Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003559.t1 | RHN79076.1 | 68 | 266 | 67 | 4 | 1 | 252 | 1 | 262 | 4.00E-64 | 255 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003559.t1 | A0A396JLE5 | 68.0 | 266 | 67 | 4 | 1 | 252 | 1 | 262 | 2.9e-64 | 255.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene003559.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003559.t1 | MTR_4g073310 | 69.176 | 279 | 31 | 3 | 1 | 276 | 1 | 227 | 1.74e-114 | 328 |
| MS.gene003559.t1 | MTR_1g052185 | 42.025 | 326 | 84 | 11 | 1 | 272 | 1 | 275 | 1.18e-55 | 181 |
| MS.gene003559.t1 | MTR_1g046810 | 34.967 | 306 | 131 | 12 | 20 | 276 | 28 | 314 | 1.00e-33 | 125 |
| MS.gene003559.t1 | MTR_1g053530 | 34.826 | 201 | 104 | 7 | 23 | 222 | 25 | 199 | 3.45e-21 | 90.1 |
| MS.gene003559.t1 | MTR_6g011180 | 77.778 | 36 | 8 | 0 | 167 | 202 | 200 | 235 | 5.60e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003559.t1 | AT2G45120 | 28.682 | 129 | 80 | 1 | 19 | 135 | 180 | 308 | 8.30e-11 | 62.0 |
Find 51 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGATCATGAACATCTTAA+TGG | 0.235891 | 4.1:+30450636 | MS.gene003559:CDS |
| TTTCTCATTATCGTCCTTAA+TGG | 0.255039 | 4.1:-30450382 | None:intergenic |
| AGAAGAACATGGATTATAAA+AGG | 0.308077 | 4.1:-30450232 | None:intergenic |
| AAATTCATAACAACTCCTTT+TGG | 0.331715 | 4.1:-30450969 | None:intergenic |
| CCGCCGCTCTCATACCATTA+AGG | 0.353355 | 4.1:+30450368 | MS.gene003559:CDS |
| CCTCCTGATAGAACGTTTGT+TGG | 0.361348 | 4.1:-30450604 | None:intergenic |
| TGCGCTGCGGCTGGTGGTGG+TGG | 0.387987 | 4.1:+30450684 | MS.gene003559:CDS |
| AAACCAACAAACGTTCTATC+AGG | 0.407703 | 4.1:+30450601 | MS.gene003559:CDS |
| GCTGGTGGTGGTGGTGATCA+TGG | 0.417182 | 4.1:+30450693 | MS.gene003559:CDS |
| GGACTTTGGTGGCAAATACT+TGG | 0.426874 | 4.1:-30450574 | None:intergenic |
| AACTCACACTCATACATTCT+CGG | 0.428608 | 4.1:-30450291 | None:intergenic |
| ATGTCCGCGAAAAGCCTTGT+TGG | 0.435044 | 4.1:-30450445 | None:intergenic |
| AATAGTGAAGGCAATGGGAC+TGG | 0.441225 | 4.1:+30450918 | MS.gene003559:CDS |
| TCTTGCGCTGCGGCTGGTGG+TGG | 0.459861 | 4.1:+30450681 | MS.gene003559:CDS |
| GAGTTTGCGGCTGATATTCA+AGG | 0.475940 | 4.1:+30450845 | MS.gene003559:CDS |
| TATGATATTGCACCGTGAAA+AGG | 0.476814 | 4.1:+30450467 | MS.gene003559:CDS |
| TCTAATGAATTGAAGCCAAA+AGG | 0.489056 | 4.1:+30450954 | MS.gene003559:CDS |
| TCATGTGCAACATCTTCTGT+TGG | 0.490922 | 4.1:-30451008 | None:intergenic |
| TTGAAATTGTTCCAAGGATG+TGG | 0.492696 | 4.1:-30450514 | None:intergenic |
| AATATGTCTTGCGCTGCGGC+TGG | 0.494836 | 4.1:+30450675 | MS.gene003559:CDS |
| TCAATCGCCAACCACATCCT+TGG | 0.495376 | 4.1:+30450503 | MS.gene003559:CDS |
| GGTTGGCGATTGAATACTCT+TGG | 0.497146 | 4.1:-30450493 | None:intergenic |
| GAACAATTTCAATTTCAAGT+TGG | 0.498276 | 4.1:+30450525 | MS.gene003559:CDS |
| AAATACTTGGTTAAGTCAAT+CGG | 0.503127 | 4.1:-30450561 | None:intergenic |
| TTCAACTCTGGCAACGCACT+CGG | 0.512404 | 4.1:+30450327 | MS.gene003559:CDS |
| TTGAAATTGAAATTGTTCCA+AGG | 0.519609 | 4.1:-30450520 | None:intergenic |
| CTTGCACTCATGAGGAAGAA+AGG | 0.532255 | 4.1:+30450195 | None:intergenic |
| ATTATTATTGACCTTCACCT+TGG | 0.544902 | 4.1:-30450805 | None:intergenic |
| ATGTCTTGCGCTGCGGCTGG+TGG | 0.545212 | 4.1:+30450678 | MS.gene003559:CDS |
| GACAAGATCGAGAAACAAGT+TGG | 0.568686 | 4.1:+30450759 | MS.gene003559:CDS |
| ATAGTGAAGGCAATGGGACT+GGG | 0.570844 | 4.1:+30450919 | MS.gene003559:CDS |
| TTGGTTAAGTCAATCGGTGG+TGG | 0.571109 | 4.1:-30450555 | None:intergenic |
| TGGTGACATTGATGAAACTT+CGG | 0.577161 | 4.1:+30450779 | MS.gene003559:CDS |
| TGATGAAACTTCGGCGACCA+AGG | 0.579505 | 4.1:+30450788 | MS.gene003559:CDS |
| AGTGTGAGTTGTGCGGCAAA+AGG | 0.579769 | 4.1:+30450304 | MS.gene003559:CDS |
| TGCGGCAAAAGGTTCAACTC+TGG | 0.586813 | 4.1:+30450315 | MS.gene003559:CDS |
| TAGTTGTCCTGTTTGCAACA+AGG | 0.599547 | 4.1:+30450413 | MS.gene003559:CDS |
| TGAAGGCAATGGGACTGGGA+TGG | 0.605766 | 4.1:+30450923 | MS.gene003559:CDS |
| AACTTCGGCGACCAAGGTGA+AGG | 0.611419 | 4.1:+30450794 | MS.gene003559:CDS |
| AACTCTGGCAACGCACTCGG+TGG | 0.616672 | 4.1:+30450330 | MS.gene003559:CDS |
| CTTCAATATGTCTTGCGCTG+CGG | 0.618324 | 4.1:+30450671 | MS.gene003559:CDS |
| AATTGTTCCAAGGATGTGGT+TGG | 0.627602 | 4.1:-30450510 | None:intergenic |
| GGATTATAAAAGGAACAAGA+AGG | 0.636811 | 4.1:-30450222 | None:intergenic |
| AATGAATGAAAAGAAGAACA+TGG | 0.641497 | 4.1:-30450243 | None:intergenic |
| TACTTGGTTAAGTCAATCGG+TGG | 0.654609 | 4.1:-30450558 | None:intergenic |
| CCAACAAACGTTCTATCAGG+AGG | 0.665230 | 4.1:+30450604 | MS.gene003559:CDS |
| GCTCAAAGTTTCAAGCAATG+TGG | 0.669423 | 4.1:+30450731 | MS.gene003559:CDS |
| ATTCAAGGTTAATGACAACA+AGG | 0.683610 | 4.1:+30450860 | MS.gene003559:CDS |
| CCTTAATGGTATGAGAGCGG+CGG | 0.689827 | 4.1:-30450368 | None:intergenic |
| CGTCCTTAATGGTATGAGAG+CGG | 0.696710 | 4.1:-30450371 | None:intergenic |
| ATGTATGAGTGTGAGTTGTG+CGG | 0.703631 | 4.1:+30450297 | MS.gene003559:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATACTTGGTTAAGTCAAT+CGG | - | chr4.1:30450564-30450583 | None:intergenic | 25.0% |
| ! | AATGAATGAAAAGAAGAACA+TGG | - | chr4.1:30450246-30450265 | None:intergenic | 25.0% |
| ! | ATAACAGTAAAAAGAGTTTG+CGG | + | chr4.1:30450832-30450851 | MS.gene003559:CDS | 25.0% |
| ! | ATTGATCATGAACATCTTAA+TGG | + | chr4.1:30450636-30450655 | MS.gene003559:CDS | 25.0% |
| ! | GAAAACAAGAAAAATAGTGA+AGG | + | chr4.1:30450906-30450925 | MS.gene003559:CDS | 25.0% |
| ! | TTGAAATTGAAATTGTTCCA+AGG | - | chr4.1:30450523-30450542 | None:intergenic | 25.0% |
| !! | AAATTCATAACAACTCCTTT+TGG | - | chr4.1:30450972-30450991 | None:intergenic | 25.0% |
| !! | AGAAGAACATGGATTATAAA+AGG | - | chr4.1:30450235-30450254 | None:intergenic | 25.0% |
| !! | GAACAATTTCAATTTCAAGT+TGG | + | chr4.1:30450525-30450544 | MS.gene003559:CDS | 25.0% |
| !!! | TTTTCTTGTTTTCTTCATCT+TGG | - | chr4.1:30450899-30450918 | None:intergenic | 25.0% |
| AAGAAAAATAGTGAAGGCAA+TGG | + | chr4.1:30450912-30450931 | MS.gene003559:CDS | 30.0% | |
| AGAAAAATAGTGAAGGCAAT+GGG | + | chr4.1:30450913-30450932 | MS.gene003559:CDS | 30.0% | |
| ATTATTATTGACCTTCACCT+TGG | - | chr4.1:30450808-30450827 | None:intergenic | 30.0% | |
| ATTCAAGGTTAATGACAACA+AGG | + | chr4.1:30450860-30450879 | MS.gene003559:CDS | 30.0% | |
| GGATTATAAAAGGAACAAGA+AGG | - | chr4.1:30450225-30450244 | None:intergenic | 30.0% | |
| TCTAATGAATTGAAGCCAAA+AGG | + | chr4.1:30450954-30450973 | MS.gene003559:CDS | 30.0% | |
| TTTCTCATTATCGTCCTTAA+TGG | - | chr4.1:30450385-30450404 | None:intergenic | 30.0% | |
| !!! | AGAACGTTTGTTGGTTTTAT+GGG | - | chr4.1:30450598-30450617 | None:intergenic | 30.0% |
| !!! | TAGAACGTTTGTTGGTTTTA+TGG | - | chr4.1:30450599-30450618 | None:intergenic | 30.0% |
| AAACCAACAAACGTTCTATC+AGG | + | chr4.1:30450601-30450620 | MS.gene003559:CDS | 35.0% | |
| AACTCACACTCATACATTCT+CGG | - | chr4.1:30450294-30450313 | None:intergenic | 35.0% | |
| GAAAAAACCTTGTTGCAAAC+AGG | - | chr4.1:30450423-30450442 | None:intergenic | 35.0% | |
| TATGATATTGCACCGTGAAA+AGG | + | chr4.1:30450467-30450486 | MS.gene003559:CDS | 35.0% | |
| TGGTGACATTGATGAAACTT+CGG | + | chr4.1:30450779-30450798 | MS.gene003559:CDS | 35.0% | |
| TTGAAATTGTTCCAAGGATG+TGG | - | chr4.1:30450517-30450536 | None:intergenic | 35.0% | |
| ! | TACTCTTGGAATCCTTTTCA+CGG | - | chr4.1:30450482-30450501 | None:intergenic | 35.0% |
| !!! | CAAGGTTTTTTCATCCAACA+AGG | + | chr4.1:30450431-30450450 | MS.gene003559:CDS | 35.0% |
| !!! | TTGTTGGTTTTATGGGACTT+TGG | - | chr4.1:30450591-30450610 | None:intergenic | 35.0% |
| AATTGTTCCAAGGATGTGGT+TGG | - | chr4.1:30450513-30450532 | None:intergenic | 40.0% | |
| ATGTATGAGTGTGAGTTGTG+CGG | + | chr4.1:30450297-30450316 | MS.gene003559:CDS | 40.0% | |
| GACAAGATCGAGAAACAAGT+TGG | + | chr4.1:30450759-30450778 | MS.gene003559:CDS | 40.0% | |
| GCTCAAAGTTTCAAGCAATG+TGG | + | chr4.1:30450731-30450750 | MS.gene003559:CDS | 40.0% | |
| TACTTGGTTAAGTCAATCGG+TGG | - | chr4.1:30450561-30450580 | None:intergenic | 40.0% | |
| TAGTTGTCCTGTTTGCAACA+AGG | + | chr4.1:30450413-30450432 | MS.gene003559:CDS | 40.0% | |
| TCATGTGCAACATCTTCTGT+TGG | - | chr4.1:30451011-30451030 | None:intergenic | 40.0% | |
| !!! | TTGGTTTTATGGGACTTTGG+TGG | - | chr4.1:30450588-30450607 | None:intergenic | 40.0% |
| AATAGTGAAGGCAATGGGAC+TGG | + | chr4.1:30450918-30450937 | MS.gene003559:CDS | 45.0% | |
| ATAGTGAAGGCAATGGGACT+GGG | + | chr4.1:30450919-30450938 | MS.gene003559:CDS | 45.0% | |
| CCAACAAACGTTCTATCAGG+AGG | + | chr4.1:30450604-30450623 | MS.gene003559:CDS | 45.0% | |
| CCTCCTGATAGAACGTTTGT+TGG | - | chr4.1:30450607-30450626 | None:intergenic | 45.0% | |
| CGTCCTTAATGGTATGAGAG+CGG | - | chr4.1:30450374-30450393 | None:intergenic | 45.0% | |
| CTTCAATATGTCTTGCGCTG+CGG | + | chr4.1:30450671-30450690 | MS.gene003559:CDS | 45.0% | |
| GAGTTTGCGGCTGATATTCA+AGG | + | chr4.1:30450845-30450864 | MS.gene003559:CDS | 45.0% | |
| GGTTGGCGATTGAATACTCT+TGG | - | chr4.1:30450496-30450515 | None:intergenic | 45.0% | |
| TTGGTTAAGTCAATCGGTGG+TGG | - | chr4.1:30450558-30450577 | None:intergenic | 45.0% | |
| ! | GGACTTTGGTGGCAAATACT+TGG | - | chr4.1:30450577-30450596 | None:intergenic | 45.0% |
| ! | TCATCCAACAAGGCTTTTCG+CGG | + | chr4.1:30450441-30450460 | MS.gene003559:CDS | 45.0% |
| AGTGTGAGTTGTGCGGCAAA+AGG | + | chr4.1:30450304-30450323 | MS.gene003559:CDS | 50.0% | |
| CCTTAATGGTATGAGAGCGG+CGG | - | chr4.1:30450371-30450390 | None:intergenic | 50.0% | |
| TCAATCGCCAACCACATCCT+TGG | + | chr4.1:30450503-30450522 | MS.gene003559:CDS | 50.0% | |
| TGATGAAACTTCGGCGACCA+AGG | + | chr4.1:30450788-30450807 | MS.gene003559:CDS | 50.0% | |
| TGCGGCAAAAGGTTCAACTC+TGG | + | chr4.1:30450315-30450334 | MS.gene003559:CDS | 50.0% | |
| TTCAACTCTGGCAACGCACT+CGG | + | chr4.1:30450327-30450346 | MS.gene003559:CDS | 50.0% | |
| ! | ATGTCCGCGAAAAGCCTTGT+TGG | - | chr4.1:30450448-30450467 | None:intergenic | 50.0% |
| AACTTCGGCGACCAAGGTGA+AGG | + | chr4.1:30450794-30450813 | MS.gene003559:CDS | 55.0% | |
| AATATGTCTTGCGCTGCGGC+TGG | + | chr4.1:30450675-30450694 | MS.gene003559:CDS | 55.0% | |
| CCGCCGCTCTCATACCATTA+AGG | + | chr4.1:30450368-30450387 | MS.gene003559:CDS | 55.0% | |
| TGAAGGCAATGGGACTGGGA+TGG | + | chr4.1:30450923-30450942 | MS.gene003559:CDS | 55.0% | |
| ! | AGCGGCGGTGAGATGTTTTG+TGG | - | chr4.1:30450356-30450375 | None:intergenic | 55.0% |
| AACTCTGGCAACGCACTCGG+TGG | + | chr4.1:30450330-30450349 | MS.gene003559:CDS | 60.0% | |
| !! | GCTGGTGGTGGTGGTGATCA+TGG | + | chr4.1:30450693-30450712 | MS.gene003559:CDS | 60.0% |
| ATGTCTTGCGCTGCGGCTGG+TGG | + | chr4.1:30450678-30450697 | MS.gene003559:CDS | 65.0% | |
| TCTTGCGCTGCGGCTGGTGG+TGG | + | chr4.1:30450681-30450700 | MS.gene003559:CDS | 70.0% | |
| !! | TGCGCTGCGGCTGGTGGTGG+TGG | + | chr4.1:30450684-30450703 | MS.gene003559:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 30450204 | 30451034 | 30450204 | ID=MS.gene003559 |
| chr4.1 | mRNA | 30450204 | 30451034 | 30450204 | ID=MS.gene003559.t1;Parent=MS.gene003559 |
| chr4.1 | exon | 30450204 | 30451034 | 30450204 | ID=MS.gene003559.t1.exon1;Parent=MS.gene003559.t1 |
| chr4.1 | CDS | 30450204 | 30451034 | 30450204 | ID=cds.MS.gene003559.t1;Parent=MS.gene003559.t1 |
| Gene Sequence |
| Protein sequence |
