AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004636

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 121 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTATGTGGAGGCGGATTT+TGG 0.148513 2.2:-51575238 None:intergenic
AGATTTGTTGCACGAGTGTT+TGG 0.301888 2.2:+51575585 MS.gene004636:CDS
TTCCTCCAGCGGCATTTCTC+AGG 0.312507 2.2:+51575694 MS.gene004636:CDS
CCTGATTGCTGCCGAGATAC+TGG 0.331416 2.2:+51574995 MS.gene004636:CDS
TTCGGGATGAGGAGGAGAAT+TGG 0.336432 2.2:-51575174 None:intergenic
ACTCGTGCAACAAATCTTCT+TGG 0.342234 2.2:-51575579 None:intergenic
GGTGGGAATGTGGCTAATAA+TGG 0.350488 2.2:+51575389 MS.gene004636:CDS
CTATAGGCTAAGGAGTTTGC+TGG 0.354712 2.2:+51574836 MS.gene004636:CDS
ACTCCTTAGCCTATAGAAAA+AGG 0.359699 2.2:-51574829 None:intergenic
TAAGACACACTTACCTTGTC+TGG 0.362065 2.2:-51574010 None:intergenic
TTCAACTCCTGTGGCAGAAC+CGG 0.369949 2.2:+51575127 MS.gene004636:CDS
TCCCTAGCATACATAACATA+TGG 0.379144 2.2:-51575795 None:intergenic
GACACAAGAGGTTCGAGGTT+TGG 0.386987 2.2:+51574777 MS.gene004636:CDS
TCATGGCCGCGCTTACATTC+AGG 0.392786 2.2:+51573966 None:intergenic
TGGCAGTTGGGAAAAGTGTT+AGG 0.395161 2.2:+51575751 MS.gene004636:CDS
GGCATTGTGTGGTTGTTGAT+TGG 0.403874 2.2:-51575207 None:intergenic
TGATTGGCATCATGAACTTC+GGG 0.411728 2.2:-51575191 None:intergenic
CGGAGAACCGGCTGGCAGTT+GGG 0.421662 2.2:+51575739 MS.gene004636:CDS
TGATGCTGCTGCTGTTGCCA+TGG 0.428743 2.2:+51575340 MS.gene004636:CDS
TCCTCCAGCGGCATTTCTCA+GGG 0.443998 2.2:+51575695 MS.gene004636:CDS
CGGACAGGGGCTGTTCTACA+TGG 0.447788 2.2:+51574905 MS.gene004636:CDS
TCGGGATGAGGAGGAGAATT+GGG 0.451241 2.2:-51575173 None:intergenic
GACGATGATTCCATCGATCT+TGG 0.451452 2.2:+51575074 MS.gene004636:CDS
GATTTGTTGCACGAGTGTTT+GGG 0.452697 2.2:+51575586 MS.gene004636:CDS
AGAGATTTGTTACGGAGAAC+CGG 0.452877 2.2:+51575727 MS.gene004636:CDS
GTGGGAATGTGGCTAATAAT+GGG 0.456886 2.2:+51575390 MS.gene004636:CDS
CCAGTATCTCGGCAGCAATC+AGG 0.457630 2.2:-51574995 None:intergenic
GAGCACTTCCACCAGCCGTT+AGG 0.466631 2.2:+51574858 MS.gene004636:CDS
CGAGATACTGGATCCCTTGG+AGG 0.468114 2.2:+51575007 MS.gene004636:CDS
ATCGTCGTCGTTGTCTTCAT+CGG 0.472155 2.2:-51575057 None:intergenic
ACATCAACATCCGCTGGATC+CGG 0.472816 2.2:-51575146 None:intergenic
TTGATTGGCATCATGAACTT+CGG 0.473733 2.2:-51575192 None:intergenic
CAGCGGCATTTCTCAGGGGC+GGG 0.478818 2.2:+51575700 MS.gene004636:CDS
ATGAACTACGACACAGAGTC+TGG 0.481562 2.2:+51574748 MS.gene004636:CDS
ACAGGAGTTGAAGGAGGTGC+AGG 0.491094 2.2:-51575116 None:intergenic
CATGGTGAAGACTAGCAATC+TGG 0.491937 2.2:+51575448 MS.gene004636:CDS
ACGGAGAACCGGCTGGCAGT+TGG 0.492128 2.2:+51575738 MS.gene004636:CDS
GCTGGATCCGGTTCTGCCAC+AGG 0.493035 2.2:-51575134 None:intergenic
GATGCTGCTGCTGTTGCCAT+GGG 0.497052 2.2:+51575341 MS.gene004636:CDS
TGGGCTTTGACTTTCCGTGC+AGG 0.505024 2.2:+51575646 MS.gene004636:CDS
CGGCATTTCTCAGGGGCGGG+TGG 0.507786 2.2:+51575703 MS.gene004636:CDS
CCCTAGCATACATAACATAT+GGG 0.511261 2.2:-51575794 None:intergenic
GCATCATGAACTTCGGGATG+AGG 0.512150 2.2:-51575185 None:intergenic
GGTTCTGCCACAGGAGTTGA+AGG 0.512252 2.2:-51575125 None:intergenic
TCATAGGAAGTGAGGGGACG+CGG 0.513245 2.2:+51574805 MS.gene004636:CDS
AACGATGAAGATGTGGAAGA+TGG 0.513612 2.2:+51575290 MS.gene004636:CDS
CCAGCGGCATTTCTCAGGGG+CGG 0.516500 2.2:+51575699 MS.gene004636:CDS
GCAAAATCAAAGAAATTATG+TGG 0.521917 2.2:-51575251 None:intergenic
TGCCGAGATACTGGATCCCT+TGG 0.522650 2.2:+51575004 MS.gene004636:CDS
TCATACTTCAACAACCACTT+TGG 0.527976 2.2:+51574723 MS.gene004636:CDS
ACCCATATGTTATGTATGCT+AGG 0.529159 2.2:+51575793 MS.gene004636:CDS
GTATGCTAGGGAAGCTCGGA+TGG 0.537149 2.2:+51575806 MS.gene004636:CDS
AAGTGGTTGTTGAAGTATGA+TGG 0.538484 2.2:-51574720 None:intergenic
AACATCAATGCCAAGATCGA+TGG 0.539611 2.2:-51575084 None:intergenic
GCGTCTAAGTTACAACGGAC+AGG 0.542298 2.2:+51574890 MS.gene004636:CDS
CCGCCCCTGAGAAATGCCGC+TGG 0.546065 2.2:-51575699 None:intergenic
CATCCTACTTACAGATGCCA+TGG 0.546268 2.2:+51575626 MS.gene004636:CDS
ATTTGTTACGGAGAACCGGC+TGG 0.553165 2.2:+51575731 MS.gene004636:CDS
TCAAAGAAATTATGTGGAGG+CGG 0.554584 2.2:-51575245 None:intergenic
ATCTTCATCGTTGATATCCA+TGG 0.554841 2.2:-51575279 None:intergenic
CCCATATGTTATGTATGCTA+GGG 0.556719 2.2:+51575794 MS.gene004636:CDS
ACTCGACATGACCGAGCTAA+CGG 0.557804 2.2:+51574938 MS.gene004636:CDS
CTCCAAGGGATCCAGTATCT+CGG 0.558445 2.2:-51575006 None:intergenic
TAGAAGACACAAGAGGTTCG+AGG 0.561323 2.2:+51574772 MS.gene004636:CDS
TGTGGCTAATAATGGGAGAG+TGG 0.562385 2.2:+51575397 MS.gene004636:CDS
TCCTTCCACTTGATCCTGCA+CGG 0.565208 2.2:-51575660 None:intergenic
TGCACCTCCTTCAACTCCTG+TGG 0.569911 2.2:+51575118 MS.gene004636:CDS
GGTGCAACATCAACATCCGC+TGG 0.570592 2.2:-51575152 None:intergenic
GTATAATTCATACTCCTCCA+AGG 0.570762 2.2:-51575021 None:intergenic
TTATGTATGCTAGGGAAGCT+CGG 0.572454 2.2:+51575802 MS.gene004636:CDS
GGCATCTGTAAGTAGGATGG+TGG 0.575659 2.2:-51575622 None:intergenic
CGTCTAAGTTACAACGGACA+GGG 0.576116 2.2:+51574891 MS.gene004636:CDS
GGTGAAAACATGGGTGCAGG+TGG 0.578164 2.2:+51575371 MS.gene004636:CDS
ACTTTCCGTGCAGGATCAAG+TGG 0.580280 2.2:+51575655 MS.gene004636:CDS
CATGGGTGCAGGTGGGAATG+TGG 0.582106 2.2:+51575379 MS.gene004636:CDS
CCTCTCACGAACCGTTAGCT+CGG 0.586517 2.2:-51574949 None:intergenic
CGGGTGGAAGAGATTTGTTA+CGG 0.590170 2.2:+51575719 MS.gene004636:CDS
GAAATGCCGCTGGAGGAAGA+TGG 0.590786 2.2:-51575689 None:intergenic
CCGAGCTAACGGTTCGTGAG+AGG 0.592918 2.2:+51574949 MS.gene004636:CDS
CCTCCAGCGGCATTTCTCAG+GGG 0.594266 2.2:+51575696 MS.gene004636:CDS
GGAGTTGAAGGAGGTGCAGG+AGG 0.595751 2.2:-51575113 None:intergenic
GGGTAACAATGGTGAAAACA+TGG 0.597431 2.2:+51575361 MS.gene004636:CDS
TGTGGCAGAACCGGATCCAG+CGG 0.598738 2.2:+51575136 MS.gene004636:CDS
CCCCTGAGAAATGCCGCTGG+AGG 0.603022 2.2:-51575696 None:intergenic
TCATGAACTTCGGGATGAGG+AGG 0.603718 2.2:-51575182 None:intergenic
CATGGCATCTGTAAGTAGGA+TGG 0.604155 2.2:-51575625 None:intergenic
GTGCAACAAATCTTCTTGGT+AGG 0.604465 2.2:-51575575 None:intergenic
TCGGCAGCAATCAGGAAAGG+AGG 0.606699 2.2:-51574987 None:intergenic
GCAGTATGAGACCTAACGGC+TGG 0.608111 2.2:-51574869 None:intergenic
AGCCCATGGCATCTGTAAGT+AGG 0.608677 2.2:-51575629 None:intergenic
CACAACAATCGATCCAGACA+AGG 0.610416 2.2:+51573997 MS.gene004636:CDS
ATCTCGGCAGCAATCAGGAA+AGG 0.610893 2.2:-51574990 None:intergenic
ATCCTACTTACAGATGCCAT+GGG 0.611432 2.2:+51575627 MS.gene004636:CDS
TGTCAGACTTGCAGAATACA+TGG 0.612787 2.2:+51575430 MS.gene004636:CDS
GGATATCAACGATGAAGATG+TGG 0.616665 2.2:+51575283 MS.gene004636:CDS
TATAATTCATACTCCTCCAA+GGG 0.616989 2.2:-51575020 None:intergenic
GTATGAGACCTAACGGCTGG+TGG 0.617542 2.2:-51574866 None:intergenic
CGGCAGCAATCAGGAAAGGA+GGG 0.618638 2.2:-51574986 None:intergenic
AATGGTGAAAACATGGGTGC+AGG 0.619307 2.2:+51575368 MS.gene004636:CDS
CAGAGGCCATCTTCCTCCAG+CGG 0.626292 2.2:+51575683 MS.gene004636:CDS
GCATCTGTAAGTAGGATGGT+GGG 0.632206 2.2:-51575621 None:intergenic
GCACGGAAAGTCAAAGCCCA+TGG 0.634938 2.2:-51575643 None:intergenic
TCCGTGCAGGATCAAGTGGA+AGG 0.637315 2.2:+51575659 MS.gene004636:CDS
TGGGCATCATCGGCATTGTG+TGG 0.641687 2.2:-51575218 None:intergenic
GTCGTAGTTCATCACCAAAG+TGG 0.648562 2.2:-51574737 None:intergenic
AGACGCAGTATGAGACCTAA+CGG 0.653534 2.2:-51574873 None:intergenic
GCTGTTGCCATGGGTAACAA+TGG 0.655928 2.2:+51575350 MS.gene004636:CDS
GCAGCATCAAAGAAGTTATG+CGG 0.656305 2.2:-51575326 None:intergenic
GGACAGGGGCTGTTCTACAT+GGG 0.657468 2.2:+51574906 MS.gene004636:CDS
GAACTTCCTGAATGTAAGCG+CGG 0.670661 2.2:-51573972 None:intergenic
GTGAAAACATGGGTGCAGGT+GGG 0.671071 2.2:+51575372 MS.gene004636:CDS
GTTGTCTTCATCGGATGACG+AGG 0.675992 2.2:-51575048 None:intergenic
GCATCAAAGAAGTTATGCGG+AGG 0.686757 2.2:-51575323 None:intergenic
AGGATCAAGTGGAAGGACAG+AGG 0.686964 2.2:+51575666 MS.gene004636:CDS
GTCTGGTTAGAAGACACAAG+AGG 0.687596 2.2:+51574765 MS.gene004636:CDS
ATACTGCGTCTAAGTTACAA+CGG 0.691908 2.2:+51574885 MS.gene004636:CDS
AAATCAAAGAAATTATGTGG+AGG 0.696121 2.2:-51575248 None:intergenic
TCTGCCACAGGAGTTGAAGG+AGG 0.696157 2.2:-51575122 None:intergenic
TCTGTAAGTAGGATGGTGGG+CGG 0.704271 2.2:-51575618 None:intergenic
GTCTAAGTTACAACGGACAG+GGG 0.730281 2.2:+51574892 MS.gene004636:CDS
GGTAACAATGGTGAAAACAT+GGG 0.742892 2.2:+51575362 MS.gene004636:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTTAAAAAAGAACAC+AGG + chr2.2:51574603-51574622 MS.gene004636:intron 15.0%
!!! AAATGATAACAATTTAAAAC+AGG - chr2.2:51575545-51575564 None:intergenic 15.0%
!!! AATTACTGTTATATAGTTTT+TGG + chr2.2:51575504-51575523 MS.gene004636:intron 15.0%
! AAATCAAAGAAATTATGTGG+AGG - chr2.2:51575251-51575270 None:intergenic 25.0%
! AGTACACAAAATCTTAGATA+CGG - chr2.2:51574263-51574282 None:intergenic 25.0%
! GCAAAATCAAAGAAATTATG+TGG - chr2.2:51575254-51575273 None:intergenic 25.0%
!!! CATTTGTTGTTACATGTTTA+AGG + chr2.2:51574150-51574169 MS.gene004636:intron 25.0%
AAATATATTGCAGAGTGACA+AGG - chr2.2:51574346-51574365 None:intergenic 30.0%
AGTACACATTATCGTAGATA+CGG - chr2.2:51574450-51574469 None:intergenic 30.0%
ATTACAGAGTGACAAAAAAC+TGG - chr2.2:51574494-51574513 None:intergenic 30.0%
TATAATTCATACTCCTCCAA+GGG - chr2.2:51575023-51575042 None:intergenic 30.0%
TTACAGAGTGACAAAAAACT+GGG - chr2.2:51574493-51574512 None:intergenic 30.0%
TTGTTACTAAACTTTGAGTG+TGG + chr2.2:51574047-51574066 MS.gene004636:intron 30.0%
AAAATCTACCGGATACCAAA+AGG - chr2.2:51574121-51574140 None:intergenic 35.0%
AAATCTACCGGATACCAAAA+GGG - chr2.2:51574120-51574139 None:intergenic 35.0%
AAGTGGTTGTTGAAGTATGA+TGG - chr2.2:51574723-51574742 None:intergenic 35.0%
ACCCATATGTTATGTATGCT+AGG + chr2.2:51575793-51575812 MS.gene004636:CDS 35.0%
ACTCCTTAGCCTATAGAAAA+AGG - chr2.2:51574832-51574851 None:intergenic 35.0%
ATACTGCGTCTAAGTTACAA+CGG + chr2.2:51574885-51574904 MS.gene004636:CDS 35.0%
ATCTTCATCGTTGATATCCA+TGG - chr2.2:51575282-51575301 None:intergenic 35.0%
ATTACAGAGTGACAACAAAC+AGG - chr2.2:51574307-51574326 None:intergenic 35.0%
CCCATATGTTATGTATGCTA+GGG + chr2.2:51575794-51575813 MS.gene004636:CDS 35.0%
CCCTAGCATACATAACATAT+GGG - chr2.2:51575797-51575816 None:intergenic 35.0%
GGTAACAATGGTGAAAACAT+GGG + chr2.2:51575362-51575381 MS.gene004636:CDS 35.0%
GTATAATTCATACTCCTCCA+AGG - chr2.2:51575024-51575043 None:intergenic 35.0%
TCAAAGAAATTATGTGGAGG+CGG - chr2.2:51575248-51575267 None:intergenic 35.0%
TCATACTTCAACAACCACTT+TGG + chr2.2:51574723-51574742 MS.gene004636:CDS 35.0%
TCCCTAGCATACATAACATA+TGG - chr2.2:51575798-51575817 None:intergenic 35.0%
TTCTTGTGTGTGCATAATAG+TGG - chr2.2:51574239-51574258 None:intergenic 35.0%
TTGATTGGCATCATGAACTT+CGG - chr2.2:51575195-51575214 None:intergenic 35.0%
TTTACAGAGTGACATCAAAC+TGG - chr2.2:51574532-51574551 None:intergenic 35.0%
! GAAGTTGTCACTTGTAATGT+AGG + chr2.2:51574683-51574702 MS.gene004636:intron 35.0%
! TTATTTTCCTGTCTCCCTTT+TGG + chr2.2:51574103-51574122 MS.gene004636:intron 35.0%
AACATCAATGCCAAGATCGA+TGG - chr2.2:51575087-51575106 None:intergenic 40.0%
AACCAACAACAAGGCAAACA+TGG - chr2.2:51574372-51574391 None:intergenic 40.0%
AACGATGAAGATGTGGAAGA+TGG + chr2.2:51575290-51575309 MS.gene004636:CDS 40.0%
ACTCGTGCAACAAATCTTCT+TGG - chr2.2:51575582-51575601 None:intergenic 40.0%
ATCCTACTTACAGATGCCAT+GGG + chr2.2:51575627-51575646 MS.gene004636:CDS 40.0%
ATGTTTGCCTTCTTGTTGGT+TGG + chr2.2:51574182-51574201 MS.gene004636:intron 40.0%
GACTATGTTTGCCTTCTTGT+TGG + chr2.2:51574178-51574197 MS.gene004636:intron 40.0%
GCAGCATCAAAGAAGTTATG+CGG - chr2.2:51575329-51575348 None:intergenic 40.0%
GGATATCAACGATGAAGATG+TGG + chr2.2:51575283-51575302 MS.gene004636:CDS 40.0%
GGGTAACAATGGTGAAAACA+TGG + chr2.2:51575361-51575380 MS.gene004636:CDS 40.0%
GTCACTTGTAATGTAGGATG+AGG + chr2.2:51574689-51574708 MS.gene004636:intron 40.0%
GTGCAACAAATCTTCTTGGT+AGG - chr2.2:51575578-51575597 None:intergenic 40.0%
GTGGGAATGTGGCTAATAAT+GGG + chr2.2:51575390-51575409 MS.gene004636:CDS 40.0%
TAAGACACACTTACCTTGTC+TGG - chr2.2:51574013-51574032 None:intergenic 40.0%
TCATCATCCAACCAACAACA+AGG - chr2.2:51574381-51574400 None:intergenic 40.0%
TCATCATCCAACCAACAAGA+AGG - chr2.2:51574192-51574211 None:intergenic 40.0%
TGAAGCCATGCAAAATCTAC+CGG - chr2.2:51574132-51574151 None:intergenic 40.0%
TGATTGGCATCATGAACTTC+GGG - chr2.2:51575194-51575213 None:intergenic 40.0%
TGTCAGACTTGCAGAATACA+TGG + chr2.2:51575430-51575449 MS.gene004636:CDS 40.0%
TTATGTATGCTAGGGAAGCT+CGG + chr2.2:51575802-51575821 MS.gene004636:CDS 40.0%
! GGACCTTTTTCTATAGGCTA+AGG + chr2.2:51574826-51574845 MS.gene004636:CDS 40.0%
! GGTGTTTTCATAGGAAGTGA+GGG + chr2.2:51574798-51574817 MS.gene004636:CDS 40.0%
! GTGTTTTCATAGGAAGTGAG+GGG + chr2.2:51574799-51574818 MS.gene004636:CDS 40.0%
! GTTTTCACCATTGTTACCCA+TGG - chr2.2:51575360-51575379 None:intergenic 40.0%
! TGGTGTTTTCATAGGAAGTG+AGG + chr2.2:51574797-51574816 MS.gene004636:CDS 40.0%
!! AATTATGTGGAGGCGGATTT+TGG - chr2.2:51575241-51575260 None:intergenic 40.0%
!! AGAGATTTGTTACGGAGAAC+CGG + chr2.2:51575727-51575746 MS.gene004636:CDS 40.0%
!! AGATTTGTTGCACGAGTGTT+TGG + chr2.2:51575585-51575604 MS.gene004636:CDS 40.0%
!! ATGTTTGCCTTGTTGTTGGT+TGG + chr2.2:51574371-51574390 MS.gene004636:intron 40.0%
!! ATTATGTGGAGGCGGATTTT+GGG - chr2.2:51575240-51575259 None:intergenic 40.0%
!! GATTTGTTGCACGAGTGTTT+GGG + chr2.2:51575586-51575605 MS.gene004636:CDS 40.0%
!!! TCGAGGTTTGGTGTTTTCAT+AGG + chr2.2:51574789-51574808 MS.gene004636:CDS 40.0%
AATGGTGAAAACATGGGTGC+AGG + chr2.2:51575368-51575387 MS.gene004636:CDS 45.0%
AGACGCAGTATGAGACCTAA+CGG - chr2.2:51574876-51574895 None:intergenic 45.0%
ATCGTCGTCGTTGTCTTCAT+CGG - chr2.2:51575060-51575079 None:intergenic 45.0%
ATGAACTACGACACAGAGTC+TGG + chr2.2:51574748-51574767 MS.gene004636:CDS 45.0%
CACAACAATCGATCCAGACA+AGG + chr2.2:51573997-51574016 MS.gene004636:CDS 45.0%
CATCCTACTTACAGATGCCA+TGG + chr2.2:51575626-51575645 MS.gene004636:CDS 45.0%
CATGGCATCTGTAAGTAGGA+TGG - chr2.2:51575628-51575647 None:intergenic 45.0%
CATGGTGAAGACTAGCAATC+TGG + chr2.2:51575448-51575467 MS.gene004636:CDS 45.0%
CGTCTAAGTTACAACGGACA+GGG + chr2.2:51574891-51574910 MS.gene004636:CDS 45.0%
CTATAGGCTAAGGAGTTTGC+TGG + chr2.2:51574836-51574855 MS.gene004636:CDS 45.0%
GAACTTCCTGAATGTAAGCG+CGG - chr2.2:51573975-51573994 None:intergenic 45.0%
GACGATGATTCCATCGATCT+TGG + chr2.2:51575074-51575093 MS.gene004636:CDS 45.0%
GCATCAAAGAAGTTATGCGG+AGG - chr2.2:51575326-51575345 None:intergenic 45.0%
GCATCTGTAAGTAGGATGGT+GGG - chr2.2:51575624-51575643 None:intergenic 45.0%
GGCATTGTGTGGTTGTTGAT+TGG - chr2.2:51575210-51575229 None:intergenic 45.0%
GGTGGGAATGTGGCTAATAA+TGG + chr2.2:51575389-51575408 MS.gene004636:CDS 45.0%
GTCGTAGTTCATCACCAAAG+TGG - chr2.2:51574740-51574759 None:intergenic 45.0%
GTCTAAGTTACAACGGACAG+GGG + chr2.2:51574892-51574911 MS.gene004636:CDS 45.0%
GTCTGGTTAGAAGACACAAG+AGG + chr2.2:51574765-51574784 MS.gene004636:CDS 45.0%
GTGAAGACTAGCAATCTGGT+AGG + chr2.2:51575452-51575471 MS.gene004636:intron 45.0%
TAGAAGACACAAGAGGTTCG+AGG + chr2.2:51574772-51574791 MS.gene004636:CDS 45.0%
! GACCATGTTTGCCTTGTTGT+TGG + chr2.2:51574367-51574386 MS.gene004636:intron 45.0%
! GGTATCCGGTAGATTTTGCA+TGG + chr2.2:51574124-51574143 MS.gene004636:intron 45.0%
! TGTGGCTAATAATGGGAGAG+TGG + chr2.2:51575397-51575416 MS.gene004636:CDS 45.0%
!! CGGGTGGAAGAGATTTGTTA+CGG + chr2.2:51575719-51575738 MS.gene004636:CDS 45.0%
!! CTTTGATTTTGCTGCTGCCA+TGG + chr2.2:51575262-51575281 MS.gene004636:CDS 45.0%
!! TGGCAGTTGGGAAAAGTGTT+AGG + chr2.2:51575751-51575770 MS.gene004636:CDS 45.0%
ACATCAACATCCGCTGGATC+CGG - chr2.2:51575149-51575168 None:intergenic 50.0%
ACTCGACATGACCGAGCTAA+CGG + chr2.2:51574938-51574957 MS.gene004636:CDS 50.0%
ACTTTCCGTGCAGGATCAAG+TGG + chr2.2:51575655-51575674 MS.gene004636:CDS 50.0%
AGCCCATGGCATCTGTAAGT+AGG - chr2.2:51575632-51575651 None:intergenic 50.0%
AGGATCAAGTGGAAGGACAG+AGG + chr2.2:51575666-51575685 MS.gene004636:CDS 50.0%
ATCTCGGCAGCAATCAGGAA+AGG - chr2.2:51574993-51575012 None:intergenic 50.0%
CTCCAAGGGATCCAGTATCT+CGG - chr2.2:51575009-51575028 None:intergenic 50.0%
GACACAAGAGGTTCGAGGTT+TGG + chr2.2:51574777-51574796 MS.gene004636:CDS 50.0%
GCATCATGAACTTCGGGATG+AGG - chr2.2:51575188-51575207 None:intergenic 50.0%
GCGTCTAAGTTACAACGGAC+AGG + chr2.2:51574890-51574909 MS.gene004636:CDS 50.0%
GCTGTTGCCATGGGTAACAA+TGG + chr2.2:51575350-51575369 MS.gene004636:CDS 50.0%
GGCATCTGTAAGTAGGATGG+TGG - chr2.2:51575625-51575644 None:intergenic 50.0%
GTGAAAACATGGGTGCAGGT+GGG + chr2.2:51575372-51575391 MS.gene004636:CDS 50.0%
GTTGTCTTCATCGGATGACG+AGG - chr2.2:51575051-51575070 None:intergenic 50.0%
TCATGAACTTCGGGATGAGG+AGG - chr2.2:51575185-51575204 None:intergenic 50.0%
TCCTTCCACTTGATCCTGCA+CGG - chr2.2:51575663-51575682 None:intergenic 50.0%
TCGGGATGAGGAGGAGAATT+GGG - chr2.2:51575176-51575195 None:intergenic 50.0%
TTCAACTCCTGTGGCAGAAC+CGG + chr2.2:51575127-51575146 MS.gene004636:CDS 50.0%
TTCGGGATGAGGAGGAGAAT+TGG - chr2.2:51575177-51575196 None:intergenic 50.0%
! ATTTGTTACGGAGAACCGGC+TGG + chr2.2:51575731-51575750 MS.gene004636:CDS 50.0%
! CCTGTCTCCCTTTTGGTATC+CGG + chr2.2:51574110-51574129 MS.gene004636:intron 50.0%
! GGACGCGGACCTTTTTCTAT+AGG + chr2.2:51574820-51574839 MS.gene004636:CDS 50.0%
!! AGGCGGATTTTGGGCATCAT+CGG - chr2.2:51575231-51575250 None:intergenic 50.0%
!! TCTGTAAGTAGGATGGTGGG+CGG - chr2.2:51575621-51575640 None:intergenic 50.0%
CCAGTATCTCGGCAGCAATC+AGG - chr2.2:51574998-51575017 None:intergenic 55.0%
CCGGATACCAAAAGGGAGAC+AGG - chr2.2:51574113-51574132 None:intergenic 55.0%
CCTCTCACGAACCGTTAGCT+CGG - chr2.2:51574952-51574971 None:intergenic 55.0%
CCTGATTGCTGCCGAGATAC+TGG + chr2.2:51574995-51575014 MS.gene004636:CDS 55.0%
CGAGATACTGGATCCCTTGG+AGG + chr2.2:51575007-51575026 MS.gene004636:CDS 55.0%
CGGCAGCAATCAGGAAAGGA+GGG - chr2.2:51574989-51575008 None:intergenic 55.0%
GAAATGCCGCTGGAGGAAGA+TGG - chr2.2:51575692-51575711 None:intergenic 55.0%
GATGCTGCTGCTGTTGCCAT+GGG + chr2.2:51575341-51575360 MS.gene004636:CDS 55.0%
GCACGGAAAGTCAAAGCCCA+TGG - chr2.2:51575646-51575665 None:intergenic 55.0%
GCAGTATGAGACCTAACGGC+TGG - chr2.2:51574872-51574891 None:intergenic 55.0%
GGACAGGGGCTGTTCTACAT+GGG + chr2.2:51574906-51574925 MS.gene004636:CDS 55.0%
GGTGAAAACATGGGTGCAGG+TGG + chr2.2:51575371-51575390 MS.gene004636:CDS 55.0%
GGTGCAACATCAACATCCGC+TGG - chr2.2:51575155-51575174 None:intergenic 55.0%
GGTTCTGCCACAGGAGTTGA+AGG - chr2.2:51575128-51575147 None:intergenic 55.0%
GTATGAGACCTAACGGCTGG+TGG - chr2.2:51574869-51574888 None:intergenic 55.0%
GTATGCTAGGGAAGCTCGGA+TGG + chr2.2:51575806-51575825 MS.gene004636:CDS 55.0%
TCATAGGAAGTGAGGGGACG+CGG + chr2.2:51574805-51574824 MS.gene004636:CDS 55.0%
TCCGTGCAGGATCAAGTGGA+AGG + chr2.2:51575659-51575678 MS.gene004636:CDS 55.0%
TCCTCCAGCGGCATTTCTCA+GGG + chr2.2:51575695-51575714 MS.gene004636:CDS 55.0%
TCGGCAGCAATCAGGAAAGG+AGG - chr2.2:51574990-51575009 None:intergenic 55.0%
TCTGCCACAGGAGTTGAAGG+AGG - chr2.2:51575125-51575144 None:intergenic 55.0%
TGATGCTGCTGCTGTTGCCA+TGG + chr2.2:51575340-51575359 MS.gene004636:CDS 55.0%
TGCACCTCCTTCAACTCCTG+TGG + chr2.2:51575118-51575137 MS.gene004636:CDS 55.0%
TGCCGAGATACTGGATCCCT+TGG + chr2.2:51575004-51575023 MS.gene004636:CDS 55.0%
TGGGCATCATCGGCATTGTG+TGG - chr2.2:51575221-51575240 None:intergenic 55.0%
TTCCTCCAGCGGCATTTCTC+AGG + chr2.2:51575694-51575713 MS.gene004636:CDS 55.0%
! ACAGGAGTTGAAGGAGGTGC+AGG - chr2.2:51575119-51575138 None:intergenic 55.0%
! CACTTTTCCCAACTGCCAGC+CGG - chr2.2:51575749-51575768 None:intergenic 55.0%
! TGGGCTTTGACTTTCCGTGC+AGG + chr2.2:51575646-51575665 MS.gene004636:CDS 55.0%
CAGAGGCCATCTTCCTCCAG+CGG + chr2.2:51575683-51575702 MS.gene004636:CDS 60.0%
CATGGGTGCAGGTGGGAATG+TGG + chr2.2:51575379-51575398 MS.gene004636:CDS 60.0%
CCGAGCTAACGGTTCGTGAG+AGG + chr2.2:51574949-51574968 MS.gene004636:CDS 60.0%
CCTCCAGCGGCATTTCTCAG+GGG + chr2.2:51575696-51575715 MS.gene004636:CDS 60.0%
CGGACAGGGGCTGTTCTACA+TGG + chr2.2:51574905-51574924 MS.gene004636:CDS 60.0%
GAGCACTTCCACCAGCCGTT+AGG + chr2.2:51574858-51574877 MS.gene004636:CDS 60.0%
TGTGGCAGAACCGGATCCAG+CGG + chr2.2:51575136-51575155 MS.gene004636:CDS 60.0%
! GGAGTTGAAGGAGGTGCAGG+AGG - chr2.2:51575116-51575135 None:intergenic 60.0%
ACGGAGAACCGGCTGGCAGT+TGG + chr2.2:51575738-51575757 MS.gene004636:CDS 65.0%
CAGCGGCATTTCTCAGGGGC+GGG + chr2.2:51575700-51575719 MS.gene004636:CDS 65.0%
CCAGCGGCATTTCTCAGGGG+CGG + chr2.2:51575699-51575718 MS.gene004636:CDS 65.0%
CCCCTGAGAAATGCCGCTGG+AGG - chr2.2:51575699-51575718 None:intergenic 65.0%
CGGAGAACCGGCTGGCAGTT+GGG + chr2.2:51575739-51575758 MS.gene004636:CDS 65.0%
GCTGGATCCGGTTCTGCCAC+AGG - chr2.2:51575137-51575156 None:intergenic 65.0%
CCGCCCCTGAGAAATGCCGC+TGG - chr2.2:51575702-51575721 None:intergenic 70.0%
CGGCATTTCTCAGGGGCGGG+TGG + chr2.2:51575703-51575722 MS.gene004636:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 51573968 51575836 51573968 ID=MS.gene004636
chr2.2 mRNA 51573968 51575836 51573968 ID=MS.gene004636.t1;Parent=MS.gene004636
chr2.2 exon 51573968 51574018 51573968 ID=MS.gene004636.t1.exon1;Parent=MS.gene004636.t1
chr2.2 CDS 51573968 51574018 51573968 ID=cds.MS.gene004636.t1;Parent=MS.gene004636.t1
chr2.2 exon 51574705 51575469 51574705 ID=MS.gene004636.t1.exon2;Parent=MS.gene004636.t1
chr2.2 CDS 51574705 51575469 51574705 ID=cds.MS.gene004636.t1;Parent=MS.gene004636.t1
chr2.2 exon 51575573 51575836 51575573 ID=MS.gene004636.t1.exon3;Parent=MS.gene004636.t1
chr2.2 CDS 51575573 51575836 51575573 ID=cds.MS.gene004636.t1;Parent=MS.gene004636.t1
Gene Sequence

>MS.gene004636

ATGGCCGCGCTTACATTCAGGAAGTTCTTCACAACAATCGATCCAGACAAGGTAAGTGTGTCTTAATATTCACTAACTTTTGTTACTAAACTTTGAGTGTGGAGTAGATTTTTTAATCAAAAGTATGTTGATTGATTATTTTCCTGTCTCCCTTTTGGTATCCGGTAGATTTTGCATGGCTTCATTTGTTGTTACATGTTTAAGGATTTTGACTATGTTTGCCTTCTTGTTGGTTGGATGATGATGAATCTGTAAGTATAATTTATTTCCACTATTATGCACACACAAGAAACCGTATCTAAGATTTTGTGTACTCTGATTGTTAGATTGATAATTCCTGTTTGTTGTCACTCTGTAATGTTAGTTTGATATTTTCCTTGTCACTCTGCAATATATTTTGACCATGTTTGCCTTGTTGTTGGTTGGATGATGATGAATTGATAACTGTATTTTTTTCAACTATTATGCACACAAAAAAACCGTATCTACGATAATGTGTACTCTGATTGTTACTTTGATATTCCCAGTTTTTTGTCACTCTGTAATGTTAGTTTGAAATTTCCAGTTTGATGTCACTCTGTAAATCACATCGTCGTCAAATGACATCTCAATAAACATCAGTTGTATCTTAAGTAAATTATTTAAAAAAGAACACAGGTTTGTTATTTTCATGAGATATAGTATCTTTCAAATCATTTGTTGTAAAATGACTAATGAAGTTGTCACTTGTAATGTAGGATGAGGCTGATATTCCATCATACTTCAACAACCACTTTGGTGATGAACTACGACACAGAGTCTGGTTAGAAGACACAAGAGGTTCGAGGTTTGGTGTTTTCATAGGAAGTGAGGGGACGCGGACCTTTTTCTATAGGCTAAGGAGTTTGCTGGAGCACTTCCACCAGCCGTTAGGTCTCATACTGCGTCTAAGTTACAACGGACAGGGGCTGTTCTACATGGGTGCGCTTACACTCGACATGACCGAGCTAACGGTTCGTGAGAGGCGTACTTATGAACACCCTCCTTTCCTGATTGCTGCCGAGATACTGGATCCCTTGGAGGAGTATGAATTATACAATACCTCGTCATCCGATGAAGACAACGACGACGATGATTCCATCGATCTTGGCATTGATGTTGATGTTCCTCCTGCACCTCCTTCAACTCCTGTGGCAGAACCGGATCCAGCGGATGTTGATGTTGCACCCAATTCTCCTCCTCATCCCGAAGTTCATGATGCCAATCAACAACCACACAATGCCGATGATGCCCAAAATCCGCCTCCACATAATTTCTTTGATTTTGCTGCTGCCATGGATATCAACGATGAAGATGTGGAAGATGGAGCACAAAATCCTCCGCATAACTTCTTTGATGCTGCTGCTGTTGCCATGGGTAACAATGGTGAAAACATGGGTGCAGGTGGGAATGTGGCTAATAATGGGAGAGTGGAGAGCTGTGTTGTCAGACTTGCAGAATACATGGTGAAGACTAGCAATCTGGTAGGTGAATTGCATAACTGCTAATGCATAATGTAATTACTGTTATATAGTTTTTGGTAGATATGTCTAACGCCTGTTTTAAATTGTTATCATTTGCATGCAGTACCTACCAAGAAGATTTGTTGCACGAGTGTTTGGGAATGCAACACCGCCCACCATCCTACTTACAGATGCCATGGGCTTTGACTTTCCGTGCAGGATCAAGTGGAAGGACAGAGGCCATCTTCCTCCAGCGGCATTTCTCAGGGGCGGGTGGAAGAGATTTGTTACGGAGAACCGGCTGGCAGTTGGGAAAAGTGTTAGGTTGTCTGTCAACTTAGACAACCCATATGTTATGTATGCTAGGGAAGCTCGGATGGAGATTTGA
Protein sequence

>MS.gene004636.t1

MAALTFRKFFTTIDPDKDEADIPSYFNNHFGDELRHRVWLEDTRGSRFGVFIGSEGTRTFFYRLRSLLEHFHQPLGLILRLSYNGQGLFYMGALTLDMTELTVRERRTYEHPPFLIAAEILDPLEEYELYNTSSSDEDNDDDDSIDLGIDVDVPPAPPSTPVAEPDPADVDVAPNSPPHPEVHDANQQPHNADDAQNPPPHNFFDFAAAMDINDEDVEDGAQNPPHNFFDAAAVAMGNNGENMGAGGNVANNGRVESCVVRLAEYMVKTSNLYLPRRFVARVFGNATPPTILLTDAMGFDFPCRIKWKDRGHLPPAAFLRGGWKRFVTENRLAVGKSVRLSVNLDNPYVMYAREARMEI