AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003882

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003882.t1 MTR_4g067250 91.429 490 41 1 3 491 91 580 0.0 854
MS.gene003882.t1 MTR_8g091830 51.005 398 166 7 121 491 363 758 5.57e-97 309
MS.gene003882.t1 MTR_8g091830 50.761 394 169 6 121 491 365 756 1.36e-93 302
MS.gene003882.t1 MTR_8g091850 40.532 301 125 7 111 409 46 294 7.08e-54 183
MS.gene003882.t1 MTR_8g091840 56.140 57 24 1 436 491 9 65 3.83e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003882.t1 AT4G39160 38.372 258 134 8 178 423 277 521 2.92e-35 139
MS.gene003882.t1 AT4G39160 38.372 258 134 8 178 423 277 521 2.92e-35 139
MS.gene003882.t1 AT4G39160 38.372 258 134 8 178 423 277 521 3.32e-35 139

Find 83 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTCAATCAATTTCTCTT+AGG 0.163126 4.2:-38560472 MS.gene003882:CDS
TATGCAATTCCCATTCTTTC+AGG 0.200971 4.2:-38559796 MS.gene003882:CDS
TCTTGCAATTGCAGAAAATA+AGG 0.274714 4.2:-38560444 MS.gene003882:CDS
TCTATATTGTTACAAATATA+AGG 0.289417 4.2:+38560813 None:intergenic
CACAGTGGACCAGGCTCTGC+TGG 0.334784 4.2:-38560510 MS.gene003882:intron
GTTGAATCATTGTAAAATCT+AGG 0.346657 4.2:+38559559 None:intergenic
ACAAGTCACCTAGGGTAAAA+TGG 0.357410 4.2:-38559772 MS.gene003882:CDS
CTGCTGGATGATTATGAACT+TGG 0.367304 4.2:-38560494 MS.gene003882:CDS
GGCTTCCTGATTTGCATTAG+TGG 0.370648 4.2:+38559248 None:intergenic
CCTGAGACCAACCTCAATAA+TGG 0.378901 4.2:-38561553 MS.gene003882:intron
GTTGGCTTCAAAAGTGCTTC+AGG 0.382886 4.2:-38561286 MS.gene003882:CDS
TACTAGCTTGATCATCCTCT+TGG 0.383958 4.2:+38559843 None:intergenic
TTCTACCACTAATGCAAATC+AGG 0.389275 4.2:-38559253 MS.gene003882:CDS
TTCTCAATCAATTTCTCTTA+GGG 0.392485 4.2:-38560471 MS.gene003882:CDS
CATGCTACCTCGTCTGTAGA+TGG 0.395737 4.2:-38561622 MS.gene003882:CDS
GGCGATTTCTTTCATGATGC+TGG 0.401167 4.2:-38559917 MS.gene003882:CDS
AAAAGTTCTCTCGTTCTTCT+CGG 0.411529 4.2:-38560629 MS.gene003882:CDS
ACATAGGCTCTGAAGGAGTT+TGG 0.412678 4.2:-38559582 MS.gene003882:intron
GCTATTAATAGTCGTTCATC+AGG 0.426472 4.2:-38559453 MS.gene003882:intron
CGTAAAAGATCATCTGTTGC+TGG 0.432583 4.2:-38560945 MS.gene003882:CDS
TTTGACCTTCGGCGCAAACT+TGG 0.442856 4.2:+38561684 None:intergenic
GTGACTTGTCCCTGAAAGAA+TGG 0.455083 4.2:+38559786 None:intergenic
ATGCAATTCCCATTCTTTCA+GGG 0.455410 4.2:-38559795 MS.gene003882:CDS
CCATTATTGAGGTTGGTCTC+AGG 0.458534 4.2:+38561553 None:intergenic
TTCAGATATTTCCTTTCGTA+AGG 0.463522 4.2:+38561651 None:intergenic
GTGATGATGACTTACGGAAA+TGG 0.472624 4.2:-38558591 MS.gene003882:CDS
AGAGTGCATACATTAATTCC+AGG 0.475172 4.2:+38559198 None:intergenic
TTTCATTCCCACAGTGGACC+AGG 0.476047 4.2:-38560519 MS.gene003882:intron
CTGTGTTGCTTACCTTCGAT+TGG 0.477352 4.2:+38560283 None:intergenic
CAGTGCTGATGTTAAAGATC+AGG 0.478050 4.2:-38558656 MS.gene003882:CDS
AAACATAATTACCATTATTG+AGG 0.480467 4.2:+38561542 None:intergenic
AATGGAATGAATGTTGTTCT+CGG 0.482678 4.2:+38561243 None:intergenic
ATAATTACCATTATTGAGGT+TGG 0.490657 4.2:+38561546 None:intergenic
GCATTAGTGGTAGAAATTTG+TGG 0.499145 4.2:+38559261 None:intergenic
TGTTGCTTACCTTCGATTGG+TGG 0.499150 4.2:+38560286 None:intergenic
TCACTATCATCAGATTGCTC+TGG 0.502518 4.2:+38558610 None:intergenic
TGCAGAAAATAAGGAGCGAC+CGG 0.506681 4.2:-38560435 MS.gene003882:CDS
TAGTGCCAAGTTTGCGCCGA+AGG 0.506770 4.2:-38561689 MS.gene003882:CDS
ATTGAGGTTGGTCTCAGGAA+TGG 0.510269 4.2:+38561558 None:intergenic
ATAGCATCAGTTATTCGTAA+AGG 0.518506 4.2:+38559471 None:intergenic
AAAGTCAAACTCATTGATTG+AGG 0.522023 4.2:-38560856 MS.gene003882:CDS
CATAATCATCCAGCAGAGCC+TGG 0.529086 4.2:+38560501 None:intergenic
TGACTTGTCCCTGAAAGAAT+GGG 0.530560 4.2:+38559787 None:intergenic
CTGTCAAGGAATGCAGTGGA+TGG 0.545069 4.2:+38561355 None:intergenic
CTCTGGACTATGATACGCCA+TGG 0.555687 4.2:+38558627 None:intergenic
AAGATCATCTGTTGCTGGTG+TGG 0.557955 4.2:-38560940 MS.gene003882:CDS
AATGGATTTAGTGACGGTGG+GGG 0.564034 4.2:+38561576 None:intergenic
GGAATGGATTTAGTGACGGT+GGG 0.575171 4.2:+38561574 None:intergenic
GTCAAGGAATGCAGTGGATG+GGG 0.578734 4.2:+38561357 None:intergenic
ATGATAGTGATGATGACTTA+CGG 0.580140 4.2:-38558597 MS.gene003882:CDS
TTGCAAGACAGCCTAAATGT+TGG 0.580193 4.2:-38561304 MS.gene003882:CDS
TTTATTAGGATGCTAATGAG+AGG 0.585716 4.2:-38560986 MS.gene003882:intron
ATCAAAACAGTCCTTACGAA+AGG 0.590154 4.2:-38561662 MS.gene003882:CDS
ACTACTGAAGACATGTCACC+TGG 0.592887 4.2:-38559216 MS.gene003882:CDS
AAATGATGACGAGGACGAAG+TGG 0.594931 4.2:-38560778 MS.gene003882:CDS
GTCTTCAGTAGTGTCTTCAG+TGG 0.600402 4.2:+38559227 None:intergenic
CTTTCAGGGACAAGTCACCT+AGG 0.604637 4.2:-38559781 MS.gene003882:CDS
TGCTGATGTTAAAGATCAGG+AGG 0.606209 4.2:-38558653 MS.gene003882:CDS
CTCAGGAATGGATTTAGTGA+CGG 0.612639 4.2:+38561570 None:intergenic
CAAAGTCTTCCACCAATCGA+AGG 0.613148 4.2:-38560295 MS.gene003882:intron
TTTCAGGGACAAGTCACCTA+GGG 0.617228 4.2:-38559780 MS.gene003882:CDS
AGGAATGCAGTGGATGGGGA+AGG 0.617655 4.2:+38561361 None:intergenic
GAATGGATTTAGTGACGGTG+GGG 0.617902 4.2:+38561575 None:intergenic
TGTCAAGGAATGCAGTGGAT+GGG 0.621373 4.2:+38561356 None:intergenic
TTCGGCGCAAACTTGGCACT+AGG 0.622860 4.2:+38561691 None:intergenic
ATGACCGTGGTAAAAGCTCA+AGG 0.624975 4.2:-38560914 MS.gene003882:CDS
ATCATCTGTTGCTGGTGTGG+AGG 0.632127 4.2:-38560937 MS.gene003882:CDS
ATATTGTTACAAATATAAGG+AGG 0.636102 4.2:+38560816 None:intergenic
AGGAATGGATTTAGTGACGG+TGG 0.638656 4.2:+38561573 None:intergenic
TAAAGATCAGGAGGATCCCA+TGG 0.641855 4.2:-38558644 MS.gene003882:CDS
AGAAAATAAGGAGCGACCGG+AGG 0.647366 4.2:-38560432 MS.gene003882:intron
GTTGCTGTCAAGGAATGCAG+TGG 0.651362 4.2:+38561351 None:intergenic
TATTGTTACAAATATAAGGA+GGG 0.653546 4.2:+38560817 None:intergenic
TACTTCCGGAAAAGTACAAG+AGG 0.653833 4.2:-38561218 MS.gene003882:intron
TCTGGACTATGATACGCCAT+GGG 0.656583 4.2:+38558628 None:intergenic
AGAGGTTGCAACAACTGAGG+AGG 0.662600 4.2:-38558680 MS.gene003882:CDS
TTATTCGTAAAGGATGATGT+CGG 0.671321 4.2:+38559481 None:intergenic
GGAAGAGGTTGCAACAACTG+AGG 0.674245 4.2:-38558683 MS.gene003882:CDS
TGCTGGTGCTAATAGTGAAG+AGG 0.679934 4.2:-38559900 MS.gene003882:CDS
ACTTGAAGAAAATGATGACG+AGG 0.685696 4.2:-38560787 MS.gene003882:CDS
GCTCTTTCCATCTACAGACG+AGG 0.706731 4.2:+38561615 None:intergenic
TGATGGTTGTAGAGACCAAG+AGG 0.723191 4.2:-38559858 MS.gene003882:CDS
GCTGGTGTGGAGGATGACCG+TGG 0.747382 4.2:-38560927 MS.gene003882:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAATATAATTGACA+AGG + chr4.2:38560252-38560271 None:intergenic 10.0%
!!! TTTCATTTGTAATTTTTATT+AGG - chr4.2:38559359-38559378 MS.gene003882:intron 10.0%
!! AATATAAATCTATTTACTAC+TGG + chr4.2:38560393-38560412 None:intergenic 15.0%
!! TAAGAAATATTACAATTATG+TGG + chr4.2:38561605-38561624 None:intergenic 15.0%
!! TCTATATTGTTACAAATATA+AGG + chr4.2:38559549-38559568 None:intergenic 15.0%
!!! TGTTCATTTTTTTAAAACAT+AGG - chr4.2:38560761-38560780 MS.gene003882:CDS 15.0%
!!! TTGTTTTGTTTTATATTTTG+AGG - chr4.2:38561188-38561207 MS.gene003882:intron 15.0%
!! AAACATAATTACCATTATTG+AGG + chr4.2:38558820-38558839 None:intergenic 20.0%
!! AAGTTCAAAAAAGAAATATG+TGG - chr4.2:38559635-38559654 MS.gene003882:intron 20.0%
!! ATATTAGGGTTTAAATTACA+TGG + chr4.2:38561389-38561408 None:intergenic 20.0%
!! ATATTGTTACAAATATAAGG+AGG + chr4.2:38559546-38559565 None:intergenic 20.0%
!! GATATTTGTGAGTTAAAATT+TGG - chr4.2:38561294-38561313 MS.gene003882:CDS 20.0%
!! TATTGTTACAAATATAAGGA+GGG + chr4.2:38559545-38559564 None:intergenic 20.0%
!! TTCTAAATCTATAAATGAAG+TGG + chr4.2:38561567-38561586 None:intergenic 20.0%
!!! AGATCATCATATTTTTAAGT+CGG - chr4.2:38561061-38561080 MS.gene003882:intron 20.0%
!!! ATGATTCAACAAATTTTTTC+TGG - chr4.2:38560813-38560832 MS.gene003882:CDS 20.0%
!!! ATTACTAATTTTGTCTTTTC+TGG - chr4.2:38560696-38560715 MS.gene003882:CDS 20.0%
!!! ATTATATTCTTTTTGTAGCT+CGG - chr4.2:38558645-38558664 MS.gene003882:CDS 20.0%
!!! TTTAAAATATTTATCTGCAG+TGG - chr4.2:38560418-38560437 MS.gene003882:intron 20.0%
! AAGAAATATGTGGCTAAAAA+TGG - chr4.2:38559645-38559664 MS.gene003882:intron 25.0%
! ATAATTACCATTATTGAGGT+TGG + chr4.2:38558816-38558835 None:intergenic 25.0%
! ATATATGTCTTTATTGTGCT+AGG + chr4.2:38561541-38561560 None:intergenic 25.0%
! ATATGACGTAAAAACTTTCA+AGG - chr4.2:38561510-38561529 MS.gene003882:intron 25.0%
! ATGATGGTAAATAAGATGTT+TGG + chr4.2:38560370-38560389 None:intergenic 25.0%
! CACATATATCTTAACTTCTT+AGG + chr4.2:38560162-38560181 None:intergenic 25.0%
! GTTGAATCATTGTAAAATCT+AGG + chr4.2:38560803-38560822 None:intergenic 25.0%
! TAAAATGTTATTCCACATCA+AGG + chr4.2:38561012-38561031 None:intergenic 25.0%
! TACAAAAAAAGCAAGGAAAA+TGG - chr4.2:38559675-38559694 MS.gene003882:intron 25.0%
! TGTAAAAAATCAGCTAATGT+AGG + chr4.2:38559238-38559257 None:intergenic 25.0%
! TTCTCAATCAATTTCTCTTA+GGG - chr4.2:38559888-38559907 MS.gene003882:CDS 25.0%
!! AAAAGCATACAAAAAAAGCA+AGG - chr4.2:38559668-38559687 MS.gene003882:intron 25.0%
!! AATTGTTATTAGTAGGCTTA+TGG - chr4.2:38559991-38560010 MS.gene003882:intron 25.0%
!! ATTTTGTCTTTTCTGGATTT+AGG - chr4.2:38560703-38560722 MS.gene003882:CDS 25.0%
!! GAATACCTCATAAAACTTTT+CGG + chr4.2:38560626-38560645 None:intergenic 25.0%
!! TGAGAATCCATTTTATACAA+GGG - chr4.2:38561459-38561478 MS.gene003882:intron 25.0%
!! TGTATAAAATGGATTCTCAA+GGG + chr4.2:38561458-38561477 None:intergenic 25.0%
!! TTGAGAATCCATTTTATACA+AGG - chr4.2:38561458-38561477 MS.gene003882:intron 25.0%
!! TTGTATAAAATGGATTCTCA+AGG + chr4.2:38561459-38561478 None:intergenic 25.0%
!!! ATTCATTCCATTTTTACTTC+CGG - chr4.2:38559127-38559146 MS.gene003882:intron 25.0%
!!! TTTTGAGGTCTATATTCATT+TGG - chr4.2:38561203-38561222 MS.gene003882:intron 25.0%
AAAATATTTATCTGCAGTGG+TGG - chr4.2:38560421-38560440 MS.gene003882:intron 30.0%
AAATCGTGAAAGCTAACAAA+GGG + chr4.2:38560225-38560244 None:intergenic 30.0%
AAATGTGGTCTCTTATTAGA+TGG - chr4.2:38558950-38558969 MS.gene003882:intron 30.0%
AAGAGTCTTGAATATTTGCA+AGG + chr4.2:38561640-38561659 None:intergenic 30.0%
AATGGAATGAATGTTGTTCT+CGG + chr4.2:38559119-38559138 None:intergenic 30.0%
ATAGCATCAGTTATTCGTAA+AGG + chr4.2:38560891-38560910 None:intergenic 30.0%
ATATACGCTGCTTCTATATT+AGG + chr4.2:38561404-38561423 None:intergenic 30.0%
ATCTATTTACTACTGGATGA+TGG + chr4.2:38560386-38560405 None:intergenic 30.0%
ATGAGAGGAAAAAATCTCTA+AGG - chr4.2:38559388-38559407 MS.gene003882:intron 30.0%
CATATAGTACATGATCAGTA+GGG - chr4.2:38560966-38560985 MS.gene003882:CDS 30.0%
GAAGTAAATTTGAGTAACGA+AGG + chr4.2:38561367-38561386 None:intergenic 30.0%
GTCATATCACATATACATGA+TGG + chr4.2:38561497-38561516 None:intergenic 30.0%
GTGTATTATCAAAGCAGTAT+TGG - chr4.2:38560106-38560125 MS.gene003882:intron 30.0%
GTTCTCAATCAATTTCTCTT+AGG - chr4.2:38559887-38559906 MS.gene003882:CDS 30.0%
TAAATCGTGAAAGCTAACAA+AGG + chr4.2:38560226-38560245 None:intergenic 30.0%
TATACGCTGCTTCTATATTA+GGG + chr4.2:38561403-38561422 None:intergenic 30.0%
TCATATAGTACATGATCAGT+AGG - chr4.2:38560965-38560984 MS.gene003882:CDS 30.0%
TCTTGCAATTGCAGAAAATA+AGG - chr4.2:38559915-38559934 MS.gene003882:CDS 30.0%
TGTTGTTCCCTTGTATAAAA+TGG + chr4.2:38561469-38561488 None:intergenic 30.0%
TTATTCGTAAAGGATGATGT+CGG + chr4.2:38560881-38560900 None:intergenic 30.0%
TTCAGATATTTCCTTTCGTA+AGG + chr4.2:38558711-38558730 None:intergenic 30.0%
TTTATTAGGATGCTAATGAG+AGG - chr4.2:38559373-38559392 MS.gene003882:intron 30.0%
! AAAGTCAAACTCATTGATTG+AGG - chr4.2:38559503-38559522 MS.gene003882:CDS 30.0%
! ATGATAGTGATGATGACTTA+CGG - chr4.2:38561762-38561781 MS.gene003882:intron 30.0%
! CCAAAATTAGCACTTTTACT+TGG - chr4.2:38561335-38561354 MS.gene003882:CDS 30.0%
! GCATTGACAACTATTAAATG+TGG - chr4.2:38558935-38558954 MS.gene003882:intron 30.0%
! TACTTTTCCGGAAGTAAAAA+TGG + chr4.2:38559137-38559156 None:intergenic 30.0%
! TCCTAGCAATTGTTATTAGT+AGG - chr4.2:38559984-38560003 MS.gene003882:intron 30.0%
!! AGATACCGAAAAGTTTTATG+AGG - chr4.2:38560618-38560637 MS.gene003882:CDS 30.0%
!!! ACTTTTAGTTCAGATGATGA+TGG - chr4.2:38560484-38560503 MS.gene003882:CDS 30.0%
!!! CCAAGTAAAAGTGCTAATTT+TGG + chr4.2:38561338-38561357 None:intergenic 30.0%
!!! TTTTAAAACATAGGCTCTGA+AGG - chr4.2:38560770-38560789 MS.gene003882:CDS 30.0%
AAAAGTTCTCTCGTTCTTCT+CGG - chr4.2:38559730-38559749 MS.gene003882:intron 35.0%
ACATACGGTTGTTATGTACT+TGG - chr4.2:38558600-38558619 MS.gene003882:CDS 35.0%
AGAGTGCATACATTAATTCC+AGG + chr4.2:38561164-38561183 None:intergenic 35.0%
AGCTAATGTAGGACTATCTA+CGG + chr4.2:38559227-38559246 None:intergenic 35.0%
ATCAAAACAGTCCTTACGAA+AGG - chr4.2:38558697-38558716 MS.gene003882:intron 35.0%
ATGCAATTCCCATTCTTTCA+GGG - chr4.2:38560564-38560583 MS.gene003882:intron 35.0%
CACATCATTTGCGTATTTGA+AGG - chr4.2:38558841-38558860 MS.gene003882:intron 35.0%
GCATTAGTGGTAGAAATTTG+TGG + chr4.2:38561101-38561120 None:intergenic 35.0%
GCCTACTAATAACAATTGCT+AGG + chr4.2:38559988-38560007 None:intergenic 35.0%
GCTATTAATAGTCGTTCATC+AGG - chr4.2:38560906-38560925 MS.gene003882:CDS 35.0%
TATGCAATTCCCATTCTTTC+AGG - chr4.2:38560563-38560582 MS.gene003882:intron 35.0%
TCGTTGTATGTGATTGAGTT+AGG - chr4.2:38560187-38560206 MS.gene003882:intron 35.0%
TCTTTATCTACTCCTTGATG+TGG - chr4.2:38560997-38561016 MS.gene003882:intron 35.0%
TTCTACCACTAATGCAAATC+AGG - chr4.2:38561106-38561125 MS.gene003882:intron 35.0%
TTGCTAGGAATTGTAACACA+AGG + chr4.2:38559973-38559992 None:intergenic 35.0%
TTGTTTCCCTATGCAGAAAA+AGG - chr4.2:38560038-38560057 MS.gene003882:intron 35.0%
TTTCTGCATAGGGAAACAAA+TGG + chr4.2:38560037-38560056 None:intergenic 35.0%
! AACGTTTACTTCTTTTCCTC+AGG + chr4.2:38559272-38559291 None:intergenic 35.0%
! AAGTATTTTCATTCCCACAG+TGG - chr4.2:38559834-38559853 MS.gene003882:CDS 35.0%
! ACTTGAAGAAAATGATGACG+AGG - chr4.2:38559572-38559591 MS.gene003882:CDS 35.0%
! AGAAGAACGAGAGAACTTTT+TGG + chr4.2:38559730-38559749 None:intergenic 35.0%
! GAAGAACGAGAGAACTTTTT+GGG + chr4.2:38559729-38559748 None:intergenic 35.0%
! GTCTTTTCTGGATTTAGGAA+TGG - chr4.2:38560708-38560727 MS.gene003882:CDS 35.0%
! TTCTACCTCTTGTACTTTTC+CGG + chr4.2:38559149-38559168 None:intergenic 35.0%
!!! CACTTTTGAAGCCAACATTT+AGG + chr4.2:38559069-38559088 None:intergenic 35.0%
!!! GACTGTTTTGATTTGACCTT+CGG + chr4.2:38558689-38558708 None:intergenic 35.0%
AATTCACTGCACATCTTACG+TGG - chr4.2:38560342-38560361 MS.gene003882:intron 40.0%
ACAAGTCACCTAGGGTAAAA+TGG - chr4.2:38560587-38560606 MS.gene003882:intron 40.0%
CGTAAAAGATCATCTGTTGC+TGG - chr4.2:38559414-38559433 MS.gene003882:intron 40.0%
CTCTTGTAATCTGAATGTGC+AGG - chr4.2:38561655-38561674 MS.gene003882:CDS 40.0%
CTGCTGGATGATTATGAACT+TGG - chr4.2:38559865-38559884 MS.gene003882:CDS 40.0%
TAATCTGAATGTGCAGGAAG+AGG - chr4.2:38561661-38561680 MS.gene003882:CDS 40.0%
TAGTTGAGAATACCTACCTC+CGG + chr4.2:38559946-38559965 None:intergenic 40.0%
TCACTATCATCAGATTGCTC+TGG + chr4.2:38561752-38561771 None:intergenic 40.0%
TGCTGATGTTAAAGATCAGG+AGG - chr4.2:38561706-38561725 MS.gene003882:intron 40.0%
TTACAGCAAGATGTTACCTG+AGG - chr4.2:38559253-38559272 MS.gene003882:CDS 40.0%
TTACTTGTCTGTGCTCTTCA+TGG - chr4.2:38560942-38560961 MS.gene003882:CDS 40.0%
TTGCAAGACAGCCTAAATGT+TGG - chr4.2:38559055-38559074 MS.gene003882:intron 40.0%
TTTCCCTATGCAGAAAAAGG+AGG - chr4.2:38560041-38560060 MS.gene003882:intron 40.0%
! AAAATGGATTCTCAAGGGAG+CGG + chr4.2:38561453-38561472 None:intergenic 40.0%
! CTCAGGAATGGATTTAGTGA+CGG + chr4.2:38558792-38558811 None:intergenic 40.0%
! GTGATGATGACTTACGGAAA+TGG - chr4.2:38561768-38561787 MS.gene003882:intron 40.0%
! TACTAGCTTGATCATCCTCT+TGG + chr4.2:38560519-38560538 None:intergenic 40.0%
! TACTTCCGGAAAAGTACAAG+AGG - chr4.2:38559141-38559160 MS.gene003882:intron 40.0%
! TGACTTGTCCCTGAAAGAAT+GGG + chr4.2:38560575-38560594 None:intergenic 40.0%
! TGTTTCGACCATTTTACCCT+AGG + chr4.2:38560598-38560617 None:intergenic 40.0%
! TTGCCTCCTTTTTCTGCATA+GGG + chr4.2:38560047-38560066 None:intergenic 40.0%
! TTTGCCTCCTTTTTCTGCAT+AGG + chr4.2:38560048-38560067 None:intergenic 40.0%
!! CAGTGCTGATGTTAAAGATC+AGG - chr4.2:38561703-38561722 MS.gene003882:intron 40.0%
AAGATCATCTGTTGCTGGTG+TGG - chr4.2:38559419-38559438 MS.gene003882:intron 45.0%
ACTACTGAAGACATGTCACC+TGG - chr4.2:38561143-38561162 MS.gene003882:intron 45.0%
ATGACCGTGGTAAAAGCTCA+AGG - chr4.2:38559445-38559464 MS.gene003882:intron 45.0%
CAAAGTCTTCCACCAATCGA+AGG - chr4.2:38560064-38560083 MS.gene003882:intron 45.0%
CCTGAGACCAACCTCAATAA+TGG - chr4.2:38558806-38558825 MS.gene003882:intron 45.0%
GGCGATTTCTTTCATGATGC+TGG - chr4.2:38560442-38560461 MS.gene003882:CDS 45.0%
GTCTTCAGTAGTGTCTTCAG+TGG + chr4.2:38561135-38561154 None:intergenic 45.0%
TAAAGATCAGGAGGATCCCA+TGG - chr4.2:38561715-38561734 MS.gene003882:intron 45.0%
TCTCGGCGAAGAAAAAGACA+AGG - chr4.2:38559747-38559766 MS.gene003882:CDS 45.0%
TCTGGACTATGATACGCCAT+GGG + chr4.2:38561734-38561753 None:intergenic 45.0%
TGATGGTTGTAGAGACCAAG+AGG - chr4.2:38560501-38560520 MS.gene003882:CDS 45.0%
TGCAGAAAATAAGGAGCGAC+CGG - chr4.2:38559924-38559943 MS.gene003882:CDS 45.0%
TGTCAAGGAATGCAGTGGAT+GGG + chr4.2:38559006-38559025 None:intergenic 45.0%
TTTCAGGGACAAGTCACCTA+GGG - chr4.2:38560579-38560598 MS.gene003882:intron 45.0%
! AAATGATGACGAGGACGAAG+TGG - chr4.2:38559581-38559600 MS.gene003882:intron 45.0%
! AAATGGATTCTCAAGGGAGC+GGG + chr4.2:38561452-38561471 None:intergenic 45.0%
! AATGGATTTAGTGACGGTGG+GGG + chr4.2:38558786-38558805 None:intergenic 45.0%
! AGGAATGGATTTAGTGACGG+TGG + chr4.2:38558789-38558808 None:intergenic 45.0%
! GAATGGATTTAGTGACGGTG+GGG + chr4.2:38558787-38558806 None:intergenic 45.0%
! GCACACTTTTGTTGCTGTCA+AGG + chr4.2:38559021-38559040 None:intergenic 45.0%
! GGAATGGATTTAGTGACGGT+GGG + chr4.2:38558788-38558807 None:intergenic 45.0%
! GGCTTCCTGATTTGCATTAG+TGG + chr4.2:38561114-38561133 None:intergenic 45.0%
! GTGACTTGTCCCTGAAAGAA+TGG + chr4.2:38560576-38560595 None:intergenic 45.0%
! TGCTGGTGCTAATAGTGAAG+AGG - chr4.2:38560459-38560478 MS.gene003882:CDS 45.0%
! TGTTGCTTACCTTCGATTGG+TGG + chr4.2:38560076-38560095 None:intergenic 45.0%
!! ACATAGGCTCTGAAGGAGTT+TGG - chr4.2:38560777-38560796 MS.gene003882:CDS 45.0%
!! ATTGAGGTTGGTCTCAGGAA+TGG + chr4.2:38558804-38558823 None:intergenic 45.0%
!! CCATTATTGAGGTTGGTCTC+AGG + chr4.2:38558809-38558828 None:intergenic 45.0%
!! CTGTGTTGCTTACCTTCGAT+TGG + chr4.2:38560079-38560098 None:intergenic 45.0%
!! GAACGAGAGAACTTTTTGGG+TGG + chr4.2:38559726-38559745 None:intergenic 45.0%
!! GTTGGCTTCAAAAGTGCTTC+AGG - chr4.2:38559073-38559092 MS.gene003882:intron 45.0%
!!! TTTTTTTTTTTTTATACATA+CGG - chr4.2:38558585-38558604 MS.gene003882:CDS 5.0%
AGAAAATAAGGAGCGACCGG+AGG - chr4.2:38559927-38559946 MS.gene003882:intron 50.0%
AGAGGTTGCAACAACTGAGG+AGG - chr4.2:38561679-38561698 MS.gene003882:CDS 50.0%
ATCATCTGTTGCTGGTGTGG+AGG - chr4.2:38559422-38559441 MS.gene003882:intron 50.0%
CATAATCATCCAGCAGAGCC+TGG + chr4.2:38559861-38559880 None:intergenic 50.0%
CATGCTACCTCGTCTGTAGA+TGG - chr4.2:38558737-38558756 MS.gene003882:intron 50.0%
CTCTGGACTATGATACGCCA+TGG + chr4.2:38561735-38561754 None:intergenic 50.0%
CTGTCAAGGAATGCAGTGGA+TGG + chr4.2:38559007-38559026 None:intergenic 50.0%
CTTTCAGGGACAAGTCACCT+AGG - chr4.2:38560578-38560597 MS.gene003882:intron 50.0%
GCTCTTTCCATCTACAGACG+AGG + chr4.2:38558747-38558766 None:intergenic 50.0%
GGAAGAGGTTGCAACAACTG+AGG - chr4.2:38561676-38561695 MS.gene003882:CDS 50.0%
GTCAAGGAATGCAGTGGATG+GGG + chr4.2:38559005-38559024 None:intergenic 50.0%
GTTGCTGTCAAGGAATGCAG+TGG + chr4.2:38559011-38559030 None:intergenic 50.0%
TTTCATTCCCACAGTGGACC+AGG - chr4.2:38559840-38559859 MS.gene003882:CDS 50.0%
TTTGACCTTCGGCGCAAACT+TGG + chr4.2:38558678-38558697 None:intergenic 50.0%
! AATGGATTCTCAAGGGAGCG+GGG + chr4.2:38561451-38561470 None:intergenic 50.0%
! GCTGCCTTGAGCTTTTACCA+CGG + chr4.2:38559452-38559471 None:intergenic 50.0%
AATAAGGAGCGACCGGAGGT+AGG - chr4.2:38559931-38559950 MS.gene003882:intron 55.0%
AGGAATGCAGTGGATGGGGA+AGG + chr4.2:38559001-38559020 None:intergenic 55.0%
ATGGATTCTCAAGGGAGCGG+GGG + chr4.2:38561450-38561469 None:intergenic 55.0%
TAGTGCCAAGTTTGCGCCGA+AGG - chr4.2:38558670-38558689 MS.gene003882:CDS 55.0%
TTCGGCGCAAACTTGGCACT+AGG + chr4.2:38558671-38558690 None:intergenic 55.0%
AGCAGAGCCTGGTCCACTGT+GGG + chr4.2:38559850-38559869 None:intergenic 60.0%
CACAGTGGACCAGGCTCTGC+TGG - chr4.2:38559849-38559868 MS.gene003882:CDS 65.0%
CAGCAGAGCCTGGTCCACTG+TGG + chr4.2:38559851-38559870 None:intergenic 65.0%
GCTGGTGTGGAGGATGACCG+TGG - chr4.2:38559432-38559451 MS.gene003882:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 38558567 38561814 38558567 ID=MS.gene003882
chr4.2 mRNA 38558567 38561814 38558567 ID=MS.gene003882.t1;Parent=MS.gene003882
chr4.2 exon 38561808 38561814 38561808 ID=MS.gene003882.t1.exon1;Parent=MS.gene003882.t1
chr4.2 CDS 38561808 38561814 38561808 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38561554 38561718 38561554 ID=MS.gene003882.t1.exon2;Parent=MS.gene003882.t1
chr4.2 CDS 38561554 38561718 38561554 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38561219 38561412 38561219 ID=MS.gene003882.t1.exon3;Parent=MS.gene003882.t1
chr4.2 CDS 38561219 38561412 38561219 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38560613 38561000 38560613 ID=MS.gene003882.t1.exon4;Parent=MS.gene003882.t1
chr4.2 CDS 38560613 38561000 38560613 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38560433 38560527 38560433 ID=MS.gene003882.t1.exon5;Parent=MS.gene003882.t1
chr4.2 CDS 38560433 38560527 38560433 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38560296 38560327 38560296 ID=MS.gene003882.t1.exon6;Parent=MS.gene003882.t1
chr4.2 CDS 38560296 38560327 38560296 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38559742 38559943 38559742 ID=MS.gene003882.t1.exon7;Parent=MS.gene003882.t1
chr4.2 CDS 38559742 38559943 38559742 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38559454 38559598 38559454 ID=MS.gene003882.t1.exon8;Parent=MS.gene003882.t1
chr4.2 CDS 38559454 38559598 38559454 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38559209 38559318 38559209 ID=MS.gene003882.t1.exon9;Parent=MS.gene003882.t1
chr4.2 CDS 38559209 38559318 38559209 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
chr4.2 exon 38558567 38558704 38558567 ID=MS.gene003882.t1.exon10;Parent=MS.gene003882.t1
chr4.2 CDS 38558567 38558704 38558567 ID=cds.MS.gene003882.t1;Parent=MS.gene003882.t1
Gene Sequence

>MS.gene003882

ATGGGATGTAATTTAATTTTTTTTTTTTTTTATACATACGGTTGTTATGTACTTGGATTATAATTATAATATAATATAATTATATTCTTTTTGTAGCTCGGCCTAGTGCCAAGTTTGCGCCGAAGGTCAAATCAAAACAGTCCTTACGAAAGGAAATATCTGAATCAGAACATGCTACCTCGTCTGTAGATGGAAAGAGCATGCATGTTGCTTCAACCCCCACCGTCACTAAATCCATTCCTGAGACCAACCTCAATAATGGTAATTATGTTTGCACATCATTTGCGTATTTGAAGGTTTTTTTTTTTCTCAAGCTCAATCTTGCACTAGTGTCACAATGCGTCAGACATTGAATCATTTCATATCTAGCATTGACAACTATTAAATGTGGTCTCTTATTAGATGGTGCAGAGACTGAATTGAATAAAGTGCCTTCCCCATCCACTGCATTCCTTGACAGCAACAAAAGTGTGCAAGTTAATAACTCATTGCAAGACAGCCTAAATGTTGGCTTCAAAAGTGCTTCAGGCGACAATAGCACTGCAATTTCCGAGAACAACATTCATTCCATTTTTACTTCCGGAAAAGTACAAGAGGTAGAATAGTTTATCAAATTATATTAATATTTTTCATGCTCTGCAGAAGAGTTTACAAACTCCGTAGATAGTCCTACATTAGCTGATTTTTTACAGCAAGATGTTACCTGAGGAAAAGAAGTAAACGTTAATTATTATGATTTGTCATGCTTTTTCACTTCTCTTAATTCTGAATTTGATTGTAGATGATTTTTCATTTCATTTGTAATTTTTATTAGGATGCTAATGAGAGGAAAAAATCTCTAAGGAAGCGTAAAAGATCATCTGTTGCTGGTGTGGAGGATGACCGTGGTAAAAGCTCAAGGCAGCTGAGAAAACAAGCAGCTTGTGAACATGTTAAAAAGTCAAACTCATTGATTGAGGATGATGATGAACTCGACCCTCCTTATATTTGTAACAATATAGATCAACTTGAAGAAAATGATGACGAGGACGAAGTGGATAATTCATCTAAGAAGAAAAGAGCAGTAGCAAGTTCAAAAAAGAAATATGTGGCTAAAAATGGAAAAGCATACAAAAAAAGCAAGGAAAATGGCGATTCAGAAAAAACTACTAAAGAACCACCCAAAAAGTTCTCTCGTTCTTCTCGGCGAAGAAAAAGACAAGGTAAACTAATCAATCTATTTGTTTAATTATCATTTTAATGACTATATTTTATGTGCTTATTGTGTAAGTATTTTCATTCCCACAGTGGACCAGGCTCTGCTGGATGATTATGAACTTGGTTCTCAATCAATTTCTCTTAGGGATATTCTTGCAATTGCAGAAAATAAGGAGCGACCGGAGGTAGGTATTCTCAACTATATTCCTTGTGTTACAATTCCTAGCAATTGTTATTAGTAGGCTTATGGCTTAAATTTCACTAACTGTTCCATTTGTTTCCCTATGCAGAAAAAGGAGGCAAAGTCTTCCACCAATCGAAGGTAAGCAACACAGCTCGACAGTGTATTATCAAAGCAGTATTGGAGTATATTTAGTGTAGCTAATTGTTAGTAGCCTAAGAAGTTAAGATATATGTGTATATTCGTTGTATGTGATTGAGTTAGGTAGAATCTTCTACCCTTTGTTAGCTTTCACGATTTATTTCCTTGTCAATTATATTTTTTTAATATGAAAATATATATCTTGTTGAAAATCTGCGTATAGCGCAGTAAACATCTTTCTTGCTTTATTTACTGCAATTCACTGCACATCTTACGTGGTTCCAAACATCTTATTTACCATCATCCAGTAGTAAATAGATTTATATTTGTATTTTAAAATATTTATCTGCAGTGGTGGCGATTTCTTTCATGATGCTGGTGCTAATAGTGAAGAGGAGACTTTTAGTTCAGATGATGATGGTTGTAGAGACCAAGAGGATGATCAAGCTAGTAAAAAGTTTGCATCAACTGTCGCTTTATGCAATTCCCATTCTTTCAGGGACAAGTCACCTAGGGTAAAATGGTCGAAACAAGATACCGAAAAGTTTTATGAGGTATTCTTCACATGATTCTTACAATATGTCGTTATTATTTCTTTAAACACAATTATATTACTAATTTTGTCTTTTCTGGATTTAGGAATGGAGACAGATTAGTCATTTTTAATAAAATAACTGTTCATTTTTTTAAAACATAGGCTCTGAAGGAGTTTGGCCTAGATTTTACAATGATTCAACAAATTTTTTCTGGTAAATCAAGACGTCAAATTATGCTGAAGTTCAAGAATGAAGACCGACATCATCCTTTACGAATAACTGATGCTATTAATAGTCGTTCATCAGGTTAAACTCTTCAGTTACTTGTCTGTGCTCTTCATGGTCATATAGTACATGATCAGTAGGGTTTATTGTTCTTTATCTACTCCTTGATGTGGAATAACATTTTACTGAATGAAATCTATAATCTTCTCTCTTTAGATCATCATATTTTTAAGTCGGTGATTGCGAAACTGCCACAAATTTCTACCACTAATGCAAATCAGGAAGCCACTGAAGACACTACTGAAGACATGTCACCTGGAATTAATGTATGCACTCTTAGCTTGTTTTGTTTTATATTTTGAGGTCTATATTCATTTGGTACACATATAGTTTCTGCTAATTTCTTTTAAATCTCATTTATTTATCAGAGACATATTCTTATTGCATGATATTTGTGAGTTAAAATTTGGAATATAATGATCTTTCAACCAAAATTAGCACTTTTACTTGGTTCAGTCCTTCGTTACTCAAATTTACTTCCATGTAATTTAAACCCTAATATAGAAGCAGCGTATATAGATTTGTGATTGAGATGTTATTCCCCCGCTCCCTTGAGAATCCATTTTATACAAGGGAACAACACTTCACCATCATGTATATGTGATATGACGTAAAAACTTTCAAGGCATATCCTAGCACAATAAAGACATATATTTACCACTTCATTTATAGATTTAGAATATCTTAAACTAAATCCACATAATTGTAATATTTCTTATTATCTAATTGACCTTGCAAATATTCAAGACTCTTGTAATCTGAATGTGCAGGAAGAGGTTGCAACAACTGAGGAGGACAGTGCTGATGTTAAAGATCAGGAGGATCCCATGGCGTATCATAGTCCAGAGCAATCTGATGATAGTGATGATGACTTACGGAAATGGTCTCAGTATCAAAGTGTTATTTAG
Protein sequence

>MS.gene003882.t1

MGSRPSAKFAPKVKSKQSLRKEISESEHATSSVDGKSMHVASTPTVTKSIPETNLNNDGAETELNKVPSPSTAFLDSNKSVQVNNSLQDSLNVGFKSASGDNSTAISENNIHSIFTSGKVQEDANERKKSLRKRKRSSVAGVEDDRGKSSRQLRKQAACEHVKKSNSLIEDDDELDPPYICNNIDQLEENDDEDEVDNSSKKKRAVASSKKKYVAKNGKAYKKSKENGDSEKTTKEPPKKFSRSSRRRKRQVDQALLDDYELGSQSISLRDILAIAENKERPEKKEAKSSTNRSGGDFFHDAGANSEEETFSSDDDGCRDQEDDQASKKFASTVALCNSHSFRDKSPRVKWSKQDTEKFYEALKEFGLDFTMIQQIFSGKSRRQIMLKFKNEDRHHPLRITDAINSRSSDHHIFKSVIAKLPQISTTNANQEATEDTTEDMSPGINEEVATTEEDSADVKDQEDPMAYHSPEQSDDSDDDLRKWSQYQSVI