AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004189

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004189.t1 MTR_4g088125 74.043 470 107 3 3 461 5 470 0.0 714
MS.gene004189.t1 MTR_4g079240 45.984 498 200 10 3 437 6 497 8.72e-138 405
MS.gene004189.t1 MTR_2g014870 93.701 127 5 2 1 124 1 127 8.83e-76 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004189.t1 AT1G28520 65.021 466 138 6 3 450 6 464 0.0 591
MS.gene004189.t1 AT1G28520 65.021 466 138 6 3 450 6 464 0.0 591
MS.gene004189.t1 AT1G28520 65.021 466 138 6 3 450 6 464 0.0 591
MS.gene004189.t1 AT1G28520 65.021 466 138 6 3 450 6 464 0.0 591
MS.gene004189.t1 AT1G28520 62.232 466 135 7 3 450 6 448 0.0 553
MS.gene004189.t1 AT2G42400 49.091 440 177 10 10 416 9 434 3.57e-124 369

Find 115 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTCATCACTTGCTTTC+TGG -0.000768 2.4:+69983901 None:intergenic
GTTGCCGCTCCCTCGCATTC+TGG 0.120554 2.4:+69984180 None:intergenic
GTATGTACTCATCCATTAAT+AGG 0.244748 2.4:+69984622 None:intergenic
TTGGAACTAAAGCTCGTTAA+TGG 0.245316 2.4:-69983767 MS.gene004189:CDS
TCAGCATGATTCCCTATTAA+TGG 0.250839 2.4:-69984634 MS.gene004189:CDS
CATAAAGATGCCACTCAAAA+TGG 0.277276 2.4:+69983831 None:intergenic
CGAGTTGTTAGTTGATTTAC+TGG 0.280780 2.4:+69984581 None:intergenic
TTGCCGCTCCCTCGCATTCT+GGG 0.309111 2.4:+69984181 None:intergenic
AGGTCGTTGCCTGATTATAA+TGG 0.319828 2.4:-69983941 MS.gene004189:CDS
GGTAGATAGGTAGGTATATA+AGG 0.325810 2.4:+69984444 None:intergenic
GTCAGCCTTGGCATTAGGTT+TGG 0.334729 2.4:+69983628 None:intergenic
GTCTGCATATTCTGATCATT+AGG 0.347297 2.4:+69985159 None:intergenic
GAGTACATACACTCCATCTT+AGG 0.353453 2.4:-69984609 MS.gene004189:CDS
CCCATGTCAGCCTTGGCATT+AGG 0.366697 2.4:+69983623 None:intergenic
AGGGCAGATGCTTGGTAGAT+AGG 0.368518 2.4:+69984431 None:intergenic
AAGCAAGTGATGAAAGAGTT+TGG 0.371141 2.4:-69983896 MS.gene004189:CDS
TCCTGCTTCTTCTTTGCAGC+AGG 0.376005 2.4:-69985420 MS.gene004189:intron
ATCATTTGTTATCTTTGTCC+TGG 0.382306 2.4:+69983733 None:intergenic
AGCAGAATGTCTGTTATCAT+AGG 0.386907 2.4:+69983661 None:intergenic
AGGTTCAAAATGTGCACTTT+GGG 0.394325 2.4:-69984391 MS.gene004189:CDS
CCTCCTCCTTCCGCTTTCCT+AGG 0.410735 2.4:-69984411 MS.gene004189:CDS
TGCCACTCAAAATGGTTTAG+TGG 0.430399 2.4:+69983839 None:intergenic
TCAAAATGGTTTAGTGGTTG+TGG 0.432899 2.4:+69983845 None:intergenic
CCTTCCACTCACGAAGCATC+TGG 0.436154 2.4:+69985462 None:intergenic
TCATGCTTAGGCACAGCTAA+TGG 0.436229 2.4:+69985183 None:intergenic
TAGGTTCAAAATGTGCACTT+TGG 0.438695 2.4:-69984392 MS.gene004189:CDS
GTGATGCATGTGCGTTATAT+CGG 0.439847 2.4:-69983790 MS.gene004189:CDS
CTGCAGTAGCCTACATATAT+CGG 0.447566 2.4:+69985236 None:intergenic
GGTCGTTGCCTGATTATAAT+GGG 0.452127 2.4:-69983940 MS.gene004189:CDS
TATGTACTCATCCATTAATA+GGG 0.456560 2.4:+69984623 None:intergenic
TGGAACTAAAGCTCGTTAAT+GGG 0.459138 2.4:-69983766 MS.gene004189:CDS
GCTGCTCTTGCTTTAAATGA+AGG 0.460368 2.4:-69984309 MS.gene004189:CDS
CATCCCCGCCCATTATAATC+AGG 0.465616 2.4:+69983932 None:intergenic
TGATCATTAGGTTCATGCTT+AGG 0.466635 2.4:+69985171 None:intergenic
TAGCCCCAGTTCTACGACCT+GGG 0.466781 2.4:-69984275 MS.gene004189:CDS
TGCTTTCTTACAGCTACCCT+TGG 0.466878 2.4:+69985613 None:intergenic
ACCTAATGCCAAGGCTGACA+TGG 0.476238 2.4:-69983624 MS.gene004189:CDS
CGTAGAACTGGGGCTATGCC+TGG 0.483033 2.4:+69984282 None:intergenic
TGATCTGCAGTTTGCTAGGA+AGG 0.490071 2.4:-69985528 MS.gene004189:CDS
CTGATTATAATGGGCGGGGA+TGG 0.493655 2.4:-69983931 MS.gene004189:CDS
TCCTAGCAAACTGCAGATCA+AGG 0.504979 2.4:+69985531 None:intergenic
GTTGCCTGATTATAATGGGC+GGG 0.508969 2.4:-69983936 MS.gene004189:CDS
TGGTTGTGGATCCATGTAGT+AGG 0.510429 2.4:+69983859 None:intergenic
CATCCCAGAATGCGAGGGAG+CGG 0.511232 2.4:-69984184 MS.gene004189:CDS
TATACTGCTTTCAGAGGCAC+AGG 0.514147 2.4:-69984489 MS.gene004189:CDS
TTTACTGGGAGCTCCTAAGA+TGG 0.517698 2.4:+69984596 None:intergenic
AACACCACCCATGTCAGCCT+TGG 0.521369 2.4:+69983616 None:intergenic
CCTAGGAAAGCGGAAGGAGG+AGG 0.522377 2.4:+69984411 None:intergenic
GAAGGAGGAGGGCAGATGCT+TGG 0.522630 2.4:+69984423 None:intergenic
CTCTCAACAAGATAGTCATA+TGG 0.523925 2.4:+69983566 None:intergenic
TGTGGATGTCGCGCTTCTTG+AGG 0.525115 2.4:-69985495 MS.gene004189:CDS
AGCATCACACAGGCTTATCA+AGG 0.532332 2.4:-69985594 MS.gene004189:CDS
CACAAGTTCAACCCTTCCGC+GGG 0.534065 2.4:+69984357 None:intergenic
CCAGATGCTTCGTGAGTGGA+AGG 0.543218 2.4:-69985462 MS.gene004189:CDS
TGATAACAGACATTCTGCTA+AGG 0.543724 2.4:-69983657 MS.gene004189:CDS
GGCACAGGCTTTAGTGAGGA+TGG 0.545649 2.4:-69984474 MS.gene004189:CDS
ACCTGCTGCAAAGAAGAAGC+AGG 0.547011 2.4:+69985419 None:intergenic
CATTCTCGACCGATATATGT+AGG 0.550090 2.4:-69985245 MS.gene004189:CDS
CAGATGCTTGGTAGATAGGT+AGG 0.551289 2.4:+69984435 None:intergenic
GCTTTAAATGAAGGACAACC+AGG 0.554170 2.4:-69984300 MS.gene004189:CDS
GCAGAATGTCTGTTATCATA+GGG 0.554543 2.4:+69983662 None:intergenic
AGTTAAGGATAATTTACTGT+TGG 0.554729 2.4:-69984244 MS.gene004189:CDS
ATAGCCCCAGTTCTACGACC+TGG 0.557433 2.4:-69984276 MS.gene004189:CDS
TGAGGTTTAGATGATTTCCA+AGG 0.559690 2.4:-69985630 None:intergenic
ATAGGGAATCATGCTGATGT+TGG 0.560720 2.4:+69984640 None:intergenic
TTAATGGGAAGAAGAGCTCC+AGG 0.561240 2.4:-69983751 MS.gene004189:CDS
TCCTTGATCTGCAGTTTGCT+AGG 0.561339 2.4:-69985532 MS.gene004189:CDS
CAGTAAATCAACTAACAACT+CGG 0.567663 2.4:-69984580 MS.gene004189:CDS
ACACAAGTTCAACCCTTCCG+CGG 0.569071 2.4:+69984356 None:intergenic
TGCAAAGAAGAAGCAGGAGA+AGG 0.571409 2.4:+69985425 None:intergenic
AAGTCTCCGTGGAATGCACC+TGG 0.578340 2.4:-69984153 MS.gene004189:intron
CTAGGAAAGCGGAAGGAGGA+GGG 0.578498 2.4:+69984412 None:intergenic
TTGCCTGATTATAATGGGCG+GGG 0.584990 2.4:-69983935 MS.gene004189:CDS
AAGTGATGAAAGAGTTTGGA+GGG 0.587404 2.4:-69983892 MS.gene004189:CDS
GACTGTTCAAGACCCGCGGA+AGG 0.587498 2.4:-69984369 MS.gene004189:CDS
GCTCTTAGTGCAAAGACAGA+AGG 0.588265 2.4:-69984219 MS.gene004189:CDS
GATAACAGACATTCTGCTAA+GGG 0.588460 2.4:-69983656 MS.gene004189:CDS
ACAAGATAGTCATATGGTGA+TGG 0.593941 2.4:+69983572 None:intergenic
CAGTTACTGATCTTCAGAAG+CGG 0.596139 2.4:-69983709 MS.gene004189:CDS
TTCACCAGATGCTTCGTGAG+TGG 0.596799 2.4:-69985466 MS.gene004189:CDS
TGCATGTGCGTTATATCGGT+TGG 0.607322 2.4:-69983786 MS.gene004189:CDS
AACTGTTATACTGCTTTCAG+AGG 0.607971 2.4:-69984495 MS.gene004189:CDS
CAGAGCCAAACCTAATGCCA+AGG 0.608334 2.4:-69983633 MS.gene004189:CDS
AGGAGAAGGCTCATTGAGCT+CGG 0.609324 2.4:+69985439 None:intergenic
AATCAACTAACAACTCGGAT+AGG 0.609346 2.4:-69984575 MS.gene004189:CDS
AGCCCCAGTTCTACGACCTG+GGG 0.611570 2.4:-69984274 MS.gene004189:CDS
CCTAATGCCAAGGCTGACAT+GGG 0.613097 2.4:-69983623 MS.gene004189:CDS
CTAATACCAGGTGCATTCCA+CGG 0.614138 2.4:+69984147 None:intergenic
TGTCGGCATCCCAGAATGCG+AGG 0.614404 2.4:-69984190 MS.gene004189:CDS
GCTACTGCAGCTCTGTGAAG+AGG 0.620067 2.4:-69985223 MS.gene004189:CDS
AAGACAGAAGGAAAAGATGT+CGG 0.620071 2.4:-69984207 MS.gene004189:CDS
GAGGTTTAGATGATTTCCAA+GGG 0.620877 2.4:-69985629 None:intergenic
CAGAGGCACAGGCTTTAGTG+AGG 0.620913 2.4:-69984478 MS.gene004189:CDS
AGGCACAGCTAATGGACTAG+TGG 0.628612 2.4:+69985191 None:intergenic
CGTTGCCTGATTATAATGGG+CGG 0.632470 2.4:-69983937 MS.gene004189:CDS
TTTGAACCTAGGAAAGCGGA+AGG 0.634334 2.4:+69984405 None:intergenic
GCTGAAGAGATCCTACTACA+TGG 0.634906 2.4:-69983870 MS.gene004189:CDS
CAACTAACAACTCGGATAGG+CGG 0.636833 2.4:-69984572 MS.gene004189:CDS
TCTCGAAGAGCATTTGAGAG+TGG 0.636901 2.4:-69983977 MS.gene004189:CDS
ACTGTTCAAGACCCGCGGAA+GGG 0.637959 2.4:-69984368 MS.gene004189:CDS
TTGGGACTGTTCAAGACCCG+CGG 0.641938 2.4:-69984373 MS.gene004189:CDS
GAAGCGCGACATCCACACTA+CGG 0.643778 2.4:+69985501 None:intergenic
TCACATGCAGATCATCTACA+TGG 0.644881 2.4:+69985558 None:intergenic
GAACCTAGGAAAGCGGAAGG+AGG 0.646171 2.4:+69984408 None:intergenic
TCTGTTATCATAGGGAAACT+CGG 0.647001 2.4:+69983670 None:intergenic
CGGCAACTGCAAAGTCTCCG+TGG 0.652685 2.4:-69984164 MS.gene004189:CDS
GTCGGCATCCCAGAATGCGA+GGG 0.657248 2.4:-69984189 MS.gene004189:CDS
TAGGAAGGAGAGCCGTAGTG+TGG 0.661628 2.4:-69985513 MS.gene004189:CDS
GAGTTGTTAGTTGATTTACT+GGG 0.663415 2.4:+69984582 None:intergenic
CAAGTGATGAAAGAGTTTGG+AGG 0.680936 2.4:-69983893 MS.gene004189:CDS
GCTGTAAGAAAGCATCACAC+AGG 0.691286 2.4:-69985604 MS.gene004189:CDS
ACACAGGCTTATCAAGGACA+AGG 0.694542 2.4:-69985588 MS.gene004189:CDS
AGAGTGGAAACAGAAAGCAA+AGG 0.717117 2.4:-69983961 MS.gene004189:CDS
GCCCCAGTTCTACGACCTGG+GGG 0.725582 2.4:-69984273 MS.gene004189:CDS
AATGCCAAGGCTGACATGGG+TGG 0.739584 2.4:-69983620 MS.gene004189:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTATTTTTTAGAGAAA+AGG + chr2.4:69984302-69984321 None:intergenic 10.0%
!! AAACACATAAATAGTTTAAT+AGG + chr2.4:69985066-69985085 None:intergenic 15.0%
!!! TTTTATTTTTTCTGTAGTAT+TGG - chr2.4:69984123-69984142 MS.gene004189:intron 15.0%
!! AAATCTGCATATTAAAGTTA+AGG + chr2.4:69983791-69983810 None:intergenic 20.0%
!! TAATTCATATACTAGAGTAA+GGG - chr2.4:69984194-69984213 MS.gene004189:CDS 20.0%
!! TTAATATGCAGATTTAATTG+TGG - chr2.4:69983796-69983815 MS.gene004189:CDS 20.0%
!! TTAATTCATATACTAGAGTA+AGG - chr2.4:69984193-69984212 MS.gene004189:CDS 20.0%
!!! GTTGTATTGTTTTTTATATG+TGG - chr2.4:69984273-69984292 MS.gene004189:CDS 20.0%
!!! TTATTTTCAATTGACAGAAA+TGG - chr2.4:69984329-69984348 MS.gene004189:CDS 20.0%
! AGTTAAGGATAATTTACTGT+TGG - chr2.4:69984923-69984942 MS.gene004189:intron 25.0%
! ATGATGCATAAGAACAAATT+GGG + chr2.4:69984421-69984440 None:intergenic 25.0%
! ATTTAATTGTGGATTTGTGA+TGG - chr2.4:69983807-69983826 MS.gene004189:CDS 25.0%
! GAGAAAATTGGTATTAACTA+AGG + chr2.4:69984383-69984402 None:intergenic 25.0%
! TATGATGCATAAGAACAAAT+TGG + chr2.4:69984422-69984441 None:intergenic 25.0%
! TATGATTGTAACAAGTTTCT+TGG - chr2.4:69984486-69984505 MS.gene004189:CDS 25.0%
! TATGTACTCATCCATTAATA+GGG + chr2.4:69984547-69984566 None:intergenic 25.0%
!! TTTGATCTTTTTGTTCTTGA+GGG - chr2.4:69985133-69985152 MS.gene004189:intron 25.0%
!!! CGACATTTTGATTCAAAATT+AGG + chr2.4:69984621-69984640 None:intergenic 25.0%
AACTTGAATGGCAATATTTG+TGG - chr2.4:69983839-69983858 MS.gene004189:CDS 30.0%
CAGTAAATCAACTAACAACT+CGG - chr2.4:69984587-69984606 MS.gene004189:CDS 30.0%
CTTTGTGATTTGATCATAAC+TGG - chr2.4:69984094-69984113 MS.gene004189:intron 30.0%
GTATGTACTCATCCATTAAT+AGG + chr2.4:69984548-69984567 None:intergenic 30.0%
! ATTTGAGTTTGCCATGTTTA+TGG - chr2.4:69983873-69983892 MS.gene004189:CDS 30.0%
! CTTTGATCTTTTTGTTCTTG+AGG - chr2.4:69985132-69985151 MS.gene004189:intron 30.0%
! GAGTTGTTAGTTGATTTACT+GGG + chr2.4:69984588-69984607 None:intergenic 30.0%
! TTGATCTTTTTGTTCTTGAG+GGG - chr2.4:69985134-69985153 MS.gene004189:CDS 30.0%
! TTTGAGTTTGCCATGTTTAT+GGG - chr2.4:69983874-69983893 MS.gene004189:CDS 30.0%
!! ATCATTTGTTATCTTTGTCC+TGG + chr2.4:69985437-69985456 None:intergenic 30.0%
AACTGTTATACTGCTTTCAG+AGG - chr2.4:69984672-69984691 MS.gene004189:intron 35.0%
AAGACAGAAGGAAAAGATGT+CGG - chr2.4:69984960-69984979 MS.gene004189:intron 35.0%
AAGCAAGTGATGAAAGAGTT+TGG - chr2.4:69985271-69985290 MS.gene004189:intron 35.0%
AAGTGATGAAAGAGTTTGGA+GGG - chr2.4:69985275-69985294 MS.gene004189:intron 35.0%
AATCAACTAACAACTCGGAT+AGG - chr2.4:69984592-69984611 MS.gene004189:CDS 35.0%
ACAAGATAGTCATATGGTGA+TGG + chr2.4:69985598-69985617 None:intergenic 35.0%
AGCAGAATGTCTGTTATCAT+AGG + chr2.4:69985509-69985528 None:intergenic 35.0%
AGGTTCAAAATGTGCACTTT+GGG - chr2.4:69984776-69984795 MS.gene004189:intron 35.0%
CATAAAGATGCCACTCAAAA+TGG + chr2.4:69985339-69985358 None:intergenic 35.0%
CATGTTTATGGGTGATGATA+GGG - chr2.4:69983885-69983904 MS.gene004189:CDS 35.0%
CTCTCAACAAGATAGTCATA+TGG + chr2.4:69985604-69985623 None:intergenic 35.0%
GATAACAGACATTCTGCTAA+GGG - chr2.4:69985511-69985530 MS.gene004189:CDS 35.0%
GCAGAATGTCTGTTATCATA+GGG + chr2.4:69985508-69985527 None:intergenic 35.0%
GCATTCTACATTCTAATACC+AGG + chr2.4:69985035-69985054 None:intergenic 35.0%
GGTAGATAGGTAGGTATATA+AGG + chr2.4:69984726-69984745 None:intergenic 35.0%
GTCTGCATATTCTGATCATT+AGG + chr2.4:69984011-69984030 None:intergenic 35.0%
TAGGTTCAAAATGTGCACTT+TGG - chr2.4:69984775-69984794 MS.gene004189:intron 35.0%
TCAAAATGGTTTAGTGGTTG+TGG + chr2.4:69985325-69985344 None:intergenic 35.0%
TCAGCATGATTCCCTATTAA+TGG - chr2.4:69984533-69984552 MS.gene004189:CDS 35.0%
TCTGTTATCATAGGGAAACT+CGG + chr2.4:69985500-69985519 None:intergenic 35.0%
TGATAACAGACATTCTGCTA+AGG - chr2.4:69985510-69985529 MS.gene004189:CDS 35.0%
TGATCATTAGGTTCATGCTT+AGG + chr2.4:69983999-69984018 None:intergenic 35.0%
TGGAACTAAAGCTCGTTAAT+GGG - chr2.4:69985401-69985420 MS.gene004189:intron 35.0%
TGGACACATGTAAACTTGAA+TGG - chr2.4:69983827-69983846 MS.gene004189:CDS 35.0%
TGTATGAATAATCGCTGAAG+AGG + chr2.4:69984073-69984092 None:intergenic 35.0%
TTGGAACTAAAGCTCGTTAA+TGG - chr2.4:69985400-69985419 MS.gene004189:intron 35.0%
! AAAGTGCACATTTTGAACCT+AGG + chr2.4:69984776-69984795 None:intergenic 35.0%
! AACCACTAAACCATTTTGAG+TGG - chr2.4:69985326-69985345 MS.gene004189:intron 35.0%
! ACATTTTGAACCTAGGAAAG+CGG + chr2.4:69984769-69984788 None:intergenic 35.0%
! CGAGTTGTTAGTTGATTTAC+TGG + chr2.4:69984589-69984608 None:intergenic 35.0%
! TGAGTTGTTGGTGAGAAAAT+TGG + chr2.4:69984395-69984414 None:intergenic 35.0%
! TGATCTTTTTGTTCTTGAGG+GGG - chr2.4:69985135-69985154 MS.gene004189:CDS 35.0%
AATGGAGAGCAAGGATTTGA+TGG - chr2.4:69984347-69984366 MS.gene004189:CDS 40.0%
AGAGTGGAAACAGAAAGCAA+AGG - chr2.4:69985206-69985225 MS.gene004189:CDS 40.0%
AGGTCGTTGCCTGATTATAA+TGG - chr2.4:69985226-69985245 MS.gene004189:CDS 40.0%
ATAATCGCTGAAGAGGAGAA+AGG + chr2.4:69984066-69984085 None:intergenic 40.0%
ATAGGGAATCATGCTGATGT+TGG + chr2.4:69984530-69984549 None:intergenic 40.0%
ATTGACAGAAATGGAGAGCA+AGG - chr2.4:69984338-69984357 MS.gene004189:CDS 40.0%
CAAGTGATGAAAGAGTTTGG+AGG - chr2.4:69985274-69985293 MS.gene004189:intron 40.0%
CAGTTACTGATCTTCAGAAG+CGG - chr2.4:69985458-69985477 MS.gene004189:CDS 40.0%
CATTCTCGACCGATATATGT+AGG - chr2.4:69983922-69983941 MS.gene004189:CDS 40.0%
CCATGTTTATGGGTGATGAT+AGG - chr2.4:69983884-69983903 MS.gene004189:CDS 40.0%
CCTATCATCACCCATAAACA+TGG + chr2.4:69983887-69983906 None:intergenic 40.0%
CTCTTTCATCACTTGCTTTC+TGG + chr2.4:69985269-69985288 None:intergenic 40.0%
CTGCAGTAGCCTACATATAT+CGG + chr2.4:69983934-69983953 None:intergenic 40.0%
GAGTACATACACTCCATCTT+AGG - chr2.4:69984558-69984577 MS.gene004189:CDS 40.0%
GGTCGTTGCCTGATTATAAT+GGG - chr2.4:69985227-69985246 MS.gene004189:CDS 40.0%
GTGATGCATGTGCGTTATAT+CGG - chr2.4:69985377-69985396 MS.gene004189:intron 40.0%
TACTAGAGTAAGGGATGCTT+TGG - chr2.4:69984203-69984222 MS.gene004189:CDS 40.0%
TCACATGCAGATCATCTACA+TGG + chr2.4:69983612-69983631 None:intergenic 40.0%
TGCCACTCAAAATGGTTTAG+TGG + chr2.4:69985331-69985350 None:intergenic 40.0%
! GCTGCTCTTGCTTTAAATGA+AGG - chr2.4:69984858-69984877 MS.gene004189:intron 40.0%
! GCTTTAAATGAAGGACAACC+AGG - chr2.4:69984867-69984886 MS.gene004189:intron 40.0%
!! CAAATTGGGCTTTGAGTTGT+TGG + chr2.4:69984407-69984426 None:intergenic 40.0%
ACACAGGCTTATCAAGGACA+AGG - chr2.4:69983579-69983598 MS.gene004189:CDS 45.0%
ACCTGGGGGAATTGAAGTTA+AGG - chr2.4:69984908-69984927 MS.gene004189:intron 45.0%
AGCATCACACAGGCTTATCA+AGG - chr2.4:69983573-69983592 MS.gene004189:CDS 45.0%
CAACTAACAACTCGGATAGG+CGG - chr2.4:69984595-69984614 MS.gene004189:CDS 45.0%
CAGATGCTTGGTAGATAGGT+AGG + chr2.4:69984735-69984754 None:intergenic 45.0%
CTAATACCAGGTGCATTCCA+CGG + chr2.4:69985023-69985042 None:intergenic 45.0%
GCTCTTAGTGCAAAGACAGA+AGG - chr2.4:69984948-69984967 MS.gene004189:intron 45.0%
GCTGAAGAGATCCTACTACA+TGG - chr2.4:69985297-69985316 MS.gene004189:intron 45.0%
GTTTATGGGTGATGATAGGG+TGG - chr2.4:69983888-69983907 MS.gene004189:CDS 45.0%
TCATGCTTAGGCACAGCTAA+TGG + chr2.4:69983987-69984006 None:intergenic 45.0%
TCCTAGCAAACTGCAGATCA+AGG + chr2.4:69983639-69983658 None:intergenic 45.0%
TCCTTAACTTCAATTCCCCC+AGG + chr2.4:69984912-69984931 None:intergenic 45.0%
TCTTGAGGGGGAGACTATTA+GGG - chr2.4:69985147-69985166 MS.gene004189:CDS 45.0%
TGCAAAGAAGAAGCAGGAGA+AGG + chr2.4:69983745-69983764 None:intergenic 45.0%
TGCATGTGCGTTATATCGGT+TGG - chr2.4:69985381-69985400 MS.gene004189:intron 45.0%
TGCTTTCTTACAGCTACCCT+TGG + chr2.4:69983557-69983576 None:intergenic 45.0%
TGGTTGTGGATCCATGTAGT+AGG + chr2.4:69985311-69985330 None:intergenic 45.0%
TTAATGGGAAGAAGAGCTCC+AGG - chr2.4:69985416-69985435 MS.gene004189:intron 45.0%
TTCTTGAGGGGGAGACTATT+AGG - chr2.4:69985146-69985165 MS.gene004189:CDS 45.0%
TTTACTGGGAGCTCCTAAGA+TGG + chr2.4:69984574-69984593 None:intergenic 45.0%
TTTGAACCTAGGAAAGCGGA+AGG + chr2.4:69984765-69984784 None:intergenic 45.0%
! CGTTGCCTGATTATAATGGG+CGG - chr2.4:69985230-69985249 MS.gene004189:CDS 45.0%
! TATACTGCTTTCAGAGGCAC+AGG - chr2.4:69984678-69984697 MS.gene004189:intron 45.0%
! TCCTTGATCTGCAGTTTGCT+AGG - chr2.4:69983635-69983654 MS.gene004189:CDS 45.0%
!! AATCATGCTGATGTTGGCTC+TGG + chr2.4:69984524-69984543 None:intergenic 45.0%
!! GCTGTAAGAAAGCATCACAC+AGG - chr2.4:69983563-69983582 MS.gene004189:CDS 45.0%
!! GTTGCCTGATTATAATGGGC+GGG - chr2.4:69985231-69985250 MS.gene004189:CDS 45.0%
!! TCTCGAAGAGCATTTGAGAG+TGG - chr2.4:69985190-69985209 MS.gene004189:CDS 45.0%
!! TGATCTGCAGTTTGCTAGGA+AGG - chr2.4:69983639-69983658 MS.gene004189:CDS 45.0%
!! TTGCCTGATTATAATGGGCG+GGG - chr2.4:69985232-69985251 MS.gene004189:CDS 45.0%
ACACAAGTTCAACCCTTCCG+CGG + chr2.4:69984814-69984833 None:intergenic 50.0%
ACCTAATGCCAAGGCTGACA+TGG - chr2.4:69985543-69985562 MS.gene004189:CDS 50.0%
ACCTGCTGCAAAGAAGAAGC+AGG + chr2.4:69983751-69983770 None:intergenic 50.0%
AGGAGAAGGCTCATTGAGCT+CGG + chr2.4:69983731-69983750 None:intergenic 50.0%
AGGGCAGATGCTTGGTAGAT+AGG + chr2.4:69984739-69984758 None:intergenic 50.0%
CAGAGCCAAACCTAATGCCA+AGG - chr2.4:69985534-69985553 MS.gene004189:CDS 50.0%
CATCCCCGCCCATTATAATC+AGG + chr2.4:69985238-69985257 None:intergenic 50.0%
CCTAATGCCAAGGCTGACAT+GGG - chr2.4:69985544-69985563 MS.gene004189:CDS 50.0%
GTCAGCCTTGGCATTAGGTT+TGG + chr2.4:69985542-69985561 None:intergenic 50.0%
TCCTGCTTCTTCTTTGCAGC+AGG - chr2.4:69983747-69983766 MS.gene004189:CDS 50.0%
TTCACCAGATGCTTCGTGAG+TGG - chr2.4:69983701-69983720 MS.gene004189:CDS 50.0%
!! AGGCACAGCTAATGGACTAG+TGG + chr2.4:69983979-69983998 None:intergenic 50.0%
!! CTGATTATAATGGGCGGGGA+TGG - chr2.4:69985236-69985255 MS.gene004189:CDS 50.0%
AACACCACCCATGTCAGCCT+TGG + chr2.4:69985554-69985573 None:intergenic 55.0%
AAGTCTCCGTGGAATGCACC+TGG - chr2.4:69985014-69985033 MS.gene004189:intron 55.0%
AATGCCAAGGCTGACATGGG+TGG - chr2.4:69985547-69985566 MS.gene004189:CDS 55.0%
ACTGTTCAAGACCCGCGGAA+GGG - chr2.4:69984799-69984818 MS.gene004189:intron 55.0%
AGGGGGAGACTATTAGGGAG+TGG - chr2.4:69985152-69985171 MS.gene004189:CDS 55.0%
ATAGCCCCAGTTCTACGACC+TGG - chr2.4:69984891-69984910 MS.gene004189:intron 55.0%
ATTCCCCCAGGTCGTAGAAC+TGG + chr2.4:69984900-69984919 None:intergenic 55.0%
CACAAGTTCAACCCTTCCGC+GGG + chr2.4:69984813-69984832 None:intergenic 55.0%
CCAGATGCTTCGTGAGTGGA+AGG - chr2.4:69983705-69983724 MS.gene004189:CDS 55.0%
CCCATGTCAGCCTTGGCATT+AGG + chr2.4:69985547-69985566 None:intergenic 55.0%
CTAGGAAAGCGGAAGGAGGA+GGG + chr2.4:69984758-69984777 None:intergenic 55.0%
GAACCTAGGAAAGCGGAAGG+AGG + chr2.4:69984762-69984781 None:intergenic 55.0%
GAAGCGCGACATCCACACTA+CGG + chr2.4:69983669-69983688 None:intergenic 55.0%
GCTACTGCAGCTCTGTGAAG+AGG - chr2.4:69983944-69983963 MS.gene004189:CDS 55.0%
TAGCCCCAGTTCTACGACCT+GGG - chr2.4:69984892-69984911 MS.gene004189:intron 55.0%
TAGGAAGGAGAGCCGTAGTG+TGG - chr2.4:69983654-69983673 MS.gene004189:CDS 55.0%
TGTGGATGTCGCGCTTCTTG+AGG - chr2.4:69983672-69983691 MS.gene004189:CDS 55.0%
TTCCCCCAGGTCGTAGAACT+GGG + chr2.4:69984899-69984918 None:intergenic 55.0%
TTGGGACTGTTCAAGACCCG+CGG - chr2.4:69984794-69984813 MS.gene004189:intron 55.0%
! CAGAGGCACAGGCTTTAGTG+AGG - chr2.4:69984689-69984708 MS.gene004189:intron 55.0%
! CCTTCCACTCACGAAGCATC+TGG + chr2.4:69983708-69983727 None:intergenic 55.0%
! GGCACAGGCTTTAGTGAGGA+TGG - chr2.4:69984693-69984712 MS.gene004189:intron 55.0%
AGCCCCAGTTCTACGACCTG+GGG - chr2.4:69984893-69984912 MS.gene004189:intron 60.0%
CATCCCAGAATGCGAGGGAG+CGG - chr2.4:69984983-69985002 MS.gene004189:intron 60.0%
CCTAGGAAAGCGGAAGGAGG+AGG + chr2.4:69984759-69984778 None:intergenic 60.0%
CCTCCTCCTTCCGCTTTCCT+AGG - chr2.4:69984756-69984775 MS.gene004189:intron 60.0%
CGGCAACTGCAAAGTCTCCG+TGG - chr2.4:69985003-69985022 MS.gene004189:intron 60.0%
CGTAGAACTGGGGCTATGCC+TGG + chr2.4:69984888-69984907 None:intergenic 60.0%
GAAGGAGGAGGGCAGATGCT+TGG + chr2.4:69984747-69984766 None:intergenic 60.0%
GACTGTTCAAGACCCGCGGA+AGG - chr2.4:69984798-69984817 MS.gene004189:intron 60.0%
GTCGGCATCCCAGAATGCGA+GGG - chr2.4:69984978-69984997 MS.gene004189:intron 60.0%
TCCCCCAGGTCGTAGAACTG+GGG + chr2.4:69984898-69984917 None:intergenic 60.0%
TGTCGGCATCCCAGAATGCG+AGG - chr2.4:69984977-69984996 MS.gene004189:intron 60.0%
TTGCCGCTCCCTCGCATTCT+GGG + chr2.4:69984989-69985008 None:intergenic 60.0%
GCCCCAGTTCTACGACCTGG+GGG - chr2.4:69984894-69984913 MS.gene004189:intron 65.0%
GTTGCCGCTCCCTCGCATTC+TGG + chr2.4:69984990-69985009 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 69983547 69985642 69983547 ID=MS.gene004189
chr2.4 mRNA 69983547 69985642 69983547 ID=MS.gene004189.t1;Parent=MS.gene004189
chr2.4 exon 69985421 69985642 69985421 ID=MS.gene004189.t1.exon1;Parent=MS.gene004189.t1
chr2.4 CDS 69985421 69985642 69985421 ID=cds.MS.gene004189.t1;Parent=MS.gene004189.t1
chr2.4 exon 69985134 69985283 69985134 ID=MS.gene004189.t1.exon2;Parent=MS.gene004189.t1
chr2.4 CDS 69985134 69985283 69985134 ID=cds.MS.gene004189.t1;Parent=MS.gene004189.t1
chr2.4 exon 69984154 69984664 69984154 ID=MS.gene004189.t1.exon3;Parent=MS.gene004189.t1
chr2.4 CDS 69984154 69984664 69984154 ID=cds.MS.gene004189.t1;Parent=MS.gene004189.t1
chr2.4 exon 69983547 69984061 69983547 ID=MS.gene004189.t1.exon4;Parent=MS.gene004189.t1
chr2.4 CDS 69983547 69984061 69983547 ID=cds.MS.gene004189.t1;Parent=MS.gene004189.t1
Gene Sequence

>MS.gene004189

ATGATTTCCAAGGGTAGCTGTAAGAAAGCATCACACAGGCTTATCAAGGACAAGGCAAAGAACCATGTAGATGATCTGCATGTGATGTTCCTTGATCTGCAGTTTGCTAGGAAGGAGAGCCGTAGTGTGGATGTCGCGCTTCTTGAGGAGCAAGTTCACCAGATGCTTCGTGAGTGGAAGGCCGAGCTCAATGAGCCTTCTCCTGCTTCTTCTTTGCAGCAGGTAAGCTCAGAATAGTTCTCCTTAACTTTAATATGCAGATTTAATTGTGGATTTGTGATGGACACATGTAAACTTGAATGGCAATATTTGTGGCAAAAACAATGATTTGAGTTTGCCATGTTTATGGGTGATGATAGGGTGGCAGTCTTCGATCATTCTCGACCGATATATGTAGGCTACTGCAGCTCTGTGAAGAGGAAGATGATGCCACTAGTCCATTAGCTGTGCCTAAGCATGAACCTAATGATCAGAATATGCAGACTGAAGCTAAGATGACGTTTCAAGAAGTCTGTTCCTTTCTCCTCTTCAGCGATTATTCATACATCTTTGTGATTTGATCATAACTGGCATAGTTTTTATTTTTTCTGTAGTATTGGCAGTCTAAAATATTGATTTGAAGCTTCTTTTTCTTTATTGCATTATTTTAATTCATATACTAGAGTAAGGGATGCTTTGGAATGTATGATATCTTCTTTTAATGCAAACAGAAAAATATTATAAAGTGTTGTATTGTTTTTTATATGTGGTAGCCTTTTCTCTAAAAAATAAAATTTAATCTTTTATTTTCAATTGACAGAAATGGAGAGCAAGGATTTGATGGTTCAGTCTTTCCTTAGTTAATACCAATTTTCTCACCAACAACTCAAAGCCCAATTTGTTCTTATGCATCATAGTTTTAATCATTATTTCAATGCTTGTCAATATTTTGATCTCACATATGATTGTAACAAGTTTCTTGGTTTAAATTTAAACCAGAGCCAACATCAGCATGATTCCCTATTAATGGATGAGTACATACACTCCATCTTAGGAGCTCCCAGTAAATCAACTAACAACTCGGATAGGCGGCCTAATTTTGAATCAAAATGTCGTCAATTTGATTTAAATGAAGATTTTAATCACAACTGTTATACTGCTTTCAGAGGCACAGGCTTTAGTGAGGATGGTGTTGTTCCTTATATACCTACCTATCTACCAAGCATCTGCCCTCCTCCTTCCGCTTTCCTAGGTTCAAAATGTGCACTTTGGGACTGTTCAAGACCCGCGGAAGGGTTGAACTTGTGTCAAGACTATTGCAGTAGTTTTCATGCTGCTCTTGCTTTAAATGAAGGACAACCAGGCATAGCCCCAGTTCTACGACCTGGGGGAATTGAAGTTAAGGATAATTTACTGTTGGCTGCTCTTAGTGCAAAGACAGAAGGAAAAGATGTCGGCATCCCAGAATGCGAGGGAGCGGCAACTGCAAAGTCTCCGTGGAATGCACCTGGTATTAGAATGTAGAATGCTGAGTTTACCTATTAAACTATTTATGTGTTTATTGAGTATAAAAGTTGAGAAGAGTGTTCTTTTTTGTTGCAGAGCTCTTTGATCTTTTTGTTCTTGAGGGGGAGACTATTAGGGAGTGGCTTTTCTTTGATAAGTCTCGAAGAGCATTTGAGAGTGGAAACAGAAAGCAAAGGTCGTTGCCTGATTATAATGGGCGGGGATGGCATGAATCCAGAAAGCAAGTGATGAAAGAGTTTGGAGGGCTGAAGAGATCCTACTACATGGATCCACAACCACTAAACCATTTTGAGTGGCATCTTTATGAGTATGAAATTAGCAAGTGTGATGCATGTGCGTTATATCGGTTGGAACTAAAGCTCGTTAATGGGAAGAAGAGCTCCAGGACAAAGATAACAAATGATTCAGTTACTGATCTTCAGAAGCGGATAAGAAGACTCTCTGCCGAGTTTCCCTATGATAACAGACATTCTGCTAAGGGCAGAGCCAAACCTAATGCCAAGGCTGACATGGGTGGTGTTTATACAGCTTCAAACAATGCACCATCACCATATGACTATCTTGTTGAGAGCACAATCGCTATTATGTGA
Protein sequence

>MS.gene004189.t1

MISKGSCKKASHRLIKDKAKNHVDDLHVMFLDLQFARKESRSVDVALLEEQVHQMLREWKAELNEPSPASSLQQGGSLRSFSTDICRLLQLCEEEDDATSPLAVPKHEPNDQNMQTEAKMTFQESQHQHDSLLMDEYIHSILGAPSKSTNNSDRRPNFESKCRQFDLNEDFNHNCYTAFRGTGFSEDGVVPYIPTYLPSICPPPSAFLGSKCALWDCSRPAEGLNLCQDYCSSFHAALALNEGQPGIAPVLRPGGIEVKDNLLLAALSAKTEGKDVGIPECEGAATAKSPWNAPELFDLFVLEGETIREWLFFDKSRRAFESGNRKQRSLPDYNGRGWHESRKQVMKEFGGLKRSYYMDPQPLNHFEWHLYEYEISKCDACALYRLELKLVNGKKSSRTKITNDSVTDLQKRIRRLSAEFPYDNRHSAKGRAKPNAKADMGGVYTASNNAPSPYDYLVESTIAIM