AlfalfaGEDB | MS.gene004926

M. sativa cultivar XinJiangDaYe / MS.gene004926

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04770.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004926.t1	XP_013468809.1	92.1	191	11	1	8	194	1	191	7.60E-94	353.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004926.t1	Q9M9S0	49.0	198	84	4	10	190	84	281	1.6e-44	180.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004926.t1	G7L6E4	92.1	191	11	1	8	194	1	191	5.5e-94	353.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene004926.t1	TF	zf-HD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004926.t1	MTR_1g077020	92.147	191	11	1	8	194	1	191	4.18e-129	360
MS.gene004926.t1	MTR_4g134580	42.616	237	76	6	15	191	112	348	2.37e-55	179
MS.gene004926.t1	MTR_5g032600	44.670	197	87	4	14	189	76	271	6.04e-51	165
MS.gene004926.t1	MTR_3g100650	43.939	198	89	3	13	189	77	273	8.46e-50	162
MS.gene004926.t1	MTR_5g020510	40.394	203	90	5	16	191	99	297	1.89e-44	149
MS.gene004926.t1	MTR_5g065440	38.498	213	93	4	14	189	56	267	9.26e-43	144
MS.gene004926.t1	MTR_7g010300	39.706	204	90	6	13	189	85	282	5.06e-42	144
MS.gene004926.t1	MTR_6g012280	36.937	222	64	6	16	189	91	284	1.29e-38	135
MS.gene004926.t1	MTR_8g069080	36.893	206	85	4	15	185	68	263	1.99e-38	133
MS.gene004926.t1	MTR_2g076780	35.714	210	89	3	16	189	37	236	2.94e-38	132
MS.gene004926.t1	MTR_6g073013	35.465	172	79	4	16	185	19	160	2.68e-31	112
MS.gene004926.t1	MTR_8g467340	58.730	63	26	0	127	189	271	333	1.18e-19	85.5
MS.gene004926.t1	MTR_8g467340	64.407	59	17	2	12	67	60	117	3.10e-19	84.3
MS.gene004926.t1	MTR_3g039810	58.929	56	23	0	14	69	18	73	2.44e-19	79.0
MS.gene004926.t1	MTR_6g012220	60.000	55	18	1	10	64	15	65	7.11e-17	72.4
MS.gene004926.t1	MTR_8g069380	49.206	63	32	0	127	189	52	114	4.46e-15	68.6
MS.gene004926.t1	MTR_8g442330	30.872	149	71	4	25	164	14	139	5.47e-14	66.2
MS.gene004926.t1	MTR_8g051480	50.000	62	30	1	128	189	68	128	7.62e-13	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004926.t1	AT1G14440	48.990	198	84	4	10	190	84	281	1.83e-58	186
MS.gene004926.t1	AT1G14440	48.990	198	84	4	10	190	84	281	1.83e-58	186
MS.gene004926.t1	AT2G02540	48.293	205	82	4	9	189	80	284	2.42e-58	186
MS.gene004926.t1	AT2G02540	48.293	205	82	4	9	189	80	284	2.42e-58	186
MS.gene004926.t1	AT4G24660	46.927	179	86	2	14	189	47	219	1.23e-51	166
MS.gene004926.t1	AT4G24660	48.276	174	85	3	14	186	47	216	1.38e-50	163
MS.gene004926.t1	AT5G65410	41.848	184	92	2	15	188	74	252	3.55e-46	153
MS.gene004926.t1	AT2G18350	42.935	184	98	3	14	191	80	262	1.09e-45	152
MS.gene004926.t1	AT1G69600	41.414	198	92	6	8	186	23	215	1.44e-45	150
MS.gene004926.t1	AT5G60480	44.944	178	91	5	10	184	31	204	7.32e-45	148
MS.gene004926.t1	AT3G28920	40.952	210	79	5	14	186	50	251	6.27e-40	138
MS.gene004926.t1	AT3G50890	40.609	197	95	4	14	194	58	248	7.82e-40	136
MS.gene004926.t1	AT5G15210	42.424	198	80	7	9	183	49	235	7.96e-40	137
MS.gene004926.t1	AT5G42780	38.298	188	104	6	3	183	53	235	1.04e-26	102
MS.gene004926.t1	AT1G75240	68.966	58	18	0	11	68	71	128	3.52e-25	99.8
MS.gene004926.t1	AT1G75240	51.389	72	35	0	123	194	236	307	1.81e-17	79.0
MS.gene004926.t1	AT1G74660	61.404	57	22	0	14	70	37	93	1.64e-21	84.7
MS.gene004926.t1	AT1G74660	61.404	57	22	0	14	70	37	93	2.20e-21	84.7
MS.gene004926.t1	AT3G28917	62.069	58	21	1	14	70	31	88	3.58e-20	81.3
MS.gene004926.t1	AT1G18835	61.404	57	19	1	14	70	24	77	5.37e-19	78.2
MS.gene004926.t1	AT5G39760	53.030	66	31	0	127	192	200	265	8.72e-19	82.8
MS.gene004926.t1	AT5G39760	56.667	60	24	1	12	69	52	111	9.21e-16	74.3
MS.gene004926.t1	AT1G14687	30.769	182	88	7	16	183	7	164	1.21e-18	79.3

Find 24 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACATTAATGCACCTTTCT+TGG	0.224192	1.4:-63200108	MS.gene004926:CDS
CTTCTTAACTATCATCATTT+CGG	0.322468	1.4:+63199992	None:intergenic
TCATGAGAAAGATTGTATTA+AGG	0.341971	1.4:-63200030	MS.gene004926:CDS
GATGTCGTGCTTTGCAGAAA+AGG	0.406600	1.4:-63199931	MS.gene004926:CDS
GCTTGTAGAAAGATTTCTCT+TGG	0.457726	1.4:+63199794	None:intergenic
AAGAACCATGCAGCTACAAT+TGG	0.462343	1.4:-63200293	MS.gene004926:CDS
TATTATGCATCCACACTTTG+AGG	0.473414	1.4:+63199832	None:intergenic
ATTGGTGGCAATGCTATTGA+TGG	0.477828	1.4:-63200275	MS.gene004926:CDS
TTATCTCCTAACAACAAAAG+TGG	0.483711	1.4:-63200080	MS.gene004926:CDS
GGTGCATTAATGTTATGATC+AGG	0.492826	1.4:+63200116	None:intergenic
TAAGTTCAGTAAAGAACAGA+AGG	0.524622	1.4:-63199958	MS.gene004926:CDS
AACCATGCAGCTACAATTGG+TGG	0.533193	1.4:-63200290	MS.gene004926:CDS
GGAGATAAATGAGCCAAGAA+AGG	0.551764	1.4:+63200095	None:intergenic
TGCCACCAATTGTAGCTGCA+TGG	0.577086	1.4:+63200288	None:intergenic
GGCAATGCTATTGATGGATG+TGG	0.581028	1.4:-63200269	MS.gene004926:CDS
TCATGATAAGACTGATCAGA+AGG	0.590901	1.4:+63200047	None:intergenic
AAATGATGATAGTTAAGAAG+AGG	0.597202	1.4:-63199989	MS.gene004926:CDS
AGAGAAGAATCCTCAAAGTG+TGG	0.619701	1.4:-63199842	MS.gene004926:CDS
TAGCTTCTGAATCCTCCACT+CGG	0.628368	1.4:+63199908	None:intergenic
TTGCAGAAAAGGCCGAGTGG+AGG	0.631875	1.4:-63199920	MS.gene004926:CDS
GTGGAGGATTCAGAAGCTAG+AGG	0.637697	1.4:-63199904	MS.gene004926:CDS
TCAGAAGCTAGAGGAATCTG+TGG	0.646518	1.4:-63199895	MS.gene004926:CDS
TGTGGTGAGTTCATGCCAAG+TGG	0.691258	1.4:-63200251	MS.gene004926:CDS
GCTTTGCAGAAAAGGCCGAG+TGG	0.705464	1.4:-63199923	MS.gene004926:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATGATGATAGTTAAGAAG+AGG	-	chr1.4:63200155-63200174	MS.gene004926:CDS	25.0%
!	CTTCTTAACTATCATCATTT+CGG	+	chr1.4:63200155-63200174	None:intergenic	25.0%
!	TCATGAGAAAGATTGTATTA+AGG	-	chr1.4:63200114-63200133	MS.gene004926:CDS	25.0%
	TAACATTAATGCACCTTTCT+TGG	-	chr1.4:63200036-63200055	MS.gene004926:CDS	30.0%
	TAAGTTCAGTAAAGAACAGA+AGG	-	chr1.4:63200186-63200205	MS.gene004926:CDS	30.0%
	TTATCTCCTAACAACAAAAG+TGG	-	chr1.4:63200064-63200083	MS.gene004926:CDS	30.0%
	GCTTGTAGAAAGATTTCTCT+TGG	+	chr1.4:63200353-63200372	None:intergenic	35.0%
	GGTGCATTAATGTTATGATC+AGG	+	chr1.4:63200031-63200050	None:intergenic	35.0%
	TATTATGCATCCACACTTTG+AGG	+	chr1.4:63200315-63200334	None:intergenic	35.0%
	TCATGATAAGACTGATCAGA+AGG	+	chr1.4:63200100-63200119	None:intergenic	35.0%
!	CTTTTCTGTGGAAGTTTCTA+TGG	+	chr1.4:63199964-63199983	None:intergenic	35.0%
!!	CACAAGTTTTGTCAAGAGAT+TGG	-	chr1.4:63200274-63200293	MS.gene004926:CDS	35.0%
!!	CAGACTCTATTTCTTTTCTG+TGG	+	chr1.4:63199976-63199995	None:intergenic	35.0%
!!!	GTTGATCCACTTTTGTTGTT+AGG	+	chr1.4:63200073-63200092	None:intergenic	35.0%
	AAGAACCATGCAGCTACAAT+TGG	-	chr1.4:63199851-63199870	MS.gene004926:CDS	40.0%
	AGAGAAGAATCCTCAAAGTG+TGG	-	chr1.4:63200302-63200321	MS.gene004926:CDS	40.0%
	GGAGATAAATGAGCCAAGAA+AGG	+	chr1.4:63200052-63200071	None:intergenic	40.0%
!	ATTGGTGGCAATGCTATTGA+TGG	-	chr1.4:63199869-63199888	MS.gene004926:CDS	40.0%
!!!	AGTGTGTCATTTTCTCCACT+TGG	+	chr1.4:63199911-63199930	None:intergenic	40.0%
	AACCATGCAGCTACAATTGG+TGG	-	chr1.4:63199854-63199873	MS.gene004926:CDS	45.0%
	GATGTCGTGCTTTGCAGAAA+AGG	-	chr1.4:63200213-63200232	MS.gene004926:CDS	45.0%
	TAGCTTCTGAATCCTCCACT+CGG	+	chr1.4:63200239-63200258	None:intergenic	45.0%
	TCAGAAGCTAGAGGAATCTG+TGG	-	chr1.4:63200249-63200268	MS.gene004926:CDS	45.0%
!	GGCAATGCTATTGATGGATG+TGG	-	chr1.4:63199875-63199894	MS.gene004926:CDS	45.0%
	GTGGAGGATTCAGAAGCTAG+AGG	-	chr1.4:63200240-63200259	MS.gene004926:CDS	50.0%
	TGCCACCAATTGTAGCTGCA+TGG	+	chr1.4:63199859-63199878	None:intergenic	50.0%
	TGTGGTGAGTTCATGCCAAG+TGG	-	chr1.4:63199893-63199912	MS.gene004926:CDS	50.0%
	GCTTTGCAGAAAAGGCCGAG+TGG	-	chr1.4:63200221-63200240	MS.gene004926:CDS	55.0%
	TTGCAGAAAAGGCCGAGTGG+AGG	-	chr1.4:63200224-63200243	MS.gene004926:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	63199791	63200375	63199791	ID=MS.gene004926
chr1.4	mRNA	63199791	63200375	63199791	ID=MS.gene004926.t1;Parent=MS.gene004926
chr1.4	exon	63199791	63200375	63199791	ID=MS.gene004926.t1.exon1;Parent=MS.gene004926.t1
chr1.4	CDS	63199791	63200375	63199791	ID=cds.MS.gene004926.t1;Parent=MS.gene004926.t1

Gene Sequence

>MS.gene004926

ATGGAGTCTAGCATTTTTCATGTTGAGAAAAAGATAGTAGTAAAATATAAAGAGTGTCTCAAGAACCATGCAGCTACAATTGGTGGCAATGCTATTGATGGATGTGGTGAGTTCATGCCAAGTGGAGAAAATGACACACTTGAAGCTCTCAAGTGTTGTGCTTGCAATTGCCATAGAAACTTCCACAGAAAAGAAATAGAGTCTGATTTTCAACATTATGCTAATCTTTCACTGATTCCTGATCATAACATTAATGCACCTTTCTTGGCTCATTTATCTCCTAACAACAAAAGTGGATCAACTAGTCCTTCTGATCAGTCTTATCATGAGAAAGATTGTATTAAGGATGTCGAAAATCGAACCGAAATGATGATAGTTAAGAAGAGGTCTAGAACTAAGTTCAGTAAAGAACAGAAGGAGAAGATGTCGTGCTTTGCAGAAAAGGCCGAGTGGAGGATTCAGAAGCTAGAGGAATCTGTGGTGCACAAGTTTTGTCAAGAGATTGGAATCAAGAGAAGAATCCTCAAAGTGTGGATGCATAATAACAAGCACTCTTTTGCCAAGAGAAATCTTTCTACAAGCTAG

Protein sequence

>MS.gene004926.t1

MESSIFHVEKKIVVKYKECLKNHAATIGGNAIDGCGEFMPSGENDTLEALKCCACNCHRNFHRKEIESDFQHYANLSLIPDHNINAPFLAHLSPNNKSGSTSPSDQSYHEKDCIKDVENRTEMMIVKKRSRTKFSKEQKEKMSCFAEKAEWRIQKLEESVVHKFCQEIGIKRRILKVWMHNNKHSFAKRNLSTS