AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004542

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004542.t1 MTR_2g436440 94.366 284 16 0 1 284 101 384 0.0 551
MS.gene004542.t1 MTR_2g437080 69.014 284 88 0 1 284 95 378 2.49e-143 408
MS.gene004542.t1 MTR_2g437060 69.258 283 87 0 2 284 111 393 1.39e-139 399
MS.gene004542.t1 MTR_2g437040 66.901 284 94 0 1 284 95 378 1.66e-137 393
MS.gene004542.t1 MTR_2g437030 67.606 284 92 0 1 284 181 464 2.67e-134 388
MS.gene004542.t1 MTR_2g437020 67.018 285 91 2 2 284 95 378 4.73e-133 382
MS.gene004542.t1 MTR_2g436400 67.143 280 92 0 1 280 102 381 3.09e-132 380
MS.gene004542.t1 MTR_2g437120 55.052 287 125 2 1 284 106 391 4.22e-105 312
MS.gene004542.t1 MTR_2g437170 53.979 289 128 2 1 284 110 398 1.54e-103 308
MS.gene004542.t1 MTR_2g438010 52.613 287 133 1 1 284 125 411 5.21e-103 307
MS.gene004542.t1 MTR_2g437130 52.465 284 135 0 1 284 96 379 1.16e-100 300
MS.gene004542.t1 MTR_2g437960 50.871 287 138 1 1 284 108 394 1.31e-99 298
MS.gene004542.t1 MTR_2g437160 52.797 286 133 1 1 284 118 403 1.58e-99 298
MS.gene004542.t1 MTR_2g437960 50.871 287 138 1 1 284 108 394 2.40e-99 298
MS.gene004542.t1 MTR_2g437990 51.748 286 136 1 1 284 105 390 9.39e-99 296
MS.gene004542.t1 MTR_2g438020 47.706 327 123 3 1 284 124 445 2.51e-98 297
MS.gene004542.t1 MTR_4g119550 48.772 285 145 1 1 284 104 388 4.37e-98 293
MS.gene004542.t1 MTR_2g437200 48.408 314 124 2 1 276 85 398 1.98e-97 292
MS.gene004542.t1 MTR_2g437150 51.748 286 133 2 1 281 110 395 2.58e-97 292
MS.gene004542.t1 MTR_2g076320 50.174 287 140 1 1 284 105 391 2.83e-96 289
MS.gene004542.t1 MTR_2g437100 53.025 281 127 2 1 276 115 395 3.24e-96 289
MS.gene004542.t1 MTR_2g437240 46.273 322 135 2 1 284 85 406 8.53e-95 286
MS.gene004542.t1 MTR_2g437180 49.129 287 141 2 1 284 104 388 4.43e-94 283
MS.gene004542.t1 MTR_2g437260 47.569 288 143 3 1 283 89 373 1.08e-89 272
MS.gene004542.t1 MTR_2g436460 48.929 280 142 1 1 279 100 379 3.12e-89 270
MS.gene004542.t1 MTR_2g437940 49.129 287 115 2 1 284 104 362 2.42e-86 263
MS.gene004542.t1 MTR_2g437090 68.902 164 51 0 121 284 3 166 8.71e-78 234
MS.gene004542.t1 MTR_2g019840 43.662 284 154 4 1 281 96 376 9.41e-75 233
MS.gene004542.t1 MTR_4g120380 44.161 274 153 0 1 274 97 370 1.24e-74 233
MS.gene004542.t1 MTR_4g119580 43.431 274 155 0 1 274 97 370 2.28e-74 232
MS.gene004542.t1 MTR_2g019810 44.649 271 146 3 8 276 91 359 3.33e-72 226
MS.gene004542.t1 MTR_2g437160 51.598 219 98 3 69 284 99 312 7.28e-69 216
MS.gene004542.t1 MTR_4g120380 42.857 266 151 1 1 265 97 362 9.98e-69 219
MS.gene004542.t1 MTR_4g119570 36.948 249 124 1 26 274 14 229 6.19e-50 165
MS.gene004542.t1 MTR_3g450500 30.961 281 184 3 4 276 94 372 1.07e-42 150
MS.gene004542.t1 MTR_4g007490 29.537 281 189 5 1 274 90 368 8.35e-34 126
MS.gene004542.t1 MTR_2g436370 58.947 95 39 0 1 95 101 195 4.99e-27 104
MS.gene004542.t1 MTR_8g080990 27.444 266 190 2 8 270 103 368 1.37e-24 102
MS.gene004542.t1 MTR_2g436380 45.133 113 55 2 121 233 3 108 2.59e-22 94.0
MS.gene004542.t1 MTR_8g081000 23.194 263 199 2 15 274 123 385 3.36e-18 84.0
MS.gene004542.t1 MTR_8g081000 23.194 263 199 2 15 274 123 385 3.38e-18 84.0
MS.gene004542.t1 MTR_8g081000 23.194 263 199 2 15 274 123 385 3.87e-18 84.0
MS.gene004542.t1 MTR_3g085240 42.697 89 34 1 178 266 176 247 4.54e-15 73.6
MS.gene004542.t1 MTR_4g119615 58.491 53 22 0 215 267 23 75 2.69e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004542.t1 AT5G07900 34.409 279 177 3 1 273 121 399 7.67e-51 172
MS.gene004542.t1 AT1G21150 29.197 274 188 3 6 273 77 350 7.06e-34 126
MS.gene004542.t1 AT1G21150 29.197 274 188 3 6 273 113 386 1.67e-33 126
MS.gene004542.t1 AT1G21150 29.197 274 188 3 6 273 177 450 4.46e-33 126
MS.gene004542.t1 AT5G64950 28.000 275 179 4 6 265 96 366 2.26e-23 98.6
MS.gene004542.t1 AT1G61970 27.243 301 172 7 1 259 104 399 2.85e-22 95.9
MS.gene004542.t1 AT1G61970 27.243 301 172 7 1 259 104 399 2.85e-22 95.9
MS.gene004542.t1 AT1G61970 27.243 301 172 7 1 259 104 399 2.85e-22 95.9
MS.gene004542.t1 AT1G61970 27.243 301 172 7 1 259 104 399 2.85e-22 95.9
MS.gene004542.t1 AT1G61970 27.243 301 172 7 1 259 104 399 2.85e-22 95.9
MS.gene004542.t1 AT1G61970 27.243 301 172 7 1 259 104 399 2.85e-22 95.9
MS.gene004542.t1 AT1G61970 27.243 301 172 7 1 259 104 399 2.85e-22 95.9
MS.gene004542.t1 AT1G61990 25.321 312 187 5 1 273 104 408 1.35e-20 91.3
MS.gene004542.t1 AT1G61990 25.321 312 187 5 1 273 104 408 1.35e-20 91.3
MS.gene004542.t1 AT1G61980 26.923 312 179 8 6 273 109 415 1.36e-20 91.3
MS.gene004542.t1 AT1G61980 26.923 312 179 8 6 273 109 415 1.36e-20 91.3
MS.gene004542.t1 AT1G61980 26.923 312 179 8 6 273 109 415 1.36e-20 91.3
MS.gene004542.t1 AT3G46950 24.925 333 173 9 2 261 104 432 3.37e-20 90.1
MS.gene004542.t1 AT1G62010 25.632 277 167 5 20 259 109 383 5.80e-19 86.7
MS.gene004542.t1 AT1G62120 26.174 298 171 7 6 259 113 405 2.76e-16 79.0
MS.gene004542.t1 AT1G62085 23.353 334 180 9 1 259 107 439 8.33e-16 77.4
MS.gene004542.t1 AT1G62085 23.353 334 180 9 1 259 107 439 8.33e-16 77.4
MS.gene004542.t1 AT1G61960 22.899 345 187 8 6 273 109 451 1.74e-14 73.6
MS.gene004542.t1 AT1G74120 26.174 149 106 2 117 264 251 396 1.95e-11 64.3

Find 64 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTTAGTGATGAACTTT+TGG 0.234643 2.1:+56945851 MS.gene004542:CDS
ATTCAAAATTCACATCTCTT+TGG 0.274713 2.1:+56945511 MS.gene004542:CDS
AGACCAACCCCATTTCTTAA+AGG 0.307244 2.1:-56945764 None:intergenic
AAACTTTGGCAACACCCTTT+TGG 0.315392 2.1:-56945329 None:intergenic
ACTAATAACCCTTGGTACTT+TGG 0.349074 2.1:-56945908 None:intergenic
CTTGGTTCTTGCTGGAAGCC+TGG 0.349228 2.1:+56945414 MS.gene004542:CDS
CAACTATAAACAAAACCTTA+TGG 0.357342 2.1:+56945725 MS.gene004542:CDS
AAGAGCCCCTTGGTTCTTGC+TGG 0.357827 2.1:+56945406 MS.gene004542:CDS
AGTCGATACCTTTAAGAAAT+GGG 0.363719 2.1:+56945756 MS.gene004542:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.1:+56945870 MS.gene004542:CDS
AAGTCGATACCTTTAAGAAA+TGG 0.369211 2.1:+56945755 MS.gene004542:CDS
GGGACTGGAGTCAGGTATTC+CGG 0.370799 2.1:+56945611 MS.gene004542:CDS
GAGGTTGGTGGAGGAATTAA+AGG 0.376599 2.1:+56945648 MS.gene004542:CDS
TTTAGTCCCAACCTACGAAT+TGG 0.397525 2.1:+56945444 MS.gene004542:CDS
GTCAGAAGTAGAAGCACCTT+TGG 0.415567 2.1:-56945365 None:intergenic
GTGCAATATCTTCTAACAAA+AGG 0.418204 2.1:+56945970 MS.gene004542:CDS
TGACATGCCAATTTAGTCCT+TGG 0.423830 2.1:-56946133 None:intergenic
CGCTTATCAGTCATTACAAA+TGG 0.438951 2.1:-56946024 None:intergenic
ATCGACTTTCTCTTTCCATA+AGG 0.444594 2.1:-56945740 None:intergenic
TGGCAAGATTGCTTCGGGAC+TGG 0.453668 2.1:+56945596 MS.gene004542:CDS
TGGAGGAATTAAAGGATTTG+AGG 0.454242 2.1:+56945656 MS.gene004542:CDS
AACGTATTATGTATGTGTTA+AGG 0.454424 2.1:-56946050 None:intergenic
TATAAACCAATTCGTAGGTT+GGG 0.458485 2.1:-56945450 None:intergenic
ATTGCTTCGGGACTGGAGTC+AGG 0.459523 2.1:+56945603 MS.gene004542:CDS
CAAACATGGCAAGATTGCTT+CGG 0.473757 2.1:+56945590 MS.gene004542:CDS
ATCTTCTAACAAAAGGTTTG+CGG 0.477453 2.1:+56945977 MS.gene004542:CDS
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2.1:+56945869 MS.gene004542:CDS
TTTCCTGCGACCCCTCCAAA+AGG 0.489524 2.1:+56945314 MS.gene004542:CDS
TATTGCTGAAGCATTTAGAA+AGG 0.489696 2.1:+56945795 MS.gene004542:CDS
AAACATGGCAAGATTGCTTC+GGG 0.506298 2.1:+56945591 MS.gene004542:CDS
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2.1:+56945879 MS.gene004542:CDS
CTATAAACCAATTCGTAGGT+TGG 0.510197 2.1:-56945451 None:intergenic
ACCTCGGTATGTCACCTGTC+CGG 0.513896 2.1:-56945630 None:intergenic
AACCCTTGGTACTTTGGCAA+TGG 0.532796 2.1:-56945902 None:intergenic
CCTTGTCAGTAAGAGCCCCT+TGG 0.547312 2.1:+56945396 MS.gene004542:CDS
TTTAATTCCTCCACCAACCT+CGG 0.560343 2.1:-56945646 None:intergenic
TTCCTGCGACCCCTCCAAAA+GGG 0.564822 2.1:+56945315 MS.gene004542:CDS
TGGAGTCAGGTATTCCGGAC+AGG 0.568713 2.1:+56945616 MS.gene004542:CDS
TCGAGAAAACTATCATACCT+AGG 0.570104 2.1:+56945938 MS.gene004542:CDS
GTCGATACCTTTAAGAAATG+GGG 0.575626 2.1:+56945757 MS.gene004542:CDS
AGACTACAACTAATAACCCT+TGG 0.576711 2.1:-56945916 None:intergenic
AGGCTTCCAGCAAGAACCAA+GGG 0.579663 2.1:-56945412 None:intergenic
TGACATACCGAGGTTGGTGG+AGG 0.582066 2.1:+56945639 MS.gene004542:CDS
CGGAGTGACCGACTCAAACA+TGG 0.586400 2.1:+56945576 MS.gene004542:CDS
CAATCTTGCCATGTTTGAGT+CGG 0.596864 2.1:-56945584 None:intergenic
AGGTGACATACCGAGGTTGG+TGG 0.600565 2.1:+56945636 MS.gene004542:CDS
GGGTCGCAGGAAAGTAGCCA+TGG 0.602735 2.1:-56945304 None:intergenic
CAGGCTTCCAGCAAGAACCA+AGG 0.603768 2.1:-56945413 None:intergenic
ATCAGATTGCTAATTGAGAA+CGG 0.608545 2.1:+56945556 MS.gene004542:CDS
GGCCATTGCCAAAGTACCAA+GGG 0.609457 2.1:+56945900 MS.gene004542:CDS
GACAGGTGACATACCGAGGT+TGG 0.612646 2.1:+56945633 MS.gene004542:CDS
CCAAGGGGCTCTTACTGACA+AGG 0.613557 2.1:-56945396 None:intergenic
CGAGAAAACTATCATACCTA+GGG 0.613750 2.1:+56945939 MS.gene004542:CDS
CATTTGTAATGACTGATAAG+CGG 0.615213 2.1:+56946025 MS.gene004542:CDS
ATTACGTCATATGATTCTCA+AGG 0.616147 2.1:+56945279 None:intergenic
ATACCTTTAAGAAATGGGGT+TGG 0.619145 2.1:+56945761 MS.gene004542:CDS
TATTGCACAACTGAGGCCCT+AGG 0.619185 2.1:-56945955 None:intergenic
GAATCTATAAACCAATTCGT+AGG 0.623076 2.1:-56945455 None:intergenic
GCTGAATCTTGCACACACCA+AGG 0.638715 2.1:+56946116 MS.gene004542:CDS
TGGCCATTGCCAAAGTACCA+AGG 0.638748 2.1:+56945899 MS.gene004542:CDS
TAGAAGATATTGCACAACTG+AGG 0.657378 2.1:-56945962 None:intergenic
TCCGGACAGGTGACATACCG+AGG 0.668311 2.1:+56945629 MS.gene004542:CDS
ATATGATTCTCAAGGCACCA+TGG 0.688804 2.1:+56945287 None:intergenic
GGCTTCCAGCAAGAACCAAG+GGG 0.719885 2.1:-56945411 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTAATTTTATCAA+TGG - chr2.1:56945842-56945861 None:intergenic 15.0%
!!! ATAACAAAAGATGTTTTTGA+AGG - chr2.1:56945688-56945707 None:intergenic 20.0%
!!! TAATTTAGTGATGAACTTTT+GGG + chr2.1:56945852-56945871 MS.gene004542:CDS 20.0%
!!! TTAATTTAGTGATGAACTTT+TGG + chr2.1:56945851-56945870 MS.gene004542:CDS 20.0%
! AACGTATTATGTATGTGTTA+AGG - chr2.1:56946053-56946072 None:intergenic 25.0%
! ATTCAAAATTCACATCTCTT+TGG + chr2.1:56945511-56945530 MS.gene004542:CDS 25.0%
! CAACTATAAACAAAACCTTA+TGG + chr2.1:56945725-56945744 MS.gene004542:CDS 25.0%
! GAGAGAAAAAATTGAAACTT+TGG - chr2.1:56945346-56945365 None:intergenic 25.0%
!! CACATACATAATACGTTTTA+AGG + chr2.1:56946056-56946075 MS.gene004542:CDS 25.0%
!!! ATACATAATACGTTTTAAGG+AGG + chr2.1:56946059-56946078 MS.gene004542:CDS 25.0%
!!! ATGGATTTTAACATACAATG+AGG - chr2.1:56945823-56945842 None:intergenic 25.0%
!!! GGTTTTGTTTATAGTTGTTT+TGG - chr2.1:56945722-56945741 None:intergenic 25.0%
!!! TGGATTTTAACATACAATGA+GGG - chr2.1:56945822-56945841 None:intergenic 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG + chr2.1:56945349-56945368 MS.gene004542:CDS 25.0%
ATCAGATTGCTAATTGAGAA+CGG + chr2.1:56945556-56945575 MS.gene004542:CDS 30.0%
ATCTTCTAACAAAAGGTTTG+CGG + chr2.1:56945977-56945996 MS.gene004542:CDS 30.0%
CATTTGTAATGACTGATAAG+CGG + chr2.1:56946025-56946044 MS.gene004542:CDS 30.0%
GAATCTATAAACCAATTCGT+AGG - chr2.1:56945458-56945477 None:intergenic 30.0%
GTGCAATATCTTCTAACAAA+AGG + chr2.1:56945970-56945989 MS.gene004542:CDS 30.0%
TATAAACCAATTCGTAGGTT+GGG - chr2.1:56945453-56945472 None:intergenic 30.0%
! AAGTCGATACCTTTAAGAAA+TGG + chr2.1:56945755-56945774 MS.gene004542:CDS 30.0%
! AGTCGATACCTTTAAGAAAT+GGG + chr2.1:56945756-56945775 MS.gene004542:CDS 30.0%
! TATTGCTGAAGCATTTAGAA+AGG + chr2.1:56945795-56945814 MS.gene004542:CDS 30.0%
ACTAATAACCCTTGGTACTT+TGG - chr2.1:56945911-56945930 None:intergenic 35.0%
AGACTACAACTAATAACCCT+TGG - chr2.1:56945919-56945938 None:intergenic 35.0%
CGAGAAAACTATCATACCTA+GGG + chr2.1:56945939-56945958 MS.gene004542:CDS 35.0%
CGCTTATCAGTCATTACAAA+TGG - chr2.1:56946027-56946046 None:intergenic 35.0%
CTATAAACCAATTCGTAGGT+TGG - chr2.1:56945454-56945473 None:intergenic 35.0%
TAGAAGATATTGCACAACTG+AGG - chr2.1:56945965-56945984 None:intergenic 35.0%
TCGAGAAAACTATCATACCT+AGG + chr2.1:56945938-56945957 MS.gene004542:CDS 35.0%
TGGAGGAATTAAAGGATTTG+AGG + chr2.1:56945656-56945675 MS.gene004542:CDS 35.0%
! ATACCTTTAAGAAATGGGGT+TGG + chr2.1:56945761-56945780 MS.gene004542:CDS 35.0%
! ATCGACTTTCTCTTTCCATA+AGG - chr2.1:56945743-56945762 None:intergenic 35.0%
! GTCGATACCTTTAAGAAATG+GGG + chr2.1:56945757-56945776 MS.gene004542:CDS 35.0%
! TTGGGACTAAAAGATTTTCC+AGG - chr2.1:56945435-56945454 None:intergenic 35.0%
!! AACTTTTGGGTCAATCAGTT+GGG + chr2.1:56945865-56945884 MS.gene004542:CDS 35.0%
AAACATGGCAAGATTGCTTC+GGG + chr2.1:56945591-56945610 MS.gene004542:CDS 40.0%
AGACCAACCCCATTTCTTAA+AGG - chr2.1:56945767-56945786 None:intergenic 40.0%
CAAACATGGCAAGATTGCTT+CGG + chr2.1:56945590-56945609 MS.gene004542:CDS 40.0%
CAATCTTGCCATGTTTGAGT+CGG - chr2.1:56945587-56945606 None:intergenic 40.0%
TTTAATTCCTCCACCAACCT+CGG - chr2.1:56945649-56945668 None:intergenic 40.0%
TTTAGTCCCAACCTACGAAT+TGG + chr2.1:56945444-56945463 MS.gene004542:CDS 40.0%
! AAACTTTGGCAACACCCTTT+TGG - chr2.1:56945332-56945351 None:intergenic 40.0%
!! GAACTTTTGGGTCAATCAGT+TGG + chr2.1:56945864-56945883 MS.gene004542:CDS 40.0%
AACCCTTGGTACTTTGGCAA+TGG - chr2.1:56945905-56945924 None:intergenic 45.0%
TTGGGTCAATCAGTTGGGTT+GGG + chr2.1:56945870-56945889 MS.gene004542:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG + chr2.1:56945869-56945888 MS.gene004542:CDS 45.0%
! TTTGGCAACACCCTTTTGGA+GGG - chr2.1:56945328-56945347 None:intergenic 45.0%
!! GAGGTTGGTGGAGGAATTAA+AGG + chr2.1:56945648-56945667 MS.gene004542:CDS 45.0%
!! GTCAGAAGTAGAAGCACCTT+TGG - chr2.1:56945368-56945387 None:intergenic 45.0%
AGGCTTCCAGCAAGAACCAA+GGG - chr2.1:56945415-56945434 None:intergenic 50.0%
GCTGAATCTTGCACACACCA+AGG + chr2.1:56946116-56946135 MS.gene004542:CDS 50.0%
TATTGCACAACTGAGGCCCT+AGG - chr2.1:56945958-56945977 None:intergenic 50.0%
! CTTTGGCAACACCCTTTTGG+AGG - chr2.1:56945329-56945348 None:intergenic 50.0%
! GGCCATTGCCAAAGTACCAA+GGG + chr2.1:56945900-56945919 MS.gene004542:CDS 50.0%
! TGGCCATTGCCAAAGTACCA+AGG + chr2.1:56945899-56945918 MS.gene004542:CDS 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG + chr2.1:56945879-56945898 MS.gene004542:CDS 50.0%
!! TTGGCAACACCCTTTTGGAG+GGG - chr2.1:56945327-56945346 None:intergenic 50.0%
ACCTCGGTATGTCACCTGTC+CGG - chr2.1:56945633-56945652 None:intergenic 55.0%
AGGTGACATACCGAGGTTGG+TGG + chr2.1:56945636-56945655 MS.gene004542:CDS 55.0%
ATTGCTTCGGGACTGGAGTC+AGG + chr2.1:56945603-56945622 MS.gene004542:CDS 55.0%
CAGGCTTCCAGCAAGAACCA+AGG - chr2.1:56945416-56945435 None:intergenic 55.0%
CCAAGGGGCTCTTACTGACA+AGG - chr2.1:56945399-56945418 None:intergenic 55.0%
CCTTGTCAGTAAGAGCCCCT+TGG + chr2.1:56945396-56945415 MS.gene004542:CDS 55.0%
GACAGGTGACATACCGAGGT+TGG + chr2.1:56945633-56945652 MS.gene004542:CDS 55.0%
GGCTTCCAGCAAGAACCAAG+GGG - chr2.1:56945414-56945433 None:intergenic 55.0%
GGGACTGGAGTCAGGTATTC+CGG + chr2.1:56945611-56945630 MS.gene004542:CDS 55.0%
TGGAGTCAGGTATTCCGGAC+AGG + chr2.1:56945616-56945635 MS.gene004542:CDS 55.0%
TGGCAAGATTGCTTCGGGAC+TGG + chr2.1:56945596-56945615 MS.gene004542:CDS 55.0%
TTCCTGCGACCCCTCCAAAA+GGG + chr2.1:56945315-56945334 MS.gene004542:CDS 55.0%
TTTCCTGCGACCCCTCCAAA+AGG + chr2.1:56945314-56945333 MS.gene004542:CDS 55.0%
! AAGAGCCCCTTGGTTCTTGC+TGG + chr2.1:56945406-56945425 MS.gene004542:CDS 55.0%
! CGGAGTGACCGACTCAAACA+TGG + chr2.1:56945576-56945595 MS.gene004542:CDS 55.0%
! CTTGGTTCTTGCTGGAAGCC+TGG + chr2.1:56945414-56945433 MS.gene004542:CDS 55.0%
!! TGACATACCGAGGTTGGTGG+AGG + chr2.1:56945639-56945658 MS.gene004542:CDS 55.0%
GGGTCGCAGGAAAGTAGCCA+TGG - chr2.1:56945307-56945326 None:intergenic 60.0%
TCCGGACAGGTGACATACCG+AGG + chr2.1:56945629-56945648 MS.gene004542:CDS 60.0%
!! CACCCTTTTGGAGGGGTCGC+AGG - chr2.1:56945320-56945339 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 56945289 56946143 56945289 ID=MS.gene004542
chr2.1 mRNA 56945289 56946143 56945289 ID=MS.gene004542.t1;Parent=MS.gene004542
chr2.1 exon 56945289 56946143 56945289 ID=MS.gene004542.t1.exon1;Parent=MS.gene004542.t1
chr2.1 CDS 56945289 56946143 56945289 ID=cds.MS.gene004542.t1;Parent=MS.gene004542.t1
Gene Sequence

>MS.gene004542

ATGATTCTCAAGGCACCATGGCTACTTTCCTGCGACCCCTCCAAAAGGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACTTCTGACATTGTTAACCTTGTCAGTAAGAGCCCCTTGGTTCTTGCTGGAAGCCTGGAAAATCTTTTAGTCCCAACCTACGAATTGGTTTATAGATTCTTGCAATCTCACGATGACACTATTGCTTGTGTAATTCAAAATTCACATCTCTTTGGACAGAGTAATGTGTCATCTAATATCAGATTGCTAATTGAGAACGGAGTGACCGACTCAAACATGGCAAGATTGCTTCGGGACTGGAGTCAGGTATTCCGGACAGGTGACATACCGAGGTTGGTGGAGGAATTAAAGGATTTGAGGTTTAATCCTTCAAAAACATCTTTTGTTATAGCGCTGCTTGCCAAAACAACTATAAACAAAACCTTATGGAAAGAGAAAGTCGATACCTTTAAGAAATGGGGTTGGTCTGATGAAGATATTGCTGAAGCATTTAGAAAGGACCCTCATTGTATGTTAAAATCCATTGATAAAATTAATTTAGTGATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAAGTACCAAGGGTTATTAGTTGTAGTCTCGAGAAAACTATCATACCTAGGGCCTCAGTTGTGCAATATCTTCTAACAAAAGGTTTGCGGAAAAAGAATGCAAGCTTAACTACTCCATTTGTAATGACTGATAAGCGGTTCCTTAACACATACATAATACGTTTTAAGGAGGAGTCATCTTATCTCTTAAAGCTCTATGAAGAAAAGCTGAATCTTGCACACACCAAGGACTAA
Protein sequence

>MS.gene004542.t1

MILKAPWLLSCDPSKRVLPKFQFFLSKGASTSDIVNLVSKSPLVLAGSLENLLVPTYELVYRFLQSHDDTIACVIQNSHLFGQSNVSSNIRLLIENGVTDSNMARLLRDWSQVFRTGDIPRLVEELKDLRFNPSKTSFVIALLAKTTINKTLWKEKVDTFKKWGWSDEDIAEAFRKDPHCMLKSIDKINLVMNFWVNQLGWDAMAIAKVPRVISCSLEKTIIPRASVVQYLLTKGLRKKNASLTTPFVMTDKRFLNTYIIRFKEESSYLLKLYEEKLNLAHTKD