AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004584

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004584.t1 MTR_2g437060 89.620 395 39 1 1 393 1 395 0.0 719
MS.gene004584.t1 MTR_2g437080 76.302 384 81 2 9 392 6 379 0.0 588
MS.gene004584.t1 MTR_2g436400 76.093 389 83 3 1 387 1 381 0.0 587
MS.gene004584.t1 MTR_2g437020 67.342 395 112 4 1 393 1 380 0.0 524
MS.gene004584.t1 MTR_2g436440 66.158 393 124 4 1 392 1 385 0.0 511
MS.gene004584.t1 MTR_2g437030 66.839 386 124 2 9 393 84 466 8.34e-180 508
MS.gene004584.t1 MTR_2g437040 64.888 356 125 0 38 393 25 380 2.61e-166 471
MS.gene004584.t1 MTR_2g437160 52.044 367 169 3 31 391 38 403 3.27e-127 372
MS.gene004584.t1 MTR_2g437170 51.169 385 177 6 18 391 14 398 1.08e-126 371
MS.gene004584.t1 MTR_2g437120 53.552 366 164 4 31 391 27 391 1.39e-126 371
MS.gene004584.t1 MTR_4g119550 52.754 345 160 2 49 392 47 389 1.59e-126 370
MS.gene004584.t1 MTR_2g437150 50.785 382 177 6 18 388 14 395 3.28e-124 365
MS.gene004584.t1 MTR_2g437200 50.392 383 148 4 41 383 18 398 1.93e-123 362
MS.gene004584.t1 MTR_2g437960 52.047 342 161 2 55 393 55 396 7.88e-122 358
MS.gene004584.t1 MTR_2g437960 49.471 378 183 3 24 393 19 396 1.36e-121 359
MS.gene004584.t1 MTR_2g437990 50.275 364 172 3 36 391 28 390 4.18e-120 355
MS.gene004584.t1 MTR_2g437260 49.288 351 172 4 41 387 22 370 8.45e-120 353
MS.gene004584.t1 MTR_2g076320 50.000 366 163 4 36 387 28 387 7.77e-119 351
MS.gene004584.t1 MTR_2g438010 50.829 362 166 5 36 387 48 407 2.17e-118 350
MS.gene004584.t1 MTR_2g437180 47.644 382 192 4 14 391 11 388 1.07e-117 348
MS.gene004584.t1 MTR_2g437130 49.587 363 179 2 29 391 21 379 1.19e-117 347
MS.gene004584.t1 MTR_2g437100 51.630 368 163 6 31 383 28 395 3.37e-115 342
MS.gene004584.t1 MTR_2g437240 46.402 403 170 5 27 391 12 406 9.85e-115 341
MS.gene004584.t1 MTR_2g438020 47.090 378 160 3 52 391 70 445 2.99e-110 332
MS.gene004584.t1 MTR_2g437940 49.315 365 152 6 31 391 27 362 5.01e-110 327
MS.gene004584.t1 MTR_2g436460 46.802 344 180 2 44 386 38 379 2.30e-104 313
MS.gene004584.t1 MTR_2g436370 71.569 204 47 4 1 202 1 195 3.06e-92 276
MS.gene004584.t1 MTR_4g120380 42.049 371 206 3 11 381 9 370 2.04e-91 280
MS.gene004584.t1 MTR_2g019840 46.687 332 171 4 54 383 44 371 1.22e-90 278
MS.gene004584.t1 MTR_4g119580 42.522 341 192 2 43 381 32 370 6.50e-90 276
MS.gene004584.t1 MTR_2g019810 44.880 332 177 4 54 383 32 359 3.66e-87 269
MS.gene004584.t1 MTR_4g120380 43.020 351 191 3 11 361 9 350 8.22e-87 270
MS.gene004584.t1 MTR_2g437090 64.881 168 59 0 226 393 1 168 1.19e-76 235
MS.gene004584.t1 MTR_2g437160 56.219 201 85 1 194 391 112 312 2.18e-72 229
MS.gene004584.t1 MTR_3g450500 32.440 336 215 4 54 381 39 370 2.42e-52 179
MS.gene004584.t1 MTR_4g119570 35.743 249 127 1 133 381 14 229 1.41e-45 157
MS.gene004584.t1 MTR_4g007490 32.022 356 232 7 33 381 16 368 2.29e-45 160
MS.gene004584.t1 MTR_8g080990 29.831 295 204 2 86 377 74 368 4.10e-34 130
MS.gene004584.t1 MTR_8g081000 26.409 337 244 3 48 381 50 385 4.10e-29 117
MS.gene004584.t1 MTR_8g081000 26.409 337 244 3 48 381 50 385 5.20e-29 117
MS.gene004584.t1 MTR_8g081000 26.409 337 244 3 48 381 50 385 8.84e-29 116
MS.gene004584.t1 MTR_2g436380 53.535 99 43 2 226 324 1 96 2.72e-24 101
MS.gene004584.t1 MTR_4g049400 43.307 127 67 2 33 154 25 151 1.43e-23 98.6
MS.gene004584.t1 MTR_3g085240 37.584 149 83 3 27 175 32 170 4.82e-20 89.0
MS.gene004584.t1 MTR_3g085240 46.067 89 31 1 285 373 176 247 1.24e-16 79.3
MS.gene004584.t1 MTR_8g012210 22.145 289 216 7 99 381 63 348 6.23e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004584.t1 AT5G07900 36.036 333 205 4 54 380 69 399 1.56e-68 222
MS.gene004584.t1 AT1G21150 30.514 331 222 4 54 378 56 384 5.23e-48 168
MS.gene004584.t1 AT1G21150 29.619 341 232 4 44 378 10 348 5.41e-48 167
MS.gene004584.t1 AT1G21150 30.514 331 222 4 54 378 120 448 2.33e-47 167
MS.gene004584.t1 AT1G61980 28.455 369 219 8 54 381 52 416 2.01e-34 132
MS.gene004584.t1 AT1G61980 28.455 369 219 8 54 381 52 416 2.01e-34 132
MS.gene004584.t1 AT1G61980 28.455 369 219 8 54 381 52 416 2.01e-34 132
MS.gene004584.t1 AT1G61970 27.841 352 209 6 55 366 53 399 8.50e-32 125
MS.gene004584.t1 AT1G61970 27.841 352 209 6 55 366 53 399 8.50e-32 125
MS.gene004584.t1 AT1G61970 27.841 352 209 6 55 366 53 399 8.50e-32 125
MS.gene004584.t1 AT1G61970 27.841 352 209 6 55 366 53 399 8.50e-32 125
MS.gene004584.t1 AT1G61970 27.841 352 209 6 55 366 53 399 8.50e-32 125
MS.gene004584.t1 AT1G61970 27.841 352 209 6 55 366 53 399 8.50e-32 125
MS.gene004584.t1 AT1G61970 27.841 352 209 6 55 366 53 399 8.50e-32 125
MS.gene004584.t1 AT1G62120 28.691 359 203 9 55 368 57 407 9.79e-32 125
MS.gene004584.t1 AT5G64950 28.691 359 233 8 44 388 26 375 1.38e-31 124
MS.gene004584.t1 AT1G61990 27.324 355 206 7 55 368 53 396 2.07e-30 121
MS.gene004584.t1 AT1G61990 27.324 355 206 7 55 368 53 396 2.07e-30 121
MS.gene004584.t1 AT1G62085 27.792 403 195 12 27 342 21 414 7.74e-30 120
MS.gene004584.t1 AT1G62085 27.792 403 195 12 27 342 21 414 7.74e-30 120
MS.gene004584.t1 AT3G46950 31.086 267 173 5 37 298 31 291 2.02e-28 116
MS.gene004584.t1 AT5G23930 27.436 390 203 10 54 371 55 436 8.61e-27 111
MS.gene004584.t1 AT1G62110 27.891 294 192 5 8 296 17 295 2.63e-24 104
MS.gene004584.t1 AT1G61960 25.269 372 192 8 55 348 53 416 8.36e-23 100
MS.gene004584.t1 AT1G62010 24.573 293 183 3 87 343 69 359 9.07e-22 96.7
MS.gene004584.t1 AT1G62150 24.000 375 198 9 48 343 52 418 4.46e-21 95.1

Find 84 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATACCTCATTCACAATTT+TGG 0.061494 2.2:-52166005 MS.gene004584:CDS
GCAATCTGACAAGGAAATTT+TGG 0.125855 2.2:-52165664 MS.gene004584:CDS
GGAAAAGAGGGTTAGGGTTT+AGG 0.191466 2.2:+52166096 None:intergenic
CGACCAACCCCATTTCTTAA+AGG 0.207846 2.2:+52165380 None:intergenic
GTGAAGGAATTAAAGGATTT+GGG 0.210460 2.2:-52165489 MS.gene004584:CDS
GAAAAGAGGGTTAGGGTTTA+GGG 0.220235 2.2:+52166097 None:intergenic
GGTGAAGGAATTAAAGGATT+TGG 0.236355 2.2:-52165490 MS.gene004584:CDS
TTAATTTAGTGATGAGCTTT+TGG 0.242008 2.2:-52165293 MS.gene004584:CDS
TGACAAAATATCAGGTGTTT+TGG 0.255478 2.2:+52165236 None:intergenic
GTGCAATTTCTTCTCAATAA+TGG 0.259750 2.2:-52165174 MS.gene004584:CDS
TAATCCCTTGAGATAGATTA+AGG 0.266032 2.2:+52166142 None:intergenic
CAAGTTGGTGAAGGAATTAA+AGG 0.289341 2.2:-52165496 MS.gene004584:CDS
TTTCTCATTCCACAAGGTTT+TGG 0.304420 2.2:+52165410 None:intergenic
TTATCTTATAAAGTTGTATC+AGG 0.309342 2.2:-52165055 MS.gene004584:CDS
TGAGAACTGAATCAGGGTTT+TGG 0.309396 2.2:+52165922 None:intergenic
AAACTTTGGCAACACCCTTT+TGG 0.315392 2.2:+52165815 None:intergenic
CTTGTAACTGGAGCAAAGTT+TGG 0.321987 2.2:+52165962 None:intergenic
AAGAAATTGAGAACTGAATC+AGG 0.327189 2.2:+52165915 None:intergenic
CTTGAAACGGAGCTTGTAAC+TGG 0.364053 2.2:+52165950 None:intergenic
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.2:-52165274 MS.gene004584:CDS
AAGTTGATGCCTTTAAGAAA+TGG 0.366199 2.2:-52165389 MS.gene004584:CDS
AGTTGATGCCTTTAAGAAAT+GGG 0.371481 2.2:-52165388 MS.gene004584:CDS
AAACTTAATGACAAAATATC+AGG 0.378240 2.2:+52165228 None:intergenic
AAATGGAGAGAAAAGAGAAA+TGG 0.389921 2.2:+52166065 None:intergenic
CAGAAGCGTTGGTGCAGAAA+TGG 0.394609 2.2:+52166048 None:intergenic
GCTTAATTTATCCATTCTTA+AGG 0.397953 2.2:-52165131 MS.gene004584:CDS
CAAACATTAAAAGATTGCTT+AGG 0.402903 2.2:-52165554 MS.gene004584:CDS
GTCAGAAGTAGAAGCGCCTT+TGG 0.405353 2.2:+52165779 None:intergenic
AATGGAGAGAAAAGAGAAAT+GGG 0.410398 2.2:+52166066 None:intergenic
AAAAGAGAAATGGGTAATGT+TGG 0.413194 2.2:+52166075 None:intergenic
AATAGTCCCAACCTATGAAT+TGG 0.421560 2.2:-52165700 MS.gene004584:CDS
TAATGTTGGAAAAGAGGGTT+AGG 0.425634 2.2:+52166089 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2.2:-52165275 MS.gene004584:CDS
GGAAGTAGCCATGGTGCCTT+GGG 0.435586 2.2:+52165849 None:intergenic
GGGAAGTAGCCATGGTGCCT+TGG 0.446977 2.2:+52165848 None:intergenic
AAACATTAAAAGATTGCTTA+GGG 0.465201 2.2:-52165553 MS.gene004584:CDS
TTCCCTGCGACCCCTCCAAA+AGG 0.466368 2.2:-52165830 MS.gene004584:CDS
CTCAATTTCTTTAGAAACCA+TGG 0.466986 2.2:-52165903 MS.gene004584:CDS
GGGAATGATACGACGTAGTT+GGG 0.467688 2.2:+52165869 None:intergenic
AGGCTTACAGACAAGACGCC+AGG 0.474816 2.2:+52165732 None:intergenic
GAATCTGTACACCAATTCAT+AGG 0.478001 2.2:+52165689 None:intergenic
CTGTACACCAATTCATAGGT+TGG 0.484595 2.2:+52165693 None:intergenic
ATCACAATGCTGATTGAGAA+TGG 0.486666 2.2:-52165588 MS.gene004584:CDS
TGGGAATGATACGACGTAGT+TGG 0.489187 2.2:+52165868 None:intergenic
CAAAGTTTGGAAGCAGATTG+TGG 0.492532 2.2:+52165975 None:intergenic
ATGGGTAATGTTGGAAAAGA+GGG 0.501669 2.2:+52166084 None:intergenic
TGTACACCAATTCATAGGTT+GGG 0.507173 2.2:+52165694 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.508902 2.2:-52165265 MS.gene004584:CDS
TTGGGACTATTTGATTCTTG+AGG 0.509547 2.2:+52165712 None:intergenic
AAAGTTTGGAAGCAGATTGT+GGG 0.511078 2.2:+52165976 None:intergenic
AATGGGTAATGTTGGAAAAG+AGG 0.520798 2.2:+52166083 None:intergenic
AACTTCTCAGACCTTAAGAA+TGG 0.523239 2.2:+52165120 None:intergenic
GGGTCGCAGGGAAGTAGCCA+TGG 0.532044 2.2:+52165840 None:intergenic
AACTCAAGCTTGCATACACC+AGG 0.537467 2.2:-52165029 MS.gene004584:CDS
GAGGTATGAGACAGCAAAAG+AGG 0.548116 2.2:+52166019 None:intergenic
AGAGGTTGAATCAGAAGCGT+TGG 0.556682 2.2:+52166037 None:intergenic
CGCCAGGGTTCTTACACACA+AGG 0.557000 2.2:+52165748 None:intergenic
TCCCTGCGACCCCTCCAAAA+GGG 0.564822 2.2:-52165829 MS.gene004584:CDS
CTTTAAGAAATGGGGTTGGT+CGG 0.566588 2.2:-52165379 MS.gene004584:CDS
CGAGCCCAAAATTGTGAATG+AGG 0.569173 2.2:+52166000 None:intergenic
GTTGATGCCTTTAAGAAATG+GGG 0.573670 2.2:-52165387 MS.gene004584:CDS
CAGATTCTTGCAATCTGACA+AGG 0.575407 2.2:-52165673 MS.gene004584:CDS
TGTTCAAGTTCAACAATACA+TGG 0.576396 2.2:-52166175 MS.gene004584:CDS
CAGAACCTTAATCTATCTCA+AGG 0.578893 2.2:-52166147 MS.gene004584:CDS
TGAAGGAATTAAAGGATTTG+GGG 0.584499 2.2:-52165488 MS.gene004584:CDS
TGACAATCATGTCTTGTCCC+TGG 0.585106 2.2:+52165011 None:intergenic
GACACGAGACATGCTCAAGT+TGG 0.588113 2.2:-52165511 MS.gene004584:CDS
AGAAATTGAGAACTGAATCA+GGG 0.588690 2.2:+52165916 None:intergenic
AATTGCACAACAGTGGCTCG+TGG 0.602758 2.2:+52165189 None:intergenic
ATGCCTTTAAGAAATGGGGT+TGG 0.606718 2.2:-52165383 MS.gene004584:CDS
TGAGATAGATTAAGGTTCTG+TGG 0.615734 2.2:+52166150 None:intergenic
GTTGGGAGTTAGAGAAACCA+TGG 0.615746 2.2:+52165886 None:intergenic
CATCAACAACCAAAACCTTG+TGG 0.615914 2.2:-52165419 MS.gene004584:CDS
AGACATGCTCAAGTTGGTGA+AGG 0.618045 2.2:-52165505 MS.gene004584:CDS
AATGTTGGAAAAGAGGGTTA+GGG 0.620954 2.2:+52166090 None:intergenic
GGCTTACAGACAAGACGCCA+GGG 0.643701 2.2:+52165733 None:intergenic
ATCAACTTTCTCATTCCACA+AGG 0.645223 2.2:+52165404 None:intergenic
GAGAAGAAATTGCACAACAG+TGG 0.646434 2.2:+52165182 None:intergenic
AGAACCTTAATCTATCTCAA+GGG 0.657921 2.2:-52166146 MS.gene004584:CDS
AACCTTGTGTGTAAGAACCC+TGG 0.659854 2.2:-52165750 MS.gene004584:CDS
ACTACGTCGTATCATTCCCA+AGG 0.661929 2.2:-52165865 MS.gene004584:CDS
GTATCATTCCCAAGGCACCA+TGG 0.712393 2.2:-52165857 MS.gene004584:CDS
ATGGATGTTATCATACAGTG+AGG 0.724940 2.2:+52165324 None:intergenic
ACTCAAGCTTGCATACACCA+GGG 0.735651 2.2:-52165028 MS.gene004584:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTAATTTTATCTA+TGG + chr2.2:52165891-52165910 None:intergenic 15.0%
!! AAACATTAAAAGATTGCTTA+GGG - chr2.2:52165640-52165659 MS.gene004584:CDS 20.0%
!! AAACTTAATGACAAAATATC+AGG + chr2.2:52165968-52165987 None:intergenic 20.0%
!! TTATCTTATAAAGTTGTATC+AGG - chr2.2:52166138-52166157 MS.gene004584:CDS 20.0%
!!! AATCATTCAAAAACAACTTT+TGG - chr2.2:52165731-52165750 MS.gene004584:CDS 20.0%
!!! TGATATTTTGTCATTAAGTT+TGG - chr2.2:52165967-52165986 MS.gene004584:CDS 20.0%
! CAAACATTAAAAGATTGCTT+AGG - chr2.2:52165639-52165658 MS.gene004584:CDS 25.0%
! GAGAGAAAAAATTGAAACTT+TGG + chr2.2:52165395-52165414 None:intergenic 25.0%
! GCTTAATTTATCCATTCTTA+AGG - chr2.2:52166062-52166081 MS.gene004584:CDS 25.0%
! TGTCATTAAGTTTGGAAAAA+AGG - chr2.2:52165975-52165994 MS.gene004584:CDS 25.0%
!! CAATCTTTTAATGTTTGAGT+GGG + chr2.2:52165636-52165655 None:intergenic 25.0%
!! TAATTTAGTGATGAGCTTTT+GGG - chr2.2:52165901-52165920 MS.gene004584:CDS 25.0%
!! TTAATTTAGTGATGAGCTTT+TGG - chr2.2:52165900-52165919 MS.gene004584:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG - chr2.2:52165398-52165417 MS.gene004584:CDS 25.0%
AAAAGAGAAATGGGTAATGT+TGG + chr2.2:52165121-52165140 None:intergenic 30.0%
AAATGGAGAGAAAAGAGAAA+TGG + chr2.2:52165131-52165150 None:intergenic 30.0%
AAGAAATTGAGAACTGAATC+AGG + chr2.2:52165281-52165300 None:intergenic 30.0%
AATGGAGAGAAAAGAGAAAT+GGG + chr2.2:52165130-52165149 None:intergenic 30.0%
AGAAATTGAGAACTGAATCA+GGG + chr2.2:52165280-52165299 None:intergenic 30.0%
AGAACCTTAATCTATCTCAA+GGG - chr2.2:52165047-52165066 MS.gene004584:CDS 30.0%
GTGAAGGAATTAAAGGATTT+GGG - chr2.2:52165704-52165723 MS.gene004584:CDS 30.0%
GTGCAATTTCTTCTCAATAA+TGG - chr2.2:52166019-52166038 MS.gene004584:CDS 30.0%
TAATCCCTTGAGATAGATTA+AGG + chr2.2:52165054-52165073 None:intergenic 30.0%
TGAAGGAATTAAAGGATTTG+GGG - chr2.2:52165705-52165724 MS.gene004584:CDS 30.0%
! AGTTGATGCCTTTAAGAAAT+GGG - chr2.2:52165805-52165824 MS.gene004584:CDS 30.0%
! CATACCTCATTCACAATTTT+GGG - chr2.2:52165189-52165208 MS.gene004584:CDS 30.0%
! CTCAATTTCTTTAGAAACCA+TGG - chr2.2:52165290-52165309 MS.gene004584:CDS 30.0%
! GCAATCTTTTAATGTTTGAG+TGG + chr2.2:52165637-52165656 None:intergenic 30.0%
! TCATACCTCATTCACAATTT+TGG - chr2.2:52165188-52165207 MS.gene004584:CDS 30.0%
! TGACAAAATATCAGGTGTTT+TGG + chr2.2:52165960-52165979 None:intergenic 30.0%
! TGTTCAAGTTCAACAATACA+TGG - chr2.2:52165018-52165037 MS.gene004584:CDS 30.0%
!! AAGTTGATGCCTTTAAGAAA+TGG - chr2.2:52165804-52165823 MS.gene004584:CDS 30.0%
AAAGTTTGGAAGCAGATTGT+GGG + chr2.2:52165220-52165239 None:intergenic 35.0%
AACTTCTCAGACCTTAAGAA+TGG + chr2.2:52166076-52166095 None:intergenic 35.0%
AATAGTCCCAACCTATGAAT+TGG - chr2.2:52165493-52165512 MS.gene004584:CDS 35.0%
AATGGGTAATGTTGGAAAAG+AGG + chr2.2:52165113-52165132 None:intergenic 35.0%
AATGTTGGAAAAGAGGGTTA+GGG + chr2.2:52165106-52165125 None:intergenic 35.0%
ATCAACTTTCTCATTCCACA+AGG + chr2.2:52165792-52165811 None:intergenic 35.0%
ATGGATGTTATCATACAGTG+AGG + chr2.2:52165872-52165891 None:intergenic 35.0%
ATGGGTAATGTTGGAAAAGA+GGG + chr2.2:52165112-52165131 None:intergenic 35.0%
CAGAACCTTAATCTATCTCA+AGG - chr2.2:52165046-52165065 MS.gene004584:CDS 35.0%
GAATCTGTACACCAATTCAT+AGG + chr2.2:52165507-52165526 None:intergenic 35.0%
GGTGAAGGAATTAAAGGATT+TGG - chr2.2:52165703-52165722 MS.gene004584:CDS 35.0%
TAATGTTGGAAAAGAGGGTT+AGG + chr2.2:52165107-52165126 None:intergenic 35.0%
TGAGATAGATTAAGGTTCTG+TGG + chr2.2:52165046-52165065 None:intergenic 35.0%
TGTACACCAATTCATAGGTT+GGG + chr2.2:52165502-52165521 None:intergenic 35.0%
TTGGGACTATTTGATTCTTG+AGG + chr2.2:52165484-52165503 None:intergenic 35.0%
! ATCACAATGCTGATTGAGAA+TGG - chr2.2:52165605-52165624 MS.gene004584:CDS 35.0%
! GCAATCTGACAAGGAAATTT+TGG - chr2.2:52165529-52165548 MS.gene004584:CDS 35.0%
! GTTGATGCCTTTAAGAAATG+GGG - chr2.2:52165806-52165825 MS.gene004584:CDS 35.0%
! GTTTAGGGATTGCGATTTTT+GGG + chr2.2:52165084-52165103 None:intergenic 35.0%
! TTTAGGGATTGCGATTTTTG+GGG + chr2.2:52165083-52165102 None:intergenic 35.0%
! TTTCTCATTCCACAAGGTTT+TGG + chr2.2:52165786-52165805 None:intergenic 35.0%
!! CAAGTTGGTGAAGGAATTAA+AGG - chr2.2:52165697-52165716 MS.gene004584:CDS 35.0%
!!! AATATCAGGTGTTTTGGCAA+CGG + chr2.2:52165954-52165973 None:intergenic 35.0%
CAAAGTTTGGAAGCAGATTG+TGG + chr2.2:52165221-52165240 None:intergenic 40.0%
CAGATTCTTGCAATCTGACA+AGG - chr2.2:52165520-52165539 MS.gene004584:CDS 40.0%
CATCAACAACCAAAACCTTG+TGG - chr2.2:52165774-52165793 MS.gene004584:CDS 40.0%
CTGTACACCAATTCATAGGT+TGG + chr2.2:52165503-52165522 None:intergenic 40.0%
CTTGTAACTGGAGCAAAGTT+TGG + chr2.2:52165234-52165253 None:intergenic 40.0%
GAAAAGAGGGTTAGGGTTTA+GGG + chr2.2:52165099-52165118 None:intergenic 40.0%
GAGAAGAAATTGCACAACAG+TGG + chr2.2:52166014-52166033 None:intergenic 40.0%
! AAACTTTGGCAACACCCTTT+TGG + chr2.2:52165381-52165400 None:intergenic 40.0%
! ATGCCTTTAAGAAATGGGGT+TGG - chr2.2:52165810-52165829 MS.gene004584:CDS 40.0%
! CTTTAAGAAATGGGGTTGGT+CGG - chr2.2:52165814-52165833 MS.gene004584:CDS 40.0%
! GGTTTAGGGATTGCGATTTT+TGG + chr2.2:52165085-52165104 None:intergenic 40.0%
! TGAGAACTGAATCAGGGTTT+TGG + chr2.2:52165274-52165293 None:intergenic 40.0%
!! AGCTTTTGGGTCAATCAGTT+GGG - chr2.2:52165914-52165933 MS.gene004584:CDS 40.0%
!! GGTTTTGGTGAGTCTTGAAA+CGG + chr2.2:52165259-52165278 None:intergenic 40.0%
!! TTAGGGATTGCGATTTTTGG+GGG + chr2.2:52165082-52165101 None:intergenic 40.0%
AACCTTGTGTGTAAGAACCC+TGG - chr2.2:52165443-52165462 MS.gene004584:CDS 45.0%
AACTCAAGCTTGCATACACC+AGG - chr2.2:52166164-52166183 MS.gene004584:CDS 45.0%
ACTACGTCGTATCATTCCCA+AGG - chr2.2:52165328-52165347 MS.gene004584:CDS 45.0%
ACTCAAGCTTGCATACACCA+GGG - chr2.2:52166165-52166184 MS.gene004584:CDS 45.0%
CGACCAACCCCATTTCTTAA+AGG + chr2.2:52165816-52165835 None:intergenic 45.0%
CGAGCCCAAAATTGTGAATG+AGG + chr2.2:52165196-52165215 None:intergenic 45.0%
CTTGAAACGGAGCTTGTAAC+TGG + chr2.2:52165246-52165265 None:intergenic 45.0%
GAGGTATGAGACAGCAAAAG+AGG + chr2.2:52165177-52165196 None:intergenic 45.0%
GGAAAAGAGGGTTAGGGTTT+AGG + chr2.2:52165100-52165119 None:intergenic 45.0%
GGGAATGATACGACGTAGTT+GGG + chr2.2:52165327-52165346 None:intergenic 45.0%
GTTGGGAGTTAGAGAAACCA+TGG + chr2.2:52165310-52165329 None:intergenic 45.0%
TGGGAATGATACGACGTAGT+TGG + chr2.2:52165328-52165347 None:intergenic 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.2:52165919-52165938 MS.gene004584:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.2:52165918-52165937 MS.gene004584:CDS 45.0%
! AGAGGTTGAATCAGAAGCGT+TGG + chr2.2:52165159-52165178 None:intergenic 45.0%
! TTTGGCAACACCCTTTTGGA+GGG + chr2.2:52165377-52165396 None:intergenic 45.0%
!! AGACATGCTCAAGTTGGTGA+AGG - chr2.2:52165688-52165707 MS.gene004584:CDS 45.0%
!! GAGCTTTTGGGTCAATCAGT+TGG - chr2.2:52165913-52165932 MS.gene004584:CDS 45.0%
AATTGCACAACAGTGGCTCG+TGG + chr2.2:52166007-52166026 None:intergenic 50.0%
GACACGAGACATGCTCAAGT+TGG - chr2.2:52165682-52165701 MS.gene004584:CDS 50.0%
! CTTTGGCAACACCCTTTTGG+AGG + chr2.2:52165378-52165397 None:intergenic 50.0%
! GTATCATTCCCAAGGCACCA+TGG - chr2.2:52165336-52165355 MS.gene004584:CDS 50.0%
!! CAGAAGCGTTGGTGCAGAAA+TGG + chr2.2:52165148-52165167 None:intergenic 50.0%
!! GTCAGAAGTAGAAGCGCCTT+TGG + chr2.2:52165417-52165436 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG - chr2.2:52165928-52165947 MS.gene004584:CDS 50.0%
!! TTGGCAACACCCTTTTGGAG+GGG + chr2.2:52165376-52165395 None:intergenic 50.0%
AGGCTTACAGACAAGACGCC+AGG + chr2.2:52165464-52165483 None:intergenic 55.0%
CGCCAGGGTTCTTACACACA+AGG + chr2.2:52165448-52165467 None:intergenic 55.0%
GGAAGTAGCCATGGTGCCTT+GGG + chr2.2:52165347-52165366 None:intergenic 55.0%
GGCTTACAGACAAGACGCCA+GGG + chr2.2:52165463-52165482 None:intergenic 55.0%
GGGAAGTAGCCATGGTGCCT+TGG + chr2.2:52165348-52165367 None:intergenic 60.0%
TCCCTGCGACCCCTCCAAAA+GGG - chr2.2:52165364-52165383 MS.gene004584:CDS 60.0%
TTCCCTGCGACCCCTCCAAA+AGG - chr2.2:52165363-52165382 MS.gene004584:CDS 60.0%
!! ACCCTTTTGGAGGGGTCGCA+GGG + chr2.2:52165368-52165387 None:intergenic 60.0%
GGGTCGCAGGGAAGTAGCCA+TGG + chr2.2:52165356-52165375 None:intergenic 65.0%
!! CACCCTTTTGGAGGGGTCGC+AGG + chr2.2:52165369-52165388 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 52165017 52166198 52165017 ID=MS.gene004584
chr2.2 mRNA 52165017 52166198 52165017 ID=MS.gene004584.t1;Parent=MS.gene004584
chr2.2 exon 52165017 52166198 52165017 ID=MS.gene004584.t1.exon1;Parent=MS.gene004584.t1
chr2.2 CDS 52165017 52166198 52165017 ID=cds.MS.gene004584.t1;Parent=MS.gene004584.t1
Gene Sequence

>MS.gene004584

ATGTTCAAGTTCAACAATACATGGCGCCACAGAACCTTAATCTATCTCAAGGGATTAACTTACCCCCAAAAATCGCAATCCCTAAACCCTAACCCTCTTTTCCAACATTACCCATTTCTCTTTTCTCTCCATTTCTGCACCAACGCTTCTGATTCAACCTCTTTTGCTGTCTCATACCTCATTCACAATTTTGGGCTCGACCCACAATCTGCTTCCAAACTTTGCTCCAGTTACAAGCTCCGTTTCAAGACTCACCAAAACCCTGATTCAGTTCTCAATTTCTTTAGAAACCATGGTTTCTCTAACTCCCAACTACGTCGTATCATTCCCAAGGCACCATGGCTACTTCCCTGCGACCCCTCCAAAAGGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGCGCTTCTACTTCTGACATTGTTAACCTTGTGTGTAAGAACCCTGGCGTCTTGTCTGTAAGCCTCAAGAATCAAATAGTCCCAACCTATGAATTGGTGTACAGATTCTTGCAATCTGACAAGGAAATTTTGGCATTGTTGAATTACAATGCAAATTTACTTTGTGATCGTACTGTGCATAATAACATCACAATGCTGATTGAGAATGGAGTGTCCCACTCAAACATTAAAAGATTGCTTAGGGAACATGCTCGAGCATTCAAGACACGAGACATGCTCAAGTTGGTGAAGGAATTAAAGGATTTGGGGTTTAATCATTCAAAAACAACTTTTGGAGTAGCATTGATAGCAAAATCATCAACAACCAAAACCTTGTGGAATGAGAAAGTTGATGCCTTTAAGAAATGGGGTTGGTCGGATGAAGATGTTCTTCAAGCATTTAGAAAGCAGCCTCACTGTATGATAACATCCATAGATAAAATTAATTTAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCGTTGCCAAAACACCTGATATTTTGTCATTAAGTTTGGAAAAAAGGATCATTCCACGAGCCACTGTTGTGCAATTTCTTCTCAATAATGGTTTGCGAAGTAAGAATTCAAGCTTAATTTATCCATTCTTAAGGTCTGAGAAGTTGTTTCTTGATATGTTTATAAAACGTTTTAAGAATGAGTCTTCTTATCTTATAAAGTTGTATCAGGAAAAACTCAAGCTTGCATACACCAGGGACAAGACATGA
Protein sequence

>MS.gene004584.t1

MFKFNNTWRHRTLIYLKGLTYPQKSQSLNPNPLFQHYPFLFSLHFCTNASDSTSFAVSYLIHNFGLDPQSASKLCSSYKLRFKTHQNPDSVLNFFRNHGFSNSQLRRIIPKAPWLLPCDPSKRVLPKFQFFLSKGASTSDIVNLVCKNPGVLSVSLKNQIVPTYELVYRFLQSDKEILALLNYNANLLCDRTVHNNITMLIENGVSHSNIKRLLREHARAFKTRDMLKLVKELKDLGFNHSKTTFGVALIAKSSTTKTLWNEKVDAFKKWGWSDEDVLQAFRKQPHCMITSIDKINLVMSFWVNQLGWDAMAVAKTPDILSLSLEKRIIPRATVVQFLLNNGLRSKNSSLIYPFLRSEKLFLDMFIKRFKNESSYLIKLYQEKLKLAYTRDKT