Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003558.t1 | RHN79076.1 | 68 | 266 | 67 | 4 | 1 | 252 | 1 | 262 | 4.00E-64 | 255 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003558.t1 | A0A396JLE5 | 68.0 | 266 | 67 | 4 | 1 | 252 | 1 | 262 | 2.9e-64 | 255.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene003558.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003558.t1 | MTR_4g073310 | 69.176 | 279 | 31 | 3 | 1 | 276 | 1 | 227 | 1.74e-114 | 328 |
| MS.gene003558.t1 | MTR_1g052185 | 42.025 | 326 | 84 | 11 | 1 | 272 | 1 | 275 | 1.18e-55 | 181 |
| MS.gene003558.t1 | MTR_1g046810 | 34.967 | 306 | 131 | 12 | 20 | 276 | 28 | 314 | 1.00e-33 | 125 |
| MS.gene003558.t1 | MTR_1g053530 | 34.826 | 201 | 104 | 7 | 23 | 222 | 25 | 199 | 3.45e-21 | 90.1 |
| MS.gene003558.t1 | MTR_6g011180 | 77.778 | 36 | 8 | 0 | 167 | 202 | 200 | 235 | 5.60e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003558.t1 | AT2G45120 | 28.682 | 129 | 80 | 1 | 19 | 135 | 180 | 308 | 8.30e-11 | 62.0 |
Find 51 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGATCATGAACATCTTAA+TGG | 0.235891 | 4.1:+30405109 | MS.gene003558:CDS |
| TTTCTCATTATCGTCCTTAA+TGG | 0.255039 | 4.1:-30404855 | None:intergenic |
| AGAAGAACATGGATTATAAA+AGG | 0.308077 | 4.1:-30404705 | None:intergenic |
| AAATTCATAACAACTCCTTT+TGG | 0.331715 | 4.1:-30405442 | None:intergenic |
| CCGCCGCTCTCATACCATTA+AGG | 0.353355 | 4.1:+30404841 | MS.gene003558:CDS |
| CCTCCTGATAGAACGTTTGT+TGG | 0.361348 | 4.1:-30405077 | None:intergenic |
| TGCGCTGCGGCTGGTGGTGG+TGG | 0.387987 | 4.1:+30405157 | MS.gene003558:CDS |
| AAACCAACAAACGTTCTATC+AGG | 0.407703 | 4.1:+30405074 | MS.gene003558:CDS |
| GCTGGTGGTGGTGGTGATCA+TGG | 0.417182 | 4.1:+30405166 | MS.gene003558:CDS |
| GGACTTTGGTGGCAAATACT+TGG | 0.426874 | 4.1:-30405047 | None:intergenic |
| AACTCACACTCATACATTCT+CGG | 0.428608 | 4.1:-30404764 | None:intergenic |
| ATGTCCGCGAAAAGCCTTGT+TGG | 0.435044 | 4.1:-30404918 | None:intergenic |
| AATAGTGAAGGCAATGGGAC+TGG | 0.441225 | 4.1:+30405391 | MS.gene003558:CDS |
| TCTTGCGCTGCGGCTGGTGG+TGG | 0.459861 | 4.1:+30405154 | MS.gene003558:CDS |
| GAGTTTGCGGCTGATATTCA+AGG | 0.475940 | 4.1:+30405318 | MS.gene003558:CDS |
| TATGATATTGCACCGTGAAA+AGG | 0.476814 | 4.1:+30404940 | MS.gene003558:CDS |
| TCTAATGAATTGAAGCCAAA+AGG | 0.489056 | 4.1:+30405427 | MS.gene003558:CDS |
| TCATGTGCAACATCTTCTGT+TGG | 0.490922 | 4.1:-30405481 | None:intergenic |
| TTGAAATTGTTCCAAGGATG+TGG | 0.492696 | 4.1:-30404987 | None:intergenic |
| AATATGTCTTGCGCTGCGGC+TGG | 0.494836 | 4.1:+30405148 | MS.gene003558:CDS |
| TCAATCGCCAACCACATCCT+TGG | 0.495376 | 4.1:+30404976 | MS.gene003558:CDS |
| GGTTGGCGATTGAATACTCT+TGG | 0.497146 | 4.1:-30404966 | None:intergenic |
| GAACAATTTCAATTTCAAGT+TGG | 0.498276 | 4.1:+30404998 | MS.gene003558:CDS |
| AAATACTTGGTTAAGTCAAT+CGG | 0.503127 | 4.1:-30405034 | None:intergenic |
| TTCAACTCTGGCAACGCACT+CGG | 0.512404 | 4.1:+30404800 | MS.gene003558:CDS |
| TTGAAATTGAAATTGTTCCA+AGG | 0.519609 | 4.1:-30404993 | None:intergenic |
| CTTGCACTCATGAGGAAGAA+AGG | 0.532255 | 4.1:+30404668 | None:intergenic |
| ATTATTATTGACCTTCACCT+TGG | 0.544902 | 4.1:-30405278 | None:intergenic |
| ATGTCTTGCGCTGCGGCTGG+TGG | 0.545212 | 4.1:+30405151 | MS.gene003558:CDS |
| GACAAGATCGAGAAACAAGT+TGG | 0.568686 | 4.1:+30405232 | MS.gene003558:CDS |
| ATAGTGAAGGCAATGGGACT+GGG | 0.570844 | 4.1:+30405392 | MS.gene003558:CDS |
| TTGGTTAAGTCAATCGGTGG+TGG | 0.571109 | 4.1:-30405028 | None:intergenic |
| TGGTGACATTGATGAAACTT+CGG | 0.577161 | 4.1:+30405252 | MS.gene003558:CDS |
| TGATGAAACTTCGGCGACCA+AGG | 0.579505 | 4.1:+30405261 | MS.gene003558:CDS |
| AGTGTGAGTTGTGCGGCAAA+AGG | 0.579769 | 4.1:+30404777 | MS.gene003558:CDS |
| TGCGGCAAAAGGTTCAACTC+TGG | 0.586813 | 4.1:+30404788 | MS.gene003558:CDS |
| TAGTTGTCCTGTTTGCAACA+AGG | 0.599547 | 4.1:+30404886 | MS.gene003558:CDS |
| TGAAGGCAATGGGACTGGGA+TGG | 0.605766 | 4.1:+30405396 | MS.gene003558:CDS |
| AACTTCGGCGACCAAGGTGA+AGG | 0.611419 | 4.1:+30405267 | MS.gene003558:CDS |
| AACTCTGGCAACGCACTCGG+TGG | 0.616672 | 4.1:+30404803 | MS.gene003558:CDS |
| CTTCAATATGTCTTGCGCTG+CGG | 0.618324 | 4.1:+30405144 | MS.gene003558:CDS |
| AATTGTTCCAAGGATGTGGT+TGG | 0.627602 | 4.1:-30404983 | None:intergenic |
| GGATTATAAAAGGAACAAGA+AGG | 0.636811 | 4.1:-30404695 | None:intergenic |
| AATGAATGAAAAGAAGAACA+TGG | 0.641497 | 4.1:-30404716 | None:intergenic |
| TACTTGGTTAAGTCAATCGG+TGG | 0.654609 | 4.1:-30405031 | None:intergenic |
| CCAACAAACGTTCTATCAGG+AGG | 0.665230 | 4.1:+30405077 | MS.gene003558:CDS |
| GCTCAAAGTTTCAAGCAATG+TGG | 0.669423 | 4.1:+30405204 | MS.gene003558:CDS |
| ATTCAAGGTTAATGACAACA+AGG | 0.683610 | 4.1:+30405333 | MS.gene003558:CDS |
| CCTTAATGGTATGAGAGCGG+CGG | 0.689827 | 4.1:-30404841 | None:intergenic |
| CGTCCTTAATGGTATGAGAG+CGG | 0.696710 | 4.1:-30404844 | None:intergenic |
| ATGTATGAGTGTGAGTTGTG+CGG | 0.703631 | 4.1:+30404770 | MS.gene003558:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATACTTGGTTAAGTCAAT+CGG | - | chr4.1:30405037-30405056 | None:intergenic | 25.0% |
| ! | AATGAATGAAAAGAAGAACA+TGG | - | chr4.1:30404719-30404738 | None:intergenic | 25.0% |
| ! | ATAACAGTAAAAAGAGTTTG+CGG | + | chr4.1:30405305-30405324 | MS.gene003558:CDS | 25.0% |
| ! | ATTGATCATGAACATCTTAA+TGG | + | chr4.1:30405109-30405128 | MS.gene003558:CDS | 25.0% |
| ! | GAAAACAAGAAAAATAGTGA+AGG | + | chr4.1:30405379-30405398 | MS.gene003558:CDS | 25.0% |
| ! | TTGAAATTGAAATTGTTCCA+AGG | - | chr4.1:30404996-30405015 | None:intergenic | 25.0% |
| !! | AAATTCATAACAACTCCTTT+TGG | - | chr4.1:30405445-30405464 | None:intergenic | 25.0% |
| !! | AGAAGAACATGGATTATAAA+AGG | - | chr4.1:30404708-30404727 | None:intergenic | 25.0% |
| !! | GAACAATTTCAATTTCAAGT+TGG | + | chr4.1:30404998-30405017 | MS.gene003558:CDS | 25.0% |
| !!! | TTTTCTTGTTTTCTTCATCT+TGG | - | chr4.1:30405372-30405391 | None:intergenic | 25.0% |
| AAGAAAAATAGTGAAGGCAA+TGG | + | chr4.1:30405385-30405404 | MS.gene003558:CDS | 30.0% | |
| AGAAAAATAGTGAAGGCAAT+GGG | + | chr4.1:30405386-30405405 | MS.gene003558:CDS | 30.0% | |
| ATTATTATTGACCTTCACCT+TGG | - | chr4.1:30405281-30405300 | None:intergenic | 30.0% | |
| ATTCAAGGTTAATGACAACA+AGG | + | chr4.1:30405333-30405352 | MS.gene003558:CDS | 30.0% | |
| GGATTATAAAAGGAACAAGA+AGG | - | chr4.1:30404698-30404717 | None:intergenic | 30.0% | |
| TCTAATGAATTGAAGCCAAA+AGG | + | chr4.1:30405427-30405446 | MS.gene003558:CDS | 30.0% | |
| TTTCTCATTATCGTCCTTAA+TGG | - | chr4.1:30404858-30404877 | None:intergenic | 30.0% | |
| !!! | AGAACGTTTGTTGGTTTTAT+GGG | - | chr4.1:30405071-30405090 | None:intergenic | 30.0% |
| !!! | TAGAACGTTTGTTGGTTTTA+TGG | - | chr4.1:30405072-30405091 | None:intergenic | 30.0% |
| AAACCAACAAACGTTCTATC+AGG | + | chr4.1:30405074-30405093 | MS.gene003558:CDS | 35.0% | |
| AACTCACACTCATACATTCT+CGG | - | chr4.1:30404767-30404786 | None:intergenic | 35.0% | |
| GAAAAAACCTTGTTGCAAAC+AGG | - | chr4.1:30404896-30404915 | None:intergenic | 35.0% | |
| TATGATATTGCACCGTGAAA+AGG | + | chr4.1:30404940-30404959 | MS.gene003558:CDS | 35.0% | |
| TGGTGACATTGATGAAACTT+CGG | + | chr4.1:30405252-30405271 | MS.gene003558:CDS | 35.0% | |
| TTGAAATTGTTCCAAGGATG+TGG | - | chr4.1:30404990-30405009 | None:intergenic | 35.0% | |
| ! | TACTCTTGGAATCCTTTTCA+CGG | - | chr4.1:30404955-30404974 | None:intergenic | 35.0% |
| !!! | CAAGGTTTTTTCATCCAACA+AGG | + | chr4.1:30404904-30404923 | MS.gene003558:CDS | 35.0% |
| !!! | TTGTTGGTTTTATGGGACTT+TGG | - | chr4.1:30405064-30405083 | None:intergenic | 35.0% |
| AATTGTTCCAAGGATGTGGT+TGG | - | chr4.1:30404986-30405005 | None:intergenic | 40.0% | |
| ATGTATGAGTGTGAGTTGTG+CGG | + | chr4.1:30404770-30404789 | MS.gene003558:CDS | 40.0% | |
| GACAAGATCGAGAAACAAGT+TGG | + | chr4.1:30405232-30405251 | MS.gene003558:CDS | 40.0% | |
| GCTCAAAGTTTCAAGCAATG+TGG | + | chr4.1:30405204-30405223 | MS.gene003558:CDS | 40.0% | |
| TACTTGGTTAAGTCAATCGG+TGG | - | chr4.1:30405034-30405053 | None:intergenic | 40.0% | |
| TAGTTGTCCTGTTTGCAACA+AGG | + | chr4.1:30404886-30404905 | MS.gene003558:CDS | 40.0% | |
| TCATGTGCAACATCTTCTGT+TGG | - | chr4.1:30405484-30405503 | None:intergenic | 40.0% | |
| !!! | TTGGTTTTATGGGACTTTGG+TGG | - | chr4.1:30405061-30405080 | None:intergenic | 40.0% |
| AATAGTGAAGGCAATGGGAC+TGG | + | chr4.1:30405391-30405410 | MS.gene003558:CDS | 45.0% | |
| ATAGTGAAGGCAATGGGACT+GGG | + | chr4.1:30405392-30405411 | MS.gene003558:CDS | 45.0% | |
| CCAACAAACGTTCTATCAGG+AGG | + | chr4.1:30405077-30405096 | MS.gene003558:CDS | 45.0% | |
| CCTCCTGATAGAACGTTTGT+TGG | - | chr4.1:30405080-30405099 | None:intergenic | 45.0% | |
| CGTCCTTAATGGTATGAGAG+CGG | - | chr4.1:30404847-30404866 | None:intergenic | 45.0% | |
| CTTCAATATGTCTTGCGCTG+CGG | + | chr4.1:30405144-30405163 | MS.gene003558:CDS | 45.0% | |
| GAGTTTGCGGCTGATATTCA+AGG | + | chr4.1:30405318-30405337 | MS.gene003558:CDS | 45.0% | |
| GGTTGGCGATTGAATACTCT+TGG | - | chr4.1:30404969-30404988 | None:intergenic | 45.0% | |
| TTGGTTAAGTCAATCGGTGG+TGG | - | chr4.1:30405031-30405050 | None:intergenic | 45.0% | |
| ! | GGACTTTGGTGGCAAATACT+TGG | - | chr4.1:30405050-30405069 | None:intergenic | 45.0% |
| ! | TCATCCAACAAGGCTTTTCG+CGG | + | chr4.1:30404914-30404933 | MS.gene003558:CDS | 45.0% |
| AGTGTGAGTTGTGCGGCAAA+AGG | + | chr4.1:30404777-30404796 | MS.gene003558:CDS | 50.0% | |
| CCTTAATGGTATGAGAGCGG+CGG | - | chr4.1:30404844-30404863 | None:intergenic | 50.0% | |
| TCAATCGCCAACCACATCCT+TGG | + | chr4.1:30404976-30404995 | MS.gene003558:CDS | 50.0% | |
| TGATGAAACTTCGGCGACCA+AGG | + | chr4.1:30405261-30405280 | MS.gene003558:CDS | 50.0% | |
| TGCGGCAAAAGGTTCAACTC+TGG | + | chr4.1:30404788-30404807 | MS.gene003558:CDS | 50.0% | |
| TTCAACTCTGGCAACGCACT+CGG | + | chr4.1:30404800-30404819 | MS.gene003558:CDS | 50.0% | |
| ! | ATGTCCGCGAAAAGCCTTGT+TGG | - | chr4.1:30404921-30404940 | None:intergenic | 50.0% |
| AACTTCGGCGACCAAGGTGA+AGG | + | chr4.1:30405267-30405286 | MS.gene003558:CDS | 55.0% | |
| AATATGTCTTGCGCTGCGGC+TGG | + | chr4.1:30405148-30405167 | MS.gene003558:CDS | 55.0% | |
| CCGCCGCTCTCATACCATTA+AGG | + | chr4.1:30404841-30404860 | MS.gene003558:CDS | 55.0% | |
| TGAAGGCAATGGGACTGGGA+TGG | + | chr4.1:30405396-30405415 | MS.gene003558:CDS | 55.0% | |
| ! | AGCGGCGGTGAGATGTTTTG+TGG | - | chr4.1:30404829-30404848 | None:intergenic | 55.0% |
| AACTCTGGCAACGCACTCGG+TGG | + | chr4.1:30404803-30404822 | MS.gene003558:CDS | 60.0% | |
| !! | GCTGGTGGTGGTGGTGATCA+TGG | + | chr4.1:30405166-30405185 | MS.gene003558:CDS | 60.0% |
| ATGTCTTGCGCTGCGGCTGG+TGG | + | chr4.1:30405151-30405170 | MS.gene003558:CDS | 65.0% | |
| TCTTGCGCTGCGGCTGGTGG+TGG | + | chr4.1:30405154-30405173 | MS.gene003558:CDS | 70.0% | |
| !! | TGCGCTGCGGCTGGTGGTGG+TGG | + | chr4.1:30405157-30405176 | MS.gene003558:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 30404677 | 30405507 | 30404677 | ID=MS.gene003558 |
| chr4.1 | mRNA | 30404677 | 30405507 | 30404677 | ID=MS.gene003558.t1;Parent=MS.gene003558 |
| chr4.1 | exon | 30404677 | 30405507 | 30404677 | ID=MS.gene003558.t1.exon1;Parent=MS.gene003558.t1 |
| chr4.1 | CDS | 30404677 | 30405507 | 30404677 | ID=cds.MS.gene003558.t1;Parent=MS.gene003558.t1 |
| Gene Sequence |
| Protein sequence |
