AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004153

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004153.t1 MTR_7g074790 56.180 178 59 2 1 177 1 160 1.61e-61 202
MS.gene004153.t1 MTR_5g039760 50.510 196 71 3 1 194 1 172 6.50e-58 191
MS.gene004153.t1 MTR_7g061980 57.143 119 50 1 1 118 1 119 8.15e-43 149
MS.gene004153.t1 MTR_0402s0040 28.425 489 283 14 10 482 26 463 2.24e-37 144
MS.gene004153.t1 MTR_4g102720 35.119 168 76 1 7 174 4 138 6.25e-31 120
MS.gene004153.t1 MTR_2g099430 48.276 87 44 1 33 118 26 112 1.11e-19 85.1
MS.gene004153.t1 MTR_4g069880 51.562 64 31 0 1 64 1 64 1.86e-17 77.0
MS.gene004153.t1 MTR_5g015480 40.650 123 68 4 10 128 56 177 2.70e-17 82.4
MS.gene004153.t1 MTR_7g113010 37.302 126 64 2 10 133 457 569 2.66e-16 82.4
MS.gene004153.t1 MTR_1g019850 31.579 133 83 3 10 140 70 196 7.53e-16 77.4
MS.gene004153.t1 MTR_1g083640 30.052 193 109 6 8 178 52 240 3.07e-15 76.6
MS.gene004153.t1 MTR_0558s0020 30.052 193 109 6 8 178 37 225 4.32e-15 76.3
MS.gene004153.t1 MTR_1g070355 28.571 168 110 3 14 174 78 242 6.21e-15 77.8
MS.gene004153.t1 MTR_3g058090 36.585 123 54 3 327 449 5 103 1.08e-14 70.5
MS.gene004153.t1 MTR_1g057920 40.449 89 50 2 10 95 34 122 5.75e-14 68.9
MS.gene004153.t1 MTR_2g014100 29.534 193 110 6 8 178 45 233 6.89e-14 72.0
MS.gene004153.t1 MTR_6g017200 39.024 82 49 1 10 90 58 139 1.44e-13 68.9
MS.gene004153.t1 MTR_1g013060 30.000 170 112 3 7 169 21 190 2.04e-13 70.1
MS.gene004153.t1 MTR_5g045210 32.381 105 68 2 329 432 20 122 2.28e-13 69.3
MS.gene004153.t1 MTR_5g076070 29.375 160 109 3 13 168 56 215 1.06e-12 68.9
MS.gene004153.t1 MTR_4g117620 62.791 43 16 0 485 527 36 78 4.17e-12 62.4
MS.gene004153.t1 MTR_0337s0060 24.885 217 129 4 181 397 18 200 4.16e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004153.t1 AT4G15090 29.537 281 180 7 184 453 442 715 6.50e-32 131
MS.gene004153.t1 AT4G15090 29.121 182 121 5 3 179 44 222 4.49e-13 72.4
MS.gene004153.t1 AT4G15090 29.537 281 180 7 184 453 442 715 6.50e-32 131
MS.gene004153.t1 AT4G15090 29.121 182 121 5 3 179 44 222 4.49e-13 72.4
MS.gene004153.t1 AT4G15090 29.537 281 180 7 184 453 442 715 6.50e-32 131
MS.gene004153.t1 AT4G15090 29.121 182 121 5 3 179 44 222 4.49e-13 72.4
MS.gene004153.t1 AT4G15090 29.537 281 180 7 184 453 442 715 6.50e-32 131
MS.gene004153.t1 AT4G15090 29.121 182 121 5 3 179 44 222 4.49e-13 72.4
MS.gene004153.t1 AT4G15090 29.537 281 180 7 184 453 442 715 6.50e-32 131
MS.gene004153.t1 AT4G15090 29.121 182 121 5 3 179 44 222 4.49e-13 72.4
MS.gene004153.t1 AT4G38180 31.429 210 139 3 185 390 467 675 3.76e-23 104
MS.gene004153.t1 AT4G38180 32.222 180 117 3 6 180 69 248 3.25e-21 98.2
MS.gene004153.t1 AT4G38170 28.692 237 147 6 185 411 232 456 1.74e-20 95.1
MS.gene004153.t1 AT3G22170 28.767 219 145 3 184 400 451 660 2.21e-20 95.5
MS.gene004153.t1 AT3G22170 28.767 219 145 3 184 400 451 660 2.21e-20 95.5
MS.gene004153.t1 AT1G76320 32.203 177 116 3 178 353 372 545 2.70e-19 92.0
MS.gene004153.t1 AT1G76320 32.203 177 116 3 178 353 372 545 2.70e-19 92.0
MS.gene004153.t1 AT1G76320 32.203 177 116 3 178 353 372 545 2.92e-19 92.0
MS.gene004153.t1 AT1G76320 32.203 177 116 3 178 353 372 545 4.46e-19 91.3
MS.gene004153.t1 AT4G19990 27.731 357 219 10 178 502 383 732 1.45e-18 89.7
MS.gene004153.t1 AT4G19990 27.731 357 219 10 178 502 383 732 1.45e-18 89.7
MS.gene004153.t1 AT4G19990 26.891 357 222 9 178 502 383 732 1.86e-18 89.4
MS.gene004153.t1 AT4G19990 26.891 357 222 9 178 502 383 732 1.86e-18 89.4
MS.gene004153.t1 AT4G19990 26.891 357 222 9 178 502 383 732 2.01e-18 89.4
MS.gene004153.t1 AT4G19990 26.891 357 222 9 178 502 326 675 2.17e-18 89.4
MS.gene004153.t1 AT4G19990 26.891 357 222 9 178 502 397 746 2.27e-18 89.4
MS.gene004153.t1 AT5G18960 29.442 197 137 2 190 385 566 761 6.91e-17 84.7
MS.gene004153.t1 AT5G18960 42.718 103 44 4 9 109 42 131 2.89e-14 76.3
MS.gene004153.t1 AT5G18960 29.442 197 137 2 190 385 566 761 6.91e-17 84.7
MS.gene004153.t1 AT5G18960 42.718 103 44 4 9 109 42 131 2.89e-14 76.3
MS.gene004153.t1 AT3G06250 31.818 154 104 1 233 385 584 737 8.80e-17 84.3
MS.gene004153.t1 AT3G06250 38.835 103 48 4 9 109 27 116 4.43e-12 69.3
MS.gene004153.t1 AT3G06250 31.818 154 104 1 233 385 584 737 8.80e-17 84.3
MS.gene004153.t1 AT3G06250 38.835 103 48 4 9 109 27 116 4.43e-12 69.3
MS.gene004153.t1 AT3G06250 31.818 154 104 1 233 385 584 737 8.80e-17 84.3
MS.gene004153.t1 AT3G06250 38.835 103 48 4 9 109 27 116 4.43e-12 69.3
MS.gene004153.t1 AT2G32250 24.891 229 154 5 184 404 345 563 2.06e-15 79.7
MS.gene004153.t1 AT2G32250 24.891 229 154 5 184 404 386 604 2.08e-15 79.7
MS.gene004153.t1 AT2G32250 24.891 229 154 5 184 404 386 604 2.10e-15 79.7
MS.gene004153.t1 AT2G32250 24.891 229 154 5 184 404 386 604 2.10e-15 79.7
MS.gene004153.t1 AT2G32250 24.891 229 154 5 184 404 345 563 2.12e-15 79.7
MS.gene004153.t1 AT2G32250 24.891 229 154 5 184 404 386 604 2.39e-15 79.7
MS.gene004153.t1 AT2G32250 24.891 229 154 5 184 404 386 604 2.39e-15 79.7
MS.gene004153.t1 AT2G32250 24.891 229 154 5 184 404 386 604 2.39e-15 79.7
MS.gene004153.t1 AT2G27110 29.187 209 136 4 211 411 293 497 2.43e-14 76.3
MS.gene004153.t1 AT2G27110 29.187 209 136 4 211 411 438 642 2.74e-14 76.3
MS.gene004153.t1 AT2G27110 29.187 209 136 4 211 411 438 642 2.74e-14 76.3
MS.gene004153.t1 AT5G28530 28.402 169 115 4 8 170 53 221 2.49e-13 73.2
MS.gene004153.t1 AT5G28530 28.402 169 115 4 8 170 53 221 2.57e-13 73.2
MS.gene004153.t1 AT5G28530 28.402 169 115 4 8 170 53 221 3.17e-13 72.8
MS.gene004153.t1 AT5G28530 28.402 169 115 4 8 170 53 221 3.17e-13 72.8
MS.gene004153.t1 AT5G28530 28.402 169 115 4 8 170 53 221 3.41e-13 72.8

Find 79 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAACACTATCCAATATTT+TGG 0.149102 4.2:+43163029 None:intergenic
ATCTTGTATGCAACCGAATT+TGG 0.167798 4.2:-43163315 MS.gene004153:CDS
TAGATGAAGTTCATCAATTT+TGG 0.174539 4.2:-43164500 MS.gene004153:CDS
ATTGATTTGGCTAATGATTC+TGG 0.243805 4.2:-43164162 MS.gene004153:CDS
ATGTGAATATGTCATTACTA+TGG 0.270929 4.2:-43163060 MS.gene004153:CDS
CAGTTTCAAGTAATGTTTCT+AGG 0.273436 4.2:-43162623 MS.gene004153:CDS
GTTGTCTCAAATTTCATAAA+AGG 0.301460 4.2:-43162777 MS.gene004153:CDS
TATGCTTATCTTCAAATTCA+TGG 0.305536 4.2:+43164262 None:intergenic
TGTAGAAAGTTTGAAACATT+TGG 0.316097 4.2:-43162975 MS.gene004153:CDS
TGGCTTCCAATCAACATTTA+CGG 0.338693 4.2:+43164548 None:intergenic
ATTTGTCGTGGCTCATAAAC+TGG 0.340582 4.2:+43162426 None:intergenic
GTGGCGTTGTGCAAGATGTT+AGG 0.347695 4.2:-43162854 MS.gene004153:CDS
GTCATGAATGCCGTTGTCTT+TGG 0.359484 4.2:+43162659 None:intergenic
TATGCTGCACGTACTGGCTT+TGG 0.360264 4.2:-43164471 MS.gene004153:CDS
CTTGCTTATGCTGCACGTAC+TGG 0.365082 4.2:-43164477 MS.gene004153:CDS
GTGTGTATACTTTCCCCATT+TGG 0.367247 4.2:+43163165 None:intergenic
AATTGCATGATTTCCAAATT+CGG 0.367504 4.2:+43163302 None:intergenic
TAGCTTAACAGCGTATTTGA+TGG 0.383182 4.2:-43162388 MS.gene004153:intron
TGTCATGCGTAAGAAAATAA+TGG 0.385423 4.2:-43162697 MS.gene004153:CDS
TGACTACATGGTTGCTCTCT+AGG 0.386661 4.2:+43162498 None:intergenic
TAAAAGAAGACAAAGAAGTA+TGG 0.394624 4.2:-43164043 MS.gene004153:intron
ACAAGCACATGAGATTGATT+TGG 0.396994 4.2:-43164175 MS.gene004153:CDS
CTTCGTGTTCTAGACAACTT+TGG 0.403555 4.2:+43162810 None:intergenic
AAACATTACTTGAAACTGTT+GGG 0.416514 4.2:+43162628 None:intergenic
TCAAATTTCATTCCAACCCT+TGG 0.427925 4.2:+43164528 None:intergenic
TTCCCCATTTGGATCAGTAT+TGG 0.432896 4.2:+43163176 None:intergenic
CTTCTTAAAATATACTTCTC+AGG 0.433030 4.2:+43162897 None:intergenic
ATGTAGATTTGTTTGTTGTA+AGG 0.434039 4.2:-43164400 MS.gene004153:CDS
GAAACATTACTTGAAACTGT+TGG 0.441612 4.2:+43162627 None:intergenic
TGACATCCGTAAATGTTGAT+TGG 0.442141 4.2:-43164554 MS.gene004153:CDS
TGTGAATATGTCATTACTAT+GGG 0.443172 4.2:-43163059 MS.gene004153:CDS
GCAAGCATGTGTGTTGTCTC+TGG 0.449397 4.2:+43164222 None:intergenic
TGGCGTTGTGCAAGATGTTA+GGG 0.450029 4.2:-43162853 MS.gene004153:CDS
TCACCAATACTGATCCAAAT+GGG 0.459888 4.2:-43163179 MS.gene004153:CDS
ACTTGAGACACTTGACTACA+TGG 0.464898 4.2:+43162486 None:intergenic
ATCTTGCACAACGCCACTTC+GGG 0.465364 4.2:+43162860 None:intergenic
ATCACACTTACACGTTATGA+TGG 0.466378 4.2:-43164297 MS.gene004153:CDS
AGATTTGTTTGTTGTAAGGA+AGG 0.482622 4.2:-43164396 MS.gene004153:CDS
TAAAATATACTTCTCAGGAA+TGG 0.483384 4.2:+43162902 None:intergenic
GTCACCAATACTGATCCAAA+TGG 0.487378 4.2:-43163180 MS.gene004153:CDS
CATCTTGCACAACGCCACTT+CGG 0.498150 4.2:+43162859 None:intergenic
AGTTTCAAGTAATGTTTCTA+GGG 0.511152 4.2:-43162622 MS.gene004153:CDS
AGTGTCTCAAGTATATCATA+TGG 0.522885 4.2:-43162475 MS.gene004153:CDS
GGTCGATGGAGTATCTTGTT+CGG 0.535349 4.2:-43162454 MS.gene004153:CDS
CACAAAAGCGACATAAGAGT+TGG 0.536198 4.2:-43162557 MS.gene004153:CDS
CAACTTATGAGCACACATGC+AGG 0.539062 4.2:-43164117 MS.gene004153:CDS
GTTGATTGGAAGCCAAGGGT+TGG 0.540661 4.2:-43164540 MS.gene004153:CDS
TACACGTTATGATGGGAAGT+TGG 0.549441 4.2:-43164289 MS.gene004153:CDS
CAAGTATATCATATGGTCGA+TGG 0.554858 4.2:-43162468 MS.gene004153:CDS
TATGAGCCACGACAAATACA+AGG 0.558713 4.2:-43162420 MS.gene004153:CDS
TGAGTCCCTTGTATTTGTCG+TGG 0.560216 4.2:+43162414 None:intergenic
CTTTCTACAACTACAAGCAA+TGG 0.561499 4.2:+43162989 None:intergenic
GATTTGTTTGTTGTAAGGAA+GGG 0.580884 4.2:-43164395 MS.gene004153:CDS
CTACATGGTTGCTCTCTAGG+TGG 0.583984 4.2:+43162501 None:intergenic
AGTCCTCACATAAGCTTGCA+AGG 0.585698 4.2:-43163217 MS.gene004153:CDS
AACTTATGAGCACACATGCA+GGG 0.592671 4.2:-43164116 MS.gene004153:CDS
AAATGTTGATTGGAAGCCAA+GGG 0.593917 4.2:-43164544 MS.gene004153:CDS
GCAGGGCATAGATCTAATGT+TGG 0.596510 4.2:-43164099 MS.gene004153:CDS
TAAATGTTGATTGGAAGCCA+AGG 0.602713 4.2:-43164545 MS.gene004153:CDS
GTGAATATGTCATTACTATG+GGG 0.604891 4.2:-43163058 MS.gene004153:CDS
GCAAGGTCTGTCTCACTAAG+TGG 0.604947 4.2:+43162273 None:intergenic
TTTCATAAAAGGTGCAGCTG+AGG 0.605578 4.2:-43162766 MS.gene004153:CDS
TGAGGCATCACCTTGTGAAG+AGG 0.612403 4.2:-43162748 MS.gene004153:CDS
TCACACTTACACGTTATGAT+GGG 0.613020 4.2:-43164296 MS.gene004153:CDS
ATGAAGAAAGCAGTAACACT+TGG 0.614713 4.2:-43163383 MS.gene004153:intron
CCAATCAGTGTAATGCTGCA+AGG 0.621517 4.2:+43162256 None:intergenic
CGGTCAAACGCCAAAGACAA+CGG 0.631949 4.2:-43162669 MS.gene004153:CDS
TCTCTGGAGATAGCAGCTCA+TGG 0.634385 4.2:+43164238 None:intergenic
TCTACAACTACAAGCAATGG+AGG 0.636457 4.2:+43162992 None:intergenic
TGAATATGTCATTACTATGG+GGG 0.641434 4.2:-43163057 MS.gene004153:CDS
TAATGTTGGATTTACACGAG+TGG 0.643534 4.2:-43164085 MS.gene004153:CDS
CACCAATACTGATCCAAATG+GGG 0.658495 4.2:-43163178 MS.gene004153:CDS
GGCGTTGTGCAAGATGTTAG+GGG 0.670016 4.2:-43162852 MS.gene004153:CDS
TAACCTTGCAAGCTTATGTG+AGG 0.674762 4.2:+43163214 None:intergenic
TGGACAAGAGATGCCCGAAG+TGG 0.678714 4.2:-43162873 MS.gene004153:CDS
GCGTTGTGCAAGATGTTAGG+GGG 0.680564 4.2:-43162851 MS.gene004153:CDS
TGTCGTGGCTCATAAACTGG+AGG 0.687831 4.2:+43162429 None:intergenic
GTAAGAAAATAATGGAACGT+CGG 0.713792 4.2:-43162689 MS.gene004153:CDS
ATGAGCCACGACAAATACAA+GGG 0.736791 4.2:-43162419 MS.gene004153:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATGCTATTAAAAAAAAT+GGG - chr4.2:43163398-43163417 MS.gene004153:intron 10.0%
!!! TTTTTAAAAAAAAATTATGC+AGG - chr4.2:43164517-43164536 MS.gene004153:CDS 10.0%
!! GTTTATGCTATTAAAAAAAA+TGG - chr4.2:43163397-43163416 MS.gene004153:intron 15.0%
!!! AATAACACTATCCAATATTT+TGG + chr4.2:43163789-43163808 None:intergenic 20.0%
!!! AATTCTTCAAACATTTTGAA+CGG - chr4.2:43163532-43163551 MS.gene004153:intron 20.0%
! AAACATTACTTGAAACTGTT+GGG + chr4.2:43164190-43164209 None:intergenic 25.0%
! AATTGCATGATTTCCAAATT+CGG + chr4.2:43163516-43163535 None:intergenic 25.0%
! ATGTGAATATGTCATTACTA+TGG - chr4.2:43163755-43163774 MS.gene004153:intron 25.0%
! CTTCTTAAAATATACTTCTC+AGG + chr4.2:43163921-43163940 None:intergenic 25.0%
! GTTGTCTCAAATTTCATAAA+AGG - chr4.2:43164038-43164057 MS.gene004153:intron 25.0%
! TAAAAGAAGACAAAGAAGTA+TGG - chr4.2:43162772-43162791 MS.gene004153:CDS 25.0%
! TAAAATATACTTCTCAGGAA+TGG + chr4.2:43163916-43163935 None:intergenic 25.0%
! TATGCGAACAAAAATAAAGA+CGG - chr4.2:43162380-43162399 MS.gene004153:intron 25.0%
! TATGCTTATCTTCAAATTCA+TGG + chr4.2:43162556-43162575 None:intergenic 25.0%
! TGTGAATATGTCATTACTAT+GGG - chr4.2:43163756-43163775 MS.gene004153:intron 25.0%
!! AACAAATGACAAATGTGTTT+TGG - chr4.2:43162888-43162907 MS.gene004153:CDS 25.0%
!! AGTTTCAAGTAATGTTTCTA+GGG - chr4.2:43164193-43164212 MS.gene004153:CDS 25.0%
!! ATTCTTCAAACATTTTGAAC+GGG - chr4.2:43163533-43163552 MS.gene004153:intron 25.0%
!! TAGATGAAGTTCATCAATTT+TGG - chr4.2:43162315-43162334 MS.gene004153:intron 25.0%
!! TGTAGAAAGTTTGAAACATT+TGG - chr4.2:43163840-43163859 MS.gene004153:intron 25.0%
!!! AGAAGTATATTTTAAGAAGG+TGG - chr4.2:43163922-43163941 MS.gene004153:intron 25.0%
!!! ATGTAGATTTGTTTGTTGTA+AGG - chr4.2:43162415-43162434 MS.gene004153:CDS 25.0%
!!! CTGAGAAGTATATTTTAAGA+AGG - chr4.2:43163919-43163938 MS.gene004153:intron 25.0%
!!! TAAGGATAGTCACTTTTAAA+AGG + chr4.2:43164465-43164484 None:intergenic 25.0%
AGACAAGTGATTCATTTAAG+TGG - chr4.2:43163071-43163090 MS.gene004153:CDS 30.0%
AGTGTCTCAAGTATATCATA+TGG - chr4.2:43164340-43164359 MS.gene004153:CDS 30.0%
CAAAATGGAATTAAACACCT+TGG - chr4.2:43163232-43163251 MS.gene004153:CDS 30.0%
GAAACATTACTTGAAACTGT+TGG + chr4.2:43164191-43164210 None:intergenic 30.0%
GTGAATATGTCATTACTATG+GGG - chr4.2:43163757-43163776 MS.gene004153:intron 30.0%
TCATCGTAAGTGTAAATGTA+AGG + chr4.2:43164483-43164502 None:intergenic 30.0%
TGAATATGTCATTACTATGG+GGG - chr4.2:43163758-43163777 MS.gene004153:intron 30.0%
TGATATGCATGAAAGAAAGA+TGG + chr4.2:43162854-43162873 None:intergenic 30.0%
TGTCATGCGTAAGAAAATAA+TGG - chr4.2:43164118-43164137 MS.gene004153:CDS 30.0%
TTTCTTTCATGCATATCAGA+TGG - chr4.2:43162856-43162875 MS.gene004153:CDS 30.0%
! ATATTTTCAGCGACAATTGT+TGG - chr4.2:43162823-43162842 MS.gene004153:CDS 30.0%
! ATTTTGGATTACGCGTATTT+TGG - chr4.2:43162926-43162945 MS.gene004153:CDS 30.0%
! CAGTTTCAAGTAATGTTTCT+AGG - chr4.2:43164192-43164211 MS.gene004153:CDS 30.0%
! GTAAGAAAATAATGGAACGT+CGG - chr4.2:43164126-43164145 MS.gene004153:CDS 30.0%
! GTGTGATGCAAATATGATTT+TGG - chr4.2:43162910-43162929 MS.gene004153:CDS 30.0%
!! AGATTTGTTTGTTGTAAGGA+AGG - chr4.2:43162419-43162438 MS.gene004153:CDS 30.0%
!! ATTGATTTGGCTAATGATTC+TGG - chr4.2:43162653-43162672 MS.gene004153:CDS 30.0%
!! GATTTGTTTGTTGTAAGGAA+GGG - chr4.2:43162420-43162439 MS.gene004153:CDS 30.0%
!!! GGTTTTTTCTTGAATATCGT+TGG + chr4.2:43164221-43164240 None:intergenic 30.0%
AAACCAGAAAACAAAGCAAG+AGG + chr4.2:43163001-43163020 None:intergenic 35.0%
ACAAGCACATGAGATTGATT+TGG - chr4.2:43162640-43162659 MS.gene004153:CDS 35.0%
ATCACACTTACACGTTATGA+TGG - chr4.2:43162518-43162537 MS.gene004153:CDS 35.0%
ATCTTGTATGCAACCGAATT+TGG - chr4.2:43163500-43163519 MS.gene004153:intron 35.0%
ATGAAGAAAGCAGTAACACT+TGG - chr4.2:43163432-43163451 MS.gene004153:intron 35.0%
ATGATCTTCATGATAACACC+TGG - chr4.2:43163365-43163384 MS.gene004153:CDS 35.0%
CAAGTATATCATATGGTCGA+TGG - chr4.2:43164347-43164366 MS.gene004153:CDS 35.0%
CTTTCTACAACTACAAGCAA+TGG + chr4.2:43163829-43163848 None:intergenic 35.0%
TAATGTTGGATTTACACGAG+TGG - chr4.2:43162730-43162749 MS.gene004153:CDS 35.0%
TAGCTTAACAGCGTATTTGA+TGG - chr4.2:43164427-43164446 MS.gene004153:CDS 35.0%
TCAAATTTCATTCCAACCCT+TGG + chr4.2:43162290-43162309 None:intergenic 35.0%
TCACACTTACACGTTATGAT+GGG - chr4.2:43162519-43162538 MS.gene004153:CDS 35.0%
TCACCAATACTGATCCAAAT+GGG - chr4.2:43163636-43163655 MS.gene004153:intron 35.0%
TGACATCCGTAAATGTTGAT+TGG - chr4.2:43162261-43162280 MS.gene004153:CDS 35.0%
TGGCTTCCAATCAACATTTA+CGG + chr4.2:43162270-43162289 None:intergenic 35.0%
! AAATGTTGATTGGAAGCCAA+GGG - chr4.2:43162271-43162290 MS.gene004153:CDS 35.0%
! AGACAAAGAAGTATGGTGTA+CGG - chr4.2:43162779-43162798 MS.gene004153:CDS 35.0%
! TAAATGTTGATTGGAAGCCA+AGG - chr4.2:43162270-43162289 MS.gene004153:CDS 35.0%
!! AGCATAAACTGATTTGATCC+AGG + chr4.2:43163386-43163405 None:intergenic 35.0%
!! TTTCTGGTTTCAACCACTAT+CGG - chr4.2:43163011-43163030 MS.gene004153:CDS 35.0%
!!! ATTTTGAGATCCTCTTCACA+AGG + chr4.2:43164080-43164099 None:intergenic 35.0%
!!! GATTCAGACTCTTTTTTCTC+TGG + chr4.2:43163732-43163751 None:intergenic 35.0%
!!! TTTTGGTGATGTTGTGTCAT+TGG - chr4.2:43162943-43162962 MS.gene004153:CDS 35.0%
AACTTATGAGCACACATGCA+GGG - chr4.2:43162699-43162718 MS.gene004153:CDS 40.0%
ACACCTTGGAAACAAGATGA+AGG - chr4.2:43163246-43163265 MS.gene004153:CDS 40.0%
ACTTGAGACACTTGACTACA+TGG + chr4.2:43164332-43164351 None:intergenic 40.0%
ACTTGGCACTTGTGTCAAAA+TGG - chr4.2:43163217-43163236 MS.gene004153:CDS 40.0%
ATGAGCCACGACAAATACAA+GGG - chr4.2:43164396-43164415 MS.gene004153:CDS 40.0%
ATTTGTCGTGGCTCATAAAC+TGG + chr4.2:43164392-43164411 None:intergenic 40.0%
CACAAAAGCGACATAAGAGT+TGG - chr4.2:43164258-43164277 MS.gene004153:CDS 40.0%
CACCAATACTGATCCAAATG+GGG - chr4.2:43163637-43163656 MS.gene004153:intron 40.0%
CACCTTGGAAACAAGATGAA+GGG - chr4.2:43163247-43163266 MS.gene004153:CDS 40.0%
GTCACCAATACTGATCCAAA+TGG - chr4.2:43163635-43163654 MS.gene004153:intron 40.0%
GTGTGTATACTTTCCCCATT+TGG + chr4.2:43163653-43163672 None:intergenic 40.0%
TAACCTTGCAAGCTTATGTG+AGG + chr4.2:43163604-43163623 None:intergenic 40.0%
TATGAGCCACGACAAATACA+AGG - chr4.2:43164395-43164414 MS.gene004153:CDS 40.0%
TCTACAACTACAAGCAATGG+AGG + chr4.2:43163826-43163845 None:intergenic 40.0%
TGGGGGAACTTCCAAAATAT+TGG - chr4.2:43163775-43163794 MS.gene004153:intron 40.0%
TTCCCCATTTGGATCAGTAT+TGG + chr4.2:43163642-43163661 None:intergenic 40.0%
! CTTCGTGTTCTAGACAACTT+TGG + chr4.2:43164008-43164027 None:intergenic 40.0%
! TACACGTTATGATGGGAAGT+TGG - chr4.2:43162526-43162545 MS.gene004153:CDS 40.0%
! TGTGATGCTCTTTTTGTGCT+AGG + chr4.2:43164242-43164261 None:intergenic 40.0%
! TTTCATAAAAGGTGCAGCTG+AGG - chr4.2:43164049-43164068 MS.gene004153:CDS 40.0%
!! TTCTGGTTTCAACCACTATC+GGG - chr4.2:43163012-43163031 MS.gene004153:CDS 40.0%
!!! AGACCTCTTGCTTTGTTTTC+TGG - chr4.2:43162995-43163014 MS.gene004153:CDS 40.0%
!!! CTAGGTGGAGCACTTTTTTT+CGG + chr4.2:43164302-43164321 None:intergenic 40.0%
AAGCAAGAGGTCTATTCGCA+TGG + chr4.2:43162988-43163007 None:intergenic 45.0%
AGTCCTCACATAAGCTTGCA+AGG - chr4.2:43163598-43163617 MS.gene004153:intron 45.0%
AGTGCCAAGTGCATAAACCA+TGG + chr4.2:43163207-43163226 None:intergenic 45.0%
CAACTTATGAGCACACATGC+AGG - chr4.2:43162698-43162717 MS.gene004153:CDS 45.0%
CATAAACCATGGTGAGTCTC+GGG + chr4.2:43163196-43163215 None:intergenic 45.0%
CTCACCATGGTTTATGCACT+TGG - chr4.2:43163200-43163219 MS.gene004153:CDS 45.0%
CTTGGAAACAAGATGAAGGG+AGG - chr4.2:43163250-43163269 MS.gene004153:CDS 45.0%
GACCCAAGCTGCTTCAAAAT+CGG + chr4.2:43163329-43163348 None:intergenic 45.0%
GCAGGGCATAGATCTAATGT+TGG - chr4.2:43162716-43162735 MS.gene004153:CDS 45.0%
GCATAAACCATGGTGAGTCT+CGG + chr4.2:43163197-43163216 None:intergenic 45.0%
GTCATGAATGCCGTTGTCTT+TGG + chr4.2:43164159-43164178 None:intergenic 45.0%
TGACTACATGGTTGCTCTCT+AGG + chr4.2:43164320-43164339 None:intergenic 45.0%
TGAGTCCCTTGTATTTGTCG+TGG + chr4.2:43164404-43164423 None:intergenic 45.0%
TGATCGGTGAAGACAGTCTT+TGG + chr4.2:43163136-43163155 None:intergenic 45.0%
TGGCGTTGTGCAAGATGTTA+GGG - chr4.2:43163962-43163981 MS.gene004153:intron 45.0%
TTTCAACCACTATCGGGGTT+CGG - chr4.2:43163018-43163037 MS.gene004153:CDS 45.0%
! CTCCCTTCATCTTGTTTCCA+AGG + chr4.2:43163252-43163271 None:intergenic 45.0%
! GGTCGATGGAGTATCTTGTT+CGG - chr4.2:43164361-43164380 MS.gene004153:CDS 45.0%
! TCTGGTTTCAACCACTATCG+GGG - chr4.2:43163013-43163032 MS.gene004153:CDS 45.0%
!! TATCGGGGTTCGGTCATTTT+TGG - chr4.2:43163028-43163047 MS.gene004153:CDS 45.0%
!!! AAGCCGATTTTGAAGCAGCT+TGG - chr4.2:43163323-43163342 MS.gene004153:CDS 45.0%
!!! AGCCGATTTTGAAGCAGCTT+GGG - chr4.2:43163324-43163343 MS.gene004153:CDS 45.0%
AAATGACCGAACCCCGATAG+TGG + chr4.2:43163027-43163046 None:intergenic 50.0%
ATCTTGCACAACGCCACTTC+GGG + chr4.2:43163958-43163977 None:intergenic 50.0%
CACCGATCAAGATCCAGCAA+TGG - chr4.2:43163147-43163166 MS.gene004153:CDS 50.0%
CATCACTTCAGCAAGTGCCT+TGG + chr4.2:43163173-43163192 None:intergenic 50.0%
CATCTTGCACAACGCCACTT+CGG + chr4.2:43163959-43163978 None:intergenic 50.0%
CGGTCAAACGCCAAAGACAA+CGG - chr4.2:43164146-43164165 MS.gene004153:CDS 50.0%
CTACATGGTTGCTCTCTAGG+TGG + chr4.2:43164317-43164336 None:intergenic 50.0%
CTTGCTTATGCTGCACGTAC+TGG - chr4.2:43162338-43162357 MS.gene004153:intron 50.0%
GCAAGATGTTAGGGGGAAGA+CGG - chr4.2:43163971-43163990 MS.gene004153:intron 50.0%
GCAAGCATGTGTGTTGTCTC+TGG + chr4.2:43162596-43162615 None:intergenic 50.0%
GCAAGGTCTGTCTCACTAAG+TGG + chr4.2:43164545-43164564 None:intergenic 50.0%
GCGTTGTGCAAGATGTTAGG+GGG - chr4.2:43163964-43163983 MS.gene004153:intron 50.0%
GGCCATTGCTGGATCTTGAT+CGG + chr4.2:43163152-43163171 None:intergenic 50.0%
GGCGTTGTGCAAGATGTTAG+GGG - chr4.2:43163963-43163982 MS.gene004153:intron 50.0%
GTGGCGTTGTGCAAGATGTT+AGG - chr4.2:43163961-43163980 MS.gene004153:intron 50.0%
GTTGATTGGAAGCCAAGGGT+TGG - chr4.2:43162275-43162294 MS.gene004153:CDS 50.0%
TCAAGATCCAGCAATGGCCA+AGG - chr4.2:43163153-43163172 MS.gene004153:CDS 50.0%
TGTCGTGGCTCATAAACTGG+AGG + chr4.2:43164389-43164408 None:intergenic 50.0%
! TCTCTGGAGATAGCAGCTCA+TGG + chr4.2:43162580-43162599 None:intergenic 50.0%
! TGAGGCATCACCTTGTGAAG+AGG - chr4.2:43164067-43164086 MS.gene004153:CDS 50.0%
!! TATGCTGCACGTACTGGCTT+TGG - chr4.2:43162344-43162363 MS.gene004153:intron 50.0%
AGTATGGTGTACGGCGAAGC+TGG - chr4.2:43162788-43162807 MS.gene004153:CDS 55.0%
TGGACAAGAGATGCCCGAAG+TGG - chr4.2:43163942-43163961 MS.gene004153:intron 55.0%
GCAAGTGCCTTGGCCATTGC+TGG + chr4.2:43163163-43163182 None:intergenic 60.0%
GTGATGCCCGAGACTCACCA+TGG - chr4.2:43163187-43163206 MS.gene004153:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 43162260 43164577 43162260 ID=MS.gene004153
chr4.2 mRNA 43162260 43164577 43162260 ID=MS.gene004153.t1;Parent=MS.gene004153
chr4.2 exon 43164044 43164577 43164044 ID=MS.gene004153.t1.exon1;Parent=MS.gene004153.t1
chr4.2 CDS 43164044 43164577 43164044 ID=cds.MS.gene004153.t1;Parent=MS.gene004153.t1
chr4.2 exon 43162389 43163399 43162389 ID=MS.gene004153.t1.exon2;Parent=MS.gene004153.t1
chr4.2 CDS 43162389 43163399 43162389 ID=cds.MS.gene004153.t1;Parent=MS.gene004153.t1
chr4.2 exon 43162260 43162298 43162260 ID=MS.gene004153.t1.exon3;Parent=MS.gene004153.t1
chr4.2 CDS 43162260 43162298 43162260 ID=cds.MS.gene004153.t1;Parent=MS.gene004153.t1
Gene Sequence

>MS.gene004153

ATGACATCCGTAAATGTTGATTGGAAGCCAAGGGTTGGAATGAAATTTGACAACATAGATGAAGTTCATCAATTTTGGCTTGCTTATGCTGCACGTACTGGCTTTGGAATTAGAACACGCTATGCGAACAAAAATAAAGACGGCATTGTATCATCATGTAGATTTGTTTGTTGTAAGGAAGGGCTGCGAAAGATAAATGAAGCAAATGCTTATGTTAGCAAACACAGAAAAGAGACTAGAACAGATTGCAATGTAAAGATCACACTTACACGTTATGATGGGAAGTTGGTCATCCATGAATTTGAAGATAAGCATAACCATGAGCTGCTATCTCCAGAGACAACACACATGCTTGCATCACATAGAAAGATAACTGAAGTACAAGCACATGAGATTGATTTGGCTAATGATTCTGGATTAATGCAAAAGTCAACATTTCAACTTATGAGCACACATGCAGGGCATAGATCTAATGTTGGATTTACACGAGTGGATGTAAAAAATTACATTAGTAAAAGAAGACAAAGAAGTATGGTGTACGGCGAAGCTGGATGTATTTCACAATATTTTCAGCGACAATTGTTGGAGAATCCATCTTTCTTTCATGCATATCAGATGGATATAGAAGAACAAATGACAAATGTGTTTTGGTGTGATGCAAATATGATTTTGGATTACGCGTATTTTGGTGATGTTGTGTCATTGGACACTACATATTGTACTAACCATGCGAATAGACCTCTTGCTTTGTTTTCTGGTTTCAACCACTATCGGGGTTCGGTCATTTTTGGTGCAGCACTACTGTATGATGAGACAAGTGATTCATTTAAGTGGTTGTTTGATACATTCTTACAAGCACACGACAACAAAAAACCAAAGACTGTCTTCACCGATCAAGATCCAGCAATGGCCAAGGCACTTGCTGAAGTGATGCCCGAGACTCACCATGGTTTATGCACTTGGCACTTGTGTCAAAATGGAATTAAACACCTTGGAAACAAGATGAAGGGAGGAAGTTATTTTTTAAGAGATTTCAAGATGTGCATGTATGATATTGACATTGAAGCCGATTTTGAAGCAGCTTGGGTCAAATTAGTCAATGAATATGATCTTCATGATAACACCTGGATCAAATCAGTTTATGCTATTAAAAAAAATGGGCTTCATGTTATATGAAGAAAGCAGTAACACTTGGTATGCGAAGTACTCAAATCAGTGAAAGTTTGAATGCTCACTTCAAATCTTGTATGCAACCGAATTTGGAAATCATGCAATTCTTCAAACATTTTGAACGGGTTGTTGAAGAGAAGAGAGAGAATGAATTGAGTTGTGTGTATGAGTCCTCACATAAGCTTGCAAGGTTAGTATATGAGAAGTCACCAATACTGATCCAAATGGGGAAAGTATACACACACACTGTATTTGAGTTATTTCAAAACGAGTTTAACTCGTTCTTAGCTTTATCGATGCCAGAGAAAAAAGAGTCTGAATCTTTATGTGAATATGTCATTACTATGGGGGAACTTCCAAAATATTGGATAGTGTTATTTGATCGTGCTTCAACCTCCATTGCTTGTAGTTGTAGAAAGTTTGAAACATTTGGCATACTTTGTTCACATGCATTGAAAGTGATTGAAGCAAATGATGTTAAAGCCATTCCTGAGAAGTATATTTTAAGAAGGTGGACAAGAGATGCCCGAAGTGGCGTTGTGCAAGATGTTAGGGGGAAGACGGTTGAAGCAGATCCAAAGTTGTCTAGAACACGAAGATATAGACAAGTTGTCTCAAATTTCATAAAAGGTGCAGCTGAGGCATCACCTTGTGAAGAGGATCTCAAAATTGTTGAAGATTGTGTAGATGTCATGCGTAAGAAAATAATGGAACGTCGGTCAAACGCCAAAGACAACGGCATTCATGACAATGATAACCCAACAGTTTCAAGTAATGTTTCTAGGGAACCAACGATATTCAAGAAAAAACCTAGCACAAAAAGAGCATCACAAAAGCGACATAAGAGTTGGATTGAGCAACAATCTGCTCCGAAAAAAAGTGCTCCACCTAGAGAGCAACCATGTAGTCAAGTGTCTCAAGTATATCATATGGTCGATGGAGTATCTTGTTCGGCACCTCCAGTTTATGAGCCACGACAAATACAAGGGACTCAATTTAGCTTAACAGCGTATTTGATGGTAATTTATTCATCCTTTTAAAAGTGACTATCCTTACATTTACACTTACGATGATATTAATTAGTCTTTTTTTAAAAAAAAATTATGCAGGCACCACTTAGTGAGACAGACCTTGCAGCATTACACTGA
Protein sequence

>MS.gene004153.t1

MTSVNVDWKPRVGMKFDNIDEVHQFWLAYAARTGFGIRTRYANKNKDGIVSSCRFVCCKEGLRKINEANAYVSKHRKETRTDCNVKITLTRYDGKLVIHEFEDKHNHELLSPETTHMLASHRKITEVQAHEIDLANDSGLMQKSTFQLMSTHAGHRSNVGFTRVDVKNYISKRRQRSMKAVTLGMRSTQISESLNAHFKSCMQPNLEIMQFFKHFERVVEEKRENELSCVYESSHKLARLVYEKSPILIQMGKVYTHTVFELFQNEFNSFLALSMPEKKESESLCEYVITMGELPKYWIVLFDRASTSIACSCRKFETFGILCSHALKVIEANDVKAIPEKYILRRWTRDARSGVVQDVRGKTVEADPKLSRTRRYRQVVSNFIKGAAEASPCEEDLKIVEDCVDVMRKKIMERRSNAKDNGIHDNDNPTVSSNVSREPTIFKKKPSTKRASQKRHKSWIEQQSAPKKSAPPREQPCSQVSQVYHMVDGVSCSAPPVYEPRQIQGTQFSLTAYLMAPLSETDLAALH