AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004025

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004025.t1 MTR_4g064150 94.966 735 37 0 1 735 1 735 0.0 1418
MS.gene004025.t1 MTR_4g064120 71.988 664 136 7 73 735 14 628 0.0 931
MS.gene004025.t1 MTR_4g064180 71.687 664 138 8 73 735 14 628 0.0 919
MS.gene004025.t1 MTR_2g097410 56.051 785 253 18 1 735 1 743 0.0 813
MS.gene004025.t1 MTR_4g064160 49.135 694 279 14 83 735 26 686 0.0 606
MS.gene004025.t1 MTR_7g062120 45.007 691 330 17 83 735 64 742 0.0 543
MS.gene004025.t1 MTR_2g097467 44.461 668 297 18 86 735 45 656 1.14e-174 515
MS.gene004025.t1 MTR_4g064200 53.082 503 213 9 248 735 158 652 1.08e-173 513
MS.gene004025.t1 MTR_2g097473 44.444 666 300 17 86 735 45 656 3.00e-171 507
MS.gene004025.t1 MTR_2g097463 43.297 649 312 13 96 735 56 657 1.16e-166 495
MS.gene004025.t1 MTR_2g097310 48.117 478 231 7 260 735 178 640 1.11e-156 469
MS.gene004025.t1 MTR_2g097350 50.342 439 202 7 299 735 218 642 3.57e-150 452
MS.gene004025.t1 MTR_2g097390 47.598 458 221 8 283 735 200 643 1.35e-147 446
MS.gene004025.t1 MTR_2g097380 45.295 457 189 9 283 735 164 563 8.44e-131 399
MS.gene004025.t1 MTR_2g082090 32.713 376 248 4 357 732 208 578 6.84e-67 231
MS.gene004025.t1 MTR_5g097480 31.915 376 251 3 357 732 173 543 2.60e-62 218
MS.gene004025.t1 MTR_3g056110 32.540 378 246 6 357 732 171 541 9.95e-60 211
MS.gene004025.t1 MTR_2g026250 29.630 378 260 4 356 732 223 595 5.76e-58 207
MS.gene004025.t1 MTR_4g133660 30.570 386 248 8 356 733 170 543 2.26e-56 202
MS.gene004025.t1 MTR_5g094450 31.606 386 243 9 357 732 157 531 1.22e-55 199
MS.gene004025.t1 MTR_8g442410 30.250 400 243 7 353 735 147 527 4.17e-53 192
MS.gene004025.t1 MTR_1g029420 28.610 374 262 3 359 732 223 591 1.69e-52 192
MS.gene004025.t1 MTR_7g027190 31.034 406 233 8 351 732 290 672 1.75e-52 193
MS.gene004025.t1 MTR_3g089055 28.610 374 262 3 359 732 196 564 2.30e-52 191
MS.gene004025.t1 MTR_3g089055 28.610 374 262 3 359 732 201 569 2.36e-52 191
MS.gene004025.t1 MTR_4g104020 29.648 398 249 8 350 732 138 519 4.97e-52 189
MS.gene004025.t1 MTR_3g065980 30.193 414 252 8 336 732 146 539 3.47e-49 182
MS.gene004025.t1 MTR_7g074650 30.000 370 241 7 365 734 442 793 1.64e-45 175
MS.gene004025.t1 MTR_4g076140 27.074 458 262 9 337 732 21 468 3.56e-44 166
MS.gene004025.t1 MTR_2g034260 29.648 398 250 10 351 732 200 583 2.98e-43 166
MS.gene004025.t1 MTR_2g034250 29.620 395 255 9 351 732 200 584 4.69e-42 162
MS.gene004025.t1 MTR_5g009080 27.126 435 247 10 358 732 53 477 4.38e-40 154
MS.gene004025.t1 MTR_3g022830 26.128 421 250 8 362 732 26 435 1.28e-39 152
MS.gene004025.t1 MTR_4g102790 26.546 388 263 10 357 734 288 663 1.22e-38 153
MS.gene004025.t1 MTR_4g076020 26.954 371 244 5 365 735 88 431 3.22e-38 148
MS.gene004025.t1 MTR_4g097080 28.052 385 257 10 359 734 131 504 5.72e-37 145
MS.gene004025.t1 MTR_2g089100 26.406 409 258 9 358 735 49 445 1.12e-36 144
MS.gene004025.t1 MTR_1g069725 27.763 389 245 14 353 732 101 462 5.15e-36 142
MS.gene004025.t1 MTR_2g034280 27.735 393 262 9 351 732 193 574 3.80e-35 141
MS.gene004025.t1 MTR_1g096030 30.000 400 237 14 358 732 77 458 1.25e-33 135
MS.gene004025.t1 MTR_3g072710 26.551 403 264 10 350 732 109 499 1.94e-32 132
MS.gene004025.t1 MTR_5g015490 25.654 382 274 5 360 734 113 491 1.69e-31 129
MS.gene004025.t1 MTR_4g077760 26.923 364 240 9 377 732 209 554 2.42e-31 129
MS.gene004025.t1 MTR_5g015950 23.709 426 268 11 358 735 32 448 1.62e-30 125
MS.gene004025.t1 MTR_3g053270 23.709 426 268 11 358 735 32 448 1.62e-30 125
MS.gene004025.t1 MTR_7g109580 28.205 390 257 12 351 732 179 553 1.70e-30 127
MS.gene004025.t1 MTR_4g095500 24.582 419 271 9 358 735 56 470 2.34e-30 125
MS.gene004025.t1 MTR_5g058860 28.463 397 241 14 362 733 119 497 7.87e-30 124
MS.gene004025.t1 MTR_7g057230 24.793 363 252 7 377 733 310 657 1.75e-29 125
MS.gene004025.t1 MTR_6g047750 26.031 388 254 12 359 732 293 661 1.11e-28 122
MS.gene004025.t1 MTR_1g086970 26.185 401 258 13 351 732 96 477 6.68e-28 118
MS.gene004025.t1 MTR_8g093070 26.650 409 247 17 346 735 124 498 3.48e-27 116
MS.gene004025.t1 MTR_2g099110 25.667 300 213 4 361 659 74 364 1.15e-21 99.4
MS.gene004025.t1 MTR_0092s0100 22.222 396 287 7 338 732 355 730 2.00e-14 77.4
MS.gene004025.t1 MTR_0092s0100 22.222 396 287 7 338 732 352 727 2.32e-14 77.4
MS.gene004025.t1 MTR_4g026485 27.536 276 180 7 362 633 270 529 2.57e-14 77.0
MS.gene004025.t1 MTR_8g020840 24.093 386 234 14 361 719 171 524 1.04e-13 75.1
MS.gene004025.t1 MTR_3g087300 44.000 75 32 2 316 380 134 208 6.59e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004025.t1 AT1G07530 47.143 700 290 17 96 735 88 767 0.0 576
MS.gene004025.t1 AT2G29060 47.641 657 281 15 88 734 53 656 0.0 560
MS.gene004025.t1 AT2G29060 47.455 668 303 14 88 734 53 693 0.0 556
MS.gene004025.t1 AT2G29065 45.622 651 284 15 96 735 39 630 0.0 531
MS.gene004025.t1 AT1G07520 46.142 648 318 11 96 735 71 695 3.72e-179 528
MS.gene004025.t1 AT1G07520 46.142 648 318 11 96 735 72 696 4.05e-179 528
MS.gene004025.t1 AT1G07520 50.947 528 241 8 214 735 16 531 8.67e-178 519
MS.gene004025.t1 AT1G07520 50.947 528 241 8 214 735 16 531 8.67e-178 519
MS.gene004025.t1 AT2G37650 43.491 676 327 18 83 734 58 702 1.74e-166 496
MS.gene004025.t1 AT2G37650 43.491 676 327 18 83 734 70 714 1.88e-166 496
MS.gene004025.t1 AT3G46600 49.889 451 209 5 285 735 148 581 2.34e-151 453
MS.gene004025.t1 AT3G46600 49.889 451 209 5 285 735 116 549 2.87e-151 452
MS.gene004025.t1 AT5G59450 47.500 480 233 7 264 735 132 600 8.12e-148 445
MS.gene004025.t1 AT3G46600 52.646 378 174 2 358 735 1 373 2.34e-140 417
MS.gene004025.t1 AT2G04890 31.915 376 244 4 357 732 49 412 2.13e-59 206
MS.gene004025.t1 AT1G50600 31.915 376 251 3 357 732 155 525 4.43e-59 209
MS.gene004025.t1 AT1G50600 31.915 376 251 3 357 732 226 596 2.46e-58 208
MS.gene004025.t1 AT1G50600 31.915 376 251 3 357 732 230 600 3.23e-58 208
MS.gene004025.t1 AT5G48150 30.319 376 258 3 357 732 118 489 1.36e-56 201
MS.gene004025.t1 AT5G48150 30.319 376 258 3 357 732 118 489 1.36e-56 201
MS.gene004025.t1 AT5G48150 30.319 376 258 3 357 732 118 489 1.36e-56 201
MS.gene004025.t1 AT5G48150 30.319 376 258 3 357 732 118 489 1.36e-56 201
MS.gene004025.t1 AT1G21450 30.319 376 254 5 359 732 223 592 4.48e-54 196
MS.gene004025.t1 AT4G17230 30.676 414 277 5 325 734 119 526 4.73e-52 189
MS.gene004025.t1 AT4G17230 30.676 414 277 5 325 734 119 526 4.73e-52 189
MS.gene004025.t1 AT1G66350 30.971 381 234 10 356 732 150 505 3.54e-47 175
MS.gene004025.t1 AT1G50420 25.463 432 258 5 358 732 54 478 5.33e-46 171
MS.gene004025.t1 AT2G01570 29.948 384 240 10 356 732 219 580 7.98e-46 173
MS.gene004025.t1 AT3G54220 30.025 393 258 8 343 734 275 651 9.13e-46 174
MS.gene004025.t1 AT3G03450 28.460 383 252 9 356 732 178 544 2.87e-45 171
MS.gene004025.t1 AT1G14920 30.105 382 242 9 356 732 167 528 3.10e-45 170
MS.gene004025.t1 AT5G17490 25.952 420 281 10 320 732 119 515 1.32e-41 159
MS.gene004025.t1 AT5G41920 28.084 381 253 6 355 732 37 399 1.03e-40 154
MS.gene004025.t1 AT5G66770 26.667 405 279 8 333 732 192 583 2.12e-36 145
MS.gene004025.t1 AT5G52510 26.154 390 256 10 359 732 266 639 1.17e-35 144
MS.gene004025.t1 AT1G63100 30.931 333 216 8 357 686 273 594 1.56e-35 143
MS.gene004025.t1 AT1G63100 30.931 333 216 8 357 686 248 569 1.74e-35 143
MS.gene004025.t1 AT4G37650 25.191 393 269 10 360 734 145 530 1.46e-33 136
MS.gene004025.t1 AT3G50650 28.972 321 209 5 370 684 184 491 9.49e-33 134
MS.gene004025.t1 AT3G49950 26.854 391 264 10 358 734 27 409 8.59e-31 125
MS.gene004025.t1 AT1G55580 24.821 419 256 11 358 732 41 444 5.13e-25 109
MS.gene004025.t1 AT4G08250 26.448 397 262 14 351 732 95 476 9.61e-25 108
MS.gene004025.t1 AT3G13840 23.023 430 255 16 331 732 128 509 4.42e-14 75.9

Find 163 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAATTAATGGACAAATTT+AGG 0.087513 4.3:-39611157 MS.gene004025:CDS
GATGAGATTTAAGAATTTAT+TGG 0.142030 4.3:-39611528 MS.gene004025:CDS
ACCCTCCATATCATTGATTT+TGG 0.161875 4.3:-39611824 MS.gene004025:CDS
TCCATTGAATTCGGAATTAA+TGG 0.174492 4.3:-39611168 MS.gene004025:CDS
AATCAATATGATAGCTTTCT+TGG 0.191887 4.3:-39613066 MS.gene004025:CDS
ATGCCATACCATCGCGTTAT+TGG 0.192133 4.3:-39611613 MS.gene004025:CDS
CATTCTTTGCTACACGGTTT+AGG 0.223208 4.3:-39611397 MS.gene004025:CDS
AAATGCAGAACCGCATTTCT+AGG 0.241890 4.3:+39611482 None:intergenic
CTCCCTAACCATATCCAAAT+TGG 0.268685 4.3:+39612471 None:intergenic
TTTGTTCAGTCAATTGTTAA+TGG 0.288838 4.3:-39611434 MS.gene004025:CDS
ATCAGAGTTATGAATATCTA+AGG 0.292408 4.3:+39612801 None:intergenic
TGAATCATCCAATTTGGATA+TGG 0.301149 4.3:-39612479 MS.gene004025:CDS
CAAACAGCTTCCATTGAATT+CGG 0.304055 4.3:-39611177 MS.gene004025:CDS
ATTCTTTGCTACACGGTTTA+GGG 0.306681 4.3:-39611396 MS.gene004025:CDS
TCACTAGCTTGAGGAAAGTT+TGG 0.320173 4.3:+39613219 None:intergenic
CATCCAATTTGGATATGGTT+AGG 0.330014 4.3:-39612474 MS.gene004025:CDS
TGCCATACCATCGCGTTATT+GGG 0.330847 4.3:-39611612 MS.gene004025:CDS
ACTCTATCCCGGTGATCTTC+AGG 0.333841 4.3:+39611732 None:intergenic
GGATACCAAAATCAATGATA+TGG 0.351602 4.3:+39611819 None:intergenic
CGAGTCTTCCCTCCATTTGA+TGG 0.357746 4.3:+39612214 None:intergenic
AGAAAGCTATCATATTGATT+TGG 0.360772 4.3:+39613069 None:intergenic
AGGAAGCTATCATATTGATT+TGG 0.360772 4.3:+39613135 None:intergenic
CTTCCCTATAGAAGGTTATA+TGG 0.365695 4.3:-39613013 MS.gene004025:CDS
TTTATATGCTTCCACATGTT+GGG 0.369926 4.3:-39611051 MS.gene004025:CDS
CATATAAACAGTGGCAGGTT+AGG 0.372839 4.3:-39611217 MS.gene004025:CDS
TGATCAGAAACAGGAGTATC+AGG 0.375983 4.3:+39612709 None:intergenic
ATTGCTGTTGCTGTTGCTGC+TGG 0.378194 4.3:+39612771 None:intergenic
GGCCTTGAGGCACGCCTTGT+CGG 0.379088 4.3:-39612013 MS.gene004025:CDS
ATTAGCATTCCTGTTGTCAT+TGG 0.384143 4.3:+39612114 None:intergenic
CGCCTTGTCGGCGATGGTAC+TGG 0.387590 4.3:-39612001 MS.gene004025:CDS
TAGTGATGGACTCAGAGATT+TGG 0.396758 4.3:-39612626 MS.gene004025:CDS
ATATCTAAGGGGTGTTGATT+AGG 0.398335 4.3:+39612814 None:intergenic
ATCCCGGTGATCTTCAGGTT+TGG 0.401423 4.3:+39611737 None:intergenic
GGGTGTTGATTAGGAGAAAG+AGG 0.402256 4.3:+39612823 None:intergenic
AGGAAGCTATCATATTCATT+TGG 0.404865 4.3:+39613102 None:intergenic
ATTATCAGAGATGTTTGATA+AGG 0.416031 4.3:-39612320 MS.gene004025:CDS
AGAGAATCCGTTTGTGTTGA+AGG 0.418700 4.3:-39612446 MS.gene004025:CDS
TCCATTAATTCCGAATTCAA+TGG 0.422266 4.3:+39611167 None:intergenic
TACTACCACTTTGGTGAAGA+AGG 0.425321 4.3:-39613183 MS.gene004025:CDS
GCCACTACCTTCCCTATAGA+AGG 0.428776 4.3:-39613021 MS.gene004025:CDS
AACAGGAGTATCAGGTGAAA+GGG 0.430936 4.3:+39612717 None:intergenic
GAGGCCATATAACCTTCTAT+AGG 0.431388 4.3:+39613009 None:intergenic
CTGCCACTGTTTATATGTCT+CGG 0.432575 4.3:+39611223 None:intergenic
ATGAGCTACTGAAGCAGATT+AGG 0.435797 4.3:-39612093 MS.gene004025:CDS
AGGCAACATTCTTCACCCTC+TGG 0.447707 4.3:-39612073 MS.gene004025:CDS
CTGTATATGCCAATGACAAC+AGG 0.450042 4.3:-39612123 MS.gene004025:CDS
ACAAACGGTTACTACCACTT+TGG 0.452669 4.3:-39613192 MS.gene004025:CDS
AGAATGAAACAATAGATTTG+AGG 0.453249 4.3:-39612171 MS.gene004025:CDS
GGCAACATTCTTCACCCTCT+GGG 0.453926 4.3:-39612072 MS.gene004025:CDS
ATGGAAAGAATCGAAGAAAC+TGG 0.455010 4.3:-39611686 MS.gene004025:CDS
ATTTCTCAACCTCATTCTAC+TGG 0.458162 4.3:-39612652 MS.gene004025:CDS
TTTCTTGGTGCTGATCCACT+TGG 0.458368 4.3:-39613051 MS.gene004025:CDS
GTTTCTTCGATTCTTTCCAT+TGG 0.459734 4.3:+39611689 None:intergenic
TTCAAGAAAAGTTTAGAAGA+AGG 0.460061 4.3:-39612544 MS.gene004025:CDS
AGGCCATATAACCTTCTATA+GGG 0.460382 4.3:+39613010 None:intergenic
ATCCAATTTGGATATGGTTA+GGG 0.463279 4.3:-39612473 MS.gene004025:CDS
TTTCTACCCTTCAACACAAA+CGG 0.464697 4.3:+39612439 None:intergenic
CAGTCATTTGTAAGCTAAGT+GGG 0.470257 4.3:+39612884 None:intergenic
GTGGTAACATTGCTGTAACT+TGG 0.472903 4.3:+39611133 None:intergenic
AGGTTATATGGCCTCGGCGA+CGG 0.474767 4.3:-39613001 MS.gene004025:CDS
GACAACAATTGGATGCTTCA+AGG 0.476004 4.3:-39611089 MS.gene004025:CDS
GGGATAGAGTTCCCTTTACC+TGG 0.477006 4.3:-39611719 MS.gene004025:CDS
GTAGAGATCCTCTTGGTGTT+CGG 0.479560 4.3:+39611956 None:intergenic
TGATTAACATGATCAGAAAC+AGG 0.483590 4.3:+39612700 None:intergenic
TGCCACTGTTTATATGTCTC+GGG 0.483709 4.3:+39611224 None:intergenic
CCGCAGCCTCAACTCTTGAC+TGG 0.485147 4.3:-39612505 MS.gene004025:CDS
GAGGAGTCACCTCCATCAAA+TGG 0.486751 4.3:-39612226 MS.gene004025:CDS
CAAGCTAGTGATTTCACAAA+CGG 0.489712 4.3:-39613207 MS.gene004025:CDS
GAATGAGGTTGAGAAATAGC+AGG 0.490215 4.3:+39612658 None:intergenic
TTGGCTCATTACTTTGCCAA+TGG 0.490767 4.3:-39612034 MS.gene004025:CDS
TTGGACGAAAACCAGGTAAA+GGG 0.491920 4.3:+39611708 None:intergenic
ACAATTGGATGCTTCAAGGT+TGG 0.492282 4.3:-39611085 MS.gene004025:CDS
AAGAACAAGAAGAAGAAGCT+TGG 0.493244 4.3:-39612387 MS.gene004025:CDS
CTTTCCATTGGACGAAAACC+AGG 0.495527 4.3:+39611701 None:intergenic
GAAAATGATTCCTTGCAGAA+TGG 0.503064 4.3:-39612262 MS.gene004025:CDS
TTGGCCACTGAAAACCATAT+AGG 0.503360 4.3:+39611798 None:intergenic
TGGGGATGGACCACAGAGGT+TGG 0.505585 4.3:-39612053 MS.gene004025:CDS
CCGGTGATCTTCAGGTTTGG+AGG 0.507786 4.3:+39611740 None:intergenic
TTCCCAATAACGCGATGGTA+TGG 0.510255 4.3:+39611610 None:intergenic
GCAGAATGGACAAGTGAAAA+CGG 0.510872 4.3:-39612248 MS.gene004025:CDS
AAGGCGTGCCTCAAGGCCAT+TGG 0.511609 4.3:+39612018 None:intergenic
TATAGAAGGTTATATGGCCT+CGG 0.515236 4.3:-39613007 MS.gene004025:CDS
CATTCTTCACCCTCTGGGGA+TGG 0.516297 4.3:-39612067 MS.gene004025:CDS
ATTCATTTGGATCAGCATCA+AGG 0.522321 4.3:+39613115 None:intergenic
TTGGGAAACAATTCGAGTTG+AGG 0.525214 4.3:-39611594 MS.gene004025:CDS
CGCCGACAAGGCGTGCCTCA+AGG 0.525318 4.3:+39612011 None:intergenic
CTAAACCGTGTAGCAAAGAA+TGG 0.525919 4.3:+39611398 None:intergenic
TCAGTCATTTGTAAGCTAAG+TGG 0.527232 4.3:+39612883 None:intergenic
GGATTCTTCTATTACACAGA+TGG 0.528072 4.3:-39612605 MS.gene004025:CDS
GGTATGGCATTAAACTCAAA+GGG 0.528598 4.3:+39611626 None:intergenic
ATTGGACGAAAACCAGGTAA+AGG 0.530391 4.3:+39611707 None:intergenic
CATTAAACTCAAAGGGAACA+TGG 0.533197 4.3:+39611633 None:intergenic
AATGAGGTTGAGAAATAGCA+GGG 0.534181 4.3:+39612659 None:intergenic
CGAGACATATAAACAGTGGC+AGG 0.537307 4.3:-39611222 MS.gene004025:CDS
AAATCAGTGTCTTCGAGGGA+TGG 0.542487 4.3:+39612976 None:intergenic
AAACAGGAGTATCAGGTGAA+AGG 0.542644 4.3:+39612716 None:intergenic
GTCAAGAGTTGAGGCTGCGG+AGG 0.544335 4.3:+39612508 None:intergenic
GAGAATCCGTTTGTGTTGAA+GGG 0.545933 4.3:-39612445 MS.gene004025:CDS
CTCCAGTACCATCGCCGACA+AGG 0.546196 4.3:+39611999 None:intergenic
TTAGATATTCATAACTCTGA+TGG 0.546938 4.3:-39612799 MS.gene004025:CDS
GTTCATAAACCAAATCCTGA+TGG 0.550468 4.3:-39612938 MS.gene004025:CDS
TACCACGCGAAAACAAATAC+AGG 0.550992 4.3:-39611325 MS.gene004025:CDS
TCAGAGTTATGAATATCTAA+GGG 0.551258 4.3:+39612802 None:intergenic
TACCAAAATCAATGATATGG+AGG 0.553791 4.3:+39611822 None:intergenic
CTCCAAACCTGAAGATCACC+GGG 0.554977 4.3:-39611739 MS.gene004025:CDS
TGGTATGGCATTAAACTCAA+AGG 0.555962 4.3:+39611625 None:intergenic
AGGTAAAGGGAACTCTATCC+CGG 0.560031 4.3:+39611721 None:intergenic
CCTCCAAACCTGAAGATCAC+CGG 0.561178 4.3:-39611740 MS.gene004025:CDS
GAGGCACGCCTTGTCGGCGA+TGG 0.563864 4.3:-39612007 MS.gene004025:CDS
ATGAACGTTGTAGCTTGTGA+AGG 0.570356 4.3:-39611266 MS.gene004025:CDS
CATTCTACTGGTGTTAGTGA+TGG 0.570556 4.3:-39612640 MS.gene004025:CDS
CCAGTCAAGAGTTGAGGCTG+CGG 0.571987 4.3:+39612505 None:intergenic
ATTCGCCATTCTTTGCTACA+CGG 0.575217 4.3:-39611403 MS.gene004025:CDS
CATATAACCTTCTATAGGGA+AGG 0.576430 4.3:+39613014 None:intergenic
TGGATGCTTCAAGGTTGGAA+AGG 0.577566 4.3:-39611080 MS.gene004025:CDS
TCTTGGTGTTCGGAGAGGAA+TGG 0.578017 4.3:+39611966 None:intergenic
CTCAGCAGTAGAGATCCTCT+TGG 0.580706 4.3:+39611949 None:intergenic
ACCTTCTATAGGGAAGGTAG+TGG 0.581330 4.3:+39613020 None:intergenic
GTGATGTGAGTTGATTATGC+TGG 0.583220 4.3:+39611026 None:intergenic
TACTCCCTTCTTCACCAAAG+TGG 0.586539 4.3:+39613178 None:intergenic
ACTACCACTTTGGTGAAGAA+GGG 0.587510 4.3:-39613182 MS.gene004025:CDS
CTTTGCTACACGGTTTAGGG+AGG 0.591419 4.3:-39611393 MS.gene004025:CDS
ATGGAAGCGCTTGCAATAGT+CGG 0.593097 4.3:+39611652 None:intergenic
TTACTTTGCCAATGGCCTTG+AGG 0.593344 4.3:-39612026 MS.gene004025:CDS
ATTGATTTGGATCAGCCTCA+AGG 0.595193 4.3:+39613082 None:intergenic
AAACAAATACAGGATGCTGA+TGG 0.598030 4.3:-39611315 MS.gene004025:CDS
CAATTTGGATATGGTTAGGG+AGG 0.598257 4.3:-39612470 MS.gene004025:CDS
CTCTGTGGTCCATCCCCAGA+GGG 0.598960 4.3:+39612058 None:intergenic
TTTCACTTGTCCATTCTGCA+AGG 0.603209 4.3:+39612252 None:intergenic
ACCAAAATCAATGATATGGA+GGG 0.610467 4.3:+39611823 None:intergenic
ATACAGGATGCTGATGGAGA+GGG 0.620955 4.3:-39611309 MS.gene004025:CDS
AAGCTAAGTGGGTCATAGAA+CGG 0.623038 4.3:+39612895 None:intergenic
ATTGTTTCCCAATAACGCGA+TGG 0.623674 4.3:+39611605 None:intergenic
GTCTTCCCTCCATTTGATGG+AGG 0.626646 4.3:+39612217 None:intergenic
GAGTCACCTCCATCAAATGG+AGG 0.628730 4.3:-39612223 MS.gene004025:CDS
TCACTAACACCAGTAGAATG+AGG 0.628786 4.3:+39612643 None:intergenic
TCAAAACCAGTCAAGAGTTG+AGG 0.630836 4.3:+39612499 None:intergenic
AATACAGGATGCTGATGGAG+AGG 0.637418 4.3:-39611310 MS.gene004025:CDS
ACAGGAGTATCAGGTGAAAG+GGG 0.642453 4.3:+39612718 None:intergenic
ATTCCTCTCCGAACACCAAG+AGG 0.643154 4.3:-39611964 MS.gene004025:CDS
AGTCACCTCCATCAAATGGA+GGG 0.643984 4.3:-39612222 MS.gene004025:CDS
CCTCTGTGGTCCATCCCCAG+AGG 0.644825 4.3:+39612057 None:intergenic
GCAACATTCTTCACCCTCTG+GGG 0.646104 4.3:-39612071 MS.gene004025:CDS
TTATGCTGGCACCCAACATG+TGG 0.647174 4.3:+39611040 None:intergenic
AAGGTTTAGAGAGAGTTGAG+AGG 0.649221 4.3:-39611247 MS.gene004025:CDS
AAGACTCGTCGTAAGAAACA+AGG 0.649427 4.3:-39612199 MS.gene004025:CDS
ATGAGGTTGAGAAATAGCAG+GGG 0.658762 4.3:+39612660 None:intergenic
GATCCTCTTGGTGTTCGGAG+AGG 0.659641 4.3:+39611961 None:intergenic
AGCTATTAGTGCTATTGACG+AGG 0.669842 4.3:-39612347 MS.gene004025:CDS
CAGAGTTATGAATATCTAAG+GGG 0.672632 4.3:+39612803 None:intergenic
CAACAGCAATAGCAGCGTCG+AGG 0.673827 4.3:-39612758 MS.gene004025:CDS
TGAATATGATAGCTTCCTTG+AGG 0.674292 4.3:-39613097 MS.gene004025:CDS
TTTGTGAAATCACTAGCTTG+AGG 0.676421 4.3:+39613210 None:intergenic
AAGTAATGAGCCAACCTCTG+TGG 0.682694 4.3:+39612043 None:intergenic
TCTTAAAATCAAGAGCAATG+AGG 0.688807 4.3:-39611570 MS.gene004025:CDS
AGCGAACAGTCCTAGAAATG+CGG 0.690521 4.3:-39611492 MS.gene004025:CDS
GAGGGATGGATCCGTCGCCG+AGG 0.691265 4.3:+39612990 None:intergenic
GTAGTGGCTGCAAAGCCAAG+TGG 0.693207 4.3:+39613036 None:intergenic
GGCCCGAGACATATAAACAG+TGG 0.698214 4.3:-39611226 MS.gene004025:CDS
CTGATGGAGAGGGAAAGTAT+CGG 0.714910 4.3:-39611299 MS.gene004025:CDS
TGGTGAAGAAGGGAGTACAG+TGG 0.749828 4.3:-39613172 MS.gene004025:CDS
GAATGGACAAGTGAAAACGG+AGG 0.763230 4.3:-39612245 MS.gene004025:CDS
CCTCTGGGGATGGACCACAG+AGG 0.797161 4.3:-39612057 MS.gene004025:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAAAAACTTTATAAGTAT+TGG + chr4.3:39612489-39612508 None:intergenic 15.0%
!! GATGAGATTTAAGAATTTAT+TGG - chr4.3:39612737-39612756 MS.gene004025:CDS 20.0%
!!! TTCTAAACTTTTCTTGAATA+GGG + chr4.3:39611719-39611738 None:intergenic 20.0%
! AATCAATATGATAGCTTTCT+TGG - chr4.3:39611199-39611218 MS.gene004025:CDS 25.0%
! AGAATGAAACAATAGATTTG+AGG - chr4.3:39612094-39612113 MS.gene004025:CDS 25.0%
! ATCAGAGTTATGAATATCTA+AGG + chr4.3:39611467-39611486 None:intergenic 25.0%
! ATTATCAGAGATGTTTGATA+AGG - chr4.3:39611945-39611964 MS.gene004025:CDS 25.0%
! TCAGAGTTATGAATATCTAA+GGG + chr4.3:39611466-39611485 None:intergenic 25.0%
! TTAGATATTCATAACTCTGA+TGG - chr4.3:39611466-39611485 MS.gene004025:CDS 25.0%
! TTCAAGAAAAGTTTAGAAGA+AGG - chr4.3:39611721-39611740 MS.gene004025:CDS 25.0%
! TTTGTTCAGTCAATTGTTAA+TGG - chr4.3:39612831-39612850 MS.gene004025:CDS 25.0%
!! AAGTATGCAAACTTTTTGAA+AGG + chr4.3:39612378-39612397 None:intergenic 25.0%
!! AAGTTTTTATCAGATAGAGA+AGG - chr4.3:39612498-39612517 MS.gene004025:CDS 25.0%
!! AGAAAGCTATCATATTGATT+TGG + chr4.3:39611199-39611218 None:intergenic 25.0%
!! CTTCTAAACTTTTCTTGAAT+AGG + chr4.3:39611720-39611739 None:intergenic 25.0%
!! GAATCATTTTCATTGCATAA+TGG + chr4.3:39611994-39612013 None:intergenic 25.0%
!! TTTTTGATGTAGACAACAAT+TGG - chr4.3:39613165-39613184 MS.gene004025:CDS 25.0%
!!! ATTGATTTTGGTATCCTATA+TGG - chr4.3:39612453-39612472 MS.gene004025:CDS 25.0%
ACCAAAATCAATGATATGGA+GGG + chr4.3:39612445-39612464 None:intergenic 30.0%
AGGAAGCTATCATATTCATT+TGG + chr4.3:39611166-39611185 None:intergenic 30.0%
ATCCAATTTGGATATGGTTA+GGG - chr4.3:39611792-39611811 MS.gene004025:CDS 30.0%
ATTGACTGAACAAAAATAGC+AGG + chr4.3:39612825-39612844 None:intergenic 30.0%
CAGAGTTATGAATATCTAAG+GGG + chr4.3:39611465-39611484 None:intergenic 30.0%
CATCAAAAACAAAGTCTTTG+TGG + chr4.3:39613154-39613173 None:intergenic 30.0%
GGATACCAAAATCAATGATA+TGG + chr4.3:39612449-39612468 None:intergenic 30.0%
TACCAAAATCAATGATATGG+AGG + chr4.3:39612446-39612465 None:intergenic 30.0%
TCCATTAATTCCGAATTCAA+TGG + chr4.3:39613101-39613120 None:intergenic 30.0%
TCCATTGAATTCGGAATTAA+TGG - chr4.3:39613097-39613116 MS.gene004025:CDS 30.0%
TCTTAAAATCAAGAGCAATG+AGG - chr4.3:39612695-39612714 MS.gene004025:CDS 30.0%
TGAATCATCCAATTTGGATA+TGG - chr4.3:39611786-39611805 MS.gene004025:CDS 30.0%
TGATTAACATGATCAGAAAC+AGG + chr4.3:39611568-39611587 None:intergenic 30.0%
TTTATATGCTTCCACATGTT+GGG - chr4.3:39613214-39613233 MS.gene004025:CDS 30.0%
! AGGAAGCTATCATATTGATT+TGG + chr4.3:39611133-39611152 None:intergenic 30.0%
! CGGAATTAATGGACAAATTT+AGG - chr4.3:39613108-39613127 MS.gene004025:CDS 30.0%
! TTTTATATGCTTCCACATGT+TGG - chr4.3:39613213-39613232 MS.gene004025:CDS 30.0%
! TTTTTATCAGATAGAGAAGG+TGG - chr4.3:39612501-39612520 MS.gene004025:CDS 30.0%
!!! TGGTTTTGAATCATCCAATT+TGG - chr4.3:39611780-39611799 MS.gene004025:CDS 30.0%
AAACAAATACAGGATGCTGA+TGG - chr4.3:39612950-39612969 MS.gene004025:CDS 35.0%
AAGAACAAGAAGAAGAAGCT+TGG - chr4.3:39611878-39611897 MS.gene004025:CDS 35.0%
AATGAGGTTGAGAAATAGCA+GGG + chr4.3:39611609-39611628 None:intergenic 35.0%
AGGCCATATAACCTTCTATA+GGG + chr4.3:39611258-39611277 None:intergenic 35.0%
ATGGAAAGAATCGAAGAAAC+TGG - chr4.3:39612579-39612598 MS.gene004025:CDS 35.0%
ATTAGCATTCCTGTTGTCAT+TGG + chr4.3:39612154-39612173 None:intergenic 35.0%
ATTCATTTGGATCAGCATCA+AGG + chr4.3:39611153-39611172 None:intergenic 35.0%
ATTCTTTGCTACACGGTTTA+GGG - chr4.3:39612869-39612888 MS.gene004025:CDS 35.0%
ATTTCTCAACCTCATTCTAC+TGG - chr4.3:39611613-39611632 MS.gene004025:CDS 35.0%
CAAACAGCTTCCATTGAATT+CGG - chr4.3:39613088-39613107 MS.gene004025:CDS 35.0%
CAAGCTAGTGATTTCACAAA+CGG - chr4.3:39611058-39611077 MS.gene004025:CDS 35.0%
CAGTCATTTGTAAGCTAAGT+GGG + chr4.3:39611384-39611403 None:intergenic 35.0%
CATATAACCTTCTATAGGGA+AGG + chr4.3:39611254-39611273 None:intergenic 35.0%
CATCCAATTTGGATATGGTT+AGG - chr4.3:39611791-39611810 MS.gene004025:CDS 35.0%
CATTAAACTCAAAGGGAACA+TGG + chr4.3:39612635-39612654 None:intergenic 35.0%
CTTCCCTATAGAAGGTTATA+TGG - chr4.3:39611252-39611271 MS.gene004025:CDS 35.0%
GAAAATGATTCCTTGCAGAA+TGG - chr4.3:39612003-39612022 MS.gene004025:CDS 35.0%
GGATTCTTCTATTACACAGA+TGG - chr4.3:39611660-39611679 MS.gene004025:CDS 35.0%
GGTATGGCATTAAACTCAAA+GGG + chr4.3:39612642-39612661 None:intergenic 35.0%
GTTCATAAACCAAATCCTGA+TGG - chr4.3:39611327-39611346 MS.gene004025:CDS 35.0%
GTTTCTTCGATTCTTTCCAT+TGG + chr4.3:39612579-39612598 None:intergenic 35.0%
TATAGAAGGTTATATGGCCT+CGG - chr4.3:39611258-39611277 MS.gene004025:CDS 35.0%
TCAGTCATTTGTAAGCTAAG+TGG + chr4.3:39611385-39611404 None:intergenic 35.0%
TGAAAAATCAGTGTCTTCGA+GGG + chr4.3:39611296-39611315 None:intergenic 35.0%
TGAATATGATAGCTTCCTTG+AGG - chr4.3:39611168-39611187 MS.gene004025:CDS 35.0%
TGGTATGGCATTAAACTCAA+AGG + chr4.3:39612643-39612662 None:intergenic 35.0%
TTTCTACCCTTCAACACAAA+CGG + chr4.3:39611829-39611848 None:intergenic 35.0%
! CATTTTCTTCCATCAGGATT+TGG + chr4.3:39611339-39611358 None:intergenic 35.0%
! GATAAGGTTTTGCTTGATGT+CGG - chr4.3:39611961-39611980 MS.gene004025:CDS 35.0%
! GATCAACATTTTCTTCCATC+AGG + chr4.3:39611345-39611364 None:intergenic 35.0%
! GTATCCTATATGGTTTTCAG+TGG - chr4.3:39612463-39612482 MS.gene004025:CDS 35.0%
! TTTGTGAAATCACTAGCTTG+AGG + chr4.3:39611058-39611077 None:intergenic 35.0%
!! ATATCTAAGGGGTGTTGATT+AGG + chr4.3:39611454-39611473 None:intergenic 35.0%
!!! ACCCTCCATATCATTGATTT+TGG - chr4.3:39612441-39612460 MS.gene004025:CDS 35.0%
!!! GACACTGATTTTTCAGATAC+TGG - chr4.3:39611301-39611320 MS.gene004025:CDS 35.0%
AAATGCAGAACCGCATTTCT+AGG + chr4.3:39612786-39612805 None:intergenic 40.0%
AAGACTCGTCGTAAGAAACA+AGG - chr4.3:39612066-39612085 MS.gene004025:CDS 40.0%
AAGCTAAGTGGGTCATAGAA+CGG + chr4.3:39611373-39611392 None:intergenic 40.0%
AAGGTTTAGAGAGAGTTGAG+AGG - chr4.3:39613018-39613037 MS.gene004025:CDS 40.0%
ACAAACGGTTACTACCACTT+TGG - chr4.3:39611073-39611092 MS.gene004025:CDS 40.0%
ACAATTGGATGCTTCAAGGT+TGG - chr4.3:39613180-39613199 MS.gene004025:CDS 40.0%
ACTACCACTTTGGTGAAGAA+GGG - chr4.3:39611083-39611102 MS.gene004025:CDS 40.0%
AGAGAATCCGTTTGTGTTGA+AGG - chr4.3:39611819-39611838 MS.gene004025:CDS 40.0%
ATGAGCTACTGAAGCAGATT+AGG - chr4.3:39612172-39612191 MS.gene004025:CDS 40.0%
ATGAGGTTGAGAAATAGCAG+GGG + chr4.3:39611608-39611627 None:intergenic 40.0%
ATTCGCCATTCTTTGCTACA+CGG - chr4.3:39612862-39612881 MS.gene004025:CDS 40.0%
ATTGGACGAAAACCAGGTAA+AGG + chr4.3:39612561-39612580 None:intergenic 40.0%
ATTGTTTCCCAATAACGCGA+TGG + chr4.3:39612663-39612682 None:intergenic 40.0%
CAATTTGGATATGGTTAGGG+AGG - chr4.3:39611795-39611814 MS.gene004025:CDS 40.0%
CATATAAACAGTGGCAGGTT+AGG - chr4.3:39613048-39613067 MS.gene004025:CDS 40.0%
CATTCTTTGCTACACGGTTT+AGG - chr4.3:39612868-39612887 MS.gene004025:CDS 40.0%
CTAAACCGTGTAGCAAAGAA+TGG + chr4.3:39612870-39612889 None:intergenic 40.0%
CTCCCTAACCATATCCAAAT+TGG + chr4.3:39611797-39611816 None:intergenic 40.0%
CTGAAAAATCAGTGTCTTCG+AGG + chr4.3:39611297-39611316 None:intergenic 40.0%
CTGCCACTGTTTATATGTCT+CGG + chr4.3:39613045-39613064 None:intergenic 40.0%
CTGTATATGCCAATGACAAC+AGG - chr4.3:39612142-39612161 MS.gene004025:CDS 40.0%
GAATGAGGTTGAGAAATAGC+AGG + chr4.3:39611610-39611629 None:intergenic 40.0%
GACAACAATTGGATGCTTCA+AGG - chr4.3:39613176-39613195 MS.gene004025:CDS 40.0%
GAGAATCCGTTTGTGTTGAA+GGG - chr4.3:39611820-39611839 MS.gene004025:CDS 40.0%
GAGGCCATATAACCTTCTAT+AGG + chr4.3:39611259-39611278 None:intergenic 40.0%
GCAGAATGGACAAGTGAAAA+CGG - chr4.3:39612017-39612036 MS.gene004025:CDS 40.0%
GTGGTAACATTGCTGTAACT+TGG + chr4.3:39613135-39613154 None:intergenic 40.0%
TACCACGCGAAAACAAATAC+AGG - chr4.3:39612940-39612959 MS.gene004025:CDS 40.0%
TACTACCACTTTGGTGAAGA+AGG - chr4.3:39611082-39611101 MS.gene004025:CDS 40.0%
TCAAAACCAGTCAAGAGTTG+AGG + chr4.3:39611769-39611788 None:intergenic 40.0%
TCACTAACACCAGTAGAATG+AGG + chr4.3:39611625-39611644 None:intergenic 40.0%
TGCCACTGTTTATATGTCTC+GGG + chr4.3:39613044-39613063 None:intergenic 40.0%
TTGGACGAAAACCAGGTAAA+GGG + chr4.3:39612560-39612579 None:intergenic 40.0%
TTGGCCACTGAAAACCATAT+AGG + chr4.3:39612470-39612489 None:intergenic 40.0%
TTGGCTCATTACTTTGCCAA+TGG - chr4.3:39612231-39612250 MS.gene004025:CDS 40.0%
TTTCACTTGTCCATTCTGCA+AGG + chr4.3:39612016-39612035 None:intergenic 40.0%
! AGCTATTAGTGCTATTGACG+AGG - chr4.3:39611918-39611937 MS.gene004025:CDS 40.0%
! ATGAACGTTGTAGCTTGTGA+AGG - chr4.3:39612999-39613018 MS.gene004025:CDS 40.0%
! CATTCTACTGGTGTTAGTGA+TGG - chr4.3:39611625-39611644 MS.gene004025:CDS 40.0%
! TCACTAGCTTGAGGAAAGTT+TGG + chr4.3:39611049-39611068 None:intergenic 40.0%
!! AAACAGGAGTATCAGGTGAA+AGG + chr4.3:39611552-39611571 None:intergenic 40.0%
!! AACAGGAGTATCAGGTGAAA+GGG + chr4.3:39611551-39611570 None:intergenic 40.0%
!! ATCCTGTATTTGTTTTCGCG+TGG + chr4.3:39612945-39612964 None:intergenic 40.0%
!! ATTGATTTGGATCAGCCTCA+AGG + chr4.3:39611186-39611205 None:intergenic 40.0%
!! TAGTGATGGACTCAGAGATT+TGG - chr4.3:39611639-39611658 MS.gene004025:CDS 40.0%
!! TGATCAGAAACAGGAGTATC+AGG + chr4.3:39611559-39611578 None:intergenic 40.0%
!! TTGGGAAACAATTCGAGTTG+AGG - chr4.3:39612671-39612690 MS.gene004025:CDS 40.0%
!! TTTACCTGGTTTTCGTCCAA+TGG - chr4.3:39612560-39612579 MS.gene004025:CDS 40.0%
AAATCAGTGTCTTCGAGGGA+TGG + chr4.3:39611292-39611311 None:intergenic 45.0%
AAGTAATGAGCCAACCTCTG+TGG + chr4.3:39612225-39612244 None:intergenic 45.0%
AATACAGGATGCTGATGGAG+AGG - chr4.3:39612955-39612974 MS.gene004025:CDS 45.0%
ACCTTCTATAGGGAAGGTAG+TGG + chr4.3:39611248-39611267 None:intergenic 45.0%
AGCGAACAGTCCTAGAAATG+CGG - chr4.3:39612773-39612792 MS.gene004025:CDS 45.0%
AGGTAAAGGGAACTCTATCC+CGG + chr4.3:39612547-39612566 None:intergenic 45.0%
AGTCACCTCCATCAAATGGA+GGG - chr4.3:39612043-39612062 MS.gene004025:CDS 45.0%
ATACAGGATGCTGATGGAGA+GGG - chr4.3:39612956-39612975 MS.gene004025:CDS 45.0%
ATGCCATACCATCGCGTTAT+TGG - chr4.3:39612652-39612671 MS.gene004025:CDS 45.0%
CGAGACATATAAACAGTGGC+AGG - chr4.3:39613043-39613062 MS.gene004025:CDS 45.0%
CTTTCCATTGGACGAAAACC+AGG + chr4.3:39612567-39612586 None:intergenic 45.0%
GAATGGACAAGTGAAAACGG+AGG - chr4.3:39612020-39612039 MS.gene004025:CDS 45.0%
TACTCCCTTCTTCACCAAAG+TGG + chr4.3:39611090-39611109 None:intergenic 45.0%
TGCCATACCATCGCGTTATT+GGG - chr4.3:39612653-39612672 MS.gene004025:CDS 45.0%
TGGATGCTTCAAGGTTGGAA+AGG - chr4.3:39613185-39613204 MS.gene004025:CDS 45.0%
TTCCCAATAACGCGATGGTA+TGG + chr4.3:39612658-39612677 None:intergenic 45.0%
! CTGATGGAGAGGGAAAGTAT+CGG - chr4.3:39612966-39612985 MS.gene004025:CDS 45.0%
! TTACTTTGCCAATGGCCTTG+AGG - chr4.3:39612239-39612258 MS.gene004025:CDS 45.0%
!! ACAGGAGTATCAGGTGAAAG+GGG + chr4.3:39611550-39611569 None:intergenic 45.0%
!! ATGGAAGCGCTTGCAATAGT+CGG + chr4.3:39612616-39612635 None:intergenic 45.0%
!! GGGTGTTGATTAGGAGAAAG+AGG + chr4.3:39611445-39611464 None:intergenic 45.0%
!! GTAGAGATCCTCTTGGTGTT+CGG + chr4.3:39612312-39612331 None:intergenic 45.0%
!! TTTCTTGGTGCTGATCCACT+TGG - chr4.3:39611214-39611233 MS.gene004025:CDS 45.0%
ACTCTATCCCGGTGATCTTC+AGG + chr4.3:39612536-39612555 None:intergenic 50.0%
AGGCAACATTCTTCACCCTC+TGG - chr4.3:39612192-39612211 MS.gene004025:CDS 50.0%
ATTCCTCTCCGAACACCAAG+AGG - chr4.3:39612301-39612320 MS.gene004025:CDS 50.0%
CCTCCAAACCTGAAGATCAC+CGG - chr4.3:39612525-39612544 MS.gene004025:CDS 50.0%
CGAGTCTTCCCTCCATTTGA+TGG + chr4.3:39612054-39612073 None:intergenic 50.0%
CTCAGCAGTAGAGATCCTCT+TGG + chr4.3:39612319-39612338 None:intergenic 50.0%
CTCCAAACCTGAAGATCACC+GGG - chr4.3:39612526-39612545 MS.gene004025:CDS 50.0%
CTTTGCTACACGGTTTAGGG+AGG - chr4.3:39612872-39612891 MS.gene004025:CDS 50.0%
GAGGAGTCACCTCCATCAAA+TGG - chr4.3:39612039-39612058 MS.gene004025:CDS 50.0%
GAGTCACCTCCATCAAATGG+AGG - chr4.3:39612042-39612061 MS.gene004025:CDS 50.0%
GCAACATTCTTCACCCTCTG+GGG - chr4.3:39612194-39612213 MS.gene004025:CDS 50.0%
GCCACTACCTTCCCTATAGA+AGG - chr4.3:39611244-39611263 MS.gene004025:CDS 50.0%
GGCAACATTCTTCACCCTCT+GGG - chr4.3:39612193-39612212 MS.gene004025:CDS 50.0%
GGCCCGAGACATATAAACAG+TGG - chr4.3:39613039-39613058 MS.gene004025:CDS 50.0%
GGGATAGAGTTCCCTTTACC+TGG - chr4.3:39612546-39612565 MS.gene004025:CDS 50.0%
GTCTTCCCTCCATTTGATGG+AGG + chr4.3:39612051-39612070 None:intergenic 50.0%
TTATGCTGGCACCCAACATG+TGG + chr4.3:39613228-39613247 None:intergenic 50.0%
! ATCCCGGTGATCTTCAGGTT+TGG + chr4.3:39612531-39612550 None:intergenic 50.0%
! ATTGCTGTTGCTGTTGCTGC+TGG + chr4.3:39611497-39611516 None:intergenic 50.0%
! TGGTGAAGAAGGGAGTACAG+TGG - chr4.3:39611093-39611112 MS.gene004025:CDS 50.0%
!! TCTTGGTGTTCGGAGAGGAA+TGG + chr4.3:39612302-39612321 None:intergenic 50.0%
AGGTTATATGGCCTCGGCGA+CGG - chr4.3:39611264-39611283 MS.gene004025:CDS 55.0%
CAACAGCAATAGCAGCGTCG+AGG - chr4.3:39611507-39611526 MS.gene004025:CDS 55.0%
CCAGTCAAGAGTTGAGGCTG+CGG + chr4.3:39611763-39611782 None:intergenic 55.0%
GTAGTGGCTGCAAAGCCAAG+TGG + chr4.3:39611232-39611251 None:intergenic 55.0%
! CATTCTTCACCCTCTGGGGA+TGG - chr4.3:39612198-39612217 MS.gene004025:CDS 55.0%
! CCGGTGATCTTCAGGTTTGG+AGG + chr4.3:39612528-39612547 None:intergenic 55.0%
!! GATCCTCTTGGTGTTCGGAG+AGG + chr4.3:39612307-39612326 None:intergenic 55.0%
AAGGCGTGCCTCAAGGCCAT+TGG + chr4.3:39612250-39612269 None:intergenic 60.0%
CCGCAGCCTCAACTCTTGAC+TGG - chr4.3:39611760-39611779 MS.gene004025:CDS 60.0%
CTCCAGTACCATCGCCGACA+AGG + chr4.3:39612269-39612288 None:intergenic 60.0%
CTCTGTGGTCCATCCCCAGA+GGG + chr4.3:39612210-39612229 None:intergenic 60.0%
GTCAAGAGTTGAGGCTGCGG+AGG + chr4.3:39611760-39611779 None:intergenic 60.0%
TGGGGATGGACCACAGAGGT+TGG - chr4.3:39612212-39612231 MS.gene004025:CDS 60.0%
CCTCTGTGGTCCATCCCCAG+AGG + chr4.3:39612211-39612230 None:intergenic 65.0%
CGCCTTGTCGGCGATGGTAC+TGG - chr4.3:39612264-39612283 MS.gene004025:CDS 65.0%
GGCCTTGAGGCACGCCTTGT+CGG - chr4.3:39612252-39612271 MS.gene004025:CDS 65.0%
! CCTCTGGGGATGGACCACAG+AGG - chr4.3:39612208-39612227 MS.gene004025:CDS 65.0%
CGCCGACAAGGCGTGCCTCA+AGG + chr4.3:39612257-39612276 None:intergenic 70.0%
GAGGCACGCCTTGTCGGCGA+TGG - chr4.3:39612258-39612277 MS.gene004025:CDS 70.0%
GAGGGATGGATCCGTCGCCG+AGG + chr4.3:39611278-39611297 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 39611040 39613247 39611040 ID=MS.gene004025
chr4.3 mRNA 39611040 39613247 39611040 ID=MS.gene004025.t1;Parent=MS.gene004025
chr4.3 exon 39611040 39613247 39611040 ID=MS.gene004025.t1.exon1;Parent=MS.gene004025.t1
chr4.3 CDS 39611040 39613247 39611040 ID=cds.MS.gene004025.t1;Parent=MS.gene004025.t1
Gene Sequence

>MS.gene004025

ATGGATCCAAACTTTCCTCAAGCTAGTGATTTCACAAACGGTTACTACCACTTTGGTGAAGAAGGGAGTACAGTGGTCTCTGAGTTTGATCCAAATCAATATGATAGCTTCCTTGATGCTGATCCAAATGAATATGATAGCTTCCTTGAGGCTGATCCAAATCAATATGATAGCTTTCTTGGTGCTGATCCACTTGGCTTTGCAGCCACTACCTTCCCTATAGAAGGTTATATGGCCTCGGCGACGGATCCATCCCTCGAAGACACTGATTTTTCAGATACTGGTAAGTTCATAAACCAAATCCTGATGGAAGAAAATGTTGATCAGAGACCGTTCTATGACCCACTTAGCTTACAAATGACTGAAAAATCATTCTATGATGCTCTTTTACACACAAATAAACCTCTTTCTCCTAATCAACACCCCTTAGATATTCATAACTCTGATGGCACAACCAGCAGCAACAGCAACAGCAATAGCAGCGTCGAGGATTCTCGAGAGTTAAAACCCCTTTCACCTGATACTCCTGTTTCTGATCATGTTAATCATGTTTTTCAGTTTAATTCCCCTGCTATTTCTCAACCTCATTCTACTGGTGTTAGTGATGGACTCAGAGATTTGGATTCTTCTATTACACAGATGGCACAAAACATTTTCAGTGATGTAGATTCTGTTTCCCTATTCAAGAAAAGTTTAGAAGAAGGCAACAAGTTTCTTCCTCCGCAGCCTCAACTCTTGACTGGTTTTGAATCATCCAATTTGGATATGGTTAGGGAGGAAGAGAATCCGTTTGTGTTGAAGGGTAGAAAGAATCACAAGCGCGAAGAAAGTTTTAGTAAAGAACAAGAAGAAGAAGCTTGGAGAAGTAGCAAGCAATCAGCTATTAGTGCTATTGACGAGGATGAATTATCAGAGATGTTTGATAAGGTTTTGCTTGATGTCGGACATTTGCCATTATGCAATGAAAATGATTCCTTGCAGAATGGACAAGTGAAAACGGAGGAGTCACCTCCATCAAATGGAGGGAAGACTCGTCGTAAGAAACAAGGCAAGAAGAATGAAACAATAGATTTGAGGAATCTTCTGCTTATGTGTTCACAATCTGTATATGCCAATGACAACAGGAATGCTAATGAGCTACTGAAGCAGATTAGGCAACATTCTTCACCCTCTGGGGATGGACCACAGAGGTTGGCTCATTACTTTGCCAATGGCCTTGAGGCACGCCTTGTCGGCGATGGTACTGGAGCACAAACATTCCATTCCTCTCCGAACACCAAGAGGATCTCTACTGCTGAGTTTTTGAAAGCATATCAAGTTCATCTATCTACTAGTCCTTTCAAAAAGTTTGCATACTTCTTTGCAAATAAAATGATTATGAAAGCTTCTGCAAATGCTGAAACCCTCCATATCATTGATTTTGGTATCCTATATGGTTTTCAGTGGCCAATACTTATAAAGTTTTTATCAGATAGAGAAGGTGGACCTCCAAACCTGAAGATCACCGGGATAGAGTTCCCTTTACCTGGTTTTCGTCCAATGGAAAGAATCGAAGAAACTGGTCGTCGTCTTGCCGACTATTGCAAGCGCTTCCATGTTCCCTTTGAGTTTAATGCCATACCATCGCGTTATTGGGAAACAATTCGAGTTGAGGATCTTAAAATCAAGAGCAATGAGGTTGTTGTTGTGAACTCTTTGATGAGATTTAAGAATTTATTGGATGAAAGTATTGAAGCGAACAGTCCTAGAAATGCGGTTCTGCATTTAATCAGAAAGATAAATCCTGCTATTTTTGTTCAGTCAATTGTTAATGGATCATATAATTCGCCATTCTTTGCTACACGGTTTAGGGAGGCGCTGTTTCATTTTTCTGCTCTTTATGATATGTTTGACACTGTGATACCACGCGAAAACAAATACAGGATGCTGATGGAGAGGGAAAGTATCGGTAGAGAAGCTATGAACGTTGTAGCTTGTGAAGGTTTAGAGAGAGTTGAGAGGCCCGAGACATATAAACAGTGGCAGGTTAGGAATACAAGAGCTGATTTCAAACAGCTTCCATTGAATTCGGAATTAATGGACAAATTTAGGACCAAGTTACAGCAATGTTACCACAAAGACTTTGTTTTTGATGTAGACAACAATTGGATGCTTCAAGGTTGGAAAGGACGCATTTTATATGCTTCCACATGTTGGGTGCCAGCATAA
Protein sequence

>MS.gene004025.t1

MDPNFPQASDFTNGYYHFGEEGSTVVSEFDPNQYDSFLDADPNEYDSFLEADPNQYDSFLGADPLGFAATTFPIEGYMASATDPSLEDTDFSDTGKFINQILMEENVDQRPFYDPLSLQMTEKSFYDALLHTNKPLSPNQHPLDIHNSDGTTSSNSNSNSSVEDSRELKPLSPDTPVSDHVNHVFQFNSPAISQPHSTGVSDGLRDLDSSITQMAQNIFSDVDSVSLFKKSLEEGNKFLPPQPQLLTGFESSNLDMVREEENPFVLKGRKNHKREESFSKEQEEEAWRSSKQSAISAIDEDELSEMFDKVLLDVGHLPLCNENDSLQNGQVKTEESPPSNGGKTRRKKQGKKNETIDLRNLLLMCSQSVYANDNRNANELLKQIRQHSSPSGDGPQRLAHYFANGLEARLVGDGTGAQTFHSSPNTKRISTAEFLKAYQVHLSTSPFKKFAYFFANKMIMKASANAETLHIIDFGILYGFQWPILIKFLSDREGGPPNLKITGIEFPLPGFRPMERIEETGRRLADYCKRFHVPFEFNAIPSRYWETIRVEDLKIKSNEVVVVNSLMRFKNLLDESIEANSPRNAVLHLIRKINPAIFVQSIVNGSYNSPFFATRFREALFHFSALYDMFDTVIPRENKYRMLMERESIGREAMNVVACEGLERVERPETYKQWQVRNTRADFKQLPLNSELMDKFRTKLQQCYHKDFVFDVDNNWMLQGWKGRILYASTCWVPA