AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004306

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004306.t1 MTR_2g017890 78.910 422 49 2 1 422 2 383 0.0 641
MS.gene004306.t1 MTR_8g087540 48.000 325 163 3 1 324 1 320 1.39e-99 303
MS.gene004306.t1 MTR_5g029680 45.405 185 97 2 13 193 31 215 2.54e-52 183
MS.gene004306.t1 MTR_2g043940 51.786 168 81 0 66 233 14 181 2.09e-51 177
MS.gene004306.t1 MTR_4g077970 40.741 189 101 2 12 193 10 194 4.54e-47 168
MS.gene004306.t1 MTR_7g095680 41.989 181 105 0 13 193 48 228 1.50e-44 159
MS.gene004306.t1 MTR_3g104550 40.113 177 103 1 13 189 45 218 1.15e-43 157
MS.gene004306.t1 MTR_2g100680 35.424 271 143 7 6 257 23 280 1.79e-43 158
MS.gene004306.t1 MTR_2g100670 41.206 199 104 3 1 193 14 205 5.97e-43 153
MS.gene004306.t1 MTR_6g043060 61.111 108 42 0 4 111 12 119 1.11e-42 153
MS.gene004306.t1 MTR_2g100670 41.414 198 103 3 1 192 122 312 2.87e-42 154
MS.gene004306.t1 MTR_1g102860 38.830 188 111 1 5 192 31 214 1.21e-41 150
MS.gene004306.t1 MTR_8g105780 44.624 186 99 2 12 193 20 205 2.66e-41 150
MS.gene004306.t1 MTR_1g061170 37.569 181 108 1 12 192 47 222 3.14e-41 152
MS.gene004306.t1 MTR_8g083100 40.930 215 92 7 15 213 23 218 7.65e-41 151
MS.gene004306.t1 MTR_5g017470 42.775 173 69 2 14 156 9 181 3.30e-40 145
MS.gene004306.t1 MTR_6g086805 39.779 181 102 2 12 192 9 182 2.69e-39 144
MS.gene004306.t1 MTR_1g071360 38.298 188 102 2 5 192 23 196 7.14e-39 143
MS.gene004306.t1 MTR_1g071360 38.298 188 102 2 5 192 20 193 1.68e-38 143
MS.gene004306.t1 MTR_5g010680 57.009 107 45 1 2 107 11 117 2.32e-37 139
MS.gene004306.t1 MTR_3g101870 61.000 100 38 1 8 106 17 116 9.97e-36 135
MS.gene004306.t1 MTR_1g100777 54.717 106 47 1 3 107 6 111 6.96e-35 131
MS.gene004306.t1 MTR_5g089170 57.000 100 42 1 8 106 18 117 3.67e-33 125
MS.gene004306.t1 MTR_7g091370 47.619 105 55 0 14 118 22 126 6.11e-32 121
MS.gene004306.t1 MTR_0020s0020 56.522 92 40 0 14 105 33 124 1.11e-31 124
MS.gene004306.t1 MTR_4g088900 38.037 163 85 3 14 164 33 191 3.77e-27 108
MS.gene004306.t1 MTR_4g045760 51.724 116 39 2 289 404 3 101 5.98e-26 101
MS.gene004306.t1 MTR_4g045757 64.789 71 25 0 1 71 1 71 1.89e-25 99.4
MS.gene004306.t1 MTR_5g047190 49.485 97 42 2 22 111 1 97 7.50e-25 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004306.t1 AT4G18880 43.963 323 166 7 2 315 3 319 6.34e-70 226
MS.gene004306.t1 AT5G45710 40.248 323 152 5 1 323 1 282 2.07e-63 207
MS.gene004306.t1 AT5G45710 40.248 323 152 5 1 323 1 282 2.07e-63 207
MS.gene004306.t1 AT4G17750 43.979 191 96 1 13 192 51 241 2.02e-50 177
MS.gene004306.t1 AT1G32330 42.408 191 104 2 8 192 31 221 2.33e-47 169
MS.gene004306.t1 AT1G32330 42.408 191 104 2 8 192 31 221 2.49e-47 169
MS.gene004306.t1 AT2G26150 41.667 180 102 1 13 192 43 219 2.34e-46 163
MS.gene004306.t1 AT2G26150 41.667 180 102 1 13 192 43 219 2.34e-46 163
MS.gene004306.t1 AT3G02990 41.530 183 106 1 12 193 21 203 5.74e-46 165
MS.gene004306.t1 AT4G13980 40.196 204 108 3 15 212 24 219 4.07e-45 162
MS.gene004306.t1 AT5G16820 40.323 186 109 1 10 193 23 208 8.69e-45 162
MS.gene004306.t1 AT5G16820 40.323 186 109 1 10 193 23 208 8.69e-45 162
MS.gene004306.t1 AT5G03720 38.378 185 103 2 12 193 53 229 4.70e-43 156
MS.gene004306.t1 AT5G03720 38.378 185 103 2 12 193 53 229 4.70e-43 156
MS.gene004306.t1 AT3G22830 40.217 184 106 1 13 192 60 243 1.08e-42 155
MS.gene004306.t1 AT5G62020 51.128 133 57 2 1 132 9 134 2.17e-39 143
MS.gene004306.t1 AT3G24520 38.674 181 85 2 12 192 15 169 2.61e-39 144
MS.gene004306.t1 AT1G67970 40.659 182 104 3 12 192 17 195 3.29e-39 145
MS.gene004306.t1 AT5G43840 41.848 184 88 3 11 192 14 180 4.40e-39 142
MS.gene004306.t1 AT3G63350 39.247 186 92 3 14 192 28 199 5.44e-38 139
MS.gene004306.t1 AT3G51910 35.638 188 103 2 5 192 20 189 1.91e-37 137
MS.gene004306.t1 AT1G46264 59.813 107 39 2 1 106 23 126 5.59e-37 138
MS.gene004306.t1 AT4G36990 57.576 99 42 0 14 112 14 112 1.26e-34 130
MS.gene004306.t1 AT5G54070 37.017 181 98 3 12 190 69 235 2.17e-34 130
MS.gene004306.t1 AT2G41690 53.398 103 47 1 13 114 39 141 3.90e-34 127
MS.gene004306.t1 AT4G11660 58.696 92 38 0 14 105 59 150 1.38e-33 129
MS.gene004306.t1 AT5G45710 32.301 226 113 4 98 323 1 186 1.31e-22 96.7
MS.gene004306.t1 AT5G45710 32.301 226 113 4 98 323 1 186 1.31e-22 96.7
MS.gene004306.t1 AT4G18870 48.864 88 45 0 14 101 146 233 7.04e-22 95.5
MS.gene004306.t1 AT4G18870 46.535 101 52 2 1 101 1 99 1.33e-21 94.7
MS.gene004306.t1 AT1G77570 37.778 90 53 2 15 101 27 116 2.73e-15 73.2
MS.gene004306.t1 AT2G26150 29.412 119 81 1 74 192 49 164 5.66e-14 72.4
MS.gene004306.t1 AT2G26150 29.412 119 81 1 74 192 49 164 5.66e-14 72.4

Find 81 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATTATAATAATGAATTT+TGG 0.100021 2.4:+68164852 MS.gene004306:CDS
GTTACGACGGGTTGTTCTTC+TGG 0.216236 2.4:-68164815 None:intergenic
CAACTGATGACATTGTTTCT+TGG 0.248061 2.4:+68163166 MS.gene004306:CDS
AGTTGCATACAAGATGTTAT+TGG 0.278275 2.4:-68164557 None:intergenic
AACTGATGACATTGTTTCTT+GGG 0.286701 2.4:+68163167 MS.gene004306:CDS
TCTTCTGGTTCAACAAAATC+AGG 0.301153 2.4:-68164800 None:intergenic
TAAGGTAATTTAGACTCTTT+TGG 0.303752 2.4:-68164942 None:intergenic
TGAAACTTGAGAAGTTATTA+TGG 0.310950 2.4:-68163263 None:intergenic
TCTCACCAAGACTTATGATA+TGG 0.311693 2.4:+68163131 MS.gene004306:CDS
ATGCTAGCTACATTACAAAA+AGG 0.320103 2.4:-68164332 None:intergenic
GGCTCAGTTGCAACCGGATT+AGG 0.358087 2.4:-68164722 None:intergenic
CGAGTCGAGTTGGCCAATAT+TGG 0.365906 2.4:+68164915 MS.gene004306:CDS
GGCTCAGTTGCATCATGATT+AGG 0.376887 2.4:-68164683 None:intergenic
TTATTATAATCAGTAGTAAC+CGG 0.377060 2.4:-68164842 None:intergenic
CGGATGTCTTTGAAAATAAG+AGG 0.384067 2.4:-68164495 None:intergenic
AAGAAACCTGGGAATGAGTT+AGG 0.397323 2.4:+68164254 MS.gene004306:CDS
ACAGTACTCGAAGGGTCATT+TGG 0.400137 2.4:+68164178 MS.gene004306:CDS
GACTCAGTTGCAACTGGATC+AGG 0.430111 2.4:-68164644 None:intergenic
TGTAATCGTTGTTCCTAATC+CGG 0.434226 2.4:+68164709 MS.gene004306:CDS
ACCGCTGGCTCAGTTGCAAC+CGG 0.439728 2.4:-68164728 None:intergenic
GTTTCTTGAACTTCAGAGAA+AGG 0.439975 2.4:+68164124 MS.gene004306:CDS
GGGAATGATGGAAGAAGCTC+AGG 0.447000 2.4:+68163080 None:intergenic
CATGTCGACTTCTAGTGACT+TGG 0.447575 2.4:-68164597 None:intergenic
GAGAAAAGGAGGCACCTTAT+CGG 0.464277 2.4:-68163112 None:intergenic
TTGAATCATCAATGACAAGT+TGG 0.472901 2.4:+68164447 MS.gene004306:CDS
ACATCCGACTCAGTTGCAAC+TGG 0.474094 2.4:-68164650 None:intergenic
TCATAAGTCTTGGTGAGAAA+AGG 0.480198 2.4:-68163126 None:intergenic
GATGACTCAATGAATTATGT+AGG 0.484303 2.4:+68164530 MS.gene004306:CDS
ACAATACTTGCAGGACGAAT+CGG 0.489021 2.4:+68164877 MS.gene004306:CDS
CTCAACCATATCATAAGTCT+TGG 0.489284 2.4:-68163136 None:intergenic
CATGAGCATTCTGCGAAGAA+TGG 0.490641 2.4:-68164013 None:intergenic
CATATACCTAACTCATTCCC+AGG 0.495219 2.4:-68164260 None:intergenic
GAGTCATCAAAATCTACATT+CGG 0.497020 2.4:-68164515 None:intergenic
TGAACTTCAGAGAAAGGAAG+AGG 0.498686 2.4:+68164130 MS.gene004306:CDS
TCGCAGAATGCTCATGGCCA+AGG 0.501991 2.4:+68164020 MS.gene004306:CDS
GGTCAAGTGCTGAAGAAACC+TGG 0.503718 2.4:+68164242 MS.gene004306:CDS
TTATTATGGTTAAAGTATTG+AGG 0.507346 2.4:-68163249 None:intergenic
CGCAGAATGCTCATGGCCAA+GGG 0.508087 2.4:+68164021 MS.gene004306:CDS
TGAATCATCAATGACAAGTT+GGG 0.509603 2.4:+68164448 MS.gene004306:CDS
GAAACAATGTCATCAGTTGA+AGG 0.519956 2.4:-68163162 None:intergenic
TTAGGAACAACGATTACATT+CGG 0.521576 2.4:-68164704 None:intergenic
CAATATTGGCCAACTCGACT+CGG 0.523538 2.4:-68164914 None:intergenic
CTAGTGGAGCAGCAACCCCT+TGG 0.524076 2.4:-68164037 None:intergenic
TCAGGGTAGTAGTTCCGATA+AGG 0.544906 2.4:+68163098 MS.gene004306:CDS
CATTCTTCGCAGAATGCTCA+TGG 0.549403 2.4:+68164014 MS.gene004306:CDS
TGTCGATCGGATTCTGCTAG+TGG 0.551922 2.4:-68164053 None:intergenic
CAAAGTGTGCTCTCTTATGT+TGG 0.554182 2.4:+68164221 MS.gene004306:CDS
ATGTCGACTTCTAGTGACTT+GGG 0.560272 2.4:-68164596 None:intergenic
CAAGACTTATGATATGGTTG+AGG 0.564774 2.4:+68163137 MS.gene004306:CDS
AGCATTCAATGTCTGTCGAT+CGG 0.568118 2.4:-68164066 None:intergenic
AGCTAGCATTGAAGATCATG+TGG 0.568546 2.4:+68164346 MS.gene004306:CDS
GGATCAGGAACAACTACCAC+TGG 0.574913 2.4:-68164629 None:intergenic
TCGGAAGCATCGATTACCGC+TGG 0.577204 2.4:-68164743 None:intergenic
GATAAAATTACAGTACTCGA+AGG 0.578742 2.4:+68164169 MS.gene004306:CDS
ACTGATGACATTGTTTCTTG+GGG 0.579964 2.4:+68163168 MS.gene004306:CDS
GTCAAGTGCTGAAGAAACCT+GGG 0.583533 2.4:+68164243 MS.gene004306:CDS
TCAGGGACAGCTGCAACAGT+TGG 0.583782 2.4:-68164782 None:intergenic
GAAGATATACCGAGTCGAGT+TGG 0.590581 2.4:+68164905 MS.gene004306:CDS
TATTATAATCAGTAGTAACC+GGG 0.593232 2.4:-68164841 None:intergenic
AGGAACAACTACCACTGGCA+TGG 0.597086 2.4:-68164624 None:intergenic
CTTCTGGTTCAACAAAATCA+GGG 0.603287 2.4:-68164799 None:intergenic
TGATCCAGTTGCAACTGAGT+CGG 0.607702 2.4:+68164646 MS.gene004306:CDS
TAAGTCTTGGTGAGAAAAGG+AGG 0.608589 2.4:-68163123 None:intergenic
TGGCTCAACTACAACAAGAT+CGG 0.609117 2.4:-68164762 None:intergenic
TCCGGTTGCAACTGAGCCAG+CGG 0.609490 2.4:+68164727 MS.gene004306:CDS
GTCATTTGGAAACAGTGGAA+AGG 0.611941 2.4:+68164192 MS.gene004306:CDS
ATCTTATGAAGAATATCCAA+AGG 0.635890 2.4:+68163973 MS.gene004306:CDS
ATAAAATTACAGTACTCGAA+GGG 0.640506 2.4:+68164170 MS.gene004306:CDS
GTAACCGGGGATGTTACGAC+GGG 0.648034 2.4:-68164827 None:intergenic
ACAACCCGTCGTAACATCCC+CGG 0.648155 2.4:+68164823 MS.gene004306:CDS
ATCATCAATGACAAGTTGGG+AGG 0.657482 2.4:+68164451 MS.gene004306:CDS
GTCTCTTCTATCATTAAACG+TGG 0.666811 2.4:+68164409 MS.gene004306:CDS
CGACATGAACTCCATGCCAG+TGG 0.675176 2.4:+68164613 MS.gene004306:CDS
GCTAGCATTGAAGATCATGT+GGG 0.675912 2.4:+68164347 MS.gene004306:CDS
AATGATAGAAGAGACACACT+CGG 0.677326 2.4:-68164401 None:intergenic
ATGATAGAAGAGACACACTC+GGG 0.678625 2.4:-68164400 None:intergenic
AGTAACCGGGGATGTTACGA+CGG 0.681684 2.4:-68164828 None:intergenic
GAAGGGTCATTTGGAAACAG+TGG 0.685082 2.4:+68164187 MS.gene004306:CDS
ATTATAATCAGTAGTAACCG+GGG 0.692233 2.4:-68164840 None:intergenic
GCAGAATGCTCATGGCCAAG+GGG 0.714345 2.4:+68164022 MS.gene004306:CDS
GGAATGATGGAAGAAGCTCA+GGG 0.733848 2.4:+68163081 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTATAATAATGAATTTT+GGG + chr2.4:68164853-68164872 MS.gene004306:CDS 10.0%
!!! TTATTGTTTTTTTATTAAGT+TGG - chr2.4:68163724-68163743 None:intergenic 10.0%
!! TTAAATAAATCAATCAAATC+AGG - chr2.4:68163522-68163541 None:intergenic 15.0%
!!! CTGATTATAATAATGAATTT+TGG + chr2.4:68164852-68164871 MS.gene004306:CDS 15.0%
!!! GATTATAATAATGAATTTTG+GGG + chr2.4:68164854-68164873 MS.gene004306:CDS 15.0%
!! AAAAATTGATACACAACTAT+GGG + chr2.4:68163914-68163933 MS.gene004306:CDS 20.0%
!! TTATTATAATCAGTAGTAAC+CGG - chr2.4:68164845-68164864 None:intergenic 20.0%
!!! TTAAGCTTATGTATTTTCAT+AGG + chr2.4:68163538-68163557 MS.gene004306:intron 20.0%
!!! TTATTATGGTTAAAGTATTG+AGG - chr2.4:68163252-68163271 None:intergenic 20.0%
! AAGCAATAAAAAAACCTGAA+TGG - chr2.4:68163422-68163441 None:intergenic 25.0%
! AAGCAATCAATAAACCTTAA+TGG - chr2.4:68163856-68163875 None:intergenic 25.0%
! AGCAATCAATAAACCTTAAT+GGG - chr2.4:68163855-68163874 None:intergenic 25.0%
! ATAAAAGAACAAAGCTTGAT+TGG - chr2.4:68163679-68163698 None:intergenic 25.0%
! ATAAAATTACAGTACTCGAA+GGG + chr2.4:68164170-68164189 MS.gene004306:CDS 25.0%
! ATCTTATGAAGAATATCCAA+AGG + chr2.4:68163973-68163992 MS.gene004306:CDS 25.0%
! CAAAAACTACAAAGCAAAAA+AGG + chr2.4:68164291-68164310 MS.gene004306:CDS 25.0%
! CTTTAATGTACTTCATTTCA+TGG + chr2.4:68163314-68163333 MS.gene004306:intron 25.0%
! GAAAAATTGATACACAACTA+TGG + chr2.4:68163913-68163932 MS.gene004306:CDS 25.0%
! GAGGAGATTAAAAATTATCT+TGG + chr2.4:68164470-68164489 MS.gene004306:CDS 25.0%
! TATTATAATCAGTAGTAACC+GGG - chr2.4:68164844-68164863 None:intergenic 25.0%
! TGAAACTTGAGAAGTTATTA+TGG - chr2.4:68163266-68163285 None:intergenic 25.0%
!! TTACAAAAAGGATTGTTTCT+TGG - chr2.4:68164323-68164342 None:intergenic 25.0%
!! TTAGACTGTGTTATGTTTTT+CGG + chr2.4:68163440-68163459 MS.gene004306:intron 25.0%
!! TTTTTCTAATCATTCCATTC+AGG + chr2.4:68163405-68163424 MS.gene004306:intron 25.0%
!!! CTGATGAAAGTTTTATTGTT+TGG + chr2.4:68163196-68163215 MS.gene004306:CDS 25.0%
AAACTACAAAGCAAAAAAGG+AGG + chr2.4:68164294-68164313 MS.gene004306:CDS 30.0%
AGTTGCATACAAGATGTTAT+TGG - chr2.4:68164560-68164579 None:intergenic 30.0%
ATGCTAGCTACATTACAAAA+AGG - chr2.4:68164335-68164354 None:intergenic 30.0%
ATTATAATCAGTAGTAACCG+GGG - chr2.4:68164843-68164862 None:intergenic 30.0%
GAGTCATCAAAATCTACATT+CGG - chr2.4:68164518-68164537 None:intergenic 30.0%
GATAAAATTACAGTACTCGA+AGG + chr2.4:68164169-68164188 MS.gene004306:CDS 30.0%
GATGACTCAATGAATTATGT+AGG + chr2.4:68164530-68164549 MS.gene004306:CDS 30.0%
GTTAGAAAAATTGATCATCG+TGG + chr2.4:68163475-68163494 MS.gene004306:intron 30.0%
TGAATCATCAATGACAAGTT+GGG + chr2.4:68164448-68164467 MS.gene004306:CDS 30.0%
TGTGAGATAGCTTATGTTAT+AGG + chr2.4:68163633-68163652 MS.gene004306:intron 30.0%
TTAGGAACAACGATTACATT+CGG - chr2.4:68164707-68164726 None:intergenic 30.0%
TTGAATCATCAATGACAAGT+TGG + chr2.4:68164447-68164466 MS.gene004306:CDS 30.0%
! AACTGATGACATTGTTTCTT+GGG + chr2.4:68163167-68163186 MS.gene004306:CDS 30.0%
! CTTCTTTTTATCACCCATTA+AGG + chr2.4:68163839-68163858 MS.gene004306:intron 30.0%
! TTGAACTGTCTTATGGTTTT+TGG + chr2.4:68163874-68163893 MS.gene004306:intron 30.0%
!!! TATGGTTTTTGGTTTGATCA+GGG + chr2.4:68163885-68163904 MS.gene004306:intron 30.0%
!!! TTATGGTTTTTGGTTTGATC+AGG + chr2.4:68163884-68163903 MS.gene004306:intron 30.0%
AATGATAGAAGAGACACACT+CGG - chr2.4:68164404-68164423 None:intergenic 35.0%
CAAGACTTATGATATGGTTG+AGG + chr2.4:68163137-68163156 MS.gene004306:CDS 35.0%
CAGCTTAATACCTATGTAAG+CGG + chr2.4:68163291-68163310 MS.gene004306:intron 35.0%
CTCAACCATATCATAAGTCT+TGG - chr2.4:68163139-68163158 None:intergenic 35.0%
GAAACAATGTCATCAGTTGA+AGG - chr2.4:68163165-68163184 None:intergenic 35.0%
GTCTCTTCTATCATTAAACG+TGG + chr2.4:68164409-68164428 MS.gene004306:CDS 35.0%
GTTTCTTGAACTTCAGAGAA+AGG + chr2.4:68164124-68164143 MS.gene004306:CDS 35.0%
TACATTAAAGCCGCTTACAT+AGG - chr2.4:68163304-68163323 None:intergenic 35.0%
TCATAAGTCTTGGTGAGAAA+AGG - chr2.4:68163129-68163148 None:intergenic 35.0%
TCTCACCAAGACTTATGATA+TGG + chr2.4:68163131-68163150 MS.gene004306:CDS 35.0%
TGATTGCTTGAACTGTCTTA+TGG + chr2.4:68163867-68163886 MS.gene004306:intron 35.0%
TGTAATCGTTGTTCCTAATC+CGG + chr2.4:68164709-68164728 MS.gene004306:CDS 35.0%
! ACTGATGACATTGTTTCTTG+GGG + chr2.4:68163168-68163187 MS.gene004306:CDS 35.0%
! CAACTGATGACATTGTTTCT+TGG + chr2.4:68163166-68163185 MS.gene004306:CDS 35.0%
! CGGATGTCTTTGAAAATAAG+AGG - chr2.4:68164498-68164517 None:intergenic 35.0%
! TATAAACTGTTTTGCGCCTT+TGG - chr2.4:68163992-68164011 None:intergenic 35.0%
! TGCTTTGTAGTTTTTGTCAG+CGG - chr2.4:68164287-68164306 None:intergenic 35.0%
!! CAGAAGATCAAAGCATTGTT+TGG - chr2.4:68163806-68163825 None:intergenic 35.0%
!! CTTCTGGTTCAACAAAATCA+GGG - chr2.4:68164802-68164821 None:intergenic 35.0%
!! TCTTCTGGTTCAACAAAATC+AGG - chr2.4:68164803-68164822 None:intergenic 35.0%
AAGAAACCTGGGAATGAGTT+AGG + chr2.4:68164254-68164273 MS.gene004306:CDS 40.0%
ACAATACTTGCAGGACGAAT+CGG + chr2.4:68164877-68164896 MS.gene004306:CDS 40.0%
AGCATTCAATGTCTGTCGAT+CGG - chr2.4:68164069-68164088 None:intergenic 40.0%
ATGATAGAAGAGACACACTC+GGG - chr2.4:68164403-68164422 None:intergenic 40.0%
CAAAGTGTGCTCTCTTATGT+TGG + chr2.4:68164221-68164240 MS.gene004306:CDS 40.0%
CATATACCTAACTCATTCCC+AGG - chr2.4:68164263-68164282 None:intergenic 40.0%
GTCATTTGGAAACAGTGGAA+AGG + chr2.4:68164192-68164211 MS.gene004306:CDS 40.0%
TAAGTCTTGGTGAGAAAAGG+AGG - chr2.4:68163126-68163145 None:intergenic 40.0%
TGAACTTCAGAGAAAGGAAG+AGG + chr2.4:68164130-68164149 MS.gene004306:CDS 40.0%
TGGCTCAACTACAACAAGAT+CGG - chr2.4:68164765-68164784 None:intergenic 40.0%
! AGCTAGCATTGAAGATCATG+TGG + chr2.4:68164346-68164365 MS.gene004306:CDS 40.0%
! ATCATCAATGACAAGTTGGG+AGG + chr2.4:68164451-68164470 MS.gene004306:CDS 40.0%
! CTCTTTTGGTACGCCAATAT+TGG - chr2.4:68164931-68164950 None:intergenic 40.0%
! GCTAGCATTGAAGATCATGT+GGG + chr2.4:68164347-68164366 MS.gene004306:CDS 40.0%
!! ATGTCGACTTCTAGTGACTT+GGG - chr2.4:68164599-68164618 None:intergenic 40.0%
!! ATTTTGGGGACAATACTTGC+AGG + chr2.4:68164868-68164887 MS.gene004306:CDS 40.0%
ACAGTACTCGAAGGGTCATT+TGG + chr2.4:68164178-68164197 MS.gene004306:CDS 45.0%
CAATATTGGCCAACTCGACT+CGG - chr2.4:68164917-68164936 None:intergenic 45.0%
CATGAGCATTCTGCGAAGAA+TGG - chr2.4:68164016-68164035 None:intergenic 45.0%
CATTCTTCGCAGAATGCTCA+TGG + chr2.4:68164014-68164033 MS.gene004306:CDS 45.0%
GAAGATATACCGAGTCGAGT+TGG + chr2.4:68164905-68164924 MS.gene004306:CDS 45.0%
GAAGGGTCATTTGGAAACAG+TGG + chr2.4:68164187-68164206 MS.gene004306:CDS 45.0%
GGCTCAGTTGCATCATGATT+AGG - chr2.4:68164686-68164705 None:intergenic 45.0%
TCAGGGTAGTAGTTCCGATA+AGG + chr2.4:68163098-68163117 MS.gene004306:CDS 45.0%
TGATCCAGTTGCAACTGAGT+CGG + chr2.4:68164646-68164665 MS.gene004306:CDS 45.0%
!! CATGTCGACTTCTAGTGACT+TGG - chr2.4:68164600-68164619 None:intergenic 45.0%
!! GAGAAAAGGAGGCACCTTAT+CGG - chr2.4:68163115-68163134 None:intergenic 45.0%
!! GTCAAGTGCTGAAGAAACCT+GGG + chr2.4:68164243-68164262 MS.gene004306:CDS 45.0%
ACATCCGACTCAGTTGCAAC+TGG - chr2.4:68164653-68164672 None:intergenic 50.0%
AGGAACAACTACCACTGGCA+TGG - chr2.4:68164627-68164646 None:intergenic 50.0%
AGTAACCGGGGATGTTACGA+CGG - chr2.4:68164831-68164850 None:intergenic 50.0%
GACTCAGTTGCAACTGGATC+AGG - chr2.4:68164647-68164666 None:intergenic 50.0%
GGATCAGGAACAACTACCAC+TGG - chr2.4:68164632-68164651 None:intergenic 50.0%
TGTCGATCGGATTCTGCTAG+TGG - chr2.4:68164056-68164075 None:intergenic 50.0%
!! CGAGTCGAGTTGGCCAATAT+TGG + chr2.4:68164915-68164934 MS.gene004306:CDS 50.0%
!! GGTCAAGTGCTGAAGAAACC+TGG + chr2.4:68164242-68164261 MS.gene004306:CDS 50.0%
!! GTTACGACGGGTTGTTCTTC+TGG - chr2.4:68164818-68164837 None:intergenic 50.0%
ACAACCCGTCGTAACATCCC+CGG + chr2.4:68164823-68164842 MS.gene004306:CDS 55.0%
CGACATGAACTCCATGCCAG+TGG + chr2.4:68164613-68164632 MS.gene004306:CDS 55.0%
CGCAGAATGCTCATGGCCAA+GGG + chr2.4:68164021-68164040 MS.gene004306:CDS 55.0%
GCAGAATGCTCATGGCCAAG+GGG + chr2.4:68164022-68164041 MS.gene004306:CDS 55.0%
GGCTCAGTTGCAACCGGATT+AGG - chr2.4:68164725-68164744 None:intergenic 55.0%
GTAACCGGGGATGTTACGAC+GGG - chr2.4:68164830-68164849 None:intergenic 55.0%
TCAGGGACAGCTGCAACAGT+TGG - chr2.4:68164785-68164804 None:intergenic 55.0%
TCGCAGAATGCTCATGGCCA+AGG + chr2.4:68164020-68164039 MS.gene004306:CDS 55.0%
!! TCGGAAGCATCGATTACCGC+TGG - chr2.4:68164746-68164765 None:intergenic 55.0%
CTAGTGGAGCAGCAACCCCT+TGG - chr2.4:68164040-68164059 None:intergenic 60.0%
TCCGGTTGCAACTGAGCCAG+CGG + chr2.4:68164727-68164746 MS.gene004306:CDS 60.0%
!! ACCGCTGGCTCAGTTGCAAC+CGG - chr2.4:68164731-68164750 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 68163087 68164955 68163087 ID=MS.gene004306
chr2.4 mRNA 68163087 68164955 68163087 ID=MS.gene004306.t1;Parent=MS.gene004306
chr2.4 exon 68163087 68163305 68163087 ID=MS.gene004306.t1.exon1;Parent=MS.gene004306.t1
chr2.4 CDS 68163087 68163305 68163087 ID=cds.MS.gene004306.t1;Parent=MS.gene004306.t1
chr2.4 exon 68163906 68164955 68163906 ID=MS.gene004306.t1.exon2;Parent=MS.gene004306.t1
chr2.4 CDS 68163906 68164955 68163906 ID=cds.MS.gene004306.t1;Parent=MS.gene004306.t1
Gene Sequence

>MS.gene004306

ATGGAAGAAGCTCAGGGTAGTAGTTCCGATAAGGTGCCTCCTTTTCTCACCAAGACTTATGATATGGTTGAGGATCCTTCAACTGATGACATTGTTTCTTGGGGTGCTACTGATGAAAGTTTTATTGTTTGGAATAAACTAGATTTTGAAAAAGATGTGTTGCCTCAATACTTTAACCATAATAACTTCTCAAGTTTCATCAGACAGCTTAATACCTATGTAAGCGGCTTTAATGTACTTCATTTCATGGATTTTTAAATTGTTTTGTTATTTCGTTAATGTATAATGTTTTGTTATTGTCTTAATGTTGTCTCTTTTTTTTTCTAATCATTCCATTCAGGTTTTTTTATTGCTTAGACTGTGTTATGTTTTTCGGTTTGATTATGTCGTTAGAAAAATTGATCATCGTGGATTTGTTAATTTTTTTATGAGCCTGATTTGATTGATTTATTTAAGCTTATGTATTTTCATAGGCACGCGTAAGACTAATTGAGATAGCTTATAGAAAAACAACTTCATAAGTTGTTCAGTTTATTTTATTTTCATTGTGAGATAGCTTATGTTATAGGCACTTTATTAAGTTGTTGATCCAATCAAGCTTTGTTCTTTTATTGTTACTGTATTTTCATCACTGCCAACTTAATAAAAAAACAATAACAATAACAACAAAAATCATATAAGTGTTTATGTTATAAACACTCAATTAACTTGCTCATCCAAACAATGCTTTGATCTTCTGTTTTTTTCTCTCTCTTCTTTTTATCACCCATTAAGGTTTATTGATTGCTTGAACTGTCTTATGGTTTTTGGTTTGATCAGGGCTTTAGAAAAATTGATACACAACTATGGGAATACGCAAACGATGATTTCGTAAGAAATCAAAAACATCTTATGAAGAATATCCAAAGGCGCAAAACAGTTTATAGCCATTCTTCGCAGAATGCTCATGGCCAAGGGGTTGCTGCTCCACTAGCAGAATCCGATCGACAGACATTGAATGCTCATATTCAAAAACTTAAACACGACAATGAACAAATGTTTCTTGAACTTCAGAGAAAGGAAGAGGAGAGAAAAATGCATGAGATAAAATTACAGTACTCGAAGGGTCATTTGGAAACAGTGGAAAGGAAACATCAAAGTGTGCTCTCTTATGTTGGTCAAGTGCTGAAGAAACCTGGGAATGAGTTAGGTATATGTCCGCTGACAAAAACTACAAAGCAAAAAAGGAGGATTCCAAGAAACAATCCTTTTTGTAATGTAGCTAGCATTGAAGATCATGTGGGAACTTCTCATGTAATACATAAAGAAAACGACCCGAGTGTGTCTCTTCTATCATTAAACGTGGAACAATTAAATTTGCTTGAATCATCAATGACAAGTTGGGAGGAGATTAAAAATTATCTTGGTGCCTCTTATTTTCAAAGACATCCGAATGTAGATTTTGATGACTCAATGAATTATGTAGGCTGTCCAATAACATCTTGTATGCAACTAGATGTTGAAGTTCAGCCCAAGTCACTAGAAGTCGACATGAACTCCATGCCAGTGGTAGTTGTTCCTGATCCAGTTGCAACTGAGTCGGATGTAGTTGTTAATCCTAATCATGATGCAACTGAGCCGAATGTAATCGTTGTTCCTAATCCGGTTGCAACTGAGCCAGCGGTAATCGATGCTTCCGATCTTGTTGTAGTTGAGCCAACTGTTGCAGCTGTCCCTGATTTTGTTGAACCAGAAGAACAACCCGTCGTAACATCCCCGGTTACTACTGATTATAATAATGAATTTTGGGGACAATACTTGCAGGACGAATCGGAAAATGAAGATATACCGAGTCGAGTTGGCCAATATTGGCGTACCAAAAGAGTCTAA
Protein sequence

>MS.gene004306.t1

MEEAQGSSSDKVPPFLTKTYDMVEDPSTDDIVSWGATDESFIVWNKLDFEKDVLPQYFNHNNFSSFIRQLNTYGFRKIDTQLWEYANDDFVRNQKHLMKNIQRRKTVYSHSSQNAHGQGVAAPLAESDRQTLNAHIQKLKHDNEQMFLELQRKEEERKMHEIKLQYSKGHLETVERKHQSVLSYVGQVLKKPGNELGICPLTKTTKQKRRIPRNNPFCNVASIEDHVGTSHVIHKENDPSVSLLSLNVEQLNLLESSMTSWEEIKNYLGASYFQRHPNVDFDDSMNYVGCPITSCMQLDVEVQPKSLEVDMNSMPVVVVPDPVATESDVVVNPNHDATEPNVIVVPNPVATEPAVIDASDLVVVEPTVAAVPDFVEPEEQPVVTSPVTTDYNNEFWGQYLQDESENEDIPSRVGQYWRTKRV