AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004200.t1 MTR_2g015000 97.596 416 10 0 1 416 1 416 0.0 832
MS.gene004200.t1 MTR_7g025010 44.622 437 177 18 1 405 1 404 5.31e-87 276
MS.gene004200.t1 MTR_4g088070 39.633 381 189 10 42 405 16 372 7.31e-62 206
MS.gene004200.t1 MTR_4g088080 39.146 281 130 12 46 314 31 282 2.68e-34 130
MS.gene004200.t1 MTR_6g027390 66.667 60 20 0 47 106 24 83 6.17e-23 98.6
MS.gene004200.t1 MTR_7g086940 59.341 91 36 1 39 129 13 102 3.05e-22 95.9
MS.gene004200.t1 MTR_7g086940 46.269 67 36 0 341 407 201 267 1.51e-14 73.9
MS.gene004200.t1 MTR_4g084550 37.805 82 51 0 50 131 59 140 5.11e-18 82.0
MS.gene004200.t1 MTR_4g088065 42.400 125 52 3 64 188 134 238 6.67e-16 77.4
MS.gene004200.t1 MTR_8g107210 35.238 105 68 0 55 159 96 200 1.61e-15 77.8
MS.gene004200.t1 MTR_3g462480 42.708 96 46 1 311 406 4 90 3.81e-15 71.2
MS.gene004200.t1 MTR_1g115315 44.615 65 36 0 53 117 83 147 5.85e-15 75.1
MS.gene004200.t1 MTR_5g081990 45.312 64 35 0 55 118 33 96 6.27e-15 73.2
MS.gene004200.t1 MTR_1g115315 44.615 65 36 0 53 117 83 147 6.31e-15 74.7
MS.gene004200.t1 MTR_3g115620 37.363 91 56 1 24 114 52 141 6.56e-15 73.9
MS.gene004200.t1 MTR_7g060630 43.836 73 41 0 49 121 2 74 2.00e-14 72.0
MS.gene004200.t1 MTR_1g019130 50.000 60 30 0 55 114 90 149 3.85e-14 72.0
MS.gene004200.t1 MTR_5g021930 42.667 75 42 1 49 122 24 98 4.14e-14 73.2
MS.gene004200.t1 MTR_4g063735 50.000 60 30 0 55 114 25 84 7.02e-14 71.6
MS.gene004200.t1 MTR_1g019130 50.000 60 30 0 55 114 90 149 1.09e-13 70.9
MS.gene004200.t1 MTR_7g023810 42.424 66 38 0 49 114 62 127 2.05e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004200.t1 AT5G45980 36.451 417 162 11 1 406 1 325 3.82e-74 234
MS.gene004200.t1 AT2G33880 85.714 126 14 3 1 124 1 124 1.93e-68 221
MS.gene004200.t1 AT2G33880 85.714 126 14 3 1 124 1 124 2.12e-68 222
MS.gene004200.t1 AT3G03660 64.286 70 24 1 38 106 15 84 1.56e-23 99.8
MS.gene004200.t1 AT3G03660 52.632 57 27 0 350 406 208 264 4.46e-13 69.3
MS.gene004200.t1 AT3G03660 64.286 70 24 1 38 106 15 84 2.39e-23 99.8
MS.gene004200.t1 AT3G03660 52.632 57 27 0 350 406 237 293 7.21e-13 69.3
MS.gene004200.t1 AT3G03660 64.286 70 24 1 38 106 15 84 4.31e-23 99.0
MS.gene004200.t1 AT3G03660 53.571 56 26 0 351 406 209 264 1.51e-12 68.2
MS.gene004200.t1 AT3G03660 64.286 70 24 1 38 106 15 84 6.51e-23 99.0
MS.gene004200.t1 AT3G03660 53.571 56 26 0 351 406 238 293 2.04e-12 68.2
MS.gene004200.t1 AT5G17810 67.797 59 18 1 49 106 14 72 3.50e-21 92.8
MS.gene004200.t1 AT5G17810 50.877 57 28 0 350 406 207 263 1.83e-13 70.5
MS.gene004200.t1 AT5G17810 67.797 59 18 1 49 106 14 72 3.64e-21 92.8
MS.gene004200.t1 AT5G17810 50.877 57 28 0 350 406 208 264 1.76e-13 70.9
MS.gene004200.t1 AT5G17810 67.797 59 18 1 49 106 14 72 3.64e-21 92.8
MS.gene004200.t1 AT5G17810 50.877 57 28 0 350 406 208 264 1.76e-13 70.9
MS.gene004200.t1 AT5G05770 34.066 91 60 0 55 145 29 119 1.12e-15 73.6
MS.gene004200.t1 AT3G18010 48.387 62 32 0 55 116 76 137 5.34e-15 76.3
MS.gene004200.t1 AT2G28610 48.529 68 35 0 49 116 2 69 8.65e-15 73.9
MS.gene004200.t1 AT1G20700 46.774 62 33 0 53 114 31 92 1.21e-14 71.2
MS.gene004200.t1 AT3G11260 45.312 64 35 0 55 118 24 87 1.39e-14 72.0
MS.gene004200.t1 AT1G20700 46.774 62 33 0 53 114 93 154 2.55e-14 72.0
MS.gene004200.t1 AT1G20700 46.774 62 33 0 53 114 93 154 2.62e-14 72.0
MS.gene004200.t1 AT1G46480 50.000 60 30 0 55 114 90 149 3.68e-14 71.2
MS.gene004200.t1 AT4G35550 45.161 62 34 0 53 114 97 158 1.34e-13 70.9
MS.gene004200.t1 AT1G46480 50.000 60 30 0 55 114 90 149 1.81e-13 70.5
MS.gene004200.t1 AT2G01500 50.909 55 27 0 55 109 61 115 5.68e-13 69.3
MS.gene004200.t1 AT5G46010 47.619 63 32 1 51 112 50 112 1.07e-12 64.7
MS.gene004200.t1 AT5G59340 43.333 60 34 0 55 114 14 73 1.31e-12 68.2
MS.gene004200.t1 AT2G17950 46.032 63 33 1 55 116 38 100 2.25e-12 67.8

Find 99 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGATTCTGATTCTGATTC+TGG 0.081403 2.4:+69885673 None:intergenic
GAAATTTGAGGAAGTGAATT+AGG 0.138466 2.4:+69885630 None:intergenic
TGCTTCTTGATTTGCGGTTT+TGG 0.159712 2.4:+69885739 None:intergenic
TCTGATTCTTGTTCTGGTTT+TGG 0.210118 2.4:+69885697 None:intergenic
GTGGCCTTTGAGGTTGCTTC+TGG 0.210381 2.4:-69884389 MS.gene004200:CDS
TCTGGTTCTGATTCTTGTTC+TGG 0.215529 2.4:+69885691 None:intergenic
CTTGAAGCTATCTTCAATTC+TGG 0.223036 2.4:-69885855 MS.gene004200:CDS
TTTAATGTTCGTGCGGCATT+TGG 0.234206 2.4:-69884362 MS.gene004200:CDS
ATGCGGATTTGTTCTGGTTT+TGG 0.247339 2.4:+69885879 None:intergenic
TTGGCAGGTATTAATTCTTT+TGG 0.251759 2.4:+69885561 None:intergenic
TTAATGTTCGTGCGGCATTT+GGG 0.270781 2.4:-69884361 MS.gene004200:CDS
CCTTGTGATGTAACATTGTT+AGG 0.298506 2.4:+69885348 None:intergenic
TTTGGATTCCACCTTGGCTT+TGG 0.309423 2.4:+69885897 None:intergenic
CCATTGGTGATGAGTGTTGC+AGG 0.323664 2.4:+69886158 None:intergenic
TCAAGTATGCGGATTTGTTC+TGG 0.334510 2.4:+69885873 None:intergenic
AATGCCGCACGAACATTAAA+AGG 0.340006 2.4:+69884365 None:intergenic
GGCACAACATCATTGATGTT+AGG 0.346091 2.4:+69885519 None:intergenic
GCTGAAAATATGTTTGGTTT+TGG 0.348978 2.4:+69885472 None:intergenic
ATCTTCCTTATCTCTTCTCT+TGG 0.351542 2.4:+69885819 None:intergenic
CTGTTCTTGATCTTTCTTTG+AGG 0.356529 2.4:+69885200 None:intergenic
ACAGAAGCAGTTGGAGAATT+AGG 0.358074 2.4:+69885498 None:intergenic
TTTGAGGCTGAAAATATGTT+TGG 0.371353 2.4:+69885466 None:intergenic
TTCCACCTTGGCTTTGGCTC+AGG 0.373630 2.4:+69885903 None:intergenic
TTCCTTATCTCTTCTCTTGG+TGG 0.377528 2.4:+69885822 None:intergenic
AAATTTGAGGAAGTGAATTA+GGG 0.386654 2.4:+69885631 None:intergenic
CATCAATGATGTGGCCTTTG+AGG 0.391103 2.4:-69884399 MS.gene004200:CDS
CCTTGCACTTGAGAAATGAT+AGG 0.417012 2.4:+69885057 None:intergenic
GTAGGATGTAAAACAGTAGT+AGG 0.421080 2.4:+69885108 None:intergenic
CCAGAATCAGAATCAGAATC+AGG 0.428113 2.4:-69885673 MS.gene004200:CDS
TATGCTTGCTTCTTGATTTG+CGG 0.428894 2.4:+69885733 None:intergenic
GCACAACATCATTGATGTTA+GGG 0.443675 2.4:+69885520 None:intergenic
AGAGCACAGTTGCAAGAGTA+TGG 0.448490 2.4:-69885795 MS.gene004200:CDS
TAGTGCAATTACCAGTGTTA+TGG 0.456754 2.4:+69885268 None:intergenic
GGCTTAGACTTAAACATGCT+TGG 0.459758 2.4:+69886180 None:intergenic
TTACTTGTGGTGGTGGAAGA+AGG 0.462598 2.4:+69885433 None:intergenic
TTCTTCCCAGTGCAACATCA+TGG 0.463536 2.4:-69885387 MS.gene004200:CDS
CTCAAAGAAAGATCAAGAAC+AGG 0.467360 2.4:-69885199 MS.gene004200:CDS
AGATAATTACCTGAGGAGTA+AGG 0.470017 2.4:+69886087 None:intergenic
ATCTCAGGCATTGGTGAACC+AGG 0.475607 2.4:-69884446 MS.gene004200:intron
GCTGTTCTTGTACATCAAAC+TGG 0.484452 2.4:-69884332 MS.gene004200:CDS
GAAAATAACATGCACCATGC+TGG 0.488247 2.4:+69884264 None:intergenic
CCTAACAATGTTACATCACA+AGG 0.488908 2.4:-69885348 MS.gene004200:CDS
CCAAGGGAAAATACTTTGGC+AGG 0.496441 2.4:+69885546 None:intergenic
CCTTGTCCATGATGTTGCAC+TGG 0.500743 2.4:+69885381 None:intergenic
GAAACCAAGGGAAAATACTT+TGG 0.514758 2.4:+69885542 None:intergenic
GATAATTACCTGAGGAGTAA+GGG 0.515469 2.4:+69886088 None:intergenic
CACAGAACCCCTTACTCCTC+AGG 0.516334 2.4:-69886096 MS.gene004200:intron
AGTGAAGAGTGACACCATAC+TGG 0.516391 2.4:+69884291 None:intergenic
TGTTCATGATCTCACTAAGC+AGG 0.516785 2.4:+69885238 None:intergenic
GTGATGGTGGAGGAAGTAGT+AGG 0.517817 2.4:+69885090 None:intergenic
TAATGTTCGTGCGGCATTTG+GGG 0.529446 2.4:-69884360 MS.gene004200:CDS
AAGAAGAAAGTCTCAGTAGC+TGG 0.533457 2.4:+69885405 None:intergenic
GGTACAGTAGCAGAAATTTG+AGG 0.535619 2.4:+69885618 None:intergenic
GGTGCAGTTACTTGTGGTGG+TGG 0.536505 2.4:+69885426 None:intergenic
ATCCACCAAGAGAAGAGATA+AGG 0.537259 2.4:-69885824 MS.gene004200:CDS
TTGCAAGAGTATGGTCAAGT+TGG 0.545746 2.4:-69885786 MS.gene004200:CDS
TACTTGTGGTGGTGGAAGAA+GGG 0.555078 2.4:+69885434 None:intergenic
AGCTATCTTCAATTCTGGAA+TGG 0.556620 2.4:-69885850 MS.gene004200:CDS
GCAAACAGATGTTCAGATGT+GGG 0.556845 2.4:+69885145 None:intergenic
GAGGAAGATGAAGAAGATGA+AGG 0.560821 2.4:+69885597 None:intergenic
CCTGTGTTAACAAACCAGTA+TGG 0.574262 2.4:-69884305 MS.gene004200:CDS
AGGTGATTGAACAGTGATGG+TGG 0.575194 2.4:+69885077 None:intergenic
GGCAAACAGATGTTCAGATG+TGG 0.578783 2.4:+69885144 None:intergenic
AACAGTAGTAGGAGTTGTGG+CGG 0.580421 2.4:+69885119 None:intergenic
CTTGTCCATGATGTTGCACT+GGG 0.582831 2.4:+69885382 None:intergenic
CATTGATGTTAGGGAAACCA+AGG 0.582855 2.4:+69885529 None:intergenic
ACTCTTCACTCACTCCAGCA+TGG 0.587267 2.4:-69884278 MS.gene004200:CDS
CATTGGTGATGAGTGTTGCA+GGG 0.588949 2.4:+69886159 None:intergenic
GTAGCTGGTGCAGTTACTTG+TGG 0.590783 2.4:+69885420 None:intergenic
AGTTGATGTCATGCTGCCAT+TGG 0.593818 2.4:+69886142 None:intergenic
GAAGTCCTGAGCCAAAGCCA+AGG 0.598879 2.4:-69885908 MS.gene004200:CDS
AAACAGATGTTCAGATGTGG+GGG 0.599793 2.4:+69885147 None:intergenic
CCTATCATTTCTCAAGTGCA+AGG 0.604894 2.4:-69885057 MS.gene004200:intron
TGAAGATAGCTTCAAGTATG+CGG 0.609197 2.4:+69885862 None:intergenic
ATAATTACCTGAGGAGTAAG+GGG 0.610710 2.4:+69886089 None:intergenic
TGCTGACCATGTGATTGAGC+AGG 0.611105 2.4:+69885294 None:intergenic
CAAACAGATGTTCAGATGTG+GGG 0.614296 2.4:+69885146 None:intergenic
TAAAACAGTAGTAGGAGTTG+TGG 0.614312 2.4:+69885116 None:intergenic
CCATACTGGTTTGTTAACAC+AGG 0.617631 2.4:+69884305 None:intergenic
GCTGGTGCAGTTACTTGTGG+TGG 0.617972 2.4:+69885423 None:intergenic
AGGTCCAGAAGCAACCTCAA+AGG 0.623758 2.4:+69884385 None:intergenic
ATGGTTCCTGCTCAATCACA+TGG 0.625439 2.4:-69885300 MS.gene004200:CDS
GTAGTAGGAGTTGTGGCGGG+TGG 0.627094 2.4:+69885123 None:intergenic
TGGCTTCATCAAATAGACAC+TGG 0.631121 2.4:-69886203 MS.gene004200:CDS
CCAGTGCAACATCATGGACA+AGG 0.635853 2.4:-69885381 MS.gene004200:CDS
GATAGGTGATTGAACAGTGA+TGG 0.637240 2.4:+69885074 None:intergenic
ACAGTAGTAGGAGTTGTGGC+GGG 0.646909 2.4:+69885120 None:intergenic
GTCCTGAGCCAAAGCCAAGG+TGG 0.648407 2.4:-69885905 MS.gene004200:CDS
CCTGCAACACTCATCACCAA+TGG 0.655110 2.4:-69886158 MS.gene004200:CDS
TGATTGAGCAGGAACCATGT+TGG 0.662612 2.4:+69885305 None:intergenic
ATTGATGTTAGGGAAACCAA+GGG 0.665295 2.4:+69885530 None:intergenic
CTAACAATGTTACATCACAA+GGG 0.668058 2.4:-69885347 MS.gene004200:CDS
GGTCAGCAACACCATAACAC+TGG 0.670176 2.4:-69885279 MS.gene004200:CDS
AGTGAGATCATGAACAACCA+TGG 0.690840 2.4:-69885231 MS.gene004200:CDS
AGTGGTGTTCATCAATGATG+TGG 0.696660 2.4:-69884408 MS.gene004200:CDS
TCTTTGAGGAATTAACACCA+TGG 0.698293 2.4:+69885214 None:intergenic
TGATTGAACAGTGATGGTGG+AGG 0.713068 2.4:+69885080 None:intergenic
AACAGATGTTCAGATGTGGG+GGG 0.713218 2.4:+69885148 None:intergenic
AGGTGTTGCTGCAAAATCAG+TGG 0.717993 2.4:-69884426 MS.gene004200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAGAAACTAAATAATTT+GGG + chr2.4:69885403-69885422 None:intergenic 10.0%
!! AAGTATATAGATGAAATAAT+AGG + chr2.4:69885627-69885646 None:intergenic 15.0%
!! GTTAAAGAAACTAAATAATT+TGG + chr2.4:69885404-69885423 None:intergenic 15.0%
!! TAAAGAAACTAAATAATTTG+GGG + chr2.4:69885402-69885421 None:intergenic 15.0%
!! TATGAAAAATAAATTAGAGA+AGG + chr2.4:69886164-69886183 None:intergenic 15.0%
!!! ATTTTTTTTCTTTTTCTTTG+TGG - chr2.4:69884393-69884412 MS.gene004200:CDS 15.0%
!! AATTTAGAGATGAAAATATG+TGG + chr2.4:69885436-69885455 None:intergenic 20.0%
!! TTGTTACAAATTAGACATTA+GGG + chr2.4:69884361-69884380 None:intergenic 20.0%
!!! CATAGTTTTTGAGATAAAAA+TGG - chr2.4:69885679-69885698 MS.gene004200:CDS 20.0%
!!! TAAAAATGGTAATGTATGTA+TGG - chr2.4:69885693-69885712 MS.gene004200:CDS 20.0%
!!! TTTAGTATTAGTTTGACTTT+TGG - chr2.4:69885820-69885839 MS.gene004200:CDS 20.0%
! AAAATGTAGATAATTACCTG+AGG + chr2.4:69884265-69884284 None:intergenic 25.0%
! AAATTTGAGGAAGTGAATTA+GGG + chr2.4:69884714-69884733 None:intergenic 25.0%
! ATCTCAAAAACTATGTTCTT+AGG + chr2.4:69885674-69885693 None:intergenic 25.0%
! CTTGTTACAAATTAGACATT+AGG + chr2.4:69884362-69884381 None:intergenic 25.0%
!! TTCTTTTTCTTTGTGGAAAT+AGG - chr2.4:69884400-69884419 MS.gene004200:CDS 25.0%
!!! ATATTTTGTTTTGCAGATGT+AGG - chr2.4:69886183-69886202 MS.gene004200:CDS 25.0%
!!! TATTTTGTTTTGCAGATGTA+GGG - chr2.4:69886184-69886203 MS.gene004200:CDS 25.0%
ACTATGTTCTTAGGTTCTAT+AGG + chr2.4:69885665-69885684 None:intergenic 30.0%
AGATTAATGTGAATAAGACG+TGG - chr2.4:69885739-69885758 MS.gene004200:CDS 30.0%
ATAAACTAGTCAAGATCTTG+TGG + chr2.4:69885579-69885598 None:intergenic 30.0%
CTAACAATGTTACATCACAA+GGG - chr2.4:69884995-69885014 MS.gene004200:intron 30.0%
GAAATTTGAGGAAGTGAATT+AGG + chr2.4:69884715-69884734 None:intergenic 30.0%
TATGGAAAAGAAGAGAAAGT+GGG - chr2.4:69885711-69885730 MS.gene004200:CDS 30.0%
! GTGATGCTAATGTTTTCTAT+TGG - chr2.4:69884578-69884597 MS.gene004200:intron 30.0%
! TTGGCAGGTATTAATTCTTT+TGG + chr2.4:69884784-69884803 None:intergenic 30.0%
! TTTGAGGCTGAAAATATGTT+TGG + chr2.4:69884879-69884898 None:intergenic 30.0%
!! ACTTTTGGTAGCTATAGTTA+AGG - chr2.4:69885835-69885854 MS.gene004200:CDS 30.0%
!! GCTGAAAATATGTTTGGTTT+TGG + chr2.4:69884873-69884892 None:intergenic 30.0%
!!! ATTTTGTTTTGCAGATGTAG+GGG - chr2.4:69886185-69886204 MS.gene004200:CDS 30.0%
AGATAATTACCTGAGGAGTA+AGG + chr2.4:69884258-69884277 None:intergenic 35.0%
ATAATTACCTGAGGAGTAAG+GGG + chr2.4:69884256-69884275 None:intergenic 35.0%
ATCTTCCTTATCTCTTCTCT+TGG + chr2.4:69884526-69884545 None:intergenic 35.0%
ATGTTCTTAGGTTCTATAGG+CGG + chr2.4:69885662-69885681 None:intergenic 35.0%
ATTGATGTTAGGGAAACCAA+GGG + chr2.4:69884815-69884834 None:intergenic 35.0%
CCTAACAATGTTACATCACA+AGG - chr2.4:69884994-69885013 MS.gene004200:intron 35.0%
CCTTGTGATGTAACATTGTT+AGG + chr2.4:69884997-69885016 None:intergenic 35.0%
CTCAAAGAAAGATCAAGAAC+AGG - chr2.4:69885143-69885162 MS.gene004200:CDS 35.0%
CTGTTCTTGATCTTTCTTTG+AGG + chr2.4:69885145-69885164 None:intergenic 35.0%
CTTGAAGCTATCTTCAATTC+TGG - chr2.4:69884487-69884506 MS.gene004200:intron 35.0%
GAAACCAAGGGAAAATACTT+TGG + chr2.4:69884803-69884822 None:intergenic 35.0%
GAAATGATTGAGTGTATCTC+AGG - chr2.4:69885881-69885900 MS.gene004200:CDS 35.0%
GATAATTACCTGAGGAGTAA+GGG + chr2.4:69884257-69884276 None:intergenic 35.0%
GCACAACATCATTGATGTTA+GGG + chr2.4:69884825-69884844 None:intergenic 35.0%
GTAGGATGTAAAACAGTAGT+AGG + chr2.4:69885237-69885256 None:intergenic 35.0%
GTATGGAAAAGAAGAGAAAG+TGG - chr2.4:69885710-69885729 MS.gene004200:CDS 35.0%
TAAAACAGTAGTAGGAGTTG+TGG + chr2.4:69885229-69885248 None:intergenic 35.0%
TAGTGCAATTACCAGTGTTA+TGG + chr2.4:69885077-69885096 None:intergenic 35.0%
TGAAGATAGCTTCAAGTATG+CGG + chr2.4:69884483-69884502 None:intergenic 35.0%
! AGAAGGGTAGTTACATTTTG+AGG + chr2.4:69884895-69884914 None:intergenic 35.0%
! AGCTATCTTCAATTCTGGAA+TGG - chr2.4:69884492-69884511 MS.gene004200:intron 35.0%
! CTTTTCTGAACTTTCCAACA+TGG - chr2.4:69885023-69885042 MS.gene004200:intron 35.0%
! GCAGGTATTAATTCTTTTGG+TGG + chr2.4:69884781-69884800 None:intergenic 35.0%
! GTTAGTGTACCATGGTTTTT+GGG - chr2.4:69885761-69885780 MS.gene004200:CDS 35.0%
! TATGCTTGCTTCTTGATTTG+CGG + chr2.4:69884612-69884631 None:intergenic 35.0%
! TCTTTGAGGAATTAACACCA+TGG + chr2.4:69885131-69885150 None:intergenic 35.0%
!! GGCTCTATTGGCTTAAATAT+AGG - chr2.4:69885796-69885815 MS.gene004200:CDS 35.0%
!!! AGTGAATTAGGGTTTTGTTG+TGG + chr2.4:69884703-69884722 None:intergenic 35.0%
!!! TCTGATTCTTGTTCTGGTTT+TGG + chr2.4:69884648-69884667 None:intergenic 35.0%
AAACAGATGTTCAGATGTGG+GGG + chr2.4:69885198-69885217 None:intergenic 40.0%
AAGAAGAAAGTCTCAGTAGC+TGG + chr2.4:69884940-69884959 None:intergenic 40.0%
AATGCCGCACGAACATTAAA+AGG + chr2.4:69885980-69885999 None:intergenic 40.0%
ACAGAAGCAGTTGGAGAATT+AGG + chr2.4:69884847-69884866 None:intergenic 40.0%
AGATGTAGGGGAAAAAATGC+TGG - chr2.4:69886197-69886216 MS.gene004200:CDS 40.0%
AGTGAGATCATGAACAACCA+TGG - chr2.4:69885111-69885130 MS.gene004200:CDS 40.0%
AGTGGTGTTCATCAATGATG+TGG - chr2.4:69885934-69885953 MS.gene004200:intron 40.0%
ATCCACCAAGAGAAGAGATA+AGG - chr2.4:69884518-69884537 MS.gene004200:intron 40.0%
ATTGAGTGTATCTCAGGCAT+TGG - chr2.4:69885887-69885906 MS.gene004200:CDS 40.0%
CAAACAGATGTTCAGATGTG+GGG + chr2.4:69885199-69885218 None:intergenic 40.0%
CATTGATGTTAGGGAAACCA+AGG + chr2.4:69884816-69884835 None:intergenic 40.0%
CCAGAATCAGAATCAGAATC+AGG - chr2.4:69884669-69884688 MS.gene004200:intron 40.0%
CCTATCATTTCTCAAGTGCA+AGG - chr2.4:69885285-69885304 MS.gene004200:CDS 40.0%
CCTGATTCTGATTCTGATTC+TGG + chr2.4:69884672-69884691 None:intergenic 40.0%
CCTGTGTTAACAAACCAGTA+TGG - chr2.4:69886037-69886056 MS.gene004200:intron 40.0%
CCTTGCACTTGAGAAATGAT+AGG + chr2.4:69885288-69885307 None:intergenic 40.0%
GAAAATAACATGCACCATGC+TGG + chr2.4:69886081-69886100 None:intergenic 40.0%
GAGGAAGATGAAGAAGATGA+AGG + chr2.4:69884748-69884767 None:intergenic 40.0%
GATAGGTGATTGAACAGTGA+TGG + chr2.4:69885271-69885290 None:intergenic 40.0%
GCAAACAGATGTTCAGATGT+GGG + chr2.4:69885200-69885219 None:intergenic 40.0%
GCTGTTCTTGTACATCAAAC+TGG - chr2.4:69886010-69886029 MS.gene004200:intron 40.0%
GGCACAACATCATTGATGTT+AGG + chr2.4:69884826-69884845 None:intergenic 40.0%
GGCTTAGACTTAAACATGCT+TGG + chr2.4:69884165-69884184 None:intergenic 40.0%
GGTACAGTAGCAGAAATTTG+AGG + chr2.4:69884727-69884746 None:intergenic 40.0%
TGGCTTCATCAAATAGACAC+TGG - chr2.4:69884139-69884158 MS.gene004200:CDS 40.0%
TGTTCATGATCTCACTAAGC+AGG + chr2.4:69885107-69885126 None:intergenic 40.0%
TTAATGTTCGTGCGGCATTT+GGG - chr2.4:69885981-69886000 MS.gene004200:intron 40.0%
TTCCTTATCTCTTCTCTTGG+TGG + chr2.4:69884523-69884542 None:intergenic 40.0%
TTTAATGTTCGTGCGGCATT+TGG - chr2.4:69885980-69885999 MS.gene004200:intron 40.0%
! GGTTAGTGTACCATGGTTTT+TGG - chr2.4:69885760-69885779 MS.gene004200:CDS 40.0%
! TCAAGTATGCGGATTTGTTC+TGG + chr2.4:69884472-69884491 None:intergenic 40.0%
! TGGACCTTTTAATGTTCGTG+CGG - chr2.4:69885973-69885992 MS.gene004200:intron 40.0%
! TTGCAAGAGTATGGTCAAGT+TGG - chr2.4:69884556-69884575 MS.gene004200:intron 40.0%
! TTTTGGTGGAGATTTCTCAG+AGG + chr2.4:69884767-69884786 None:intergenic 40.0%
!! CCATACTGGTTTGTTAACAC+AGG + chr2.4:69886040-69886059 None:intergenic 40.0%
!! TCTGGTTCTGATTCTTGTTC+TGG + chr2.4:69884654-69884673 None:intergenic 40.0%
!!! ATGCGGATTTGTTCTGGTTT+TGG + chr2.4:69884466-69884485 None:intergenic 40.0%
!!! TCTTGTTCTGGTTTTGGAGA+TGG + chr2.4:69884642-69884661 None:intergenic 40.0%
!!! TGCTTCTTGATTTGCGGTTT+TGG + chr2.4:69884606-69884625 None:intergenic 40.0%
!!! TTTTGGTTCACAGAAGCAGT+TGG + chr2.4:69884856-69884875 None:intergenic 40.0%
AACAGATGTTCAGATGTGGG+GGG + chr2.4:69885197-69885216 None:intergenic 45.0%
AACAGTAGTAGGAGTTGTGG+CGG + chr2.4:69885226-69885245 None:intergenic 45.0%
AAGACGTGGTTAGTGTACCA+TGG - chr2.4:69885753-69885772 MS.gene004200:CDS 45.0%
AGAGCACAGTTGCAAGAGTA+TGG - chr2.4:69884547-69884566 MS.gene004200:intron 45.0%
AGGTGATTGAACAGTGATGG+TGG + chr2.4:69885268-69885287 None:intergenic 45.0%
AGTGAAGAGTGACACCATAC+TGG + chr2.4:69886054-69886073 None:intergenic 45.0%
ATGGTTCCTGCTCAATCACA+TGG - chr2.4:69885042-69885061 MS.gene004200:intron 45.0%
CATCAATGATGTGGCCTTTG+AGG - chr2.4:69885943-69885962 MS.gene004200:intron 45.0%
CCAAGGGAAAATACTTTGGC+AGG + chr2.4:69884799-69884818 None:intergenic 45.0%
CTTGTCCATGATGTTGCACT+GGG + chr2.4:69884963-69884982 None:intergenic 45.0%
GGCAAACAGATGTTCAGATG+TGG + chr2.4:69885201-69885220 None:intergenic 45.0%
TAATGTTCGTGCGGCATTTG+GGG - chr2.4:69885982-69886001 MS.gene004200:intron 45.0%
TGATTGAGCAGGAACCATGT+TGG + chr2.4:69885040-69885059 None:intergenic 45.0%
TTCTTCCCAGTGCAACATCA+TGG - chr2.4:69884955-69884974 MS.gene004200:intron 45.0%
TTTGGATTCCACCTTGGCTT+TGG + chr2.4:69884448-69884467 None:intergenic 45.0%
! AGTTGATGTCATGCTGCCAT+TGG + chr2.4:69884203-69884222 None:intergenic 45.0%
!! ACTGATTTTGCAGCAACACC+TGG + chr2.4:69885917-69885936 None:intergenic 45.0%
!! AGGTGTTGCTGCAAAATCAG+TGG - chr2.4:69885916-69885935 MS.gene004200:CDS 45.0%
!! CATTGGTGATGAGTGTTGCA+GGG + chr2.4:69884186-69884205 None:intergenic 45.0%
!! CCTGCCAAAGTATTTTCCCT+TGG - chr2.4:69884796-69884815 MS.gene004200:intron 45.0%
!! TACTTGTGGTGGTGGAAGAA+GGG + chr2.4:69884911-69884930 None:intergenic 45.0%
!! TGATTGAACAGTGATGGTGG+AGG + chr2.4:69885265-69885284 None:intergenic 45.0%
!! TTACTTGTGGTGGTGGAAGA+AGG + chr2.4:69884912-69884931 None:intergenic 45.0%
!!! TCTGGTTTTGGATTCCACCT+TGG + chr2.4:69884454-69884473 None:intergenic 45.0%
!!! TATTTTTTTATTTTTCTTTT+AGG - chr2.4:69885596-69885615 MS.gene004200:CDS 5.0%
ACAGTAGTAGGAGTTGTGGC+GGG + chr2.4:69885225-69885244 None:intergenic 50.0%
ACTCTTCACTCACTCCAGCA+TGG - chr2.4:69886064-69886083 MS.gene004200:intron 50.0%
AGGTCCAGAAGCAACCTCAA+AGG + chr2.4:69885960-69885979 None:intergenic 50.0%
CACACGTGACCCAAAAACCA+TGG + chr2.4:69885773-69885792 None:intergenic 50.0%
CCAGTGCAACATCATGGACA+AGG - chr2.4:69884961-69884980 MS.gene004200:intron 50.0%
CCTGCAACACTCATCACCAA+TGG - chr2.4:69884184-69884203 MS.gene004200:intron 50.0%
CCTTGTCCATGATGTTGCAC+TGG + chr2.4:69884964-69884983 None:intergenic 50.0%
GGTCAGCAACACCATAACAC+TGG - chr2.4:69885063-69885082 MS.gene004200:CDS 50.0%
GTAGCTGGTGCAGTTACTTG+TGG + chr2.4:69884925-69884944 None:intergenic 50.0%
TGCTGACCATGTGATTGAGC+AGG + chr2.4:69885051-69885070 None:intergenic 50.0%
TGTTCAGATGTGGGGGGATT+TGG + chr2.4:69885191-69885210 None:intergenic 50.0%
! TCACGTGTGCATGGCTCTAT+TGG - chr2.4:69885784-69885803 MS.gene004200:CDS 50.0%
!! ATCTCAGGCATTGGTGAACC+AGG - chr2.4:69885896-69885915 MS.gene004200:CDS 50.0%
!! CCATTGGTGATGAGTGTTGC+AGG + chr2.4:69884187-69884206 None:intergenic 50.0%
!! GTGATGGTGGAGGAAGTAGT+AGG + chr2.4:69885255-69885274 None:intergenic 50.0%
!!! GTTTTTGGGTCACGTGTGCA+TGG - chr2.4:69885775-69885794 MS.gene004200:CDS 50.0%
CACAGAACCCCTTACTCCTC+AGG - chr2.4:69884246-69884265 MS.gene004200:intron 55.0%
GAAGTCCTGAGCCAAAGCCA+AGG - chr2.4:69884434-69884453 MS.gene004200:CDS 55.0%
GCTGGTGCAGTTACTTGTGG+TGG + chr2.4:69884922-69884941 None:intergenic 55.0%
GGTGCAGTTACTTGTGGTGG+TGG + chr2.4:69884919-69884938 None:intergenic 55.0%
!! GTGGCCTTTGAGGTTGCTTC+TGG - chr2.4:69885953-69885972 MS.gene004200:intron 55.0%
!! TTCCACCTTGGCTTTGGCTC+AGG + chr2.4:69884442-69884461 None:intergenic 55.0%
GTCCTGAGCCAAAGCCAAGG+TGG - chr2.4:69884437-69884456 MS.gene004200:CDS 60.0%
! GTAGTAGGAGTTGTGGCGGG+TGG + chr2.4:69885222-69885241 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 69884138 69886226 69884138 ID=MS.gene004200
chr2.4 mRNA 69884138 69886226 69884138 ID=MS.gene004200.t1;Parent=MS.gene004200
chr2.4 exon 69886097 69886226 69886097 ID=MS.gene004200.t1.exon1;Parent=MS.gene004200.t1
chr2.4 CDS 69886097 69886226 69886097 ID=cds.MS.gene004200.t1;Parent=MS.gene004200.t1
chr2.4 exon 69885058 69885942 69885058 ID=MS.gene004200.t1.exon2;Parent=MS.gene004200.t1
chr2.4 CDS 69885058 69885942 69885058 ID=cds.MS.gene004200.t1;Parent=MS.gene004200.t1
chr2.4 exon 69884254 69884461 69884254 ID=MS.gene004200.t1.exon3;Parent=MS.gene004200.t1
chr2.4 CDS 69884254 69884461 69884254 ID=cds.MS.gene004200.t1;Parent=MS.gene004200.t1
chr2.4 exon 69884138 69884165 69884138 ID=MS.gene004200.t1.exon4;Parent=MS.gene004200.t1
chr2.4 CDS 69884138 69884165 69884138 ID=cds.MS.gene004200.t1;Parent=MS.gene004200.t1
Gene Sequence

>MS.gene004200

ATGGCTTCATCAAATAGACACTGGCCAAGCATGTTTAAGTCTAAGCCCTGCAACACTCATCACCAATGGCAGCATGACATCAACTCTTCTCTCATCTCATCTGCTTGTCACAGAACCCCTTACTCCTCAGGTAATTATCTACATTTTCAATCTAACTATACTAATATTTGACTATAGAAAAACACTGATTCAACTTTTGTACGTAAAATATAGTTAGAGACCCTAATGTCTAATTTGTAACAAGTTATTATAGACATTTTTTTTCTTTTTCTTTGTGGAAATAGGTTGTGAAGAGAGAAGTCCTGAGCCAAAGCCAAGGTGGAATCCAAAACCAGAACAAATCCGCATACTTGAAGCTATCTTCAATTCTGGAATGGTGAATCCACCAAGAGAAGAGATAAGGAAGATTAGAGCACAGTTGCAAGAGTATGGTCAAGTTGGTGATGCTAATGTTTTCTATTGGTTCCAAAACCGCAAATCAAGAAGCAAGCATAAGCTTCGCCATCTCCAAAACCAGAACAAGAATCAGAACCAGAATCAGAATCAGAATCAGGATCAATCACCACAACAAAACCCTAATTCACTTCCTCAAATTTCTGCTACTGTACCTTCATCTTCTTCATCTTCCTCTGAGAAATCTCCACCAAAAGAATTAATACCTGCCAAAGTATTTTCCCTTGGTTTCCCTAACATCAATGATGTTGTGCCTAATTCTCCAACTGCTTCTGTGAACCAAAACCAAACATATTTTCAGCCTCAAAATGTAACTACCCTTCTTCCACCACCACAAGTAACTGCACCAGCTACTGAGACTTTCTTCTTCCCAGTGCAACATCATGGACAAGGAATAGTACTTCCTAACAATGTTACATCACAAGGGTTTTGCTTTTCTGAACTTTCCAACATGGTTCCTGCTCAATCACATGGTCAGCAACACCATAACACTGGTAATTGCACTACTAGTTTCCTGCTTAGTGAGATCATGAACAACCATGGTGTTAATTCCTCAAAGAAAGATCAAGAACAGGATAAGTCTGTGAAAATAATGCACCAAATCCCCCCACATCTGAACATCTGTTTGCCACCCGCCACAACTCCTACTACTGTTTTACATCCTACTACTTCCTCCACCATCACTGTTCAATCACCTATCATTTCTCAAGTGCAAGGTAATTAATTACATATATAGCATTTATTTACTTATGCAGTTACAGAAAAAGTTGAAATAATGATATATAGACAAAAATAAAATAAAATTTGACCCCAAATTATTTAGTTTCTTTAACACTACAATTCCACATATTTTCATCTCTAAATTTTTGTTGCTATTTTAACATGTATAGTATTCAAAACTTCTATTCTGCATAGTAATTTTTATTGTTTATATATTTTAAGAAAAGTTTTTATATTAACACATCTTGTAAGTGCAAGAGCAAAACCACAAGATCTTGACTAGTTTATTTTTTTATTTTTCTTTTAGGTTACTCCTATTATTTCATCTATATACTTGTTAATTTTACACCGCCTATAGAACCTAAGAACATAGTTTTTGAGATAAAAATGGTAATGTATGTATGGAAAAGAAGAGAAAGTGGGTAATAAGATTAATGTGAATAAGACGTGGTTAGTGTACCATGGTTTTTGGGTCACGTGTGCATGGCTCTATTGGCTTAAATATAGGCTTTAGTATTAGTTTGACTTTTGGTAGCTATAGTTAAGGTTTGTATTTAGACTTGCACATGAGAAATGATTGAGTGTATCTCAGGCATTGGTGAACCAGGTGTTGCTGCAAAATCAGTGGTGTTCATCAATGATGTGGCCTTTGAGGTTGCTTCTGGACCTTTTAATGTTCGTGCGGCATTTGGGGATGATGCTGTTCTTGTACATCAAACTGGTCAGCCTGTGTTAACAAACCAGTATGGTGTCACTCTTCACTCACTCCAGCATGGTGCATGTTATTTTCTTGTAAGCACGTAAACATACATTATTTTATATACTTGTTTAGTTAATCTTATCAAATTAACCTTCTCTAATTTATTTTTCATATATTTTGTTTTGCAGATGTAGGGGAAAAAATGCTGGAGAGTGA
Protein sequence

>MS.gene004200.t1

MASSNRHWPSMFKSKPCNTHHQWQHDINSSLISSACHRTPYSSGCEERSPEPKPRWNPKPEQIRILEAIFNSGMVNPPREEIRKIRAQLQEYGQVGDANVFYWFQNRKSRSKHKLRHLQNQNKNQNQNQNQNQDQSPQQNPNSLPQISATVPSSSSSSSEKSPPKELIPAKVFSLGFPNINDVVPNSPTASVNQNQTYFQPQNVTTLLPPPQVTAPATETFFFPVQHHGQGIVLPNNVTSQGFCFSELSNMVPAQSHGQQHHNTGNCTTSFLLSEIMNNHGVNSSKKDQEQDKSVKIMHQIPPHLNICLPPATTPTTVLHPTTSSTITVQSPIISQVQGIGEPGVAAKSVVFINDVAFEVASGPFNVRAAFGDDAVLVHQTGQPVLTNQYGVTLHSLQHGACYFLVSTCRGKNAGE