AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004445

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004445.t1 MTR_2g019840 85.171 263 39 0 2 264 123 385 1.88e-163 458
MS.gene004445.t1 MTR_2g019810 75.099 253 63 0 2 254 111 363 4.65e-138 393
MS.gene004445.t1 MTR_2g436400 47.012 251 129 3 2 250 129 377 1.72e-70 221
MS.gene004445.t1 MTR_4g119550 42.529 261 146 3 2 260 132 390 9.10e-68 215
MS.gene004445.t1 MTR_2g437030 44.314 255 138 3 2 254 208 460 9.07e-65 209
MS.gene004445.t1 MTR_2g436440 41.406 256 146 3 2 255 128 381 3.22e-63 203
MS.gene004445.t1 MTR_2g437080 44.141 256 139 3 2 255 122 375 7.64e-61 197
MS.gene004445.t1 MTR_2g437060 43.825 251 137 3 2 250 137 385 6.37e-60 195
MS.gene004445.t1 MTR_2g437040 39.844 256 148 5 2 254 122 374 1.06e-57 189
MS.gene004445.t1 MTR_2g437960 42.471 259 142 5 2 255 135 391 4.31e-57 187
MS.gene004445.t1 MTR_2g437960 42.471 259 142 5 2 255 135 391 5.59e-57 188
MS.gene004445.t1 MTR_2g436460 42.745 255 141 4 2 253 127 379 5.97e-57 186
MS.gene004445.t1 MTR_2g437020 42.692 260 146 3 2 259 121 379 1.15e-56 186
MS.gene004445.t1 MTR_2g437240 40.755 265 138 5 2 255 147 403 6.63e-56 185
MS.gene004445.t1 MTR_2g437990 41.445 263 138 5 2 255 132 387 9.31e-56 185
MS.gene004445.t1 MTR_2g437120 41.923 260 145 4 2 257 133 390 2.52e-55 183
MS.gene004445.t1 MTR_2g438020 42.857 259 141 4 2 255 186 442 7.10e-54 181
MS.gene004445.t1 MTR_2g076320 40.996 261 147 5 2 257 132 390 1.28e-53 179
MS.gene004445.t1 MTR_2g437260 35.985 264 167 2 2 263 116 379 1.92e-53 178
MS.gene004445.t1 MTR_2g438010 39.453 256 148 4 5 255 155 408 3.45e-53 178
MS.gene004445.t1 MTR_2g437160 40.769 260 148 4 2 257 145 402 4.59e-53 177
MS.gene004445.t1 MTR_2g437130 40.078 257 148 5 2 255 123 376 1.21e-52 176
MS.gene004445.t1 MTR_2g437200 41.732 254 141 4 2 250 147 398 2.11e-52 175
MS.gene004445.t1 MTR_2g437100 42.205 263 133 6 2 252 142 397 9.72e-51 171
MS.gene004445.t1 MTR_2g437170 41.603 262 132 7 2 250 137 390 1.78e-49 168
MS.gene004445.t1 MTR_2g437150 40.520 269 139 7 2 257 137 397 3.28e-49 167
MS.gene004445.t1 MTR_2g437180 41.339 254 140 6 2 250 131 380 8.85e-49 166
MS.gene004445.t1 MTR_4g120380 38.583 254 142 4 2 248 124 370 6.38e-47 160
MS.gene004445.t1 MTR_3g450500 34.630 257 160 4 1 250 117 372 7.35e-44 152
MS.gene004445.t1 MTR_2g437940 45.641 195 102 3 63 255 167 359 1.22e-43 152
MS.gene004445.t1 MTR_2g437160 37.288 236 131 4 32 250 69 304 1.45e-43 150
MS.gene004445.t1 MTR_4g119580 36.546 249 154 3 2 248 124 370 3.83e-41 145
MS.gene004445.t1 MTR_2g437090 47.500 160 82 2 97 254 3 162 1.66e-40 138
MS.gene004445.t1 MTR_4g120380 37.662 231 130 4 2 225 124 347 4.47e-40 143
MS.gene004445.t1 MTR_4g007490 33.074 257 165 4 1 251 116 371 2.28e-37 135
MS.gene004445.t1 MTR_4g119570 34.677 248 125 4 3 248 17 229 3.84e-33 120
MS.gene004445.t1 MTR_2g019830 81.159 69 13 0 187 255 136 204 6.41e-27 103
MS.gene004445.t1 MTR_8g080990 29.719 249 168 4 1 244 122 368 1.12e-23 99.0
MS.gene004445.t1 MTR_8g081000 26.587 252 180 4 1 248 135 385 2.47e-21 92.4
MS.gene004445.t1 MTR_8g081000 26.587 252 180 4 1 248 135 385 2.81e-21 92.4
MS.gene004445.t1 MTR_8g081000 26.587 252 180 4 1 248 135 385 3.47e-21 92.0
MS.gene004445.t1 MTR_2g436380 47.692 65 33 1 97 160 3 67 2.27e-11 63.2
MS.gene004445.t1 MTR_3g085240 30.370 135 77 2 108 242 132 249 3.88e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004445.t1 AT5G07900 37.402 254 151 4 1 247 147 399 1.04e-51 174
MS.gene004445.t1 AT1G21150 29.572 257 175 3 1 251 98 354 9.80e-34 125
MS.gene004445.t1 AT1G21150 29.572 257 175 3 1 251 134 390 1.85e-33 125
MS.gene004445.t1 AT1G21150 29.572 257 175 3 1 251 198 454 5.53e-33 125
MS.gene004445.t1 AT1G62010 28.854 253 131 7 2 213 117 361 6.43e-22 94.4
MS.gene004445.t1 AT1G61970 26.736 288 166 9 2 247 131 415 5.46e-21 91.7
MS.gene004445.t1 AT1G61970 26.736 288 166 9 2 247 131 415 5.46e-21 91.7
MS.gene004445.t1 AT1G61970 26.736 288 166 9 2 247 131 415 5.46e-21 91.7
MS.gene004445.t1 AT1G61970 26.736 288 166 9 2 247 131 415 5.46e-21 91.7
MS.gene004445.t1 AT1G61970 26.736 288 166 9 2 247 131 415 5.46e-21 91.7
MS.gene004445.t1 AT1G61970 26.736 288 166 9 2 247 131 415 5.46e-21 91.7
MS.gene004445.t1 AT1G61970 26.736 288 166 9 2 247 131 415 5.46e-21 91.7
MS.gene004445.t1 AT1G61980 26.644 289 165 9 2 247 131 415 1.67e-19 87.4
MS.gene004445.t1 AT1G61980 26.644 289 165 9 2 247 131 415 1.67e-19 87.4
MS.gene004445.t1 AT1G61980 26.644 289 165 9 2 247 131 415 1.67e-19 87.4
MS.gene004445.t1 AT1G61990 26.224 286 163 8 2 247 131 408 3.13e-19 86.7
MS.gene004445.t1 AT1G61990 26.224 286 163 8 2 247 131 408 3.13e-19 86.7
MS.gene004445.t1 AT1G62085 27.795 331 152 12 2 257 134 452 3.09e-16 78.2
MS.gene004445.t1 AT1G62085 27.795 331 152 12 2 257 134 452 3.09e-16 78.2
MS.gene004445.t1 AT5G64950 26.437 261 178 6 1 248 117 376 6.30e-15 74.3
MS.gene004445.t1 AT1G62110 24.204 314 151 8 2 233 131 439 1.65e-14 73.2
MS.gene004445.t1 AT5G23930 27.597 308 137 9 2 233 134 431 5.32e-14 71.6
MS.gene004445.t1 AT1G61960 32.335 167 104 6 2 163 131 293 8.26e-14 71.2
MS.gene004445.t1 AT1G61960 30.000 120 80 1 132 247 332 451 5.97e-12 65.5
MS.gene004445.t1 AT3G46950 30.303 165 105 5 2 163 131 288 1.40e-13 70.5
MS.gene004445.t1 AT1G79220 28.125 256 145 8 3 253 161 382 2.11e-12 66.6

Find 33 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAAAGATAGCAGGATTTC+TGG 0.169200 2.4:+66943850 None:intergenic
AAAACAAACACTCAGAAATT+TGG 0.172074 2.4:+66943225 None:intergenic
ATTCAAATCCTGCTTCAAAA+TGG 0.214854 2.4:-66943698 MS.gene004445:CDS
TTTGATTAAGGATGCTAAAT+TGG 0.230774 2.4:-66943258 MS.gene004445:CDS
AACAATGAAAACAGTTGTTT+TGG 0.284531 2.4:+66943562 None:intergenic
ACCAATTTCGAAACAAAATA+AGG 0.302219 2.4:+66943657 None:intergenic
TTGGTGGAAGTAAATGAATT+AGG 0.343678 2.4:-66943593 MS.gene004445:CDS
TTCAAATCCTGCTTCAAAAT+GGG 0.347056 2.4:-66943697 MS.gene004445:CDS
TTGAAATTTCTTGAGTCTAA+AGG 0.377057 2.4:-66943281 MS.gene004445:CDS
GATTGATGTGTATAAGAAAT+GGG 0.377187 2.4:-66943495 MS.gene004445:CDS
TGAGTCTAAAGGTTTGATTA+AGG 0.378829 2.4:-66943270 MS.gene004445:CDS
AGATTGATGTGTATAAGAAA+TGG 0.401224 2.4:-66943496 MS.gene004445:CDS
ATTAAGGATGCTAAATTGGC+TGG 0.417571 2.4:-66943254 MS.gene004445:CDS
TTTGCTGAGTCTTGAGAAAA+GGG 0.428056 2.4:-66943324 MS.gene004445:CDS
GACTCAGCAAAAGAAGTATT+GGG 0.431337 2.4:+66943336 None:intergenic
AGACTCAGCAAAAGAAGTAT+TGG 0.433507 2.4:+66943335 None:intergenic
AATGCCTTCTCAAATCTTGA+AGG 0.433670 2.4:+66943614 None:intergenic
GAAGTTTGTGTATTGCTTCG+AGG 0.509974 2.4:-66943198 MS.gene004445:CDS
TTCAAGATTTGAGAAGGCAT+TGG 0.516289 2.4:-66943612 MS.gene004445:CDS
GGCATCTGTTCGTAAAATTG+AGG 0.523840 2.4:-66943417 MS.gene004445:CDS
AAGATTTGAGAAGGCATTGG+TGG 0.528058 2.4:-66943609 MS.gene004445:CDS
TCAAATCCTGCTTCAAAATG+GGG 0.541281 2.4:-66943696 MS.gene004445:CDS
GAAGTATCCATGGTGTATGT+TGG 0.562557 2.4:-66943438 MS.gene004445:CDS
CATCAATCTTCCTTTCCCAA+AGG 0.572836 2.4:+66943510 None:intergenic
TTTGATAAAATGATAACAAG+GGG 0.581767 2.4:+66943805 None:intergenic
TTCTGGTGATGATTTGAACA+AGG 0.591029 2.4:+66943867 None:intergenic
AATTTCAAGACAAAAGCTCT+AGG 0.592288 2.4:+66943296 None:intergenic
GAAGCCTTCAAGATTTGAGA+AGG 0.601085 2.4:-66943618 MS.gene004445:CDS
AAACACTCAGAAATTTGGAA+AGG 0.625518 2.4:+66943230 None:intergenic
AAAATAAGGACTTTAGACTC+AGG 0.629406 2.4:+66943671 None:intergenic
ATTGATGTGTATAAGAAATG+GGG 0.652614 2.4:-66943494 MS.gene004445:CDS
ACAGATGCCAACATACACCA+TGG 0.670800 2.4:+66943431 None:intergenic
ATGTGTATAAGAAATGGGGT+TGG 0.700509 2.4:-66943490 MS.gene004445:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATAAAAAAATCCTT+TGG - chr2.4:66943901-66943920 MS.gene004445:intron 10.0%
!! AATTAATAAAAAAATCCTTT+GGG - chr2.4:66943902-66943921 MS.gene004445:intron 10.0%
!!! TTTTGATAAAATGATAACAA+GGG + chr2.4:66943626-66943645 None:intergenic 15.0%
!! TTTGATAAAATGATAACAAG+GGG + chr2.4:66943625-66943644 None:intergenic 20.0%
!!! CTTTTGATAAAATGATAACA+AGG + chr2.4:66943627-66943646 None:intergenic 20.0%
! AAAACAAACACTCAGAAATT+TGG + chr2.4:66944205-66944224 None:intergenic 25.0%
! AACTATCTCATTCAAAATCT+TGG - chr2.4:66943318-66943337 MS.gene004445:CDS 25.0%
! ACCAATTTCGAAACAAAATA+AGG + chr2.4:66943773-66943792 None:intergenic 25.0%
! ACTATCTCATTCAAAATCTT+GGG - chr2.4:66943319-66943338 MS.gene004445:CDS 25.0%
! AGAAAAAAGATGTTTCCAAT+AGG - chr2.4:66944285-66944304 MS.gene004445:intron 25.0%
! AGATTGATGTGTATAAGAAA+TGG - chr2.4:66943931-66943950 MS.gene004445:intron 25.0%
! ATTGATGTGTATAAGAAATG+GGG - chr2.4:66943933-66943952 MS.gene004445:intron 25.0%
! CTTTCATTATTCAAATCACA+TGG - chr2.4:66943414-66943433 MS.gene004445:CDS 25.0%
! GATTGATGTGTATAAGAAAT+GGG - chr2.4:66943932-66943951 MS.gene004445:intron 25.0%
! TTGAAATTTCTTGAGTCTAA+AGG - chr2.4:66944146-66944165 MS.gene004445:intron 25.0%
! TTTGATTAAGGATGCTAAAT+TGG - chr2.4:66944169-66944188 MS.gene004445:intron 25.0%
!! AACAATGAAAACAGTTGTTT+TGG + chr2.4:66943868-66943887 None:intergenic 25.0%
!! AAGATTTTGAATGAGATAGT+TGG + chr2.4:66943319-66943338 None:intergenic 25.0%
!! AAGCTTTTATAAAAGATAGC+AGG + chr2.4:66943588-66943607 None:intergenic 25.0%
!! ATAAAAAAATCCTTTGGGAA+AGG - chr2.4:66943907-66943926 MS.gene004445:intron 25.0%
!! GATCTAAAGAGAGAATTTTT+GGG + chr2.4:66943479-66943498 None:intergenic 25.0%
!! TGCTATCTTTTATAAAAGCT+TGG - chr2.4:66943587-66943606 MS.gene004445:CDS 25.0%
!!! ACAATGAAAACAGTTGTTTT+GGG + chr2.4:66943867-66943886 None:intergenic 25.0%
!!! ATCTTTTTTCAACATCCTAT+TGG + chr2.4:66944303-66944322 None:intergenic 25.0%
!!! GAAATTTTTTGTTGATCACA+TGG - chr2.4:66944040-66944059 MS.gene004445:intron 25.0%
!!! TCCTTATTTTGTTTCGAAAT+TGG - chr2.4:66943769-66943788 MS.gene004445:CDS 25.0%
AAAATAAGGACTTTAGACTC+AGG + chr2.4:66943759-66943778 None:intergenic 30.0%
AAAATTGAGTTTGGGAAGAA+TGG + chr2.4:66943511-66943530 None:intergenic 30.0%
AAACACTCAGAAATTTGGAA+AGG + chr2.4:66944200-66944219 None:intergenic 30.0%
AAACTGAATGTGAGGTTATT+GGG + chr2.4:66943207-66943226 None:intergenic 30.0%
AATAATGAAAGAACTGAGTC+TGG + chr2.4:66943405-66943424 None:intergenic 30.0%
AATCAGAGTAACAAGATTGT+TGG + chr2.4:66943129-66943148 None:intergenic 30.0%
AATTTCAAGACAAAAGCTCT+AGG + chr2.4:66944134-66944153 None:intergenic 30.0%
ATAAAAGATAGCAGGATTTC+TGG + chr2.4:66943580-66943599 None:intergenic 30.0%
ATTCAAATCCTGCTTCAAAA+TGG - chr2.4:66943729-66943748 MS.gene004445:CDS 30.0%
GAGAGAAGAAAATTGAGTTT+GGG + chr2.4:66943519-66943538 None:intergenic 30.0%
TGAGAGAAGAAAATTGAGTT+TGG + chr2.4:66943520-66943539 None:intergenic 30.0%
TGAGTCTAAAGGTTTGATTA+AGG - chr2.4:66944157-66944176 MS.gene004445:intron 30.0%
TTCAAATCCTGCTTCAAAAT+GGG - chr2.4:66943730-66943749 MS.gene004445:CDS 30.0%
TTGGTGGAAGTAAATGAATT+AGG - chr2.4:66943834-66943853 MS.gene004445:CDS 30.0%
! ACTCAATTTTCTTCTCTCAA+AGG - chr2.4:66943521-66943540 MS.gene004445:CDS 30.0%
! ATGAAGATTGTGTTACTGTT+AGG + chr2.4:66943236-66943255 None:intergenic 30.0%
! CTCAATTTTCTTCTCTCAAA+GGG - chr2.4:66943522-66943541 MS.gene004445:CDS 30.0%
! GGATCTAAAGAGAGAATTTT+TGG + chr2.4:66943480-66943499 None:intergenic 30.0%
! TGAAGATTGTGTTACTGTTA+GGG + chr2.4:66943235-66943254 None:intergenic 30.0%
! TTGAATTTTGTTGGAGAGTT+TGG + chr2.4:66943364-66943383 None:intergenic 30.0%
! TTTTTGTTGATCACATGGAT+TGG - chr2.4:66944045-66944064 MS.gene004445:intron 30.0%
!! CAATGAAAACAGTTGTTTTG+GGG + chr2.4:66943866-66943885 None:intergenic 30.0%
!!! TTGGGAAGAATGGTTTTATT+GGG + chr2.4:66943501-66943520 None:intergenic 30.0%
!!! TTTGGGAAGAATGGTTTTAT+TGG + chr2.4:66943502-66943521 None:intergenic 30.0%
AACTGAATGTGAGGTTATTG+GGG + chr2.4:66943206-66943225 None:intergenic 35.0%
AAGATGAAGAAGAAAAGGGT+TGG + chr2.4:66943275-66943294 None:intergenic 35.0%
AATGCCTTCTCAAATCTTGA+AGG + chr2.4:66943816-66943835 None:intergenic 35.0%
ATGTGTATAAGAAATGGGGT+TGG - chr2.4:66943937-66943956 MS.gene004445:intron 35.0%
ATTAAGGATGCTAAATTGGC+TGG - chr2.4:66944173-66944192 MS.gene004445:intron 35.0%
GAAACTGAATGTGAGGTTAT+TGG + chr2.4:66943208-66943227 None:intergenic 35.0%
TCAAATCCTGCTTCAAAATG+GGG - chr2.4:66943731-66943750 MS.gene004445:CDS 35.0%
TGGGAAGATGAAGAAGAAAA+GGG + chr2.4:66943279-66943298 None:intergenic 35.0%
TTGGGAAGATGAAGAAGAAA+AGG + chr2.4:66943280-66943299 None:intergenic 35.0%
TTTGCTGAGTCTTGAGAAAA+GGG - chr2.4:66944103-66944122 MS.gene004445:intron 35.0%
! AGACTCAGCAAAAGAAGTAT+TGG + chr2.4:66944095-66944114 None:intergenic 35.0%
! TTCAAGATTTGAGAAGGCAT+TGG - chr2.4:66943815-66943834 MS.gene004445:CDS 35.0%
! TTCTGGTGATGATTTGAACA+AGG + chr2.4:66943563-66943582 None:intergenic 35.0%
! TTTTGCTGAGTCTTGAGAAA+AGG - chr2.4:66944102-66944121 MS.gene004445:intron 35.0%
!! GACTCAGCAAAAGAAGTATT+GGG + chr2.4:66944094-66944113 None:intergenic 35.0%
!! GGTTGTGAAAGTGTGATTTT+GGG + chr2.4:66943298-66943317 None:intergenic 35.0%
!! TGGTTGTGAAAGTGTGATTT+TGG + chr2.4:66943299-66943318 None:intergenic 35.0%
!! TTTGATGAGAGTTGATAGCT+GGG + chr2.4:66943451-66943470 None:intergenic 35.0%
!!! CAGCATTTTTGAAGTATCCA+TGG - chr2.4:66943979-66943998 MS.gene004445:intron 35.0%
!!! GTCGTTGAGTTGAATTTTGT+TGG + chr2.4:66943373-66943392 None:intergenic 35.0%
!!! TTTTGATGAGAGTTGATAGC+TGG + chr2.4:66943452-66943471 None:intergenic 35.0%
CATCAATCTTCCTTTCCCAA+AGG + chr2.4:66943920-66943939 None:intergenic 40.0%
GAAGCCTTCAAGATTTGAGA+AGG - chr2.4:66943809-66943828 MS.gene004445:CDS 40.0%
GAAGTTTGTGTATTGCTTCG+AGG - chr2.4:66944229-66944248 MS.gene004445:intron 40.0%
GGCATCTGTTCGTAAAATTG+AGG - chr2.4:66944010-66944029 MS.gene004445:intron 40.0%
! GAAGTATCCATGGTGTATGT+TGG - chr2.4:66943989-66944008 MS.gene004445:intron 40.0%
!! AAGATTTGAGAAGGCATTGG+TGG - chr2.4:66943818-66943837 MS.gene004445:CDS 40.0%
!!! GGGTTTTGAAACTGAATGTG+AGG + chr2.4:66943215-66943234 None:intergenic 40.0%
ACAGATGCCAACATACACCA+TGG + chr2.4:66943999-66944018 None:intergenic 45.0%
GAGAGTTTGGAAGCAGTTTC+TGG + chr2.4:66943351-66943370 None:intergenic 45.0%
! GGGTGTTTTGCAAGCACAAT+AGG + chr2.4:66944074-66944093 None:intergenic 45.0%
!! GCAGTTTTTGAGAGATGCGA+TGG + chr2.4:66943673-66943692 None:intergenic 45.0%
!!! CAGGAACCCCATTTTGAAGC+AGG + chr2.4:66943740-66943759 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 66943115 66944334 66943115 ID=MS.gene004445
chr2.4 mRNA 66943115 66944334 66943115 ID=MS.gene004445.t1;Parent=MS.gene004445
chr2.4 exon 66944332 66944334 66944332 ID=MS.gene004445.t1.exon1;Parent=MS.gene004445.t1
chr2.4 CDS 66944332 66944334 66944332 ID=cds.MS.gene004445.t1;Parent=MS.gene004445.t1
chr2.4 exon 66943115 66943906 66943115 ID=MS.gene004445.t1.exon2;Parent=MS.gene004445.t1
chr2.4 CDS 66943115 66943906 66943115 ID=cds.MS.gene004445.t1;Parent=MS.gene004445.t1
Gene Sequence

>MS.gene004445

ATGGTATGAATCCAACAATCTTGTTACTCTGATTTTGTTTATATTTTTCAATCATCATGTTTTGTCACTATCGCAAAGCATTGCTCTTCCCCAATAACCTCACATTCAGTTTCAAAACCCTAACAGTAACACAATCTTCATTTCTTCTTCATCTTCTCCAACCCTTTTCTTCTTCATCTTCCCAAAATCACACTTTCACAACCAACTATCTCATTCAAAATCTTGGGTTATCACCAGAAACTGCTTCCAAACTCTCCAACAAAATTCAACTCAACGACTCACAAAAACCAGACTCAGTTCTTTCATTATTCAAATCACATGGCTTTTCAAACTCCCAGCTATCAACTCTCATCAAAACTCACCCAAAAATTCTCTCTTTAGATCCCAATAAAACCATTCTTCCCAAACTCAATTTTCTTCTCTCAAAGGGTGCTTCAAATTCAGACCTTGTTCAAATCATCACCAGAAATCCTGCTATCTTTTATAAAAGCTTGGAAAATACAATAACCCCTTGTTATCATTTTATCAAAAGATTCTTACTTTCTGATCAATCTACCATCGCATCTCTCAAAAACTGCACTTGTTTGCTTTATTCTAAAACTACGTCTCAGAATATTCAAATCCTGCTTCAAAATGGGGTTCCTGAGTCTAAAGTCCTTATTTTGTTTCGAAATTGGTATTCTATATTCGCTGAGAAGCCTTCAAGATTTGAGAAGGCATTGGTGGAAGTAAATGAATTAGGATTTAACCCCAAAACAACTGTTTTCATTGTTGCACTGCGTGCTAAAATTAATAAAAAAATCCTTTGGGAAAGGAAGATTGATGTGTATAAGAAATGGGGTTGGTCTGAAGAAAGTTTTGTCTCAGCATTTTTGAAGTATCCATGGTGTATGTTGGCATCTGTTCGTAAAATTGAGGCAGCAATGAAATTTTTTGTTGATCACATGGATTGGAAGCCTATTGTGCTTGCAAAACACCCAATACTTCTTTTGCTGAGTCTTGAGAAAAGGGTTATTCCTAGAGCTTTTGTCTTGAAATTTCTTGAGTCTAAAGGTTTGATTAAGGATGCTAAATTGGCTGGACCTTTCCAAATTTCTGAGTGTTTGTTTTTGAAGAAGTTTGTGTATTGCTTCGAGGAAGAAGCTTCTCAACTGTTGAAGTTGTACGAAGAGAAAAAAGATGTTTCCAATAGGATGTTGAAAAAAGATTTTAAGCCGTGA
Protein sequence

>MS.gene004445.t1

MGASNSDLVQIITRNPAIFYKSLENTITPCYHFIKRFLLSDQSTIASLKNCTCLLYSKTTSQNIQILLQNGVPESKVLILFRNWYSIFAEKPSRFEKALVEVNELGFNPKTTVFIVALRAKINKKILWERKIDVYKKWGWSEESFVSAFLKYPWCMLASVRKIEAAMKFFVDHMDWKPIVLAKHPILLLLSLEKRVIPRAFVLKFLESKGLIKDAKLAGPFQISECLFLKKFVYCFEEEASQLLKLYEEKKDVSNRMLKKDFKP