AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004150.t1 MTR_4g461080 95.062 162 8 0 13 174 1 162 4.52e-103 298
MS.gene004150.t1 MTR_4g461080 100.000 41 0 0 167 207 237 277 8.34e-23 93.2
MS.gene004150.t1 MTR_2g093220 68.553 159 38 6 19 168 18 173 1.72e-60 191
MS.gene004150.t1 MTR_3g077750 52.027 148 47 5 2 141 28 159 1.66e-35 127
MS.gene004150.t1 MTR_3g091820 65.556 90 29 1 8 97 12 99 1.95e-34 124
MS.gene004150.t1 MTR_1g077600 56.701 97 38 1 3 95 1 97 3.42e-34 122
MS.gene004150.t1 MTR_1g077600 56.701 97 38 1 3 95 1 97 3.80e-34 123
MS.gene004150.t1 MTR_4g063780 66.304 92 26 2 25 111 58 149 7.45e-34 123
MS.gene004150.t1 MTR_8g015840 70.270 74 22 0 29 102 8 81 1.57e-32 117
MS.gene004150.t1 MTR_2g096740 60.000 85 34 0 12 96 33 117 2.94e-32 118
MS.gene004150.t1 MTR_3g090430 64.706 85 30 0 15 99 25 109 3.31e-32 119
MS.gene004150.t1 MTR_2g096740 60.000 85 34 0 12 96 48 132 4.09e-32 118
MS.gene004150.t1 MTR_4g022370 66.250 80 26 1 17 95 57 136 4.58e-32 119
MS.gene004150.t1 MTR_4g088580 59.596 99 30 2 42 130 56 154 9.79e-32 119
MS.gene004150.t1 MTR_8g027295 66.234 77 24 1 23 99 8 82 1.10e-31 116
MS.gene004150.t1 MTR_1g056810 64.368 87 27 2 43 125 14 100 2.86e-31 115
MS.gene004150.t1 MTR_7g059400 68.831 77 23 1 20 95 54 130 3.75e-31 116
MS.gene004150.t1 MTR_2g014060 85.965 57 6 1 36 92 22 76 8.17e-31 115
MS.gene004150.t1 MTR_8g479350 52.294 109 37 3 39 147 66 159 8.48e-31 115
MS.gene004150.t1 MTR_8g068210 58.333 84 35 0 30 113 21 104 1.03e-30 115
MS.gene004150.t1 MTR_7g024670 72.857 70 18 1 40 108 76 145 1.30e-29 113
MS.gene004150.t1 MTR_4g109980 86.792 53 7 0 39 91 38 90 1.47e-29 112
MS.gene004150.t1 MTR_8g079060 70.588 68 18 1 32 97 18 85 1.91e-29 109
MS.gene004150.t1 MTR_5g031440 80.702 57 10 1 35 91 38 93 2.06e-29 112
MS.gene004150.t1 MTR_2g014170 90.000 50 5 0 40 89 44 93 2.42e-28 108
MS.gene004150.t1 MTR_2g013370 60.465 86 31 2 10 95 17 99 7.32e-28 106
MS.gene004150.t1 MTR_2g059540 88.000 50 6 0 40 89 30 79 1.27e-27 103
MS.gene004150.t1 MTR_4g089095 80.702 57 10 1 39 95 48 103 3.06e-27 105
MS.gene004150.t1 MTR_4g089095 80.702 57 10 1 39 95 14 69 3.24e-27 104
MS.gene004150.t1 MTR_3g435480 66.197 71 23 1 29 99 125 194 2.53e-26 105
MS.gene004150.t1 MTR_7g010950 67.143 70 22 1 29 98 124 192 3.29e-26 105
MS.gene004150.t1 MTR_6g027460 51.042 96 45 2 4 98 59 153 5.53e-26 103
MS.gene004150.t1 MTR_5g041420 59.740 77 30 1 29 105 127 202 1.28e-25 101
MS.gene004150.t1 MTR_6g027450 46.809 94 49 1 5 98 54 146 1.36e-25 101
MS.gene004150.t1 MTR_6g012450 52.174 92 43 1 7 98 103 193 8.16e-25 101
MS.gene004150.t1 MTR_7g086780 59.722 72 28 1 27 98 86 156 2.03e-24 99.8
MS.gene004150.t1 MTR_4g082060 54.321 81 33 1 19 99 104 180 2.61e-24 99.8
MS.gene004150.t1 MTR_8g044220 47.059 102 40 3 29 128 107 196 7.77e-24 98.2
MS.gene004150.t1 MTR_2g016030 67.857 56 18 0 39 94 45 100 2.99e-23 91.7
MS.gene004150.t1 MTR_5g041380 59.211 76 27 1 25 100 115 186 2.04e-22 93.6
MS.gene004150.t1 MTR_5g041400 59.211 76 27 1 25 100 107 178 3.19e-22 92.8
MS.gene004150.t1 MTR_5g041530 58.571 70 28 1 25 94 125 193 2.52e-21 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004150.t1 AT2G28510 51.977 177 56 7 10 157 4 180 6.25e-43 145
MS.gene004150.t1 AT5G60200 45.263 190 71 6 25 182 40 228 1.63e-42 144
MS.gene004150.t1 AT3G45610 50.314 159 64 5 3 149 1 156 3.35e-39 135
MS.gene004150.t1 AT1G51700 63.158 95 31 2 21 114 16 107 2.58e-35 123
MS.gene004150.t1 AT3G55370 71.429 77 21 1 25 100 104 180 2.07e-33 123
MS.gene004150.t1 AT3G55370 71.429 77 21 1 25 100 59 135 2.71e-33 122
MS.gene004150.t1 AT3G55370 71.429 77 21 1 25 100 59 135 3.73e-33 122
MS.gene004150.t1 AT5G02460 73.611 72 18 1 25 95 78 149 2.02e-32 120
MS.gene004150.t1 AT1G21340 65.116 86 18 1 40 113 37 122 4.73e-32 117
MS.gene004150.t1 AT4G24060 72.727 66 18 0 30 95 42 107 5.22e-32 119
MS.gene004150.t1 AT1G07640 65.823 79 26 1 18 95 53 131 5.57e-32 118
MS.gene004150.t1 AT1G07640 65.823 79 26 1 18 95 61 139 7.53e-32 118
MS.gene004150.t1 AT3G52440 59.574 94 33 1 43 136 27 115 8.05e-32 116
MS.gene004150.t1 AT5G60850 72.000 75 19 1 39 111 49 123 9.60e-32 117
MS.gene004150.t1 AT3G52440 59.574 94 33 1 43 136 46 134 1.08e-31 116
MS.gene004150.t1 AT3G61850 52.066 121 52 3 27 147 49 163 1.13e-31 116
MS.gene004150.t1 AT3G61850 52.066 121 52 3 27 147 49 163 1.13e-31 116
MS.gene004150.t1 AT3G61850 52.066 121 52 3 27 147 61 175 1.33e-31 116
MS.gene004150.t1 AT2G37590 67.123 73 23 1 25 96 72 144 1.35e-31 117
MS.gene004150.t1 AT3G61850 52.066 121 52 3 27 147 49 163 1.56e-31 117
MS.gene004150.t1 AT3G61850 52.066 121 52 3 27 147 61 175 1.82e-31 117
MS.gene004150.t1 AT1G07640 69.444 72 21 1 25 95 4 75 2.33e-31 115
MS.gene004150.t1 AT2G28810 69.565 69 20 1 24 92 61 128 3.54e-31 116
MS.gene004150.t1 AT2G28810 69.565 69 20 1 24 92 78 145 5.67e-31 116
MS.gene004150.t1 AT5G62940 77.049 61 14 0 31 91 63 123 9.92e-31 115
MS.gene004150.t1 AT5G65590 80.328 61 12 0 41 101 41 101 1.19e-30 114
MS.gene004150.t1 AT1G28310 87.273 55 7 0 41 95 27 81 3.38e-30 113
MS.gene004150.t1 AT1G28310 58.947 95 39 0 1 95 11 105 4.88e-30 113
MS.gene004150.t1 AT3G21270 90.566 53 5 0 41 93 29 81 1.46e-29 109
MS.gene004150.t1 AT4G38000 72.727 66 17 1 30 94 30 95 3.77e-29 109
MS.gene004150.t1 AT4G21050 63.158 76 28 0 38 113 21 96 5.42e-29 107
MS.gene004150.t1 AT3G50410 72.308 65 18 0 33 97 22 86 8.14e-29 108
MS.gene004150.t1 AT4G00940 80.702 57 11 0 39 95 66 122 2.03e-28 108
MS.gene004150.t1 AT4G00940 80.702 57 11 0 39 95 66 122 2.03e-28 108
MS.gene004150.t1 AT4G00940 80.702 57 11 0 39 95 66 122 2.03e-28 108
MS.gene004150.t1 AT5G66940 61.905 84 25 2 37 113 28 111 2.07e-28 106
MS.gene004150.t1 AT1G64620 72.131 61 17 0 30 90 38 98 6.48e-28 108
MS.gene004150.t1 AT2G46590 63.158 76 24 1 13 88 44 115 1.05e-27 107
MS.gene004150.t1 AT2G46590 63.158 76 24 1 13 88 56 127 1.23e-27 107
MS.gene004150.t1 AT1G47655 75.439 57 14 0 33 89 21 77 2.16e-26 101
MS.gene004150.t1 AT5G62430 47.959 98 45 2 3 100 22 113 3.47e-26 102
MS.gene004150.t1 AT3G47500 52.632 95 41 1 4 98 77 167 5.27e-26 104
MS.gene004150.t1 AT5G39660 61.333 75 25 1 24 98 125 195 7.83e-25 101
MS.gene004150.t1 AT5G39660 61.333 75 25 1 24 98 125 195 7.83e-25 101
MS.gene004150.t1 AT5G39660 61.333 75 25 1 24 98 125 195 7.83e-25 101
MS.gene004150.t1 AT1G69570 53.488 86 36 1 9 94 104 185 1.46e-24 99.8
MS.gene004150.t1 AT4G21080 56.579 76 24 1 43 109 27 102 1.99e-24 97.1
MS.gene004150.t1 AT1G26790 55.263 76 33 1 20 94 89 164 5.52e-24 97.8
MS.gene004150.t1 AT4G21040 75.000 52 13 0 43 94 27 78 8.46e-24 94.7
MS.gene004150.t1 AT2G34140 66.071 56 19 0 39 94 56 111 4.54e-23 91.3
MS.gene004150.t1 AT1G29160 66.071 56 19 0 39 94 60 115 8.55e-23 90.9
MS.gene004150.t1 AT4G21030 51.471 68 33 0 38 105 18 85 1.59e-15 72.0

Find 57 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTTTGGTTTCCTAAAA+TGG 0.139828 4.2:-43144645 None:intergenic
GGGAAACAAGTGAGTGGATT+TGG 0.231178 4.2:-43144533 None:intergenic
GCTTCTGGTTGAGGAGGTTT+TGG 0.248273 4.2:-43144293 None:intergenic
GCAAGTCTTGTAGGAGATAT+TGG 0.255429 4.2:+43144399 MS.gene004150:CDS
GAATGGTCTCACTTCATCTT+GGG 0.274434 4.2:+43145012 MS.gene004150:CDS
GATATTTCTGCAAGTCTTGT+AGG 0.294030 4.2:+43144390 MS.gene004150:CDS
GGAATGGTCTCACTTCATCT+TGG 0.324184 4.2:+43145011 MS.gene004150:CDS
ATTGTTGTAGTAACAAAATT+TGG 0.325870 4.2:-43144348 None:intergenic
TTTCCATGGCAGTTCAATAA+TGG 0.330980 4.2:+43144944 MS.gene004150:CDS
GGAAACAAGTGAGTGGATTT+GGG 0.349121 4.2:-43144532 None:intergenic
ACGAGACGGTGTCTACATTA+GGG 0.358019 4.2:-43145056 None:intergenic
ATGGCTGAAATGGATCATTT+AGG 0.361202 4.2:+43144977 MS.gene004150:CDS
AGACACAAATATGGCTGAAA+TGG 0.367887 4.2:+43144967 MS.gene004150:CDS
CACGAGACGGTGTCTACATT+AGG 0.377042 4.2:-43145057 None:intergenic
CAATAATGGAGACACAAATA+TGG 0.378026 4.2:+43144958 MS.gene004150:CDS
CAAGACTTGCAGAAATATCT+TGG 0.387546 4.2:-43144386 None:intergenic
AGGAATGTTCCTGTTGGTGG+GGG 0.412807 4.2:+43144440 MS.gene004150:CDS
TTCTTCTAGTGGACAACAAC+AGG 0.413714 4.2:+43143501 MS.gene004150:CDS
TGTGAGATCATTGGAATCAT+AGG 0.425382 4.2:-43144564 None:intergenic
GGATTTGGGTTGGTTTGAAA+TGG 0.429582 4.2:-43144518 None:intergenic
TACCACATGAGGTGTTGGTT+TGG 0.441239 4.2:-43144658 None:intergenic
AGCATTAGCTAGGCTTCAAA+AGG 0.442460 4.2:+43144589 MS.gene004150:CDS
GCAAATGTCTAACCAGTCAT+TGG 0.467897 4.2:+43144229 MS.gene004150:intron
TGTGGTATTCTTAACAATCA+TGG 0.480447 4.2:+43144674 MS.gene004150:CDS
AAGAAGTGTTTGTATGGGGA+TGG 0.488483 4.2:+43143477 None:intergenic
AGGCTTCAAAAGGGTCAACT+TGG 0.489311 4.2:+43144599 MS.gene004150:CDS
ACAAGTGAGTGGATTTGGGT+TGG 0.491105 4.2:-43144528 None:intergenic
TAAGGAATGTTCCTGTTGGT+GGG 0.496140 4.2:+43144438 MS.gene004150:CDS
AATGGTCTCACTTCATCTTG+GGG 0.497543 4.2:+43145013 MS.gene004150:CDS
TGTAGGAGATATTGGACTAA+AGG 0.499003 4.2:+43144407 MS.gene004150:CDS
GTGTTGTTTGAGTCACATCT+TGG 0.500769 4.2:-43144326 None:intergenic
ATGGATCATTTAGGAAGAGC+TGG 0.502060 4.2:+43144986 MS.gene004150:CDS
TAATGCTAGTGTGAGATCAT+TGG 0.503293 4.2:-43144573 None:intergenic
TTGGAATCATAGGACATTGA+AGG 0.508739 4.2:-43144554 None:intergenic
TTAGGAAGAGCTGGTTGGAA+TGG 0.509850 4.2:+43144995 MS.gene004150:CDS
CTAAGGAATGTTCCTGTTGG+TGG 0.517333 4.2:+43144437 MS.gene004150:CDS
AAGAATACCACATGAGGTGT+TGG 0.521494 4.2:-43144663 None:intergenic
ATGGGGATGGATTCTTCTAG+TGG 0.553754 4.2:+43143490 MS.gene004150:CDS
TTTAGAGCTTCTGGTTGAGG+AGG 0.557206 4.2:-43144299 None:intergenic
AAGGAATGTTCCTGTTGGTG+GGG 0.558824 4.2:+43144439 MS.gene004150:CDS
TTAACATGCTCTCCAATGAC+TGG 0.564124 4.2:-43144241 None:intergenic
GGACTAAAGGTGGTACACTA+AGG 0.568077 4.2:+43144420 MS.gene004150:CDS
ATCATTTAGGAAGAGCTGGT+TGG 0.573027 4.2:+43144990 MS.gene004150:CDS
CGAGACGGTGTCTACATTAG+GGG 0.579103 4.2:-43145055 None:intergenic
TGGAATCATAGGACATTGAA+GGG 0.583945 4.2:-43144553 None:intergenic
GGAATGTTCCTGTTGGTGGG+GGG 0.588003 4.2:+43144441 MS.gene004150:CDS
ATCTCACACTAGCATTAGCT+AGG 0.588061 4.2:+43144579 MS.gene004150:CDS
CATCATCAGCTTCATCAAAG+AGG 0.595811 4.2:+43144483 MS.gene004150:CDS
AACCAAACCAACACCTCATG+TGG 0.607094 4.2:+43144656 MS.gene004150:CDS
ATGGTCTCACTTCATCTTGG+GGG 0.616298 4.2:+43145014 MS.gene004150:CDS
TCTCCATTATTGAACTGCCA+TGG 0.629912 4.2:-43144947 None:intergenic
GCATTAGCTAGGCTTCAAAA+GGG 0.630153 4.2:+43144590 MS.gene004150:CDS
GAGACGGTGTCTACATTAGG+GGG 0.640287 4.2:-43145054 None:intergenic
ATTGTTAAGAATACCACATG+AGG 0.643870 4.2:-43144669 None:intergenic
AGGAGATATTGGACTAAAGG+TGG 0.646558 4.2:+43144410 MS.gene004150:CDS
ACACTAAGGAATGTTCCTGT+TGG 0.660187 4.2:+43144434 MS.gene004150:CDS
ATTGAAGGGAAACAAGTGAG+TGG 0.662440 4.2:-43144539 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTCAGGAAAATAAT+AGG - chr4.2:43143667-43143686 None:intergenic 15.0%
!! TAACATTATGTTTGAATTAA+CGG + chr4.2:43143992-43144011 MS.gene004150:intron 15.0%
!! TCACAAAAATTTGTAATATT+TGG + chr4.2:43144167-43144186 MS.gene004150:intron 15.0%
!! ATTGTTGTAGTAACAAAATT+TGG - chr4.2:43144351-43144370 None:intergenic 20.0%
!! CAAAGAAGAAAAAAAAATTC+AGG - chr4.2:43143678-43143697 None:intergenic 20.0%
!! TTCCATCAAATATTAATTCT+AGG - chr4.2:43143825-43143844 None:intergenic 20.0%
!!! TTTACTTTATTTGATCTAGA+GGG - chr4.2:43143899-43143918 None:intergenic 20.0%
!!! TTTTACTTTATTTGATCTAG+AGG - chr4.2:43143900-43143919 None:intergenic 20.0%
! AACAAGAAATGTGTAATGTT+AGG + chr4.2:43144891-43144910 MS.gene004150:intron 25.0%
! ACAAGAAATGTGTAATGTTA+GGG + chr4.2:43144892-43144911 MS.gene004150:intron 25.0%
! ACATATGTATGTACTATGTA+TGG + chr4.2:43144139-43144158 MS.gene004150:intron 25.0%
! ATATTTGATGGAATAAAGTC+TGG + chr4.2:43143832-43143851 MS.gene004150:intron 25.0%
! GTAGTACTAAAAATTCATGA+AGG - chr4.2:43143624-43143643 None:intergenic 25.0%
! TAGGGAACAAAATGAAAATA+GGG + chr4.2:43144910-43144929 MS.gene004150:intron 25.0%
! TATTTGATGGAATAAAGTCT+GGG + chr4.2:43143833-43143852 MS.gene004150:intron 25.0%
! TTAGGGAACAAAATGAAAAT+AGG + chr4.2:43144909-43144928 MS.gene004150:intron 25.0%
!! GAACATGATTTTTCCATTTT+AGG + chr4.2:43144632-43144651 MS.gene004150:CDS 25.0%
!! TATTAATGGATGTACATGTT+GGG + chr4.2:43143936-43143955 MS.gene004150:intron 25.0%
!! TTACTTTATTTGATCTAGAG+GGG - chr4.2:43143898-43143917 None:intergenic 25.0%
!! TTATTAATGGATGTACATGT+TGG + chr4.2:43143935-43143954 MS.gene004150:intron 25.0%
!!! AATGAAAATAGGGTTTTGTT+AGG + chr4.2:43144920-43144939 MS.gene004150:intron 25.0%
!!! ACTAATTTTATTTCACCCAA+GGG - chr4.2:43143708-43143727 None:intergenic 25.0%
!!! CATGAATTTTTAGTACTACT+TGG + chr4.2:43143625-43143644 MS.gene004150:intron 25.0%
!!! TACTAATTTTATTTCACCCA+AGG - chr4.2:43143709-43143728 None:intergenic 25.0%
AAATTCATGAAGGACAAAGA+TGG - chr4.2:43143614-43143633 None:intergenic 30.0%
ACACAACACAACAAATTTAG+TGG - chr4.2:43144202-43144221 None:intergenic 30.0%
ATGCTCATTTCATGATCATA+AGG - chr4.2:43144836-43144855 None:intergenic 30.0%
ATTGTTAAGAATACCACATG+AGG - chr4.2:43144672-43144691 None:intergenic 30.0%
GAATCAGAAAAGTTACATTG+TGG + chr4.2:43144026-43144045 MS.gene004150:intron 30.0%
GGCCTAGAATTAATATTTGA+TGG + chr4.2:43143820-43143839 MS.gene004150:intron 30.0%
TGAATTAACGGTGAATTTGT+CGG + chr4.2:43144004-43144023 MS.gene004150:intron 30.0%
TGTGGTATTCTTAACAATCA+TGG + chr4.2:43144674-43144693 MS.gene004150:CDS 30.0%
! CAATAATGGAGACACAAATA+TGG + chr4.2:43144958-43144977 MS.gene004150:CDS 30.0%
AATGCTAATGGCAATGGTAA+TGG + chr4.2:43144797-43144816 MS.gene004150:intron 35.0%
ACTACTTGGAAGAATGAACA+AGG + chr4.2:43143639-43143658 MS.gene004150:intron 35.0%
AGACACAAATATGGCTGAAA+TGG + chr4.2:43144967-43144986 MS.gene004150:CDS 35.0%
ATGGCTGAAATGGATCATTT+AGG + chr4.2:43144977-43144996 MS.gene004150:CDS 35.0%
CAAGACTTGCAGAAATATCT+TGG - chr4.2:43144389-43144408 None:intergenic 35.0%
GTGCAGAATCTTTACTACAT+GGG + chr4.2:43144758-43144777 MS.gene004150:intron 35.0%
TAATGCTAGTGTGAGATCAT+TGG - chr4.2:43144576-43144595 None:intergenic 35.0%
TGCAGAATCTTTACTACATG+GGG + chr4.2:43144759-43144778 MS.gene004150:intron 35.0%
TGGAATCATAGGACATTGAA+GGG - chr4.2:43144556-43144575 None:intergenic 35.0%
TGTAGGAGATATTGGACTAA+AGG + chr4.2:43144407-43144426 MS.gene004150:CDS 35.0%
TGTGAGATCATTGGAATCAT+AGG - chr4.2:43144567-43144586 None:intergenic 35.0%
TGTGCAGAATCTTTACTACA+TGG + chr4.2:43144757-43144776 MS.gene004150:intron 35.0%
TTAGCATCTTCATCTTTGTG+AGG + chr4.2:43143872-43143891 MS.gene004150:intron 35.0%
TTGGAATCATAGGACATTGA+AGG - chr4.2:43144557-43144576 None:intergenic 35.0%
TTTCCATGGCAGTTCAATAA+TGG + chr4.2:43144944-43144963 MS.gene004150:CDS 35.0%
! GATATTTCTGCAAGTCTTGT+AGG + chr4.2:43144390-43144409 MS.gene004150:CDS 35.0%
!! GTTGATAATGGCAATGCTAA+TGG + chr4.2:43144785-43144804 MS.gene004150:intron 35.0%
!! GTTGGTTTGGTTTCCTAAAA+TGG - chr4.2:43144648-43144667 None:intergenic 35.0%
AACCATTCCTCAAACAACCA+TGG + chr4.2:43144701-43144720 MS.gene004150:intron 40.0%
AAGAATACCACATGAGGTGT+TGG - chr4.2:43144666-43144685 None:intergenic 40.0%
AATGGCAATGCTAATGGCAA+TGG + chr4.2:43144791-43144810 MS.gene004150:intron 40.0%
AATGGTCTCACTTCATCTTG+GGG + chr4.2:43145013-43145032 MS.gene004150:CDS 40.0%
ACACTAAGGAATGTTCCTGT+TGG + chr4.2:43144434-43144453 MS.gene004150:CDS 40.0%
AGCATTAGCTAGGCTTCAAA+AGG + chr4.2:43144589-43144608 MS.gene004150:CDS 40.0%
AGGAGATATTGGACTAAAGG+TGG + chr4.2:43144410-43144429 MS.gene004150:CDS 40.0%
ATCATTTAGGAAGAGCTGGT+TGG + chr4.2:43144990-43145009 MS.gene004150:CDS 40.0%
ATCCATGGTTGTTTGAGGAA+TGG - chr4.2:43144706-43144725 None:intergenic 40.0%
ATCTCACACTAGCATTAGCT+AGG + chr4.2:43144579-43144598 MS.gene004150:CDS 40.0%
ATGAACCACATGTATACAGC+TGG + chr4.2:43143797-43143816 MS.gene004150:intron 40.0%
ATGGATCATTTAGGAAGAGC+TGG + chr4.2:43144986-43145005 MS.gene004150:CDS 40.0%
ATTGAAGGGAAACAAGTGAG+TGG - chr4.2:43144542-43144561 None:intergenic 40.0%
CATCATCAGCTTCATCAAAG+AGG + chr4.2:43144483-43144502 MS.gene004150:CDS 40.0%
GAAGAATCCATGGTTGTTTG+AGG - chr4.2:43144711-43144730 None:intergenic 40.0%
GAATGGTCTCACTTCATCTT+GGG + chr4.2:43145012-43145031 MS.gene004150:CDS 40.0%
GCAAATGTCTAACCAGTCAT+TGG + chr4.2:43144229-43144248 MS.gene004150:intron 40.0%
GCAGAATCTTTACTACATGG+GGG + chr4.2:43144760-43144779 MS.gene004150:intron 40.0%
GCATTAGCTAGGCTTCAAAA+GGG + chr4.2:43144590-43144609 MS.gene004150:CDS 40.0%
GCTGTATACATGTGGTTCAT+AGG - chr4.2:43143797-43143816 None:intergenic 40.0%
TAAGGAATGTTCCTGTTGGT+GGG + chr4.2:43144438-43144457 MS.gene004150:CDS 40.0%
TAAGTGCTTCGAAGAATCCA+TGG - chr4.2:43144721-43144740 None:intergenic 40.0%
TCATGAAGGACAAAGATGGA+AGG - chr4.2:43143610-43143629 None:intergenic 40.0%
TCTCCATTATTGAACTGCCA+TGG - chr4.2:43144950-43144969 None:intergenic 40.0%
TGAACCACATGTATACAGCT+GGG + chr4.2:43143798-43143817 MS.gene004150:intron 40.0%
TTAACATGCTCTCCAATGAC+TGG - chr4.2:43144244-43144263 None:intergenic 40.0%
! CTTCTTTGAAGAACACCCTT+GGG + chr4.2:43143690-43143709 MS.gene004150:intron 40.0%
! GCAAGTCTTGTAGGAGATAT+TGG + chr4.2:43144399-43144418 MS.gene004150:CDS 40.0%
! GGAAACAAGTGAGTGGATTT+GGG - chr4.2:43144535-43144554 None:intergenic 40.0%
! GGATTCTTCGAAGCACTTAT+GGG + chr4.2:43144722-43144741 MS.gene004150:intron 40.0%
! TCTTCTTTGAAGAACACCCT+TGG + chr4.2:43143689-43143708 MS.gene004150:intron 40.0%
! TGGATTCTTCGAAGCACTTA+TGG + chr4.2:43144721-43144740 MS.gene004150:intron 40.0%
! TTCTTCTAGTGGACAACAAC+AGG + chr4.2:43143501-43143520 MS.gene004150:CDS 40.0%
!! GGATTTGGGTTGGTTTGAAA+TGG - chr4.2:43144521-43144540 None:intergenic 40.0%
!! GTGTTGTTTGAGTCACATCT+TGG - chr4.2:43144329-43144348 None:intergenic 40.0%
!!! CATTTTAGAGCTTCTGGTTG+AGG - chr4.2:43144305-43144324 None:intergenic 40.0%
!!! CTTGGACATTTTAGAGCTTC+TGG - chr4.2:43144311-43144330 None:intergenic 40.0%
!!! GGGTTTTGTTAGGTTTTCCA+TGG + chr4.2:43144930-43144949 MS.gene004150:intron 40.0%
AACCAAACCAACACCTCATG+TGG + chr4.2:43144656-43144675 MS.gene004150:CDS 45.0%
AAGGAATGTTCCTGTTGGTG+GGG + chr4.2:43144439-43144458 MS.gene004150:CDS 45.0%
ACATTAGGGGGCTATTGAGA+AGG - chr4.2:43145045-43145064 None:intergenic 45.0%
AGGCTTCAAAAGGGTCAACT+TGG + chr4.2:43144599-43144618 MS.gene004150:CDS 45.0%
ATGGTCTCACTTCATCTTGG+GGG + chr4.2:43145014-43145033 MS.gene004150:CDS 45.0%
CTAAGGAATGTTCCTGTTGG+TGG + chr4.2:43144437-43144456 MS.gene004150:CDS 45.0%
GAACCACATGTATACAGCTG+GGG + chr4.2:43143799-43143818 MS.gene004150:intron 45.0%
GAATCTTTACTACATGGGGG+AGG + chr4.2:43144763-43144782 MS.gene004150:intron 45.0%
GGAATGGTCTCACTTCATCT+TGG + chr4.2:43145011-43145030 MS.gene004150:CDS 45.0%
GGACTAAAGGTGGTACACTA+AGG + chr4.2:43144420-43144439 MS.gene004150:CDS 45.0%
TTAGGAAGAGCTGGTTGGAA+TGG + chr4.2:43144995-43145014 MS.gene004150:CDS 45.0%
! ACAAGTGAGTGGATTTGGGT+TGG - chr4.2:43144531-43144550 None:intergenic 45.0%
! ATGGGGATGGATTCTTCTAG+TGG + chr4.2:43143490-43143509 MS.gene004150:CDS 45.0%
! GGGAAACAAGTGAGTGGATT+TGG - chr4.2:43144536-43144555 None:intergenic 45.0%
! TTTAGAGCTTCTGGTTGAGG+AGG - chr4.2:43144302-43144321 None:intergenic 45.0%
!! TACATGGGGGAGGTTGATAA+TGG + chr4.2:43144773-43144792 MS.gene004150:intron 45.0%
!! TACCACATGAGGTGTTGGTT+TGG - chr4.2:43144661-43144680 None:intergenic 45.0%
!! AAAAATTGAAAATTTATTAA+TGG + chr4.2:43143922-43143941 MS.gene004150:intron 5.0%
! AGGAATGTTCCTGTTGGTGG+GGG + chr4.2:43144440-43144459 MS.gene004150:CDS 50.0%
!! GCTTCTGGTTGAGGAGGTTT+TGG - chr4.2:43144296-43144315 None:intergenic 50.0%
AGGCCCCAGCTGTATACATG+TGG - chr4.2:43143805-43143824 None:intergenic 55.0%
CTCACTTCATCTTGGGGGCA+TGG + chr4.2:43145019-43145038 MS.gene004150:CDS 55.0%
TTTCTACACCCCCCACCAAC+AGG - chr4.2:43144452-43144471 None:intergenic 55.0%
! GGAATGTTCCTGTTGGTGGG+GGG + chr4.2:43144441-43144460 MS.gene004150:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 43143490 43145066 43143490 ID=MS.gene004150
chr4.2 mRNA 43143490 43145066 43143490 ID=MS.gene004150.t1;Parent=MS.gene004150
chr4.2 exon 43143490 43143522 43143490 ID=MS.gene004150.t1.exon1;Parent=MS.gene004150.t1
chr4.2 CDS 43143490 43143522 43143490 ID=cds.MS.gene004150.t1;Parent=MS.gene004150.t1
chr4.2 exon 43144230 43144695 43144230 ID=MS.gene004150.t1.exon2;Parent=MS.gene004150.t1
chr4.2 CDS 43144230 43144695 43144230 ID=cds.MS.gene004150.t1;Parent=MS.gene004150.t1
chr4.2 exon 43144942 43145066 43144942 ID=MS.gene004150.t1.exon3;Parent=MS.gene004150.t1
chr4.2 CDS 43144942 43145066 43144942 ID=cds.MS.gene004150.t1;Parent=MS.gene004150.t1
Gene Sequence

>MS.gene004150

ATGGGGATGGATTCTTCTAGTGGACAACAACAGGTGAAATATTTATTTCATATCATATGTAATTATATCATGCATGTAGTGATGTAATATTTATTAACGTGACTTCTTTGATTTTTGCCTTCCATCTTTGTCCTTCATGAATTTTTAGTACTACTTGGAAGAATGAACAAGGAACCTATTATTTTCCTGAATTTTTTTTTCTTCTTTGAAGAACACCCTTGGGTGAAATAAAATTAGTAAAGCTATGTCTCTCTACAATTTTATTATGTACTATATAGCATATACTATACTACTCATAGTGTTTCCTATGAACCACATGTATACAGCTGGGGCCTAGAATTAATATTTGATGGAATAAAGTCTGGGAGAATAGAAAAAAAAATTAGCATCTTCATCTTTGTGAGGCCCCTCTAGATCAAATAAAGTAAAAAGAAAAATTGAAAATTTATTAATGGATGTACATGTTGGGAGTGAGTGATTTATTCAGTTTCGTTTTAGTTCTTAACATTATGTTTGAATTAACGGTGAATTTGTCGGAATCAGAAAAGTTACATTGTGGAGTTTCAATAATACTAAGACGACACACAATTTTGTCAAATTCGCAATGATTTCAAAAATCAATTAACTCTTTGATTTTTTACTTGTGTACACATATGTATGTACTATGTATGGATAGATCACAAAAATTTGTAATATTTGGTTTTCATGTCCACTAAATTTGTTGTGTTGTGTTGTTGCAGCAAATGTCTAACCAGTCATTGGAGAGCATGTTAACATGTTCAAAAGCAGAACAAGACAAAAAGCCAAAACCTCCTCAACCAGAAGCTCTAAAATGTCCAAGATGTGACTCAAACAACACCAAATTTTGTTACTACAACAATTACAGTCTTTCTCAACCAAGATATTTCTGCAAGTCTTGTAGGAGATATTGGACTAAAGGTGGTACACTAAGGAATGTTCCTGTTGGTGGGGGGTGTAGAAAGAACAAAAGATCATCATCAGCTTCATCAAAGAGGTCTCAAGATCAACCATTTCAAACCAACCCAAATCCACTCACTTGTTTCCCTTCAATGTCCTATGATTCCAATGATCTCACACTAGCATTAGCTAGGCTTCAAAAGGGTCAACTTGGATTTGATCATGAACATGATTTTTCCATTTTAGGAAACCAAACCAACACCTCATGTGGTATTCTTAACAATCATGGTATGAACCATTCCTCAAACAACCATGGATTCTTCGAAGCACTTATGGGATCTCAAAACAATGTGCAGAATCTTTACTACATGGGGGAGGTTGATAATGGCAATGCTAATGGCAATGGTAATGGTGAGATGATGTTACCTTATGATCATGAAATGAGCATTGCAACTACTCAAGCAGTTACAGTTACTACTATGAAACAAGAAATGTGTAATGTTAGGGAACAAAATGAAAATAGGGTTTTGTTAGGTTTTCCATGGCAGTTCAATAATGGAGACACAAATATGGCTGAAATGGATCATTTAGGAAGAGCTGGTTGGAATGGTCTCACTTCATCTTGGGGGCATGGCCTTCTCAATAGCCCCCTAATGTAG
Protein sequence

>MS.gene004150.t1

MGMDSSSGQQQQMSNQSLESMLTCSKAEQDKKPKPPQPEALKCPRCDSNNTKFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGGCRKNKRSSSASSKRSQDQPFQTNPNPLTCFPSMSYDSNDLTLALARLQKGQLGFDHEHDFSILGNQTNTSCGILNNHGFPWQFNNGDTNMAEMDHLGRAGWNGLTSSWGHGLLNSPLM