AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004646

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004646.t1 MTR_2g437200 73.077 130 27 2 1 129 1 123 6.19e-51 184
MS.gene004646.t1 MTR_2g437240 67.832 143 37 3 1 143 1 134 1.45e-49 180
MS.gene004646.t1 MTR_2g437260 72.868 129 31 2 1 128 1 126 3.03e-49 179
MS.gene004646.t1 MTR_2g438020 87.500 104 13 0 27 130 60 163 8.99e-47 174
MS.gene004646.t1 MTR_2g437120 48.276 145 59 3 14 149 20 157 2.80e-31 127
MS.gene004646.t1 MTR_2g437040 52.308 130 52 4 22 149 25 146 2.42e-30 124
MS.gene004646.t1 MTR_2g437060 57.798 109 43 2 22 128 40 147 1.41e-29 122
MS.gene004646.t1 MTR_2g437130 52.713 129 54 3 2 130 14 135 1.57e-29 122
MS.gene004646.t1 MTR_2g436370 49.167 120 52 3 32 149 40 152 1.91e-28 114
MS.gene004646.t1 MTR_2g437160 50.355 141 55 4 3 128 15 155 3.55e-28 118
MS.gene004646.t1 MTR_2g437150 46.358 151 61 5 14 151 20 163 6.61e-28 117
MS.gene004646.t1 MTR_2g437030 46.897 145 67 5 25 167 114 250 8.04e-28 118
MS.gene004646.t1 MTR_2g436460 58.163 98 41 0 31 128 40 137 9.43e-28 116
MS.gene004646.t1 MTR_2g076320 50.000 144 57 4 14 149 20 156 1.35e-27 116
MS.gene004646.t1 MTR_2g437990 50.000 144 57 4 14 149 20 156 1.43e-27 117
MS.gene004646.t1 MTR_2g437170 46.479 142 61 4 18 151 29 163 2.32e-27 115
MS.gene004646.t1 MTR_2g437020 47.761 134 60 4 18 149 20 145 3.47e-27 114
MS.gene004646.t1 MTR_4g119550 58.163 98 40 1 34 130 47 144 5.95e-27 114
MS.gene004646.t1 MTR_3g085240 63.441 93 31 2 1 92 21 111 9.89e-27 110
MS.gene004646.t1 MTR_2g437080 52.727 110 49 2 21 128 24 132 1.06e-26 113
MS.gene004646.t1 MTR_2g436440 54.717 106 45 2 25 128 34 138 1.80e-26 112
MS.gene004646.t1 MTR_2g437940 49.630 135 56 3 3 125 4 138 4.42e-26 111
MS.gene004646.t1 MTR_2g437180 50.877 114 54 1 17 128 28 141 9.22e-26 110
MS.gene004646.t1 MTR_2g437100 42.169 166 72 4 3 151 10 168 9.81e-26 110
MS.gene004646.t1 MTR_4g049400 44.755 143 66 3 1 130 2 144 1.54e-25 106
MS.gene004646.t1 MTR_2g019840 47.934 121 62 1 8 128 14 133 2.00e-24 106
MS.gene004646.t1 MTR_2g438010 48.000 125 57 2 14 130 40 164 4.21e-24 106
MS.gene004646.t1 MTR_2g437960 53.684 95 42 1 38 130 53 147 7.40e-24 105
MS.gene004646.t1 MTR_2g437960 53.684 95 42 1 38 130 53 147 7.56e-24 105
MS.gene004646.t1 MTR_2g436400 50.505 99 47 1 32 128 41 139 1.48e-23 103
MS.gene004646.t1 MTR_4g120380 55.102 98 44 0 31 128 37 134 2.82e-23 103
MS.gene004646.t1 MTR_4g120380 55.102 98 44 0 31 128 37 134 6.07e-23 102
MS.gene004646.t1 MTR_7g078980 40.397 151 31 3 318 468 20 111 1.94e-21 90.5
MS.gene004646.t1 MTR_4g119580 50.000 106 52 1 24 128 29 134 1.30e-20 95.1
MS.gene004646.t1 MTR_2g019810 45.763 118 59 1 11 128 9 121 2.33e-20 94.4
MS.gene004646.t1 MTR_2g437160 45.312 128 55 4 3 115 15 142 8.95e-20 91.7
MS.gene004646.t1 MTR_7g078930 76.364 55 13 0 414 468 75 129 1.22e-18 83.2
MS.gene004646.t1 MTR_1g055005 43.299 97 51 2 686 780 47 141 1.89e-13 70.1
MS.gene004646.t1 MTR_3g028350 37.500 88 30 2 499 586 43 105 3.21e-11 62.4
MS.gene004646.t1 MTR_4g007490 38.202 89 55 0 40 128 39 127 6.97e-11 65.5
MS.gene004646.t1 MTR_3g450500 35.238 105 68 0 24 128 24 128 7.17e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004646.t1 AT5G33406 47.802 364 186 2 432 792 1 363 4.45e-115 358
MS.gene004646.t1 AT4G15020 32.466 653 401 11 131 747 64 712 2.09e-101 331
MS.gene004646.t1 AT4G15020 32.466 653 401 11 131 747 64 712 2.09e-101 331
MS.gene004646.t1 AT3G22220 35.104 527 331 7 216 737 177 697 5.03e-97 319
MS.gene004646.t1 AT3G22220 35.104 527 331 7 216 737 177 697 5.03e-97 319
MS.gene004646.t1 AT3G22220 35.104 527 331 7 216 737 177 697 5.03e-97 319
MS.gene004646.t1 AT3G22220 35.104 527 331 7 216 737 177 697 5.03e-97 319
MS.gene004646.t1 AT1G79740 33.397 527 323 9 232 751 112 617 1.13e-92 304
MS.gene004646.t1 AT1G79740 33.397 527 323 9 232 751 112 617 1.13e-92 304
MS.gene004646.t1 AT3G17450 32.389 494 330 4 224 714 298 790 8.06e-91 305
MS.gene004646.t1 AT3G17450 32.389 494 330 4 224 714 298 790 8.06e-91 305
MS.gene004646.t1 AT1G79740 32.871 505 311 9 254 751 1 484 5.31e-87 285
MS.gene004646.t1 AT1G79740 32.871 505 311 9 254 751 1 484 5.31e-87 285
MS.gene004646.t1 AT3G13020 30.350 514 338 11 207 712 100 601 1.34e-65 230
MS.gene004646.t1 AT3G13030 30.769 468 308 8 251 712 72 529 1.32e-62 220
MS.gene004646.t1 AT3G13030 30.769 468 308 8 251 712 72 529 1.32e-62 220
MS.gene004646.t1 AT3G13030 30.769 468 308 8 251 712 72 529 1.32e-62 220
MS.gene004646.t1 AT3G13030 30.769 468 308 8 251 712 72 529 1.32e-62 220
MS.gene004646.t1 AT3G13030 28.970 466 286 8 251 712 72 496 5.82e-54 195
MS.gene004646.t1 AT1G43260 35.776 232 148 1 230 460 37 268 9.05e-48 172
MS.gene004646.t1 AT3G13010 25.856 526 358 12 191 712 71 568 2.86e-44 169
MS.gene004646.t1 AT1G36095 40.741 162 96 0 340 501 137 298 1.80e-32 128
MS.gene004646.t1 AT5G31412 41.985 131 74 1 281 411 116 244 2.79e-25 110
MS.gene004646.t1 AT3G46950 37.398 123 71 3 11 128 20 141 3.08e-17 85.9
MS.gene004646.t1 AT1G61980 39.362 94 57 0 40 133 53 146 3.22e-16 82.4
MS.gene004646.t1 AT1G61980 39.362 94 57 0 40 133 53 146 3.22e-16 82.4
MS.gene004646.t1 AT1G61980 39.362 94 57 0 40 133 53 146 3.22e-16 82.4
MS.gene004646.t1 AT1G61970 32.468 154 94 2 38 183 51 202 4.13e-16 82.0
MS.gene004646.t1 AT1G61970 32.468 154 94 2 38 183 51 202 4.13e-16 82.0
MS.gene004646.t1 AT1G61970 32.468 154 94 2 38 183 51 202 4.13e-16 82.0
MS.gene004646.t1 AT1G61970 32.468 154 94 2 38 183 51 202 4.13e-16 82.0
MS.gene004646.t1 AT1G61970 32.468 154 94 2 38 183 51 202 4.13e-16 82.0
MS.gene004646.t1 AT1G61970 32.468 154 94 2 38 183 51 202 4.13e-16 82.0
MS.gene004646.t1 AT1G61970 32.468 154 94 2 38 183 51 202 4.13e-16 82.0
MS.gene004646.t1 AT1G62120 36.000 125 68 1 40 152 57 181 7.24e-16 81.3
MS.gene004646.t1 AT1G62085 42.697 89 51 0 40 128 56 144 3.41e-15 79.3
MS.gene004646.t1 AT1G62085 42.697 89 51 0 40 128 56 144 3.41e-15 79.3
MS.gene004646.t1 AT5G07900 33.607 122 67 2 23 130 39 160 1.95e-14 76.6
MS.gene004646.t1 AT1G61960 38.542 96 59 0 38 133 51 146 5.21e-14 75.9
MS.gene004646.t1 AT1G21150 41.758 91 53 0 40 130 57 147 9.40e-14 74.3
MS.gene004646.t1 AT1G21150 41.489 94 55 0 40 133 121 214 1.65e-13 73.9
MS.gene004646.t1 AT1G21150 38.462 104 64 0 30 133 11 114 1.86e-13 73.2
MS.gene004646.t1 AT1G61990 40.449 89 53 0 40 128 53 141 1.89e-13 73.6
MS.gene004646.t1 AT1G61990 40.449 89 53 0 40 128 53 141 1.89e-13 73.6
MS.gene004646.t1 AT1G62150 35.165 91 59 0 38 128 57 147 4.55e-12 69.7
MS.gene004646.t1 AT1G62110 36.170 94 60 0 40 133 53 146 8.52e-12 68.9
MS.gene004646.t1 AT1G62870 21.333 450 303 15 303 714 293 729 3.42e-11 67.4
MS.gene004646.t1 AT5G23930 34.454 119 68 1 25 133 31 149 8.38e-11 65.5

Find 166 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAGTTTACTTCTCTTCT+TGG 0.123186 2.2:-51526675 None:intergenic
TCCTTTGGATGTATTGTTTA+TGG 0.124682 2.2:+51525021 MS.gene004646:CDS
TTGGGATCGTTCTCAATTTC+TGG 0.141294 2.2:-51526195 None:intergenic
ATGCAAACCTCTCAGCAATT+TGG 0.171724 2.2:-51526214 None:intergenic
GTGCACTTCTGCCATCATTT+TGG 0.194903 2.2:+51525911 MS.gene004646:CDS
CACCAAGCTATTATGAAATT+AGG 0.200102 2.2:+51525233 MS.gene004646:CDS
AGGCGTGCTGGAGGATTATT+TGG 0.210829 2.2:+51526306 MS.gene004646:CDS
TGAACACAAGGGCTAGTATT+AGG 0.241356 2.2:+51526939 MS.gene004646:CDS
CAAAGGACCTTTCTTGGCTT+TGG 0.244193 2.2:-51525005 None:intergenic
GACATTGTCCGCATGGTTAA+AGG 0.256602 2.2:+51520057 MS.gene004646:CDS
TATCATACTGAATGGTTAAC+AGG 0.259479 2.2:+51526803 MS.gene004646:CDS
GGTTTGCTATAAAAGTATTT+AGG 0.266192 2.2:-51526174 None:intergenic
ACCAAGCTATTATGAAATTA+GGG 0.272366 2.2:+51525234 MS.gene004646:CDS
CCTTTGGATGTATTGTTTAT+GGG 0.278807 2.2:+51525022 MS.gene004646:CDS
TAAGATTCCTAGGATTAAAA+AGG 0.286245 2.2:+51525645 MS.gene004646:CDS
TACATCCAAAGGACCTTTCT+TGG 0.292962 2.2:-51525011 None:intergenic
GGCATTGTTGCAAGCTGATT+TGG 0.294913 2.2:+51524850 MS.gene004646:CDS
AGAACAAACCTCACATCTTT+TGG 0.312210 2.2:+51525575 MS.gene004646:CDS
AAGAAGAAATTGAAGAAATA+AGG 0.315176 2.2:+51524936 MS.gene004646:CDS
AAAGGACCTTTCTTGGCTTT+GGG 0.317669 2.2:-51525004 None:intergenic
TTGGAAGCTGTTGGAGCTTA+TGG 0.320306 2.2:+51525196 MS.gene004646:CDS
AGTTTGGTTTGTAGTGCTTC+AGG 0.326354 2.2:+51526521 MS.gene004646:CDS
TTGGCCGCAAGGGTTAAATA+TGG 0.342154 2.2:+51525310 MS.gene004646:CDS
CTTCAGGATGTGAGCGCAAC+TGG 0.351319 2.2:+51526537 MS.gene004646:CDS
TGCAAACCTCTCAGCAATTT+GGG 0.359854 2.2:-51526213 None:intergenic
GAAGATGTTGGTGCCTCATT+TGG 0.361160 2.2:+51525291 MS.gene004646:CDS
GTCAGATTCAGAAGCACCTT+TGG 0.364937 2.2:-51520037 None:intergenic
CAACTCAGTTGGGACAATTC+AGG 0.365284 2.2:+51526286 MS.gene004646:CDS
GCTGAGTGGTGGATGAATTA+TGG 0.375891 2.2:+51526455 MS.gene004646:intron
TCATGAACAAATTCTTCAAC+AGG 0.376778 2.2:-51526857 None:intergenic
CAAAAGGGGTGTTCTCTTGT+AGG 0.377000 2.2:+51525673 MS.gene004646:CDS
GAAGAAGTTGATAACTGAAT+CGG 0.380840 2.2:-51519902 None:intergenic
AACCAAACTCAGCACTTTGA+TGG 0.386442 2.2:-51526507 None:intergenic
GGCCATCAAAGTGCTGAGTT+TGG 0.386630 2.2:+51526505 MS.gene004646:CDS
TCCTACCTCATTGACAAATT+CGG 0.388145 2.2:+51519817 MS.gene004646:CDS
GTTTGAAGTGGTGGTGGTGG+TGG 0.393771 2.2:-51519782 None:intergenic
GACCTCATGTTGGAAGATAT+TGG 0.394320 2.2:+51525622 MS.gene004646:CDS
CTAGCCCTTGTGTTCACAAT+AGG 0.401254 2.2:-51526932 None:intergenic
TGGGTTTGAAGTGGTGGTGG+TGG 0.404259 2.2:-51519785 None:intergenic
AATGTTTGTTTGCCTAGTAC+TGG 0.405864 2.2:-51525062 None:intergenic
GTTGAGTTCCAAGTGCAATA+AGG 0.408152 2.2:+51525861 MS.gene004646:CDS
AACAGGGGAGATGGGAGAGA+TGG 0.413136 2.2:+51526820 MS.gene004646:CDS
ATGGGAGAGATGGGAGAGGA+TGG 0.413573 2.2:+51526830 MS.gene004646:CDS
CATATCGGTCTTTCAAAGCT+TGG 0.413634 2.2:-51526736 None:intergenic
AAGATATTGGTAAGATTCCT+AGG 0.417549 2.2:+51525635 MS.gene004646:CDS
GCATATATTTCACCCATTGC+AGG 0.418557 2.2:-51526009 None:intergenic
ACAGGGGAGATGGGAGAGAT+GGG 0.421275 2.2:+51526821 MS.gene004646:CDS
TGAGGAATTTGAGAGAAAAT+AGG 0.422691 2.2:-51519759 None:intergenic
CAAAGCCAAGAAAGGTCCTT+TGG 0.422936 2.2:+51525006 MS.gene004646:CDS
GAACACAAGGGCTAGTATTA+GGG 0.424051 2.2:+51526940 MS.gene004646:CDS
CTCATTAACCCCACTTGCAT+TGG 0.431159 2.2:-51526909 None:intergenic
TCAAAATCATTGATAAAAGA+TGG 0.443562 2.2:+51526103 MS.gene004646:CDS
ATGAAAATGATGATCTAACT+TGG 0.444991 2.2:+51526876 MS.gene004646:CDS
CTTTGGATGTATTGTTTATG+GGG 0.445568 2.2:+51525023 MS.gene004646:CDS
TAAAACACCAGCAGAGGTTA+AGG 0.447094 2.2:+51524829 MS.gene004646:CDS
AAAGAAATGTTGGAAGCTGT+TGG 0.449680 2.2:+51525187 MS.gene004646:CDS
CATCATCCATTGCATGCTAC+TGG 0.451137 2.2:+51526138 MS.gene004646:CDS
AAGAAGTTGATAACTGAATC+GGG 0.461552 2.2:-51519901 None:intergenic
GAAATAAGGAGAATTCGAAC+TGG 0.469581 2.2:+51524950 MS.gene004646:CDS
ACATACGCAATATCATCAAA+AGG 0.472800 2.2:+51519950 MS.gene004646:CDS
GACTTGGTTACAACTCAGTT+GGG 0.475045 2.2:+51526276 MS.gene004646:CDS
GAATCGAGCAATAAATTGTA+TGG 0.476231 2.2:-51525116 None:intergenic
CCCCTAATTTCATAATAGCT+TGG 0.478373 2.2:-51525235 None:intergenic
TTGGGACAATTCAGGCGTGC+TGG 0.484786 2.2:+51526294 MS.gene004646:CDS
AACAATGCCTTAACCTCTGC+TGG 0.488665 2.2:-51524836 None:intergenic
CATCATCATCTATTTGCGCT+TGG 0.489201 2.2:-51524910 None:intergenic
AAAACACTTCAAAGAAATGT+TGG 0.489376 2.2:+51525177 MS.gene004646:CDS
AACTCCGGATTTGACACTAA+TGG 0.490085 2.2:+51526484 MS.gene004646:CDS
CTAATTTCATAATAGCTTGG+TGG 0.490281 2.2:-51525232 None:intergenic
AGAGTCAAAGACTGGAGAAA+TGG 0.491539 2.2:+51525444 MS.gene004646:CDS
AATATCATCAAAAGGGTACC+AGG 0.494005 2.2:+51519958 MS.gene004646:CDS
TTTAGCTTCCTTATTGCACT+TGG 0.494175 2.2:-51525869 None:intergenic
TATAAAAGTATTTAGGATTG+AGG 0.495869 2.2:-51526167 None:intergenic
AGACTTGGTTACAACTCAGT+TGG 0.497240 2.2:+51526275 MS.gene004646:CDS
ATGAGGTAGGATACGATGAA+TGG 0.499551 2.2:-51519805 None:intergenic
TAAGGAAGCTAAAGGTAGGC+AGG 0.502654 2.2:+51525879 MS.gene004646:CDS
GGAGATGGGAGAGATGGGAG+AGG 0.503278 2.2:+51526826 MS.gene004646:CDS
TGAATGGTTAACAGGGGAGA+TGG 0.505332 2.2:+51526811 MS.gene004646:CDS
TCTTGTTGACGTGCTAAGAA+TGG 0.508954 2.2:+51525969 MS.gene004646:CDS
TCCAACTCTATTTCAAGTAG+TGG 0.509907 2.2:-51525259 None:intergenic
GAAAACTAACAATGTTGAGT+TGG 0.510891 2.2:+51525738 MS.gene004646:CDS
TGTTTGCCTAGTACTGGTAC+TGG 0.511214 2.2:-51525056 None:intergenic
ATGGCCATTAGTGTCAAATC+CGG 0.512170 2.2:-51526488 None:intergenic
TTAACCCTTGCGGCCAAATG+AGG 0.512197 2.2:-51525304 None:intergenic
TTGTTCCTTCGCATTATCCA+TGG 0.512640 2.2:-51526034 None:intergenic
TGGTGCCTCATTTGGCCGCA+AGG 0.512671 2.2:+51525299 MS.gene004646:CDS
TTAAAATCATACAACATCAC+GGG 0.516976 2.2:-51526779 None:intergenic
GAATGGGTTTGAAGTGGTGG+TGG 0.518044 2.2:-51519788 None:intergenic
AGACTACCAGTACCAGTACT+AGG 0.519101 2.2:+51525050 MS.gene004646:CDS
GCTTCTGAAGAGTCAAAGAC+TGG 0.521479 2.2:+51525436 MS.gene004646:CDS
GCATCAGAAGTTGCAAGAGT+TGG 0.523127 2.2:+51526697 MS.gene004646:CDS
TGAGGTAGGATACGATGAAT+GGG 0.529282 2.2:-51519804 None:intergenic
CCACATTTCTTACTTTGCAA+AGG 0.529530 2.2:+51525788 MS.gene004646:CDS
CATTTGCTTTGAGCCTAATG+AGG 0.529987 2.2:+51525713 MS.gene004646:CDS
GGAAGCAACTATGTTTATGC+TGG 0.533778 2.2:+51525538 MS.gene004646:CDS
AGTACATCATTCCAAAATGA+TGG 0.537042 2.2:-51525922 None:intergenic
CAAAATCATTGATAAAAGAT+GGG 0.542128 2.2:+51526104 MS.gene004646:CDS
ATTAAAATCATACAACATCA+CGG 0.545758 2.2:-51526780 None:intergenic
TCTTGTCAAGACAATCATAT+CGG 0.545836 2.2:-51526751 None:intergenic
TCTGCTCCCAAAGCCAAGAA+AGG 0.546960 2.2:+51524998 MS.gene004646:CDS
CTACGGTCAAAACTGTCTCC+AGG 0.547728 2.2:+51526348 MS.gene004646:CDS
TGGAGGATTATTTGGAGGAA+AGG 0.548496 2.2:+51526314 MS.gene004646:CDS
CATACGCAATATCATCAAAA+GGG 0.549145 2.2:+51519951 MS.gene004646:CDS
GGTGCCTCATTTGGCCGCAA+GGG 0.550001 2.2:+51525300 MS.gene004646:CDS
AAGTGCAATAAGGAAGCTAA+AGG 0.551734 2.2:+51525871 MS.gene004646:CDS
GAATGGTTAACAGGGGAGAT+GGG 0.552162 2.2:+51526812 MS.gene004646:CDS
CGTGCTGGAGGATTATTTGG+AGG 0.553023 2.2:+51526309 MS.gene004646:CDS
ACCACTACTTGAAATAGAGT+TGG 0.558521 2.2:+51525258 MS.gene004646:CDS
GCAATAAGGAAGCTAAAGGT+AGG 0.563127 2.2:+51525875 MS.gene004646:CDS
CACGTCAACAAGAGGTGCCA+TGG 0.566526 2.2:-51525959 None:intergenic
GGAAGAAACTAAGAAGATGT+TGG 0.566866 2.2:+51525279 MS.gene004646:CDS
CCCATAAACAATACATCCAA+AGG 0.570419 2.2:-51525022 None:intergenic
TTGGAGGAAAGGCAGCAGTA+AGG 0.571341 2.2:+51526325 MS.gene004646:CDS
TGAATCTGACATTGTCCGCA+TGG 0.573592 2.2:+51520050 MS.gene004646:CDS
GGGTGAAATATATGCAGCCA+TGG 0.574934 2.2:+51526017 MS.gene004646:CDS
TGAAAATGATGATCTAACTT+GGG 0.580403 2.2:+51526877 MS.gene004646:CDS
GCTCGATTATGTCCGATGGT+TGG 0.585062 2.2:+51525335 MS.gene004646:CDS
GGAGAATTCGAACTGGAAAG+AGG 0.585181 2.2:+51524957 MS.gene004646:CDS
AATGTTGAGTTGGTTAAACA+TGG 0.586870 2.2:+51525748 MS.gene004646:CDS
GAAAGGCAGCAGTAAGGCTA+CGG 0.587094 2.2:+51526331 MS.gene004646:CDS
CATGCAATGGATGATGAAGT+TGG 0.588948 2.2:-51526131 None:intergenic
GAATTATGGAGCAGAAACTC+CGG 0.589304 2.2:+51526469 MS.gene004646:CDS
TCTATGCAGTGTGCAGCACA+TGG 0.592868 2.2:-51525601 None:intergenic
GTATTGCAGGAAAGAATCCC+TGG 0.593025 2.2:-51519976 None:intergenic
GGCTTTGGGAGCAGACAAAG+AGG 0.593580 2.2:-51524990 None:intergenic
ATCAAGTTGCCAATGCAAGT+GGG 0.598161 2.2:+51526900 MS.gene004646:CDS
ATGTAATGAAGTGGCAGACT+TGG 0.599387 2.2:+51526260 MS.gene004646:CDS
GATCAAGTTGCCAATGCAAG+TGG 0.601135 2.2:+51526899 MS.gene004646:CDS
GGACAATTCAGGCGTGCTGG+AGG 0.604827 2.2:+51526297 MS.gene004646:CDS
ATGAGCCTATTGTGAACACA+AGG 0.604851 2.2:+51526927 MS.gene004646:CDS
GATGGTTGGACAGACCGAAA+AGG 0.605006 2.2:+51525349 MS.gene004646:CDS
TTTGGATGTATTGTTTATGG+GGG 0.605885 2.2:+51525024 MS.gene004646:CDS
ATCATACTGAATGGTTAACA+GGG 0.605897 2.2:+51526804 MS.gene004646:CDS
TGGTTAAACATGGAGTCACA+AGG 0.606856 2.2:+51525758 MS.gene004646:CDS
TAGTAACCAGTAGCATGCAA+TGG 0.607605 2.2:-51526144 None:intergenic
ATTCTTAGCACGTCAACAAG+AGG 0.615152 2.2:-51525967 None:intergenic
TCCGAATTTGTCAATGAGGT+AGG 0.619489 2.2:-51519818 None:intergenic
ACTCCACACTCTTAAGTCCA+TGG 0.620572 2.2:+51525942 MS.gene004646:CDS
GCATCCATATTTAACCCTTG+CGG 0.623330 2.2:-51525314 None:intergenic
TATTAGGGCAGAACGAGCAA+AGG 0.625543 2.2:+51526955 MS.gene004646:CDS
TACCAATATCTTCCAACATG+AGG 0.628099 2.2:-51525624 None:intergenic
GCAGACAAAGAGGAATCAGA+TGG 0.633382 2.2:-51524980 None:intergenic
GATGAATGGGTTTGAAGTGG+TGG 0.633822 2.2:-51519791 None:intergenic
TGCAGCCATGGATAATGCGA+AGG 0.634019 2.2:+51526029 MS.gene004646:CDS
AATTTGAGAGAAAATAGGAA+AGG 0.640380 2.2:-51519754 None:intergenic
AGAATCCGAATTTGTCAATG+AGG 0.640871 2.2:-51519822 None:intergenic
TCAAGTTGCCAATGCAAGTG+GGG 0.642208 2.2:+51526901 MS.gene004646:CDS
ATGTCGTAAAACACCAGCAG+AGG 0.643636 2.2:+51524823 MS.gene004646:CDS
TTAGGCTCAAAGCAAATGTG+TGG 0.655077 2.2:-51525708 None:intergenic
TACGATGAATGGGTTTGAAG+TGG 0.656177 2.2:-51519794 None:intergenic
GTGCAATTGATTACTGACAA+TGG 0.657518 2.2:+51525517 MS.gene004646:CDS
GGATGCTCGATTATGTCCGA+TGG 0.663540 2.2:+51525331 MS.gene004646:CDS
GAAATCAGAAGGTATTGCAG+AGG 0.665270 2.2:+51524886 MS.gene004646:CDS
GTGCCATGGACTTAAGAGTG+TGG 0.673069 2.2:-51525945 None:intergenic
ACTTTGTGAATGTAATGAAG+TGG 0.673382 2.2:+51526251 MS.gene004646:CDS
AACGATCCCAAATTGCTGAG+AGG 0.673811 2.2:+51526207 MS.gene004646:CDS
CATGGAGTCCAAAAGATGTG+AGG 0.680155 2.2:-51525583 None:intergenic
CCAAGCTATTATGAAATTAG+GGG 0.681715 2.2:+51525235 MS.gene004646:CDS
ATATCATCAAAAGGGTACCA+GGG 0.689286 2.2:+51519959 MS.gene004646:CDS
CCTTTGCAAAGTAAGAAATG+TGG 0.689473 2.2:-51525788 None:intergenic
TAAAATCATACAACATCACG+GGG 0.706865 2.2:-51526778 None:intergenic
TGAGATGGGTGAGCAAAATG+TGG 0.709212 2.2:+51525495 MS.gene004646:CDS
ACACTGCATAGACCTCATGT+TGG 0.712729 2.2:+51525612 MS.gene004646:CDS
TCATACTGAATGGTTAACAG+GGG 0.727022 2.2:+51526805 MS.gene004646:CDS
GAGGGTTACCTTTAACCATG+CGG 0.736609 2.2:-51520065 None:intergenic
TGAGCCTATTGTGAACACAA+GGG 0.750296 2.2:+51526928 MS.gene004646:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.2 gene 51519691 51527208 51519691 ID=MS.gene004646
chr2.2 mRNA 51519691 51527208 51519691 ID=MS.gene004646.t1;Parent=MS.gene004646
chr2.2 exon 51519691 51520078 51519691 ID=MS.gene004646.t1.exon1;Parent=MS.gene004646.t1
chr2.2 CDS 51519691 51520078 51519691 ID=cds.MS.gene004646.t1;Parent=MS.gene004646.t1
chr2.2 exon 51524822 51526369 51524822 ID=MS.gene004646.t1.exon2;Parent=MS.gene004646.t1
chr2.2 CDS 51524822 51526369 51524822 ID=cds.MS.gene004646.t1;Parent=MS.gene004646.t1
chr2.2 exon 51526456 51526574 51526456 ID=MS.gene004646.t1.exon3;Parent=MS.gene004646.t1
chr2.2 CDS 51526456 51526574 51526456 ID=cds.MS.gene004646.t1;Parent=MS.gene004646.t1
chr2.2 exon 51526668 51526995 51526668 ID=MS.gene004646.t1.exon4;Parent=MS.gene004646.t1
chr2.2 CDS 51526668 51526995 51526668 ID=cds.MS.gene004646.t1;Parent=MS.gene004646.t1
chr2.2 exon 51527189 51527208 51527189 ID=MS.gene004646.t1.exon5;Parent=MS.gene004646.t1
chr2.2 CDS 51527189 51527208 51527189 ID=cds.MS.gene004646.t1;Parent=MS.gene004646.t1
Gene Sequence

>MS.gene004646

ATGTTCAAAGCTTTTCTCTGCAACAATGCATTTCGCATATCAACAACAACACTCTACAACGCACCTTTCCTATTTTCTCTCAAATTCCTCACCACCACCACCACCACTTCAAACCCATTCATCGTATCCTACCTCATTGACAAATTCGGATTCTCACATGAATTCGCACTCAAAGCTTCCAAAGAGCTTTATTTCAAAACCTCACAAAAACCCGATTCAGTTATCAACTTCTTCAAAAATCATGGTTTCAATGACTCAGACATACGCAATATCATCAAAAGGGTACCAGGGATTCTTTCCTGCAATACCCACAAAACGATTCTCCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTGAATCTGACATTGTCCGCATGGTTAAAGGTAACCCTCAGTTCTTGCAATTAAGCTTGAAGACTCATGAAATTAAGTTTCAATATTTTCTATCCAAAGGTGCTTCTTCTTCTGATATTGTTTCATTGTTAACTGCAAACCCTCAAATTTTACAAAGTAGCTTAGACAAACGAATAATCCCTCTTTTTGAATTATTAAGTAGGTTCTTGAAATCCAGCAAGGATACGATTCTCTGCTTAATTCGATATTCTGCTGCATTTACTATAAATAATCCCTATCGCCTTATTGTGGCTAATATCAATTTGATGACTGATTTTGGAGTTTCTGATAATGTCATTGCTAGTATGCTTCAGTCAGAGCCAACTGTATTTGGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGTTTTAGGGTTTCATCCTTCAAAGACTACTTTCGGAATTGCTTTGGCAGTAAAGAAAGGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATACCTTTAAGAAATGGGGTTGGTCTGATGAAGATGTTATTCGAGTATTTAGGAGTCAACCTAATTTAATGTCTGTTTCAATTAATAAGATTGATTTGTTAATGAGTTTTTGGGTCAATCGATTGGGTTGGAATTCCTTGGCACTCACCAAGCGGCCACATATGTTTTCTTATAGTTTGGAGAAAAGGATCATCCCAAGGGACTCAGTTATGCAATTTCTTTTGATGAATGGTTTGCGAAAAAAAAATGCGAGCTTAGTTACACCATTTGCTTATTCTGAGAATATGTTCTTGAGCAAGTTTGTTTTCAGATTTATGGAGGAGTCTGATTATCTATTAAAGCTATATGAGGAAAAAATGAAACTTGCATACACAAAGAAGAACAATGGCATGCCATTCACTTAATATGTGAATTCATAATCTTCTTTTGTAATGTAGCCTTAGCTTGTTAGTTCTTAAAATTTAAGTGCTTATTCTTTCTAGGCCAAAATTTAAGAAGCTTTGGTGGGAGAGTTGCTAGAAGGTGGGAGTTGGGGTTTTATTTGGAGGCGTAGATTTTTTGTGTGGGAGGAGAACTTACTTAACAATTTATTGGAAGATTTGGATGGTTTTGTTCGGTCTCATGAGGAGGATAGATGGAGATGGTGATTGGAGGACGATGGTAGGTTCTCGGTTAAGTCGATGTATTTCAAGTTGGAAGGAGCTATGTTGGGGGAGTATAGGTGTCCGGTGGAGAAAATGAATACTTTTAAGCTTATTTGGAAGTCAGGGGCACCTTCAAAAGTGATGGCTTTTACTTGGAAGCTCCTACTTGACCGTATTCCTACTAGAGCAAATTTGGAGATTAGAAATTGCTTATCGCCGGAGGATGGATCAAATTGCATTTGGTGTACGACGGGGAGGGAATCTTCCACTCACCTTTTCCTTCATTGTGTTTTTGCCTATGATATATGGTTGTCTTTGATGAGGTGGACTAACCAGTTTTTCTTGATTCCTCCGAATTTGTTTATTCATTGGGAGTGTTGGAGTGCGGGAGGGTATCATAAGAAGATCCGGAGGGGTTGGAGAATGATTTGGCAAGCCGCTGTTTGGGTTATTTGGAAAAGACGTAATGACCGTATGTTCAATGATGTTGTTAAAGGGGTGGAGGAGTTGGTTGAAGAGATTCAGGTTTAATCTTGGCGGTGGATGATGGGAACGAGGGATACTCCGGTGTGTATGTTCTATGAGTGGAAGTGGTGTCTGGAGGCGTGTTTGCGGAGGTAAAGGGTGAGGGCAGGGACTGGTGGTCTGTGGTGTGTTATTTTCTGGTGCTGTTGCGTGTTTTCTGCTCACCTGCGTATCTGTTACTGTTGTTTTTCTGCTGCATTTTGGCTGCTGTTTTGTGCAGATAGCAGCACTGCCTGCTGCGTTTTTGTTTCCTCACGGGTGTGGCAGTGTTTGGGTTTTGTTATATTCCCCTTTGCTGTTATGTGTTGGCTTTTGGGGAGGAGTTATGGGATTGAATTCCGGTGTATTTAGTTAGATTTCAATGTCATATCTTTGCTTTTGGTGGGGTTTTGCTGTTATTGTATTTGGTGTTTTCGCGGTGTTTTGGTGGATGTTACGCTTATATGCGGCATCTTTGTTTTTTTTTTCACCACCTCTAAGCGTCTTGCTAGAGTTTCGGTTTCAATAATATTTGCCGATTAAAAAAAAAAAAAAAAAGAGGATAGTCCAGCTGACTTGTTTTTTTGCTAACTTGGACCAGAGTGCCAAAGTTGGTCATAGAAATATCTTATGATTTATCTTATGAAGTTATGCCTTATATCTAATAGATATAGAAAAGATTAAATGGTCTAGCATTTATTTGGTCAGGGAATGTTTTCTGTTAGTTCTCCTTGTATAAACAAACTATGTAGGCAGGTCTGATTTAAATTTTAAGCTTGAGATTATTAAGTACTTTGCCTAGCTACTTTTCCTTTGGTTCTTCACACACTATCATTTTAATCCATGTCACCTTGTTTTTGCTATTATCAATTTGAGGACTGATTTTGAAGTTTGCGATTCTACCATTGATAGATTGCTTCAAAAAAGGTCATCTATATTTGGTCAAGCTGGTTTGATTAAGTCATTGGAGGTTAACGGTTTAGGTTTTGATCCTTCAATGACTACTTTTGGGGCTTCCTTGATAGCCAAGAAATGTAGGAGTAAAACACGTTGGGATGAGAAGTTGATGTCTTTGAGAAACGGGGTTGGTCTGATGAAGCTATTTCTCAAGCATTTAGGTTGCAGTCTAGTTTGAGGTTGGCTTCAGTTATTTGGTGATGAGTTTTTGGGTCAATCTATTGGGTTGGAACTCCTTGGCACTTGCTAAAAGGCCGCATATTTTTGGTTTAAGTTTGCAGAAAAGGATCATTCCAAGGGTATTGGTTCTGCAATATCTTCTGTTGGAAGTTTGAAGAAAAAGATTGCAAGATTAGCTAAACCATTTACTTCTTTGATTTGAAGAACTTATTTGGTCTAGGACAGGATGGTGGTTACTGGTTAGTGTAGTTGTCTTGTTTCTCTAACTTGGACTACTGTGGGTTTGGTTTTTCTTATTATCAATTTCAGTTACAATTTTGATGCATAGGTCTTGCATCTAGTACGTTTTAATTTTAGTTACAATTTCTTCGTATCTAGTACATGTAAAAGTTCACATGGTCTTTCTTTTGGCCAGGGAATGCTTTTCTCATGGTGTTAGTTCTTCTTCCTTGTTAATTTAACCCAAGGGTCCTTTGTATATATACAAATTATATATGCAGGTTTATATATTTTCATGATTAAAGCTTTCAGTTTTAGATTGGTACCAGAGTGCATCTTTTAACCACTTTTCCCGTTTGTTCTCAACACTACATTGTCTATTAAATCTTTATGTGGAAAAAATTAAGGTTGTATACAACATGGAGAACACTGACGTTCCATTCCACCAAATAGGTGACTTCATAATCTTTTTTTTTTTGTAAGGTAATATTAGCTTGTTAGTTCTTAAATTTTAAGTGCTCATTCGTTTTGTAATGTAACATTAGCTTGTGACTTCATAATATGGGTTTAATTTTTGCGTTTCCAACTTCTTTTCATATTTTCCAATTTTTGTGCTCGTACTTAACATGTTATCTCTACTTCCGTTATTGTAGTATTTGTTAGCCATGGCAAGTGACAGAGACGAGTCTCTTCAATATATTGTACATTAAAAATTCCTGCTATCACTTGGAGGTTCTTTTGTTGGCCTCAATATAGTATGAATTGATAAAAACATTAACTTTGTTCGTCGATAAAAATTTATTTTTGGTGCTTTTGTTTCAGCTTTAGACATAGTAGTCAATAGCGGCGCTATAGCGGCGAATAGCGGCCGCGCGGTGCGGTGCGGTCCCGGTCCGCTATGCAATAAGCCATAGCGTAGCGTTATGAGATAGCGGCGCAATAGCGGCCGCAACACTGCATAGCGGTTTTGCAGTCTAGCACCGCTATTTCATTTTACGTGAAAATACAAGTTTGTCCTTGGTTTTTAAAAAAAAAACAAGGGTATTTTTGTCATTTTCGTTAAACCCTAATAACTTCAGTTTCTCTCTTCATTCTTTCATTCTTTACCGCCGCATCTTCTCATCTCAAAAACGCATCTCATCTTCTCACTGTTCATCTTCTCAAAACCGCATCAAACGGCAGATCTGTTCCTCTTCTCCTTACAGCCGCCGTTCCTCAGGTTTGCTCTCTTCTATAAATCTTCCTTTCTGTTTTGTTCATAAACATATCACAATATGTTTTGTTCTTCCTCTTTGTTTTGCCCTTCCTCTGTTTTGTTCATAAACATATCCTCTGTTTTGTTCTTCCTCTCTGTTTTCTCATGAATGATTGAAAAAAACACAAATGACTTCGGTTATCTGTGAGATCTCTATGATAAACTCTGCTCTCAGACGCAGGACACATTTAGTTCAATCTTTCTCTGTTGTTTTCCTCTACTGTTTTGTTCATCCTCTATTCTGCTCTCTGTTTCCTTATTTTTGTTGTTTTTGTGGACAACACAGTCATGGCGGCATCAGGTTCTGGTGTTCAATCAAACTCTGTGTCTGTTGACTCATGTGCTCAAAGACATAAGCGGACCGGTGATAATCTCGACATTGCTTGGCAATTTAATCACTGGAAGGATTTGAATGATAAGAAAAAGATTTACTGTGATTTCTGTGATAAGGTCAGCAATGGAGGAATTAGTAGAGCAAGAAGACACCAATTGGGAATTACGGGTGATGTAAAAGCATGTCGTAAAACACCAGCAGAGGTTAAGGCATTGTTGCAAGCTGATTTGGATGAGAAAAAAAAGAAATCAGAAGGTATTGCAGAGGTCCAAGCGCAAATAGATGATGATGTTCAAGAAGAAATTGAAGAAATAAGGAGAATTCGAACTGGAAAGAGGCCATCTGATTCCTCTTTGTCTGCTCCCAAAGCCAAGAAAGGTCCTTTGGATGTATTGTTTATGGGGGAAAAGACTACCAGTACCAGTACTAGGCAAACAAACATTAAAGATGCTGAAAAAGAAGCAAGGGCAAGAACCATACAATTTATTGCTCGATTCTTTTATACAAATGGAATTGCTTTTAATGTTGCACGGTCAAAACACTTCAAAGAAATGTTGGAAGCTGTTGGAGCTTATGGTCGTCATTTGAAACCACCAAGCTATTATGAAATTAGGGGACCACTACTTGAAATAGAGTTGGAAGAAACTAAGAAGATGTTGGTGCCTCATTTGGCCGCAAGGGTTAAATATGGATGCTCGATTATGTCCGATGGTTGGACAGACCGAAAAGGTAGAACCTTGATTAATTTTTTGGTGAATTCTACTGCTGGAACAATGTTTGTCAAAAGTATTGATGCTTCTGAAGAGTCAAAGACTGGAGAAATGGTGTATAAATTGATGAGTGATTTTGTTAATGAGATGGGTGAGCAAAATGTGGTGCAATTGATTACTGACAATGGAAGCAACTATGTTTATGCTGGCAAAAAGTTAATGCAGAACAAACCTCACATCTTTTGGACTCCATGTGCTGCACACTGCATAGACCTCATGTTGGAAGATATTGGTAAGATTCCTAGGATTAAAAAGGCTATACAAAAGGGGTGTTCTCTTGTAGGCTACATTTCTAACCACACATTTGCTTTGAGCCTAATGAGGAAGAAAACTAACAATGTTGAGTTGGTTAAACATGGAGTCACAAGGTTTGCTACCACATTTCTTACTTTGCAAAGGTTACACAAAATAAAATCCAAGCTTAGAGAGATGTTTTTATGTGAAGAATGGTTGAGTTCCAAGTGCAATAAGGAAGCTAAAGGTAGGCAGGCAGCAACATGTGCACTTCTGCCATCATTTTGGAATGATGTACTCCACACTCTTAAGTCCATGGCACCTCTTGTTGACGTGCTAAGAATGGTTGATAATGAGAAAAAACCTGCAATGGGTGAAATATATGCAGCCATGGATAATGCGAAGGAACAAATTGAAAAAAATTTCAATTTTAATGCAGGTCAGTATAAAGATGTGTTCAAAATCATTGATAAAAGATGGGAGTGCCAACTTCATCATCCATTGCATGCTACTGGTTACTACCTCAATCCTAAATACTTTTATAGCAAACCAGAAATTGAGAACGATCCCAAATTGCTGAGAGGTTTGCATAAATGCATTGAGACACTTTGTGAATGTAATGAAGTGGCAGACTTGGTTACAACTCAGTTGGGACAATTCAGGCGTGCTGGAGGATTATTTGGAGGAAAGGCAGCAGTAAGGCTACGGTCAAAACTGTCTCCAGGTAAATGGAAGTTAATTTCTTACAATTATATCACATAACTAGCAATTTTATTAAGAATTTAAGATCAAAACTGTGTATTTTTGCAGCTGAGTGGTGGATGAATTATGGAGCAGAAACTCCGGATTTGACACTAATGGCCATCAAAGTGCTGAGTTTGGTTTGTAGTGCTTCAGGATGTGAGCGCAACTGGTCTACTTTCGAGCATGTAAGCATTACACTAGCTTAAGTAACATTATTAGAGATTTAGAGTATCTTACAATCTTGGATATTAAACTAATTTTTAAATTTTACTATATAGATTCACTCCAAGAAGAGAAGTAAACTTGAGCATCAGAAGTTGCAAGAGTTGGTTTTCATTAAGTATAACCAAGCTTTGAAAGACCGATATGATTGTCTTGACAAGATTGACCCCGTGATGTTGTATGATTTTAATTATCATACTGAATGGTTAACAGGGGAGATGGGAGAGATGGGAGAGGATGGTGAACCTGTTGAAGAATTTGTTCATGAAAATGATGATCTAACTTGGGATCAAGTTGCCAATGCAAGTGGGGTTAATGAGCCTATTGTGAACACAAGGGCTAGTATTAGGGCAGAACGAGCAAAGGAACTTGCTGCAGCAGCAAGTTCAAATAGAGCATCAATTATACAAGAAGTATTTGTTGAAGATGTTGTAGGAATTGAAGAAGAAGATGATGATGATGATAATTGGTTGGAGGATGATCCGAATTATTATGGTGAAGAGGAGCTTTATGATGATGTTGATGAATGATGATGATCATCATTGATTCCAATGCTATGTTATGTTGGAATTATCAGTTCTTTTCTTTAGGATTTAA
Protein sequence

>MS.gene004646.t1

MFKAFLCNNAFRISTTTLYNAPFLFSLKFLTTTTTTSNPFIVSYLIDKFGFSHEFALKASKELYFKTSQKPDSVINFFKNHGFNDSDIRNIIKRVPGILSCNTHKTILPKFQFLLSKGASESDIVRMVKACRKTPAEVKALLQADLDEKKKKSEGIAEVQAQIDDDVQEEIEEIRRIRTGKRPSDSSLSAPKAKKGPLDVLFMGEKTTSTSTRQTNIKDAEKEARARTIQFIARFFYTNGIAFNVARSKHFKEMLEAVGAYGRHLKPPSYYEIRGPLLEIELEETKKMLVPHLAARVKYGCSIMSDGWTDRKGRTLINFLVNSTAGTMFVKSIDASEESKTGEMVYKLMSDFVNEMGEQNVVQLITDNGSNYVYAGKKLMQNKPHIFWTPCAAHCIDLMLEDIGKIPRIKKAIQKGCSLVGYISNHTFALSLMRKKTNNVELVKHGVTRFATTFLTLQRLHKIKSKLREMFLCEEWLSSKCNKEAKGRQAATCALLPSFWNDVLHTLKSMAPLVDVLRMVDNEKKPAMGEIYAAMDNAKEQIEKNFNFNAGQYKDVFKIIDKRWECQLHHPLHATGYYLNPKYFYSKPEIENDPKLLRGLHKCIETLCECNEVADLVTTQLGQFRRAGGLFGGKAAVRLRSKLSPAEWWMNYGAETPDLTLMAIKVLSLVCSASGCERNWSTFEHIHSKKRSKLEHQKLQELVFIKYNQALKDRYDCLDKIDPVMLYDFNYHTEWLTGEMGEMGEDGEPVEEFVHENDDLTWDQVANASGVNEPIVNTRASIRAERAKELAAAAILFFRI