AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004592

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004592.t1 MTR_2g437240 78.606 416 83 2 1 416 1 410 0.0 655
MS.gene004592.t1 MTR_2g437200 73.515 404 101 2 1 404 1 398 0.0 597
MS.gene004592.t1 MTR_2g438020 73.890 383 99 1 37 419 70 451 0.0 568
MS.gene004592.t1 MTR_2g437150 65.250 400 100 3 25 420 42 406 0.0 516
MS.gene004592.t1 MTR_2g437170 64.851 404 102 4 19 417 35 403 0.0 512
MS.gene004592.t1 MTR_2g437160 62.060 398 113 3 25 420 50 411 8.87e-173 489
MS.gene004592.t1 MTR_2g437120 63.198 394 107 3 27 418 40 397 2.64e-170 483
MS.gene004592.t1 MTR_2g437100 65.979 388 89 4 25 404 43 395 1.81e-169 481
MS.gene004592.t1 MTR_2g437960 63.351 382 103 2 38 417 53 399 1.56e-167 476
MS.gene004592.t1 MTR_2g437960 63.351 382 103 2 38 417 53 399 1.98e-167 476
MS.gene004592.t1 MTR_2g438010 59.569 418 123 3 13 419 36 418 9.22e-166 472
MS.gene004592.t1 MTR_2g437180 61.558 398 108 4 25 420 42 396 3.26e-165 469
MS.gene004592.t1 MTR_2g076320 59.905 419 122 3 12 419 15 398 3.43e-163 465
MS.gene004592.t1 MTR_2g437990 59.189 419 124 4 12 419 15 397 2.58e-161 461
MS.gene004592.t1 MTR_2g437130 55.000 400 134 4 21 419 32 386 1.67e-143 414
MS.gene004592.t1 MTR_2g437260 52.439 410 153 5 1 408 1 370 1.81e-143 414
MS.gene004592.t1 MTR_2g437940 56.927 397 100 4 25 419 42 369 1.51e-141 409
MS.gene004592.t1 MTR_2g437080 49.626 401 158 4 18 416 24 382 1.36e-130 381
MS.gene004592.t1 MTR_2g436440 49.626 401 158 4 18 416 30 388 3.93e-130 380
MS.gene004592.t1 MTR_2g437060 50.000 394 157 3 21 412 38 393 2.42e-127 373
MS.gene004592.t1 MTR_2g437020 50.882 397 148 6 20 412 25 378 7.29e-127 372
MS.gene004592.t1 MTR_2g437040 47.870 399 164 4 20 416 26 382 1.64e-121 358
MS.gene004592.t1 MTR_4g119550 47.244 381 162 3 33 412 46 388 3.66e-117 347
MS.gene004592.t1 MTR_2g437030 47.103 397 166 4 22 416 114 468 5.21e-116 347
MS.gene004592.t1 MTR_2g436400 45.209 407 176 6 7 408 17 381 5.28e-110 328
MS.gene004592.t1 MTR_2g436460 45.600 375 165 5 32 405 41 377 4.59e-100 303
MS.gene004592.t1 MTR_2g437160 73.936 188 49 0 233 420 133 320 4.80e-97 293
MS.gene004592.t1 MTR_2g437160 32.683 205 86 3 25 180 50 251 2.04e-23 100
MS.gene004592.t1 MTR_4g120380 43.514 370 171 2 33 402 39 370 1.89e-95 291
MS.gene004592.t1 MTR_4g119580 42.971 377 176 3 26 402 33 370 3.91e-95 290
MS.gene004592.t1 MTR_4g120380 44.000 350 158 2 33 382 39 350 7.91e-90 278
MS.gene004592.t1 MTR_2g019810 41.481 405 180 8 7 404 5 359 3.57e-82 257
MS.gene004592.t1 MTR_2g019840 39.950 398 197 6 16 411 21 378 4.90e-82 257
MS.gene004592.t1 MTR_2g437090 55.556 171 76 0 247 417 1 171 4.21e-65 206
MS.gene004592.t1 MTR_3g085240 46.371 248 72 7 1 243 21 212 1.20e-51 174
MS.gene004592.t1 MTR_3g085240 58.696 92 21 1 304 395 174 248 5.11e-25 103
MS.gene004592.t1 MTR_4g119570 38.889 252 118 2 151 402 14 229 2.13e-50 170
MS.gene004592.t1 MTR_3g450500 27.708 397 242 5 15 402 10 370 2.97e-47 166
MS.gene004592.t1 MTR_4g007490 28.997 369 223 4 37 402 36 368 5.17e-46 163
MS.gene004592.t1 MTR_2g436370 41.573 178 92 4 9 179 18 190 4.39e-32 121
MS.gene004592.t1 MTR_8g080990 28.012 332 200 4 69 398 74 368 1.11e-29 119
MS.gene004592.t1 MTR_8g081000 25.146 342 217 3 63 402 81 385 1.74e-28 115
MS.gene004592.t1 MTR_8g081000 25.146 342 217 3 63 402 81 385 2.14e-28 115
MS.gene004592.t1 MTR_8g081000 25.146 342 217 3 63 402 81 385 3.55e-28 115
MS.gene004592.t1 MTR_4g049400 41.732 127 67 2 25 144 37 163 5.35e-25 102
MS.gene004592.t1 MTR_2g436380 42.424 99 54 2 247 345 1 96 5.40e-16 78.2
MS.gene004592.t1 MTR_8g012210 20.065 309 238 5 118 418 63 370 1.84e-12 68.6
MS.gene004592.t1 MTR_4g119615 53.704 54 24 1 341 394 22 74 2.05e-11 60.5
MS.gene004592.t1 MTR_2g019830 56.452 62 26 1 344 405 140 200 8.05e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004592.t1 AT5G07900 30.750 400 236 4 8 401 35 399 5.99e-56 190
MS.gene004592.t1 AT1G21150 30.548 383 218 4 20 399 11 348 3.82e-48 168
MS.gene004592.t1 AT1G21150 30.685 365 215 3 38 399 55 384 1.66e-47 167
MS.gene004592.t1 AT1G21150 30.685 365 215 3 38 399 119 448 5.68e-47 167
MS.gene004592.t1 AT1G61980 27.179 390 205 7 37 387 50 399 1.12e-35 136
MS.gene004592.t1 AT1G61980 27.179 390 205 7 37 387 50 399 1.12e-35 136
MS.gene004592.t1 AT1G61980 27.179 390 205 7 37 387 50 399 1.12e-35 136
MS.gene004592.t1 AT1G61990 26.730 419 221 8 23 401 36 408 3.43e-34 132
MS.gene004592.t1 AT1G61990 26.730 419 221 8 23 401 36 408 3.43e-34 132
MS.gene004592.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene004592.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene004592.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene004592.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene004592.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene004592.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene004592.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene004592.t1 AT1G62120 25.714 385 208 5 22 364 33 381 7.28e-29 117
MS.gene004592.t1 AT3G46950 27.687 307 177 6 13 316 24 288 2.62e-28 116
MS.gene004592.t1 AT5G64950 24.810 395 241 8 23 402 23 376 4.94e-25 106
MS.gene004592.t1 AT1G62150 23.367 398 259 10 37 393 56 448 5.00e-24 103
MS.gene004592.t1 AT1G61960 28.525 305 175 6 17 316 27 293 1.12e-23 103
MS.gene004592.t1 AT5G23930 26.359 368 233 10 30 364 46 408 4.34e-22 98.6
MS.gene004592.t1 AT1G62085 25.693 397 234 11 39 420 55 405 5.33e-22 98.2
MS.gene004592.t1 AT1G62085 25.693 397 234 11 39 420 55 405 5.33e-22 98.2
MS.gene004592.t1 AT1G62110 27.500 280 164 6 39 316 52 294 1.03e-19 91.3
MS.gene004592.t1 AT1G56380 23.989 371 240 12 40 387 17 368 3.44e-18 86.3
MS.gene004592.t1 AT1G56380 23.989 371 240 12 40 387 28 379 3.67e-18 86.3
MS.gene004592.t1 AT1G79220 21.067 356 233 7 73 418 82 399 3.22e-12 68.2

Find 72 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTGATTTGACGAATTT+AGG 0.087763 2.2:+52095449 None:intergenic
CATCCTTCATTGACTACTTT+TGG 0.124797 2.2:-52095065 MS.gene004592:CDS
GTCAATTTGATGTCTGATTT+TGG 0.159011 2.2:-52095191 MS.gene004592:CDS
CGAACAAGGCCATCTATATT+TGG 0.170334 2.2:-52095137 MS.gene004592:CDS
CAAGTTTGTTTGCTCCTTTA+AGG 0.209354 2.2:-52094691 MS.gene004592:CDS
ATAATCAGATTCCTCCTTAA+AGG 0.220620 2.2:+52094677 None:intergenic
TAGAGGAAGTTAAGGGTTTA+GGG 0.232380 2.2:-52095091 MS.gene004592:CDS
TTAGAGGAAGTTAAGGGTTT+AGG 0.237816 2.2:-52095092 MS.gene004592:CDS
AGACCAGCCCCATTTGTTAA+AGG 0.263441 2.2:+52094983 None:intergenic
AACTTGATTTGACGAATTTA+GGG 0.271121 2.2:+52095450 None:intergenic
TGGCATGCCATTCACAAATT+AGG 0.295156 2.2:-52094601 None:intergenic
ATGCCATTCTTTGTTTAATT+CGG 0.306732 2.2:-52095262 MS.gene004592:CDS
AACTTCTCAGAATATCTAAA+TGG 0.329338 2.2:+52094723 None:intergenic
AAGCTAGATTTCAAAATTGT+AGG 0.330980 2.2:+52095344 None:intergenic
ACAAAACATCTGTGGTAGTT+TGG 0.331948 2.2:+52094839 None:intergenic
TCCTACCTCATTCACAAATT+CGG 0.344796 2.2:-52095716 MS.gene004592:CDS
TGCGGTTGAGGTTGTTGATA+TGG 0.356705 2.2:+52095808 None:intergenic
ATTGAAACCAACATCAAATT+AGG 0.361121 2.2:+52094927 None:intergenic
TAAGTCATTAGAGGAAGTTA+AGG 0.381295 2.2:-52095099 MS.gene004592:CDS
GACATTCTTCGTATGGTTGT+AGG 0.384943 2.2:-52095476 MS.gene004592:CDS
AAGAAGTTGAGAACTGAATC+TGG 0.385783 2.2:+52095632 None:intergenic
AGAATATTGGAGTGCGGTTG+AGG 0.397179 2.2:+52095796 None:intergenic
TCCTTTGAACCAAATATAGA+TGG 0.398078 2.2:+52095128 None:intergenic
ACAAAGAATGGCATCCTTGT+TGG 0.401490 2.2:+52095271 None:intergenic
AAGTTGACACCTTTAACAAA+TGG 0.405620 2.2:-52094992 MS.gene004592:CDS
ATCAGAGGAAGAAGCACCTT+TGG 0.406640 2.2:+52095391 None:intergenic
TGAGGAATCTTAGAGAATAT+TGG 0.425832 2.2:+52095783 None:intergenic
GGATTTGATTAAGTCATTAG+AGG 0.426999 2.2:-52095108 MS.gene004592:CDS
AAGTCATTAGAGGAAGTTAA+GGG 0.427528 2.2:-52095098 MS.gene004592:CDS
GCCATCTATATTTGGTTCAA+AGG 0.427799 2.2:-52095129 MS.gene004592:CDS
GAAATTACCTAATTTGTGAA+TGG 0.433382 2.2:+52094594 None:intergenic
AGTTGACACCTTTAACAAAT+GGG 0.435374 2.2:-52094991 MS.gene004592:CDS
CTCAACTTCTTCAAAAGTCA+TGG 0.435992 2.2:-52095620 MS.gene004592:CDS
TCAATTGGGTTGGAATTCCT+TGG 0.440659 2.2:-52094868 MS.gene004592:CDS
GTCAGAGGTAGAAGCACCTT+TGG 0.453352 2.2:+52095496 None:intergenic
AGCTAGATTTCAAAATTGTA+GGG 0.454084 2.2:+52095345 None:intergenic
TACCTCTGACATTCTTCGTA+TGG 0.462578 2.2:-52095483 MS.gene004592:CDS
TTTCATGCAATGCCCACAAA+AGG 0.467899 2.2:-52095547 MS.gene004592:CDS
ATTATCCACAGTAAGGTGAT+AGG 0.484709 2.2:+52095214 None:intergenic
ATGCCGAATTAAACAAAGAA+TGG 0.493192 2.2:+52095259 None:intergenic
TCTAAATGGTGTAACTAAGC+TGG 0.495852 2.2:+52094737 None:intergenic
TTTGGGTCAATCAATTGGGT+TGG 0.506924 2.2:-52094878 MS.gene004592:CDS
GTTGCAGCCTAATTTGATGT+TGG 0.507199 2.2:-52094934 MS.gene004592:CDS
ACACCTTTAACAAATGGGGC+TGG 0.542117 2.2:-52094986 MS.gene004592:CDS
AATTGCATAACTGAGGCCCT+TGG 0.542717 2.2:+52094792 None:intergenic
ACCCCAAAAGTAGTCAATGA+AGG 0.557831 2.2:+52095062 None:intergenic
TTGCTAGATTGCTTCGAACA+AGG 0.560619 2.2:-52095151 MS.gene004592:CDS
AATTGACATTATCCACAGTA+AGG 0.560808 2.2:+52095207 None:intergenic
TGGAGAAAAGGATCATTCCA+AGG 0.570897 2.2:-52094809 MS.gene004592:CDS
TGGTAGTTTGGCAAGTGCCA+AGG 0.586106 2.2:+52094851 None:intergenic
TGAGGTTGTTGATATGGCAA+AGG 0.590012 2.2:+52095814 None:intergenic
GTTTGTTTGCTCCTTTAAGG+AGG 0.603996 2.2:-52094688 MS.gene004592:CDS
AGAAACCGAATTTGTGAATG+AGG 0.604803 2.2:+52095711 None:intergenic
ACCGAATTTGTGAATGAGGT+AGG 0.607992 2.2:+52095715 None:intergenic
AATGAAACTTGCATACACAA+TGG 0.609213 2.2:-52094631 MS.gene004592:CDS
TGAATTTGAATCTGAAGCGA+TGG 0.615539 2.2:+52095754 None:intergenic
TAACAATGCAACAATATCAG+AGG 0.616711 2.2:+52095376 None:intergenic
TGAATCTGAAGCGATGGTAG+TGG 0.621211 2.2:+52095760 None:intergenic
CAAAAGAAATTGCATAACTG+AGG 0.634260 2.2:+52094785 None:intergenic
GTTGACACCTTTAACAAATG+GGG 0.635802 2.2:-52094990 MS.gene004592:CDS
GCATTGCATGAAAGAAGCCA+TGG 0.636869 2.2:+52095557 None:intergenic
AAACTAAAACAAAACATCTG+TGG 0.637333 2.2:+52094831 None:intergenic
GGAGAAAAGGATCATTCCAA+GGG 0.639024 2.2:-52094808 MS.gene004592:CDS
TTGATATGGCAAAGGCCTTG+CGG 0.640767 2.2:+52095822 None:intergenic
GCATACACAATGGAAAACAA+TGG 0.644592 2.2:-52094621 MS.gene004592:CDS
CTGAAGCGATGGTAGTGGTG+AGG 0.647119 2.2:+52095765 None:intergenic
TCTTAGAGAATATTGGAGTG+CGG 0.670463 2.2:+52095790 None:intergenic
AACCATACGAAGAATGTCAG+AGG 0.672395 2.2:+52095481 None:intergenic
GAATACCTATCACCTTACTG+TGG 0.678196 2.2:-52095219 MS.gene004592:CDS
GCATCATCAAAAGAGAACCA+TGG 0.694296 2.2:-52095574 MS.gene004592:CDS
TAGGTTTCTGAAAACCAACA+AGG 0.701717 2.2:-52095285 MS.gene004592:CDS
TTATCTATTAAAGCTATACG+AGG 0.742253 2.2:-52094658 MS.gene004592:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTAAAAATTCAAAAAGA+GGG + chr2.2:52095141-52095160 None:intergenic 15.0%
!! TGATTAAAAATTGAAACTTT+GGG + chr2.2:52094928-52094947 None:intergenic 15.0%
!! AATGTATGAGTAAAAAACTT+TGG - chr2.2:52095422-52095441 MS.gene004592:CDS 20.0%
!! ATGTATGAGTAAAAAACTTT+GGG - chr2.2:52095423-52095442 MS.gene004592:CDS 20.0%
!! CTACTTAAAAATTCAAAAAG+AGG + chr2.2:52095142-52095161 None:intergenic 20.0%
!! GTGATTAAAAATTGAAACTT+TGG + chr2.2:52094929-52094948 None:intergenic 20.0%
!!! CTCTTTTTGAATTTTTAAGT+AGG - chr2.2:52095140-52095159 MS.gene004592:CDS 20.0%
!!! TTTCAATTTTTAATCACCAA+AGG - chr2.2:52094932-52094951 MS.gene004592:CDS 20.0%
!!! TTTCATTTTTTTCTATCCAA+AGG - chr2.2:52095037-52095056 MS.gene004592:CDS 20.0%
! AAACTAAAACAAAACATCTG+TGG + chr2.2:52095616-52095635 None:intergenic 25.0%
! AACTTCTCAGAATATCTAAA+TGG + chr2.2:52095724-52095743 None:intergenic 25.0%
! AAGCTAGATTTCAAAATTGT+AGG + chr2.2:52095103-52095122 None:intergenic 25.0%
! AGCTAGATTTCAAAATTGTA+GGG + chr2.2:52095102-52095121 None:intergenic 25.0%
! ATTGAAACCAACATCAAATT+AGG + chr2.2:52095520-52095539 None:intergenic 25.0%
! TTATCTATTAAAGCTATACG+AGG - chr2.2:52095786-52095805 MS.gene004592:CDS 25.0%
!! AAACTTGATTTGACGAATTT+AGG + chr2.2:52094998-52095017 None:intergenic 25.0%
!! AACTTGATTTGACGAATTTA+GGG + chr2.2:52094997-52095016 None:intergenic 25.0%
!! ATGCAATTTCTTTTGATGAA+AGG - chr2.2:52095667-52095686 MS.gene004592:CDS 25.0%
!! ATGCCATTCTTTGTTTAATT+CGG - chr2.2:52095182-52095201 MS.gene004592:CDS 25.0%
!! TGATTCGATAATGAGTTTTT+GGG - chr2.2:52095549-52095568 MS.gene004592:CDS 25.0%
!!! ACAGATGTTTTGTTTTAGTT+TGG - chr2.2:52095615-52095634 MS.gene004592:CDS 25.0%
!!! TACAATTTTGAAATCTAGCT+TGG - chr2.2:52095102-52095121 MS.gene004592:CDS 25.0%
!!! TTGATTCGATAATGAGTTTT+TGG - chr2.2:52095548-52095567 MS.gene004592:CDS 25.0%
!!! TTTGTTTTAGTTTGGAGAAA+AGG - chr2.2:52095623-52095642 MS.gene004592:CDS 25.0%
!!! TTTTTTACTCATACATTGCT+TGG + chr2.2:52095419-52095438 None:intergenic 25.0%
AAGTCATTAGAGGAAGTTAA+GGG - chr2.2:52095346-52095365 MS.gene004592:CDS 30.0%
AAGTTGACACCTTTAACAAA+TGG - chr2.2:52095452-52095471 MS.gene004592:CDS 30.0%
AATGAAACTTGCATACACAA+TGG - chr2.2:52095813-52095832 MS.gene004592:CDS 30.0%
AATTGACATTATCCACAGTA+AGG + chr2.2:52095240-52095259 None:intergenic 30.0%
AGTTGACACCTTTAACAAAT+GGG - chr2.2:52095453-52095472 MS.gene004592:CDS 30.0%
ATAATCAGATTCCTCCTTAA+AGG + chr2.2:52095770-52095789 None:intergenic 30.0%
ATGCCGAATTAAACAAAGAA+TGG + chr2.2:52095188-52095207 None:intergenic 30.0%
CAAAAGAAATTGCATAACTG+AGG + chr2.2:52095662-52095681 None:intergenic 30.0%
GGATTTGATTAAGTCATTAG+AGG - chr2.2:52095336-52095355 MS.gene004592:CDS 30.0%
TAACAATGCAACAATATCAG+AGG + chr2.2:52095071-52095090 None:intergenic 30.0%
TAAGTCATTAGAGGAAGTTA+AGG - chr2.2:52095345-52095364 MS.gene004592:CDS 30.0%
TCCTTTGAACCAAATATAGA+TGG + chr2.2:52095319-52095338 None:intergenic 30.0%
TGAGGAATCTTAGAGAATAT+TGG + chr2.2:52094664-52094683 None:intergenic 30.0%
! GTCAATTTGATGTCTGATTT+TGG - chr2.2:52095253-52095272 MS.gene004592:CDS 30.0%
!! ATCCTTCATTGACTACTTTT+GGG - chr2.2:52095380-52095399 MS.gene004592:CDS 30.0%
!!! AGTTTTTGGGTCAATCAATT+GGG - chr2.2:52095562-52095581 MS.gene004592:CDS 30.0%
!!! GGTTTTGAAACGAAGATTTT+TGG + chr2.2:52094783-52094802 None:intergenic 30.0%
AAGAAGTTGAGAACTGAATC+TGG + chr2.2:52094815-52094834 None:intergenic 35.0%
ACAAAACATCTGTGGTAGTT+TGG + chr2.2:52095608-52095627 None:intergenic 35.0%
AGAAACCGAATTTGTGAATG+AGG + chr2.2:52094736-52094755 None:intergenic 35.0%
ATTATCCACAGTAAGGTGAT+AGG + chr2.2:52095233-52095252 None:intergenic 35.0%
CTCAACTTCTTCAAAAGTCA+TGG - chr2.2:52094824-52094843 MS.gene004592:CDS 35.0%
GCATACACAATGGAAAACAA+TGG - chr2.2:52095823-52095842 MS.gene004592:CDS 35.0%
GTTGACACCTTTAACAAATG+GGG - chr2.2:52095454-52095473 MS.gene004592:CDS 35.0%
TAGAGGAAGTTAAGGGTTTA+GGG - chr2.2:52095353-52095372 MS.gene004592:CDS 35.0%
TCCTACCTCATTCACAAATT+CGG - chr2.2:52094728-52094747 MS.gene004592:CDS 35.0%
TCTAAATGGTGTAACTAAGC+TGG + chr2.2:52095710-52095729 None:intergenic 35.0%
TCTTAGAGAATATTGGAGTG+CGG + chr2.2:52094657-52094676 None:intergenic 35.0%
TTAGAGGAAGTTAAGGGTTT+AGG - chr2.2:52095352-52095371 MS.gene004592:CDS 35.0%
! CAAGTTTGTTTGCTCCTTTA+AGG - chr2.2:52095753-52095772 MS.gene004592:CDS 35.0%
! GCCATCTATATTTGGTTCAA+AGG - chr2.2:52095315-52095334 MS.gene004592:CDS 35.0%
! TAGGTTTCTGAAAACCAACA+AGG - chr2.2:52095159-52095178 MS.gene004592:CDS 35.0%
! TGAATTTGAATCTGAAGCGA+TGG + chr2.2:52094693-52094712 None:intergenic 35.0%
!! CATCCTTCATTGACTACTTT+TGG - chr2.2:52095379-52095398 MS.gene004592:CDS 35.0%
!! GAAACTTTTTCTATTCCGCA+AGG - chr2.2:52094607-52094626 MS.gene004592:CDS 35.0%
!! TCCTTCATTGACTACTTTTG+GGG - chr2.2:52095381-52095400 MS.gene004592:CDS 35.0%
!!! AACTGAATCTGGTTTTTGTG+AGG + chr2.2:52094804-52094823 None:intergenic 35.0%
!!! GAGTTTTTGGGTCAATCAAT+TGG - chr2.2:52095561-52095580 MS.gene004592:CDS 35.0%
AACCATACGAAGAATGTCAG+AGG + chr2.2:52094966-52094985 None:intergenic 40.0%
ACAAAGAATGGCATCCTTGT+TGG + chr2.2:52095176-52095195 None:intergenic 40.0%
ACCCCAAAAGTAGTCAATGA+AGG + chr2.2:52095385-52095404 None:intergenic 40.0%
ACCGAATTTGTGAATGAGGT+AGG + chr2.2:52094732-52094751 None:intergenic 40.0%
CGAACAAGGCCATCTATATT+TGG - chr2.2:52095307-52095326 MS.gene004592:CDS 40.0%
GAATACCTATCACCTTACTG+TGG - chr2.2:52095225-52095244 MS.gene004592:CDS 40.0%
GACATTCTTCGTATGGTTGT+AGG - chr2.2:52094968-52094987 MS.gene004592:CDS 40.0%
GCATCATCAAAAGAGAACCA+TGG - chr2.2:52094870-52094889 MS.gene004592:CDS 40.0%
GGAGAAAAGGATCATTCCAA+GGG - chr2.2:52095636-52095655 MS.gene004592:CDS 40.0%
GTTGCAGCCTAATTTGATGT+TGG - chr2.2:52095510-52095529 MS.gene004592:CDS 40.0%
TACCTCTGACATTCTTCGTA+TGG - chr2.2:52094961-52094980 MS.gene004592:CDS 40.0%
TGAGGTTGTTGATATGGCAA+AGG + chr2.2:52094633-52094652 None:intergenic 40.0%
TGGAGAAAAGGATCATTCCA+AGG - chr2.2:52095635-52095654 MS.gene004592:CDS 40.0%
TTGCTAGATTGCTTCGAACA+AGG - chr2.2:52095293-52095312 MS.gene004592:CDS 40.0%
TTTCATGCAATGCCCACAAA+AGG - chr2.2:52094897-52094916 MS.gene004592:CDS 40.0%
TTTGGGTCAATCAATTGGGT+TGG - chr2.2:52095566-52095585 MS.gene004592:CDS 40.0%
! GTTTGTTTGCTCCTTTAAGG+AGG - chr2.2:52095756-52095775 MS.gene004592:CDS 40.0%
!! ACTTTGGGAGAATCCTTTTG+TGG + chr2.2:52094913-52094932 None:intergenic 40.0%
!! CTTTGGGAGAATCCTTTTGT+GGG + chr2.2:52094912-52094931 None:intergenic 40.0%
!! TCAATTGGGTTGGAATTCCT+TGG - chr2.2:52095576-52095595 MS.gene004592:CDS 40.0%
AATTGCATAACTGAGGCCCT+TGG + chr2.2:52095655-52095674 None:intergenic 45.0%
ACACCTTTAACAAATGGGGC+TGG - chr2.2:52095458-52095477 MS.gene004592:CDS 45.0%
AGAATATTGGAGTGCGGTTG+AGG + chr2.2:52094651-52094670 None:intergenic 45.0%
GCATTGCATGAAAGAAGCCA+TGG + chr2.2:52094890-52094909 None:intergenic 45.0%
TGCGGTTGAGGTTGTTGATA+TGG + chr2.2:52094639-52094658 None:intergenic 45.0%
TTGATATGGCAAAGGCCTTG+CGG + chr2.2:52094625-52094644 None:intergenic 45.0%
! AGACCAGCCCCATTTGTTAA+AGG + chr2.2:52095464-52095483 None:intergenic 45.0%
! TGAATCTGAAGCGATGGTAG+TGG + chr2.2:52094687-52094706 None:intergenic 45.0%
!! ATCAGAGGAAGAAGCACCTT+TGG + chr2.2:52095056-52095075 None:intergenic 45.0%
!!! GACTACTTTTGGGGTTGCTT+TGG - chr2.2:52095390-52095409 MS.gene004592:CDS 45.0%
TGGTAGTTTGGCAAGTGCCA+AGG + chr2.2:52095596-52095615 None:intergenic 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG + chr2.2:52094951-52094970 None:intergenic 50.0%
!! CTGAAGCGATGGTAGTGGTG+AGG + chr2.2:52094682-52094701 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 52094602 52095864 52094602 ID=MS.gene004592
chr2.2 mRNA 52094602 52095864 52094602 ID=MS.gene004592.t1;Parent=MS.gene004592
chr2.2 exon 52094602 52095864 52094602 ID=MS.gene004592.t1.exon1;Parent=MS.gene004592.t1
chr2.2 CDS 52094602 52095864 52094602 ID=cds.MS.gene004592.t1;Parent=MS.gene004592.t1
Gene Sequence

>MS.gene004592

ATGTTGAAACTTTTTCTATTCCGCAAGGCCTTTGCCATATCAACAACCTCAACCGCACTCCAATATTCTCTAAGATTCCTCACCACTACCATCGCTTCAGATTCAAATTCAAATTCATTCGCTGTATCCTACCTCATTCACAAATTCGGTTTCTCACATCAATTCGCACTCAAAGCTTCCAAAAATCTTCGTTTCAAAACCTCACAAAAACCAGATTCAGTTCTCAACTTCTTCAAAAGTCATGGTTTCACTGACTCAGATATACACAGCATCATCAAAAGAGAACCATGGCTTCTTTCATGCAATGCCCACAAAAGGATTCTCCCAAAGTTTCAATTTTTAATCACCAAAGGTGCTTCTACCTCTGACATTCTTCGTATGGTTGTAGGTAACCCTAAATTCGTCAAATCAAGTTTGAAGAATCATGAAACTATGTTTCATTTTTTTCTATCCAAAGGTGCTTCTTCCTCTGATATTGTTGCATTGTTAACTGCTAACCCTACAATTTTGAAATCTAGCTTGGAGAGACGAATAATCCCTCTTTTTGAATTTTTAAGTAGGTTTCTGAAAACCAACAAGGATGCCATTCTTTGTTTAATTCGGCATTCGACTGCATTTTCTATGAATACCTATCACCTTACTGTGGATAATGTCAATTTGATGTCTGATTTTGGAGTTTCTGATTCTACAATTGCTAGATTGCTTCGAACAAGGCCATCTATATTTGGTTCAAAGGATTTGATTAAGTCATTAGAGGAAGTTAAGGGTTTAGGGTTTCATCCTTCATTGACTACTTTTGGGGTTGCTTTGGTAGCCAAGCAATGTATGAGTAAAAAACTTTGGGATGAGAAAGTTGACACCTTTAACAAATGGGGCTGGTCTGATGAAGCTGTTATTCAAACATTTCAGTTGCAGCCTAATTTGATGTTGGTTTCAATTGATAAGATTGATTCGATAATGAGTTTTTGGGTCAATCAATTGGGTTGGAATTCCTTGGCACTTGCCAAACTACCACAGATGTTTTGTTTTAGTTTGGAGAAAAGGATCATTCCAAGGGCCTCAGTTATGCAATTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCCAGCTTAGTTACACCATTTAGATATTCTGAGAAGTTGTTCTTGAACAAGTTTGTTTGCTCCTTTAAGGAGGAATCTGATTATCTATTAAAGCTATACGAGGAAAAAATGAAACTTGCATACACAATGGAAAACAATGGCATGCCATTCACAAATTAG
Protein sequence

>MS.gene004592.t1

MLKLFLFRKAFAISTTSTALQYSLRFLTTTIASDSNSNSFAVSYLIHKFGFSHQFALKASKNLRFKTSQKPDSVLNFFKSHGFTDSDIHSIIKREPWLLSCNAHKRILPKFQFLITKGASTSDILRMVVGNPKFVKSSLKNHETMFHFFLSKGASSSDIVALLTANPTILKSSLERRIIPLFEFLSRFLKTNKDAILCLIRHSTAFSMNTYHLTVDNVNLMSDFGVSDSTIARLLRTRPSIFGSKDLIKSLEEVKGLGFHPSLTTFGVALVAKQCMSKKLWDEKVDTFNKWGWSDEAVIQTFQLQPNLMLVSIDKIDSIMSFWVNQLGWNSLALAKLPQMFCFSLEKRIIPRASVMQFLLMKGLRKKNASLVTPFRYSEKLFLNKFVCSFKEESDYLLKLYEEKMKLAYTMENNGMPFTN