AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00448

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00448.t1 MTR_1g019130 98.305 236 4 0 1 236 1 236 1.34e-175 482
MS.gene00448.t1 MTR_1g019130 97.861 187 4 0 1 187 1 187 1.55e-135 380
MS.gene00448.t1 MTR_8g107210 45.255 137 69 1 22 152 22 158 2.87e-29 112
MS.gene00448.t1 MTR_7g060630 71.642 67 19 0 86 152 4 70 7.40e-29 108
MS.gene00448.t1 MTR_4g084550 68.750 64 20 0 89 152 63 126 1.77e-26 101
MS.gene00448.t1 MTR_4g063735 36.932 176 81 4 90 235 25 200 2.33e-26 103
MS.gene00448.t1 MTR_5g081990 65.152 66 23 0 90 155 33 98 1.14e-25 99.4
MS.gene00448.t1 MTR_5g021930 64.062 64 22 1 89 151 29 92 1.54e-22 94.0
MS.gene00448.t1 MTR_3g088485 40.146 137 60 2 22 152 22 142 8.33e-22 88.6
MS.gene00448.t1 MTR_8g095580 76.923 52 12 0 101 152 93 144 3.55e-21 87.0
MS.gene00448.t1 MTR_2g015000 35.577 104 55 1 49 152 26 117 5.00e-14 71.2
MS.gene00448.t1 MTR_7g025010 46.774 62 33 0 90 151 47 108 2.79e-13 68.9
MS.gene00448.t1 MTR_4g088070 37.778 90 44 1 61 150 12 89 2.25e-12 66.2
MS.gene00448.t1 MTR_3g115620 39.474 76 46 0 90 165 82 157 4.15e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00448.t1 AT1G46480 52.896 259 91 9 1 236 1 251 1.58e-72 221
MS.gene00448.t1 AT1G46480 59.067 193 60 7 1 182 1 185 2.95e-63 196
MS.gene00448.t1 AT3G18010 42.857 147 74 2 6 152 2 138 5.96e-30 114
MS.gene00448.t1 AT3G11260 56.818 88 33 1 87 169 16 103 1.21e-27 104
MS.gene00448.t1 AT5G59340 61.333 75 29 0 78 152 2 76 5.60e-27 104
MS.gene00448.t1 AT2G28610 68.182 66 21 0 87 152 5 70 9.58e-27 103
MS.gene00448.t1 AT5G05770 63.077 65 24 0 90 154 29 93 1.68e-24 94.4
MS.gene00448.t1 AT2G17950 51.765 85 40 1 89 172 37 121 1.02e-23 96.7
MS.gene00448.t1 AT2G01500 66.667 63 19 1 84 144 53 115 2.02e-21 90.1
MS.gene00448.t1 AT2G33880 38.614 101 52 1 49 149 24 114 4.34e-14 71.2
MS.gene00448.t1 AT2G33880 38.614 101 52 1 49 149 24 114 4.61e-14 70.9
MS.gene00448.t1 AT1G20700 47.541 61 32 0 90 150 33 93 2.65e-13 65.9
MS.gene00448.t1 AT5G45980 46.774 62 33 0 90 151 55 116 4.88e-13 67.8
MS.gene00448.t1 AT1G20710 38.462 91 44 1 97 187 86 164 6.39e-13 65.9
MS.gene00448.t1 AT1G20700 47.541 61 32 0 90 150 95 155 8.99e-13 65.5
MS.gene00448.t1 AT1G20700 47.541 61 32 0 90 150 95 155 1.07e-12 65.5
MS.gene00448.t1 AT4G35550 47.541 61 32 0 90 150 99 159 1.13e-11 63.2

Find 60 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTCACACGTGGATTT+TGG 0.121803 1.3:-4327634 MS.gene00448:CDS
TCTCGCGTGCTTTGTGGTTT+TGG 0.203041 1.3:+4326801 None:intergenic
AAACTCCTTCATCTGCCTTC+TGG 0.237007 1.3:+4326266 None:intergenic
AACTCCTTCATCTGCCTTCT+GGG 0.285945 1.3:+4326267 None:intergenic
CAACTTTGGACACCCCAAAA+AGG 0.296385 1.3:-4326682 MS.gene00448:CDS
ACTAATTCTAGGAGCACTTT+CGG 0.300053 1.3:+4327478 None:intergenic
CTAATTCTAGGAGCACTTTC+GGG 0.314910 1.3:+4327479 None:intergenic
ACAAGAACAAATAGGAATAT+TGG 0.328145 1.3:-4326936 MS.gene00448:CDS
GAGGAAGAAAGACTAATTCT+AGG 0.342909 1.3:+4327467 None:intergenic
TGTTCTATTTGCTGTGCATT+TGG 0.355896 1.3:+4326884 None:intergenic
GGATTCCACCTTGTCCCTCC+TGG 0.366142 1.3:+4326962 None:intergenic
ATCTGCCTTCTGGGTGTAAT+GGG 0.376276 1.3:+4326276 None:intergenic
CTTTAGTACTATTACAACTT+TGG 0.384910 1.3:-4326696 MS.gene00448:CDS
CATCTGCCTTCTGGGTGTAA+TGG 0.398760 1.3:+4326275 None:intergenic
ATTCCTATTTGTTCTTGTGT+TGG 0.415008 1.3:+4326941 None:intergenic
AATCCAACACAAGAACAAAT+AGG 0.426122 1.3:-4326944 MS.gene00448:CDS
TTCTCTTCCAAGTACTCAAA+TGG 0.427494 1.3:+4326347 None:intergenic
TTTGTCTCTCGCGTGCTTTG+TGG 0.448508 1.3:+4326795 None:intergenic
TAGGTTGAAACGCATATTCC+AGG 0.450427 1.3:-4326980 MS.gene00448:CDS
TCCACCTTCACCGCAAAGCC+AGG 0.461999 1.3:-4327426 MS.gene00448:intron
GAGTTGGCCATTTGAGTACT+TGG 0.464745 1.3:-4326354 MS.gene00448:CDS
GGTGTTAGAAGTAACATTAT+CGG 0.465029 1.3:+4327538 None:intergenic
CACAGACACAAGTGTGGTGG+TGG 0.469900 1.3:+4326727 None:intergenic
CAAAGTTGTAATAGTACTAA+AGG 0.472738 1.3:+4326697 None:intergenic
AGCAAGTACGGTAAAATTGA+AGG 0.477134 1.3:-4326845 MS.gene00448:CDS
CAACTCCTACTCTTCTTCAA+TGG 0.501838 1.3:+4326371 None:intergenic
AGTGTGGTGGTGGGAGTTCG+AGG 0.518160 1.3:+4326737 None:intergenic
CATGAACCTTCCCTCACACT+TGG 0.520457 1.3:-4327608 MS.gene00448:CDS
TGAACCTGGCTTTGCGGTGA+AGG 0.521265 1.3:+4327422 None:intergenic
GTTGTAATAGTACTAAAGGT+GGG 0.528602 1.3:+4326701 None:intergenic
TGAGATCAAAAGAAAGAGAA+GGG 0.531578 1.3:+4327516 None:intergenic
AGTATATGAACCTGGCTTTG+CGG 0.544738 1.3:+4327416 None:intergenic
ATAGTCCATTGAAGAAGAGT+AGG 0.545832 1.3:-4326376 MS.gene00448:CDS
AAGCAGAAACGTAACAGTCT+TGG 0.547628 1.3:-4326773 MS.gene00448:CDS
ACAGTTCAACTTAGCAAGTA+CGG 0.553808 1.3:-4326857 MS.gene00448:CDS
ATCTTTCTTGTTATCATCAG+AGG 0.556963 1.3:+4327448 None:intergenic
CGCATATTCCAGGAGGGACA+AGG 0.565252 1.3:-4326970 MS.gene00448:CDS
TACACCCAGAAGGCAGATGA+AGG 0.566613 1.3:-4326271 None:intergenic
GTGGGAGTTCGAGGACTATG+AGG 0.567688 1.3:+4326746 None:intergenic
TTGGTCAATGTGCAAAGTAG+AGG 0.568050 1.3:-4326321 MS.gene00448:CDS
TTGAGATCAAAAGAAAGAGA+AGG 0.574637 1.3:+4327515 None:intergenic
AGTTGTAATAGTACTAAAGG+TGG 0.581733 1.3:+4326700 None:intergenic
AACGCTTGCAACCAAGTGTG+AGG 0.582297 1.3:+4327597 None:intergenic
GCAAGTACGGTAAAATTGAA+GGG 0.588273 1.3:-4326844 MS.gene00448:CDS
CATTGAAGAAGAGTAGGAGT+TGG 0.590517 1.3:-4326370 MS.gene00448:CDS
ACCTGGCTTTGCGGTGAAGG+TGG 0.590968 1.3:+4327425 None:intergenic
TGTAATAGTACTAAAGGTGG+GGG 0.603492 1.3:+4326703 None:intergenic
TTGTAATAGTACTAAAGGTG+GGG 0.606911 1.3:+4326702 None:intergenic
TTGGAGATGTTATACAGAGG+TGG 0.611718 1.3:-4326917 MS.gene00448:CDS
GAGTTCGAGGACTATGAGGA+AGG 0.633348 1.3:+4326750 None:intergenic
ACGCTTGCAACCAAGTGTGA+GGG 0.656705 1.3:+4327598 None:intergenic
TTGAAACGCATATTCCAGGA+GGG 0.657126 1.3:-4326976 MS.gene00448:CDS
ACAGACACAAGTGTGGTGGT+GGG 0.659851 1.3:+4326728 None:intergenic
GGACACAGACACAAGTGTGG+TGG 0.666759 1.3:+4326724 None:intergenic
GAGATCAAAAGAAAGAGAAG+GGG 0.671823 1.3:+4327517 None:intergenic
TTGCAACCAAGTGTGAGGGA+AGG 0.674259 1.3:+4327602 None:intergenic
GTTGAAACGCATATTCCAGG+AGG 0.708848 1.3:-4326977 MS.gene00448:CDS
ATATTGGAGATGTTATACAG+AGG 0.712720 1.3:-4326920 MS.gene00448:CDS
AAAGTGCATCAATTCACACG+TGG 0.742454 1.3:-4327641 MS.gene00448:CDS
ATATTCCAGGAGGGACAAGG+TGG 0.756182 1.3:-4326967 MS.gene00448:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATACTAATAAAGTATTT+TGG - chr1.3:4326704-4326723 MS.gene00448:CDS 10.0%
!! ACAGACTTAATTAAAAAAAA+TGG + chr1.3:4326549-4326568 None:intergenic 15.0%
!! AATACTATAATCTTGTTACT+AGG - chr1.3:4327446-4327465 MS.gene00448:CDS 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1.3:4327424-4327443 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1.3:4326661-4326680 MS.gene00448:intron 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1.3:4326919-4326938 MS.gene00448:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1.3:4326662-4326681 MS.gene00448:intron 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1.3:4326361-4326380 None:intergenic 20.0%
!!! TCGAATATGTTACATTTTTT+TGG - chr1.3:4327487-4327506 MS.gene00448:CDS 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1.3:4327411-4327430 None:intergenic 20.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1.3:4326982-4327001 MS.gene00448:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1.3:4327224-4327243 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1.3:4327222-4327241 MS.gene00448:intron 25.0%
! TACATCTGATAACTGAAATA+CGG + chr1.3:4326749-4326768 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1.3:4327282-4327301 MS.gene00448:intron 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1.3:4327252-4327271 None:intergenic 25.0%
!! CGACAATATAAAGGATTTTA+AGG + chr1.3:4327330-4327349 None:intergenic 25.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1.3:4326709-4326728 MS.gene00448:CDS 25.0%
!!! TATGTTACATTTTTTTGGAC+AGG - chr1.3:4327492-4327511 MS.gene00448:CDS 25.0%
!!! TCGTTTAAAAGCTTTTGATT+TGG - chr1.3:4326869-4326888 MS.gene00448:CDS 25.0%
!!! TTTTTGATTAAAAGGAACGT+AGG - chr1.3:4327384-4327403 MS.gene00448:intron 25.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1.3:4327092-4327111 MS.gene00448:intron 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1.3:4326974-4326993 MS.gene00448:CDS 30.0%
AATTATACTACTCTACTGCA+TGG + chr1.3:4326576-4326595 None:intergenic 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1.3:4327578-4327597 MS.gene00448:CDS 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1.3:4327221-4327240 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1.3:4326998-4327017 MS.gene00448:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1.3:4326980-4326999 None:intergenic 30.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1.3:4326383-4326402 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1.3:4327220-4327239 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1.3:4326405-4326424 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1.3:4326406-4326425 None:intergenic 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1.3:4327219-4327238 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1.3:4327251-4327270 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1.3:4326473-4326492 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1.3:4327253-4327272 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1.3:4326710-4326729 MS.gene00448:CDS 30.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1.3:4327061-4327080 MS.gene00448:intron 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1.3:4327073-4327092 MS.gene00448:intron 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1.3:4327542-4327561 MS.gene00448:CDS 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1.3:4326404-4326423 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1.3:4326454-4326473 None:intergenic 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1.3:4327074-4327093 MS.gene00448:intron 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1.3:4326513-4326532 None:intergenic 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1.3:4327218-4327237 None:intergenic 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1.3:4327574-4327593 None:intergenic 35.0%
! ATCAATTCACACGTGGATTT+TGG - chr1.3:4326284-4326303 MS.gene00448:CDS 35.0%
! TAGTTGCGTCGACAATATAA+AGG + chr1.3:4327339-4327358 None:intergenic 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1.3:4326285-4326304 MS.gene00448:CDS 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1.3:4326443-4326462 None:intergenic 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1.3:4326352-4326371 None:intergenic 35.0%
!! TGTTCTATTTGCTGTGCATT+TGG + chr1.3:4327037-4327056 None:intergenic 35.0%
AAAGTGCATCAATTCACACG+TGG - chr1.3:4326277-4326296 MS.gene00448:CDS 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1.3:4327237-4327256 MS.gene00448:intron 40.0%
AAGCAGAAACGTAACAGTCT+TGG - chr1.3:4327145-4327164 MS.gene00448:intron 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1.3:4326820-4326839 None:intergenic 40.0%
ATACTACTCTACTGCATGGA+TGG + chr1.3:4326572-4326591 None:intergenic 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1.3:4327550-4327569 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1.3:4327548-4327567 MS.gene00448:CDS 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1.3:4326811-4326830 None:intergenic 40.0%
TACGGTTCAACAATTTCTCC+CGG + chr1.3:4326731-4326750 None:intergenic 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1.3:4326942-4326961 MS.gene00448:CDS 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1.3:4327001-4327020 MS.gene00448:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1.3:4327597-4327616 MS.gene00448:CDS 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1.3:4326938-4326957 MS.gene00448:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1.3:4327504-4327523 MS.gene00448:CDS 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1.3:4326505-4326524 None:intergenic 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1.3:4326442-4326461 None:intergenic 40.0%
AGGTCTCTGCAACGACATTT+CGG + chr1.3:4327310-4327329 None:intergenic 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1.3:4327645-4327664 None:intergenic 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1.3:4327236-4327255 MS.gene00448:intron 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1.3:4327564-4327583 MS.gene00448:CDS 45.0%
GTTGAAACGCATATTCCAGG+AGG - chr1.3:4326941-4326960 MS.gene00448:CDS 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1.3:4327345-4327364 MS.gene00448:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1.3:4327637-4327656 MS.gene00448:CDS 45.0%
!! ATCAAAAAAAAAAAAAATTA+CGG + chr1.3:4327372-4327391 None:intergenic 5.0%
!!! TTTTTTTTTTTTTGATTAAA+AGG - chr1.3:4327376-4327395 MS.gene00448:intron 5.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1.3:4326324-4326343 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1.3:4327193-4327212 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1.3:4326323-4326342 None:intergenic 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1.3:4326951-4326970 MS.gene00448:CDS 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1.3:4327646-4327665 None:intergenic 50.0%
CATGAACCTTCCCTCACACT+TGG - chr1.3:4326310-4326329 MS.gene00448:CDS 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1.3:4327171-4327190 None:intergenic 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1.3:4326319-4326338 None:intergenic 50.0%
TTTGTCTCTCGCGTGCTTTG+TGG + chr1.3:4327126-4327145 None:intergenic 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1.3:4327120-4327139 None:intergenic 50.0%
CACAGACACAAGTGTGGTGG+TGG + chr1.3:4327194-4327213 None:intergenic 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1.3:4326948-4326967 MS.gene00448:CDS 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1.3:4327197-4327216 None:intergenic 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1.3:4327175-4327194 None:intergenic 55.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1.3:4326499-4326518 None:intergenic 55.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1.3:4326959-4326978 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1.3:4327200-4327219 None:intergenic 60.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1.3:4326492-4326511 MS.gene00448:intron 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1.3:4326496-4326515 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1.3:4327184-4327203 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 4326274 4327666 4326274 ID=MS.gene00448
chr1.3 mRNA 4326274 4327666 4326274 ID=MS.gene00448.t1;Parent=MS.gene00448
chr1.3 exon 4327427 4327666 4327427 ID=MS.gene00448.t1.exon1;Parent=MS.gene00448.t1
chr1.3 CDS 4327427 4327666 4327427 ID=cds.MS.gene00448.t1;Parent=MS.gene00448.t1
chr1.3 exon 4326682 4326999 4326682 ID=MS.gene00448.t1.exon2;Parent=MS.gene00448.t1
chr1.3 CDS 4326682 4326999 4326682 ID=cds.MS.gene00448.t1;Parent=MS.gene00448.t1
chr1.3 exon 4326274 4326426 4326274 ID=MS.gene00448.t1.exon3;Parent=MS.gene00448.t1
chr1.3 CDS 4326274 4326426 4326274 ID=cds.MS.gene00448.t1;Parent=MS.gene00448.t1
Gene Sequence

>MS.gene00448

ATGAAAGTGCATCAATTCACACGTGGATTTTGGGAGCATGAACCTTCCCTCACACTTGGTTGCAAGCGTTTACGTCCTCTTGCACCTAAAATTTCTTCTAATAACACCGATAATGTTACTTCTAACACCCCTTCTCTTTCTTTTGATCTCAAGAGCTTCATTAGACCCGAAAGTGCTCCTAGAATTAGTCTTTCTTCCTCTGATGATAACAAGAAAGATCCACCTTCACCGCAAAGCCAGGTTCATATACTAAGCTCTACTTAGTTTCATTTCCATTTTTTTTAATTAAGTCTGTCCATCCATGCAGTAGAGTAGTATAATTAACTCACGTGTGCGTATAATGCAATAAAAAATTAAGTATAATCAAGAACAACATAAATAATTCACATTAACTTCATGTAACATATAGGGTAATAACATATTTTTTACTATAATACTAATAAAGTATTTTGGTCCGGGAGAAATTGTTGAACCGTATTTCAGTTATCAGATGTATCTATATTTAGTGTATGATAATTCTTCAAACATAAACTTCCTCTTTTTCCATATGAGACTCGAGAGTTACTTGAAAAAAATATTAAACGACAAATATTTGTCGTTTAAAAGCTTTTGATTTGGCAATTGTATGCTAATTATTTATCATGATCTATTATTTGTGTATGTATAGGTTGAAACGCATATTCCAGGAGGGACAAGGTGGAATCCAACACAAGAACAAATAGGAATATTGGAGATGTTATACAGAGGTGGAATGAGAACTCCAAATGCACAGCAAATAGAACAAATCACAGTTCAACTTAGCAAGTACGGTAAAATTGAAGGGAAAAATGTGTTCTATTGGTTCCAAAACCACAAAGCACGCGAGAGACAAAAGCAGAAACGTAACAGTCTTGGCCTTCCTCATAGTCCTCGAACTCCCACCACCACACTTGTGTCTGTGTCCCCCACCTTTAGTACTATTACAACTTTGGACACCCCAAAAAGGGTATGTTCATATTCAAATAATATTCAACTTAAGCTATATATTGTGGTTCCGAAATGTCGTTGCAGAGACCTTAAAATCCTTTATATTGTCGACGCAACTATAGTCTTGGACCGTAATTTTTTTTTTTTTTGATTAAAAGGAACGTAGGACCGTAATTTAAAACCATGAACAATAGTATACTAATTAAAATACTATAATCTTGTTACTAGGTTAAATTTGTATATTTAATCGAATATGTTACATTTTTTTGGACAGGTGCAAGTAATGGAAAGAGATCAAGAAGATAGTCCATTGAAGAAGAGTAGGAGTTGGCCATTTGAGTACTTGGAAGAGAAAAATTGGTCAATGTGCAAAGTAGAGGAACATAAAACTCTAGAGCTTTTCCCATTACACCCAGAAGGCAGATGA
Protein sequence

>MS.gene00448.t1

MKVHQFTRGFWEHEPSLTLGCKRLRPLAPKISSNNTDNVTSNTPSLSFDLKSFIRPESAPRISLSSSDDNKKDPPSPQSQVETHIPGGTRWNPTQEQIGILEMLYRGGMRTPNAQQIEQITVQLSKYGKIEGKNVFYWFQNHKARERQKQKRNSLGLPHSPRTPTTTLVSVSPTFSTITTLDTPKRVQVMERDQEDSPLKKSRSWPFEYLEEKNWSMCKVEEHKTLELFPLHPEGR