AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003773

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003773.t1 MTR_1g042700 85.507 69 10 0 91 159 167 235 1.95e-34 128
MS.gene003773.t1 MTR_1g069735 73.810 84 21 1 91 173 172 255 2.62e-34 127
MS.gene003773.t1 MTR_7g097150 73.810 84 21 1 91 173 172 255 3.40e-34 127
MS.gene003773.t1 MTR_1g069735 73.810 84 21 1 91 173 172 255 3.80e-34 127
MS.gene003773.t1 MTR_1g069735 73.810 84 21 1 91 173 172 255 5.37e-34 127
MS.gene003773.t1 MTR_1g442790 81.159 69 13 0 91 159 162 230 1.24e-32 123
MS.gene003773.t1 MTR_1g442790 81.159 69 13 0 91 159 162 230 1.46e-32 123
MS.gene003773.t1 MTR_1g075880 75.362 69 17 0 91 159 169 237 1.87e-29 114
MS.gene003773.t1 MTR_2g040150 68.116 69 22 0 91 159 165 233 1.86e-24 100
MS.gene003773.t1 MTR_3g104700 65.217 69 24 0 91 159 146 214 1.06e-23 98.2
MS.gene003773.t1 MTR_3g104700 65.217 69 24 0 91 159 146 214 1.32e-23 98.2
MS.gene003773.t1 MTR_1g019340 60.870 69 27 0 91 159 160 228 1.93e-22 94.7
MS.gene003773.t1 MTR_1g019340 60.870 69 27 0 91 159 160 228 2.19e-22 94.4
MS.gene003773.t1 MTR_2g081100 86.486 37 5 0 11 47 520 556 5.69e-13 67.4
MS.gene003773.t1 MTR_2g081100 86.486 37 5 0 11 47 520 556 6.64e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003773.t1 AT4G14350 82.609 69 12 0 91 159 171 239 2.47e-33 125
MS.gene003773.t1 AT4G14350 82.609 69 12 0 91 159 171 239 2.47e-33 125
MS.gene003773.t1 AT4G14350 82.609 69 12 0 91 159 171 239 2.47e-33 125
MS.gene003773.t1 AT4G14350 82.609 69 12 0 91 159 171 239 2.52e-33 125
MS.gene003773.t1 AT3G23310 81.159 69 13 0 91 159 172 240 3.02e-33 125
MS.gene003773.t1 AT1G03920 69.048 84 25 1 91 173 76 159 4.71e-30 115
MS.gene003773.t1 AT1G03920 69.048 84 25 1 91 173 189 272 4.93e-30 116
MS.gene003773.t1 AT1G03920 69.048 84 25 1 91 173 189 272 4.93e-30 116
MS.gene003773.t1 AT2G20470 66.667 84 27 1 91 173 176 259 1.08e-29 115
MS.gene003773.t1 AT2G20470 66.667 84 27 1 91 173 176 259 1.46e-29 115
MS.gene003773.t1 AT2G20470 66.667 84 27 1 91 173 176 259 1.46e-29 115
MS.gene003773.t1 AT1G30640 68.116 69 22 0 91 159 172 240 1.11e-24 101
MS.gene003773.t1 AT1G30640 68.116 69 22 0 91 159 172 240 1.11e-24 101
MS.gene003773.t1 AT1G30640 68.116 69 22 0 91 159 79 147 1.50e-24 100
MS.gene003773.t1 AT2G19400 61.905 84 31 1 91 173 157 240 2.16e-23 97.4
MS.gene003773.t1 AT4G33080 65.217 69 24 0 91 159 146 214 1.07e-22 95.5
MS.gene003773.t1 AT4G33080 65.217 69 24 0 91 159 146 214 1.18e-22 95.5
MS.gene003773.t1 AT5G09890 62.319 69 26 0 91 159 154 222 1.33e-21 92.4
MS.gene003773.t1 AT5G09890 62.319 69 26 0 91 159 154 222 1.35e-21 92.4

Find 42 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAGTACATCTTGGTTAT+TGG 0.250300 4.1:-32930941 MS.gene003773:CDS
TTGTGTTCATTGGGTTCTTC+AGG 0.255365 4.1:-32931800 MS.gene003773:intron
GATTCTTAAGTTGTGTTCAT+TGG 0.290116 4.1:-32931810 MS.gene003773:CDS
GGTGGTGACCACATGGATTC+AGG 0.293493 4.1:+32933204 None:intergenic
TGAACACAACTTAAGAATCT+TGG 0.356988 4.1:+32931814 None:intergenic
GATGAAGCTAAGTTGTATAT+CGG 0.363602 4.1:-32931896 MS.gene003773:CDS
ATGTTTACGAATAGACTCTA+TGG 0.366298 4.1:+32931859 None:intergenic
CTTATCTCTTTATTCCGTAC+AGG 0.373870 4.1:-32933640 MS.gene003773:CDS
CCTTTCCGACCTCTGGTGCT+TGG 0.384030 4.1:+32933531 None:intergenic
ATTCTTAAGTTGTGTTCATT+GGG 0.398961 4.1:-32931809 MS.gene003773:CDS
TATGGAGCAGAACCATCCTT+TGG 0.428138 4.1:+32930878 None:intergenic
ATGAAGCTAAGTTGTATATC+GGG 0.428346 4.1:-32931895 MS.gene003773:CDS
GAACACAACTTAAGAATCTT+GGG 0.431347 4.1:+32931815 None:intergenic
TGGAGAAATTTCTTTCCCTA+TGG 0.450330 4.1:-32933170 MS.gene003773:intron
CGAGTATTTATATCTCATAA+TGG 0.455404 4.1:-32931978 MS.gene003773:CDS
TCATCATCGCTGAAGGAAAC+CGG 0.458878 4.1:+32933582 None:intergenic
CAACGTCATCATATCTCCAC+TGG 0.470628 4.1:+32931946 None:intergenic
TACAGCTAGAGCTGATAGTC+CGG 0.496465 4.1:-32933601 MS.gene003773:CDS
TTCTATCAACTTCTGCAAGA+AGG 0.496737 4.1:+32932048 None:intergenic
GTCACCACCAGTGTTGGTTG+TGG 0.497369 4.1:-32933190 MS.gene003773:CDS
GTGGTGACCACATGGATTCA+GGG 0.502810 4.1:+32933205 None:intergenic
AAGAGATAAGCTAAGAATGA+AGG 0.511539 4.1:+32933653 None:intergenic
TTATCTCTTTATTCCGTACA+GGG 0.516748 4.1:-32933639 MS.gene003773:CDS
TAAATACTCGTCATCTTGAA+AGG 0.518616 4.1:+32931991 None:intergenic
GTCGCTGCTGCGGAATATCA+AGG 0.544183 4.1:-32930913 MS.gene003773:CDS
CAACACTGGTGGTGACCACA+TGG 0.551919 4.1:+32933197 None:intergenic
GGCATCTTCATCATCGCTGA+AGG 0.569407 4.1:+32933575 None:intergenic
TCGGCCTTGAAAACCCTGTA+CGG 0.580392 4.1:+32933626 None:intergenic
AATTTCTCCACAACCAACAC+TGG 0.581758 4.1:+32933183 None:intergenic
TTGGATATGTCAAATCTGAA+AGG 0.592968 4.1:-32932073 MS.gene003773:CDS
TATCAGCTCTAGCTGTACTT+CGG 0.599995 4.1:+32933607 None:intergenic
AGCTCAAAACCAAGCACCAG+AGG 0.612912 4.1:-32933540 MS.gene003773:intron
ATATGATGACGTTGCTGATG+CGG 0.617376 4.1:-32931937 MS.gene003773:CDS
CTTATGAGTCATCCAAAGGA+TGG 0.622364 4.1:-32930890 MS.gene003773:CDS
ATTGGACATAGTCGCTGCTG+CGG 0.624668 4.1:-32930923 MS.gene003773:CDS
AAGGCTTATGAGTCATCCAA+AGG 0.646224 4.1:-32930894 MS.gene003773:CDS
TTCTCCACAACCAACACTGG+TGG 0.646868 4.1:+32933186 None:intergenic
AACGTCATCATATCTCCACT+GGG 0.651072 4.1:+32931947 None:intergenic
CATGTGGTCACCACCAGTGT+TGG 0.651332 4.1:-32933196 MS.gene003773:CDS
TGAAGCTAAGTTGTATATCG+GGG 0.667273 4.1:-32931894 MS.gene003773:CDS
ATAATGGAGTATCTACCCAG+TGG 0.705024 4.1:-32931962 MS.gene003773:CDS
ATAAAATCCCTGAATCCATG+TGG 0.711354 4.1:-32933212 MS.gene003773:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATAATTACATTTTTC+AGG - chr4.1:32933571-32933590 MS.gene003773:CDS 10.0%
!! AAAATATTAGGAAAATTCAT+TGG - chr4.1:32931703-32931722 MS.gene003773:intron 15.0%
!! GATGAAATTGTAAAAATATT+AGG - chr4.1:32931691-32931710 MS.gene003773:intron 15.0%
!! GTAATTTGATGAAATATTTA+TGG - chr4.1:32931513-32931532 MS.gene003773:intron 15.0%
!!! AATAAAATTAAAGTACCATA+GGG + chr4.1:32931382-32931401 None:intergenic 15.0%
!!! TAATAAAATTAAAGTACCAT+AGG + chr4.1:32931383-32931402 None:intergenic 15.0%
!!! TTTTTTCTTTTAGAAAGATA+TGG + chr4.1:32933556-32933575 None:intergenic 15.0%
!! ACATTCAAATTTAACAAACA+TGG + chr4.1:32931561-32931580 None:intergenic 20.0%
!! AGTAATTGTAAAGTTCAATA+TGG + chr4.1:32931459-32931478 None:intergenic 20.0%
!! GAATTTGTAATCCTAATTTA+AGG + chr4.1:32931078-32931097 None:intergenic 20.0%
!!! AAACATGGTATATATGTTTT+TGG + chr4.1:32931546-32931565 None:intergenic 20.0%
!!! AAATAATTACATTTTTCAGG+TGG - chr4.1:32933574-32933593 MS.gene003773:CDS 20.0%
!!! AGTTATTGAACTTATTTTCT+TGG - chr4.1:32932176-32932195 MS.gene003773:intron 20.0%
!!! GTCATATACAATTTGTTATT+AGG - chr4.1:32931642-32931661 MS.gene003773:intron 20.0%
!!! GTTAATTTATTTTGACCATT+TGG - chr4.1:32931845-32931864 MS.gene003773:CDS 20.0%
! AAATATGTTGGAAACAGTTT+GGG - chr4.1:32931784-32931803 MS.gene003773:intron 25.0%
! AGGTAGACATTAGAAAAAAA+TGG - chr4.1:32931662-32931681 MS.gene003773:intron 25.0%
! ATATTCATAAGCTATACTTG+AGG + chr4.1:32932038-32932057 None:intergenic 25.0%
! ATTGCGAAAGAGTTAAATAA+TGG - chr4.1:32932292-32932311 MS.gene003773:intron 25.0%
! CGAGTATTTATATCTCATAA+TGG - chr4.1:32932556-32932575 MS.gene003773:intron 25.0%
! CTATACTTATACCATTGAAA+AGG + chr4.1:32932901-32932920 None:intergenic 25.0%
! CTATATATTGAGTTTGATCA+AGG + chr4.1:32931754-32931773 None:intergenic 25.0%
! GAACTTTACAATTACTATCT+AGG - chr4.1:32931463-32931482 MS.gene003773:intron 25.0%
! TAATTAGGAGATTACAGATT+CGG + chr4.1:32933051-32933070 None:intergenic 25.0%
! TGATGAAATATTTATGGCAT+GGG - chr4.1:32931519-32931538 MS.gene003773:intron 25.0%
! TTGATGAAATATTTATGGCA+TGG - chr4.1:32931518-32931537 MS.gene003773:intron 25.0%
!! ATTCTTAAGTTGTGTTCATT+GGG - chr4.1:32932725-32932744 MS.gene003773:intron 25.0%
!!! ATAGCTAAGCCATTTTTTAA+AGG - chr4.1:32932917-32932936 MS.gene003773:intron 25.0%
!!! TATTGAACTTATTTTCTTGG+TGG - chr4.1:32932179-32932198 MS.gene003773:intron 25.0%
!!! TTATTTTGACCATTTGGTAA+TGG - chr4.1:32931851-32931870 MS.gene003773:CDS 25.0%
AAATTACCCTATAAGAGTCA+AGG + chr4.1:32931500-32931519 None:intergenic 30.0%
AAGAGATAAGCTAAGAATGA+AGG + chr4.1:32930884-32930903 None:intergenic 30.0%
AATATGTTGGAAACAGTTTG+GGG - chr4.1:32931785-32931804 MS.gene003773:intron 30.0%
AATTAGGAGATTACAGATTC+GGG + chr4.1:32933050-32933069 None:intergenic 30.0%
ACTGTTTCCAACATATTTGT+CGG + chr4.1:32931782-32931801 None:intergenic 30.0%
AGAAAATCCGACAAATATGT+TGG - chr4.1:32931772-32931791 MS.gene003773:intron 30.0%
ATGAAGCTAAGTTGTATATC+GGG - chr4.1:32932639-32932658 MS.gene003773:intron 30.0%
ATGTTTACGAATAGACTCTA+TGG + chr4.1:32932678-32932697 None:intergenic 30.0%
CAAAGTAACCCAGAAAAATT+TGG + chr4.1:32931123-32931142 None:intergenic 30.0%
CAAATATGTTGGAAACAGTT+TGG - chr4.1:32931783-32931802 MS.gene003773:intron 30.0%
CTTATTTCAATAAGCTCTCT+AGG - chr4.1:32932067-32932086 MS.gene003773:CDS 30.0%
GAACACAACTTAAGAATCTT+GGG + chr4.1:32932722-32932741 None:intergenic 30.0%
GATGAAATATTTATGGCATG+GGG - chr4.1:32931520-32931539 MS.gene003773:intron 30.0%
GATGAAGCTAAGTTGTATAT+CGG - chr4.1:32932638-32932657 MS.gene003773:intron 30.0%
GGGGAAATAAACCTTAAATT+AGG - chr4.1:32931064-32931083 MS.gene003773:intron 30.0%
GGGTTATATGTTGTGTTTAA+GGG - chr4.1:32931888-32931907 MS.gene003773:CDS 30.0%
TAAATACTCGTCATCTTGAA+AGG + chr4.1:32932546-32932565 None:intergenic 30.0%
TATCTTTCACATTACAGACA+AGG + chr4.1:32933015-32933034 None:intergenic 30.0%
TCATACAATGTGCATTGAAT+AGG + chr4.1:32932207-32932226 None:intergenic 30.0%
TCTGTAATCTCCTAATTACA+TGG - chr4.1:32933053-32933072 MS.gene003773:intron 30.0%
TCTGTTACTATTATGTCAAG+AGG - chr4.1:32933356-32933375 MS.gene003773:intron 30.0%
TGAACACAACTTAAGAATCT+TGG + chr4.1:32932723-32932742 None:intergenic 30.0%
TGTTACATTGTTTGTGTGTT+CGG - chr4.1:32933089-32933108 MS.gene003773:intron 30.0%
TTATCTCTTTATTCCGTACA+GGG - chr4.1:32930895-32930914 MS.gene003773:CDS 30.0%
TTGGATATGTCAAATCTGAA+AGG - chr4.1:32932461-32932480 MS.gene003773:intron 30.0%
! AAACAGACTATTCTTTTGCA+TGG + chr4.1:32932155-32932174 None:intergenic 30.0%
! AGAAAGAGTATGTTAGTAGA+AGG + chr4.1:32933225-32933244 None:intergenic 30.0%
! AGTCTTTCTTTCCTTTTCAA+TGG - chr4.1:32932887-32932906 MS.gene003773:intron 30.0%
! GATTCTTAAGTTGTGTTCAT+TGG - chr4.1:32932724-32932743 MS.gene003773:intron 30.0%
! TCTTAATGTGTCATTAACTG+TGG + chr4.1:32933495-32933514 None:intergenic 30.0%
! TTCAAGAAACTTTTCCATTG+TGG + chr4.1:32931187-32931206 None:intergenic 30.0%
!! AGGTGCTTGTGTTTTTAATT+AGG - chr4.1:32932438-32932457 MS.gene003773:intron 30.0%
!! GCTTGTGTTTTTAATTAGGT+TGG - chr4.1:32932442-32932461 MS.gene003773:intron 30.0%
!! TAACTTTGAAACTGTATCAG+AGG + chr4.1:32932795-32932814 None:intergenic 30.0%
AAGTAGACCTGATTAAATGC+AGG + chr4.1:32933401-32933420 None:intergenic 35.0%
AATCTGCTAAGATGAATGTC+TGG + chr4.1:32933336-32933355 None:intergenic 35.0%
AGCATTTCTATTGTTAGCGA+AGG + chr4.1:32931261-32931280 None:intergenic 35.0%
AGTAGACCTGATTAAATGCA+GGG + chr4.1:32933400-32933419 None:intergenic 35.0%
ATAAAATCCCTGAATCCATG+TGG - chr4.1:32931322-32931341 MS.gene003773:intron 35.0%
ATATTCCACTCCTTGATCAA+CGG + chr4.1:32932846-32932865 None:intergenic 35.0%
CACACAAACAATGTAACAGA+TGG + chr4.1:32933087-32933106 None:intergenic 35.0%
CATCATTGACAGAAACATCT+TGG - chr4.1:32931097-32931116 MS.gene003773:intron 35.0%
CGAAGGAAATAATGACAACA+GGG + chr4.1:32931244-32931263 None:intergenic 35.0%
CTTATCTCTTTATTCCGTAC+AGG - chr4.1:32930894-32930913 MS.gene003773:CDS 35.0%
GGAGATGAAAAACTTTGATG+TGG - chr4.1:32931620-32931639 MS.gene003773:intron 35.0%
GGGAAAGACAACTCAAAAAA+TGG + chr4.1:32932825-32932844 None:intergenic 35.0%
GGGGTTATATGTTGTGTTTA+AGG - chr4.1:32931887-32931906 MS.gene003773:CDS 35.0%
GTAGTCTACAATAGACAAGA+CGG - chr4.1:32932984-32933003 MS.gene003773:intron 35.0%
GTGTAAACAATGAACCTGAT+AGG + chr4.1:32931146-32931165 None:intergenic 35.0%
GTTGTGTTTAAGGGCTTATT+TGG - chr4.1:32931897-32931916 MS.gene003773:CDS 35.0%
GTTTAAGGGCTTATTTGGAT+TGG - chr4.1:32931902-32931921 MS.gene003773:CDS 35.0%
TAGGGAAATGAGTTAGAAAC+AGG + chr4.1:32932011-32932030 None:intergenic 35.0%
TCCTGATTGCAAAGAGATAT+GGG - chr4.1:32931599-32931618 MS.gene003773:intron 35.0%
TGAAGCTAAGTTGTATATCG+GGG - chr4.1:32932640-32932659 MS.gene003773:intron 35.0%
TGGAGAAATTTCTTTCCCTA+TGG - chr4.1:32931364-32931383 MS.gene003773:intron 35.0%
TTCTATCAACTTCTGCAAGA+AGG + chr4.1:32932489-32932508 None:intergenic 35.0%
! ACATCAGATTGATAGTCATC+TGG - chr4.1:32933126-32933145 MS.gene003773:intron 35.0%
! ACATCTTGGCCAAATTTTTC+TGG - chr4.1:32931111-32931130 MS.gene003773:intron 35.0%
! AGGGATTTTATCTAGCAAGT+TGG + chr4.1:32931313-32931332 None:intergenic 35.0%
! CACAGGTTGCCTTTAAAAAA+TGG + chr4.1:32932929-32932948 None:intergenic 35.0%
! CATCAGATTGATAGTCATCT+GGG - chr4.1:32933127-32933146 MS.gene003773:intron 35.0%
! CATCTTGGCCAAATTTTTCT+GGG - chr4.1:32931112-32931131 MS.gene003773:intron 35.0%
!! AAGCTTTTTCCTGCTCAATT+AGG - chr4.1:32931402-32931421 MS.gene003773:intron 35.0%
!! GAAAGAAATGTGTTTTTGCC+TGG - chr4.1:32931725-32931744 MS.gene003773:intron 35.0%
!! TTTGACCATTTGGTAATGGA+TGG - chr4.1:32931855-32931874 MS.gene003773:CDS 35.0%
!!! AGGTTTTTCTGGTTTCAGTT+TGG + chr4.1:32931217-32931236 None:intergenic 35.0%
!!! CTTTGGTTTTGTAGATTGCT+TGG - chr4.1:32933180-32933199 MS.gene003773:CDS 35.0%
!!! TTGTTTTTGCATCGACACTT+TGG - chr4.1:32933163-32933182 MS.gene003773:intron 35.0%
!!! TTTTTCTGGTTTCAGTTTGG+TGG + chr4.1:32931214-32931233 None:intergenic 35.0%
AACATACTCTTTCTTGCACC+AGG - chr4.1:32933231-32933250 MS.gene003773:CDS 40.0%
AACGTCATCATATCTCCACT+GGG + chr4.1:32932590-32932609 None:intergenic 40.0%
AAGCTATACTTGAGGAGCTT+AGG + chr4.1:32932030-32932049 None:intergenic 40.0%
AAGGCTTATGAGTCATCCAA+AGG - chr4.1:32933640-32933659 MS.gene003773:CDS 40.0%
AATAATGACAACAGGGTTGG+AGG + chr4.1:32931237-32931256 None:intergenic 40.0%
AATTTCTCCACAACCAACAC+TGG + chr4.1:32931354-32931373 None:intergenic 40.0%
ACACGAACCTTGACTCTTAT+AGG - chr4.1:32931490-32931509 MS.gene003773:intron 40.0%
AGAAGCAAACCTAATTGAGC+AGG + chr4.1:32931414-32931433 None:intergenic 40.0%
AGCATCTCTGACTTAACTCA+CGG + chr4.1:32932353-32932372 None:intergenic 40.0%
AGCTATACTTGAGGAGCTTA+GGG + chr4.1:32932029-32932048 None:intergenic 40.0%
CACGAACCTTGACTCTTATA+GGG - chr4.1:32931491-32931510 MS.gene003773:intron 40.0%
CTTATGAGTCATCCAAAGGA+TGG - chr4.1:32933644-32933663 MS.gene003773:CDS 40.0%
GCGAAGGAAATAATGACAAC+AGG + chr4.1:32931245-32931264 None:intergenic 40.0%
GGAAATAATGACAACAGGGT+TGG + chr4.1:32931240-32931259 None:intergenic 40.0%
GTACAACCCTGCATTTAATC+AGG - chr4.1:32933391-32933410 MS.gene003773:intron 40.0%
GTCCTGATTGCAAAGAGATA+TGG - chr4.1:32931598-32931617 MS.gene003773:intron 40.0%
GTGGAGTACATCTTGGTTAT+TGG - chr4.1:32933593-32933612 MS.gene003773:CDS 40.0%
TATTCCACTCCTTGATCAAC+GGG + chr4.1:32932845-32932864 None:intergenic 40.0%
TCCCATATCTCTTTGCAATC+AGG + chr4.1:32931603-32931622 None:intergenic 40.0%
TCCTTCCATCCATTACCAAA+TGG + chr4.1:32931863-32931882 None:intergenic 40.0%
TCTTTCGCAATGTGAAACTC+CGG + chr4.1:32932283-32932302 None:intergenic 40.0%
TTGTGTTCATTGGGTTCTTC+AGG - chr4.1:32932734-32932753 MS.gene003773:intron 40.0%
! ATAATGGAGTATCTACCCAG+TGG - chr4.1:32932572-32932591 MS.gene003773:intron 40.0%
! ATATGATGACGTTGCTGATG+CGG - chr4.1:32932597-32932616 MS.gene003773:intron 40.0%
! TAATGGATGGAAGGAGAATG+GGG - chr4.1:32931868-32931887 MS.gene003773:CDS 40.0%
! TATCAGCTCTAGCTGTACTT+CGG + chr4.1:32930930-32930949 None:intergenic 40.0%
! TATTCCGTACAGGGTTTTCA+AGG - chr4.1:32930904-32930923 MS.gene003773:CDS 40.0%
! TCTGAGATAAGAACCATCGA+CGG + chr4.1:32933307-32933326 None:intergenic 40.0%
! TTTTGAGCTTGATGTTGGCA+TGG + chr4.1:32930983-32931002 None:intergenic 40.0%
!! AAAACAACGCTTTAGAGCAC+AGG + chr4.1:32932946-32932965 None:intergenic 40.0%
!! ACCATTTGGTAATGGATGGA+AGG - chr4.1:32931859-32931878 MS.gene003773:CDS 40.0%
!! GTAATGGATGGAAGGAGAAT+GGG - chr4.1:32931867-32931886 MS.gene003773:CDS 40.0%
!!! CTTGGTTTTGAGCTTGATGT+TGG + chr4.1:32930988-32931007 None:intergenic 40.0%
!!! GAAATGCTATTTTCCTCTGC+AGG - chr4.1:32931273-32931292 MS.gene003773:intron 40.0%
!!! TTTTCAGGTGGAGTACATCT+TGG - chr4.1:32933586-32933605 MS.gene003773:CDS 40.0%
AACAGTTTGGGGATAACTGC+AGG - chr4.1:32931796-32931815 MS.gene003773:intron 45.0%
AAGTCAGAGATGCTTCGTAG+AGG - chr4.1:32932359-32932378 MS.gene003773:intron 45.0%
ACAGTTTGGGGATAACTGCA+GGG - chr4.1:32931797-32931816 MS.gene003773:intron 45.0%
AGACTACAACGCATGCACAA+TGG + chr4.1:32932971-32932990 None:intergenic 45.0%
AGATACCCGTGGACATTTGT+TGG - chr4.1:32933258-32933277 MS.gene003773:intron 45.0%
AGGCAACCAACAAATGTCCA+CGG + chr4.1:32933267-32933286 None:intergenic 45.0%
CAAACTACAACCAGAGGAAG+AGG - chr4.1:32931039-32931058 MS.gene003773:intron 45.0%
CAACGTCATCATATCTCCAC+TGG + chr4.1:32932591-32932610 None:intergenic 45.0%
GACTACAACGCATGCACAAT+GGG + chr4.1:32932970-32932989 None:intergenic 45.0%
GCATCTCAAACTACAACCAG+AGG - chr4.1:32931033-32931052 MS.gene003773:intron 45.0%
GCTTCGTAGAGGACATGTAT+TGG - chr4.1:32932370-32932389 MS.gene003773:intron 45.0%
GGAGTTGCTGCCATGTAATT+AGG + chr4.1:32933066-32933085 None:intergenic 45.0%
GTATGGTTTATGACCGTCGA+TGG - chr4.1:32933291-32933310 MS.gene003773:intron 45.0%
GTCATCTATGTTTCCTGCAG+AGG + chr4.1:32931289-32931308 None:intergenic 45.0%
GTTGTCTTTCCCGTTGATCA+AGG - chr4.1:32932833-32932852 MS.gene003773:intron 45.0%
TACAACCAGAGGAAGAGGAA+GGG - chr4.1:32931044-32931063 MS.gene003773:intron 45.0%
TACAGCTAGAGCTGATAGTC+CGG - chr4.1:32930933-32930952 MS.gene003773:CDS 45.0%
TATGGAGCAGAACCATCCTT+TGG + chr4.1:32933659-32933678 None:intergenic 45.0%
TCATCATCGCTGAAGGAAAC+CGG + chr4.1:32930955-32930974 None:intergenic 45.0%
! AACAGGGTTGGAGGTTTTTC+TGG + chr4.1:32931228-32931247 None:intergenic 45.0%
! AGCTGTCTTTCTTTTCCCTC+CGG - chr4.1:32932261-32932280 MS.gene003773:intron 45.0%
! GACATTTGTTGGTTGCCTTG+GGG - chr4.1:32933269-32933288 MS.gene003773:intron 45.0%
! GGACATTTGTTGGTTGCCTT+GGG - chr4.1:32933268-32933287 MS.gene003773:intron 45.0%
! TCTGGGTTACTTTGCCTATC+AGG - chr4.1:32931129-32931148 MS.gene003773:intron 45.0%
! TGAGTTTGATCAAGGCATCC+AGG + chr4.1:32931746-32931765 None:intergenic 45.0%
! TGGACATTTGTTGGTTGCCT+TGG - chr4.1:32933267-32933286 MS.gene003773:intron 45.0%
! TTGAGTCGATTCAGCCACAA+TGG - chr4.1:32931170-32931189 MS.gene003773:intron 45.0%
!! GGTAATGGATGGAAGGAGAA+TGG - chr4.1:32931866-32931885 MS.gene003773:CDS 45.0%
!!! TATTATTTTCTTTTAATTAT+AGG - chr4.1:32932418-32932437 MS.gene003773:intron 5.0%
AACAGATCCTTTCCGACCTC+TGG + chr4.1:32931013-32931032 None:intergenic 50.0%
ACAACCAGAGGAAGAGGAAG+GGG - chr4.1:32931045-32931064 MS.gene003773:intron 50.0%
AGCTCAAAACCAAGCACCAG+AGG - chr4.1:32930994-32931013 MS.gene003773:intron 50.0%
ATTGGACATAGTCGCTGCTG+CGG - chr4.1:32933611-32933630 MS.gene003773:CDS 50.0%
ATTTCCCCTTCCTCTTCCTC+TGG + chr4.1:32931052-32931071 None:intergenic 50.0%
CAAAACCAAGCACCAGAGGT+CGG - chr4.1:32930998-32931017 MS.gene003773:intron 50.0%
CGGTCATAAACCATACCCCA+AGG + chr4.1:32933287-32933306 None:intergenic 50.0%
CTACAACCAGAGGAAGAGGA+AGG - chr4.1:32931043-32931062 MS.gene003773:intron 50.0%
CTTTCCCGTTGATCAAGGAG+TGG - chr4.1:32932838-32932857 MS.gene003773:intron 50.0%
GGCAACCAACAAATGTCCAC+GGG + chr4.1:32933266-32933285 None:intergenic 50.0%
GGCATCTTCATCATCGCTGA+AGG + chr4.1:32930962-32930981 None:intergenic 50.0%
GTGGTGACCACATGGATTCA+GGG + chr4.1:32931332-32931351 None:intergenic 50.0%
TCGCAATGTGAAACTCCGGA+GGG + chr4.1:32932279-32932298 None:intergenic 50.0%
TCGGCCTTGAAAACCCTGTA+CGG + chr4.1:32930911-32930930 None:intergenic 50.0%
TTCGCAATGTGAAACTCCGG+AGG + chr4.1:32932280-32932299 None:intergenic 50.0%
TTCTCCACAACCAACACTGG+TGG + chr4.1:32931351-32931370 None:intergenic 50.0%
! TTGTTGGTTGCCTTGGGGTA+TGG - chr4.1:32933274-32933293 MS.gene003773:intron 50.0%
CACCAGGTGTTAGATACCCG+TGG - chr4.1:32933247-32933266 MS.gene003773:intron 55.0%
CATGTGGTCACCACCAGTGT+TGG - chr4.1:32931338-32931357 MS.gene003773:intron 55.0%
GGTGGTGACCACATGGATTC+AGG + chr4.1:32931333-32931352 None:intergenic 55.0%
GTCCACGGGTATCTAACACC+TGG + chr4.1:32933252-32933271 None:intergenic 55.0%
GTCGCTGCTGCGGAATATCA+AGG - chr4.1:32933621-32933640 MS.gene003773:CDS 55.0%
! CAACACTGGTGGTGACCACA+TGG + chr4.1:32931340-32931359 None:intergenic 55.0%
!! GTCACCACCAGTGTTGGTTG+TGG - chr4.1:32931344-32931363 MS.gene003773:intron 55.0%
CCAAGCACCAGAGGTCGGAA+AGG - chr4.1:32931003-32931022 MS.gene003773:intron 60.0%
CCTTTCCGACCTCTGGTGCT+TGG + chr4.1:32931006-32931025 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 32930877 32933679 32930877 ID=MS.gene003773
chr4.1 mRNA 32930877 32933679 32930877 ID=MS.gene003773.t1;Parent=MS.gene003773
chr4.1 exon 32933541 32933679 32933541 ID=MS.gene003773.t1.exon1;Parent=MS.gene003773.t1
chr4.1 CDS 32933541 32933679 32933541 ID=cds.MS.gene003773.t1;Parent=MS.gene003773.t1
chr4.1 exon 32933171 32933261 32933171 ID=MS.gene003773.t1.exon2;Parent=MS.gene003773.t1
chr4.1 CDS 32933171 32933261 32933171 ID=cds.MS.gene003773.t1;Parent=MS.gene003773.t1
chr4.1 exon 32931801 32932096 32931801 ID=MS.gene003773.t1.exon3;Parent=MS.gene003773.t1
chr4.1 CDS 32931801 32932096 32931801 ID=cds.MS.gene003773.t1;Parent=MS.gene003773.t1
chr4.1 exon 32930877 32930986 32930877 ID=MS.gene003773.t1.exon4;Parent=MS.gene003773.t1
chr4.1 CDS 32930877 32930986 32930877 ID=cds.MS.gene003773.t1;Parent=MS.gene003773.t1
Gene Sequence

>MS.gene003773

ATGACCTTCATTCTTAGCTTATCTCTTTATTCCGTACAGGGTTTTCAAGGCCGAAGTACAGCTAGAGCTGATAGTCCGGTTTCCTTCAGCGATGATGAAGATGCCATGCCAACATCAAGCTCAAAACCAAGCACCAGAGGTCGGAAAGGATCTGTTGCATCTCAAACTACAACCAGAGGAAGAGGAAGGGGAAATAAACCTTAAATTAGGATTACAAATTCATCATTGACAGAAACATCTTGGCCAAATTTTTCTGGGTTACTTTGCCTATCAGGTTCATTGTTTACACATGTTTGAGTCGATTCAGCCACAATGGAAAAGTTTCTTGAAGAAGCCACCAAACTGAAACCAGAAAAACCTCCAACCCTGTTGTCATTATTTCCTTCGCTAACAATAGAAATGCTATTTTCCTCTGCAGGAAACATAGATGACTCCAACTTGCTAGATAAAATCCCTGAATCCATGTGGTCACCACCAGTGTTGGTTGTGGAGAAATTTCTTTCCCTATGGTACTTTAATTTTATTAAGCTTTTTCCTGCTCAATTAGGTTTGCTTCTTATGTGTGTTTAGCTTCTATGTCCATATTGAACTTTACAATTACTATCTAGGTACAACACGAACCTTGACTCTTATAGGGTAATTTGATGAAATATTTATGGCATGGGGCCAAAAACATATATACCATGTTTGTTAAATTTGAATGTTTAGACTAGACATAGTAGTCCTGATTGCAAAGAGATATGGGAGATGAAAAACTTTGATGTGGTCATATACAATTTGTTATTAGGTAGACATTAGAAAAAAATGGAAAAGTGATGAAATTGTAAAAATATTAGGAAAATTCATTGGAAAGAAATGTGTTTTTGCCTGGATGCCTTGATCAAACTCAATATATAGAAAATCCGACAAATATGTTGGAAACAGTTTGGGGATAACTGCAGGGTATGAATAGTTTAATCTCTATCTGAGTTAATTTATTTTGACCATTTGGTAATGGATGGAAGGAGAATGGGGTTATATGTTGTGTTTAAGGGCTTATTTGGATTGGTTTATTTGAGTTTATCTACTTGTGTATGCACTTGTGATACTGTTTGTAAGAGCTTATGAAAACAATTTATGACATGTGCATAACCTGTTTCTAACTCATTTCCCTAAGCTCCTCAAGTATAGCTTATGAATATAACTCATAGCTTATTTCAATAAGCTCTCTAGGACATTGTTTAAATTCAGAATCAGCACAATACATTGAATGCTATTCGTATGAATTGTCTGTTACCATGCAAAAGAATAGTCTGTTTAAGTTATTGAACTTATTTTCTTGGTGGATCCTATTCAATGCACATTGTATGACAGATTTGATCAGAGATCATGCACATGAAAAAAAAGCTGTCTTTCTTTTCCCTCCGGAGTTTCACATTGCGAAAGAGTTAAATAATGGAACTGAGATAATAGTAATGACGAAACTTGCGAAAACCGTGAGTTAAGTCAGAGATGCTTCGTAGAGGACATGTATTGGAATTATCAGTGTTAGATTGAGCAAATATTATTTTCTTTTAATTATAGGTGCTTGTGTTTTTAATTAGGTTGGATATGTCAAATCTGAAAGGAACCTTCTTGCAGAAGTTGATAGAAGTTGATAGCAATTGCATTGTAAAAATTTACTGTTCCTTTCAAGATGACGAGTATTTATATCTCATAATGGAGTATCTACCCAGTGGAGATATGATGACGTTGCTGATGCGGAAAGAAGTACTGACTGAAGATGAAGCTAAGTTGTATATCGGGGAGACTGTGCTATCCATAGAGTCTATTCGTAAACATAATTTATTACTATCTATTCATCCCAAGATTCTTAAGTTGTGTTCATTGGGTTCTTCAGGTTTGTTCATAAATTTTTGTATTTTCATTAGTTAGTCCTCTGATACAGTTTCAAAGTTATAACTAACCATTTTTTGAGTTGTCTTTCCCGTTGATCAAGGAGTGGAATATGAACATTGTTTGATAAATTTTAGTCTTTCTTTCCTTTTCAATGGTATAAGTATAGCTAAGCCATTTTTTAAAGGCAACCTGTGCTCTAAAGCGTTGTTTTTCCCATTGTGCATGCGTTGTAGTCTACAATAGACAAGACGGTCTTACCTTGTCTGTAATGTGAAAGATACTGATTTGACAACCCGAATCTGTAATCTCCTAATTACATGGCAGCAACTCCATCTGTTACATTGTTTGTGTGTTCGGACTCTTTTAAGTTTACATCAGATTGATAGTCATCTGGGTACAAGACTGCTGTTGTTTTTGCATCGACACTTTGGTTTTGTAGATTGCTTGGATTCTGTAGACGCATGTCTCCTTCTACTAACATACTCTTTCTTGCACCAGGTGTTAGATACCCGTGGACATTTGTTGGTTGCCTTGGGGTATGGTTTATGACCGTCGATGGTTCTTATCTCAGAAATTGTCCAGACATTCATCTTAGCAGATTTCTGTTACTATTATGTCAAGAGGTTAACATAGTTTGTACAACCCTGCATTTAATCAGGTCTACTTTACTATTTTCTAAACAAATGAATTGCTATATAAATTATTGTTTAAGAGTTTTTAATAATTAAATTTCTATTCCACAGTTAATGACACATTAAGATTTTTGACTCTAATTCTACATCAATACTCATAATTTCGCCATATCTTTCTAAAAGAAAAAAATAATTACATTTTTCAGGTGGAGTACATCTTGGTTATTGGACATAGTCGCTGCTGCGGAATATCAAGGCTTATGAGTCATCCAAAGGATGGTTCTGCTCCATAG
Protein sequence

>MS.gene003773.t1

MTFILSLSLYSVQGFQGRSTARADSPVSFSDDEDAMPTSSSKPSTRGNIDDSNLLDKIPESMWSPPVLVVEKFLSLWLDMSNLKGTFLQKLIEVDSNCIVKIYCSFQDDEYLYLIMEYLPSGDMMTLLMRKEVLTEDEAKLYIGETVLSIESIRKHNLLLSIHPKILKLCSLGSSEKNNYIFQVEYILVIGHSRCCGISRLMSHPKDGSAP