AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003566

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003566.t1 MTR_4g073100 99.251 267 2 0 1 267 1 267 0.0 554
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003566.t1 AT4G35040 64.576 271 80 8 1 266 1 260 6.96e-112 323
MS.gene003566.t1 AT4G35040 64.576 271 80 8 1 266 1 260 6.96e-112 323
MS.gene003566.t1 AT4G35040 64.576 271 80 8 1 266 1 260 6.96e-112 323
MS.gene003566.t1 AT4G35040 64.576 271 80 8 1 266 1 260 6.96e-112 323
MS.gene003566.t1 AT2G16770 62.547 267 80 6 1 266 1 248 8.78e-112 322
MS.gene003566.t1 AT2G16770 62.547 267 80 6 1 266 1 248 8.78e-112 322
MS.gene003566.t1 AT3G51960 55.224 134 53 3 39 171 13 140 5.61e-46 152
MS.gene003566.t1 AT3G51960 55.224 134 53 3 39 171 52 179 1.24e-45 153
MS.gene003566.t1 AT3G51960 52.985 134 55 2 39 171 52 178 3.38e-44 149

Find 81 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAGGATTGAAGGGGAAAT+TGG 0.203175 4.1:+30535716 MS.gene003566:CDS
CCCAACCGGTCGCTTCTTAC+TGG 0.266035 4.1:-30535519 None:intergenic
GATCCTTGAGCCCCAAATTC+TGG 0.270466 4.1:-30535940 None:intergenic
GTGTATGGGAGGCCAGAATT+TGG 0.299733 4.1:+30535928 MS.gene003566:CDS
TGTATGGGAGGCCAGAATTT+GGG 0.316343 4.1:+30535929 MS.gene003566:CDS
AGTGTTTGTTGGTTGATATA+AGG 0.329657 4.1:+30535693 MS.gene003566:CDS
GTTGCAAGGGCAGGCTGCTT+TGG 0.335855 4.1:+30535649 MS.gene003566:CDS
ATATATTCTTCATCCGGCTT+TGG 0.355159 4.1:-30536867 None:intergenic
GTTTGAAATTCACCCATGTT+TGG 0.360095 4.1:-30535302 None:intergenic
GTGTTTGTTGGTTGATATAA+GGG 0.361837 4.1:+30535694 MS.gene003566:CDS
GGAGGATTGAAGGGGAAATT+GGG 0.370796 4.1:+30535717 MS.gene003566:CDS
CTCAAGGATCTTCGTGGTTG+TGG 0.376487 4.1:+30535953 MS.gene003566:CDS
ATTTCAAACAAGTGGTTCAA+TGG 0.405614 4.1:+30535316 MS.gene003566:CDS
GTTTGAGAGTCTCCAGTGTA+TGG 0.417641 4.1:+30535913 MS.gene003566:CDS
TGCGAGGTTGAAGTGTTTGT+TGG 0.420725 4.1:+30535682 MS.gene003566:CDS
ACATTCGCCGGTTTCTGATA+CGG 0.425585 4.1:-30535746 None:intergenic
GCAAGGGCAGGCTGCTTTGG+AGG 0.426440 4.1:+30535652 MS.gene003566:CDS
GGCTGCGTCGTTGGAGGATG+AGG 0.428168 4.1:+30535586 MS.gene003566:CDS
TCATCACACTGCACATTACA+AGG 0.429810 4.1:-30535809 None:intergenic
CAACTTATATATTCTTCATC+CGG 0.430557 4.1:-30536873 None:intergenic
GATCTTCGTGGTTGTGGAGG+TGG 0.440100 4.1:+30535959 MS.gene003566:CDS
TCAGCAGCAATCTTCCCATC+TGG 0.456534 4.1:-30535851 None:intergenic
GTCATTTCCGTATCAGAAAC+CGG 0.461463 4.1:+30535739 MS.gene003566:CDS
GAAAGCTAGGGCTGCGTCGT+TGG 0.478279 4.1:+30535577 MS.gene003566:CDS
TCGTGGTTGTGGAGGTGGAC+CGG 0.485043 4.1:+30535964 MS.gene003566:CDS
GGGTCTCGTGCAGCCAAAGC+CGG 0.486937 4.1:+30536854 MS.gene003566:CDS
AGCTAGGGCTGCGTCGTTGG+AGG 0.489293 4.1:+30535580 MS.gene003566:CDS
CTGCTGAAGATGATCAGGTT+GGG 0.489651 4.1:+30535462 MS.gene003566:CDS
AGGCCAGAATTTGGGGCTCA+AGG 0.491612 4.1:+30535937 MS.gene003566:CDS
CAAGCTTCCAGTAAAAGAAA+AGG 0.495698 4.1:+30536007 MS.gene003566:CDS
ACGGCCTCTTTATTCCCAAC+CGG 0.498176 4.1:-30535533 None:intergenic
ATCTTCAGCAGGGACAATCT+TGG 0.499105 4.1:-30535450 None:intergenic
TTGGGGCTCAAGGATCTTCG+TGG 0.500226 4.1:+30535947 MS.gene003566:CDS
CCATCGAATCCATCCTCATT+CGG 0.507560 4.1:-30535884 None:intergenic
TTGGGAATAAAGAGGCCGTT+AGG 0.508441 4.1:+30535537 MS.gene003566:CDS
GCATATTGCCTTCGTCCAGA+TGG 0.514127 4.1:+30535836 MS.gene003566:CDS
TGTCCCTGCTGAAGATGATC+AGG 0.516014 4.1:+30535457 MS.gene003566:CDS
CCAGTAAGAAGCGACCGGTT+GGG 0.517346 4.1:+30535519 MS.gene003566:CDS
ATCGTGAGAAGAAGAAAGCT+AGG 0.518063 4.1:+30535564 MS.gene003566:CDS
TAATGTGCAGTGTGATGATC+GGG 0.521659 4.1:+30535814 MS.gene003566:CDS
CTTTGGAGGCCGAAGTTGCG+AGG 0.525689 4.1:+30535666 MS.gene003566:CDS
CCAACCTGATCATCTTCAGC+AGG 0.525807 4.1:-30535461 None:intergenic
GATGAGGAAGTTGCAAGGGC+AGG 0.525833 4.1:+30535640 MS.gene003566:CDS
TCCAGTAAGAAGCGACCGGT+TGG 0.526553 4.1:+30535518 MS.gene003566:CDS
AGGATGAGGTCATGAAATTG+AGG 0.534541 4.1:+30535600 MS.gene003566:CDS
ATGGGTGAATTTCAAACAAG+TGG 0.547703 4.1:+30535308 MS.gene003566:CDS
CATATTGCCTTCGTCCAGAT+GGG 0.548979 4.1:+30535837 MS.gene003566:CDS
AATTCTGGCCTCCCATACAC+TGG 0.551814 4.1:-30535925 None:intergenic
AATCCCGCGATGAATTTGCC+TGG 0.554148 4.1:+30535770 MS.gene003566:CDS
GTGGCGTCGCCGAATGAGGA+TGG 0.554803 4.1:+30535875 MS.gene003566:CDS
GACCAGGTGGGTTACACGTG+TGG 0.559695 4.1:-30535388 None:intergenic
ATCGCGGGATTCACATTCGC+CGG 0.559991 4.1:-30535758 None:intergenic
ACACTTCAACCTCGCAACTT+CGG 0.570082 4.1:-30535675 None:intergenic
GGGTTCATCACATGAGAACC+AGG 0.577391 4.1:-30535788 None:intergenic
GTTTGTTGGTTGATATAAGG+GGG 0.577819 4.1:+30535696 MS.gene003566:CDS
TGTTTGTTGGTTGATATAAG+GGG 0.578417 4.1:+30535695 MS.gene003566:CDS
GGATGAGGTCATGAAATTGA+GGG 0.578672 4.1:+30535601 MS.gene003566:CDS
GTAATGTGCAGTGTGATGAT+CGG 0.581966 4.1:+30535813 MS.gene003566:CDS
GAAGTCCAGTAAGAAGCGAC+CGG 0.585566 4.1:+30535514 MS.gene003566:CDS
TTTGAGAGTCTCCAGTGTAT+GGG 0.588557 4.1:+30535914 MS.gene003566:CDS
GTATGGGAGGCCAGAATTTG+GGG 0.595109 4.1:+30535930 MS.gene003566:CDS
ATTTGATGAGGAAGTTGCAA+GGG 0.596253 4.1:+30535636 MS.gene003566:CDS
CCGAATGAGGATGGATTCGA+TGG 0.597469 4.1:+30535884 MS.gene003566:CDS
TGAAGTGGCGTCGCCGAATG+AGG 0.598815 4.1:+30535871 MS.gene003566:CDS
AAGGATCTTCGTGGTTGTGG+AGG 0.602836 4.1:+30535956 MS.gene003566:CDS
CATTTGATGAGGAAGTTGCA+AGG 0.604562 4.1:+30535635 MS.gene003566:CDS
ACCCACACGTGTAACCCACC+TGG 0.607124 4.1:+30535386 MS.gene003566:CDS
ACCAGGTGGGTTACACGTGT+GGG 0.610384 4.1:-30535387 None:intergenic
GAACCAGGCAAATTCATCGC+GGG 0.610422 4.1:-30535773 None:intergenic
TCGTGAGAAGAAGAAAGCTA+GGG 0.612179 4.1:+30535565 MS.gene003566:CDS
TGGGAAGATTGCTGCTGAAG+TGG 0.614233 4.1:+30535856 MS.gene003566:CDS
CTTTGAATCAGCATTTGATG+AGG 0.616238 4.1:+30535624 MS.gene003566:CDS
CAATCTTCCCATCTGGACGA+AGG 0.622375 4.1:-30535844 None:intergenic
CCTGCTGAAGATGATCAGGT+TGG 0.627520 4.1:+30535461 MS.gene003566:CDS
GAGAGTCTCCAGTGTATGGG+AGG 0.629740 4.1:+30535917 MS.gene003566:CDS
CTTCTCACGATACTTCCTAA+CGG 0.630373 4.1:-30535552 None:intergenic
GCGACCGGTTGGGAATAAAG+AGG 0.649541 4.1:+30535529 MS.gene003566:CDS
CAACCTGATCATCTTCAGCA+GGG 0.684116 4.1:-30535460 None:intergenic
CATCACACTGCACATTACAA+GGG 0.696358 4.1:-30535808 None:intergenic
AGAACCAGGCAAATTCATCG+CGG 0.732891 4.1:-30535774 None:intergenic
GAATTGACATTAGACAACGC+CGG 0.756510 4.1:-30535983 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACAGATCTTTAATTAACA+GGG + chr4.1:30536831-30536850 MS.gene003566:intron 20.0%
!!! TTTTTTTGATGAATTGTTGA+AGG + chr4.1:30535343-30535362 MS.gene003566:CDS 20.0%
! AACAGATCTTTAATTAACAG+GGG + chr4.1:30536832-30536851 MS.gene003566:intron 25.0%
! ACATAATGCAAAAACAAACA+AGG - chr4.1:30536285-30536304 None:intergenic 25.0%
! CATAATGCAAAAACAAACAA+GGG - chr4.1:30536284-30536303 None:intergenic 25.0%
! CTAACAGATCTTTAATTAAC+AGG + chr4.1:30536830-30536849 MS.gene003566:intron 25.0%
! TATAAGTATGAGTTAACAAG+TGG + chr4.1:30536554-30536573 MS.gene003566:intron 25.0%
! TTGGAGAAATTGAAATTTGA+AGG + chr4.1:30536607-30536626 MS.gene003566:intron 25.0%
!! TCATACTTATATAAAGCATC+TGG - chr4.1:30536545-30536564 None:intergenic 25.0%
AACAAAAGCCTTTCCATATA+AGG - chr4.1:30536344-30536363 None:intergenic 30.0%
ACAGATCTTTAATTAACAGG+GGG + chr4.1:30536833-30536852 MS.gene003566:intron 30.0%
ATTTCAAACAAGTGGTTCAA+TGG + chr4.1:30535316-30535335 MS.gene003566:CDS 30.0%
TGGAAGATCTTGTCATAATT+TGG - chr4.1:30536582-30536601 None:intergenic 30.0%
TTGCTTCTCATTCTGTAAAA+TGG + chr4.1:30536806-30536825 MS.gene003566:intron 30.0%
TTTCAATTTCTCCAATCTAC+TGG - chr4.1:30536602-30536621 None:intergenic 30.0%
! AGTGTTTGTTGGTTGATATA+AGG + chr4.1:30535693-30535712 MS.gene003566:CDS 30.0%
! GTGTTTGTTGGTTGATATAA+GGG + chr4.1:30535694-30535713 MS.gene003566:CDS 30.0%
! TGTTTGTTGGTTGATATAAG+GGG + chr4.1:30535695-30535714 MS.gene003566:CDS 30.0%
!! AGAGTTCTTGTTAGTTTGTA+TGG + chr4.1:30536518-30536537 MS.gene003566:intron 30.0%
!! AGTCTCAACTTTTTAGTACA+GGG - chr4.1:30536671-30536690 None:intergenic 30.0%
!! TAGTCTCAACTTTTTAGTAC+AGG - chr4.1:30536672-30536691 None:intergenic 30.0%
!!! AGCATTTTTTGTCATGGTTT+GGG + chr4.1:30536139-30536158 MS.gene003566:intron 30.0%
!!! TAGCATTTTTTGTCATGGTT+TGG + chr4.1:30536138-30536157 MS.gene003566:intron 30.0%
ACGAGACAAACAATCATCAT+TGG - chr4.1:30536167-30536186 None:intergenic 35.0%
ATGCCCTAAAGTTATCAAAC+CGG - chr4.1:30536767-30536786 None:intergenic 35.0%
ATGGGTGAATTTCAAACAAG+TGG + chr4.1:30535308-30535327 MS.gene003566:CDS 35.0%
ATTTGATGAGGAAGTTGCAA+GGG + chr4.1:30535636-30535655 MS.gene003566:CDS 35.0%
CAAGCTTCCAGTAAAAGAAA+AGG + chr4.1:30536007-30536026 MS.gene003566:CDS 35.0%
CAATCACAATTGCTTTGCTT+TGG - chr4.1:30536741-30536760 None:intergenic 35.0%
CAGATCTTTAATTAACAGGG+GGG + chr4.1:30536834-30536853 MS.gene003566:intron 35.0%
CTTTGAATCAGCATTTGATG+AGG + chr4.1:30535624-30535643 MS.gene003566:CDS 35.0%
TGCACATACTCTACTAGAAA+GGG + chr4.1:30536043-30536062 MS.gene003566:intron 35.0%
TTAAAATCCGGAATGAGCAA+GGG - chr4.1:30536458-30536477 None:intergenic 35.0%
! AAAGCAATTGTGATTGACTC+CGG + chr4.1:30536745-30536764 MS.gene003566:intron 35.0%
! ACTCCGGTTTGATAACTTTA+GGG + chr4.1:30536761-30536780 MS.gene003566:intron 35.0%
! GTCATCACCTTTTCTTTTAC+TGG - chr4.1:30536017-30536036 None:intergenic 35.0%
! GTTTGAAATTCACCCATGTT+TGG - chr4.1:30535305-30535324 None:intergenic 35.0%
! GTTTGTTGGTTGATATAAGG+GGG + chr4.1:30535696-30535715 MS.gene003566:CDS 35.0%
! TGCTCATTCCGGATTTTAAA+GGG + chr4.1:30536459-30536478 MS.gene003566:intron 35.0%
! TTGCTCATTCCGGATTTTAA+AGG + chr4.1:30536458-30536477 MS.gene003566:intron 35.0%
! TTTAAAATCCGGAATGAGCA+AGG - chr4.1:30536459-30536478 None:intergenic 35.0%
!! ACTAGACTCCCTTTAAAATC+CGG - chr4.1:30536470-30536489 None:intergenic 35.0%
!! GTCTCAACTTTTTAGTACAG+GGG - chr4.1:30536670-30536689 None:intergenic 35.0%
!! TCTCAACTTTTTAGTACAGG+GGG - chr4.1:30536669-30536688 None:intergenic 35.0%
!!! GTGTCTAGCATTTTTTGTCA+TGG + chr4.1:30536133-30536152 MS.gene003566:intron 35.0%
AATGTTACCCTTGCTCATTC+CGG + chr4.1:30536448-30536467 MS.gene003566:intron 40.0%
AGGATGAGGTCATGAAATTG+AGG + chr4.1:30535600-30535619 MS.gene003566:CDS 40.0%
ATATAAGGGGGAGGATTGAA+GGG + chr4.1:30535708-30535727 MS.gene003566:CDS 40.0%
ATCGTGAGAAGAAGAAAGCT+AGG + chr4.1:30535564-30535583 MS.gene003566:CDS 40.0%
CATCACACTGCACATTACAA+GGG - chr4.1:30535811-30535830 None:intergenic 40.0%
CATTTGATGAGGAAGTTGCA+AGG + chr4.1:30535635-30535654 MS.gene003566:CDS 40.0%
CTTCTCACGATACTTCCTAA+CGG - chr4.1:30535555-30535574 None:intergenic 40.0%
GAATTGACATTAGACAACGC+CGG - chr4.1:30535986-30536005 None:intergenic 40.0%
GACAAGATCTTCCAGTAGAT+TGG + chr4.1:30536588-30536607 MS.gene003566:intron 40.0%
GGATGAGGTCATGAAATTGA+GGG + chr4.1:30535601-30535620 MS.gene003566:CDS 40.0%
GTAATGTGCAGTGTGATGAT+CGG + chr4.1:30535813-30535832 MS.gene003566:CDS 40.0%
GTCATTTCCGTATCAGAAAC+CGG + chr4.1:30535739-30535758 MS.gene003566:CDS 40.0%
GTGCACATACTCTACTAGAA+AGG + chr4.1:30536042-30536061 MS.gene003566:intron 40.0%
GTGTGAGTGTGTGAAAAATC+AGG - chr4.1:30535413-30535432 None:intergenic 40.0%
TAATGTGCAGTGTGATGATC+GGG + chr4.1:30535814-30535833 MS.gene003566:CDS 40.0%
TCATCACACTGCACATTACA+AGG - chr4.1:30535812-30535831 None:intergenic 40.0%
TCGTGAGAAGAAGAAAGCTA+GGG + chr4.1:30535565-30535584 MS.gene003566:CDS 40.0%
TGACTCTGCCTTATATGGAA+AGG + chr4.1:30536333-30536352 MS.gene003566:intron 40.0%
TTTGAGAGTCTCCAGTGTAT+GGG + chr4.1:30535914-30535933 MS.gene003566:CDS 40.0%
! GACTCCGGTTTGATAACTTT+AGG + chr4.1:30536760-30536779 MS.gene003566:intron 40.0%
!! CTCAACTTTTTAGTACAGGG+GGG - chr4.1:30536668-30536687 None:intergenic 40.0%
!!! GTGTTTTCGAGTCCAAACAT+GGG + chr4.1:30535290-30535309 MS.gene003566:CDS 40.0%
ACACTTCAACCTCGCAACTT+CGG - chr4.1:30535678-30535697 None:intergenic 45.0%
AGAACCAGGCAAATTCATCG+CGG - chr4.1:30535777-30535796 None:intergenic 45.0%
ATCTTCAGCAGGGACAATCT+TGG - chr4.1:30535453-30535472 None:intergenic 45.0%
CAACCTGATCATCTTCAGCA+GGG - chr4.1:30535463-30535482 None:intergenic 45.0%
CATATTGCCTTCGTCCAGAT+GGG + chr4.1:30535837-30535856 MS.gene003566:CDS 45.0%
CCATCGAATCCATCCTCATT+CGG - chr4.1:30535887-30535906 None:intergenic 45.0%
CTGCTGAAGATGATCAGGTT+GGG + chr4.1:30535462-30535481 MS.gene003566:CDS 45.0%
GATATAAGGGGGAGGATTGA+AGG + chr4.1:30535707-30535726 MS.gene003566:CDS 45.0%
GGAGGATTGAAGGGGAAATT+GGG + chr4.1:30535717-30535736 MS.gene003566:CDS 45.0%
GTGAAAAATCAGGACCAGGT+GGG - chr4.1:30535403-30535422 None:intergenic 45.0%
GTGTGTGAAAAATCAGGACC+AGG - chr4.1:30535407-30535426 None:intergenic 45.0%
GTTTGAGAGTCTCCAGTGTA+TGG + chr4.1:30535913-30535932 MS.gene003566:CDS 45.0%
TATAAGGGGGAGGATTGAAG+GGG + chr4.1:30535709-30535728 MS.gene003566:CDS 45.0%
TGTATGGGAGGCCAGAATTT+GGG + chr4.1:30535929-30535948 MS.gene003566:CDS 45.0%
TGTGAAAAATCAGGACCAGG+TGG - chr4.1:30535404-30535423 None:intergenic 45.0%
TTGGGAATAAAGAGGCCGTT+AGG + chr4.1:30535537-30535556 MS.gene003566:CDS 45.0%
! GTAGCTGACTCTGCCTTATA+TGG + chr4.1:30536328-30536347 MS.gene003566:intron 45.0%
! TGCGAGGTTGAAGTGTTTGT+TGG + chr4.1:30535682-30535701 MS.gene003566:CDS 45.0%
! TGTTGGTTGATATAAGGGGG+AGG + chr4.1:30535699-30535718 MS.gene003566:CDS 45.0%
!! ACATTCGCCGGTTTCTGATA+CGG - chr4.1:30535749-30535768 None:intergenic 45.0%
!!! CGTGTTTTCGAGTCCAAACA+TGG + chr4.1:30535289-30535308 MS.gene003566:CDS 45.0%
AAGGATCTTCGTGGTTGTGG+AGG + chr4.1:30535956-30535975 MS.gene003566:CDS 50.0%
AATCCCGCGATGAATTTGCC+TGG + chr4.1:30535770-30535789 MS.gene003566:CDS 50.0%
AATTCTGGCCTCCCATACAC+TGG - chr4.1:30535928-30535947 None:intergenic 50.0%
ACGGCCTCTTTATTCCCAAC+CGG - chr4.1:30535536-30535555 None:intergenic 50.0%
CAATCTTCCCATCTGGACGA+AGG - chr4.1:30535847-30535866 None:intergenic 50.0%
CCAACCTGATCATCTTCAGC+AGG - chr4.1:30535464-30535483 None:intergenic 50.0%
CCTGCTGAAGATGATCAGGT+TGG + chr4.1:30535461-30535480 MS.gene003566:CDS 50.0%
CTCAAGGATCTTCGTGGTTG+TGG + chr4.1:30535953-30535972 MS.gene003566:CDS 50.0%
GAACCAGGCAAATTCATCGC+GGG - chr4.1:30535776-30535795 None:intergenic 50.0%
GAAGTCCAGTAAGAAGCGAC+CGG + chr4.1:30535514-30535533 MS.gene003566:CDS 50.0%
GATCCTTGAGCCCCAAATTC+TGG - chr4.1:30535943-30535962 None:intergenic 50.0%
GCATATTGCCTTCGTCCAGA+TGG + chr4.1:30535836-30535855 MS.gene003566:CDS 50.0%
GGGAGGATTGAAGGGGAAAT+TGG + chr4.1:30535716-30535735 MS.gene003566:CDS 50.0%
GGGTTCATCACATGAGAACC+AGG - chr4.1:30535791-30535810 None:intergenic 50.0%
GTATGGGAGGCCAGAATTTG+GGG + chr4.1:30535930-30535949 MS.gene003566:CDS 50.0%
GTGTATGGGAGGCCAGAATT+TGG + chr4.1:30535928-30535947 MS.gene003566:CDS 50.0%
TCAGCAGCAATCTTCCCATC+TGG - chr4.1:30535854-30535873 None:intergenic 50.0%
TGGGAAGATTGCTGCTGAAG+TGG + chr4.1:30535856-30535875 MS.gene003566:CDS 50.0%
TGTCCCTGCTGAAGATGATC+AGG + chr4.1:30535457-30535476 MS.gene003566:CDS 50.0%
! CCGAATGAGGATGGATTCGA+TGG + chr4.1:30535884-30535903 MS.gene003566:CDS 50.0%
ATCGCGGGATTCACATTCGC+CGG - chr4.1:30535761-30535780 None:intergenic 55.0%
CCAGTAAGAAGCGACCGGTT+GGG + chr4.1:30535519-30535538 MS.gene003566:CDS 55.0%
GAGAGTCTCCAGTGTATGGG+AGG + chr4.1:30535917-30535936 MS.gene003566:CDS 55.0%
GATCTTCGTGGTTGTGGAGG+TGG + chr4.1:30535959-30535978 MS.gene003566:CDS 55.0%
GATGAGGAAGTTGCAAGGGC+AGG + chr4.1:30535640-30535659 MS.gene003566:CDS 55.0%
GCGACCGGTTGGGAATAAAG+AGG + chr4.1:30535529-30535548 MS.gene003566:CDS 55.0%
TCCAGTAAGAAGCGACCGGT+TGG + chr4.1:30535518-30535537 MS.gene003566:CDS 55.0%
TTGGGGCTCAAGGATCTTCG+TGG + chr4.1:30535947-30535966 MS.gene003566:CDS 55.0%
! AGGCCAGAATTTGGGGCTCA+AGG + chr4.1:30535937-30535956 MS.gene003566:CDS 55.0%
!! ACCAGGTGGGTTACACGTGT+GGG - chr4.1:30535390-30535409 None:intergenic 55.0%
ACCCACACGTGTAACCCACC+TGG + chr4.1:30535386-30535405 MS.gene003566:CDS 60.0%
CCCAACCGGTCGCTTCTTAC+TGG - chr4.1:30535522-30535541 None:intergenic 60.0%
GAAAGCTAGGGCTGCGTCGT+TGG + chr4.1:30535577-30535596 MS.gene003566:CDS 60.0%
GTTGCAAGGGCAGGCTGCTT+TGG + chr4.1:30535649-30535668 MS.gene003566:CDS 60.0%
TCGTGGTTGTGGAGGTGGAC+CGG + chr4.1:30535964-30535983 MS.gene003566:CDS 60.0%
TGAAGTGGCGTCGCCGAATG+AGG + chr4.1:30535871-30535890 MS.gene003566:CDS 60.0%
! CTTTGGAGGCCGAAGTTGCG+AGG + chr4.1:30535666-30535685 MS.gene003566:CDS 60.0%
!! GACCAGGTGGGTTACACGTG+TGG - chr4.1:30535391-30535410 None:intergenic 60.0%
AGCTAGGGCTGCGTCGTTGG+AGG + chr4.1:30535580-30535599 MS.gene003566:CDS 65.0%
GGCTGCGTCGTTGGAGGATG+AGG + chr4.1:30535586-30535605 MS.gene003566:CDS 65.0%
GGGTCTCGTGCAGCCAAAGC+CGG + chr4.1:30536854-30536873 MS.gene003566:CDS 65.0%
GTGGCGTCGCCGAATGAGGA+TGG + chr4.1:30535875-30535894 MS.gene003566:CDS 65.0%
! GCAAGGGCAGGCTGCTTTGG+AGG + chr4.1:30535652-30535671 MS.gene003566:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 30535254 30536880 30535254 ID=MS.gene003566
chr4.1 mRNA 30535254 30536880 30535254 ID=MS.gene003566.t1;Parent=MS.gene003566
chr4.1 exon 30535254 30536028 30535254 ID=MS.gene003566.t1.exon1;Parent=MS.gene003566.t1
chr4.1 CDS 30535254 30536028 30535254 ID=cds.MS.gene003566.t1;Parent=MS.gene003566.t1
chr4.1 exon 30536852 30536880 30536852 ID=MS.gene003566.t1.exon2;Parent=MS.gene003566.t1
chr4.1 CDS 30536852 30536880 30536852 ID=cds.MS.gene003566.t1;Parent=MS.gene003566.t1
Gene Sequence

>MS.gene003566

ATGGAAGACGGTGAACTTGATTTCTCAAATCAAGACGTGTTTTCGAGTCCAAACATGGGTGAATTTCAAACAAGTGGTTCAATGGATAGTTTTTTTGATGAATTGTTGAAGGATACACACGCTTGTACTCATACCCACACGTGTAACCCACCTGGTCCTGATTTTTCACACACTCACACGTGTTATCATGTTCACACCAAGATTGTCCCTGCTGAAGATGATCAGGTTGGGACTGATGATACTGCTGAATCTGCTGAGAAGAAGTCCAGTAAGAAGCGACCGGTTGGGAATAAAGAGGCCGTTAGGAAGTATCGTGAGAAGAAGAAAGCTAGGGCTGCGTCGTTGGAGGATGAGGTCATGAAATTGAGGGCTTTGAATCAGCATTTGATGAGGAAGTTGCAAGGGCAGGCTGCTTTGGAGGCCGAAGTTGCGAGGTTGAAGTGTTTGTTGGTTGATATAAGGGGGAGGATTGAAGGGGAAATTGGGTCATTTCCGTATCAGAAACCGGCGAATGTGAATCCCGCGATGAATTTGCCTGGTTCTCATGTGATGAACCCTTGTAATGTGCAGTGTGATGATCGGGCATATTGCCTTCGTCCAGATGGGAAGATTGCTGCTGAAGTGGCGTCGCCGAATGAGGATGGATTCGATGGTTGTGAGTTTGAGAGTCTCCAGTGTATGGGAGGCCAGAATTTGGGGCTCAAGGATCTTCGTGGTTGTGGAGGTGGACCGGCGTTGTCTAATGTCAATTCTCAAGCTTCCAGTAAAAGAAAAGGTGATGACTGATTGTGCACATACTCTACTAGAAAGGGTTAAATATTGTTTTTTAAAAATTAGTTCATACTTATATTTGATTTCGTTTAATTGCTGCTGCATGTTGTGTCTAGCATTTTTTGTCATGGTTTGGGATCCAATGATGATTGTTTGTCTCGTTGTTTGATTTTTGCTTTGTAAACACTATAAAACATTGAGAGTGAGAATTTAGTACAAAGAAAGAACTTCAGAGATGAAGCATCAATTTCAATAACCCTTGTTTGTTTTTGCATTATGTTTTAGTTGCTAAAATTGACTCATGTAGCTGACTCTGCCTTATATGGAAAGGCTTTTGTTGTTTTTGTTTGTTTAATTGCTAAAAATACATATCTGTTCTTATCTCTAGTAATGAAATCTGATTGCTATGTTTTATAGATGATGAATGTTACCCTTGCTCATTCCGGATTTTAAAGGGAGTCTAGTAATTATTAGTACTAGTATTACAACTTATAGAGTTCTTGTTAGTTTGTATGGACCAGATGCTTTATATAAGTATGAGTTAACAAGTGGTTCCAAATTATGACAAGATCTTCCAGTAGATTGGAGAAATTGAAATTTGAAGGTTTGAAGTCTTGAATGATCATAGTGAACATTTTTTCCCCCCTGTACTAAAAAGTTGAGACTAGATTTTCTTAATGAAAATTTGATAATATAATTTTGTATCGCTATTTCCAAAGCAAAGCAATTGTGATTGACTCCGGTTTGATAACTTTAGGGCATTGAAGTTGCTTATTCGTTCTTGCTTCTCATTCTGTAAAATGGTCTAACAGATCTTTAATTAACAGGGGGGTCTCGTGCAGCCAAAGCCGGATGA
Protein sequence

>MS.gene003566.t1

MEDGELDFSNQDVFSSPNMGEFQTSGSMDSFFDELLKDTHACTHTHTCNPPGPDFSHTHTCYHVHTKIVPAEDDQVGTDDTAESAEKKSSKKRPVGNKEAVRKYREKKKARAASLEDEVMKLRALNQHLMRKLQGQAALEAEVARLKCLLVDIRGRIEGEIGSFPYQKPANVNPAMNLPGSHVMNPCNVQCDDRAYCLRPDGKIAAEVASPNEDGFDGCEFESLQCMGGQNLGLKDLRGCGGGPALSNVNSQASSKRKGGSRAAKAG