Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004178.t1 | XP_024631664.1 | 100 | 80 | 0 | 0 | 1 | 80 | 579 | 658 | 1.20E-37 | 165.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004178.t1 | F4HY56 | 75.0 | 80 | 20 | 0 | 1 | 80 | 558 | 637 | 9.4e-31 | 133.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004178.t1 | G7ISG2 | 100.0 | 80 | 0 | 0 | 1 | 80 | 579 | 658 | 8.8e-38 | 165.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene004178.t1 | TR | DDT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004178.t1 | MTR_2g014490 | 100.000 | 80 | 0 | 0 | 1 | 80 | 579 | 658 | 7.26e-53 | 176 |
| MS.gene004178.t1 | MTR_2g014490 | 100.000 | 80 | 0 | 0 | 1 | 80 | 591 | 670 | 8.01e-53 | 176 |
| MS.gene004178.t1 | MTR_4g088375 | 91.250 | 80 | 7 | 0 | 1 | 80 | 272 | 351 | 2.10e-48 | 163 |
| MS.gene004178.t1 | MTR_4g088375 | 91.250 | 80 | 7 | 0 | 1 | 80 | 491 | 570 | 2.15e-48 | 163 |
| MS.gene004178.t1 | MTR_4g088375 | 91.250 | 80 | 7 | 0 | 1 | 80 | 525 | 604 | 2.15e-48 | 163 |
| MS.gene004178.t1 | MTR_4g088375 | 91.250 | 80 | 7 | 0 | 1 | 80 | 491 | 570 | 2.27e-48 | 163 |
| MS.gene004178.t1 | MTR_4g078860 | 75.000 | 80 | 20 | 0 | 1 | 80 | 556 | 635 | 8.61e-41 | 141 |
| MS.gene004178.t1 | MTR_4g078860 | 75.000 | 80 | 20 | 0 | 1 | 80 | 556 | 635 | 8.62e-41 | 141 |
| MS.gene004178.t1 | MTR_4g078860 | 75.000 | 80 | 20 | 0 | 1 | 80 | 530 | 609 | 8.63e-41 | 141 |
| MS.gene004178.t1 | MTR_4g078860 | 75.000 | 80 | 20 | 0 | 1 | 80 | 556 | 635 | 8.64e-41 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004178.t1 | AT1G28420 | 75.000 | 80 | 20 | 0 | 1 | 80 | 558 | 637 | 4.58e-41 | 142 |
| MS.gene004178.t1 | AT5G44180 | 77.500 | 80 | 18 | 0 | 1 | 80 | 523 | 602 | 3.23e-40 | 140 |
| MS.gene004178.t1 | AT5G44180 | 77.500 | 80 | 18 | 0 | 1 | 80 | 523 | 602 | 3.23e-40 | 140 |
| MS.gene004178.t1 | AT5G44180 | 77.500 | 80 | 18 | 0 | 1 | 80 | 523 | 602 | 4.89e-40 | 139 |
Find 0 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAAACTTAATAATAAC+AGG | - | 15696:2104-2123 | MS.gene004178:intron | 10.0% |
| !!! | ACTATTTTTCTTGCAATTTT+TGG | - | 15696:2063-2082 | MS.gene004178:CDS | 20.0% |
| ! | ACAAATTCATCAAGAGTAAA+AGG | + | 15696:1980-1999 | MS.gene004178:intergenic | 25.0% |
| ACTTGCAGTACAAAATCATT+AGG | + | 15696:2041-2060 | MS.gene004178:intergenic | 30.0% | |
| ATAACAGGATTCAAGATTGT+TGG | - | 15696:2119-2138 | MS.gene004178:intron | 30.0% | |
| CAGTACAAAATCATTAGGAA+AGG | + | 15696:2036-2055 | MS.gene004178:intergenic | 30.0% | |
| CATGATTATGTGAGTAAATC+TGG | - | 15696:2010-2029 | MS.gene004178:CDS | 30.0% | |
| TAACAGGATTCAAGATTGTT+GGG | - | 15696:2120-2139 | MS.gene004178:intron | 30.0% | |
| !!! | GCAATTTTTGGCTCATTTAT+GGG | - | 15696:2075-2094 | MS.gene004178:intron | 30.0% |
| !!! | TGCAATTTTTGGCTCATTTA+TGG | - | 15696:2074-2093 | MS.gene004178:intron | 30.0% |
| AAGACATTGAAGATGTTGCA+AGG | - | 15696:2178-2197 | MS.gene004178:intron | 35.0% | |
| TTCACTCCTAATCCTGTAGA+AGG | + | 15696:2207-2226 | MS.gene004178:intergenic | 40.0% | |
| TTGCTGATGCTCTTGAGTTA+TGG | - | 15696:1954-1973 | MS.gene004178:CDS | 40.0% | |
| !! | TAACTCAAGAGCATCAGCAA+AGG | + | 15696:1954-1973 | MS.gene004178:intergenic | 40.0% |
| AGGACACCTTCTACAGGATT+AGG | - | 15696:2198-2217 | MS.gene004178:CDS | 45.0% | |
| GGATTAGGAGTGAACCAGAA+TGG | - | 15696:2213-2232 | MS.gene004178:CDS | 45.0% | |
| AATGGAGCTGCTAATCCTGC+CGG | - | 15696:2231-2250 | MS.gene004178:CDS | 50.0% | |
| GTTGCAAGGACACCTTCTAC+AGG | - | 15696:2192-2211 | MS.gene004178:CDS | 50.0% | |
| ! | CAGGATTAGCAGCTCCATTC+TGG | + | 15696:2230-2249 | MS.gene004178:intergenic | 50.0% |
| CACAATCTCTGGATGGCCAC+CGG | + | 15696:2253-2272 | MS.gene004178:intergenic | 55.0% | |
| GGCCATCCAGAGATTGTGGA+AGG | - | 15696:2255-2274 | MS.gene004178:CDS | 55.0% | |
| CGGTGGCCATCCAGAGATTG+TGG | - | 15696:2251-2270 | MS.gene004178:CDS | 60.0% | |
| ATCTCTGGATGGCCACCGGC+AGG | + | 15696:2249-2268 | MS.gene004178:intergenic | 65.0% | |
| GGAGCTGCTAATCCTGCCGG+TGG | - | 15696:2234-2253 | MS.gene004178:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15696 | gene | 1932 | 2278 | 1932 | ID=MS.gene004178 |
| 15696 | mRNA | 1932 | 2278 | 1932 | ID=MS.gene004178.t1;Parent=MS.gene004178 |
| 15696 | exon | 2192 | 2278 | 2192 | ID=MS.gene004178.t1.exon1;Parent=MS.gene004178.t1 |
| 15696 | CDS | 2192 | 2278 | 2192 | ID=cds.MS.gene004178.t1;Parent=MS.gene004178.t1 |
| 15696 | exon | 1932 | 2084 | 1932 | ID=MS.gene004178.t1.exon2;Parent=MS.gene004178.t1 |
| 15696 | CDS | 1932 | 2084 | 1932 | ID=cds.MS.gene004178.t1;Parent=MS.gene004178.t1 |
| Gene Sequence |
| Protein sequence |
