42 475 160 529 2.99e-53 187
MS.gene003376.t1 AT2G01570 29.888 445 226 13 42 475 212 581 1.04e-51 184
MS.gene003376.t1 AT5G41920 30.023 433 227 11 51 477 40 402 7.58e-46 165
MS.gene003376.t1 AT1G07530 27.955 440 227 10 54 474 396 764 4.43e-44 166
MS.gene003376.t1 AT3G54220 42.180 211 120 1 39 247 278 488 5.74e-41 155
MS.gene003376.t1 AT3G54220 35.616 146 92 2 332 477 509 652 7.96e-20 93.2
MS.gene003376.t1 AT2G29060 27.189 434 246 10 51 474 281 654 7.07e-40 152
MS.gene003376.t1 AT2G29060 27.189 434 246 10 51 474 318 691 9.97e-40 152
MS.gene003376.t1 AT1G07520 25.282 443 263 7 43 474 143 528 3.62e-39 149
MS.gene003376.t1 AT1G07520 25.282 443 263 7 43 474 143 528 3.62e-39 149
MS.gene003376.t1 AT5G66770 28.899 436 231 12 51 475 217 584 6.40e-39 149
MS.gene003376.t1 AT1G07520 25.282 443 263 7 43 474 308 693 1.78e-38 149
MS.gene003376.t1 AT1G07520 25.282 443 263 7 43 474 307 692 2.02e-38 149
MS.gene003376.t1 AT5G59450 25.806 434 254 7 51 475 224 598 1.48e-37 145
MS.gene003376.t1 AT2G29065 25.571 438 247 10 54 474 252 627 8.75e-37 143
MS.gene003376.t1 AT3G46600 25.926 432 254 6 53 478 3 374 2.78e-36 138
MS.gene003376.t1 AT2G37650 24.826 431 257 6 51 475 344 713 4.66e-36 142
MS.gene003376.t1 AT2G37650 24.826 431 257 6 51 475 332 701 4.84e-36 142
MS.gene003376.t1 AT3G46600 25.926 432 254 6 53 478 179 550 6.57e-35 137
MS.gene003376.t1 AT3G46600 25.843 445 263 7 40 478 199 582 1.60e-34 136
MS.gene003376.t1 AT1G63100 32.218 239 157 4 46 280 269 506 2.00e-31 128
MS.gene003376.t1 AT1G63100 33.125 160 94 2 328 474 494 653 1.38e-20 95.5
MS.gene003376.t1 AT1G63100 32.218 239 157 4 46 280 244 481 2.57e-31 127
MS.gene003376.t1 AT1G63100 33.125 160 94 2 328 474 469 628 9.96e-21 95.9
MS.gene003376.t1 AT3G03450 31.839 223 143 5 42 258 171 390 2.05e-30 124
MS.gene003376.t1 AT3G03450 38.854 157 94 2 320 475 390 545 1.08e-28 119
MS.gene003376.t1 AT5G52510 26.376 436 246 13 53 475 267 640 4.81e-30 124
MS.gene003376.t1 AT1G55580 38.255 149 91 1 328 475 297 445 4.36e-28 116
MS.gene003376.t1 AT1G55580 27.843 255 143 8 50 264 40 293 6.49e-13 70.9
MS.gene003376.t1 AT4G37650 32.389 247 137 7 27 256 131 364 7.44e-25 107
MS.gene003376.t1 AT3G50650 26.157 432 235 13 59 475 180 542 5.20e-24 105
MS.gene003376.t1 AT4G17230 34.000 150 98 1 326 475 377 525 6.96e-23 102
MS.gene003376.t1 AT4G17230 28.774 212 144 2 49 254 153 363 5.25e-22 99.4
MS.gene003376.t1 AT4G17230 34.000 150 98 1 326 475 377 525 6.96e-23 102
MS.gene003376.t1 AT4G17230 28.774 212 144 2 49 254 153 363 5.25e-22 99.4
MS.gene003376.t1 AT4G08250 30.337 267 154 10 1 253 68 316 1.23e-21 97.8
MS.gene003376.t1 AT3G49950 22.343 461 275 14 32 474 12 407 1.61e-16 82.0
MS.gene003376.t1 AT4G00150 27.179 195 133 5 286 477 368 556 8.15e-12 67.8

Find 110 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCATTCGAGAGATTT+AGG 0.125804 4.1:-28126619 MS.gene003376:CDS
CCGAATATGCTTCGGTTTAC+TGG 0.158109 4.1:+28126899 None:intergenic
AATGAGCCTCTAAGTTATTT+TGG 0.189632 4.1:-28126473 MS.gene003376:CDS
TTTCATCATCCCAGGCTAAA+AGG 0.243724 4.1:+28126987 None:intergenic
TGCAGCACTATTTGATTGTT+TGG 0.256288 4.1:-28126657 MS.gene003376:CDS
CAATCCTGTTGTCAGCAAAT+TGG 0.264169 4.1:-28127086 MS.gene003376:CDS
CATCATGGAGAAGAGCTTTA+AGG 0.269652 4.1:+28127696 None:intergenic
AGATTTGATTTAGCTGGATT+TGG 0.318965 4.1:-28126497 MS.gene003376:CDS
CAATATCAGCTTGGAGATCT+AGG 0.325494 4.1:-28126343 MS.gene003376:CDS
AATCTCTCGAATGAGTTTCT+TGG 0.330855 4.1:+28126623 None:intergenic
CCAGTAAACCGAAGCATATT+CGG 0.339215 4.1:-28126899 MS.gene003376:CDS
AGCATTTGTTCTCACAAATC+AGG 0.349043 4.1:-28127329 MS.gene003376:CDS
CTCCATGATGCCACCTTCAT+CGG 0.351823 4.1:-28127683 MS.gene003376:CDS
AACTTACCAAAATAACTTAG+AGG 0.353618 4.1:+28126467 None:intergenic
TTGAGTGCTTTGTGGATTCC+AGG 0.357455 4.1:+28127430 None:intergenic
AAACTCATTCGAGAGATTTA+GGG 0.358753 4.1:-28126618 MS.gene003376:CDS
ATGCCACCTTCATCGGGCTT+TGG 0.360289 4.1:-28127676 MS.gene003376:CDS
TGCTGACAACAGGATTGAAT+TGG 0.362322 4.1:+28127092 None:intergenic
CAATTTGCTTGAGAAAGATT+TGG 0.366215 4.1:-28126876 MS.gene003376:CDS
ATCTCCACACCCTTGGCTTA+AGG 0.366272 4.1:-28127653 MS.gene003376:CDS
TATAGAATGAAGGAGGAAAA+TGG 0.386592 4.1:-28126404 MS.gene003376:CDS
TGAACTTGAGTCAGGACTTA+TGG 0.391992 4.1:+28126841 None:intergenic
ATCTCTCGAATGAGTTTCTT+GGG 0.394420 4.1:+28126624 None:intergenic
CTCAAATGTGGAGGGCCATC+AGG 0.400333 4.1:+28127202 None:intergenic
GAGAAACTTCGTGTTAAAAC+CGG 0.400451 4.1:-28127049 MS.gene003376:CDS
CAGGATGAGCACTCAAAACT+TGG 0.403034 4.1:+28127221 None:intergenic
TTACCGAAGCACTTGCTGAT+AGG 0.405714 4.1:-28127468 MS.gene003376:CDS
CACGAAGTTTCTCAAAATCA+AGG 0.414533 4.1:+28127059 None:intergenic
GAGTAGAGTTGAGTGCTTTG+TGG 0.416744 4.1:+28127422 None:intergenic
TTTGAAGTGTTCAAAGAAAC+CGG 0.422703 4.1:+28126812 None:intergenic
TCCATGATGCCACCTTCATC+GGG 0.430671 4.1:-28127682 MS.gene003376:CDS
TTTATGAGATTTGATTTAGC+TGG 0.436460 4.1:-28126503 MS.gene003376:CDS
AGGCTAAAAGGGTATGCAAT+TGG 0.439239 4.1:+28126999 None:intergenic
AGGGTGGAGAAACTTCATTT+TGG 0.439547 4.1:-28126599 MS.gene003376:CDS
AAGAGGTTCATGCAGAGTTA+TGG 0.445441 4.1:-28126437 MS.gene003376:CDS
CGCTGCATAGTATCGCCGTC+AGG 0.446613 4.1:+28127505 None:intergenic
GGAACTGAAATCTGAGGAAA+GGG 0.447352 4.1:-28127632 MS.gene003376:CDS
GCTTTGGATCTCCACACCCT+TGG 0.447719 4.1:-28127660 MS.gene003376:CDS
CTTCATGGTTTCATCATCCC+AGG 0.449792 4.1:+28126979 None:intergenic
GAACAAGACTCTAATCATAA+TGG 0.450476 4.1:-28126722 MS.gene003376:CDS
TGCTAATATGCTGGCAAGAT+AGG 0.451606 4.1:-28126376 MS.gene003376:CDS
TCTGTTACAACCATCACCTT+TGG 0.451615 4.1:+28126743 None:intergenic
AAAGGAAAGGCATGAAAAGC+TGG 0.456379 4.1:-28126534 MS.gene003376:CDS
TTCATCATCCCAGGCTAAAA+GGG 0.472082 4.1:+28126988 None:intergenic
GCAAATTGCCGAATATGCTT+CGG 0.476281 4.1:+28126891 None:intergenic
CAAGATTACAAGCAGCTACA+TGG 0.487046 4.1:+28127572 None:intergenic
TTGCACAAGTGAGCAACAAA+TGG 0.493036 4.1:+28127596 None:intergenic
ATATGCTTCGGTTTACTGGA+TGG 0.504223 4.1:+28126903 None:intergenic
ACAAATGCTACTTTCATGAA+AGG 0.509715 4.1:+28127343 None:intergenic
AGGATACTTAAAGCATGGCC+TGG 0.511478 4.1:-28127448 MS.gene003376:CDS
TTGTAAGTGAATTCCATTCG+AGG 0.515092 4.1:+28126931 None:intergenic
CAAATGCTACTTTCATGAAA+GGG 0.516070 4.1:+28127344 None:intergenic
TCAGGCTATTATTGAAGCAA+TGG 0.520201 4.1:-28127311 MS.gene003376:CDS
TCCATGCTTGCTTCCCCTGA+CGG 0.525920 4.1:-28127520 MS.gene003376:CDS
CAAGAACATTATTGCATGTG+AGG 0.529680 4.1:-28126567 MS.gene003376:CDS
GCCGTCAGGGGAAGCAAGCA+TGG 0.529802 4.1:+28127519 None:intergenic
TCGAATGGAATTCACTTACA+AGG 0.530422 4.1:-28126929 MS.gene003376:CDS
AGAAACTTCGTGTTAAAACC+GGG 0.531168 4.1:-28127048 MS.gene003376:CDS
GGCATGAAAAGCTGGATAAG+TGG 0.531394 4.1:-28126526 MS.gene003376:CDS
GGTGATGATGAACTTGAGTC+AGG 0.534611 4.1:+28126833 None:intergenic
GCCCGATGAAGGTGGCATCA+TGG 0.534918 4.1:+28127681 None:intergenic
ATGTGAGGGATTAGAAAGAA+AGG 0.534942 4.1:-28126552 MS.gene003376:CDS
TATCCTATCAGCAAGTGCTT+CGG 0.537933 4.1:+28127465 None:intergenic
ATTTGATTGTTTGGAATCAA+CGG 0.538609 4.1:-28126648 MS.gene003376:CDS
ACTAATTGCTAGTGCCTCCC+CGG 0.543403 4.1:+28127030 None:intergenic
TGACTCAAGTTCATCATCAC+CGG 0.544363 4.1:-28126831 MS.gene003376:CDS
AGGAACTGAAATCTGAGGAA+AGG 0.544931 4.1:-28127633 MS.gene003376:CDS
GGAGATCCAAAGCCCGATGA+AGG 0.545996 4.1:+28127670 None:intergenic
GATTTCAGTTCCTTAAGCCA+AGG 0.546043 4.1:+28127643 None:intergenic
TTGTGAGGCATATAGAATGA+AGG 0.546355 4.1:-28126414 MS.gene003376:CDS
GTACAGCTATGTTGCAAGAA+GGG 0.546911 4.1:-28127741 MS.gene003376:CDS
CCAAGCTGATATTGAAAACA+AGG 0.551538 4.1:+28126352 None:intergenic
AGTTCCTTAAGCCAAGGGTG+TGG 0.551766 4.1:+28127649 None:intergenic
AGAAGTGAAATCCACTGAGC+AGG 0.552331 4.1:+28127244 None:intergenic
ATGGCTGTGTGCTAATATGC+TGG 0.553571 4.1:-28126385 MS.gene003376:CDS
CAATCAGTCTATGAGCTACC+TGG 0.553683 4.1:+28127143 None:intergenic
CCTCCACATTTGAGAATCAC+CGG 0.556070 4.1:-28127193 MS.gene003376:CDS
GAAGAAAGAGGTTCTCGACC+AGG 0.556759 4.1:-28127161 MS.gene003376:CDS
ATTTCAGTTCCTTAAGCCAA+GGG 0.557071 4.1:+28127644 None:intergenic
CTCTTTCTTCTGATGAACGC+CGG 0.562200 4.1:+28127174 None:intergenic
AGTGTAGAGCTTCTAGTAGC+CGG 0.564547 4.1:+28126684 None:intergenic
GCTATTATTGAAGCAATGGA+AGG 0.565201 4.1:-28127307 MS.gene003376:CDS
CTCATTCGAGAGATTTAGGG+TGG 0.565611 4.1:-28126615 MS.gene003376:CDS
AAGAACATTATTGCATGTGA+GGG 0.567712 4.1:-28126566 MS.gene003376:CDS
GGTACAGCTATGTTGCAAGA+AGG 0.572547 4.1:-28127742 MS.gene003376:CDS
GAGTTTATCACCAAAGGTGA+TGG 0.577759 4.1:-28126753 MS.gene003376:CDS
ACAACAGGATTGAATTGGAA+TGG 0.580239 4.1:+28127097 None:intergenic
CATGCAGAGTTATGGTTGTG+AGG 0.584714 4.1:-28126429 MS.gene003376:CDS
GCTGCATAGTATCGCCGTCA+GGG 0.585579 4.1:+28127506 None:intergenic
CAACTCTACTCGAGTGTCGA+TGG 0.592320 4.1:-28127410 MS.gene003376:CDS
CGGCGTTCATCAGAAGAAAG+AGG 0.593327 4.1:-28127173 MS.gene003376:CDS
TATTATTGAAGCAATGGAAG+GGG 0.594602 4.1:-28127305 MS.gene003376:CDS
TTGGTAAGTTGCAAGCAAAG+AGG 0.603635 4.1:-28126454 MS.gene003376:CDS
CTGATAGGATACTTAAAGCA+TGG 0.603739 4.1:-28127453 MS.gene003376:CDS
AGACATTTCTCAATGCATTG+TGG 0.605127 4.1:-28126775 MS.gene003376:CDS
GCTTAAGGAACTGAAATCTG+AGG 0.605648 4.1:-28127638 MS.gene003376:CDS
CTATTATTGAAGCAATGGAA+GGG 0.615371 4.1:-28127306 MS.gene003376:CDS
ATTGTGGAGTTTATCACCAA+AGG 0.618172 4.1:-28126759 MS.gene003376:CDS
GATCCAAAGCCCGATGAAGG+TGG 0.619417 4.1:+28127673 None:intergenic
CGGTGATTCTCAAATGTGGA+GGG 0.619559 4.1:+28127194 None:intergenic
TGAGGCATATAGAATGAAGG+AGG 0.622680 4.1:-28126411 MS.gene003376:CDS
AGGGATTAGAAAGAAAGGAA+AGG 0.622681 4.1:-28126547 MS.gene003376:CDS
ACGCCGGTGATTCTCAAATG+TGG 0.623446 4.1:+28127190 None:intergenic
CTGCATAGTATCGCCGTCAG+GGG 0.626726 4.1:+28127507 None:intergenic
CCGGTGATTCTCAAATGTGG+AGG 0.626889 4.1:+28127193 None:intergenic
ATGCTACTGAACCTGCTCAG+TGG 0.675660 4.1:-28127255 MS.gene003376:CDS
GAAACTTCGTGTTAAAACCG+GGG 0.688408 4.1:-28127047 MS.gene003376:CDS
GAACTGAAATCTGAGGAAAG+GGG 0.705778 4.1:-28127631 MS.gene003376:CDS
ACTTCGTGTTAAAACCGGGG+AGG 0.735326 4.1:-28127044 MS.gene003376:CDS
CTGACGGCGATACTATGCAG+CGG 0.754112 4.1:-28127504 MS.gene003376:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTTACCAAAATAACTTAG+AGG + chr4.1:28127624-28127643 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG - chr4.1:28127440-28127459 MS.gene003376:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG - chr4.1:28127585-28127604 MS.gene003376:CDS 25.0%
AAACTCATTCGAGAGATTTA+GGG - chr4.1:28127470-28127489 MS.gene003376:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG - chr4.1:28127522-28127541 MS.gene003376:CDS 30.0%
CTATTATTGAAGCAATGGAA+GGG - chr4.1:28126782-28126801 MS.gene003376:CDS 30.0%
GAACAAGACTCTAATCATAA+TGG - chr4.1:28127366-28127385 MS.gene003376:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG - chr4.1:28127684-28127703 MS.gene003376:CDS 30.0%
TATTATTGAAGCAATGGAAG+GGG - chr4.1:28126783-28126802 MS.gene003376:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG - chr4.1:28127615-28127634 MS.gene003376:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG - chr4.1:28127591-28127610 MS.gene003376:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG - chr4.1:28127212-28127231 MS.gene003376:CDS 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG - chr4.1:28127144-28127163 MS.gene003376:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG - chr4.1:28127380-28127399 MS.gene003376:CDS 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG + chr4.1:28127144-28127163 None:intergenic 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG + chr4.1:28127279-28127298 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG + chr4.1:28126748-28126767 None:intergenic 30.0%
!! CAAATGCTACTTTCATGAAA+GGG + chr4.1:28126747-28126766 None:intergenic 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG + chr4.1:28127127-28127146 None:intergenic 30.0%
AATCTCTCGAATGAGTTTCT+TGG + chr4.1:28127468-28127487 None:intergenic 35.0%
ACAACAGGATTGAATTGGAA+TGG + chr4.1:28126994-28127013 None:intergenic 35.0%
AGAAACTTCGTGTTAAAACC+GGG - chr4.1:28127040-28127059 MS.gene003376:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG - chr4.1:28127313-28127332 MS.gene003376:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG - chr4.1:28126759-28126778 MS.gene003376:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG - chr4.1:28127541-28127560 MS.gene003376:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG + chr4.1:28127467-28127486 None:intergenic 35.0%
ATGTGAGGGATTAGAAAGAA+AGG - chr4.1:28127536-28127555 MS.gene003376:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG - chr4.1:28127329-28127348 MS.gene003376:CDS 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG + chr4.1:28126447-28126466 None:intergenic 35.0%
CAAGAACATTATTGCATGTG+AGG - chr4.1:28127521-28127540 MS.gene003376:CDS 35.0%
CACGAAGTTTCTCAAAATCA+AGG + chr4.1:28127032-28127051 None:intergenic 35.0%
CCAAGCTGATATTGAAAACA+AGG + chr4.1:28127739-28127758 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG - chr4.1:28126635-28126654 MS.gene003376:CDS 35.0%
GAAACTCATTCGAGAGATTT+AGG - chr4.1:28127469-28127488 MS.gene003376:CDS 35.0%
GAGAAACTTCGTGTTAAAAC+CGG - chr4.1:28127039-28127058 MS.gene003376:CDS 35.0%
GCTATTATTGAAGCAATGGA+AGG - chr4.1:28126781-28126800 MS.gene003376:CDS 35.0%
TCAGGCTATTATTGAAGCAA+TGG - chr4.1:28126777-28126796 MS.gene003376:CDS 35.0%
TCGAATGGAATTCACTTACA+AGG - chr4.1:28127159-28127178 MS.gene003376:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG - chr4.1:28127431-28127450 MS.gene003376:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG + chr4.1:28127160-28127179 None:intergenic 35.0%
TTGTGAGGCATATAGAATGA+AGG - chr4.1:28127674-28127693 MS.gene003376:CDS 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG - chr4.1:28127736-28127755 MS.gene003376:CDS 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG + chr4.1:28127009-28127028 None:intergenic 35.0%
AAGAGGTTCATGCAGAGTTA+TGG - chr4.1:28127651-28127670 MS.gene003376:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG - chr4.1:28126455-28126474 MS.gene003376:CDS 40.0%
ATATGCTTCGGTTTACTGGA+TGG + chr4.1:28127188-28127207 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG + chr4.1:28126519-28126538 None:intergenic 40.0%
CAATATCAGCTTGGAGATCT+AGG - chr4.1:28127745-28127764 MS.gene003376:CDS 40.0%
CAATCCTGTTGTCAGCAAAT+TGG - chr4.1:28127002-28127021 MS.gene003376:CDS 40.0%
CATCATGGAGAAGAGCTTTA+AGG + chr4.1:28126395-28126414 None:intergenic 40.0%
CCAGTAAACCGAAGCATATT+CGG - chr4.1:28127189-28127208 MS.gene003376:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG - chr4.1:28127041-28127060 MS.gene003376:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG - chr4.1:28126457-28126476 MS.gene003376:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG - chr4.1:28127335-28127354 MS.gene003376:CDS 40.0%
GATTTCAGTTCCTTAAGCCA+AGG + chr4.1:28126448-28126467 None:intergenic 40.0%
GCAAATTGCCGAATATGCTT+CGG + chr4.1:28127200-28127219 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG - chr4.1:28126450-28126469 MS.gene003376:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG - chr4.1:28126456-28126475 MS.gene003376:CDS 40.0%
GTACAGCTATGTTGCAAGAA+GGG - chr4.1:28126347-28126366 MS.gene003376:CDS 40.0%
TATCCTATCAGCAAGTGCTT+CGG + chr4.1:28126626-28126645 None:intergenic 40.0%
TCTGTTACAACCATCACCTT+TGG + chr4.1:28127348-28127367 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG + chr4.1:28127250-28127269 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG - chr4.1:28127257-28127276 MS.gene003376:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG - chr4.1:28127677-28127696 MS.gene003376:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG - chr4.1:28127712-28127731 MS.gene003376:CDS 40.0%
TGCTGACAACAGGATTGAAT+TGG + chr4.1:28126999-28127018 None:intergenic 40.0%
TTCATCATCCCAGGCTAAAA+GGG + chr4.1:28127103-28127122 None:intergenic 40.0%
TTGCACAAGTGAGCAACAAA+TGG + chr4.1:28126495-28126514 None:intergenic 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG - chr4.1:28127634-28127653 MS.gene003376:CDS 40.0%
TTTCATCATCCCAGGCTAAA+AGG + chr4.1:28127104-28127123 None:intergenic 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG - chr4.1:28127554-28127573 MS.gene003376:CDS 40.0%
! AATTGCATACCCTTTTAGCC+TGG - chr4.1:28127091-28127110 MS.gene003376:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG + chr4.1:28127092-28127111 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG - chr4.1:28127489-28127508 MS.gene003376:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG - chr4.1:28127092-28127111 MS.gene003376:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG - chr4.1:28127494-28127513 MS.gene003376:CDS 40.0%
AGAAGTGAAATCCACTGAGC+AGG + chr4.1:28126847-28126866 None:intergenic 45.0%
AGGATACTTAAAGCATGGCC+TGG - chr4.1:28126640-28126659 MS.gene003376:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG + chr4.1:28127407-28127426 None:intergenic 45.0%
ATGGCTGTGTGCTAATATGC+TGG - chr4.1:28127703-28127722 MS.gene003376:CDS 45.0%
CAATCAGTCTATGAGCTACC+TGG + chr4.1:28126948-28126967 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG + chr4.1:28126870-28126889 None:intergenic 45.0%
CATGCAGAGTTATGGTTGTG+AGG - chr4.1:28127659-28127678 MS.gene003376:CDS 45.0%
CCGAATATGCTTCGGTTTAC+TGG + chr4.1:28127192-28127211 None:intergenic 45.0%
CCTCCACATTTGAGAATCAC+CGG - chr4.1:28126895-28126914 MS.gene003376:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG + chr4.1:28126897-28126916 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG - chr4.1:28127473-28127492 MS.gene003376:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG + chr4.1:28126917-28126936 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG + chr4.1:28127112-28127131 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG + chr4.1:28126669-28126688 None:intergenic 45.0%
GGCATGAAAAGCTGGATAAG+TGG - chr4.1:28127562-28127581 MS.gene003376:CDS 45.0%
GGTACAGCTATGTTGCAAGA+AGG - chr4.1:28126346-28126365 MS.gene003376:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG + chr4.1:28126661-28126680 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG - chr4.1:28127385-28127404 MS.gene003376:CDS 45.0%
! GGTGATGATGAACTTGAGTC+AGG + chr4.1:28127258-28127277 None:intergenic 45.0%
! TTACCGAAGCACTTGCTGAT+AGG - chr4.1:28126620-28126639 MS.gene003376:CDS 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG - chr4.1:28126871-28126890 MS.gene003376:CDS 45.0%
ACGCCGGTGATTCTCAAATG+TGG + chr4.1:28126901-28126920 None:intergenic 50.0%
ACTAATTGCTAGTGCCTCCC+CGG + chr4.1:28127061-28127080 None:intergenic 50.0%
ACTTCGTGTTAAAACCGGGG+AGG - chr4.1:28127044-28127063 MS.gene003376:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG + chr4.1:28126442-28126461 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG - chr4.1:28126435-28126454 MS.gene003376:CDS 50.0%
ATGCTACTGAACCTGCTCAG+TGG - chr4.1:28126833-28126852 MS.gene003376:CDS 50.0%
CCGGTGATTCTCAAATGTGG+AGG + chr4.1:28126898-28126917 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG - chr4.1:28126915-28126934 MS.gene003376:CDS 50.0%
CTCCATGATGCCACCTTCAT+CGG - chr4.1:28126405-28126424 MS.gene003376:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG - chr4.1:28126927-28126946 MS.gene003376:CDS 50.0%
TCCATGATGCCACCTTCATC+GGG - chr4.1:28126406-28126425 MS.gene003376:CDS 50.0%
!! CAACTCTACTCGAGTGTCGA+TGG - chr4.1:28126678-28126697 MS.gene003376:CDS 50.0%
ATGCCACCTTCATCGGGCTT+TGG - chr4.1:28126412-28126431 MS.gene003376:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG + chr4.1:28126889-28126908 None:intergenic 55.0%
CTGACGGCGATACTATGCAG+CGG - chr4.1:28126584-28126603 MS.gene003376:CDS 55.0%
CTGCATAGTATCGCCGTCAG+GGG + chr4.1:28126584-28126603 None:intergenic 55.0%
GATCCAAAGCCCGATGAAGG+TGG + chr4.1:28126418-28126437 None:intergenic 55.0%
GCTGCATAGTATCGCCGTCA+GGG + chr4.1:28126585-28126604 None:intergenic 55.0%
GGAGATCCAAAGCCCGATGA+AGG + chr4.1:28126421-28126440 None:intergenic 55.0%
TCCATGCTTGCTTCCCCTGA+CGG - chr4.1:28126568-28126587 MS.gene003376:CDS 55.0%
! GCTTTGGATCTCCACACCCT+TGG - chr4.1:28126428-28126447 MS.gene003376:CDS 55.0%
CGCTGCATAGTATCGCCGTC+AGG + chr4.1:28126586-28126605 None:intergenic 60.0%
GCCCGATGAAGGTGGCATCA+TGG + chr4.1:28126410-28126429 None:intergenic 60.0%
GCCGTCAGGGGAAGCAAGCA+TGG + chr4.1:28126572-28126591 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 28126337 28127773 28126337 ID=MS.gene003376
chr4.1 mRNA 28126337 28127773 28126337 ID=MS.gene003376.t1;Parent=MS.gene003376
chr4.1 exon 28126337 28127773 28126337 ID=MS.gene003376.t1.exon1;Parent=MS.gene003376.t1
chr4.1 CDS 28126337 28127773 28126337 ID=cds.MS.gene003376.t1;Parent=MS.gene003376.t1
Gene Sequence

>MS.gene003376

ATGTTACATGGTACAGCTATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCGGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAACCATGTAGCTGCTTGTAATCTTGAGCATGCAAATGCAACACTTGAGCAAATTTCCATGCTTGCTTCCCCTGACGGCGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCACTCAACTCTACTCGAGTGTCGATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATTATTGAAGCAATGGAAGGGGAGAGAATGATTCACATAATTGATCTCAATGCTACTGAACCTGCTCAGTGGATTTCACTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCCCTCCACATTTGAGAATCACCGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCATATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGTTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGGTCCCAAGAAACTCATTCGAGAGATTTAGGGTGGAGAAACTTCATTTTGGTGAGGAAATCAAGAACATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGAGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGCTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA
Protein sequence

>MS.gene003376.t1

MLHGTAMLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANATLEQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNATEPAQWISLLQVLSAHPDGPPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSIFGNLLEKDLVSSYTISPDSSSSSPVSLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTVPRNSFERFRVEKLHFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVLICWQDRSLFSISAWRSRK