AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004026

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004026.t1 MTR_4g064160 95.633 687 25 4 1 683 1 686 0.0 1358
MS.gene004026.t1 MTR_7g062120 48.759 685 322 10 23 683 63 742 0.0 632
MS.gene004026.t1 MTR_2g097410 48.753 722 311 18 8 683 35 743 0.0 626
MS.gene004026.t1 MTR_4g064150 48.755 683 296 14 25 683 83 735 0.0 606
MS.gene004026.t1 MTR_4g064120 48.343 664 279 12 25 683 24 628 0.0 597
MS.gene004026.t1 MTR_4g064180 47.741 664 283 11 25 683 24 628 0.0 592
MS.gene004026.t1 MTR_2g097310 56.566 495 180 7 192 683 178 640 0.0 557
MS.gene004026.t1 MTR_2g097390 55.686 510 191 8 181 683 162 643 0.0 557
MS.gene004026.t1 MTR_2g097350 60.586 444 162 6 243 683 209 642 0.0 553
MS.gene004026.t1 MTR_2g097467 44.428 673 296 16 28 683 45 656 0.0 534
MS.gene004026.t1 MTR_2g097473 44.889 675 290 16 28 683 45 656 0.0 532
MS.gene004026.t1 MTR_2g097463 44.294 666 298 14 33 683 50 657 0.0 530
MS.gene004026.t1 MTR_4g064200 55.702 456 188 5 238 683 201 652 1.22e-171 506
MS.gene004026.t1 MTR_2g097380 49.804 510 179 10 180 683 125 563 7.56e-160 472
MS.gene004026.t1 MTR_2g082090 32.368 380 254 2 301 680 202 578 9.27e-68 233
MS.gene004026.t1 MTR_5g097480 32.796 372 249 1 309 680 173 543 1.15e-65 226
MS.gene004026.t1 MTR_1g029420 32.973 370 247 1 311 680 223 591 7.48e-64 223
MS.gene004026.t1 MTR_7g027190 33.250 400 237 5 298 680 286 672 9.58e-64 224
MS.gene004026.t1 MTR_3g056110 30.979 439 287 8 248 680 113 541 3.99e-62 216
MS.gene004026.t1 MTR_2g026250 31.283 374 255 2 308 680 223 595 4.46e-62 218
MS.gene004026.t1 MTR_4g133660 31.200 375 256 2 308 681 170 543 1.18e-61 215
MS.gene004026.t1 MTR_3g089055 31.351 370 253 1 311 680 196 564 7.61e-61 214
MS.gene004026.t1 MTR_3g089055 31.351 370 253 1 311 680 201 569 8.59e-61 214
MS.gene004026.t1 MTR_5g094450 32.361 377 248 5 309 680 157 531 3.00e-60 211
MS.gene004026.t1 MTR_8g442410 30.000 420 266 9 276 680 118 524 2.50e-54 195
MS.gene004026.t1 MTR_4g104020 29.688 384 247 6 310 680 146 519 3.21e-54 194
MS.gene004026.t1 MTR_4g076140 28.571 427 240 6 313 680 48 468 1.25e-48 177
MS.gene004026.t1 MTR_3g065980 30.155 388 241 7 308 680 167 539 2.33e-47 176
MS.gene004026.t1 MTR_7g074650 30.328 366 241 5 317 682 442 793 2.06e-46 177
MS.gene004026.t1 MTR_3g022830 27.512 418 244 7 314 680 26 435 8.30e-43 160
MS.gene004026.t1 MTR_4g097080 26.913 379 265 5 311 682 131 504 2.82e-39 152
MS.gene004026.t1 MTR_4g102790 26.494 385 263 9 309 682 288 663 7.28e-39 153
MS.gene004026.t1 MTR_2g089100 27.340 406 254 9 310 683 49 445 1.50e-38 149
MS.gene004026.t1 MTR_2g034250 26.873 387 262 7 308 680 205 584 1.77e-36 145
MS.gene004026.t1 MTR_2g034260 25.641 390 262 6 308 680 205 583 2.16e-36 145
MS.gene004026.t1 MTR_4g076020 28.226 372 234 8 317 683 88 431 5.02e-36 141
MS.gene004026.t1 MTR_7g057230 27.807 374 251 7 314 680 295 656 2.48e-34 139
MS.gene004026.t1 MTR_4g077760 28.760 379 248 9 310 680 190 554 4.58e-34 137
MS.gene004026.t1 MTR_1g069725 28.609 381 244 13 308 681 104 463 1.38e-33 135
MS.gene004026.t1 MTR_2g034280 25.255 392 259 6 308 680 198 574 2.61e-32 132
MS.gene004026.t1 MTR_5g009080 33.810 210 132 2 313 521 56 259 1.43e-31 129
MS.gene004026.t1 MTR_5g009080 30.201 149 104 0 532 680 329 477 6.99e-20 94.0
MS.gene004026.t1 MTR_7g109580 26.772 381 260 8 308 680 184 553 1.50e-31 130
MS.gene004026.t1 MTR_5g015490 26.893 383 262 7 313 682 114 491 2.03e-31 129
MS.gene004026.t1 MTR_1g096030 28.244 393 243 15 313 680 80 458 7.33e-29 120
MS.gene004026.t1 MTR_6g047750 26.963 382 254 9 311 680 293 661 1.99e-27 118
MS.gene004026.t1 MTR_1g086970 25.253 396 254 15 308 680 101 477 2.14e-26 113
MS.gene004026.t1 MTR_3g072710 25.648 386 245 12 322 681 131 500 4.04e-25 110
MS.gene004026.t1 MTR_4g095500 24.882 422 262 12 310 683 56 470 2.58e-24 107
MS.gene004026.t1 MTR_5g015950 24.466 421 267 12 310 683 32 448 5.07e-24 106
MS.gene004026.t1 MTR_3g053270 24.466 421 267 12 310 683 32 448 5.07e-24 106
MS.gene004026.t1 MTR_5g058860 27.027 370 237 12 332 681 141 497 2.59e-23 104
MS.gene004026.t1 MTR_2g099110 24.422 389 265 8 313 682 74 452 6.46e-22 99.8
MS.gene004026.t1 MTR_8g093070 25.570 395 241 18 310 683 136 498 2.20e-17 86.3
MS.gene004026.t1 MTR_0092s0100 22.439 410 278 10 283 680 346 727 5.12e-17 85.9
MS.gene004026.t1 MTR_0092s0100 22.439 410 278 10 283 680 349 730 5.36e-17 85.5
MS.gene004026.t1 MTR_8g020840 23.810 483 295 17 233 680 103 547 4.46e-13 72.8
MS.gene004026.t1 MTR_4g026485 19.417 412 284 11 283 680 246 623 7.16e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004026.t1 AT1G07530 54.532 684 272 10 37 683 86 767 0.0 719
MS.gene004026.t1 AT2G29060 50.071 703 285 17 9 682 28 693 0.0 652
MS.gene004026.t1 AT2G29060 50.073 687 272 16 9 682 28 656 0.0 645
MS.gene004026.t1 AT1G07520 47.067 699 306 19 8 683 39 696 0.0 549
MS.gene004026.t1 AT1G07520 47.067 699 306 19 8 683 38 695 0.0 548
MS.gene004026.t1 AT2G37650 44.265 680 319 13 27 682 59 702 0.0 538
MS.gene004026.t1 AT2G37650 44.265 680 319 13 27 682 71 714 0.0 538
MS.gene004026.t1 AT2G29065 46.061 660 283 16 34 683 34 630 0.0 535
MS.gene004026.t1 AT1G07520 51.526 557 219 16 149 683 4 531 2.09e-177 516
MS.gene004026.t1 AT1G07520 51.526 557 219 16 149 683 4 531 2.09e-177 516
MS.gene004026.t1 AT5G59450 47.009 468 242 4 220 683 135 600 1.27e-152 456
MS.gene004026.t1 AT3G46600 47.875 447 221 4 237 683 115 549 1.72e-144 432
MS.gene004026.t1 AT3G46600 47.875 447 221 4 237 683 147 581 2.04e-144 433
MS.gene004026.t1 AT3G46600 51.604 374 180 1 310 683 1 373 6.82e-139 412
MS.gene004026.t1 AT1G50600 33.871 372 245 1 309 680 155 525 1.16e-66 228
MS.gene004026.t1 AT1G50600 33.871 372 245 1 309 680 230 600 6.05e-66 228
MS.gene004026.t1 AT1G50600 33.871 372 245 1 309 680 226 596 7.50e-66 228
MS.gene004026.t1 AT5G48150 33.512 373 246 2 309 680 118 489 2.35e-64 221
MS.gene004026.t1 AT5G48150 33.512 373 246 2 309 680 118 489 2.35e-64 221
MS.gene004026.t1 AT5G48150 33.512 373 246 2 309 680 118 489 2.35e-64 221
MS.gene004026.t1 AT5G48150 33.512 373 246 2 309 680 118 489 2.35e-64 221
MS.gene004026.t1 AT1G21450 33.514 370 246 0 311 680 223 592 8.15e-64 223
MS.gene004026.t1 AT2G04890 32.796 372 242 2 309 680 49 412 1.43e-63 217
MS.gene004026.t1 AT4G17230 32.533 375 252 1 308 682 153 526 4.68e-55 197
MS.gene004026.t1 AT4G17230 32.533 375 252 1 308 682 153 526 4.68e-55 197
MS.gene004026.t1 AT3G03450 31.398 379 242 9 308 680 178 544 8.41e-51 186
MS.gene004026.t1 AT3G54220 30.000 370 240 6 317 682 297 651 3.81e-49 183
MS.gene004026.t1 AT1G66350 32.181 376 232 10 308 680 150 505 6.19e-49 179
MS.gene004026.t1 AT1G14920 30.688 378 241 8 308 680 167 528 7.88e-47 174
MS.gene004026.t1 AT5G17490 31.347 386 227 11 308 680 155 515 2.43e-46 172
MS.gene004026.t1 AT5G66770 31.436 369 239 8 317 680 224 583 4.98e-45 170
MS.gene004026.t1 AT2G01570 30.343 379 241 10 308 680 219 580 2.43e-43 165
MS.gene004026.t1 AT3G50650 28.158 380 255 7 309 680 172 541 3.16e-37 147
MS.gene004026.t1 AT5G41920 26.873 387 258 7 301 680 31 399 3.80e-36 141
MS.gene004026.t1 AT4G37650 27.110 391 260 8 312 682 145 530 1.69e-35 141
MS.gene004026.t1 AT1G63100 29.830 352 232 8 309 655 248 589 2.15e-35 142
MS.gene004026.t1 AT1G63100 29.830 352 232 8 309 655 273 614 2.15e-35 142
MS.gene004026.t1 AT5G52510 27.224 371 236 11 329 680 284 639 1.10e-31 131
MS.gene004026.t1 AT3G49950 27.720 386 257 11 313 682 30 409 2.64e-30 124
MS.gene004026.t1 AT1G50420 30.189 212 139 2 313 521 57 262 4.66e-29 121
MS.gene004026.t1 AT1G50420 32.215 149 101 0 532 680 330 478 5.68e-21 97.1
MS.gene004026.t1 AT1G55580 24.941 421 249 11 310 680 41 444 5.91e-29 120
MS.gene004026.t1 AT4G08250 25.055 451 304 14 251 680 39 476 1.61e-25 111
MS.gene004026.t1 AT3G13840 22.772 404 265 14 297 680 133 509 4.65e-13 72.8
MS.gene004026.t1 AT4G00150 21.149 383 264 7 306 680 201 553 5.37e-12 69.3

Find 168 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCACACCTTTGGATTTA+TGG 0.102821 4.3:-39617368 MS.gene004026:CDS
ACTCTCCACATTGTAGATTT+TGG 0.171656 4.3:-39616122 MS.gene004026:CDS
GTGTAACCGGTGGTGGATTT+AGG 0.183770 4.3:-39616937 MS.gene004026:CDS
AAACAAAATCAACAGGAAAA+TGG 0.197416 4.3:+39617011 None:intergenic
TCTTCTTCTGTAGCATTGTT+TGG 0.273251 4.3:+39617322 None:intergenic
TTACATTAACCAAATGCTTA+TGG 0.276145 4.3:-39617254 MS.gene004026:CDS
CTTCAAGTCCTCAACTTTGA+TGG 0.287285 4.3:+39615887 None:intergenic
AGTAGTATGTATAAAGATAT+TGG 0.295160 4.3:+39615743 None:intergenic
AATGGAAGGTTCGGAATATA+AGG 0.302414 4.3:-39615509 MS.gene004026:CDS
TTACTAAGCAAGTTTGAATC+AGG 0.311461 4.3:+39616905 None:intergenic
GTCGGCAGCAGAGTTTCTTT+TGG 0.312934 4.3:+39616247 None:intergenic
GGCAGGCCTGAAGCCAGGTT+GGG 0.342579 4.3:+39616004 None:intergenic
AAAGAAGAAGCAAATAATTC+TGG 0.356533 4.3:-39616518 MS.gene004026:CDS
ACAACCAATGGATTCACTTT+AGG 0.359550 4.3:+39616827 None:intergenic
AGAAGGGTGTTGTGGATCTA+AGG 0.362488 4.3:-39616460 MS.gene004026:CDS
TGTAACCGGTGGTGGATTTA+GGG 0.363942 4.3:-39616936 MS.gene004026:CDS
TCAATGCTATTGCACAGAAA+TGG 0.371102 4.3:-39615914 MS.gene004026:CDS
TGATGTTTGAGAAAGAGTTC+TGG 0.377337 4.3:-39615602 MS.gene004026:CDS
AAATGGGTTTGCAATATAGA+AGG 0.381555 4.3:+39617028 None:intergenic
TCATAGATGGATCACACCTT+TGG 0.385301 4.3:-39617376 None:intergenic
GATAAGGTGCTGCTTGGAAC+GGG 0.386499 4.3:-39616590 MS.gene004026:CDS
CGGCAGGCCTGAAGCCAGGT+TGG 0.388141 4.3:+39616003 None:intergenic
AGTAATAGCAGTAGCGATTT+CGG 0.389012 4.3:-39616959 MS.gene004026:CDS
ATGTATGTTTCATGATTCAT+TGG 0.389528 4.3:-39617209 MS.gene004026:CDS
TGTCTTCTTCCATAAGCATT+TGG 0.390726 4.3:+39617245 None:intergenic
AACAAAATCAACAGGAAAAT+GGG 0.391391 4.3:+39617012 None:intergenic
TTCCATTTCCTGTATGTCTC+TGG 0.394619 4.3:+39615525 None:intergenic
AAATCTTTCTATGAAGTCAT+TGG 0.396607 4.3:-39617169 MS.gene004026:CDS
GCCGAGAGAGTCCAGGAGAC+AGG 0.397232 4.3:-39615984 MS.gene004026:CDS
CTTCTAAAAGAAGGAACAAA+AGG 0.397285 4.3:+39616788 None:intergenic
GGCCAGAGACATACAGGAAA+TGG 0.403238 4.3:-39615527 MS.gene004026:CDS
TCGCTAGATTCAAAATTGTA+TGG 0.404227 4.3:+39616156 None:intergenic
TCTGTAGCATTGTTTGGAGC+TGG 0.404262 4.3:+39617328 None:intergenic
TTACTGTTAAAACTATCATC+AGG 0.409982 4.3:+39617118 None:intergenic
TTTGGAGCTGGTTGAAGAAA+AGG 0.410352 4.3:+39617340 None:intergenic
TTGTGGTGAATTCTGTTTGT+CGG 0.410916 4.3:-39615845 MS.gene004026:CDS
GACAGGCTTCAATATACATT+TGG 0.411821 4.3:+39616210 None:intergenic
GCAGGCCTGAAGCCAGGTTG+GGG 0.414806 4.3:+39616005 None:intergenic
AAGGGAGGTTCAGGAGGTAG+TGG 0.418003 4.3:-39616768 MS.gene004026:CDS
TTTATACATACTACTGTCAA+TGG 0.418864 4.3:-39615735 MS.gene004026:CDS
CAATGCTATTGCACAGAAAT+GGG 0.422432 4.3:-39615913 MS.gene004026:CDS
AAGGCAATATTCTTCTCCAT+TGG 0.423667 4.3:-39616363 MS.gene004026:CDS
GCAATAGCATTGAACTCAAA+TGG 0.428641 4.3:+39615924 None:intergenic
GTCTCCTGGACTCTCTCGGC+AGG 0.430954 4.3:+39615987 None:intergenic
TTGAACCGTGTCGCAAAGAA+TGG 0.433149 4.3:+39615699 None:intergenic
GTAGTATGTATAAAGATATT+GGG 0.433213 4.3:+39615744 None:intergenic
AGGAGGAAGCGTAAATTATT+CGG 0.433733 4.3:+39615358 None:intergenic
GCATCCGTTACGGCTTTCAA+TGG 0.436217 4.3:-39616100 MS.gene004026:CDS
CCTCCTAAGCTGCTCTTGAC+AGG 0.440626 4.3:-39616038 MS.gene004026:CDS
AATTTACGCTTCCTCCTGTT+GGG 0.441011 4.3:-39615352 MS.gene004026:CDS
CTAGGCGAAGCCCTGTCTCC+TGG 0.442369 4.3:+39615973 None:intergenic
TAATTTCAAAACAGCATCCC+TGG 0.447641 4.3:+39615782 None:intergenic
TGTTGCCTTGTTTCTTACCT+CGG 0.450630 4.3:+39616483 None:intergenic
TGCAAGCTTCTTGAATGGAC+AGG 0.451062 4.3:+39616193 None:intergenic
TTGGAGCTGGTTGAAGAAAA+GGG 0.451298 4.3:+39617341 None:intergenic
CTCTCGGCAGGCCTGAAGCC+AGG 0.453760 4.3:+39615999 None:intergenic
GATGTTTGAGAAAGAGTTCT+GGG 0.455142 4.3:-39615601 MS.gene004026:CDS
AGGCAATATTCTTCTCCATT+GGG 0.457992 4.3:-39616362 MS.gene004026:CDS
TCGAGAGGCCAGAGACATAC+AGG 0.459712 4.3:-39615533 MS.gene004026:CDS
ATCCCTGTCAAGAGCAGCTT+AGG 0.460560 4.3:+39616035 None:intergenic
GTCCTATGCAGGTACCCAAC+AGG 0.461795 4.3:+39615338 None:intergenic
AGAAAGGAAGAGAAGGCTAA+TGG 0.462372 4.3:-39616563 MS.gene004026:CDS
ATTCTTTGCGACACGGTTCA+AGG 0.463902 4.3:-39615697 MS.gene004026:CDS
AGTAGCGATTTCGGTGTAAC+CGG 0.469350 4.3:-39616950 MS.gene004026:CDS
TTTCAAGCTAGTTCAATGAA+TGG 0.469664 4.3:-39616989 MS.gene004026:CDS
AGAAGAAGAAGATTCACCTT+TGG 0.473696 4.3:-39617308 MS.gene004026:CDS
CATACAGGAAATGGAAGGTT+CGG 0.475232 4.3:-39615518 MS.gene004026:CDS
TGAACCGTGTCGCAAAGAAT+GGG 0.479940 4.3:+39615700 None:intergenic
ATGAATGCAAGCTTCTTGAA+TGG 0.481139 4.3:+39616188 None:intergenic
TAATTTACGCTTCCTCCTGT+TGG 0.482014 4.3:-39615353 MS.gene004026:CDS
TTCAGGAGGTAGTGGTTGAG+AGG 0.482313 4.3:-39616760 MS.gene004026:CDS
TCAGGGCTTTGTACAGAAGA+AGG 0.485059 4.3:+39617136 None:intergenic
GCTCTAGAAGCACGCATGGC+TGG 0.493848 4.3:-39616302 MS.gene004026:CDS
GAAGCACGCATGGCTGGTAC+TGG 0.498249 4.3:-39616296 MS.gene004026:CDS
TGGATTTAAGCAACTCCCCT+TGG 0.498423 4.3:-39615484 MS.gene004026:CDS
GATTTCGGTGTAACCGGTGG+TGG 0.506809 4.3:-39616944 MS.gene004026:CDS
TCAGGAGGTAGTGGTTGAGA+GGG 0.507729 4.3:-39616759 MS.gene004026:CDS
CAGGCCTGCCGAGAGAGTCC+AGG 0.510078 4.3:-39615991 MS.gene004026:CDS
GATAAACGATAAATAAGGGC+AGG 0.512065 4.3:+39616077 None:intergenic
ATGAGTTATTGAGGCAGATA+AGG 0.512815 4.3:-39616382 MS.gene004026:CDS
CTCTTCAAATCAACAACCAA+TGG 0.512943 4.3:+39616815 None:intergenic
TTATACATACTACTGTCAAT+GGG 0.515737 4.3:-39615734 MS.gene004026:CDS
CTGCATGCTGCAAGGATGGA+AGG 0.517952 4.3:-39615382 MS.gene004026:CDS
CTCCTGTTGGGTACCTGCAT+AGG 0.518592 4.3:-39615340 None:intergenic
GCTATCGGAGCTGTTTGATA+AGG 0.519129 4.3:-39616606 MS.gene004026:CDS
GGGGAGGAAGAATCACGAGC+GGG 0.521175 4.3:-39616699 MS.gene004026:CDS
GAAAGGAAGAGAAGGCTAAT+GGG 0.521362 4.3:-39616562 MS.gene004026:CDS
AAGAAACTCTGCTGCCGACA+TGG 0.522015 4.3:-39616243 MS.gene004026:CDS
AACGTTCACAATACCTTGCT+AGG 0.524003 4.3:+39615955 None:intergenic
CCGAGAGAGTCCAGGAGACA+GGG 0.524887 4.3:-39615983 MS.gene004026:CDS
CAGGCCTGAAGCCAGGTTGG+GGG 0.525521 4.3:+39616006 None:intergenic
ATCATTGCCAAGTCCTATGC+AGG 0.526668 4.3:+39615327 None:intergenic
TGATGATGACAGTGAGCTAT+CGG 0.527539 4.3:-39616621 MS.gene004026:CDS
TGAACCTCCCTTCTAAAAGA+AGG 0.528231 4.3:+39616779 None:intergenic
TATTCTTCTCCATTGGGTGA+TGG 0.529010 4.3:-39616356 MS.gene004026:CDS
ATCGTTTATCAAAACGACCT+GGG 0.530234 4.3:-39616064 MS.gene004026:CDS
GAAGCAAATAATTCTGGTGG+TGG 0.534103 4.3:-39616512 MS.gene004026:CDS
TGATAAGGTGCTGCTTGGAA+CGG 0.538662 4.3:-39616591 MS.gene004026:CDS
GAAACTGCATGCTGCAAGGA+TGG 0.539727 4.3:-39615386 MS.gene004026:CDS
CTGTTTGATAAGGTGCTGCT+TGG 0.542358 4.3:-39616596 MS.gene004026:CDS
GAGAAATCAATTTCATCCAA+AGG 0.543170 4.3:+39617292 None:intergenic
GCTTGGAACGGGGTGCAGAA+AGG 0.543390 4.3:-39616579 MS.gene004026:CDS
TCTGCCTAAAGTGAATCCAT+TGG 0.544272 4.3:-39616831 MS.gene004026:CDS
CCCTGTCTCCTGGACTCTCT+CGG 0.545286 4.3:+39615983 None:intergenic
TTAATGAGATGTTTATCCAA+GGG 0.545301 4.3:+39615468 None:intergenic
GTTAATGAGATGTTTATCCA+AGG 0.545722 4.3:+39615467 None:intergenic
CAGTAGTAGTAATTCTGTTG+AGG 0.547986 4.3:-39617092 MS.gene004026:CDS
CCTGTCAAGAGCAGCTTAGG+AGG 0.549308 4.3:+39616038 None:intergenic
TACTGTTAAAACTATCATCA+GGG 0.554998 4.3:+39617119 None:intergenic
CAGTTGTGTTGAATAGTCCC+AGG 0.555999 4.3:-39615800 MS.gene004026:CDS
ACCGGTGGTGGATTTAGGGA+TGG 0.556953 4.3:-39616932 MS.gene004026:CDS
GGGTGATGGATCTCAGAGGC+TGG 0.557398 4.3:-39616342 MS.gene004026:CDS
AAGGTTCGGAATATAAGGGC+TGG 0.559644 4.3:-39615504 MS.gene004026:CDS
GCAATTTGAGAGGGGTGTGG+AGG 0.561220 4.3:-39616867 MS.gene004026:CDS
ATGGAAGGTTCGGAATATAA+GGG 0.563303 4.3:-39615508 MS.gene004026:CDS
TTCTAAAAGAAGGAACAAAA+GGG 0.563412 4.3:+39616789 None:intergenic
ATGAATATTATAGCTTGTGA+AGG 0.563558 4.3:-39615567 MS.gene004026:CDS
TATCGTTTATCAAAACGACC+TGG 0.572065 4.3:-39616065 MS.gene004026:CDS
CTCCTAAGCTGCTCTTGACA+GGG 0.575690 4.3:-39616037 MS.gene004026:CDS
AGGCAACAAGAAGGGTGTTG+TGG 0.575933 4.3:-39616468 MS.gene004026:CDS
TGCATGCTGCAAGGATGGAA+GGG 0.578283 4.3:-39615381 MS.gene004026:CDS
AATTTCAAAACAGCATCCCT+GGG 0.578395 4.3:+39615783 None:intergenic
TCGTTTATCAAAACGACCTG+GGG 0.584413 4.3:-39616063 MS.gene004026:CDS
ATGCCCCATTCTTTGCGACA+CGG 0.584723 4.3:-39615704 MS.gene004026:CDS
GGGAGGAAGAATCACGAGCG+GGG 0.584989 4.3:-39616698 MS.gene004026:CDS
AGTCTGTATTGCAATTTGAG+AGG 0.585618 4.3:-39616877 MS.gene004026:CDS
AGACAGGGCTTCGCCTAGCA+AGG 0.589816 4.3:-39615968 MS.gene004026:CDS
TGTTTGAGAAAGAGTTCTGG+GGG 0.594182 4.3:-39615599 MS.gene004026:CDS
ATGGGAAACCATCAAAGTTG+AGG 0.594978 4.3:-39615895 MS.gene004026:CDS
AAGAAACAAGGCAACAAGAA+GGG 0.596770 4.3:-39616476 MS.gene004026:CDS
ATTGCAATTTGAGAGGGGTG+TGG 0.597668 4.3:-39616870 MS.gene004026:CDS
TTGGTACGCTTTCACCATGT+CGG 0.598816 4.3:+39616229 None:intergenic
TCCATCCCTAAATCCACCAC+CGG 0.599728 4.3:+39616931 None:intergenic
CAAATGCTAATGAGTTATTG+AGG 0.600348 4.3:-39616391 MS.gene004026:CDS
GTCTGTATTGCAATTTGAGA+GGG 0.601880 4.3:-39616876 MS.gene004026:CDS
CAGGGCTTTGTACAGAAGAA+GGG 0.601972 4.3:+39617137 None:intergenic
CAGGCCATTGAAAGCCGTAA+CGG 0.602915 4.3:+39616096 None:intergenic
TAAGAAACAAGGCAACAAGA+AGG 0.603179 4.3:-39616477 MS.gene004026:CDS
AGCTTGTGAAGGTTCTCAGA+GGG 0.603648 4.3:-39615556 MS.gene004026:CDS
AATGCTTATGGAAGAAGACA+TGG 0.605099 4.3:-39617242 MS.gene004026:CDS
AGGGTTATCCATAAATCCAA+AGG 0.611812 4.3:+39617360 None:intergenic
GAAGAAGCAAATAATTCTGG+TGG 0.623065 4.3:-39616515 MS.gene004026:CDS
TAATGAGATGTTTATCCAAG+GGG 0.623159 4.3:+39615469 None:intergenic
GTTAATGTAACGAAGAACAG+TGG 0.624527 4.3:+39617267 None:intergenic
AGCGATTTCGGTGTAACCGG+TGG 0.625322 4.3:-39616947 MS.gene004026:CDS
GAATGCTCTAGAAGCACGCA+TGG 0.632297 4.3:-39616306 MS.gene004026:CDS
AATGAATCATGAAACATACA+TGG 0.637630 4.3:+39617211 None:intergenic
AAGAGCCGAGGTAAGAAACA+AGG 0.639801 4.3:-39616488 MS.gene004026:CDS
GGAAGAGAAGGCTAATGGGT+CGG 0.641207 4.3:-39616558 MS.gene004026:CDS
ATGTTTGAGAAAGAGTTCTG+GGG 0.644129 4.3:-39615600 MS.gene004026:CDS
GAACCGTGTCGCAAAGAATG+GGG 0.648096 4.3:+39615701 None:intergenic
GGGGTGCAGAAAGGAAGAGA+AGG 0.651471 4.3:-39616570 MS.gene004026:CDS
GATGGAAACTGCATGCTGCA+AGG 0.652853 4.3:-39615390 MS.gene004026:CDS
CTTTGCGACACGGTTCAAGG+AGG 0.657665 4.3:-39615694 MS.gene004026:CDS
AAGGTTCTCAGAGGGTCGAG+AGG 0.658012 4.3:-39615548 MS.gene004026:CDS
TGTGGATCTAAGGACAATGT+TGG 0.660423 4.3:-39616450 MS.gene004026:CDS
AGTTGTGTTGAATAGTCCCA+GGG 0.661922 4.3:-39615799 MS.gene004026:CDS
CTCTGAGATCCATCACCCAA+TGG 0.662757 4.3:+39616347 None:intergenic
AGAGACATACAGGAAATGGA+AGG 0.664271 4.3:-39615523 MS.gene004026:CDS
CATTGGGTGATGGATCTCAG+AGG 0.665660 4.3:-39616346 MS.gene004026:CDS
ATAAGGTGCTGCTTGGAACG+GGG 0.670615 4.3:-39616589 MS.gene004026:CDS
TCTGGTGGTGGAAAGAGCCG+AGG 0.679483 4.3:-39616500 MS.gene004026:CDS
GGATGCCAAAATCTACAATG+TGG 0.681308 4.3:+39616117 None:intergenic
CTATGCAGGTACCCAACAGG+AGG 0.684912 4.3:+39615341 None:intergenic
TAGCTTGTGAAGGTTCTCAG+AGG 0.697926 4.3:-39615557 MS.gene004026:CDS
CGTTTATCAAAACGACCTGG+GGG 0.713845 4.3:-39616062 MS.gene004026:CDS
TCTGTATTGCAATTTGAGAG+GGG 0.714329 4.3:-39616875 MS.gene004026:CDS
TTGCTCGTGAAGATCAAACG+AGG 0.754863 4.3:-39615626 MS.gene004026:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGATAAACGATAAATAA+GGG + chr4.3:39616641-39616660 None:intergenic 15.0%
!! AGTAGTATGTATAAAGATAT+TGG + chr4.3:39616971-39616990 None:intergenic 20.0%
!! GTAGTATGTATAAAGATATT+GGG + chr4.3:39616970-39616989 None:intergenic 20.0%
!!! ATGCTGTTTTGAAATTAATT+AGG - chr4.3:39616935-39616954 MS.gene004026:CDS 20.0%
!!! GTTTTGATAAACGATAAATA+AGG + chr4.3:39616642-39616661 None:intergenic 20.0%
!!! TGCTGTTTTGAAATTAATTA+GGG - chr4.3:39616936-39616955 MS.gene004026:CDS 20.0%
! AAACAAAATCAACAGGAAAA+TGG + chr4.3:39615703-39615722 None:intergenic 25.0%
! AAAGAAAAATGAACTTCTTG+TGG - chr4.3:39616849-39616868 MS.gene004026:CDS 25.0%
! AAAGAAGAAGCAAATAATTC+TGG - chr4.3:39616193-39616212 MS.gene004026:CDS 25.0%
! AAATCTTTCTATGAAGTCAT+TGG - chr4.3:39615542-39615561 MS.gene004026:CDS 25.0%
! AACAAAATCAACAGGAAAAT+GGG + chr4.3:39615702-39615721 None:intergenic 25.0%
! AATGAATCATGAAACATACA+TGG + chr4.3:39615503-39615522 None:intergenic 25.0%
! ATGTATGTTTCATGATTCAT+TGG - chr4.3:39615502-39615521 MS.gene004026:CDS 25.0%
! TACTGTTAAAACTATCATCA+GGG + chr4.3:39615595-39615614 None:intergenic 25.0%
! TTAATGAGATGTTTATCCAA+GGG + chr4.3:39617246-39617265 None:intergenic 25.0%
! TTACATTAACCAAATGCTTA+TGG - chr4.3:39615457-39615476 MS.gene004026:CDS 25.0%
! TTACTGTTAAAACTATCATC+AGG + chr4.3:39615596-39615615 None:intergenic 25.0%
! TTATACATACTACTGTCAAT+GGG - chr4.3:39616977-39616996 MS.gene004026:CDS 25.0%
! TTTATACATACTACTGTCAA+TGG - chr4.3:39616976-39616995 MS.gene004026:CDS 25.0%
!! ATGAATATTATAGCTTGTGA+AGG - chr4.3:39617144-39617163 MS.gene004026:CDS 25.0%
!! TTCTAAAAGAAGGAACAAAA+GGG + chr4.3:39615925-39615944 None:intergenic 25.0%
!!! TTTTGTTCCTTCTTTTAGAA+GGG - chr4.3:39615925-39615944 MS.gene004026:CDS 25.0%
CAAATGCTAATGAGTTATTG+AGG - chr4.3:39616320-39616339 MS.gene004026:CDS 30.0%
GAGAAATCAATTTCATCCAA+AGG + chr4.3:39615422-39615441 None:intergenic 30.0%
GCTTGAAAAACAAAATCAAC+AGG + chr4.3:39615710-39615729 None:intergenic 30.0%
GTTAATGAGATGTTTATCCA+AGG + chr4.3:39617247-39617266 None:intergenic 30.0%
TAATGAGATGTTTATCCAAG+GGG + chr4.3:39617245-39617264 None:intergenic 30.0%
TCGCTAGATTCAAAATTGTA+TGG + chr4.3:39616558-39616577 None:intergenic 30.0%
TTACTAAGCAAGTTTGAATC+AGG + chr4.3:39615809-39615828 None:intergenic 30.0%
TTTCAAGCTAGTTCAATGAA+TGG - chr4.3:39615722-39615741 MS.gene004026:CDS 30.0%
! AAATGGGTTTGCAATATAGA+AGG + chr4.3:39615686-39615705 None:intergenic 30.0%
! CTTCTAAAAGAAGGAACAAA+AGG + chr4.3:39615926-39615945 None:intergenic 30.0%
! CTTTTGTTCCTTCTTTTAGA+AGG - chr4.3:39615924-39615943 MS.gene004026:CDS 30.0%
! TACAATTTTGAATCTAGCGA+AGG - chr4.3:39616558-39616577 MS.gene004026:CDS 30.0%
!! GATTTTATGCTTGTTGAAGA+TGG - chr4.3:39617303-39617322 MS.gene004026:CDS 30.0%
AAGAAACAAGGCAACAAGAA+GGG - chr4.3:39616235-39616254 MS.gene004026:CDS 35.0%
AAGGCAATATTCTTCTCCAT+TGG - chr4.3:39616348-39616367 MS.gene004026:CDS 35.0%
AATGCTTATGGAAGAAGACA+TGG - chr4.3:39615469-39615488 MS.gene004026:CDS 35.0%
AATGGAAGGTTCGGAATATA+AGG - chr4.3:39617202-39617221 MS.gene004026:CDS 35.0%
AATTTCAAAACAGCATCCCT+GGG + chr4.3:39616931-39616950 None:intergenic 35.0%
AGAAGAAGAAGATTCACCTT+TGG - chr4.3:39615403-39615422 MS.gene004026:CDS 35.0%
AGGCAATATTCTTCTCCATT+GGG - chr4.3:39616349-39616368 MS.gene004026:CDS 35.0%
AGGGTTATCCATAAATCCAA+AGG + chr4.3:39615354-39615373 None:intergenic 35.0%
AGTAATAGCAGTAGCGATTT+CGG - chr4.3:39615752-39615771 MS.gene004026:CDS 35.0%
AGTCTGTATTGCAATTTGAG+AGG - chr4.3:39615834-39615853 MS.gene004026:CDS 35.0%
ATCGTTTATCAAAACGACCT+GGG - chr4.3:39616647-39616666 MS.gene004026:CDS 35.0%
ATGAATGCAAGCTTCTTGAA+TGG + chr4.3:39616526-39616545 None:intergenic 35.0%
ATGAGTTATTGAGGCAGATA+AGG - chr4.3:39616329-39616348 MS.gene004026:CDS 35.0%
ATGGAAGGTTCGGAATATAA+GGG - chr4.3:39617203-39617222 MS.gene004026:CDS 35.0%
ATGTTTGAGAAAGAGTTCTG+GGG - chr4.3:39617111-39617130 MS.gene004026:CDS 35.0%
CAATGCTATTGCACAGAAAT+GGG - chr4.3:39616798-39616817 MS.gene004026:CDS 35.0%
CAGTAGTAGTAATTCTGTTG+AGG - chr4.3:39615619-39615638 MS.gene004026:CDS 35.0%
CTCTTCAAATCAACAACCAA+TGG + chr4.3:39615899-39615918 None:intergenic 35.0%
GAAGAAGCAAATAATTCTGG+TGG - chr4.3:39616196-39616215 MS.gene004026:CDS 35.0%
GACAGGCTTCAATATACATT+TGG + chr4.3:39616504-39616523 None:intergenic 35.0%
GATAAACGATAAATAAGGGC+AGG + chr4.3:39616637-39616656 None:intergenic 35.0%
GATGTTTGAGAAAGAGTTCT+GGG - chr4.3:39617110-39617129 MS.gene004026:CDS 35.0%
GTCTGTATTGCAATTTGAGA+GGG - chr4.3:39615835-39615854 MS.gene004026:CDS 35.0%
GTTAATGTAACGAAGAACAG+TGG + chr4.3:39615447-39615466 None:intergenic 35.0%
TAAGAAACAAGGCAACAAGA+AGG - chr4.3:39616234-39616253 MS.gene004026:CDS 35.0%
TAATTTCAAAACAGCATCCC+TGG + chr4.3:39616932-39616951 None:intergenic 35.0%
TATCGTTTATCAAAACGACC+TGG - chr4.3:39616646-39616665 MS.gene004026:CDS 35.0%
TCAATGCTATTGCACAGAAA+TGG - chr4.3:39616797-39616816 MS.gene004026:CDS 35.0%
TCTGTATTGCAATTTGAGAG+GGG - chr4.3:39615836-39615855 MS.gene004026:CDS 35.0%
TCTTCTTCTGTAGCATTGTT+TGG + chr4.3:39615392-39615411 None:intergenic 35.0%
TGATGTTTGAGAAAGAGTTC+TGG - chr4.3:39617109-39617128 MS.gene004026:CDS 35.0%
TGTCTTCTTCCATAAGCATT+TGG + chr4.3:39615469-39615488 None:intergenic 35.0%
TTGTGGTGAATTCTGTTTGT+CGG - chr4.3:39616866-39616885 MS.gene004026:CDS 35.0%
! ACAACCAATGGATTCACTTT+AGG + chr4.3:39615887-39615906 None:intergenic 35.0%
! ACTCTCCACATTGTAGATTT+TGG - chr4.3:39616589-39616608 MS.gene004026:CDS 35.0%
! AGGAGGAAGCGTAAATTATT+CGG + chr4.3:39617356-39617375 None:intergenic 35.0%
! GCAATAGCATTGAACTCAAA+TGG + chr4.3:39616790-39616809 None:intergenic 35.0%
AACGTTCACAATACCTTGCT+AGG + chr4.3:39616759-39616778 None:intergenic 40.0%
AATTTACGCTTCCTCCTGTT+GGG - chr4.3:39617359-39617378 MS.gene004026:CDS 40.0%
AGAAAGGAAGAGAAGGCTAA+TGG - chr4.3:39616148-39616167 MS.gene004026:CDS 40.0%
AGAGACATACAGGAAATGGA+AGG - chr4.3:39617188-39617207 MS.gene004026:CDS 40.0%
AGTTGTGTTGAATAGTCCCA+GGG - chr4.3:39616912-39616931 MS.gene004026:CDS 40.0%
ATGGGAAACCATCAAAGTTG+AGG - chr4.3:39616816-39616835 MS.gene004026:CDS 40.0%
CATACAGGAAATGGAAGGTT+CGG - chr4.3:39617193-39617212 MS.gene004026:CDS 40.0%
CTTCAAGTCCTCAACTTTGA+TGG + chr4.3:39616827-39616846 None:intergenic 40.0%
GAAAGGAAGAGAAGGCTAAT+GGG - chr4.3:39616149-39616168 MS.gene004026:CDS 40.0%
GAAGCAAATAATTCTGGTGG+TGG - chr4.3:39616199-39616218 MS.gene004026:CDS 40.0%
GGATCACACCTTTGGATTTA+TGG - chr4.3:39615343-39615362 MS.gene004026:CDS 40.0%
GGATGCCAAAATCTACAATG+TGG + chr4.3:39616597-39616616 None:intergenic 40.0%
TAATTTACGCTTCCTCCTGT+TGG - chr4.3:39617358-39617377 MS.gene004026:CDS 40.0%
TATTCTTCTCCATTGGGTGA+TGG - chr4.3:39616355-39616374 MS.gene004026:CDS 40.0%
TCGTTTATCAAAACGACCTG+GGG - chr4.3:39616648-39616667 MS.gene004026:CDS 40.0%
TCTGCCTAAAGTGAATCCAT+TGG - chr4.3:39615880-39615899 MS.gene004026:CDS 40.0%
TGTGGATCTAAGGACAATGT+TGG - chr4.3:39616261-39616280 MS.gene004026:CDS 40.0%
TTCCATTTCCTGTATGTCTC+TGG + chr4.3:39617189-39617208 None:intergenic 40.0%
TTGGAGCTGGTTGAAGAAAA+GGG + chr4.3:39615373-39615392 None:intergenic 40.0%
! TGAACCTCCCTTCTAAAAGA+AGG + chr4.3:39615935-39615954 None:intergenic 40.0%
! TGATGATGACAGTGAGCTAT+CGG - chr4.3:39616090-39616109 MS.gene004026:CDS 40.0%
! TGTTTGAGAAAGAGTTCTGG+GGG - chr4.3:39617112-39617131 MS.gene004026:CDS 40.0%
! TTTGGAGCTGGTTGAAGAAA+AGG + chr4.3:39615374-39615393 None:intergenic 40.0%
!! GTAGATTTTGGCATCCGTTA+CGG - chr4.3:39616601-39616620 MS.gene004026:CDS 40.0%
!! TGTTCCTTCTTTTAGAAGGG+AGG - chr4.3:39615928-39615947 MS.gene004026:CDS 40.0%
!! TGTTGCCTTGTTTCTTACCT+CGG + chr4.3:39616231-39616250 None:intergenic 40.0%
!! TTCTTTTAGAAGGGAGGTTC+AGG - chr4.3:39615934-39615953 MS.gene004026:CDS 40.0%
AAGAGCCGAGGTAAGAAACA+AGG - chr4.3:39616223-39616242 MS.gene004026:CDS 45.0%
AAGGTTCGGAATATAAGGGC+TGG - chr4.3:39617207-39617226 MS.gene004026:CDS 45.0%
AGCTTGTGAAGGTTCTCAGA+GGG - chr4.3:39617155-39617174 MS.gene004026:CDS 45.0%
ATTCTTTGCGACACGGTTCA+AGG - chr4.3:39617014-39617033 MS.gene004026:CDS 45.0%
ATTGCAATTTGAGAGGGGTG+TGG - chr4.3:39615841-39615860 MS.gene004026:CDS 45.0%
CAGTTGTGTTGAATAGTCCC+AGG - chr4.3:39616911-39616930 MS.gene004026:CDS 45.0%
CGTTTATCAAAACGACCTGG+GGG - chr4.3:39616649-39616668 MS.gene004026:CDS 45.0%
GCTATCGGAGCTGTTTGATA+AGG - chr4.3:39616105-39616124 MS.gene004026:CDS 45.0%
TGAACCGTGTCGCAAAGAAT+GGG + chr4.3:39617014-39617033 None:intergenic 45.0%
TGCAAGCTTCTTGAATGGAC+AGG + chr4.3:39616521-39616540 None:intergenic 45.0%
TGGATTTAAGCAACTCCCCT+TGG - chr4.3:39617227-39617246 MS.gene004026:CDS 45.0%
TTGAACCGTGTCGCAAAGAA+TGG + chr4.3:39617015-39617034 None:intergenic 45.0%
TTGCTCGTGAAGATCAAACG+AGG - chr4.3:39617085-39617104 MS.gene004026:CDS 45.0%
TTGGTACGCTTTCACCATGT+CGG + chr4.3:39616485-39616504 None:intergenic 45.0%
! AGTAGCGATTTCGGTGTAAC+CGG - chr4.3:39615761-39615780 MS.gene004026:CDS 45.0%
! CAGGGCTTTGTACAGAAGAA+GGG + chr4.3:39615577-39615596 None:intergenic 45.0%
! TAGCTTGTGAAGGTTCTCAG+AGG - chr4.3:39617154-39617173 MS.gene004026:CDS 45.0%
! TCAGGGCTTTGTACAGAAGA+AGG + chr4.3:39615578-39615597 None:intergenic 45.0%
! TCTGTAGCATTGTTTGGAGC+TGG + chr4.3:39615386-39615405 None:intergenic 45.0%
!! AACGTTTTTCAGCTGAGTCG+AGG - chr4.3:39615990-39616009 MS.gene004026:CDS 45.0%
!! ACGTTTTTCAGCTGAGTCGA+GGG - chr4.3:39615991-39616010 MS.gene004026:CDS 45.0%
!! AGAAGGGTGTTGTGGATCTA+AGG - chr4.3:39616251-39616270 MS.gene004026:CDS 45.0%
!! CTGTTTGATAAGGTGCTGCT+TGG - chr4.3:39616115-39616134 MS.gene004026:CDS 45.0%
!! TGATAAGGTGCTGCTTGGAA+CGG - chr4.3:39616120-39616139 MS.gene004026:CDS 45.0%
!! TGTAACCGGTGGTGGATTTA+GGG - chr4.3:39615775-39615794 MS.gene004026:CDS 45.0%
!! TTTTAGAAGGGAGGTTCAGG+AGG - chr4.3:39615937-39615956 MS.gene004026:CDS 45.0%
AAGAAACTCTGCTGCCGACA+TGG - chr4.3:39616468-39616487 MS.gene004026:CDS 50.0%
ATCCCTGTCAAGAGCAGCTT+AGG + chr4.3:39616679-39616698 None:intergenic 50.0%
ATGCCCCATTCTTTGCGACA+CGG - chr4.3:39617007-39617026 MS.gene004026:CDS 50.0%
CAGGCCATTGAAAGCCGTAA+CGG + chr4.3:39616618-39616637 None:intergenic 50.0%
CTCCTAAGCTGCTCTTGACA+GGG - chr4.3:39616674-39616693 MS.gene004026:CDS 50.0%
GAAACTGCATGCTGCAAGGA+TGG - chr4.3:39617325-39617344 MS.gene004026:CDS 50.0%
GAACCGTGTCGCAAAGAATG+GGG + chr4.3:39617013-39617032 None:intergenic 50.0%
GATGGAAACTGCATGCTGCA+AGG - chr4.3:39617321-39617340 MS.gene004026:CDS 50.0%
GCATCCGTTACGGCTTTCAA+TGG - chr4.3:39616611-39616630 MS.gene004026:CDS 50.0%
GGCCAGAGACATACAGGAAA+TGG - chr4.3:39617184-39617203 MS.gene004026:CDS 50.0%
TCCATCCCTAAATCCACCAC+CGG + chr4.3:39615783-39615802 None:intergenic 50.0%
TGCATGCTGCAAGGATGGAA+GGG - chr4.3:39617330-39617349 MS.gene004026:CDS 50.0%
! GGAAGAGAAGGCTAATGGGT+CGG - chr4.3:39616153-39616172 MS.gene004026:CDS 50.0%
! GTCGGCAGCAGAGTTTCTTT+TGG + chr4.3:39616467-39616486 None:intergenic 50.0%
! TCAGGAGGTAGTGGTTGAGA+GGG - chr4.3:39615952-39615971 MS.gene004026:CDS 50.0%
! TTCAGGAGGTAGTGGTTGAG+AGG - chr4.3:39615951-39615970 MS.gene004026:CDS 50.0%
!! AGGCAACAAGAAGGGTGTTG+TGG - chr4.3:39616243-39616262 MS.gene004026:CDS 50.0%
!! ATAAGGTGCTGCTTGGAACG+GGG - chr4.3:39616122-39616141 MS.gene004026:CDS 50.0%
!! CATTGGGTGATGGATCTCAG+AGG - chr4.3:39616365-39616384 MS.gene004026:CDS 50.0%
!! CGTTTTTCAGCTGAGTCGAG+GGG - chr4.3:39615992-39616011 MS.gene004026:CDS 50.0%
!! CTCTGAGATCCATCACCCAA+TGG + chr4.3:39616367-39616386 None:intergenic 50.0%
!! GAATGCTCTAGAAGCACGCA+TGG - chr4.3:39616405-39616424 MS.gene004026:CDS 50.0%
!! GATAAGGTGCTGCTTGGAAC+GGG - chr4.3:39616121-39616140 MS.gene004026:CDS 50.0%
!! GTGTAACCGGTGGTGGATTT+AGG - chr4.3:39615774-39615793 MS.gene004026:CDS 50.0%
!! GTTTTTCAGCTGAGTCGAGG+GGG - chr4.3:39615993-39616012 MS.gene004026:CDS 50.0%
!! TGAGAAAGAGTTCTGGGGGA+GGG - chr4.3:39617116-39617135 MS.gene004026:CDS 50.0%
!! TTGAGAAAGAGTTCTGGGGG+AGG - chr4.3:39617115-39617134 MS.gene004026:CDS 50.0%
AAGGGAGGTTCAGGAGGTAG+TGG - chr4.3:39615943-39615962 MS.gene004026:CDS 55.0%
AAGGTTCTCAGAGGGTCGAG+AGG - chr4.3:39617163-39617182 MS.gene004026:CDS 55.0%
CCTCCTAAGCTGCTCTTGAC+AGG - chr4.3:39616673-39616692 MS.gene004026:CDS 55.0%
CCTGTCAAGAGCAGCTTAGG+AGG + chr4.3:39616676-39616695 None:intergenic 55.0%
CTATGCAGGTACCCAACAGG+AGG + chr4.3:39617373-39617392 None:intergenic 55.0%
CTGCATGCTGCAAGGATGGA+AGG - chr4.3:39617329-39617348 MS.gene004026:CDS 55.0%
CTTTGCGACACGGTTCAAGG+AGG - chr4.3:39617017-39617036 MS.gene004026:CDS 55.0%
GGGGTGCAGAAAGGAAGAGA+AGG - chr4.3:39616141-39616160 MS.gene004026:CDS 55.0%
TCGAGAGGCCAGAGACATAC+AGG - chr4.3:39617178-39617197 MS.gene004026:CDS 55.0%
! AGCGATTTCGGTGTAACCGG+TGG - chr4.3:39615764-39615783 MS.gene004026:CDS 55.0%
! GATTTCGGTGTAACCGGTGG+TGG - chr4.3:39615767-39615786 MS.gene004026:CDS 55.0%
! GCAATTTGAGAGGGGTGTGG+AGG - chr4.3:39615844-39615863 MS.gene004026:CDS 55.0%
!! ACCGGTGGTGGATTTAGGGA+TGG - chr4.3:39615779-39615798 MS.gene004026:CDS 55.0%
AACTCCCCCAACCTGGCTTC+AGG - chr4.3:39616701-39616720 MS.gene004026:CDS 60.0%
AGACAGGGCTTCGCCTAGCA+AGG - chr4.3:39616743-39616762 MS.gene004026:CDS 60.0%
CCCTGTCTCCTGGACTCTCT+CGG + chr4.3:39616731-39616750 None:intergenic 60.0%
CCGAGAGAGTCCAGGAGACA+GGG - chr4.3:39616728-39616747 MS.gene004026:CDS 60.0%
GAAGCACGCATGGCTGGTAC+TGG - chr4.3:39616415-39616434 MS.gene004026:CDS 60.0%
GCTTGGAACGGGGTGCAGAA+AGG - chr4.3:39616132-39616151 MS.gene004026:CDS 60.0%
GGGAGGAAGAATCACGAGCG+GGG - chr4.3:39616013-39616032 MS.gene004026:CDS 60.0%
GGGATAGAACTCCCCCAACC+TGG - chr4.3:39616694-39616713 MS.gene004026:CDS 60.0%
GGGGAGGAAGAATCACGAGC+GGG - chr4.3:39616012-39616031 MS.gene004026:CDS 60.0%
GGGGGAGGAAGAATCACGAG+CGG - chr4.3:39616011-39616030 MS.gene004026:CDS 60.0%
TTTCAGCTGAGTCGAGGGGG+AGG - chr4.3:39615996-39616015 MS.gene004026:CDS 60.0%
!! GCTCTAGAAGCACGCATGGC+TGG - chr4.3:39616409-39616428 MS.gene004026:CDS 60.0%
!! GGGTGATGGATCTCAGAGGC+TGG - chr4.3:39616369-39616388 MS.gene004026:CDS 60.0%
!! TCTGGTGGTGGAAAGAGCCG+AGG - chr4.3:39616211-39616230 MS.gene004026:CDS 60.0%
AGCAGCTTAGGAGGTCCCCC+AGG + chr4.3:39616667-39616686 None:intergenic 65.0%
CTAGGCGAAGCCCTGTCTCC+TGG + chr4.3:39616741-39616760 None:intergenic 65.0%
GCAGGCCTGAAGCCAGGTTG+GGG + chr4.3:39616709-39616728 None:intergenic 65.0%
GCCGAGAGAGTCCAGGAGAC+AGG - chr4.3:39616727-39616746 MS.gene004026:CDS 65.0%
GGCAGGCCTGAAGCCAGGTT+GGG + chr4.3:39616710-39616729 None:intergenic 65.0%
GTCTCCTGGACTCTCTCGGC+AGG + chr4.3:39616727-39616746 None:intergenic 65.0%
! CAGGCCTGAAGCCAGGTTGG+GGG + chr4.3:39616708-39616727 None:intergenic 65.0%
CAGGCCTGCCGAGAGAGTCC+AGG - chr4.3:39616720-39616739 MS.gene004026:CDS 70.0%
CGGCAGGCCTGAAGCCAGGT+TGG + chr4.3:39616711-39616730 None:intergenic 70.0%
CTCTCGGCAGGCCTGAAGCC+AGG + chr4.3:39616715-39616734 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 39615341 39617392 39615341 ID=MS.gene004026
chr4.3 mRNA 39615341 39617392 39615341 ID=MS.gene004026.t1;Parent=MS.gene004026
chr4.3 exon 39615341 39617392 39615341 ID=MS.gene004026.t1.exon1;Parent=MS.gene004026.t1
chr4.3 CDS 39615341 39617392 39615341 ID=cds.MS.gene004026.t1;Parent=MS.gene004026.t1
Gene Sequence

>MS.gene004026

ATGGATCACACCTTTGGATTTATGGATAACCCTTTTCTTCAACCAGCTCCAAACAATGCTACAGAAGAAGAAGATTCACCTTTGGATGAAATTGATTTCTCTTCCACTGTTCTTCGTTACATTAACCAAATGCTTATGGAAGAAGACATGGATATGAAACCATGTATGTTTCATGATTCATTGGCTTTACAAGCTGCTGAAAAATCTTTCTATGAAGTCATTGGTGAGACTTACCCTTCTTCTGTACAAAGCCCTGATGATAGTTTTAACAGTAATTTCAGTAGTAGTAATTCTGTTGAGGTTGAATCATATTTGAGTAATTTTGATGTTTCTGAGCATAATCCTTCTATATTGCAAACCCATTTTCCTGTTGATTTTGTTTTTCAAGCTAGTTCAATGAATGGTAGTAGTAGTAATAGCAGTAGCGATTTCGGTGTAACCGGTGGTGGATTTAGGGATGGATTTCCTGATTCAAACTTGCTTAGTAAGAATGAGTCTGTATTGCAATTTGAGAGGGGTGTGGAGGAAGCTAATAAGTTTCTGCCTAAAGTGAATCCATTGGTTGTTGATTTGAAGAGTAACCCTTTTGTTCCTTCTTTTAGAAGGGAGGTTCAGGAGGTAGTGGTTGAGAGGGAGAGCAATGAAAGAGAACGTTTTTCAGCTGAGTCGAGGGGGAGGAAGAATCACGAGCGGGGAGATGAATTGAGTTTACAAGATGAGAGAAGTAACAAACAATCAGCTGTTTATACTGATGATGACAGTGAGCTATCGGAGCTGTTTGATAAGGTGCTGCTTGGAACGGGGTGCAGAAAGGAAGAGAAGGCTAATGGGTCGGATACGAGCGTGCAGCAGAAAGAAGAAGCAAATAATTCTGGTGGTGGAAAGAGCCGAGGTAAGAAACAAGGCAACAAGAAGGGTGTTGTGGATCTAAGGACAATGTTGGTTCTATGTGCACACTATGTTTCTTCTGATGATCGTGCAAATGCTAATGAGTTATTGAGGCAGATAAGGCAATATTCTTCTCCATTGGGTGATGGATCTCAGAGGCTGGCACATTGCTTTGCGAATGCTCTAGAAGCACGCATGGCTGGTACTGGCACTCAGATTTATACTGCTTTATACTCCAAAAGAAACTCTGCTGCCGACATGGTGAAAGCGTACCAAATGTATATTGAAGCCTGTCCATTCAAGAAGCTTGCATTCATTTTCGCGAACCATACAATTTTGAATCTAGCGAAGGAAGTAGAAACTCTCCACATTGTAGATTTTGGCATCCGTTACGGCTTTCAATGGCCTGCCCTTATTTATCGTTTATCAAAACGACCTGGGGGACCTCCTAAGCTGCTCTTGACAGGGATAGAACTCCCCCAACCTGGCTTCAGGCCTGCCGAGAGAGTCCAGGAGACAGGGCTTCGCCTAGCAAGGTATTGTGAACGTTTTAATGTTCCATTTGAGTTCAATGCTATTGCACAGAAATGGGAAACCATCAAAGTTGAGGACTTGAAGATAAAGAAAAATGAACTTCTTGTGGTGAATTCTGTTTGTCGGCTTAAAAATTTACTTGACGAGACAGTTGTGTTGAATAGTCCCAGGGATGCTGTTTTGAAATTAATTAGGGACGCAAATCCCAATATCTTTATACATACTACTGTCAATGGGAGCTACAATGCCCCATTCTTTGCGACACGGTTCAAGGAGGCTCTTTTCAATTACTCTACAATGTTTGATGTGCTTGACATCAATGTTGCTCGTGAAGATCAAACGAGGTTGATGTTTGAGAAAGAGTTCTGGGGGAGGGAAGTAATGAATATTATAGCTTGTGAAGGTTCTCAGAGGGTCGAGAGGCCAGAGACATACAGGAAATGGAAGGTTCGGAATATAAGGGCTGGATTTAAGCAACTCCCCTTGGATAAACATCTCATTAACAAGTTAAGATGCAAGTTGAAAGATGTGTATCATAGTGATTTTATGCTTGTTGAAGATGGAAACTGCATGCTGCAAGGATGGAAGGGCCGAATAATTTACGCTTCCTCCTGTTGGGTACCTGCATAG
Protein sequence

>MS.gene004026.t1

MDHTFGFMDNPFLQPAPNNATEEEDSPLDEIDFSSTVLRYINQMLMEEDMDMKPCMFHDSLALQAAEKSFYEVIGETYPSSVQSPDDSFNSNFSSSNSVEVESYLSNFDVSEHNPSILQTHFPVDFVFQASSMNGSSSNSSSDFGVTGGGFRDGFPDSNLLSKNESVLQFERGVEEANKFLPKVNPLVVDLKSNPFVPSFRREVQEVVVERESNERERFSAESRGRKNHERGDELSLQDERSNKQSAVYTDDDSELSELFDKVLLGTGCRKEEKANGSDTSVQQKEEANNSGGGKSRGKKQGNKKGVVDLRTMLVLCAHYVSSDDRANANELLRQIRQYSSPLGDGSQRLAHCFANALEARMAGTGTQIYTALYSKRNSAADMVKAYQMYIEACPFKKLAFIFANHTILNLAKEVETLHIVDFGIRYGFQWPALIYRLSKRPGGPPKLLLTGIELPQPGFRPAERVQETGLRLARYCERFNVPFEFNAIAQKWETIKVEDLKIKKNELLVVNSVCRLKNLLDETVVLNSPRDAVLKLIRDANPNIFIHTTVNGSYNAPFFATRFKEALFNYSTMFDVLDINVAREDQTRLMFEKEFWGREVMNIIACEGSQRVERPETYRKWKVRNIRAGFKQLPLDKHLINKLRCKLKDVYHSDFMLVEDGNCMLQGWKGRIIYASSCWVPA