AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003382

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003382.t1 MTR_4g076020 89.320 309 19 2 1 309 17 311 0.0 506
MS.gene003382.t1 MTR_7g074650 60.084 238 91 2 72 309 441 674 8.52e-95 298
MS.gene003382.t1 MTR_1g069725 51.417 247 112 2 63 308 103 342 1.37e-82 257
MS.gene003382.t1 MTR_7g027190 35.154 293 167 6 39 313 270 557 4.94e-48 171
MS.gene003382.t1 MTR_4g102790 36.719 256 155 4 63 312 286 540 8.15e-45 162
MS.gene003382.t1 MTR_3g065980 33.591 259 155 4 60 312 163 410 1.46e-38 143
MS.gene003382.t1 MTR_4g064150 29.927 274 180 3 51 312 343 616 4.20e-36 137
MS.gene003382.t1 MTR_5g058860 35.955 267 150 7 54 301 104 368 3.54e-34 130
MS.gene003382.t1 MTR_3g072710 36.559 279 152 9 53 308 104 380 6.28e-34 130
MS.gene003382.t1 MTR_4g104020 30.686 277 178 6 50 313 130 405 2.42e-33 128
MS.gene003382.t1 MTR_1g029420 35.271 258 149 7 69 313 225 477 2.73e-33 129
MS.gene003382.t1 MTR_2g097410 28.159 277 173 4 54 312 356 624 5.88e-33 129
MS.gene003382.t1 MTR_5g009080 32.524 206 136 2 60 265 47 249 1.27e-32 126
MS.gene003382.t1 MTR_4g064120 26.460 291 202 3 34 312 219 509 1.02e-31 125
MS.gene003382.t1 MTR_3g089055 34.109 258 152 7 69 313 198 450 3.44e-31 123
MS.gene003382.t1 MTR_4g064180 28.231 294 193 6 34 312 219 509 3.90e-31 123
MS.gene003382.t1 MTR_3g089055 35.039 254 146 8 69 308 203 451 3.91e-31 122
MS.gene003382.t1 MTR_5g094450 33.061 245 150 5 69 300 161 404 3.86e-30 119
MS.gene003382.t1 MTR_4g076140 33.810 210 128 3 60 265 39 241 5.39e-30 119
MS.gene003382.t1 MTR_4g077760 34.000 250 143 7 66 302 190 430 4.46e-29 117
MS.gene003382.t1 MTR_2g082090 32.061 262 160 6 51 300 196 451 6.26e-29 116
MS.gene003382.t1 MTR_8g442410 34.221 263 161 4 63 313 149 411 1.60e-28 115
MS.gene003382.t1 MTR_5g097480 33.197 244 147 5 69 300 177 416 1.90e-28 115
MS.gene003382.t1 MTR_4g097080 30.081 246 153 7 68 300 132 371 2.64e-28 114
MS.gene003382.t1 MTR_2g099110 30.864 243 151 7 69 300 74 310 3.11e-28 113
MS.gene003382.t1 MTR_1g096030 33.333 255 145 8 59 300 70 312 5.34e-28 113
MS.gene003382.t1 MTR_4g064200 29.057 265 167 6 61 312 276 532 8.05e-28 114
MS.gene003382.t1 MTR_3g056110 29.961 257 164 5 69 313 175 427 1.84e-27 112
MS.gene003382.t1 MTR_8g093070 32.812 256 151 7 53 301 125 366 2.25e-27 111
MS.gene003382.t1 MTR_5g015490 28.958 259 161 7 60 300 105 358 2.53e-27 111
MS.gene003382.t1 MTR_4g064160 27.737 274 182 5 51 312 298 567 5.55e-27 111
MS.gene003382.t1 MTR_2g097310 30.660 212 137 4 64 273 267 470 1.67e-26 110
MS.gene003382.t1 MTR_7g062120 31.336 217 133 5 64 273 367 574 2.24e-26 109
MS.gene003382.t1 MTR_2g097350 32.195 205 133 3 64 266 269 469 4.90e-26 108
MS.gene003382.t1 MTR_2g097467 28.195 266 164 6 63 312 283 537 5.76e-26 108
MS.gene003382.t1 MTR_2g097473 28.358 268 165 6 61 312 281 537 8.66e-26 107
MS.gene003382.t1 MTR_3g022830 31.122 196 132 2 70 265 26 218 1.70e-25 105
MS.gene003382.t1 MTR_2g097463 28.358 268 165 6 61 312 282 538 2.09e-25 106
MS.gene003382.t1 MTR_4g133660 31.200 250 155 6 64 300 170 415 3.82e-24 102
MS.gene003382.t1 MTR_2g097390 29.902 204 137 3 64 265 270 469 6.70e-24 102
MS.gene003382.t1 MTR_2g034280 29.213 267 177 3 59 313 193 459 2.16e-23 100
MS.gene003382.t1 MTR_2g034260 29.670 273 170 6 59 313 200 468 2.60e-23 100
MS.gene003382.t1 MTR_0092s0100 31.356 236 149 6 70 299 373 601 6.57e-23 99.4
MS.gene003382.t1 MTR_0092s0100 31.356 236 149 6 70 299 376 604 7.37e-23 99.4
MS.gene003382.t1 MTR_2g089100 24.460 278 175 5 69 312 52 328 5.12e-22 96.3
MS.gene003382.t1 MTR_4g026485 29.752 242 148 6 70 300 270 500 5.41e-22 96.7
MS.gene003382.t1 MTR_2g026250 28.458 253 158 7 64 300 223 468 1.22e-21 95.5
MS.gene003382.t1 MTR_2g034250 27.473 273 176 6 59 313 200 468 9.03e-21 93.2
MS.gene003382.t1 MTR_2g097380 28.378 222 135 5 54 273 209 408 1.14e-20 92.8
MS.gene003382.t1 MTR_7g109580 25.660 265 182 4 59 313 179 438 5.83e-19 87.4
MS.gene003382.t1 MTR_1g086970 22.388 268 194 6 59 313 96 362 5.59e-13 69.7
MS.gene003382.t1 MTR_8g077940 31.176 170 109 5 70 235 183 348 7.77e-13 69.3
MS.gene003382.t1 MTR_1g106590 30.000 120 82 1 63 182 124 241 1.18e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003382.t1 AT5G41920 68.846 260 78 2 53 309 27 286 5.76e-124 361
MS.gene003382.t1 AT3G54220 58.397 262 106 2 49 309 273 532 4.61e-97 300
MS.gene003382.t1 AT1G63100 35.827 254 157 4 64 312 247 499 7.12e-40 148
MS.gene003382.t1 AT1G63100 35.827 254 157 4 64 312 272 524 9.97e-40 147
MS.gene003382.t1 AT3G03450 34.615 260 158 5 60 312 174 428 1.16e-37 140
MS.gene003382.t1 AT1G66350 33.984 256 157 4 60 312 146 392 6.81e-35 132
MS.gene003382.t1 AT1G50420 35.437 206 132 1 60 265 48 252 1.28e-33 129
MS.gene003382.t1 AT5G17490 32.692 260 157 6 60 312 151 399 1.08e-32 127
MS.gene003382.t1 AT2G01570 30.651 261 164 5 60 312 215 466 1.34e-30 121
MS.gene003382.t1 AT1G14920 32.061 262 159 5 60 312 163 414 4.24e-30 119
MS.gene003382.t1 AT1G07520 27.306 271 177 5 59 312 144 411 1.51e-28 115
MS.gene003382.t1 AT1G07520 27.306 271 177 5 59 312 144 411 1.51e-28 115
MS.gene003382.t1 AT1G07520 27.306 271 177 5 59 312 309 576 2.01e-28 115
MS.gene003382.t1 AT1G07520 27.306 271 177 5 59 312 308 575 2.17e-28 115
MS.gene003382.t1 AT2G04890 33.333 243 149 6 64 300 50 285 4.47e-28 112
MS.gene003382.t1 AT1G07530 29.615 260 166 6 66 312 393 648 4.81e-28 114
MS.gene003382.t1 AT5G48150 31.687 243 151 5 70 300 123 362 7.34e-28 113
MS.gene003382.t1 AT5G48150 31.687 243 151 5 70 300 123 362 7.34e-28 113
MS.gene003382.t1 AT5G48150 31.687 243 151 5 70 300 123 362 7.34e-28 113
MS.gene003382.t1 AT5G48150 31.687 243 151 5 70 300 123 362 7.34e-28 113
MS.gene003382.t1 AT4G37650 29.183 257 157 7 69 305 146 397 9.60e-28 113
MS.gene003382.t1 AT5G59450 27.636 275 180 5 52 312 210 479 1.19e-27 113
MS.gene003382.t1 AT5G66770 33.852 257 151 8 66 312 217 464 1.37e-27 112
MS.gene003382.t1 AT1G50600 33.198 247 149 5 66 300 156 398 3.00e-27 111
MS.gene003382.t1 AT1G21450 31.008 258 160 6 69 313 225 477 3.22e-27 112
MS.gene003382.t1 AT1G50600 33.198 247 149 5 66 300 227 469 5.11e-27 111
MS.gene003382.t1 AT1G50600 33.198 247 149 5 66 300 231 473 5.79e-27 111
MS.gene003382.t1 AT3G50650 34.322 236 135 6 87 312 193 418 6.35e-27 110
MS.gene003382.t1 AT2G29060 33.679 193 115 4 66 249 281 469 1.07e-25 107
MS.gene003382.t1 AT2G29060 33.679 193 115 4 66 249 318 506 1.31e-25 107
MS.gene003382.t1 AT4G17230 32.061 262 162 5 64 313 153 410 2.53e-25 106
MS.gene003382.t1 AT4G17230 32.061 262 162 5 64 313 153 410 2.53e-25 106
MS.gene003382.t1 AT2G37650 28.352 261 169 6 64 312 330 584 2.68e-25 106
MS.gene003382.t1 AT2G37650 28.352 261 169 6 64 312 342 596 3.03e-25 106
MS.gene003382.t1 AT3G46600 28.185 259 169 4 66 312 1 254 3.64e-25 104
MS.gene003382.t1 AT3G46600 27.969 261 171 4 64 312 207 462 4.63e-25 105
MS.gene003382.t1 AT3G46600 28.185 259 169 4 66 312 177 430 9.40e-25 104
MS.gene003382.t1 AT2G29065 28.462 260 169 5 69 312 252 510 2.47e-24 103
MS.gene003382.t1 AT4G08250 29.323 266 170 8 55 306 93 354 1.97e-23 100
MS.gene003382.t1 AT1G55580 32.624 282 147 11 69 308 44 324 3.31e-22 96.7
MS.gene003382.t1 AT3G49950 27.306 271 164 10 69 312 30 294 3.39e-16 79.0
MS.gene003382.t1 AT4G00150 29.707 239 132 10 70 295 209 424 5.21e-16 79.0
MS.gene003382.t1 AT3G60630 30.952 168 99 5 145 299 329 492 6.32e-14 72.8
MS.gene003382.t1 AT4G36710 27.962 211 135 7 40 238 90 295 3.38e-11 64.3

Find 71 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACGGTCGTGATCAATTT+TGG 0.239304 4.1:-28236462 None:intergenic
TCTACAGGTCTCAAACTTCT+AGG 0.268544 4.1:+28235772 MS.gene003382:CDS
GTTACCGATTTAGCAGTTAA+TGG 0.272210 4.1:-28235973 None:intergenic
GATATAACAGGAAGTGATTT+AGG 0.289920 4.1:+28236411 MS.gene003382:CDS
CATCCGATTGAGGGTAAAAT+TGG 0.305802 4.1:+28236309 MS.gene003382:CDS
TGATATCAAGATCAATGATA+TGG 0.341996 4.1:-28236122 None:intergenic
ATCCGCTCGGTGAGAATTAC+CGG 0.347135 4.1:+28236201 MS.gene003382:CDS
CATTGTTTGTATGATATAAC+AGG 0.349848 4.1:+28236399 MS.gene003382:CDS
AGATTCTTCTCCTTCGGTTT+CGG 0.352465 4.1:-28235752 None:intergenic
ACCTGTAGATTCTTCTCCTT+CGG 0.364116 4.1:-28235758 None:intergenic
AATTGGAAGTGTAACTGAAC+CGG 0.391845 4.1:+28236326 MS.gene003382:CDS
CTCGCCATTAACTGCTAAAT+CGG 0.397282 4.1:+28235969 MS.gene003382:CDS
GTTCAGTTATCTCCGGTAAA+AGG 0.402006 4.1:-28235852 None:intergenic
ACTGAACTCTCTTCACCGTT+TGG 0.403561 4.1:+28235868 MS.gene003382:CDS
CCCGAGCGAACTCGCAAAAT+CGG 0.412376 4.1:-28236271 None:intergenic
ATTGATCACGACCGTTGAAC+AGG 0.422992 4.1:+28236467 MS.gene003382:CDS
ATGAGGCAATCGTGGTTCAT+TGG 0.429274 4.1:+28236370 MS.gene003382:CDS
TCCGAGCTGTTAGAATCAAC+CGG 0.448871 4.1:+28236237 MS.gene003382:CDS
TTCGAGTTTCATCCGATTGA+GGG 0.456448 4.1:+28236300 MS.gene003382:CDS
GTGTTCGCGTTGAGCGCGTT+TGG 0.458235 4.1:-28235599 None:intergenic
TCGGTGAGAATTACCGGTTT+CGG 0.471801 4.1:+28236207 MS.gene003382:CDS
TGAACCACGATTGCCTCATT+CGG 0.484162 4.1:-28236366 None:intergenic
CAACGGTGAAACCGAGTTGT+AGG 0.484579 4.1:-28236034 None:intergenic
TGTTCCACATCCTTGCTTCG+AGG 0.493152 4.1:+28236169 MS.gene003382:CDS
GCAAGGATGTGGAACAATCC+TGG 0.495564 4.1:-28236162 None:intergenic
ACAAGTCTTTGAACATGAAA+CGG 0.502255 4.1:+28235726 MS.gene003382:CDS
AAAATCGGCGAGTCTTCTTC+CGG 0.502799 4.1:-28236256 None:intergenic
GTTCGAGTTTCATCCGATTG+AGG 0.506283 4.1:+28236299 MS.gene003382:CDS
TTCACCGTTTGGAACTTCAC+CGG 0.509521 4.1:+28235879 MS.gene003382:CDS
ATATCATGCAAGGACTTCAA+TGG 0.519599 4.1:+28236139 MS.gene003382:CDS
TCGACCTCGAAGCAAGGATG+TGG 0.523785 4.1:-28236173 None:intergenic
CTCGGAAGAAGATCCGAAAC+CGG 0.524772 4.1:-28236220 None:intergenic
TCCGGTTGATTCTAACAGCT+CGG 0.529075 4.1:-28236238 None:intergenic
GGTCTAACACCGAGTTGACC+CGG 0.536418 4.1:-28236345 None:intergenic
ATGCAAGGACTTCAATGGCC+AGG 0.537402 4.1:+28236144 MS.gene003382:CDS
CTACAACTCGGTTTCACCGT+TGG 0.539952 4.1:+28236035 MS.gene003382:CDS
CTACAGGTCTCAAACTTCTA+GGG 0.540758 4.1:+28235773 MS.gene003382:CDS
TCTTCTTCGACCTCGAAGCA+AGG 0.546617 4.1:-28236179 None:intergenic
CGCGTTTCAATCCTACAACT+CGG 0.552518 4.1:+28236023 MS.gene003382:CDS
ACTCGGTGTTAGACCGAATG+AGG 0.563043 4.1:+28236353 MS.gene003382:CDS
GAAGAGAGTTCAGTTATCTC+CGG 0.569254 4.1:-28235859 None:intergenic
AAATACGCACCAACTCGCTC+CGG 0.573807 4.1:-28235898 None:intergenic
ACCGAAGGAGAAGAATCTAC+AGG 0.582225 4.1:+28235757 MS.gene003382:CDS
AAAGGTCGTTGGCGAAGTCG+AGG 0.582557 4.1:-28235834 None:intergenic
ATTGATCTTGATATCATGCA+AGG 0.582635 4.1:+28236129 MS.gene003382:CDS
ATTGGAAGTGTAACTGAACC+GGG 0.584733 4.1:+28236327 MS.gene003382:CDS
CGCTCCGGTGAAGTTCCAAA+CGG 0.584887 4.1:-28235883 None:intergenic
GAACAGGATCTTAGCCATGC+TGG 0.589797 4.1:+28236483 MS.gene003382:CDS
ATGTGCTGAGTGTGTTGCCA+TGG 0.593544 4.1:+28235807 MS.gene003382:CDS
GGAACTTCACCGGAGCGAGT+TGG 0.594130 4.1:+28235889 MS.gene003382:CDS
GGCGAAGTCGAGGTTATCCA+TGG 0.595794 4.1:-28235824 None:intergenic
GAAACGGAAACCGAAACCGA+AGG 0.606138 4.1:+28235742 MS.gene003382:CDS
ATGCTGGTAGCTTCTTAGCG+AGG 0.608930 4.1:+28236499 MS.gene003382:CDS
CGTGGTGGAATACGACGCAC+AGG 0.613548 4.1:+28235687 MS.gene003382:CDS
TATCTCCGGTAAAAGGTCGT+TGG 0.618446 4.1:-28235845 None:intergenic
GAAAGATGGTTCATGAACGT+TGG 0.619264 4.1:-28235635 None:intergenic
ACATAAGCAATTAAATCAGA+CGG 0.619937 4.1:-28236873 None:intergenic
CGTGTGGTGAGTTCTTGTCT+CGG 0.629592 4.1:+28235940 MS.gene003382:CDS
GTAACTGAACCGGGTCAACT+CGG 0.642976 4.1:+28236336 MS.gene003382:CDS
TGAGATTGCTGACTCAGCTG+AGG 0.650293 4.1:+28236439 MS.gene003382:CDS
ACAAGCGTTACAAGCGCGTG+TGG 0.650613 4.1:+28235924 MS.gene003382:CDS
CTGTGCGTCGTATTCCACCA+CGG 0.661662 4.1:-28235686 None:intergenic
CTCTAGTGAGAAAACCGTGG+TGG 0.670910 4.1:+28235672 MS.gene003382:CDS
ATGGCTAAGATCCTGTTCAA+CGG 0.673618 4.1:-28236478 None:intergenic
TCGCTAAGAAGCTACCAGCA+TGG 0.677504 4.1:-28236497 None:intergenic
TAGACCGAATGAGGCAATCG+TGG 0.677791 4.1:+28236362 MS.gene003382:CDS
AAGTGTGAGAATTTCACCAA+CGG 0.681780 4.1:-28236051 None:intergenic
GAGGTCGAAGAAGATCCGCT+CGG 0.688733 4.1:+28236188 MS.gene003382:CDS
AACCGGTAATTCTCACCGAG+CGG 0.697234 4.1:-28236203 None:intergenic
CTACTCTAGTGAGAAAACCG+TGG 0.700727 4.1:+28235669 MS.gene003382:CDS
TCAATCGGATGAAACTCGAA+CGG 0.709737 4.1:-28236297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CATTGTTTGTATGATATAAC+AGG + chr4.1:28236399-28236418 MS.gene003382:CDS 25.0%
! TGATATCAAGATCAATGATA+TGG - chr4.1:28236125-28236144 None:intergenic 25.0%
ACAAGTCTTTGAACATGAAA+CGG + chr4.1:28235726-28235745 MS.gene003382:CDS 30.0%
ATTGATCTTGATATCATGCA+AGG + chr4.1:28236129-28236148 MS.gene003382:CDS 30.0%
GATATAACAGGAAGTGATTT+AGG + chr4.1:28236411-28236430 MS.gene003382:CDS 30.0%
!! TAGTTAGGTCTTTTGAAAGA+TGG - chr4.1:28235652-28235671 None:intergenic 30.0%
AAGTGTGAGAATTTCACCAA+CGG - chr4.1:28236054-28236073 None:intergenic 35.0%
ATAAGGAACATTATCGCTGT+TGG + chr4.1:28236633-28236652 MS.gene003382:intron 35.0%
ATATCATGCAAGGACTTCAA+TGG + chr4.1:28236139-28236158 MS.gene003382:CDS 35.0%
ATTTCGACCCGTTTCATTAA+GGG + chr4.1:28236719-28236738 MS.gene003382:intron 35.0%
TATACATTGGTGGAAGAGAA+TGG + chr4.1:28236795-28236814 MS.gene003382:intron 35.0%
! AATTGGAAGTGTAACTGAAC+CGG + chr4.1:28236326-28236345 MS.gene003382:CDS 35.0%
! CTTCCAATTTTACCCTCAAT+CGG - chr4.1:28236315-28236334 None:intergenic 35.0%
! GTTTTCTCACTAGAGTAGTT+AGG - chr4.1:28235667-28235686 None:intergenic 35.0%
! TGCGAATCAAGCAATTTTTC+AGG + chr4.1:28236077-28236096 MS.gene003382:CDS 35.0%
!! GTTACCGATTTAGCAGTTAA+TGG - chr4.1:28235976-28235995 None:intergenic 35.0%
!!! TCAAGCAATTTTTCAGGCTT+TGG + chr4.1:28236083-28236102 MS.gene003382:CDS 35.0%
ACCAATGTATATCCTCTCCA+AGG - chr4.1:28236786-28236805 None:intergenic 40.0%
ACCTGTAGATTCTTCTCCTT+CGG - chr4.1:28235761-28235780 None:intergenic 40.0%
AGATTCTTCTCCTTCGGTTT+CGG - chr4.1:28235755-28235774 None:intergenic 40.0%
ATGGCTAAGATCCTGTTCAA+CGG - chr4.1:28236481-28236500 None:intergenic 40.0%
CATCCGATTGAGGGTAAAAT+TGG + chr4.1:28236309-28236328 MS.gene003382:CDS 40.0%
CTACAGGTCTCAAACTTCTA+GGG + chr4.1:28235773-28235792 MS.gene003382:CDS 40.0%
CTCGCCATTAACTGCTAAAT+CGG + chr4.1:28235969-28235988 MS.gene003382:CDS 40.0%
GAAAGATGGTTCATGAACGT+TGG - chr4.1:28235638-28235657 None:intergenic 40.0%
GAAGAGAGTTCAGTTATCTC+CGG - chr4.1:28235862-28235881 None:intergenic 40.0%
GATTTCGACCCGTTTCATTA+AGG + chr4.1:28236718-28236737 MS.gene003382:intron 40.0%
GTTCAGTTATCTCCGGTAAA+AGG - chr4.1:28235855-28235874 None:intergenic 40.0%
TAATGAAACGGGTCGAAATC+CGG - chr4.1:28236718-28236737 None:intergenic 40.0%
TCAATCGGATGAAACTCGAA+CGG - chr4.1:28236300-28236319 None:intergenic 40.0%
TCCTTGGAGAGGATATACAT+TGG + chr4.1:28236782-28236801 MS.gene003382:intron 40.0%
TCTACAGGTCTCAAACTTCT+AGG + chr4.1:28235772-28235791 MS.gene003382:CDS 40.0%
TGAAGTGAAAGTTGAGAGGT+GGG + chr4.1:28236674-28236693 MS.gene003382:intron 40.0%
TTGGAGAGGATATACATTGG+TGG + chr4.1:28236785-28236804 MS.gene003382:intron 40.0%
TTTCGACCCGTTTCATTAAG+GGG + chr4.1:28236720-28236739 MS.gene003382:intron 40.0%
! ATTGGAAGTGTAACTGAACC+GGG + chr4.1:28236327-28236346 MS.gene003382:CDS 40.0%
! CTTTGTTGATTGCTTCTGCT+TGG + chr4.1:28236847-28236866 MS.gene003382:intron 40.0%
! TCAACGGTCGTGATCAATTT+TGG - chr4.1:28236465-28236484 None:intergenic 40.0%
! TTCGAGTTTCATCCGATTGA+GGG + chr4.1:28236300-28236319 MS.gene003382:CDS 40.0%
!!! GCAATTTTTCAGGCTTTGGA+TGG + chr4.1:28236087-28236106 MS.gene003382:CDS 40.0%
AAAATCGGCGAGTCTTCTTC+CGG - chr4.1:28236259-28236278 None:intergenic 45.0%
AAGTGAAAGTTGAGAGGTGG+GGG + chr4.1:28236676-28236695 MS.gene003382:intron 45.0%
ACCGAAGGAGAAGAATCTAC+AGG + chr4.1:28235757-28235776 MS.gene003382:CDS 45.0%
ACTGAACTCTCTTCACCGTT+TGG + chr4.1:28235868-28235887 MS.gene003382:CDS 45.0%
AGGAACATTATCGCTGTTGG+TGG + chr4.1:28236636-28236655 MS.gene003382:intron 45.0%
ATGAGGCAATCGTGGTTCAT+TGG + chr4.1:28236370-28236389 MS.gene003382:CDS 45.0%
ATGGTTCTCTCAAGCTTGGT+TGG + chr4.1:28236814-28236833 MS.gene003382:intron 45.0%
ATTGATCACGACCGTTGAAC+AGG + chr4.1:28236467-28236486 MS.gene003382:CDS 45.0%
CGCGTTTCAATCCTACAACT+CGG + chr4.1:28236023-28236042 MS.gene003382:CDS 45.0%
CGGGTTACCCCTTAATGAAA+CGG - chr4.1:28236730-28236749 None:intergenic 45.0%
CGTTTCATTAAGGGGTAACC+CGG + chr4.1:28236728-28236747 MS.gene003382:intron 45.0%
CTACTCTAGTGAGAAAACCG+TGG + chr4.1:28235669-28235688 MS.gene003382:CDS 45.0%
CTGGTGAAGTGAAAGTTGAG+AGG + chr4.1:28236670-28236689 MS.gene003382:intron 45.0%
GAAGTGAAAGTTGAGAGGTG+GGG + chr4.1:28236675-28236694 MS.gene003382:intron 45.0%
GAGAATGGTTCTCTCAAGCT+TGG + chr4.1:28236810-28236829 MS.gene003382:intron 45.0%
GGAACATTATCGCTGTTGGT+GGG + chr4.1:28236637-28236656 MS.gene003382:intron 45.0%
GGGTTACCCCTTAATGAAAC+GGG - chr4.1:28236729-28236748 None:intergenic 45.0%
GTGAAGTGAAAGTTGAGAGG+TGG + chr4.1:28236673-28236692 MS.gene003382:intron 45.0%
TAGCTTCTTAGCGAGGTTTG+TGG + chr4.1:28236506-28236525 MS.gene003382:intron 45.0%
TATCTCCGGTAAAAGGTCGT+TGG - chr4.1:28235848-28235867 None:intergenic 45.0%
TCCGAGCTGTTAGAATCAAC+CGG + chr4.1:28236237-28236256 MS.gene003382:CDS 45.0%
TGAACCACGATTGCCTCATT+CGG - chr4.1:28236369-28236388 None:intergenic 45.0%
TGTTGTTGGGGATGTTTCCT+TGG + chr4.1:28236766-28236785 MS.gene003382:intron 45.0%
TTCACTTCACCAGTCCTCTT+AGG - chr4.1:28236663-28236682 None:intergenic 45.0%
TTCTCTCAAGCTTGGTTGGA+AGG + chr4.1:28236818-28236837 MS.gene003382:intron 45.0%
! GGATAGTGTTGAGAGGCATA+TGG + chr4.1:28236584-28236603 MS.gene003382:intron 45.0%
! GTTCGAGTTTCATCCGATTG+AGG + chr4.1:28236299-28236318 MS.gene003382:CDS 45.0%
! TAGTGTTGAGAGGCATATGG+TGG + chr4.1:28236587-28236606 MS.gene003382:intron 45.0%
! TCCGGTTGATTCTAACAGCT+CGG - chr4.1:28236241-28236260 None:intergenic 45.0%
! TTCACCGTTTGGAACTTCAC+CGG + chr4.1:28235879-28235898 MS.gene003382:CDS 45.0%
!! TCGGTGAGAATTACCGGTTT+CGG + chr4.1:28236207-28236226 MS.gene003382:CDS 45.0%
!! TCTCAGGCGAGTTTGTTGTT+GGG + chr4.1:28236753-28236772 MS.gene003382:intron 45.0%
!! TTCTCAGGCGAGTTTGTTGT+TGG + chr4.1:28236752-28236771 MS.gene003382:intron 45.0%
AAATACGCACCAACTCGCTC+CGG - chr4.1:28235901-28235920 None:intergenic 50.0%
AACAAACTCGCCTGAGAAGC+CGG - chr4.1:28236750-28236769 None:intergenic 50.0%
AACCGGTAATTCTCACCGAG+CGG - chr4.1:28236206-28236225 None:intergenic 50.0%
AGCTGTTGGGGTGTGAGATA+AGG + chr4.1:28236616-28236635 MS.gene003382:intron 50.0%
AGTGAAAGTTGAGAGGTGGG+GGG + chr4.1:28236677-28236696 MS.gene003382:intron 50.0%
ATGCAAGGACTTCAATGGCC+AGG + chr4.1:28236144-28236163 MS.gene003382:CDS 50.0%
ATGCTGGTAGCTTCTTAGCG+AGG + chr4.1:28236499-28236518 MS.gene003382:CDS 50.0%
ATGTGCTGAGTGTGTTGCCA+TGG + chr4.1:28235807-28235826 MS.gene003382:CDS 50.0%
CAACGGTGAAACCGAGTTGT+AGG - chr4.1:28236037-28236056 None:intergenic 50.0%
CGTGTGGTGAGTTCTTGTCT+CGG + chr4.1:28235940-28235959 MS.gene003382:CDS 50.0%
CTACAACTCGGTTTCACCGT+TGG + chr4.1:28236035-28236054 MS.gene003382:CDS 50.0%
CTCGGAAGAAGATCCGAAAC+CGG - chr4.1:28236223-28236242 None:intergenic 50.0%
CTCTAGTGAGAAAACCGTGG+TGG + chr4.1:28235672-28235691 MS.gene003382:CDS 50.0%
CTTCTTAGCGAGGTTTGTGG+AGG + chr4.1:28236509-28236528 MS.gene003382:intron 50.0%
GAAACGGAAACCGAAACCGA+AGG + chr4.1:28235742-28235761 MS.gene003382:CDS 50.0%
GAACAGGATCTTAGCCATGC+TGG + chr4.1:28236483-28236502 MS.gene003382:CDS 50.0%
GCAAGGATGTGGAACAATCC+TGG - chr4.1:28236165-28236184 None:intergenic 50.0%
GTAACTGAACCGGGTCAACT+CGG + chr4.1:28236336-28236355 MS.gene003382:CDS 50.0%
TAGACCGAATGAGGCAATCG+TGG + chr4.1:28236362-28236381 MS.gene003382:CDS 50.0%
TCGCTAAGAAGCTACCAGCA+TGG - chr4.1:28236500-28236519 None:intergenic 50.0%
TCTTCTTCGACCTCGAAGCA+AGG - chr4.1:28236182-28236201 None:intergenic 50.0%
TGTTCCACATCCTTGCTTCG+AGG + chr4.1:28236169-28236188 MS.gene003382:CDS 50.0%
TTGGGGATGTTTCCTTGGAG+AGG + chr4.1:28236771-28236790 MS.gene003382:intron 50.0%
! ATCCGCTCGGTGAGAATTAC+CGG + chr4.1:28236201-28236220 MS.gene003382:CDS 50.0%
! CTTCGCCAACGACCTTTTAC+CGG + chr4.1:28235840-28235859 MS.gene003382:CDS 50.0%
! TGGGTGTGGATAGTGTTGAG+AGG + chr4.1:28236577-28236596 MS.gene003382:intron 50.0%
!! ACTCGGTGTTAGACCGAATG+AGG + chr4.1:28236353-28236372 MS.gene003382:CDS 50.0%
!! CTCAGGCGAGTTTGTTGTTG+GGG + chr4.1:28236754-28236773 MS.gene003382:intron 50.0%
!! TGAGATTGCTGACTCAGCTG+AGG + chr4.1:28236439-28236458 MS.gene003382:CDS 50.0%
AAAGGTCGTTGGCGAAGTCG+AGG - chr4.1:28235837-28235856 None:intergenic 55.0%
ACAAACTCGCCTGAGAAGCC+GGG - chr4.1:28236749-28236768 None:intergenic 55.0%
ACAAGCGTTACAAGCGCGTG+TGG + chr4.1:28235924-28235943 MS.gene003382:CDS 55.0%
CCCGAGCGAACTCGCAAAAT+CGG - chr4.1:28236274-28236293 None:intergenic 55.0%
CGCTCCGGTGAAGTTCCAAA+CGG - chr4.1:28235886-28235905 None:intergenic 55.0%
CTGTGCGTCGTATTCCACCA+CGG - chr4.1:28235689-28235708 None:intergenic 55.0%
GAGGTCGAAGAAGATCCGCT+CGG + chr4.1:28236188-28236207 MS.gene003382:CDS 55.0%
GGCGAAGTCGAGGTTATCCA+TGG - chr4.1:28235827-28235846 None:intergenic 55.0%
TCGACCTCGAAGCAAGGATG+TGG - chr4.1:28236176-28236195 None:intergenic 55.0%
! ATGGTGGAGCAGCAGCTGTT+GGG + chr4.1:28236603-28236622 MS.gene003382:intron 55.0%
! CCGATTTTGCGAGTTCGCTC+GGG + chr4.1:28236271-28236290 MS.gene003382:CDS 55.0%
! GGTCTAACACCGAGTTGACC+CGG - chr4.1:28236348-28236367 None:intergenic 55.0%
! GGTGGGGGGATGAGTTGAAA+CGG + chr4.1:28236691-28236710 MS.gene003382:intron 55.0%
! GTGGGGGGATGAGTTGAAAC+GGG + chr4.1:28236692-28236711 MS.gene003382:intron 55.0%
! TATGGTGGAGCAGCAGCTGT+TGG + chr4.1:28236602-28236621 MS.gene003382:intron 55.0%
! TCTAGGGGATGGTTTGGGTG+TGG + chr4.1:28236563-28236582 MS.gene003382:intron 55.0%
!! GACGCTCTAGGGGATGGTTT+GGG + chr4.1:28236558-28236577 MS.gene003382:intron 55.0%
!! GCCGATTTTGCGAGTTCGCT+CGG + chr4.1:28236270-28236289 MS.gene003382:CDS 55.0%
!! TTGTTCGACGCTCTAGGGGA+TGG + chr4.1:28236552-28236571 MS.gene003382:intron 55.0%
AAGGGGTAACCCGGCTTCTC+AGG + chr4.1:28236737-28236756 MS.gene003382:intron 60.0%
AGCGCGTTGTTCGACGCTCT+AGG + chr4.1:28236546-28236565 MS.gene003382:intron 60.0%
CGCGTTGTTCGACGCTCTAG+GGG + chr4.1:28236548-28236567 MS.gene003382:intron 60.0%
GCGCGTTGTTCGACGCTCTA+GGG + chr4.1:28236547-28236566 MS.gene003382:intron 60.0%
GGAACTTCACCGGAGCGAGT+TGG + chr4.1:28235889-28235908 MS.gene003382:CDS 60.0%
GTGTTCGCGTTGAGCGCGTT+TGG - chr4.1:28235602-28235621 None:intergenic 60.0%
TGGTGGAGCAGCAGCTGTTG+GGG + chr4.1:28236604-28236623 MS.gene003382:intron 60.0%
! CGTGGTGGAATACGACGCAC+AGG + chr4.1:28235687-28235706 MS.gene003382:CDS 60.0%
! GGGGATGAGTTGAAACGGGC+CGG + chr4.1:28236696-28236715 MS.gene003382:intron 60.0%
! GTTGGTGGGCCTAAGAGGAC+TGG + chr4.1:28236651-28236670 MS.gene003382:intron 60.0%
! TCGCTGTTGGTGGGCCTAAG+AGG + chr4.1:28236646-28236665 MS.gene003382:intron 60.0%
!! CGACGCTCTAGGGGATGGTT+TGG + chr4.1:28236557-28236576 MS.gene003382:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 28235592 28236885 28235592 ID=MS.gene003382
chr4.1 mRNA 28235592 28236885 28235592 ID=MS.gene003382.t1;Parent=MS.gene003382
chr4.1 exon 28235592 28236520 28235592 ID=MS.gene003382.t1.exon1;Parent=MS.gene003382.t1
chr4.1 CDS 28235592 28236520 28235592 ID=cds.MS.gene003382.t1;Parent=MS.gene003382.t1
chr4.1 exon 28236873 28236885 28236873 ID=MS.gene003382.t1.exon2;Parent=MS.gene003382.t1
chr4.1 CDS 28236873 28236885 28236873 ID=cds.MS.gene003382.t1;Parent=MS.gene003382.t1
Gene Sequence

>MS.gene003382

ATGAACACCAAACGCGCTCAACGCGAACACGATGACGACGACACCAACGTTCATGAACCATCTTTCAAAAGACCTAACTACTCTAGTGAGAAAACCGTGGTGGAATACGACGCACAGGACGACGAACAACAACAACAAGTCTTTGAACATGAAACGGAAACCGAAACCGAAGGAGAAGAATCTACAGGTCTCAAACTTCTAGGGCTTTTACTTCAATGTGCTGAGTGTGTTGCCATGGATAACCTCGACTTCGCCAACGACCTTTTACCGGAGATAACTGAACTCTCTTCACCGTTTGGAACTTCACCGGAGCGAGTTGGTGCGTATTTTGCACAAGCGTTACAAGCGCGTGTGGTGAGTTCTTGTCTCGGAAGCTACTCGCCATTAACTGCTAAATCGGTAACGCTAAATCAGTCTCAGAGAATCTTCAACGCGTTTCAATCCTACAACTCGGTTTCACCGTTGGTGAAATTCTCACACTTCACTGCGAATCAAGCAATTTTTCAGGCTTTGGATGGTGAAGATCGTGTCCATATCATTGATCTTGATATCATGCAAGGACTTCAATGGCCAGGATTGTTCCACATCCTTGCTTCGAGGTCGAAGAAGATCCGCTCGGTGAGAATTACCGGTTTCGGATCTTCTTCCGAGCTGTTAGAATCAACCGGAAGAAGACTCGCCGATTTTGCGAGTTCGCTCGGGTTACCGTTCGAGTTTCATCCGATTGAGGGTAAAATTGGAAGTGTAACTGAACCGGGTCAACTCGGTGTTAGACCGAATGAGGCAATCGTGGTTCATTGGATGCATCATTGTTTGTATGATATAACAGGAAGTGATTTAGGAACGTTGAGATTGCTGACTCAGCTGAGGCCAAAATTGATCACGACCGTTGAACAGGATCTTAGCCATGCTGGTAGCTTCTTAGCGAGGTTTGTGGAGGCTTTGCATTATTACAGCGCGTTGTTCGACGCTCTAGGGGATGGTTTGGGTGTGGATAGTGTTGAGAGGCATATGGTGGAGCAGCAGCTGTTGGGGTGTGAGATAAGGAACATTATCGCTGTTGGTGGGCCTAAGAGGACTGGTGAAGTGAAAGTTGAGAGGTGGGGGGATGAGTTGAAACGGGCCGGATTTCGACCCGTTTCATTAAGGGGTAACCCGGCTTCTCAGGCGAGTTTGTTGTTGGGGATGTTTCCTTGGAGAGGATATACATTGGTGGAAGAGAATGGTTCTCTCAAGCTTGGTTGGAAGGATCTCTCTTTGTTGATTGCTTCTGCTTGGCAGCCGTCTGATTTAA
Protein sequence

>MS.gene003382.t1

MNTKRAQREHDDDDTNVHEPSFKRPNYSSEKTVVEYDAQDDEQQQQVFEHETETETEGEESTGLKLLGLLLQCAECVAMDNLDFANDLLPEITELSSPFGTSPERVGAYFAQALQARVVSSCLGSYSPLTAKSVTLNQSQRIFNAFQSYNSVSPLVKFSHFTANQAIFQALDGEDRVHIIDLDIMQGLQWPGLFHILASRSKKIRSVRITGFGSSSELLESTGRRLADFASSLGLPFEFHPIEGKIGSVTEPGQLGVRPNEAIVVHWMHHCLYDITGSDLGTLRLLTQLRPKLITTVEQDLSHAGSFLASRLI