AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004158

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004158.t1 MTR_4g060950 92.147 191 8 2 1 185 1 190 3.12e-129 360
MS.gene004158.t1 MTR_2g093310 76.571 175 39 2 11 185 26 198 1.12e-98 283
MS.gene004158.t1 MTR_1g095850 72.024 168 35 6 19 185 73 229 2.13e-77 231
MS.gene004158.t1 MTR_2g068760 71.429 112 31 1 15 126 10 120 2.18e-54 170
MS.gene004158.t1 MTR_3g071590 56.000 150 57 3 17 164 7 149 4.52e-50 159
MS.gene004158.t1 MTR_4g083680 66.364 110 36 1 17 126 14 122 2.20e-49 158
MS.gene004158.t1 MTR_5g083230 64.035 114 40 1 17 130 5 117 2.57e-49 157
MS.gene004158.t1 MTR_3g031660 63.636 110 39 1 17 126 6 114 2.41e-47 153
MS.gene004158.t1 MTR_5g083960 56.140 114 49 1 17 130 38 150 7.97e-42 139
MS.gene004158.t1 MTR_3g094690 55.752 113 49 1 15 127 44 155 4.24e-40 136
MS.gene004158.t1 MTR_6g005070 49.180 122 61 1 11 132 2 122 9.73e-40 132
MS.gene004158.t1 MTR_7g033800 54.955 111 49 1 10 120 3 112 1.56e-39 132
MS.gene004158.t1 MTR_3g077240 55.455 110 48 1 17 126 9 117 2.06e-39 133
MS.gene004158.t1 MTR_4g105170 56.481 108 46 1 17 124 10 116 5.26e-39 132
MS.gene004158.t1 MTR_3g073690 56.311 103 44 1 17 119 8 109 1.56e-38 131
MS.gene004158.t1 MTR_6g005080 42.282 149 72 2 11 159 3 137 1.81e-38 129
MS.gene004158.t1 MTR_3g094690 54.867 113 49 2 15 127 44 154 1.57e-37 129
MS.gene004158.t1 MTR_5g075020 57.522 113 47 1 15 127 40 151 2.14e-37 129
MS.gene004158.t1 MTR_5g075020 57.522 113 47 1 15 127 44 155 3.57e-37 129
MS.gene004158.t1 MTR_5g017950 53.153 111 51 1 17 127 7 116 4.96e-36 128
MS.gene004158.t1 MTR_7g028905 47.321 112 58 1 14 125 1 111 7.61e-35 119
MS.gene004158.t1 MTR_3g071420 57.732 97 40 1 17 113 13 108 3.21e-34 119
MS.gene004158.t1 MTR_3g452660 50.926 108 52 1 17 124 6 112 8.83e-34 121
MS.gene004158.t1 MTR_5g083010 56.701 97 41 1 17 113 10 105 2.35e-32 114
MS.gene004158.t1 MTR_6g027710 44.118 136 66 4 15 148 37 164 5.33e-32 115
MS.gene004158.t1 MTR_7g096530 52.885 104 47 2 17 119 13 115 5.77e-32 115
MS.gene004158.t1 MTR_8g040900 60.976 82 31 1 17 98 6 86 9.05e-32 114
MS.gene004158.t1 MTR_5g093010 41.964 112 64 1 14 125 1 111 3.99e-31 110
MS.gene004158.t1 MTR_7g075230 41.935 155 72 4 17 168 11 150 3.34e-30 109
MS.gene004158.t1 MTR_1g070205 40.854 164 80 6 18 175 12 164 3.42e-30 110
MS.gene004158.t1 MTR_1g070220 48.214 112 55 3 18 127 15 125 9.60e-30 108
MS.gene004158.t1 MTR_8g079660 46.667 105 55 1 15 119 5 108 1.10e-29 110
MS.gene004158.t1 MTR_7g074990 49.515 103 50 2 18 119 20 121 2.16e-29 108
MS.gene004158.t1 MTR_8g036105 49.074 108 53 2 17 123 17 123 1.77e-28 105
MS.gene004158.t1 MTR_8g036085 46.789 109 56 2 17 124 22 129 2.29e-28 105
MS.gene004158.t1 MTR_8g079620 45.714 105 56 1 15 119 5 108 1.76e-27 103
MS.gene004158.t1 MTR_5g080470 45.378 119 56 3 17 127 6 123 6.32e-27 101
MS.gene004158.t1 MTR_7g096610 52.041 98 45 2 17 113 7 103 1.13e-26 101
MS.gene004158.t1 MTR_8g079580 43.396 106 60 0 15 120 5 110 1.61e-26 101
MS.gene004158.t1 MTR_6g027700 43.137 102 56 2 18 118 7 107 6.09e-26 99.0
MS.gene004158.t1 MTR_6g011250 35.714 168 89 6 19 181 10 163 3.97e-25 96.3
MS.gene004158.t1 MTR_6g011200 42.149 121 58 4 19 131 10 126 7.96e-23 92.0
MS.gene004158.t1 MTR_4g107450 43.617 94 52 1 19 112 11 103 1.55e-22 92.0
MS.gene004158.t1 MTR_6g011230 42.553 94 53 1 19 112 12 104 9.64e-21 85.5
MS.gene004158.t1 MTR_4g099230 44.211 95 47 3 19 108 3 96 9.93e-20 81.6
MS.gene004158.t1 MTR_2g100020 37.624 101 62 1 19 119 20 119 1.52e-19 83.6
MS.gene004158.t1 MTR_8g017090 41.489 94 54 1 19 112 13 105 1.14e-18 81.6
MS.gene004158.t1 MTR_5g085390 45.679 81 42 2 19 98 10 89 7.02e-16 73.2
MS.gene004158.t1 MTR_4g088035 34.483 87 56 1 19 105 7 92 5.66e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004158.t1 AT2G28500 66.129 186 45 6 14 185 51 232 9.87e-76 227
MS.gene004158.t1 AT1G07900 62.500 176 47 4 14 185 29 189 2.22e-72 217
MS.gene004158.t1 AT2G30130 67.890 109 34 1 17 125 7 114 7.68e-50 160
MS.gene004158.t1 AT1G16530 58.716 109 45 0 15 123 11 119 6.05e-46 149
MS.gene004158.t1 AT1G31320 68.468 111 34 1 17 127 12 121 5.61e-44 144
MS.gene004158.t1 AT5G63090 57.407 108 45 1 17 124 10 116 3.58e-40 135
MS.gene004158.t1 AT5G63090 57.407 108 45 1 17 124 10 116 3.58e-40 135
MS.gene004158.t1 AT5G63090 57.407 108 45 1 17 124 10 116 3.58e-40 135
MS.gene004158.t1 AT5G63090 57.407 108 45 1 17 124 10 116 3.58e-40 135
MS.gene004158.t1 AT3G27650 58.654 104 42 1 17 120 38 140 3.64e-40 134
MS.gene004158.t1 AT2G30340 50.862 116 56 1 15 130 49 163 6.82e-40 136
MS.gene004158.t1 AT2G30340 50.862 116 56 1 15 130 50 164 7.30e-40 136
MS.gene004158.t1 AT2G23660 55.238 105 46 1 17 121 4 107 9.37e-39 135
MS.gene004158.t1 AT2G23660 55.238 105 46 1 17 121 4 107 9.37e-39 135
MS.gene004158.t1 AT2G23660 55.238 105 46 1 17 121 4 107 9.37e-39 135
MS.gene004158.t1 AT3G26620 54.237 118 49 3 16 129 1 117 1.01e-38 129
MS.gene004158.t1 AT3G26660 56.604 106 43 2 16 119 1 105 2.61e-38 128
MS.gene004158.t1 AT5G66870 51.128 133 62 2 17 149 6 135 4.40e-37 130
MS.gene004158.t1 AT2G40470 57.143 112 47 1 16 127 18 128 2.01e-36 125
MS.gene004158.t1 AT2G40470 56.637 113 48 1 15 127 42 153 3.63e-36 125
MS.gene004158.t1 AT3G11090 51.923 104 49 1 17 120 10 112 3.46e-33 116
MS.gene004158.t1 AT4G00210 52.778 108 49 2 17 123 10 116 6.52e-33 117
MS.gene004158.t1 AT1G65620 60.227 88 34 1 11 98 2 88 1.93e-32 115
MS.gene004158.t1 AT1G65620 60.227 88 34 1 11 98 2 88 1.93e-32 115
MS.gene004158.t1 AT1G65620 60.227 88 34 1 11 98 2 88 1.93e-32 115
MS.gene004158.t1 AT1G65620 60.227 88 34 1 11 98 2 88 1.93e-32 115
MS.gene004158.t1 AT1G65620 60.227 88 34 1 11 98 2 88 1.93e-32 115
MS.gene004158.t1 AT3G03760 47.788 113 57 2 17 128 50 161 1.62e-31 115
MS.gene004158.t1 AT2G42430 46.154 117 61 2 13 128 10 125 1.84e-29 109
MS.gene004158.t1 AT4G00220 49.524 105 51 2 21 124 20 123 3.64e-29 108
MS.gene004158.t1 AT2G45410 42.105 152 69 4 21 171 19 152 5.52e-29 106
MS.gene004158.t1 AT2G45420 47.619 105 53 2 21 124 40 143 4.46e-27 103
MS.gene004158.t1 AT2G42440 53.608 97 43 2 17 112 6 101 9.88e-27 102
MS.gene004158.t1 AT2G31310 47.573 103 52 2 17 118 6 107 1.13e-26 100
MS.gene004158.t1 AT3G58190 38.125 160 83 5 18 176 11 155 1.57e-26 100
MS.gene004158.t1 AT5G06080 43.363 113 62 2 17 128 6 117 1.17e-25 97.4
MS.gene004158.t1 AT3G47870 44.860 107 53 2 19 125 37 137 6.67e-25 99.0
MS.gene004158.t1 AT3G50510 41.441 111 64 1 9 119 3 112 2.93e-24 94.4
MS.gene004158.t1 AT3G50510 41.441 111 64 1 9 119 3 112 2.93e-24 94.4
MS.gene004158.t1 AT1G72980 36.232 138 77 3 8 145 5 131 1.87e-22 90.1
MS.gene004158.t1 AT5G35900 39.604 101 60 1 19 119 6 105 4.27e-22 89.0
MS.gene004158.t1 AT3G13850 35.256 156 84 5 19 169 37 180 2.07e-21 88.6
MS.gene004158.t1 AT1G06280 38.824 85 48 2 19 100 25 108 1.86e-15 71.6
MS.gene004158.t1 AT4G22700 37.864 103 59 4 19 119 6 105 2.81e-15 70.9
MS.gene004158.t1 AT2G19820 37.634 93 56 2 14 105 8 99 9.58e-14 65.1

Find 35 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCAGCTCTTTGAGCTTC+TGG 0.203584 4.2:-43227049 None:intergenic
GAAGCTCATTGACTTGCTTT+TGG 0.237617 4.2:-43227150 None:intergenic
AGAGTTAGAGACCCAGTTTA+TGG 0.300502 4.2:+43227103 MS.gene004158:CDS
GAGCAACAAGATTGGCTTGC+TGG 0.325135 4.2:-43227219 None:intergenic
GCTCCTGCACAACCATAAAC+TGG 0.336614 4.2:-43227115 None:intergenic
CAACATCATCAAATTCTTAC+AGG 0.339119 4.2:+43225706 MS.gene004158:CDS
TACAACCTCAGCTTGTGATT+TGG 0.347506 4.2:-43227185 None:intergenic
TGCTGCTGATAAATGTGTTT+TGG 0.380702 4.2:+43225619 MS.gene004158:CDS
CATAGCAATGGACATCATAT+CGG 0.414964 4.2:+43227322 MS.gene004158:CDS
GGATCAGTAGGAGGAAAATA+AGG 0.417860 4.2:-43225644 None:intergenic
GCAAAATAGAGCAACAAGAT+TGG 0.441636 4.2:-43227227 None:intergenic
CGATATGATGTCCATTGCTA+TGG 0.449199 4.2:-43227321 None:intergenic
GACCCAGTTTATGGTTGTGC+AGG 0.466543 4.2:+43227112 MS.gene004158:CDS
TCTATGAGCAATGGTAAACT+TGG 0.485039 4.2:-43225669 None:intergenic
AGTGACAAGCATGGTTTATG+AGG 0.515235 4.2:+43227072 MS.gene004158:CDS
TGAGCAATGGTAAACTTGGC+AGG 0.527769 4.2:-43225665 None:intergenic
GCTCAGGGTGGGATACACTT+TGG 0.537894 4.2:+43227377 MS.gene004158:CDS
GACAACACTAGCCTATGCTC+AGG 0.542861 4.2:+43227361 MS.gene004158:CDS
CTCCTGCACAACCATAAACT+GGG 0.571360 4.2:-43227114 None:intergenic
GTAAACTTGGCAGGATCAGT+AGG 0.577739 4.2:-43225656 None:intergenic
AAAATCTTGCAAGCAGCACA+AGG 0.584368 4.2:-43225584 None:intergenic
TGATGTCCATTGCTATGGGA+AGG 0.587814 4.2:-43227316 None:intergenic
AACTGTTGTGGTGATTCCTG+TGG 0.589765 4.2:-43227268 None:intergenic
GTGTATCCCACCCTGAGCAT+AGG 0.591383 4.2:-43227372 None:intergenic
GATATGATGTCCATTGCTAT+GGG 0.597738 4.2:-43227320 None:intergenic
CACTAGCCTATGCTCAGGGT+GGG 0.599833 4.2:+43227366 MS.gene004158:CDS
GGAAATGTCACAAAGTCCAC+AGG 0.603176 4.2:+43227252 MS.gene004158:CDS
CAAAGTCCTTCCCATAGCAA+TGG 0.614127 4.2:+43227310 MS.gene004158:CDS
AGCTGATGCAGTGACAAGCA+TGG 0.615883 4.2:+43227063 MS.gene004158:CDS
AACTTGGCAGGATCAGTAGG+AGG 0.640755 4.2:-43225653 None:intergenic
ATCACCACAACAGTTATCAG+TGG 0.656563 4.2:+43227276 MS.gene004158:CDS
ACTAGCCAAATCACAAGCTG+AGG 0.662044 4.2:+43227180 MS.gene004158:CDS
ACAACACTAGCCTATGCTCA+GGG 0.673423 4.2:+43227362 MS.gene004158:CDS
TCATCCACTGATAACTGTTG+TGG 0.694554 4.2:-43227280 None:intergenic
ACACTAGCCTATGCTCAGGG+TGG 0.697314 4.2:+43227365 MS.gene004158:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATGGAATAATAATATAT+AGG + chr4.2:43226175-43226194 MS.gene004158:intron 15.0%
!! GAAACAAAACAAAAAAATTT+AGG + chr4.2:43226450-43226469 MS.gene004158:intron 15.0%
!!! CAATTTTAATATTGATTTGT+AGG + chr4.2:43226107-43226126 MS.gene004158:intron 15.0%
!!! TACTATTTGTTAGAATTATT+AGG - chr4.2:43226624-43226643 None:intergenic 15.0%
!!! TTTTTGTTGTTGAAAAAAAT+AGG + chr4.2:43227021-43227040 MS.gene004158:intron 15.0%
!! AAAAATAGAAGCTAAAGAAT+AGG + chr4.2:43226707-43226726 MS.gene004158:intron 20.0%
!! ACTAGAAAATGTTGAAATAT+TGG + chr4.2:43225740-43225759 MS.gene004158:intron 20.0%
!! TATACATTCAACATTATCAA+AGG + chr4.2:43226368-43226387 MS.gene004158:intron 20.0%
!!! ATTCTTTAGCTTCTATTTTT+AGG - chr4.2:43226707-43226726 None:intergenic 20.0%
!!! ATTTATTTGAGGCATTATAA+CGG - chr4.2:43226588-43226607 None:intergenic 20.0%
!!! ATTTTTCTTGAAAAACCTAA+AGG + chr4.2:43226920-43226939 MS.gene004158:intron 20.0%
!!! TATTTGTTAGAATTATTAGG+TGG - chr4.2:43226621-43226640 None:intergenic 20.0%
!!! TTTTTTTTCCTTAAACCTTT+AGG - chr4.2:43226938-43226957 None:intergenic 20.0%
! TTCTAACAAATAGTACCTAA+TGG + chr4.2:43226629-43226648 MS.gene004158:intron 25.0%
! TTGAAAAACCTAAAGGTTTA+AGG + chr4.2:43226927-43226946 MS.gene004158:intron 25.0%
! TTTGAATCAATGATGAAAGA+TGG + chr4.2:43226159-43226178 MS.gene004158:intron 25.0%
!!! GTATCAAGATTTACTTTTTG+AGG + chr4.2:43226334-43226353 MS.gene004158:intron 25.0%
!!! TCTATTTTTAGGTCAAGATT+TGG - chr4.2:43226696-43226715 None:intergenic 25.0%
AACAAATAGTACCTAATGGT+GGG + chr4.2:43226633-43226652 MS.gene004158:intron 30.0%
AAGTGTTATGTATGACTACA+AGG - chr4.2:43226666-43226685 None:intergenic 30.0%
AATAGAAGCTAAAGAATAGG+AGG + chr4.2:43226710-43226729 MS.gene004158:intron 30.0%
ATTGTTGTGATCTAAGTAAG+TGG + chr4.2:43226257-43226276 MS.gene004158:intron 30.0%
CAACATCATCAAATTCTTAC+AGG + chr4.2:43225706-43225725 MS.gene004158:CDS 30.0%
CCATGGACCATAATAAAAAA+GGG - chr4.2:43226545-43226564 None:intergenic 30.0%
GTTGACAATGCATTTATTTG+AGG - chr4.2:43226599-43226618 None:intergenic 30.0%
TAACAAATAGTACCTAATGG+TGG + chr4.2:43226632-43226651 MS.gene004158:intron 30.0%
TCCATGGACCATAATAAAAA+AGG - chr4.2:43226546-43226565 None:intergenic 30.0%
TTTAGGAGTGAAACATGATA+AGG + chr4.2:43226467-43226486 MS.gene004158:intron 30.0%
TTTATTATGGTCCATGGATA+TGG + chr4.2:43226548-43226567 MS.gene004158:intron 30.0%
AAAACCAAGTGTAAACGAAG+TGG + chr4.2:43226783-43226802 MS.gene004158:intron 35.0%
ACCAAAAACTCTATGAGCAA+TGG - chr4.2:43225681-43225700 None:intergenic 35.0%
CATAGCAATGGACATCATAT+CGG + chr4.2:43227322-43227341 MS.gene004158:CDS 35.0%
GATATGATGTCCATTGCTAT+GGG - chr4.2:43227323-43227342 None:intergenic 35.0%
GCAAAATAGAGCAACAAGAT+TGG - chr4.2:43227230-43227249 None:intergenic 35.0%
TCTATGAGCAATGGTAAACT+TGG - chr4.2:43225672-43225691 None:intergenic 35.0%
TGAGGTTCCAAATCATGTAA+AGG + chr4.2:43226514-43226533 MS.gene004158:intron 35.0%
TGATCTAAGTAAGTGGTAAG+AGG + chr4.2:43226264-43226283 MS.gene004158:intron 35.0%
! ACATGATAAGGAAGCATACA+TGG + chr4.2:43226479-43226498 MS.gene004158:intron 35.0%
! ACATGGTTGAGATTTTGTTG+AGG + chr4.2:43226496-43226515 MS.gene004158:intron 35.0%
! ACCATTGCTCATAGAGTTTT+TGG + chr4.2:43225677-43225696 MS.gene004158:CDS 35.0%
! AGGCATTATAACGGAAAGTA+CGG - chr4.2:43226579-43226598 None:intergenic 35.0%
! TCTTGTTGCTCTATTTTGCA+TGG + chr4.2:43227231-43227250 MS.gene004158:CDS 35.0%
! TGCTGCTGATAAATGTGTTT+TGG + chr4.2:43225619-43225638 MS.gene004158:CDS 35.0%
!!! CCCTTTTTTATTATGGTCCA+TGG + chr4.2:43226542-43226561 MS.gene004158:intron 35.0%
AAAATCTTGCAAGCAGCACA+AGG - chr4.2:43225587-43225606 None:intergenic 40.0%
AGAGTTAGAGACCCAGTTTA+TGG + chr4.2:43227103-43227122 MS.gene004158:CDS 40.0%
AGTGACAAGCATGGTTTATG+AGG + chr4.2:43227072-43227091 MS.gene004158:CDS 40.0%
ATCACCACAACAGTTATCAG+TGG + chr4.2:43227276-43227295 MS.gene004158:CDS 40.0%
ATGTATGACTACAAGGAGAG+AGG - chr4.2:43226659-43226678 None:intergenic 40.0%
CGATATGATGTCCATTGCTA+TGG - chr4.2:43227324-43227343 None:intergenic 40.0%
GGACCATAATAAAAAAGGGC+AGG - chr4.2:43226541-43226560 None:intergenic 40.0%
GGATCAGTAGGAGGAAAATA+AGG - chr4.2:43225647-43225666 None:intergenic 40.0%
TACAACCTCAGCTTGTGATT+TGG - chr4.2:43227188-43227207 None:intergenic 40.0%
TCATCCACTGATAACTGTTG+TGG - chr4.2:43227283-43227302 None:intergenic 40.0%
TTAGCCACTTCGTTTACACT+TGG - chr4.2:43226790-43226809 None:intergenic 40.0%
! CATTATAACGGAAAGTACGG+GGG - chr4.2:43226576-43226595 None:intergenic 40.0%
! GCATTATAACGGAAAGTACG+GGG - chr4.2:43226577-43226596 None:intergenic 40.0%
! GGCATTATAACGGAAAGTAC+GGG - chr4.2:43226578-43226597 None:intergenic 40.0%
!! CTTGATAGCGACTGTTGATT+AGG + chr4.2:43226857-43226876 MS.gene004158:intron 40.0%
!!! GAAGCTCATTGACTTGCTTT+TGG - chr4.2:43227153-43227172 None:intergenic 40.0%
!!! GGTCCTGCCCTTTTTTATTA+TGG + chr4.2:43226535-43226554 MS.gene004158:intron 40.0%
AACTGTTGTGGTGATTCCTG+TGG - chr4.2:43227271-43227290 None:intergenic 45.0%
ACTAGCCAAATCACAAGCTG+AGG + chr4.2:43227180-43227199 MS.gene004158:CDS 45.0%
CAAAGTCCTTCCCATAGCAA+TGG + chr4.2:43227310-43227329 MS.gene004158:CDS 45.0%
CTCCTGCACAACCATAAACT+GGG - chr4.2:43227117-43227136 None:intergenic 45.0%
GGAAATGTCACAAAGTCCAC+AGG + chr4.2:43227252-43227271 MS.gene004158:CDS 45.0%
GGCAGGACCTTTACATGATT+TGG - chr4.2:43226524-43226543 None:intergenic 45.0%
GTAAACTTGGCAGGATCAGT+AGG - chr4.2:43225659-43225678 None:intergenic 45.0%
TGAGCAATGGTAAACTTGGC+AGG - chr4.2:43225668-43225687 None:intergenic 45.0%
TGATGTCCATTGCTATGGGA+AGG - chr4.2:43227319-43227338 None:intergenic 45.0%
! ACAACACTAGCCTATGCTCA+GGG + chr4.2:43227362-43227381 MS.gene004158:CDS 45.0%
!!! GTGCTGCTTGCAAGATTTTG+AGG + chr4.2:43225588-43225607 MS.gene004158:CDS 45.0%
AACTTGGCAGGATCAGTAGG+AGG - chr4.2:43225656-43225675 None:intergenic 50.0%
AGCTGATGCAGTGACAAGCA+TGG + chr4.2:43227063-43227082 MS.gene004158:CDS 50.0%
GACCCAGTTTATGGTTGTGC+AGG + chr4.2:43227112-43227131 MS.gene004158:CDS 50.0%
GCATCAGCTCTTTGAGCTTC+TGG - chr4.2:43227052-43227071 None:intergenic 50.0%
GCTCCTGCACAACCATAAAC+TGG - chr4.2:43227118-43227137 None:intergenic 50.0%
! GACAACACTAGCCTATGCTC+AGG + chr4.2:43227361-43227380 MS.gene004158:CDS 50.0%
!! GAGCAACAAGATTGGCTTGC+TGG - chr4.2:43227222-43227241 None:intergenic 50.0%
AAGGAGAGAGGCCCACCATT+AGG - chr4.2:43226647-43226666 None:intergenic 55.0%
GCTCAGGGTGGGATACACTT+TGG + chr4.2:43227377-43227396 MS.gene004158:CDS 55.0%
GTGTATCCCACCCTGAGCAT+AGG - chr4.2:43227375-43227394 None:intergenic 55.0%
! ACACTAGCCTATGCTCAGGG+TGG + chr4.2:43227365-43227384 MS.gene004158:CDS 55.0%
! CACTAGCCTATGCTCAGGGT+GGG + chr4.2:43227366-43227385 MS.gene004158:CDS 55.0%
GTACGGGGGCTCCATATCCA+TGG - chr4.2:43226562-43226581 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 43225533 43227405 43225533 ID=MS.gene004158
chr4.2 mRNA 43225533 43227405 43225533 ID=MS.gene004158.t1;Parent=MS.gene004158
chr4.2 exon 43225533 43225727 43225533 ID=MS.gene004158.t1.exon1;Parent=MS.gene004158.t1
chr4.2 CDS 43225533 43225727 43225533 ID=cds.MS.gene004158.t1;Parent=MS.gene004158.t1
chr4.2 exon 43227043 43227405 43227043 ID=MS.gene004158.t1.exon2;Parent=MS.gene004158.t1
chr4.2 CDS 43227043 43227405 43227043 ID=cds.MS.gene004158.t1;Parent=MS.gene004158.t1
Gene Sequence

>MS.gene004158

ATGGAGAGTGCTACTAGTGACACTTTCTCTGCTCAAGCTGTTGTTATGAGTCCTTGTGCTGCTTGCAAGATTTTGAGGAGAAGATGTGCTGCTGATAAATGTGTTTTGGCACCTTATTTTCCTCCTACTGATCCTGCCAAGTTTACCATTGCTCATAGAGTTTTTGGTGCTAGCAACATCATCAAATTCTTACAGGTACTTAATTATACTAGAAAATGTTGAAATATTGGCGTATATTTAGAGACGAGAAAATGAGAAAACTATCTTTAATTTTGTCTGTGTGTTATTAGAGACACAATTAAATGAAAAGTTTTTATTTTCTGTCTCTAATTTTCGTCGCTATTTCAAGTTTTTATGATTCAATTATTTGTTTATAAATTTTCAAAATCTTTGTTCAATTACTTATTTCATTGTCTTAATTTTGCTTCAATATTGAGCTCACAATTAAAAGAACTTCTACGTTTGTTTATAAATGTTTGTGCTATTTAAGTGAACATTAGAGAAACAAAACTAGTGAATCTTGAAGATGTGTTCATATATTTTACACATATATTTATATAAGCATTAATTTGTGCAATTTTAATATTGATTTGTAGGATTATATAGAGTTCAACGTTTTTCTTTTATTTGAATCAATGATGAAAGATGGAATAATAATATATAGGAATCTCACGATTATTAAGTTTTAAGTTTGCAATTAGCAAAAATGCATAGTTTTTAATAAATTGTTGTGATCTAAGTAAGTGGTAAGAGGATAGAGTCTTTTAATATGTAATTAGAAGTTTGATTTCTAACTCATACGTATCAAGATTTACTTTTTGAGGAACAAGCTCTATATACATTCAACATTATCAAAGGAATTTTCTTGAGTTAGATTTACAAGAATCTAGAAAAACATAAAACAATAACACAAAATAGAAACAAAACAAAAAAATTTAGGAGTGAAACATGATAAGGAAGCATACATGGTTGAGATTTTGTTGAGGTTCCAAATCATGTAAAGGTCCTGCCCTTTTTTATTATGGTCCATGGATATGGAGCCCCCGTACTTTCCGTTATAATGCCTCAAATAAATGCATTGTCAACCACCTAATAATTCTAACAAATAGTACCTAATGGTGGGCCTCTCTCCTTGTAGTCATACATAACACTTCAAAATTCCAAATCTTGACCTAAAAATAGAAGCTAAAGAATAGGAGGAATAAAAAAAAAAAAACAATTTCTTTAATGTGAATGTCATCAATTAAAACAAAACCAAGTGTAAACGAAGTGGCTAATGAACTTTGATTAAAAATGAATCGATCAGAAGTATTTAAGTTAAAATCTTGATAGCGACTGTTGATTAGGTTTTACTTATCTTTTAACTGAACTCTGAAATACATGACTAATTTTTCTTGAAAAACCTAAAGGTTTAAGGAAAAAAAATGAAAACATAGAATAATCGTCTATCTCAAAGATATAGAAACTTATGCATTTTATCATCTAAACTTTTTGTTGTTGAAAAAAATAGGAACTTCCAGAAGCTCAAAGAGCTGATGCAGTGACAAGCATGGTTTATGAGGCTAGTGCAAGAGTTAGAGACCCAGTTTATGGTTGTGCAGGAGCAATTTGTCAACTCCAAAAGCAAGTCAATGAGCTTCAAGCACAACTAGCCAAATCACAAGCTGAGGTTGTAAACATGAAACTCCAGCAAGCCAATCTTGTTGCTCTATTTTGCATGGAAATGTCACAAAGTCCACAGGAATCACCACAACAGTTATCAGTGGATGATTTCATTCAAAGTCCTTCCCATAGCAATGGACATCATATCGGTTTGAACTTTCTTGATGACAACACTAGCCTATGCTCAGGGTGGGATACACTTTGGACATGA
Protein sequence

>MS.gene004158.t1

MESATSDTFSAQAVVMSPCAACKILRRRCAADKCVLAPYFPPTDPAKFTIAHRVFGASNIIKFLQELPEAQRADAVTSMVYEASARVRDPVYGCAGAICQLQKQVNELQAQLAKSQAEVVNMKLQQANLVALFCMEMSQSPQESPQQLSVDDFIQSPSHSNGHHIGLNFLDDNTSLCSGWDTLWT