AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00386

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00386.t1 MTR_2g437940 53.982 226 69 3 6 198 29 252 7.03e-71 221
MS.gene00386.t1 MTR_2g437940 84.211 38 6 0 197 234 337 374 1.75e-13 69.3
MS.gene00386.t1 MTR_2g437180 45.669 254 75 4 6 198 29 280 3.23e-61 197
MS.gene00386.t1 MTR_2g437150 43.651 252 73 4 21 203 42 293 2.71e-60 195
MS.gene00386.t1 MTR_2g437120 48.729 236 59 4 27 201 50 284 3.22e-60 195
MS.gene00386.t1 MTR_2g437170 43.651 252 73 4 21 203 42 293 4.81e-60 194
MS.gene00386.t1 MTR_2g437170 51.389 72 31 2 165 232 339 410 3.86e-11 62.4
MS.gene00386.t1 MTR_2g076320 46.781 233 63 2 27 198 49 281 1.70e-59 192
MS.gene00386.t1 MTR_2g076320 41.221 131 58 5 123 234 273 403 2.06e-16 77.8
MS.gene00386.t1 MTR_2g437960 46.983 232 62 3 28 198 53 284 1.62e-57 187
MS.gene00386.t1 MTR_2g437960 36.296 135 70 5 110 232 274 404 1.03e-11 63.9
MS.gene00386.t1 MTR_2g437960 46.983 232 62 3 28 198 53 284 1.87e-57 188
MS.gene00386.t1 MTR_2g437960 36.567 134 69 5 110 231 274 403 2.64e-11 62.8
MS.gene00386.t1 MTR_2g437260 42.966 263 80 8 1 195 1 261 1.18e-54 180
MS.gene00386.t1 MTR_2g437990 45.690 232 66 4 27 198 49 280 4.99e-54 179
MS.gene00386.t1 MTR_2g437990 40.000 140 56 5 110 231 270 399 1.71e-16 78.2
MS.gene00386.t1 MTR_2g437100 42.017 238 75 3 27 201 59 296 3.27e-53 177
MS.gene00386.t1 MTR_2g437160 48.370 184 58 2 21 198 50 202 7.47e-51 168
MS.gene00386.t1 MTR_2g437160 40.574 244 79 4 21 198 50 293 2.23e-50 169
MS.gene00386.t1 MTR_2g437020 41.079 241 80 3 20 198 29 269 7.25e-50 167
MS.gene00386.t1 MTR_2g437200 69.600 125 32 2 1 121 1 123 7.61e-50 167
MS.gene00386.t1 MTR_2g437200 39.810 211 93 5 35 213 101 309 3.48e-34 126
MS.gene00386.t1 MTR_2g438010 42.060 233 74 3 27 198 69 301 4.69e-49 166
MS.gene00386.t1 MTR_2g438010 58.571 70 27 1 165 232 352 421 1.74e-16 78.2
MS.gene00386.t1 MTR_2g437040 43.172 227 71 3 30 198 42 268 5.86e-49 165
MS.gene00386.t1 MTR_2g437240 61.972 142 48 3 1 138 1 140 1.04e-48 165
MS.gene00386.t1 MTR_2g437240 45.882 170 66 3 58 201 130 299 6.38e-39 139
MS.gene00386.t1 MTR_2g437240 47.727 88 42 2 147 232 331 416 6.06e-13 67.8
MS.gene00386.t1 MTR_2g437030 41.176 255 88 5 19 213 115 367 1.75e-47 163
MS.gene00386.t1 MTR_2g437130 41.667 240 80 4 22 203 37 274 2.04e-45 155
MS.gene00386.t1 MTR_2g437060 42.174 230 75 4 27 198 54 283 1.57e-44 154
MS.gene00386.t1 MTR_4g119550 37.607 234 85 4 24 198 47 278 4.11e-44 152
MS.gene00386.t1 MTR_2g436400 38.372 258 88 5 10 198 20 275 4.75e-44 152
MS.gene00386.t1 MTR_2g438020 78.947 95 18 1 27 121 70 162 5.02e-43 152
MS.gene00386.t1 MTR_2g438020 46.795 156 58 2 68 198 180 335 3.12e-35 130
MS.gene00386.t1 MTR_2g436440 38.000 250 95 4 9 198 25 274 1.13e-42 149
MS.gene00386.t1 MTR_2g436460 39.474 228 77 4 30 198 49 274 5.27e-39 139
MS.gene00386.t1 MTR_4g120380 37.295 244 91 4 28 213 44 283 2.16e-37 134
MS.gene00386.t1 MTR_4g120380 37.295 244 91 4 28 213 44 283 1.05e-36 133
MS.gene00386.t1 MTR_4g119580 35.950 242 93 5 30 213 46 283 3.48e-36 131
MS.gene00386.t1 MTR_2g437080 35.537 242 92 5 19 198 29 268 1.54e-35 130
MS.gene00386.t1 MTR_2g019840 33.047 233 91 5 27 198 42 270 1.25e-28 111
MS.gene00386.t1 MTR_2g436370 47.500 120 54 2 10 120 19 138 2.22e-28 107
MS.gene00386.t1 MTR_4g049400 45.133 113 56 1 21 127 37 149 2.35e-27 105
MS.gene00386.t1 MTR_2g019810 31.602 231 97 4 27 198 30 258 2.01e-24 99.8
MS.gene00386.t1 MTR_4g119570 31.847 157 83 3 76 210 18 172 7.11e-19 82.4
MS.gene00386.t1 MTR_3g085240 52.174 92 33 3 1 83 21 110 2.42e-18 81.6
MS.gene00386.t1 MTR_2g436380 57.143 56 24 0 143 198 1 56 1.51e-15 74.3
MS.gene00386.t1 MTR_2g437090 47.541 61 32 0 143 203 1 61 1.50e-14 69.7
MS.gene00386.t1 MTR_3g450500 22.727 264 133 8 30 227 40 298 1.14e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00386.t1 AT1G61990 27.600 250 121 4 7 198 24 271 4.53e-18 82.8
MS.gene00386.t1 AT1G61990 27.600 250 121 4 7 198 24 271 4.53e-18 82.8
MS.gene00386.t1 AT1G62085 39.167 120 69 2 30 149 56 171 3.45e-17 80.1
MS.gene00386.t1 AT1G62085 39.167 120 69 2 30 149 56 171 3.45e-17 80.1
MS.gene00386.t1 AT1G61980 37.615 109 66 1 30 138 53 159 1.55e-15 75.5
MS.gene00386.t1 AT1G61980 37.615 109 66 1 30 138 53 159 1.55e-15 75.5
MS.gene00386.t1 AT1G61980 37.615 109 66 1 30 138 53 159 1.55e-15 75.5
MS.gene00386.t1 AT1G62120 40.594 101 58 1 28 128 55 153 1.74e-15 75.1
MS.gene00386.t1 AT1G61970 40.404 99 57 1 30 128 53 149 1.03e-14 73.2
MS.gene00386.t1 AT1G61970 40.404 99 57 1 30 128 53 149 1.03e-14 73.2
MS.gene00386.t1 AT1G61970 40.404 99 57 1 30 128 53 149 1.03e-14 73.2
MS.gene00386.t1 AT1G61970 40.404 99 57 1 30 128 53 149 1.03e-14 73.2
MS.gene00386.t1 AT1G61970 40.404 99 57 1 30 128 53 149 1.03e-14 73.2
MS.gene00386.t1 AT1G61970 40.404 99 57 1 30 128 53 149 1.03e-14 73.2
MS.gene00386.t1 AT1G61970 40.404 99 57 1 30 128 53 149 1.03e-14 73.2
MS.gene00386.t1 AT1G21150 40.310 129 68 4 30 153 57 181 1.04e-14 72.8
MS.gene00386.t1 AT3G46950 25.514 243 121 5 30 217 53 290 1.16e-14 72.8
MS.gene00386.t1 AT1G21150 40.310 129 68 4 30 153 121 245 1.77e-14 72.4
MS.gene00386.t1 AT1G21150 39.098 133 72 4 26 153 17 145 3.34e-14 71.2
MS.gene00386.t1 AT1G61960 33.333 120 78 1 9 128 32 149 7.42e-14 70.5
MS.gene00386.t1 AT1G62110 36.364 99 61 1 30 128 53 149 1.41e-11 63.9
MS.gene00386.t1 AT1G62150 32.500 120 77 2 30 149 59 174 1.61e-11 63.5
MS.gene00386.t1 AT5G23930 26.906 223 107 3 30 198 56 276 5.52e-11 62.0
MS.gene00386.t1 AT5G07900 32.110 109 61 2 30 138 70 165 5.60e-11 62.0

Find 40 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCCTTCATTGGCCACTTT+TGG 0.161610 2.2:-51172399 MS.gene00386:CDS
CAAACAAGGCCATCTATATT+TGG 0.171090 2.2:-51172471 MS.gene00386:CDS
CTGAGCGTTGTTGAAGTATT+TGG 0.204791 2.2:+51173113 None:intergenic
CTGGAGGAAGTTAAGTGTTT+AGG 0.230775 2.2:-51172426 MS.gene00386:CDS
TTTCTCTGTCGCAATGCTTT+TGG 0.260075 2.2:-51173140 MS.gene00386:CDS
TGGAGGAAGTTAAGTGTTTA+GGG 0.272754 2.2:-51172425 MS.gene00386:CDS
AAGAATTTGACAACTGAATC+TGG 0.293509 2.2:+51172966 None:intergenic
AAGCTGTTATTCGAGTATTT+AGG 0.318007 2.2:-51172290 MS.gene00386:intron
TGAATCTGACATTGTTCATA+TGG 0.329984 2.2:-51172817 MS.gene00386:CDS
GACATTGTTCATATGGTTAG+AGG 0.340870 2.2:-51172810 MS.gene00386:intron
TCCTACCTCGTCGACAAATT+CGG 0.354152 2.2:-51173056 MS.gene00386:CDS
GTCAGATTCAGAAGCACCTT+TGG 0.364937 2.2:+51172830 None:intergenic
AGATCAAAATCTTATACATT+TGG 0.374741 2.2:+51171921 None:intergenic
GGTTGACGACTTTAAGAAAT+GGG 0.386387 2.2:-51172325 MS.gene00386:CDS
AACTGATTTGATTAAATCAC+TGG 0.402491 2.2:-51172445 MS.gene00386:CDS
TTTAGGGTTTGATCCTTCAT+TGG 0.429686 2.2:-51172409 MS.gene00386:CDS
TCAGTTGAACCAAATATAGA+TGG 0.441181 2.2:+51172462 None:intergenic
AGGTTGACGACTTTAAGAAA+TGG 0.446927 2.2:-51172326 MS.gene00386:CDS
TCAAAATCTTATACATTTGG+TGG 0.453156 2.2:+51171924 None:intergenic
CAAATACAACGGAGAACAAT+GGG 0.473276 2.2:-51171954 MS.gene00386:CDS
ACCCCAAAAGTGGCCAATGA+AGG 0.496707 2.2:+51172396 None:intergenic
AATCTTATACATTTGGTGGA+TGG 0.528179 2.2:+51171928 None:intergenic
AAGGGTGTGAAGTGATGGTA+AGG 0.538274 2.2:+51173087 None:intergenic
AGCGAAAGGGTGTGAAGTGA+TGG 0.551250 2.2:+51173082 None:intergenic
TACCAAAGCAACCCCAAAAG+TGG 0.559263 2.2:+51172386 None:intergenic
TTTCAGCTTTAAGGAGGAGT+CGG 0.563069 2.2:-51172016 MS.gene00386:intron
GCAAATACAACGGAGAACAA+TGG 0.572984 2.2:-51171955 MS.gene00386:CDS
TCGCTAATTTGCTTCAAACA+AGG 0.580422 2.2:-51172485 MS.gene00386:CDS
TCCGAATTTGTCGACGAGGT+AGG 0.584234 2.2:+51173055 None:intergenic
GCTTCAGAATCAGAACACCT+TGG 0.592387 2.2:-51173008 MS.gene00386:CDS
CGAGGTAGGACACAGCGAAA+GGG 0.593390 2.2:+51173069 None:intergenic
ACGACTTTAAGAAATGGGGT+TGG 0.600597 2.2:-51172320 MS.gene00386:CDS
ACGAGGTAGGACACAGCGAA+AGG 0.604629 2.2:+51173068 None:intergenic
GTTGACGACTTTAAGAAATG+GGG 0.629997 2.2:-51172324 MS.gene00386:CDS
TTCACTGACTCAGACATGAA+AGG 0.637892 2.2:-51172930 MS.gene00386:CDS
AATGAAACTTGCAAATACAA+CGG 0.655557 2.2:-51171965 MS.gene00386:CDS
TGATTTGATTAAATCACTGG+AGG 0.658835 2.2:-51172442 MS.gene00386:CDS
GTATTGCAGGAAAGAAGCCA+TGG 0.665041 2.2:+51172891 None:intergenic
GCATCATCAAAAGAGAACCA+TGG 0.686492 2.2:-51172908 MS.gene00386:CDS
AGAATCCGAATTTGTCGACG+AGG 0.712975 2.2:+51173051 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAACAATTCAAAAAGA+GGG + chr2.2:51172439-51172458 None:intergenic 15.0%
!! TTTCTTATAGTTTACATAAA+AGG - chr2.2:51172921-51172940 MS.gene00386:CDS 15.0%
!! AAACTATAAGAAAACATAAG+TGG + chr2.2:51172914-51172933 None:intergenic 20.0%
!! AATGTTTGAGTAAAAAACTT+TGG - chr2.2:51172720-51172739 MS.gene00386:intron 20.0%
!! AGAGTAAAAATTGAAACTTT+GGG + chr2.2:51172226-51172245 None:intergenic 20.0%
!! ATGTTTGAGTAAAAAACTTT+GGG - chr2.2:51172721-51172740 MS.gene00386:intron 20.0%
!! CTATTTAACAATTCAAAAAG+AGG + chr2.2:51172440-51172459 None:intergenic 20.0%
!!! ATCAATTTGATGATTGATTT+TGG - chr2.2:51172551-51172570 MS.gene00386:intron 20.0%
!!! CTCTTTTTGAATTGTTAAAT+AGG - chr2.2:51172438-51172457 MS.gene00386:CDS 20.0%
!!! TTTGAATATTTTCTATCCAA+AGG - chr2.2:51172335-51172354 MS.gene00386:CDS 20.0%
!!! TTTTTTACTCAAACATTTCT+TGG + chr2.2:51172717-51172736 None:intergenic 20.0%
! AACTTCTCAGAATAAGTAAA+TGG + chr2.2:51173022-51173041 None:intergenic 25.0%
! AAGCTAATGTGTAAAATTGT+AGG + chr2.2:51172401-51172420 None:intergenic 25.0%
! AATGAAACTTGCAAATACAA+CGG - chr2.2:51173111-51173130 MS.gene00386:CDS 25.0%
! AATTGATATTAGTCACCATA+AGG + chr2.2:51172538-51172557 None:intergenic 25.0%
! CAAAAGAAATTGCAAAACTA+AGG + chr2.2:51172960-51172979 None:intergenic 25.0%
! GAGAGTAAAAATTGAAACTT+TGG + chr2.2:51172227-51172246 None:intergenic 25.0%
! GTCAAATTCTTCAAAAATCA+CGG - chr2.2:51172122-51172141 MS.gene00386:intron 25.0%
! TCAAAATCTTATACATTTGG+TGG + chr2.2:51173155-51173174 None:intergenic 25.0%
!! CAACAGATTAATCTTTTCAA+TGG + chr2.2:51172837-51172856 None:intergenic 25.0%
!! TACAATTTTACACATTAGCT+TGG - chr2.2:51172400-51172419 MS.gene00386:CDS 25.0%
!! TTGCAATTTCTTTTGATGAA+AGG - chr2.2:51172965-51172984 MS.gene00386:CDS 25.0%
!! TTTCAATTTTTACTCTCCAA+AGG - chr2.2:51172230-51172249 MS.gene00386:intron 25.0%
!!! AACTGATTTGATTAAATCAC+TGG - chr2.2:51172631-51172650 MS.gene00386:intron 25.0%
!!! AGTTTTTGGGTTAATCAAAT+GGG - chr2.2:51172860-51172879 MS.gene00386:CDS 25.0%
!!! TAATCTGTTGATGAGTTTTT+GGG - chr2.2:51172847-51172866 MS.gene00386:CDS 25.0%
!!! TTAATCTGTTGATGAGTTTT+TGG - chr2.2:51172846-51172865 MS.gene00386:CDS 25.0%
AAGAATTTGACAACTGAATC+TGG + chr2.2:51172113-51172132 None:intergenic 30.0%
AAGCTGTTATTCGAGTATTT+AGG - chr2.2:51172786-51172805 MS.gene00386:intron 30.0%
AAGCTTAATTCCAAGAACTT+AGG + chr2.2:51172296-51172315 None:intergenic 30.0%
ACATAAAAGGATCATTCCAA+GGG - chr2.2:51172934-51172953 MS.gene00386:CDS 30.0%
AGCTTAATTCCAAGAACTTA+GGG + chr2.2:51172295-51172314 None:intergenic 30.0%
ATGGAAACCAACAAAATACT+AGG + chr2.2:51172818-51172837 None:intergenic 30.0%
ATGTCGAATTAAGCAAACAA+TGG + chr2.2:51172486-51172505 None:intergenic 30.0%
TACATAAAAGGATCATTCCA+AGG - chr2.2:51172933-51172952 MS.gene00386:CDS 30.0%
TCAGTTGAACCAAATATAGA+TGG + chr2.2:51172617-51172636 None:intergenic 30.0%
TGAATCTGACATTGTTCATA+TGG - chr2.2:51172259-51172278 MS.gene00386:intron 30.0%
TGATTTGATTAAATCACTGG+AGG - chr2.2:51172634-51172653 MS.gene00386:intron 30.0%
! AATCTTATACATTTGGTGGA+TGG + chr2.2:51173151-51173170 None:intergenic 30.0%
!! CAAGTTTGTTTTCAGCTTTA+AGG - chr2.2:51173051-51173070 MS.gene00386:CDS 30.0%
!! TAAAAAACTTTGGGATGAGA+AGG - chr2.2:51172730-51172749 MS.gene00386:intron 30.0%
!!! GAGTTTTTGGGTTAATCAAA+TGG - chr2.2:51172859-51172878 MS.gene00386:CDS 30.0%
CAAACAAGGCCATCTATATT+TGG - chr2.2:51172605-51172624 MS.gene00386:intron 35.0%
CAAATACAACGGAGAACAAT+GGG - chr2.2:51173122-51173141 MS.gene00386:CDS 35.0%
GACATTGTTCATATGGTTAG+AGG - chr2.2:51172266-51172285 MS.gene00386:intron 35.0%
TAGGTTCTTCAAATCCAGTA+AGG - chr2.2:51172457-51172476 MS.gene00386:CDS 35.0%
TGGAGGAAGTTAAGTGTTTA+GGG - chr2.2:51172651-51172670 MS.gene00386:intron 35.0%
! AGGTTGACGACTTTAAGAAA+TGG - chr2.2:51172750-51172769 MS.gene00386:intron 35.0%
! GGTTGACGACTTTAAGAAAT+GGG - chr2.2:51172751-51172770 MS.gene00386:intron 35.0%
! GTTGACGACTTTAAGAAATG+GGG - chr2.2:51172752-51172771 MS.gene00386:intron 35.0%
! TTGGGTTAATCAAATGGGTT+GGG - chr2.2:51172865-51172884 MS.gene00386:CDS 35.0%
! TTTAGGGTTTGATCCTTCAT+TGG - chr2.2:51172667-51172686 MS.gene00386:intron 35.0%
! TTTGGGTTAATCAAATGGGT+TGG - chr2.2:51172864-51172883 MS.gene00386:CDS 35.0%
!! AATTGTTTTGTGGGTATTGC+AGG + chr2.2:51172201-51172220 None:intergenic 35.0%
!! ACTTTGGGAGAATTGTTTTG+TGG + chr2.2:51172211-51172230 None:intergenic 35.0%
!! CTTTGGGAGAATTGTTTTGT+GGG + chr2.2:51172210-51172229 None:intergenic 35.0%
!! TCGCTAATTTGCTTCAAACA+AGG - chr2.2:51172591-51172610 MS.gene00386:intron 35.0%
!! TTTTAATTGCCATCGCCTTA+TGG - chr2.2:51172520-51172539 MS.gene00386:intron 35.0%
!!! AACTGAATCTGGTTTTAGTG+AGG + chr2.2:51172102-51172121 None:intergenic 35.0%
!!! GTTTGTTTTCAGCTTTAAGG+AGG - chr2.2:51173054-51173073 MS.gene00386:CDS 35.0%
!!! TTAGTGAGGTTTTGAAACCA+AGG + chr2.2:51172088-51172107 None:intergenic 35.0%
AATTGCAAAACTAAGGCCCT+TGG + chr2.2:51172953-51172972 None:intergenic 40.0%
CTGGAGGAAGTTAAGTGTTT+AGG - chr2.2:51172650-51172669 MS.gene00386:intron 40.0%
GCAAACAATGGTATCCTTAC+TGG + chr2.2:51172474-51172493 None:intergenic 40.0%
GCAAATACAACGGAGAACAA+TGG - chr2.2:51173121-51173140 MS.gene00386:CDS 40.0%
GCATCATCAAAAGAGAACCA+TGG - chr2.2:51172168-51172187 MS.gene00386:intron 40.0%
TAGAGGTAACCCTAAGTTCT+TGG - chr2.2:51172283-51172302 MS.gene00386:intron 40.0%
TATTAGTCACCATAAGGCGA+TGG + chr2.2:51172532-51172551 None:intergenic 40.0%
TGGACATTTAGTAAGTGCCA+AGG + chr2.2:51172894-51172913 None:intergenic 40.0%
! ACGACTTTAAGAAATGGGGT+TGG - chr2.2:51172756-51172775 MS.gene00386:intron 40.0%
! CTGAGCGTTGTTGAAGTATT+TGG + chr2.2:51171966-51171985 None:intergenic 40.0%
! GTGTCGTCCTAGTATTTTGT+TGG - chr2.2:51172808-51172827 MS.gene00386:intron 40.0%
! TTTCTCTGTCGCAATGCTTT+TGG - chr2.2:51171936-51171955 MS.gene00386:CDS 40.0%
!! ATCAGATGAAGAAGCACCTT+TGG + chr2.2:51172354-51172373 None:intergenic 40.0%
!! TTCACTGACTCAGACATGAA+AGG - chr2.2:51172146-51172165 MS.gene00386:intron 40.0%
!! TTTCAGCTTTAAGGAGGAGT+CGG - chr2.2:51173060-51173079 MS.gene00386:CDS 40.0%
!!! ATCCTTCATTGGCCACTTTT+GGG - chr2.2:51172678-51172697 MS.gene00386:intron 40.0%
AGAATCCGAATTTGTCGACG+AGG + chr2.2:51172028-51172047 None:intergenic 45.0%
GCTTCAGAATCAGAACACCT+TGG - chr2.2:51172068-51172087 MS.gene00386:intron 45.0%
GTATTGCAGGAAAGAAGCCA+TGG + chr2.2:51172188-51172207 None:intergenic 45.0%
TACCAAAGCAACCCCAAAAG+TGG + chr2.2:51172693-51172712 None:intergenic 45.0%
TCCTACCTCGTCGACAAATT+CGG - chr2.2:51172020-51172039 MS.gene00386:intron 45.0%
! AAGGGTGTGAAGTGATGGTA+AGG + chr2.2:51171992-51172011 None:intergenic 45.0%
!! GATCCTTCATTGGCCACTTT+TGG - chr2.2:51172677-51172696 MS.gene00386:intron 45.0%
!! GTCAGATTCAGAAGCACCTT+TGG + chr2.2:51172249-51172268 None:intergenic 45.0%
!! TCAAATGGGTTGGGATTCCT+TGG - chr2.2:51172874-51172893 MS.gene00386:CDS 45.0%
!!! TCCTTCATTGGCCACTTTTG+GGG - chr2.2:51172679-51172698 MS.gene00386:intron 45.0%
ACCCCAAAAGTGGCCAATGA+AGG + chr2.2:51172683-51172702 None:intergenic 50.0%
TCCGAATTTGTCGACGAGGT+AGG + chr2.2:51172024-51172043 None:intergenic 50.0%
! AGCGAAAGGGTGTGAAGTGA+TGG + chr2.2:51171997-51172016 None:intergenic 50.0%
ACGAGGTAGGACACAGCGAA+AGG + chr2.2:51172011-51172030 None:intergenic 55.0%
CGAGGTAGGACACAGCGAAA+GGG + chr2.2:51172010-51172029 None:intergenic 55.0%
!!! GGCCACTTTTGGGGTTGCTT+TGG - chr2.2:51172688-51172707 MS.gene00386:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 51171924 51173174 51171924 ID=MS.gene00386
chr2.2 mRNA 51171924 51173174 51171924 ID=MS.gene00386.t1;Parent=MS.gene00386
chr2.2 exon 51172811 51173174 51172811 ID=MS.gene00386.t1.exon1;Parent=MS.gene00386.t1
chr2.2 CDS 51172811 51173174 51172811 ID=cds.MS.gene00386.t1;Parent=MS.gene00386.t1
chr2.2 exon 51172291 51172522 51172291 ID=MS.gene00386.t1.exon2;Parent=MS.gene00386.t1
chr2.2 CDS 51172291 51172522 51172291 ID=cds.MS.gene00386.t1;Parent=MS.gene00386.t1
chr2.2 exon 51171924 51172032 51171924 ID=MS.gene00386.t1.exon3;Parent=MS.gene00386.t1
chr2.2 CDS 51171924 51172032 51171924 ID=cds.MS.gene00386.t1;Parent=MS.gene00386.t1
Gene Sequence

>MS.gene00386

ATGTTGAAAGCATTTCTCTGTCGCAATGCTTTTGGCATACCAAATACTTCAACAACGCTCAGATTCCTTACCATCACTTCACACCCTTTCGCTGTGTCCTACCTCGTCGACAAATTCGGATTCTCATATGAATTTGCACTCAAAGCTTCAGAATCAGAACACCTTGGTTTCAAAACCTCACTAAAACCAGATTCAGTTGTCAAATTCTTCAAAAATCACGGTTTCACTGACTCAGACATGAAAGGCATCATCAAAAGAGAACCATGGCTTCTTTCCTGCAATACCCACAAAACAATTCTCCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTGAATCTGACATTGTTCATATGGTTAGAGGTAACCCTAAGTTCTTGGAATTAAGCTTGAAGAATCATCAAATTAAGTTTGAATATTTTCTATCCAAAGGTGCTTCTTCATCTGATATTGTTTCATTGTTAACTAAAAATCCTACAATTTTACACATTAGCTTGGAGAAACAAATAATCCCTCTTTTTGAATTGTTAAATAGGTTCTTCAAATCCAGTAAGGATACCATTGTTTGCTTAATTCGACATTCAACTACATTTGCTTTTAATTGCCATCGCCTTATGGTGACTAATATCAATTTGATGATTGATTTTGGAGTTTCTGATTCTATCATCGCTAATTTGCTTCAAACAAGGCCATCTATATTTGGTTCAACTGATTTGATTAAATCACTGGAGGAAGTTAAGTGTTTAGGGTTTGATCCTTCATTGGCCACTTTTGGGGTTGCTTTGGTAGCCAAGAAATGTTTGAGTAAAAAACTTTGGGATGAGAAGGTTGACGACTTTAAGAAATGGGGTTGGTCTGATGAAGCTGTTATTCGAGTATTTAGGTGTCGTCCTAGTATTTTGTTGGTTTCCATTGAAAAGATTAATCTGTTGATGAGTTTTTGGGTTAATCAAATGGGTTGGGATTCCTTGGCACTTACTAAATGTCCACTTATGTTTTCTTATAGTTTACATAAAAGGATCATTCCAAGGGCCTTAGTTTTGCAATTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCAAGCTTAGTTATACCATTTACTTATTCTGAGAAGTTGTTCTTGAACAAGTTTGTTTTCAGCTTTAAGGAGGAGTCGGATTATCTGTTGAAGCTATATGACGAAAAAATGAAACTTGCAAATACAACGGAGAACAATGGGATGCCATCCACCAAATGTATAAGATTTTGA
Protein sequence

>MS.gene00386.t1

MLKAFLCRNAFGIPNTSTTLRFLTITSHPFAVSYLVDKFGFSYEFALKASESEHLGFKTSLKPDSVVKFFKNHGFTDSDMKGIIKREPWLLSCNTHKTILPKFQFLLSKGASESDIVHMVRVSDSIIANLLQTRPSIFGSTDLIKSLEEVKCLGFDPSLATFGVALVAKKCLSKKLWDEKVDDFKKWGWSDEAVIRVFSFKEESDYLLKLYDEKMKLANTTENNGMPSTKCIRF