AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003653

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003653.t1 MTR_4g071590 96.959 296 8 1 1 296 1 295 0.0 596
MS.gene003653.t1 MTR_4g471590 96.959 296 8 1 1 296 1 295 0.0 596
MS.gene003653.t1 MTR_3g117380 53.292 319 107 8 1 296 15 314 3.38e-97 289
MS.gene003653.t1 MTR_8g076340 39.159 309 138 10 1 281 1 287 1.17e-51 171
MS.gene003653.t1 MTR_5g021340 40.282 355 137 11 1 296 51 389 3.44e-50 171
MS.gene003653.t1 MTR_3g109760 38.406 276 120 9 36 281 21 276 4.55e-45 155
MS.gene003653.t1 MTR_8g013950 38.869 283 113 9 39 281 33 295 2.90e-43 151
MS.gene003653.t1 MTR_1g006650 34.921 315 141 12 15 281 5 303 1.39e-42 149
MS.gene003653.t1 MTR_1g094930 75.714 70 17 0 207 276 142 211 5.69e-35 126
MS.gene003653.t1 MTR_1g094930 75.714 70 17 0 207 276 237 306 1.71e-34 128
MS.gene003653.t1 MTR_6g032830 81.538 65 12 0 212 276 192 256 2.67e-34 125
MS.gene003653.t1 MTR_7g085410 80.000 65 13 0 212 276 188 252 2.88e-33 123
MS.gene003653.t1 MTR_1g116010 67.532 77 25 0 212 288 164 240 4.65e-33 122
MS.gene003653.t1 MTR_1g046680 76.471 68 16 0 212 279 172 239 1.05e-32 121
MS.gene003653.t1 MTR_2g094600 62.791 86 30 1 191 276 197 280 3.26e-32 121
MS.gene003653.t1 MTR_4g058910 73.134 67 18 0 212 278 314 380 1.59e-31 121
MS.gene003653.t1 MTR_5g007600 68.571 70 22 0 212 281 259 328 1.22e-29 115
MS.gene003653.t1 MTR_5g007600 59.036 83 28 1 199 275 138 220 2.68e-26 106
MS.gene003653.t1 MTR_7g446110 57.143 63 27 0 211 273 186 248 2.23e-20 88.6
MS.gene003653.t1 MTR_7g445950 30.128 156 105 1 118 273 30 181 2.52e-20 86.7
MS.gene003653.t1 MTR_7g445980 51.316 76 36 1 199 273 20 95 3.03e-19 81.3
MS.gene003653.t1 MTR_7g446070 31.868 182 107 5 105 273 16 193 6.41e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003653.t1 AT4G36240 47.082 257 90 10 40 282 10 234 1.36e-56 182
MS.gene003653.t1 AT5G66320 35.838 346 139 10 8 291 5 329 2.34e-51 172
MS.gene003653.t1 AT5G66320 35.838 346 139 10 8 291 5 329 2.34e-51 172
MS.gene003653.t1 AT4G34680 41.034 290 114 11 6 283 7 251 1.97e-49 165
MS.gene003653.t1 AT4G34680 41.034 290 114 11 6 283 7 251 1.97e-49 165
MS.gene003653.t1 AT5G25830 38.047 297 108 12 40 281 13 288 1.45e-44 154
MS.gene003653.t1 AT3G60530 47.778 180 73 5 112 283 63 229 2.27e-43 149
MS.gene003653.t1 AT4G32890 36.331 278 115 11 39 281 16 266 4.71e-43 150
MS.gene003653.t1 AT2G45050 44.845 194 73 4 112 281 65 248 4.87e-43 148
MS.gene003653.t1 AT3G51080 69.231 91 22 2 212 296 221 311 2.45e-38 137
MS.gene003653.t1 AT3G24050 64.706 85 29 1 197 280 178 262 1.02e-33 124
MS.gene003653.t1 AT3G54810 75.000 68 17 0 212 279 229 296 3.72e-33 124
MS.gene003653.t1 AT3G54810 75.000 68 17 0 212 279 229 296 3.72e-33 124
MS.gene003653.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.50e-32 121
MS.gene003653.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.50e-32 121
MS.gene003653.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.50e-32 121
MS.gene003653.t1 AT1G08000 71.014 69 20 0 212 280 218 286 2.71e-30 116
MS.gene003653.t1 AT1G08000 71.014 69 20 0 212 280 218 286 2.71e-30 116
MS.gene003653.t1 AT3G45170 60.274 73 29 0 214 286 117 189 9.39e-27 104
MS.gene003653.t1 AT2G28340 63.077 65 24 0 214 278 193 257 1.60e-24 100

Find 87 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTCTTGGTTGTTCT+AGG 0.195513 4.1:+31531296 None:intergenic
AGCCCCACCCGTTTGGGCTT+CGG 0.226880 4.1:+31531208 None:intergenic
GCCCCACCCGTTTGGGCTTC+GGG 0.232140 4.1:+31531209 None:intergenic
GGCCGATGATGTTGCGGATT+TGG 0.241470 4.1:-31531453 MS.gene003653:CDS
GAACAGCCACCGTAAGGTTT+TGG 0.270098 4.1:-31531012 MS.gene003653:CDS
GAGGTGGATGAACCGGTTTC+CGG 0.310815 4.1:-31530972 MS.gene003653:CDS
GGTTCGGCCCGGGCCTGAAC+TGG 0.312951 4.1:+31531377 None:intergenic
GAAACTGAGCCCCACCCGTT+TGG 0.331959 4.1:+31531201 None:intergenic
TCTTTGCTGAAAAGAGTATA+GGG 0.337151 4.1:+31531246 None:intergenic
TCTCTTCACAAATTGTTTGT+TGG 0.346081 4.1:+31532010 None:intergenic
AAACTGAGCCCCACCCGTTT+GGG 0.350163 4.1:+31531202 None:intergenic
CTTCTTCTTCTTCCTCCTCC+TGG 0.352825 4.1:+31531869 None:intergenic
CTCTCTATTGTATCCAGTTC+AGG 0.364580 4.1:-31531390 MS.gene003653:CDS
AACCGGTTTCCGGTCTGAAC+CGG 0.370991 4.1:-31530962 MS.gene003653:CDS
GCATTGGTTGGGCCCGGTCT+AGG 0.372639 4.1:+31531350 None:intergenic
ACAGTTGGGCCTTCTTGTCT+TGG 0.379924 4.1:+31531286 None:intergenic
GTTGGGCCCGGTCTAGGTTC+CGG 0.399228 4.1:+31531356 None:intergenic
AGTTCAGGCCCGGGCCGAAC+CGG 0.407384 4.1:-31531375 MS.gene003653:CDS
GTCTAGGTTCCGGTTCGGCC+CGG 0.414385 4.1:+31531366 None:intergenic
GGCCGAACCGGAACCTAGAC+CGG 0.414860 4.1:-31531363 MS.gene003653:CDS
TTCTTTGCTGAAAAGAGTAT+AGG 0.416988 4.1:+31531245 None:intergenic
AACGCAAACAACGTTGTTGT+CGG 0.417578 4.1:-31531976 MS.gene003653:CDS
TTTGGAAATGAGGCGGAGGA+AGG 0.432787 4.1:-31530994 MS.gene003653:CDS
TATGTAACGCTTGTGGTGTT+CGG 0.433641 4.1:-31531106 MS.gene003653:CDS
GAATTTCAACATGGTTCTGT+TGG 0.444517 4.1:-31531910 MS.gene003653:CDS
TATTGTATCCAGTTCAGGCC+CGG 0.446736 4.1:-31531385 MS.gene003653:CDS
GGAATGGGTGTCTCACTTTG+TGG 0.449579 4.1:-31531432 MS.gene003653:CDS
AATGAGGCGGAGGAAGGAGG+TGG 0.451531 4.1:-31530988 MS.gene003653:CDS
ATCCGGTTCAGACCGGAAAC+CGG 0.452815 4.1:+31530960 None:intergenic
GAGGAAGGAGGTGGATGAAC+CGG 0.452883 4.1:-31530979 MS.gene003653:CDS
TCTAGGTTCCGGTTCGGCCC+GGG 0.468440 4.1:+31531367 None:intergenic
ATAGATTCTGTCCTCAAGCT+TGG 0.468461 4.1:+31532036 None:intergenic
GCCTCATTTCCAAAACCTTA+CGG 0.469337 4.1:+31531003 None:intergenic
TTCTGCGGATTATAGAGAGC+TGG 0.472534 4.1:+31531321 None:intergenic
CGGTCTGAACCGGATTCAAA+TGG 0.475660 4.1:-31530952 MS.gene003653:CDS
ATGATGTTGCGGATTTGGAA+TGG 0.479723 4.1:-31531448 MS.gene003653:CDS
AACTCGTGACCATTTGAATC+CGG 0.481371 4.1:+31530943 None:intergenic
CAGTGGAGAGCTGGTCCGCT+TGG 0.483307 4.1:-31531140 MS.gene003653:CDS
GGAGTCAGACTCGCCGTCTC+CGG 0.485968 4.1:+31531759 None:intergenic
TGTGGTGTTCGGTTTAAGTC+CGG 0.492368 4.1:-31531095 MS.gene003653:CDS
GGATACAATAGAGAGAGTTC+TGG 0.493646 4.1:+31531398 None:intergenic
GCCCGAAGCCCAAACGGGTG+GGG 0.493923 4.1:-31531210 MS.gene003653:CDS
AAAATCCACACGTTACAGTT+GGG 0.498357 4.1:+31531272 None:intergenic
TGATGTTGCGGATTTGGAAT+GGG 0.501793 4.1:-31531447 MS.gene003653:CDS
GGAAATGAGGCGGAGGAAGG+AGG 0.505698 4.1:-31530991 MS.gene003653:CDS
GCCCGGTCTAGGTTCCGGTT+CGG 0.510484 4.1:+31531361 None:intergenic
AGACTCGCCGTCTCCGGCAC+CGG 0.510633 4.1:+31531765 None:intergenic
AGCTGGGAATAAGCATTGGT+TGG 0.510903 4.1:+31531338 None:intergenic
TTCTCAGGCCGATGATGTTG+CGG 0.519029 4.1:-31531459 MS.gene003653:intron
AAAAGAAATTGATGTTTACC+AGG 0.530934 4.1:-31531887 MS.gene003653:CDS
GCTGGGAATAAGCATTGGTT+GGG 0.531676 4.1:+31531339 None:intergenic
AAAACGCCACAGTGGAGAGC+TGG 0.532352 4.1:-31531149 MS.gene003653:CDS
AATTGATGTTTACCAGGAGG+AGG 0.533216 4.1:-31531881 MS.gene003653:CDS
CACTGGAACTGCTAAACCAC+CGG 0.535035 4.1:+31531729 None:intergenic
TGCATGCAGGTCTATACTCC+GGG 0.538197 4.1:+31531063 None:intergenic
TTCCAAATCCGCAACATCAT+CGG 0.540001 4.1:+31531451 None:intergenic
AGCCCGAAGCCCAAACGGGT+GGG 0.541657 4.1:-31531211 MS.gene003653:CDS
GAATAAGCATTGGTTGGGCC+CGG 0.542060 4.1:+31531344 None:intergenic
ATTGTATCCAGTTCAGGCCC+GGG 0.543140 4.1:-31531384 MS.gene003653:CDS
AGAGAGCTGGGAATAAGCAT+TGG 0.549150 4.1:+31531334 None:intergenic
GCCGAACCGGAACCTAGACC+GGG 0.552879 4.1:-31531362 MS.gene003653:CDS
TCTGCGGATTATAGAGAGCT+GGG 0.553907 4.1:+31531322 None:intergenic
CATTTGAATCCGGTTCAGAC+CGG 0.555998 4.1:+31530953 None:intergenic
GCTGTTCGAATGAATTTCAC+CGG 0.556294 4.1:+31531028 None:intergenic
AAGCCCGAAGCCCAAACGGG+TGG 0.557944 4.1:-31531212 MS.gene003653:CDS
TCCGGCACCGGAAAATGTTG+TGG 0.574032 4.1:+31531777 None:intergenic
TCATTCGAACAGCCACCGTA+AGG 0.576237 4.1:-31531018 MS.gene003653:CDS
CTGCATGCAGGTCTATACTC+CGG 0.578551 4.1:+31531062 None:intergenic
CTAGAACAACCAAGACAAGA+AGG 0.581886 4.1:-31531295 MS.gene003653:CDS
ACCATGTTGAAATTCACCGT+TGG 0.582797 4.1:+31531918 None:intergenic
ATACTCCGGGAAAAGCCGAC+CGG 0.594686 4.1:+31531076 None:intergenic
TGGTTGTTCTAGGCTTTCTG+CGG 0.603627 4.1:+31531306 None:intergenic
GAAAACGTCGGACTGCATGC+AGG 0.603890 4.1:+31531050 None:intergenic
AAACATGGAAGTTGTTGTAG+CGG 0.614309 4.1:-31532070 None:intergenic
TCGTTCATGTTCAGAACCTG+AGG 0.628083 4.1:+31531825 None:intergenic
TTCATGTTCAGAACCTGAGG+TGG 0.629594 4.1:+31531828 None:intergenic
GTGTTCGGTTTAAGTCCGGT+CGG 0.631814 4.1:-31531091 MS.gene003653:CDS
TCCAACGGTGAATTTCAACA+TGG 0.652718 4.1:-31531919 MS.gene003653:CDS
GAAGGCCCAACTGTAACGTG+TGG 0.654146 4.1:-31531277 MS.gene003653:CDS
AAAACACTATGTAACGCTTG+TGG 0.656575 4.1:-31531113 MS.gene003653:CDS
TGAATTTCACCGGAAAACGT+CGG 0.667076 4.1:+31531038 None:intergenic
AGAAATTGATGTTTACCAGG+AGG 0.667546 4.1:-31531884 MS.gene003653:CDS
AAGCGTTGAAACCAAGCTTG+AGG 0.681951 4.1:-31532047 MS.gene003653:CDS
TCATTTCCAAAACCTTACGG+TGG 0.687073 4.1:+31531006 None:intergenic
AGCGGACCAGCTCTCCACTG+TGG 0.697012 4.1:+31531143 None:intergenic
ATGTTCAGAAAACGCCACAG+TGG 0.715931 4.1:-31531157 MS.gene003653:CDS
GTGGGGCTCAGTTTCAACGA+CGG 0.724625 4.1:-31531193 MS.gene003653:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAAATTGATGTTTACC+AGG - chr4.1:31531120-31531139 MS.gene003653:CDS 25.0%
!!! TTTTGGTTTTCTTAATTCTC+AGG - chr4.1:31531533-31531552 MS.gene003653:intron 25.0%
AATTCATTACCTTTGTAGCA+TGG - chr4.1:31531352-31531371 MS.gene003653:CDS 30.0%
TCTCTTCACAAATTGTTTGT+TGG + chr4.1:31531000-31531019 None:intergenic 30.0%
! TCAAATTCCACAACATTTTC+CGG - chr4.1:31531223-31531242 MS.gene003653:CDS 30.0%
! TCATAGCTAATAACTCGTTT+TGG - chr4.1:31531516-31531535 MS.gene003653:intron 30.0%
! TCTTTGCTGAAAAGAGTATA+GGG + chr4.1:31531764-31531783 None:intergenic 30.0%
! TTCTTTGCTGAAAAGAGTAT+AGG + chr4.1:31531765-31531784 None:intergenic 30.0%
AAAAATCCACACGTTACAGT+TGG + chr4.1:31531739-31531758 None:intergenic 35.0%
AAAACACTATGTAACGCTTG+TGG - chr4.1:31531894-31531913 MS.gene003653:CDS 35.0%
AAAATCCACACGTTACAGTT+GGG + chr4.1:31531738-31531757 None:intergenic 35.0%
AGAAATTGATGTTTACCAGG+AGG - chr4.1:31531123-31531142 MS.gene003653:CDS 35.0%
TATCATTAGTGTCATGTGTC+CGG - chr4.1:31531464-31531483 MS.gene003653:intron 35.0%
TTGCATGATTAAAGACTCAC+TGG + chr4.1:31531298-31531317 None:intergenic 35.0%
! CAAGCGTTACATAGTGTTTT+CGG + chr4.1:31531894-31531913 None:intergenic 35.0%
! GAATTTCAACATGGTTCTGT+TGG - chr4.1:31531097-31531116 MS.gene003653:CDS 35.0%
!! GACTTGCTTGATTTTTCCAA+CGG - chr4.1:31531073-31531092 MS.gene003653:CDS 35.0%
AACGCAAACAACGTTGTTGT+CGG - chr4.1:31531031-31531050 MS.gene003653:CDS 40.0%
AACTCGTGACCATTTGAATC+CGG + chr4.1:31532067-31532086 None:intergenic 40.0%
AATTGATGTTTACCAGGAGG+AGG - chr4.1:31531126-31531145 MS.gene003653:CDS 40.0%
ACCATGTTGAAATTCACCGT+TGG + chr4.1:31531092-31531111 None:intergenic 40.0%
ATAGATTCTGTCCTCAAGCT+TGG + chr4.1:31530974-31530993 None:intergenic 40.0%
ATGATGTTGCGGATTTGGAA+TGG - chr4.1:31531559-31531578 MS.gene003653:intron 40.0%
CAGAAATGTCCATGCTACAA+AGG + chr4.1:31531364-31531383 None:intergenic 40.0%
CTAGAACAACCAAGACAAGA+AGG - chr4.1:31531712-31531731 MS.gene003653:intron 40.0%
CTCTCTATTGTATCCAGTTC+AGG - chr4.1:31531617-31531636 MS.gene003653:intron 40.0%
CTTCTTGTCTTGGTTGTTCT+AGG + chr4.1:31531714-31531733 None:intergenic 40.0%
GCCTCATTTCCAAAACCTTA+CGG + chr4.1:31532007-31532026 None:intergenic 40.0%
GCTGTTCGAATGAATTTCAC+CGG + chr4.1:31531982-31532001 None:intergenic 40.0%
GGATACAATAGAGAGAGTTC+TGG + chr4.1:31531612-31531631 None:intergenic 40.0%
TATGTAACGCTTGTGGTGTT+CGG - chr4.1:31531901-31531920 MS.gene003653:CDS 40.0%
TCATTTCCAAAACCTTACGG+TGG + chr4.1:31532004-31532023 None:intergenic 40.0%
TCCAACGGTGAATTTCAACA+TGG - chr4.1:31531088-31531107 MS.gene003653:CDS 40.0%
TGAATTTCACCGGAAAACGT+CGG + chr4.1:31531972-31531991 None:intergenic 40.0%
TTCCAAATCCGCAACATCAT+CGG + chr4.1:31531559-31531578 None:intergenic 40.0%
! TCTTTTCAGCAAAGAAGCAG+AGG - chr4.1:31531769-31531788 MS.gene003653:CDS 40.0%
! TGATGTTGCGGATTTGGAAT+GGG - chr4.1:31531560-31531579 MS.gene003653:intron 40.0%
!!! ACCGTAAGGTTTTGGAAATG+AGG - chr4.1:31532003-31532022 MS.gene003653:CDS 40.0%
!!! GTAAGGTTTTGGAAATGAGG+CGG - chr4.1:31532006-31532025 MS.gene003653:CDS 40.0%
AGAGAGCTGGGAATAAGCAT+TGG + chr4.1:31531676-31531695 None:intergenic 45.0%
ATGTTCAGAAAACGCCACAG+TGG - chr4.1:31531850-31531869 MS.gene003653:CDS 45.0%
ATTAGTGTCATGTGTCCGGT+CGG - chr4.1:31531468-31531487 MS.gene003653:intron 45.0%
CATTTGAATCCGGTTCAGAC+CGG + chr4.1:31532057-31532076 None:intergenic 45.0%
GAAAAAAACAGCACCACCTC+AGG - chr4.1:31531166-31531185 MS.gene003653:CDS 45.0%
TATTGTATCCAGTTCAGGCC+CGG - chr4.1:31531622-31531641 MS.gene003653:intron 45.0%
TCGTTCATGTTCAGAACCTG+AGG + chr4.1:31531185-31531204 None:intergenic 45.0%
TGCTAAACCACCGGCAAAAA+TGG + chr4.1:31531272-31531291 None:intergenic 45.0%
TGTGGTGTTCGGTTTAAGTC+CGG - chr4.1:31531912-31531931 MS.gene003653:CDS 45.0%
TTCATGTTCAGAACCTGAGG+TGG + chr4.1:31531182-31531201 None:intergenic 45.0%
! ACTGTGGCGTTTTCTGAACA+TGG + chr4.1:31531851-31531870 None:intergenic 45.0%
! CATAGTGTTTTCGGTCCAAG+CGG + chr4.1:31531885-31531904 None:intergenic 45.0%
! TCTGCGGATTATAGAGAGCT+GGG + chr4.1:31531688-31531707 None:intergenic 45.0%
! TGGTTGTTCTAGGCTTTCTG+CGG + chr4.1:31531704-31531723 None:intergenic 45.0%
! TTCTGCGGATTATAGAGAGC+TGG + chr4.1:31531689-31531708 None:intergenic 45.0%
!! AAGCGTTGAAACCAAGCTTG+AGG - chr4.1:31530960-31530979 MS.gene003653:CDS 45.0%
!! AGCTGGGAATAAGCATTGGT+TGG + chr4.1:31531672-31531691 None:intergenic 45.0%
!! GAGTCTGACTCCATTTTTGC+CGG - chr4.1:31531259-31531278 MS.gene003653:CDS 45.0%
!! GCTGGGAATAAGCATTGGTT+GGG + chr4.1:31531671-31531690 None:intergenic 45.0%
AAAAAGCCCGAAGCCCAAAC+GGG - chr4.1:31531792-31531811 MS.gene003653:CDS 50.0%
ACAGTTGGGCCTTCTTGTCT+TGG + chr4.1:31531724-31531743 None:intergenic 50.0%
ATTGTATCCAGTTCAGGCCC+GGG - chr4.1:31531623-31531642 MS.gene003653:intron 50.0%
CACTGGAACTGCTAAACCAC+CGG + chr4.1:31531281-31531300 None:intergenic 50.0%
CGGTCTGAACCGGATTCAAA+TGG - chr4.1:31532055-31532074 MS.gene003653:CDS 50.0%
CTGCATGCAGGTCTATACTC+CGG + chr4.1:31531948-31531967 None:intergenic 50.0%
CTTCTTCTTCTTCCTCCTCC+TGG + chr4.1:31531141-31531160 None:intergenic 50.0%
GAAAAAGCCCGAAGCCCAAA+CGG - chr4.1:31531791-31531810 MS.gene003653:CDS 50.0%
GGAATGGGTGTCTCACTTTG+TGG - chr4.1:31531575-31531594 MS.gene003653:intron 50.0%
GTGTTCGGTTTAAGTCCGGT+CGG - chr4.1:31531916-31531935 MS.gene003653:CDS 50.0%
TCATTCGAACAGCCACCGTA+AGG - chr4.1:31531989-31532008 MS.gene003653:CDS 50.0%
TGCATGCAGGTCTATACTCC+GGG + chr4.1:31531947-31531966 None:intergenic 50.0%
TTCTCAGGCCGATGATGTTG+CGG - chr4.1:31531548-31531567 MS.gene003653:intron 50.0%
TTTGGAAATGAGGCGGAGGA+AGG - chr4.1:31532013-31532032 MS.gene003653:CDS 50.0%
! GAACAGCCACCGTAAGGTTT+TGG - chr4.1:31531995-31532014 MS.gene003653:CDS 50.0%
! GCGTTTTCTGAACATGGCAG+TGG + chr4.1:31531845-31531864 None:intergenic 50.0%
! TCCACAACATTTTCCGGTGC+CGG - chr4.1:31531229-31531248 MS.gene003653:CDS 50.0%
!! GAATAAGCATTGGTTGGGCC+CGG + chr4.1:31531666-31531685 None:intergenic 50.0%
!! TCTGACTCCATTTTTGCCGG+TGG - chr4.1:31531262-31531281 MS.gene003653:CDS 50.0%
!!! AGGTTTTGGAAATGAGGCGG+AGG - chr4.1:31532009-31532028 MS.gene003653:CDS 50.0%
AAAACGCCACAGTGGAGAGC+TGG - chr4.1:31531858-31531877 MS.gene003653:CDS 55.0%
AAACTGAGCCCCACCCGTTT+GGG + chr4.1:31531808-31531827 None:intergenic 55.0%
ATACTCCGGGAAAAGCCGAC+CGG + chr4.1:31531934-31531953 None:intergenic 55.0%
ATCCGGTTCAGACCGGAAAC+CGG + chr4.1:31532050-31532069 None:intergenic 55.0%
GAAAACGTCGGACTGCATGC+AGG + chr4.1:31531960-31531979 None:intergenic 55.0%
GAAGGCCCAACTGTAACGTG+TGG - chr4.1:31531730-31531749 MS.gene003653:intron 55.0%
GACACGACACAAACACCGAC+CGG + chr4.1:31531486-31531505 None:intergenic 55.0%
GAGGAAGGAGGTGGATGAAC+CGG - chr4.1:31532028-31532047 MS.gene003653:CDS 55.0%
GGCCGATGATGTTGCGGATT+TGG - chr4.1:31531554-31531573 MS.gene003653:intron 55.0%
GTGGGGCTCAGTTTCAACGA+CGG - chr4.1:31531814-31531833 MS.gene003653:CDS 55.0%
! AACCGGTTTCCGGTCTGAAC+CGG - chr4.1:31532045-31532064 MS.gene003653:CDS 55.0%
! GAGGTGGATGAACCGGTTTC+CGG - chr4.1:31532035-31532054 MS.gene003653:CDS 55.0%
! GCATGCAGTCCGACGTTTTC+CGG - chr4.1:31531960-31531979 MS.gene003653:CDS 55.0%
! TCCGGCACCGGAAAATGTTG+TGG + chr4.1:31531233-31531252 None:intergenic 55.0%
!! ACATTTTCCGGTGCCGGAGA+CGG - chr4.1:31531235-31531254 MS.gene003653:CDS 55.0%
!! TAAGTCCGGTCGGCTTTTCC+CGG - chr4.1:31531926-31531945 MS.gene003653:CDS 55.0%
AATGAGGCGGAGGAAGGAGG+TGG - chr4.1:31532019-31532038 MS.gene003653:CDS 60.0%
GAAACTGAGCCCCACCCGTT+TGG + chr4.1:31531809-31531828 None:intergenic 60.0%
GGAAATGAGGCGGAGGAAGG+AGG - chr4.1:31532016-31532035 MS.gene003653:CDS 60.0%
AAGCCCGAAGCCCAAACGGG+TGG - chr4.1:31531795-31531814 MS.gene003653:CDS 65.0%
AGCCCCACCCGTTTGGGCTT+CGG + chr4.1:31531802-31531821 None:intergenic 65.0%
AGCCCGAAGCCCAAACGGGT+GGG - chr4.1:31531796-31531815 MS.gene003653:CDS 65.0%
AGCGGACCAGCTCTCCACTG+TGG + chr4.1:31531867-31531886 None:intergenic 65.0%
CAGTGGAGAGCTGGTCCGCT+TGG - chr4.1:31531867-31531886 MS.gene003653:CDS 65.0%
GCCCGGTCTAGGTTCCGGTT+CGG + chr4.1:31531649-31531668 None:intergenic 65.0%
GCCGAACCGGAACCTAGACC+GGG - chr4.1:31531645-31531664 MS.gene003653:intron 65.0%
GGAGTCAGACTCGCCGTCTC+CGG + chr4.1:31531251-31531270 None:intergenic 65.0%
GGCCGAACCGGAACCTAGAC+CGG - chr4.1:31531644-31531663 MS.gene003653:intron 65.0%
GTTGGGCCCGGTCTAGGTTC+CGG + chr4.1:31531654-31531673 None:intergenic 65.0%
!! GCATTGGTTGGGCCCGGTCT+AGG + chr4.1:31531660-31531679 None:intergenic 65.0%
!! GTCTAGGTTCCGGTTCGGCC+CGG + chr4.1:31531644-31531663 None:intergenic 65.0%
!! TCTAGGTTCCGGTTCGGCCC+GGG + chr4.1:31531643-31531662 None:intergenic 65.0%
AGACTCGCCGTCTCCGGCAC+CGG + chr4.1:31531245-31531264 None:intergenic 70.0%
AGTTCAGGCCCGGGCCGAAC+CGG - chr4.1:31531632-31531651 MS.gene003653:intron 70.0%
GCCCCACCCGTTTGGGCTTC+GGG + chr4.1:31531801-31531820 None:intergenic 70.0%
GCCCGAAGCCCAAACGGGTG+GGG - chr4.1:31531797-31531816 MS.gene003653:CDS 70.0%
GGTTCGGCCCGGGCCTGAAC+TGG + chr4.1:31531633-31531652 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 31530941 31532088 31530941 ID=MS.gene003653
chr4.1 mRNA 31530941 31532088 31530941 ID=MS.gene003653.t1;Parent=MS.gene003653
chr4.1 exon 31531732 31532088 31531732 ID=MS.gene003653.t1.exon1;Parent=MS.gene003653.t1
chr4.1 CDS 31531732 31532088 31531732 ID=cds.MS.gene003653.t1;Parent=MS.gene003653.t1
chr4.1 exon 31530941 31531474 31530941 ID=MS.gene003653.t1.exon2;Parent=MS.gene003653.t1
chr4.1 CDS 31530941 31531474 31530941 ID=cds.MS.gene003653.t1;Parent=MS.gene003653.t1
Gene Sequence

>MS.gene003653

ATGGAAGTTGTTGTAGCGGAAGCGTTGAAACCAAGCTTGAGGACAGAATCTATTTTCCAACAAACAATTTGTGAAGAGATTTTGTGTTTGAACGCAAACAACGTTGTTGTCGGAGAAGATTTTTCTGTCGACGACTTGCTTGATTTTTCCAACGGTGAATTTCAACATGGTTCTGTTGGAAAAGAAATTGATGTTTACCAGGAGGAGGAAGAAGAAGAAGAACATGAAAAAAACAGCACCACCTCAGGTTCTGAACATGAACGAACAGAAGATGACAACAATTCAAATTCCACAACATTTTCCGGTGCCGGAGACGGCGAGTCTGACTCCATTTTTGCCGGTGGTTTAGCAGTTCCAGTGAGTCTTTAATCATGCAATAAATTATTTTTGTTTTTTATTTAATAAAGAATGAATTCATTACCTTTGTAGCATGGACATTTCTGATAGAAGATGTGCTAGTATGCGACACATGTCACTAACACGAAACATTTGATTACATTGAATTATGTTATTTTTTTTGTATTATCATTAGTGTCATGTGTCCGGTCGGTGTTTGTGTCGTGTCTGTATGTGCTTCATAGCTAATAACTCGTTTTGGTTTTCTTAATTCTCAGGCCGATGATGTTGCGGATTTGGAATGGGTGTCTCACTTTGTGGACGATTCTGTACCAGAACTCTCTCTATTGTATCCAGTTCAGGCCCGGGCCGAACCGGAACCTAGACCGGGCCCAACCAATGCTTATTCCCAGCTCTCTATAATCCGCAGAAAGCCTAGAACAACCAAGACAAGAAGGCCCAACTGTAACGTGTGGATTTTTAACCCTATACTCTTTTCAGCAAAGAAGCAGAGGAAAAAGCCCGAAGCCCAAACGGGTGGGGCTCAGTTTCAACGACGGTGCAGCCACTGCCATGTTCAGAAAACGCCACAGTGGAGAGCTGGTCCGCTTGGACCGAAAACACTATGTAACGCTTGTGGTGTTCGGTTTAAGTCCGGTCGGCTTTTCCCGGAGTATAGACCTGCATGCAGTCCGACGTTTTCCGGTGAAATTCATTCGAACAGCCACCGTAAGGTTTTGGAAATGAGGCGGAGGAAGGAGGTGGATGAACCGGTTTCCGGTCTGAACCGGATTCAAATGGTCACGAGTTGA
Protein sequence

>MS.gene003653.t1

MEVVVAEALKPSLRTESIFQQTICEEILCLNANNVVVGEDFSVDDLLDFSNGEFQHGSVGKEIDVYQEEEEEEEHEKNSTTSGSEHERTEDDNNSNSTTFSGAGDGESDSIFAGGLAVPADDVADLEWVSHFVDDSVPELSLLYPVQARAEPEPRPGPTNAYSQLSIIRRKPRTTKTRRPNCNVWIFNPILFSAKKQRKKPEAQTGGAQFQRRCSHCHVQKTPQWRAGPLGPKTLCNACGVRFKSGRLFPEYRPACSPTFSGEIHSNSHRKVLEMRRRKEVDEPVSGLNRIQMVTS