AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003740.t1 MTR_4g070320 90.110 364 15 3 1 363 1 344 0.0 660
MS.gene003740.t1 MTR_3g116770 53.444 421 114 18 1 363 1 397 1.54e-122 359
MS.gene003740.t1 MTR_3g116770 53.318 422 114 19 1 363 1 398 1.08e-120 354
MS.gene003740.t1 MTR_7g053410 51.659 211 78 7 72 264 220 424 1.10e-52 182
MS.gene003740.t1 MTR_7g053410 51.659 211 78 7 72 264 220 424 1.94e-52 182
MS.gene003740.t1 MTR_5g048860 60.510 157 48 5 122 268 319 471 9.72e-49 172
MS.gene003740.t1 MTR_8g012290 53.005 183 71 6 79 251 205 382 3.86e-46 164
MS.gene003740.t1 MTR_8g012290 53.005 183 71 6 79 251 205 382 4.09e-46 164
MS.gene003740.t1 MTR_8g065740 54.088 159 68 3 91 248 238 392 5.50e-46 163
MS.gene003740.t1 MTR_1g017350 45.116 215 69 7 141 338 226 408 7.77e-45 159
MS.gene003740.t1 MTR_1g017350 45.755 212 72 6 141 338 226 408 1.03e-44 159
MS.gene003740.t1 MTR_1g017350 65.833 120 27 2 141 246 226 345 5.03e-44 157
MS.gene003740.t1 MTR_1g017350 65.833 120 27 2 141 246 226 345 5.37e-44 157
MS.gene003740.t1 MTR_8g062240 75.258 97 24 0 158 254 148 244 4.42e-43 151
MS.gene003740.t1 MTR_5g037250 59.701 134 42 2 117 250 79 200 1.61e-42 148
MS.gene003740.t1 MTR_7g092510 74.194 93 24 0 154 246 166 258 6.50e-42 149
MS.gene003740.t1 MTR_1g059270 62.712 118 35 3 140 253 143 255 2.52e-41 147
MS.gene003740.t1 MTR_1g059270 64.545 110 33 2 140 248 143 247 3.01e-41 145
MS.gene003740.t1 MTR_8g099880 82.143 84 14 1 158 240 142 225 1.45e-39 143
MS.gene003740.t1 MTR_3g498825 75.000 92 20 1 158 246 144 235 2.02e-39 142
MS.gene003740.t1 MTR_6g084120 43.506 154 60 4 85 238 97 223 1.97e-25 103
MS.gene003740.t1 MTR_4g131160 71.212 66 18 1 161 226 196 260 1.32e-21 95.5
MS.gene003740.t1 MTR_4g131160 71.212 66 18 1 161 226 199 263 1.43e-21 95.5
MS.gene003740.t1 MTR_1g080890 68.182 66 20 1 161 226 193 257 1.84e-20 91.7
MS.gene003740.t1 MTR_4g065870 56.923 65 28 0 161 225 355 419 5.16e-15 76.3
MS.gene003740.t1 MTR_2g098310 52.239 67 32 0 159 225 330 396 8.57e-15 75.5
MS.gene003740.t1 MTR_1g069155 52.174 69 32 1 163 231 319 386 9.63e-15 75.9
MS.gene003740.t1 MTR_4g033295 53.846 65 30 0 161 225 118 182 1.55e-14 72.0
MS.gene003740.t1 MTR_2g098310 53.030 66 31 0 159 224 330 395 2.21e-14 74.3
MS.gene003740.t1 MTR_1g084980 45.205 73 39 1 163 235 464 535 2.93e-14 74.7
MS.gene003740.t1 MTR_1g052470 48.718 78 38 1 154 229 289 366 3.07e-14 73.6
MS.gene003740.t1 MTR_4g033295 52.239 67 31 1 161 226 118 184 3.20e-14 70.9
MS.gene003740.t1 MTR_4g065870 56.452 62 27 0 161 222 355 416 3.21e-14 73.9
MS.gene003740.t1 MTR_1g018090 45.455 88 43 2 138 225 255 337 8.93e-14 72.0
MS.gene003740.t1 MTR_7g092540 45.783 83 43 1 154 234 234 316 9.42e-14 71.6
MS.gene003740.t1 MTR_1g070870 63.636 66 23 1 161 226 120 184 1.04e-13 71.2
MS.gene003740.t1 MTR_1g070870 63.636 66 23 1 161 226 123 187 1.10e-13 71.2
MS.gene003740.t1 MTR_7g099540 37.984 129 59 5 111 231 206 321 3.46e-13 70.9
MS.gene003740.t1 MTR_7g092540 47.945 73 36 1 154 224 234 306 5.42e-13 69.3
MS.gene003740.t1 MTR_1g019240 40.909 88 50 2 141 227 64 150 1.68e-12 67.4
MS.gene003740.t1 MTR_3g028520 49.231 65 33 0 161 225 322 386 3.15e-12 67.8
MS.gene003740.t1 MTR_3g027650 49.231 65 33 0 161 225 322 386 3.15e-12 67.8
MS.gene003740.t1 MTR_8g036235 47.692 65 34 0 161 225 316 380 5.97e-12 66.6
MS.gene003740.t1 MTR_7g111320 40.196 102 58 2 127 226 396 496 1.39e-11 66.2
MS.gene003740.t1 MTR_3g027650 50.000 62 31 0 161 222 322 383 1.42e-11 65.9
MS.gene003740.t1 MTR_7g099540 37.097 124 57 5 111 226 206 316 1.63e-11 65.9
MS.gene003740.t1 MTR_3g449770 36.893 103 60 3 163 262 311 411 2.72e-11 65.1
MS.gene003740.t1 MTR_5g005110 34.146 123 63 3 103 225 183 287 4.10e-11 63.9
MS.gene003740.t1 MTR_3g103030 51.562 64 30 1 162 225 230 292 4.68e-11 63.5
MS.gene003740.t1 MTR_7g110810 45.161 62 33 1 172 233 213 273 5.97e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003740.t1 AT4G34530 43.322 307 121 10 12 273 5 303 2.09e-58 192
MS.gene003740.t1 AT2G18300 59.627 161 55 5 92 251 124 275 3.71e-52 176
MS.gene003740.t1 AT1G68920 42.308 286 116 7 90 362 235 484 3.95e-52 180
MS.gene003740.t1 AT1G68920 41.497 294 123 7 82 362 228 485 5.17e-52 180
MS.gene003740.t1 AT1G68920 41.497 294 123 7 82 362 228 485 5.17e-52 180
MS.gene003740.t1 AT1G68920 41.497 294 123 7 82 362 228 485 5.17e-52 180
MS.gene003740.t1 AT2G18300 60.897 156 51 5 92 246 124 270 3.75e-51 174
MS.gene003740.t1 AT3G07340 43.617 282 120 9 81 348 195 451 9.26e-51 176
MS.gene003740.t1 AT2G18300 60.897 156 51 5 92 246 124 270 1.21e-50 173
MS.gene003740.t1 AT3G07340 53.093 194 70 4 81 274 195 367 1.55e-50 175
MS.gene003740.t1 AT3G23690 56.742 178 67 4 109 284 140 309 3.89e-49 169
MS.gene003740.t1 AT5G48560 50.000 198 93 3 57 248 192 389 5.73e-49 171
MS.gene003740.t1 AT5G48560 50.000 198 93 3 57 248 192 389 1.24e-48 171
MS.gene003740.t1 AT1G26260 57.325 157 56 1 104 260 126 271 1.49e-48 167
MS.gene003740.t1 AT1G26260 57.325 157 56 1 104 260 155 300 3.90e-48 167
MS.gene003740.t1 AT1G26260 57.325 157 56 1 104 260 177 322 5.86e-48 167
MS.gene003740.t1 AT1G26260 57.325 157 56 1 104 260 177 322 5.86e-48 167
MS.gene003740.t1 AT1G26260 57.325 157 56 1 104 260 177 322 5.86e-48 167
MS.gene003740.t1 AT1G26260 57.325 157 56 1 104 260 177 322 5.86e-48 167
MS.gene003740.t1 AT4G36540 53.254 169 58 4 92 260 93 240 2.09e-47 163
MS.gene003740.t1 AT4G36540 56.129 155 47 4 92 246 93 226 1.35e-46 161
MS.gene003740.t1 AT1G10120 45.872 218 79 5 126 343 174 352 1.41e-45 160
MS.gene003740.t1 AT1G10120 45.872 218 79 5 126 343 174 352 1.41e-45 160
MS.gene003740.t1 AT1G59640 63.636 132 41 2 127 252 97 227 7.13e-45 155
MS.gene003740.t1 AT2G42300 71.134 97 28 0 151 247 175 271 9.58e-44 152
MS.gene003740.t1 AT3G57800 63.415 123 40 2 152 274 195 312 2.80e-43 154
MS.gene003740.t1 AT1G59640 65.354 127 37 2 127 247 97 222 2.83e-43 153
MS.gene003740.t1 AT5G50915 54.777 157 53 6 112 262 85 229 5.10e-43 150
MS.gene003740.t1 AT5G50915 54.777 157 53 6 112 262 85 229 5.10e-43 150
MS.gene003740.t1 AT5G50915 54.777 157 53 6 112 262 85 229 2.13e-42 149
MS.gene003740.t1 AT5G50915 54.777 157 53 6 112 262 85 229 2.13e-42 149
MS.gene003740.t1 AT2G42300 70.833 96 28 0 151 246 175 270 2.96e-42 150
MS.gene003740.t1 AT5G62610 77.647 85 15 1 152 232 140 224 1.11e-37 137
MS.gene003740.t1 AT5G62610 77.647 85 15 1 152 232 140 224 1.11e-37 137
MS.gene003740.t1 AT1G18400 76.250 80 19 0 159 238 143 222 2.49e-35 129
MS.gene003740.t1 AT1G18400 76.250 80 19 0 159 238 143 222 4.56e-35 129
MS.gene003740.t1 AT1G73830 77.215 79 18 0 160 238 146 224 6.37e-35 129
MS.gene003740.t1 AT1G73830 77.215 79 18 0 160 238 146 224 6.66e-35 129
MS.gene003740.t1 AT3G57800 45.882 170 40 3 152 274 195 359 1.79e-34 131
MS.gene003740.t1 AT1G25330 64.286 98 34 1 152 248 94 191 2.77e-34 126
MS.gene003740.t1 AT5G58010 60.000 90 28 3 151 236 92 177 5.20e-23 97.8
MS.gene003740.t1 AT2G24260 56.471 85 36 1 142 226 16 99 2.81e-22 94.7
MS.gene003740.t1 AT4G30980 71.212 66 18 1 161 226 130 194 7.26e-22 95.1
MS.gene003740.t1 AT4G30980 71.212 66 18 1 161 226 178 242 1.11e-21 95.1
MS.gene003740.t1 AT2G24260 56.471 85 36 1 142 226 119 202 2.12e-21 94.4
MS.gene003740.t1 AT2G42300 70.000 50 15 0 151 200 175 224 3.50e-18 82.8
MS.gene003740.t1 AT2G42300 70.000 50 15 0 151 200 175 224 3.50e-18 82.8
MS.gene003740.t1 AT2G42300 70.000 50 15 0 151 200 175 224 4.06e-18 82.8
MS.gene003740.t1 AT2G20180 53.968 63 28 1 164 226 210 271 2.15e-14 74.3
MS.gene003740.t1 AT2G20180 53.968 63 28 1 164 226 210 271 2.15e-14 74.3
MS.gene003740.t1 AT2G20180 53.968 63 28 1 164 226 210 271 2.15e-14 74.3
MS.gene003740.t1 AT2G20180 53.125 64 29 1 163 226 280 342 2.18e-14 74.7
MS.gene003740.t1 AT2G20180 53.125 64 29 1 163 226 280 342 2.18e-14 74.7
MS.gene003740.t1 AT2G20180 53.125 64 29 1 163 226 280 342 2.18e-14 74.7
MS.gene003740.t1 AT2G20180 53.125 64 29 1 163 226 280 342 2.18e-14 74.7
MS.gene003740.t1 AT1G09530 46.479 71 37 1 156 226 332 401 3.74e-14 73.9
MS.gene003740.t1 AT1G09530 46.479 71 37 1 156 226 332 401 3.74e-14 73.9
MS.gene003740.t1 AT1G09530 46.479 71 37 1 156 226 332 401 3.74e-14 73.9
MS.gene003740.t1 AT1G09530 46.479 71 37 1 156 226 332 401 3.74e-14 73.9
MS.gene003740.t1 AT1G09530 46.479 71 37 1 156 226 332 401 3.74e-14 73.9
MS.gene003740.t1 AT1G09530 46.479 71 37 1 156 226 332 401 3.74e-14 73.9
MS.gene003740.t1 AT4G09180 50.746 67 33 0 159 225 182 248 6.08e-14 71.6
MS.gene003740.t1 AT2G42280 36.667 120 72 2 105 224 227 342 6.81e-14 72.4
MS.gene003740.t1 AT2G42280 37.705 122 70 3 105 224 227 344 7.52e-14 72.4
MS.gene003740.t1 AT4G28790 35.043 117 67 3 163 278 273 381 8.61e-14 72.4
MS.gene003740.t1 AT1G51140 54.688 64 29 0 161 224 304 367 9.38e-14 72.4
MS.gene003740.t1 AT4G02590 51.724 87 33 2 148 226 62 147 1.50e-13 70.1
MS.gene003740.t1 AT4G02590 51.724 87 33 2 148 226 125 210 2.33e-13 70.5
MS.gene003740.t1 AT4G02590 51.724 87 33 2 148 226 125 210 2.33e-13 70.5
MS.gene003740.t1 AT1G03040 62.121 66 24 1 161 226 139 203 2.80e-13 70.1
MS.gene003740.t1 AT4G28800 30.000 220 122 8 50 262 147 341 2.95e-13 71.2
MS.gene003740.t1 AT1G35460 50.769 65 32 0 161 225 181 245 3.10e-13 69.3
MS.gene003740.t1 AT1G03040 62.121 66 24 1 161 226 144 208 3.19e-13 70.1
MS.gene003740.t1 AT4G28815 55.172 58 25 1 164 221 71 127 3.59e-13 68.9
MS.gene003740.t1 AT2G20180 52.459 61 28 1 163 223 280 339 4.16e-13 70.1
MS.gene003740.t1 AT1G03040 62.121 66 24 1 161 226 144 208 5.63e-13 69.7
MS.gene003740.t1 AT4G28815 48.571 70 34 2 164 232 147 215 1.10e-12 68.6
MS.gene003740.t1 AT1G27740 38.938 113 68 1 113 225 119 230 1.96e-12 67.0
MS.gene003740.t1 AT4G28800 32.778 180 97 7 50 222 159 321 5.13e-12 67.4
MS.gene003740.t1 AT2G14760 39.655 116 56 4 120 225 189 300 1.10e-11 65.5
MS.gene003740.t1 AT2G46970 41.250 80 41 2 159 233 194 272 1.13e-11 65.9
MS.gene003740.t1 AT2G46970 41.250 80 41 2 159 233 216 294 1.16e-11 66.2
MS.gene003740.t1 AT2G46970 41.250 80 41 2 159 233 185 263 1.17e-11 65.9
MS.gene003740.t1 AT4G33880 43.011 93 49 3 136 225 238 329 4.17e-11 63.9
MS.gene003740.t1 AT4G00050 49.231 65 32 1 161 225 105 168 7.05e-11 62.8

Find 64 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTTTGCTCAGTGATTT+TGG 0.069577 4.1:+32576488 None:intergenic
CACTTTGATCCTCTTGTCTT+TGG 0.165749 4.1:+32576530 None:intergenic
GGGATAGTCCCTCCGGAAAT+TGG 0.186320 4.1:-32575193 MS.gene003740:CDS
TTACATTGTCTTAACACTTC+TGG 0.231856 4.1:-32576834 MS.gene003740:CDS
GACATTTCTGATTGCATCAT+TGG 0.242720 4.1:+32575289 None:intergenic
TTCCTTCGAACCCCAATTTC+CGG 0.270158 4.1:+32575181 None:intergenic
AATTTGTATCTACCTGTAAA+TGG 0.288243 4.1:+32574899 None:intergenic
TTTCCTTCTCAACCATTTAC+AGG 0.299320 4.1:-32574911 MS.gene003740:intron
TTGCATTGTCCTTACCTCTT+TGG 0.303567 4.1:+32575571 None:intergenic
GTTGGTTGTGATTCTGCTCT+TGG 0.325858 4.1:-32576651 MS.gene003740:CDS
GATGAGTCGAGAAAGATTTC+AGG 0.345790 4.1:+32575127 None:intergenic
TGGTGGATTGATAAACAATA+TGG 0.350380 4.1:-32575215 MS.gene003740:CDS
GGATAGTCCCTCCGGAAATT+GGG 0.350458 4.1:-32575192 MS.gene003740:CDS
AACAAAATTGCAGGCAAAGC+TGG 0.365496 4.1:-32575852 MS.gene003740:CDS
CAACACTGTCATGTCGGAAA+TGG 0.383436 4.1:+32576794 None:intergenic
CAATGTTGAAGTCAAGTCTT+GGG 0.403046 4.1:+32575606 None:intergenic
ATTCAATTCAGCACAACAAC+TGG 0.422469 4.1:-32575248 MS.gene003740:CDS
TGATGAATTGTTTGCCAAAG+AGG 0.429741 4.1:-32575585 MS.gene003740:intron
CCAGGATGTAACAAAATTGC+AGG 0.430070 4.1:-32575861 MS.gene003740:CDS
GCTCGAACATGAATGTAATC+AGG 0.466193 4.1:+32576372 None:intergenic
TCAATGTTGAAGTCAAGTCT+TGG 0.479932 4.1:+32575605 None:intergenic
GGTTCCAAGGAAAATTCAAA+AGG 0.493943 4.1:-32576417 MS.gene003740:CDS
TCGAAGAAACCGTTAAGAAA+AGG 0.498481 4.1:-32576581 MS.gene003740:CDS
ATCAAAGTGAGTGTTGAAGA+AGG 0.498660 4.1:-32576516 MS.gene003740:CDS
AACACTTCTGGAAATCTAGT+AGG 0.504501 4.1:-32576822 MS.gene003740:CDS
ATATCCTACCATCCTCAACT+TGG 0.507057 4.1:-32574991 MS.gene003740:CDS
AAAATCACCTTCCCAAGTTG+AGG 0.508180 4.1:+32574979 None:intergenic
CAATATGGGGATAGTCCCTC+CGG 0.510922 4.1:-32575200 MS.gene003740:CDS
TATTTGCAAGATTTAGTACC+AGG 0.510967 4.1:-32575879 MS.gene003740:CDS
GGTGGATTGATAAACAATAT+GGG 0.517497 4.1:-32575214 MS.gene003740:CDS
GAATTGAATTGAAGATATGC+AGG 0.520798 4.1:+32575262 None:intergenic
TAACTCTATGAAACCCGACC+CGG 0.524469 4.1:-32576616 MS.gene003740:CDS
TGAAGCTAGCAATCTGAAGA+TGG 0.529573 4.1:-32574576 MS.gene003740:CDS
GTCTCTTCAAAGACAAGTTG+AGG 0.543289 4.1:-32575799 MS.gene003740:intron
AGAGAAAATTGTGATGATGT+TGG 0.545309 4.1:-32576438 MS.gene003740:CDS
CTCCGGAAATTGGGGTTCGA+AGG 0.548238 4.1:-32575183 MS.gene003740:CDS
CTACCTGTAAATGGTTGAGA+AGG 0.549856 4.1:+32574908 None:intergenic
CATTTCCGACATGACAGTGT+TGG 0.552355 4.1:-32576793 MS.gene003740:CDS
CTTCCCAAGTTGAGGATGGT+AGG 0.556292 4.1:+32574987 None:intergenic
AAAATCACTGAGCAAACAAA+GGG 0.564488 4.1:-32576486 MS.gene003740:CDS
GATAGTCCCTCCGGAAATTG+GGG 0.565816 4.1:-32575191 MS.gene003740:CDS
CAACTGGTTTCATGTTGTGG+TGG 0.567785 4.1:-32575232 MS.gene003740:CDS
TTGTGATGATGTTGGTTCCA+AGG 0.568124 4.1:-32576430 MS.gene003740:CDS
TTGTGATTCTGCTCTTGGTG+AGG 0.569669 4.1:-32576646 MS.gene003740:CDS
CAACAACTGGTTTCATGTTG+TGG 0.576614 4.1:-32575235 MS.gene003740:CDS
TCAGGTAAAGATGCAGATGC+CGG 0.578456 4.1:+32575145 None:intergenic
CAAAATCACTGAGCAAACAA+AGG 0.583100 4.1:-32576487 MS.gene003740:CDS
CTACCATCCTCAACTTGGGA+AGG 0.586675 4.1:-32574986 MS.gene003740:CDS
AAGGAACAACATTAATGCAC+CGG 0.597053 4.1:-32575164 MS.gene003740:CDS
TATCCTACCATCCTCAACTT+GGG 0.602359 4.1:-32574990 MS.gene003740:CDS
TCTTTCCAACACTGTCATGT+CGG 0.609762 4.1:+32576788 None:intergenic
ACTTCTGGAAATCTAGTAGG+AGG 0.609823 4.1:-32576819 MS.gene003740:CDS
TCACCTTCCCAAGTTGAGGA+TGG 0.614865 4.1:+32574983 None:intergenic
CTTCGAACCCCAATTTCCGG+AGG 0.622595 4.1:+32575184 None:intergenic
TTCGAACCCCAATTTCCGGA+GGG 0.626102 4.1:+32575185 None:intergenic
TGTTGGAAAGACAAAGAGCA+AGG 0.628279 4.1:-32576776 MS.gene003740:CDS
AACTCTATGAAACCCGACCC+GGG 0.629719 4.1:-32576615 MS.gene003740:CDS
GAAAAGGAAAGCTGATAACA+AGG 0.638853 4.1:-32576565 MS.gene003740:CDS
AATTGAATTGAAGATATGCA+GGG 0.645477 4.1:+32575263 None:intergenic
ATTCATGTTCGAGCACGTCG+TGG 0.646984 4.1:-32576363 MS.gene003740:CDS
ATATAAAGTCCAAAGACAAG+AGG 0.657536 4.1:-32576539 MS.gene003740:CDS
GTGGATTGATAAACAATATG+GGG 0.684555 4.1:-32575213 MS.gene003740:CDS
CATGAACAAGATCAACAACA+CGG 0.698507 4.1:-32576723 MS.gene003740:CDS
AAGGAAAGCTGATAACAAGG+TGG 0.715030 4.1:-32576562 MS.gene003740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAGTTTAAGTTTATAATA+TGG - chr4.1:32575685-32575704 MS.gene003740:intron 10.0%
!!! TTTTACTAAATTGTTTAATT+TGG - chr4.1:32575303-32575322 MS.gene003740:CDS 10.0%
!! AATCTTATTAATGATTGATT+AGG + chr4.1:32576355-32576374 None:intergenic 15.0%
!! TTAAATTATTTGATCTTTAC+AGG - chr4.1:32576076-32576095 MS.gene003740:intron 15.0%
!! ATGATTGAAAGTTGAAAATT+CGG - chr4.1:32576708-32576727 MS.gene003740:CDS 20.0%
!! ATGCTAAAATTACAGTTAAT+AGG - chr4.1:32575330-32575349 MS.gene003740:intron 20.0%
!! GTATGATAAATTAGTTACTT+AGG - chr4.1:32575640-32575659 MS.gene003740:intron 20.0%
!! TAGTAAAAAATGATGTAACA+CGG - chr4.1:32575204-32575223 MS.gene003740:CDS 20.0%
!!! AAATCTTCATTTTGATGAAA+AGG + chr4.1:32576657-32576676 None:intergenic 20.0%
!!! TCTCTTAATTTGTCATTATT+TGG - chr4.1:32575133-32575152 MS.gene003740:CDS 20.0%
!!! TGATTTTTCTGTTTATTACT+CGG - chr4.1:32575435-32575454 MS.gene003740:intron 20.0%
! AATTGAATTGAAGATATGCA+GGG + chr4.1:32576145-32576164 None:intergenic 25.0%
! AATTTGTATCTACCTGTAAA+TGG + chr4.1:32576509-32576528 None:intergenic 25.0%
! CTAAAATTACAGTTAATAGG+AGG - chr4.1:32575333-32575352 MS.gene003740:intron 25.0%
!! TTTGCAAACCTTTTATATTC+TGG - chr4.1:32576741-32576760 MS.gene003740:CDS 25.0%
!! TTTTCAAAATCTTCATAGTG+TGG - chr4.1:32576445-32576464 MS.gene003740:CDS 25.0%
!!! ACACTTATTTTGTGCAATAT+AGG - chr4.1:32575401-32575420 MS.gene003740:intron 25.0%
!!! GATTAGGAACAATATTTTAG+AGG + chr4.1:32576339-32576358 None:intergenic 25.0%
!!! TTTTATATTCTGGAAAAACG+CGG - chr4.1:32576751-32576770 MS.gene003740:CDS 25.0%
AAAATCACTGAGCAAACAAA+GGG - chr4.1:32574919-32574938 MS.gene003740:CDS 30.0%
AAAGGACTTATCAATATCTG+TGG + chr4.1:32576639-32576658 None:intergenic 30.0%
AACATAAAAAACCATGATGC+TGG + chr4.1:32575899-32575918 None:intergenic 30.0%
AAGGACTTATCAATATCTGT+GGG + chr4.1:32576638-32576657 None:intergenic 30.0%
ATATAAAGTCCAAAGACAAG+AGG - chr4.1:32574866-32574885 MS.gene003740:intron 30.0%
GAATTGAATTGAAGATATGC+AGG + chr4.1:32576146-32576165 None:intergenic 30.0%
GACTCATAACGCTTTAATTA+TGG + chr4.1:32575963-32575982 None:intergenic 30.0%
GTTTATTACTCGGAGTTTAA+AGG - chr4.1:32575445-32575464 MS.gene003740:intron 30.0%
TATTTGCAAGATTTAGTACC+AGG - chr4.1:32575526-32575545 MS.gene003740:intron 30.0%
TTACATTGTCTTAACACTTC+TGG - chr4.1:32574571-32574590 MS.gene003740:CDS 30.0%
! AGAGAAAATTGTGATGATGT+TGG - chr4.1:32574967-32574986 MS.gene003740:CDS 30.0%
! ATCAGCTTTCCTTTTCTTAA+CGG + chr4.1:32574836-32574855 None:intergenic 30.0%
! GCGTTTTTCCAGAATATAAA+AGG + chr4.1:32576752-32576771 None:intergenic 30.0%
! GTGGATTGATAAACAATATG+GGG - chr4.1:32576192-32576211 MS.gene003740:intron 30.0%
! GTTCATTTTCATGTTCATGT+TGG + chr4.1:32574663-32574682 None:intergenic 30.0%
! TGAACCTTTTGAATTTTCCT+TGG + chr4.1:32574995-32575014 None:intergenic 30.0%
! TGCAAGTTGTAGAACTTTTA+AGG - chr4.1:32575852-32575871 MS.gene003740:CDS 30.0%
! TTCTTCACAACAGTTTTTGA+TGG - chr4.1:32574732-32574751 MS.gene003740:intron 30.0%
!! AGCATGCATTCTAAAACAAA+AGG + chr4.1:32576047-32576066 None:intergenic 30.0%
!! AGGAACAATATTTTAGAGGA+CGG + chr4.1:32576335-32576354 None:intergenic 30.0%
!! GGTGGATTGATAAACAATAT+GGG - chr4.1:32576191-32576210 MS.gene003740:intron 30.0%
!! TGGTGGATTGATAAACAATA+TGG - chr4.1:32576190-32576209 MS.gene003740:intron 30.0%
!!! ATGTTTTGTTTTATGACTGC+AGG - chr4.1:32576800-32576819 MS.gene003740:CDS 30.0%
!!! CTTTTAAGGTTTTCGTAGAA+AGG - chr4.1:32575866-32575885 MS.gene003740:CDS 30.0%
!!! GCATCATGGTTTTTTATGTT+AGG - chr4.1:32575899-32575918 MS.gene003740:CDS 30.0%
AAAGGTTTGCAAAGTAACCA+CGG + chr4.1:32576734-32576753 None:intergenic 35.0%
AAGGAACAACATTAATGCAC+CGG - chr4.1:32576241-32576260 MS.gene003740:intron 35.0%
AATTGTTTGCCAAAGAGGTA+AGG - chr4.1:32575825-32575844 MS.gene003740:CDS 35.0%
AGGACTTATCAATATCTGTG+GGG + chr4.1:32576637-32576656 None:intergenic 35.0%
ATTCAATTCAGCACAACAAC+TGG - chr4.1:32576157-32576176 MS.gene003740:intron 35.0%
CAAAATCACTGAGCAAACAA+AGG - chr4.1:32574918-32574937 MS.gene003740:CDS 35.0%
CAATGTTGAAGTCAAGTCTT+GGG + chr4.1:32575802-32575821 None:intergenic 35.0%
CATGAACAAGATCAACAACA+CGG - chr4.1:32574682-32574701 MS.gene003740:intron 35.0%
GAAAAGGAAAGCTGATAACA+AGG - chr4.1:32574840-32574859 MS.gene003740:intron 35.0%
GAAGGGAAAAAATAAGCGAA+AGG - chr4.1:32575497-32575516 MS.gene003740:intron 35.0%
GACATTTCTGATTGCATCAT+TGG + chr4.1:32576119-32576138 None:intergenic 35.0%
GGTTCCAAGGAAAATTCAAA+AGG - chr4.1:32574988-32575007 MS.gene003740:CDS 35.0%
TATCCATCAATCATGATCGT+CGG + chr4.1:32575243-32575262 None:intergenic 35.0%
TCAATGTTGAAGTCAAGTCT+TGG + chr4.1:32575803-32575822 None:intergenic 35.0%
TCATTGACAAGAACTGCATT+TGG + chr4.1:32575763-32575782 None:intergenic 35.0%
TCGAAGAAACCGTTAAGAAA+AGG - chr4.1:32574824-32574843 MS.gene003740:intron 35.0%
TGATGAATTGTTTGCCAAAG+AGG - chr4.1:32575820-32575839 MS.gene003740:CDS 35.0%
TTTCCTTCTCAACCATTTAC+AGG - chr4.1:32576494-32576513 MS.gene003740:CDS 35.0%
! AACACTTCTGGAAATCTAGT+AGG - chr4.1:32574583-32574602 MS.gene003740:CDS 35.0%
! CAAAAGGCGCAAATCATTAA+TGG + chr4.1:32576031-32576050 None:intergenic 35.0%
! TTGTGCAATATAGGTTAGCT+CGG - chr4.1:32575410-32575429 MS.gene003740:intron 35.0%
!! ATCAAAGTGAGTGTTGAAGA+AGG - chr4.1:32574889-32574908 MS.gene003740:intron 35.0%
!! CATAGTGTGGATTTTGATCA+AGG - chr4.1:32576458-32576477 MS.gene003740:CDS 35.0%
!! CTTTCTACTTCTAAGATTGC+AGG + chr4.1:32576562-32576581 None:intergenic 35.0%
!! CTTTGTTTGCTCAGTGATTT+TGG + chr4.1:32574920-32574939 None:intergenic 35.0%
!! TCACAACAGTTTTTGATGGT+TGG - chr4.1:32574736-32574755 MS.gene003740:intron 35.0%
AAAACGCGGTAGATTAGATG+CGG - chr4.1:32576765-32576784 MS.gene003740:CDS 40.0%
AAAATCACCTTCCCAAGTTG+AGG + chr4.1:32576429-32576448 None:intergenic 40.0%
AACAAAATTGCAGGCAAAGC+TGG - chr4.1:32575553-32575572 MS.gene003740:intron 40.0%
AAGGAAAGCTGATAACAAGG+TGG - chr4.1:32574843-32574862 MS.gene003740:intron 40.0%
ACTCATCATGTTTCACCGTA+TGG - chr4.1:32576294-32576313 MS.gene003740:intron 40.0%
ATACACAGAGAGGAACCATA+CGG + chr4.1:32576312-32576331 None:intergenic 40.0%
ATATCCTACCATCCTCAACT+TGG - chr4.1:32576414-32576433 MS.gene003740:CDS 40.0%
ATCCATCAATCATGATCGTC+GGG + chr4.1:32575242-32575261 None:intergenic 40.0%
CACTTTGATCCTCTTGTCTT+TGG + chr4.1:32574878-32574897 None:intergenic 40.0%
CCAGGATGTAACAAAATTGC+AGG - chr4.1:32575544-32575563 MS.gene003740:intron 40.0%
CTACCTGTAAATGGTTGAGA+AGG + chr4.1:32576500-32576519 None:intergenic 40.0%
GATGAGTCGAGAAAGATTTC+AGG + chr4.1:32576281-32576300 None:intergenic 40.0%
GCACAAAACAGAACAAACGT+TGG + chr4.1:32575471-32575490 None:intergenic 40.0%
GCTCGAACATGAATGTAATC+AGG + chr4.1:32575036-32575055 None:intergenic 40.0%
GTCTCTTCAAAGACAAGTTG+AGG - chr4.1:32575606-32575625 MS.gene003740:CDS 40.0%
TATCCTACCATCCTCAACTT+GGG - chr4.1:32576415-32576434 MS.gene003740:CDS 40.0%
TCTTTCCAACACTGTCATGT+CGG + chr4.1:32574620-32574639 None:intergenic 40.0%
TGAAGCTAGCAATCTGAAGA+TGG - chr4.1:32576829-32576848 MS.gene003740:CDS 40.0%
TGTTGGAAAGACAAAGAGCA+AGG - chr4.1:32574629-32574648 MS.gene003740:intron 40.0%
TTGCATTGTCCTTACCTCTT+TGG + chr4.1:32575837-32575856 None:intergenic 40.0%
! ACGTTTGTTCTGTTTTGTGC+AGG - chr4.1:32575471-32575490 MS.gene003740:intron 40.0%
! ACTTCTGGAAATCTAGTAGG+AGG - chr4.1:32574586-32574605 MS.gene003740:CDS 40.0%
! CAACAACTGGTTTCATGTTG+TGG - chr4.1:32576170-32576189 MS.gene003740:intron 40.0%
! CCTGCAATTTTGTTACATCC+TGG + chr4.1:32575547-32575566 None:intergenic 40.0%
! CTGTTTTGTGCAGGTTAGAA+GGG - chr4.1:32575480-32575499 MS.gene003740:intron 40.0%
! TCTGTTTTGTGCAGGTTAGA+AGG - chr4.1:32575479-32575498 MS.gene003740:intron 40.0%
!! ACTTCTAAGATTGCAGGCTA+AGG + chr4.1:32576556-32576575 None:intergenic 40.0%
!! TTGTGATGATGTTGGTTCCA+AGG - chr4.1:32574975-32574994 MS.gene003740:CDS 40.0%
!!! ACAACACGGACGTTACTTTT+CGG - chr4.1:32574696-32574715 MS.gene003740:intron 40.0%
!!! TCTTCGACTTTTTCGAAACC+CGG + chr4.1:32574811-32574830 None:intergenic 40.0%
AGCTGATACAGCCTTAATCG+CGG + chr4.1:32575932-32575951 None:intergenic 45.0%
ATCCCGACGATCATGATTGA+TGG - chr4.1:32575237-32575256 MS.gene003740:CDS 45.0%
CAACACTGTCATGTCGGAAA+TGG + chr4.1:32574614-32574633 None:intergenic 45.0%
CATTTCCGACATGACAGTGT+TGG - chr4.1:32574612-32574631 MS.gene003740:intron 45.0%
GAAAGTTGAAAATTCGGCCG+TGG - chr4.1:32576714-32576733 MS.gene003740:CDS 45.0%
GTTGGTTGTGATTCTGCTCT+TGG - chr4.1:32574754-32574773 MS.gene003740:intron 45.0%
TAACTCTATGAAACCCGACC+CGG - chr4.1:32574789-32574808 MS.gene003740:intron 45.0%
TCAGGTAAAGATGCAGATGC+CGG + chr4.1:32576263-32576282 None:intergenic 45.0%
TGTTGTGATTCGCGATGTTG+TGG + chr4.1:32576005-32576024 None:intergenic 45.0%
TTCCTTCGAACCCCAATTTC+CGG + chr4.1:32576227-32576246 None:intergenic 45.0%
TTGTGATTCTGCTCTTGGTG+AGG - chr4.1:32574759-32574778 MS.gene003740:intron 45.0%
! CAACTGGTTTCATGTTGTGG+TGG - chr4.1:32576173-32576192 MS.gene003740:intron 45.0%
! GGTTTTCGTAGAAAGGTTCG+CGG - chr4.1:32575873-32575892 MS.gene003740:CDS 45.0%
!!! CAACACGGACGTTACTTTTC+GGG - chr4.1:32574697-32574716 MS.gene003740:intron 45.0%
!!! CTTCGACTTTTTCGAAACCC+GGG + chr4.1:32574810-32574829 None:intergenic 45.0%
!!! GGACGTTACTTTTCGGGAAA+TGG - chr4.1:32574703-32574722 MS.gene003740:intron 45.0%
AACTCTATGAAACCCGACCC+GGG - chr4.1:32574790-32574809 MS.gene003740:intron 50.0%
ATTCATGTTCGAGCACGTCG+TGG - chr4.1:32575042-32575061 MS.gene003740:intron 50.0%
CAATATGGGGATAGTCCCTC+CGG - chr4.1:32576205-32576224 MS.gene003740:intron 50.0%
CGCGATGTTGTGGATAATCG+CGG + chr4.1:32575995-32576014 None:intergenic 50.0%
CTACCATCCTCAACTTGGGA+AGG - chr4.1:32576419-32576438 MS.gene003740:CDS 50.0%
GATAGTCCCTCCGGAAATTG+GGG - chr4.1:32576214-32576233 MS.gene003740:intron 50.0%
GGATAGTCCCTCCGGAAATT+GGG - chr4.1:32576213-32576232 MS.gene003740:intron 50.0%
TAGAGGACGGATACACAGAG+AGG + chr4.1:32576322-32576341 None:intergenic 50.0%
TTCGAACCCCAATTTCCGGA+GGG + chr4.1:32576223-32576242 None:intergenic 50.0%
! CTTCCCAAGTTGAGGATGGT+AGG + chr4.1:32576421-32576440 None:intergenic 50.0%
! TCACCTTCCCAAGTTGAGGA+TGG + chr4.1:32576425-32576444 None:intergenic 50.0%
!!! ACTTTTTCGAAACCCGGGTC+GGG + chr4.1:32574805-32574824 None:intergenic 50.0%
!!! GACTTTTTCGAAACCCGGGT+CGG + chr4.1:32574806-32574825 None:intergenic 50.0%
CTCCGGAAATTGGGGTTCGA+AGG - chr4.1:32576222-32576241 MS.gene003740:intron 55.0%
CTTCGAACCCCAATTTCCGG+AGG + chr4.1:32576224-32576243 None:intergenic 55.0%
GGGATAGTCCCTCCGGAAAT+TGG - chr4.1:32576212-32576231 MS.gene003740:intron 55.0%
TACAGCCTTAATCGCGGTCG+TGG + chr4.1:32575926-32575945 None:intergenic 55.0%
TAGGTCCACGACCGCGATTA+AGG - chr4.1:32575918-32575937 MS.gene003740:intron 55.0%
AAGGTTCGCGGCCAGCATCA+TGG - chr4.1:32575885-32575904 MS.gene003740:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 32574568 32576859 32574568 ID=MS.gene003740
chr4.1 mRNA 32574568 32576859 32574568 ID=MS.gene003740.t1;Parent=MS.gene003740
chr4.1 exon 32576329 32576859 32576329 ID=MS.gene003740.t1.exon1;Parent=MS.gene003740.t1
chr4.1 CDS 32576329 32576859 32576329 ID=cds.MS.gene003740.t1;Parent=MS.gene003740.t1
chr4.1 exon 32575800 32575934 32575800 ID=MS.gene003740.t1.exon2;Parent=MS.gene003740.t1
chr4.1 CDS 32575800 32575934 32575800 ID=cds.MS.gene003740.t1;Parent=MS.gene003740.t1
chr4.1 exon 32575586 32575657 32575586 ID=MS.gene003740.t1.exon3;Parent=MS.gene003740.t1
chr4.1 CDS 32575586 32575657 32575586 ID=cds.MS.gene003740.t1;Parent=MS.gene003740.t1
chr4.1 exon 32575117 32575329 32575117 ID=MS.gene003740.t1.exon4;Parent=MS.gene003740.t1
chr4.1 CDS 32575117 32575329 32575117 ID=cds.MS.gene003740.t1;Parent=MS.gene003740.t1
chr4.1 exon 32574912 32575014 32574912 ID=MS.gene003740.t1.exon5;Parent=MS.gene003740.t1
chr4.1 CDS 32574912 32575014 32574912 ID=cds.MS.gene003740.t1;Parent=MS.gene003740.t1
chr4.1 exon 32574568 32574605 32574568 ID=MS.gene003740.t1.exon6;Parent=MS.gene003740.t1
chr4.1 CDS 32574568 32574605 32574568 ID=cds.MS.gene003740.t1;Parent=MS.gene003740.t1
Gene Sequence

>MS.gene003740

ATGTTACATTGTCTTAACACTTCTGGAAATCTAGTAGGAGGAACCATTTCCGACATGACAGTGTTGGAAAGACAAAGAGCAAGGAAGAAGTTCCAACATGAACATGAAAATGAACATGAACAAGATCAACAACACGGACGTTACTTTTCGGGAAATGGTTTCTCTTCTTCACAACAGTTTTTGATGGTTGGTTGTGATTCTGCTCTTGGTGAGGTTGTAGCTAACTCTATGAAACCCGACCCGGGTTTCGAAAAAGTCGAAGAAACCGTTAAGAAAAGGAAAGCTGATAACAAGGTGGATATAAAGTCCAAAGACAAGAGGATCAAAGTGAGTGTTGAAGAAGGTGAATCCAAAATCACTGAGCAAACAAAGGGACAGAAGAATACAAAGTTGAAAAATAGAGAAAATTGTGATGATGTTGGTTCCAAGGAAAATTCAAAAGGTTCAGAGATTCAAAATCAAAAGCCTGATTACATTCATGTTCGAGCACGTCGTGGTCAAGCTACTGATAGTCATAGCTTAGCTGAAAGAGTGAGTATAAATTAACAATTACTTTCACTATTTTTCTCTTAATTTGTCATTATTTGGTTAATAAAAAGTGATTATCAATAAACTCATTTTACACAATTGATTTGATAGTAAAAAATGATGTAACACGGTGAAGAAGTTATCCCGACGATCATGATTGATGGATAGTGTAAAAACATGTTACGTATAACAATTAACTCATTTATATTTTACTAAATTGTTTAATTTGGTTTAATGCTAAAATTACAGTTAATAGGAGGTAGAATCTGTTTTAAAAACATAGTTTCTATAATGAAATCAAATAGACACTTATTTTGTGCAATATAGGTTAGCTCGGTATGATTTTTCTGTTTATTACTCGGAGTTTAAAGGCCAACGTTTGTTCTGTTTTGTGCAGGTTAGAAGGGAAAAAATAAGCGAAAGGATGAAGTATTTGCAAGATTTAGTACCAGGATGTAACAAAATTGCAGGCAAAGCTGGAATGCTTGATGAAATTATTAACTATGTTCAGTCTCTTCAAAGACAAGTTGAGGTTAGAGTTCTAGTATGATAAATTAGTTACTTAGGCTGCATGAATTATAGTGTTTGATTAAGTTTAAGTTTATAATATGGTTTCAATTTTAAGAATAATTTAAAGTCTATCTAACTAATTTGTACATTATCACCAAATGCAGTTCTTGTCAATGAAATTAGCAGCTGTAAACCCAAGACTTGACTTCAACATTGATGAATTGTTTGCCAAAGAGGTAAGGACAATGCAAGTTGTAGAACTTTTAAGGTTTTCGTAGAAAGGTTCGCGGCCAGCATCATGGTTTTTTATGTTAGGTCCACGACCGCGATTAAGGCTGTATCAGCTACATTTGACCATAATTAAAGCGTTATGAGTCAACATTTGACCGCGATTATCCACAACATCGCGAATCACAACACAGCCATTAATGATTTGCGCCTTTTGTTTTAGAATGCATGCTGAAGTGCTCTTAAATTATTTGATCTTTACAGGTGTTTACTCAAAATTTTCCAATGATGCAATCAGAAATGTCTAACCCTGCATATCTTCAATTCAATTCAGCACAACAACTGGTTTCATGTTGTGGTGGATTGATAAACAATATGGGGATAGTCCCTCCGGAAATTGGGGTTCGAAGGAACAACATTAATGCACCGGCATCTGCATCTTTACCTGAAATCTTTCTCGACTCATCATGTTTCACCGTATGGTTCCTCTCTGTGTATCCGTCCTCTAAAATATTGTTCCTAATCAATCATTAATAAGATTAGTCTTCTAAAATTGATCTTAATTTATGTGTATTGCAGCATATCCTACCATCCTCAACTTGGGAAGGTGATTTTCAAAATCTTCATAGTGTGGATTTTGATCAAGGAAGATCAACATCATTTCCTTCTCAACCATTTACAGGTAGATACAAATTTATGACATGTCAAATCACTTTTTGCCTTAGCCTGCAATCTTAGAAGTAGAAAGTAGAAGCAAGACAAATTGAATTTTCATATCATAGATGATAGAGACTTGAGAGCCCCACAGATATTGATAAGTCCTTTTCATCAAAATGAAGATTTTTTATTTCATTACTTTTCTTTTTATCTGCTAATGATTGAAAGTTGAAAATTCGGCCGTGGTTACTTTGCAAACCTTTTATATTCTGGAAAAACGCGGTAGATTAGATGCGGTAACACTTCTAAATGTTTTGTTTTATGACTGCAGGCATGATTGAAGCTAGCAATCTGAAGATGGAGATGTAA
Protein sequence

>MS.gene003740.t1

MLHCLNTSGNLVGGTISDMTVLERQRARKKFQHEHENEHEQDQQHGRYFSGNGFSSSQQFLMVGCDSALGEVVANSMKPDPGFEKVEETVKKRKADNKVDIKSKDKRIKVSVEEGESKITEQTKGQKNTKLKNRENCDDVGSKENSKGSEIQNQKPDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKIAGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNIDELFAKEVFTQNFPMMQSEMSNPAYLQFNSAQQLVSCCGGLINNMGIVPPEIGVRRNNINAPASASLPEIFLDSSCFTHILPSSTWEGDFQNLHSVDFDQGRSTSFPSQPFTGMIEASNLKMEM