AlfalfaGEDB | MS.gene004965

M. sativa cultivar XinJiangDaYe / MS.gene004965

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004965.t1	RHN80500.1	69.5	82	20	1	1	77	1	82	2.40E-22	114.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004965.t1	Q9ZQ70	57.3	82	29	1	2	77	354	435	1.4e-18	93.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004965.t1	A0A072VLP6	69.5	82	20	1	1	77	1	82	1.7e-22	114.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene004965.t1	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004965.t1	MTR_1g077780	69.512	82	20	1	1	77	1	82	3.05e-32	109
MS.gene004965.t1	MTR_1g077760	83.333	54	9	0	24	77	81	134	1.66e-27	96.7
MS.gene004965.t1	MTR_5g042920	58.571	70	29	0	8	77	271	340	3.01e-21	85.5
MS.gene004965.t1	MTR_3g009470	55.072	69	29	1	9	77	449	515	1.09e-17	75.9
MS.gene004965.t1	MTR_3g009470	55.072	69	29	1	9	77	449	515	1.21e-17	75.5
MS.gene004965.t1	MTR_4g082580	53.947	76	32	2	4	77	224	298	3.11e-16	71.6
MS.gene004965.t1	MTR_7g011820	50.000	74	33	2	4	77	361	430	5.53e-16	70.9
MS.gene004965.t1	MTR_4g013260	50.000	66	33	0	12	77	291	356	2.57e-15	68.9
MS.gene004965.t1	MTR_5g074400	72.500	40	11	0	38	77	401	440	6.26e-15	67.8
MS.gene004965.t1	MTR_3g031220	72.500	40	11	0	38	77	346	385	8.66e-15	67.4
MS.gene004965.t1	MTR_3g031220	72.500	40	11	0	38	77	346	385	8.99e-15	67.4
MS.gene004965.t1	MTR_7g071120	64.444	45	16	0	33	77	300	344	9.24e-15	67.4
MS.gene004965.t1	MTR_7g080020	70.000	40	12	0	38	77	474	513	4.63e-14	65.5
MS.gene004965.t1	MTR_5g094430	72.973	37	10	0	41	77	348	384	5.23e-14	65.5
MS.gene004965.t1	MTR_7g080000	72.500	40	11	0	38	77	413	452	6.18e-14	65.1
MS.gene004965.t1	MTR_5g094430	72.973	37	10	0	41	77	236	272	6.22e-14	65.1
MS.gene004965.t1	MTR_5g094430	72.973	37	10	0	41	77	214	250	7.04e-14	64.7
MS.gene004965.t1	MTR_3g056100	70.270	37	11	0	41	77	399	435	9.25e-14	64.7
MS.gene004965.t1	MTR_3g056100	70.270	37	11	0	41	77	386	422	9.51e-14	64.7
MS.gene004965.t1	MTR_3g056100	70.270	37	11	0	41	77	242	278	1.34e-13	63.9
MS.gene004965.t1	MTR_3g056100	70.270	37	11	0	41	77	255	291	1.34e-13	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene004965.t1	AT2G03340	57.317	82	29	1	2	77	354	435	2.47e-21	86.3
MS.gene004965.t1	AT1G13960	53.012	83	32	2	2	77	194	276	2.37e-19	80.1
MS.gene004965.t1	AT1G13960	53.012	83	32	2	2	77	347	429	3.03e-19	80.1
MS.gene004965.t1	AT4G12020	53.659	82	32	1	2	77	580	661	8.28e-19	79.0
MS.gene004965.t1	AT4G12020	53.659	82	32	1	2	77	580	661	8.28e-19	79.0
MS.gene004965.t1	AT4G12020	53.659	82	32	1	2	77	580	661	8.28e-19	79.0
MS.gene004965.t1	AT4G12020	53.659	82	32	1	2	77	580	661	8.28e-19	79.0
MS.gene004965.t1	AT4G12020	53.659	82	32	1	2	77	580	661	8.28e-19	79.0
MS.gene004965.t1	AT4G12020	53.659	82	32	1	2	77	580	661	9.52e-19	79.0
MS.gene004965.t1	AT4G12020	53.659	82	32	1	2	77	580	661	1.04e-18	78.6
MS.gene004965.t1	AT4G12020	53.659	82	32	1	2	77	580	661	1.04e-18	78.6
MS.gene004965.t1	AT4G12020	62.295	61	23	0	17	77	534	594	8.58e-18	76.3
MS.gene004965.t1	AT4G12020	62.295	61	23	0	17	77	601	661	9.19e-18	75.9
MS.gene004965.t1	AT4G12020	62.295	61	23	0	17	77	601	661	9.19e-18	75.9
MS.gene004965.t1	AT4G26440	54.839	62	28	0	16	77	331	392	4.53e-17	73.9
MS.gene004965.t1	AT5G56270	58.929	56	22	1	22	77	453	507	4.61e-16	71.2
MS.gene004965.t1	AT2G38470	48.485	66	34	0	12	77	317	382	5.46e-15	68.2
MS.gene004965.t1	AT5G07100	72.973	37	10	0	41	77	125	161	2.09e-14	65.1
MS.gene004965.t1	AT5G07100	72.973	37	10	0	41	77	125	161	2.09e-14	65.1
MS.gene004965.t1	AT5G07100	72.973	37	10	0	41	77	125	161	2.09e-14	65.1
MS.gene004965.t1	AT5G07100	72.973	37	10	0	41	77	125	161	2.09e-14	65.1
MS.gene004965.t1	AT5G07100	72.973	37	10	0	41	77	218	254	3.82e-14	65.5
MS.gene004965.t1	AT4G26640	70.270	37	11	0	41	77	365	401	1.57e-13	63.9
MS.gene004965.t1	AT4G26640	70.270	37	11	0	41	77	325	361	1.71e-13	63.9
MS.gene004965.t1	AT4G26640	70.270	37	11	0	41	77	293	329	1.74e-13	63.9
MS.gene004965.t1	AT4G26640	70.270	37	11	0	41	77	189	225	2.40e-13	63.5
MS.gene004965.t1	AT4G26640	70.270	37	11	0	41	77	189	225	2.40e-13	63.5
MS.gene004965.t1	AT4G26640	70.270	37	11	0	41	77	189	225	2.40e-13	63.5
MS.gene004965.t1	AT4G26640	70.270	37	11	0	41	77	189	225	2.40e-13	63.5
MS.gene004965.t1	AT3G01080	50.943	53	26	0	25	77	274	326	3.39e-13	63.2
MS.gene004965.t1	AT3G01080	50.943	53	26	0	25	77	312	364	3.39e-13	63.2
MS.gene004965.t1	AT2G30250	64.865	37	13	0	41	77	312	348	8.33e-11	56.2

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAATATCACCTAGGATT+AGG	0.273726	1.4:+63700298	None:intergenic
GTTGTTATTATGATATTTCT+AGG	0.305063	1.4:+63700391	None:intergenic
ATCAAATAGCTTCTTCATAT+AGG	0.310605	1.4:-63700426	MS.gene004965:CDS
TCAAATAGCTTCTTCATATA+GGG	0.328504	1.4:-63700425	MS.gene004965:CDS
TCTTAGACGATGGATATAAT+TGG	0.350156	1.4:-63700354	MS.gene004965:CDS
GAACTAGGTTGTGATGAAAT+TGG	0.383740	1.4:-63700581	MS.gene004965:CDS
TATGATATTTCTAGGCTCTC+TGG	0.387148	1.4:+63700399	None:intergenic
AGAAATATCACCTAGGATTA+GGG	0.390952	1.4:+63700299	None:intergenic
GGATATAATTGGAGAAAATA+CGG	0.397885	1.4:-63700343	MS.gene004965:CDS
CCTAGTTCTGCATTGTCGCT+TGG	0.455776	1.4:+63700596	None:intergenic
GAGTTGAACCTGGCCCCAAT+AGG	0.469252	1.4:-63700559	MS.gene004965:CDS
GAGATTGATGTCTTAGACGA+TGG	0.470244	1.4:-63700364	MS.gene004965:CDS
AGAAAATACGGGCAAAGAGT+TGG	0.502503	1.4:-63700331	MS.gene004965:CDS
ACCTACCTCCTATTGGGGCC+AGG	0.505111	1.4:+63700551	None:intergenic
CCAAGCGACAATGCAGAACT+AGG	0.516569	1.4:-63700596	MS.gene004965:CDS
ATGATATTTCTAGGCTCTCT+GGG	0.523221	1.4:+63700400	None:intergenic
GCAAAGGAAACCCTAATCCT+AGG	0.526000	1.4:-63700309	MS.gene004965:CDS
GATATAATTGGAGAAAATAC+GGG	0.530666	1.4:-63700342	MS.gene004965:CDS
GTCTAAGACATCAATCTCAC+TGG	0.554144	1.4:+63700369	None:intergenic
GATGAAATTGGAGTTGAACC+TGG	0.567212	1.4:-63700569	MS.gene004965:CDS
TTGAACCTGGCCCCAATAGG+AGG	0.579170	1.4:-63700556	MS.gene004965:intron
TTGCAGAAATACAGAGATCA+CGG	0.600046	1.4:-63700455	MS.gene004965:intron
TACGGGCAAAGAGTTGGCAA+AGG	0.637540	1.4:-63700325	MS.gene004965:CDS
AAGGTGAAGAAATATCACCT+AGG	0.710430	1.4:+63700292	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTAAACACAAATATGTTTA+TGG	-	chr1.4:63700386-63700405	MS.gene004965:CDS	20.0%
!!!	GTTGTTATTATGATATTTCT+AGG	+	chr1.4:63700520-63700539	None:intergenic	20.0%
!	AAACACAAATATGTTTATGG+TGG	-	chr1.4:63700389-63700408	MS.gene004965:CDS	25.0%
!	ATCAAATAGCTTCTTCATAT+AGG	-	chr1.4:63700482-63700501	MS.gene004965:intron	25.0%
!	GATATAATTGGAGAAAATAC+GGG	-	chr1.4:63700566-63700585	MS.gene004965:CDS	25.0%
!	GGATATAATTGGAGAAAATA+CGG	-	chr1.4:63700565-63700584	MS.gene004965:CDS	25.0%
!	TCAAATAGCTTCTTCATATA+GGG	-	chr1.4:63700483-63700502	MS.gene004965:intron	25.0%
	AATAAAAACCTACCTCCTAT+TGG	+	chr1.4:63700367-63700386	None:intergenic	30.0%
	ATAAAAACCTACCTCCTATT+GGG	+	chr1.4:63700366-63700385	None:intergenic	30.0%
	TCTTAGACGATGGATATAAT+TGG	-	chr1.4:63700554-63700573	MS.gene004965:intron	30.0%
	GAACTAGGTTGTGATGAAAT+TGG	-	chr1.4:63700327-63700346	MS.gene004965:CDS	35.0%
	TAAAAACCTACCTCCTATTG+GGG	+	chr1.4:63700365-63700384	None:intergenic	35.0%
	TTGCAGAAATACAGAGATCA+CGG	-	chr1.4:63700453-63700472	MS.gene004965:intron	35.0%
!!	ATGATATTTCTAGGCTCTCT+GGG	+	chr1.4:63700511-63700530	None:intergenic	35.0%
!!	TATGATATTTCTAGGCTCTC+TGG	+	chr1.4:63700512-63700531	None:intergenic	35.0%
	AGAAAATACGGGCAAAGAGT+TGG	-	chr1.4:63700577-63700596	MS.gene004965:CDS	40.0%
	GAGATTGATGTCTTAGACGA+TGG	-	chr1.4:63700544-63700563	MS.gene004965:intron	40.0%
	GATGAAATTGGAGTTGAACC+TGG	-	chr1.4:63700339-63700358	MS.gene004965:CDS	40.0%
!	GTCTAAGACATCAATCTCAC+TGG	+	chr1.4:63700542-63700561	None:intergenic	40.0%
	GCAAAGGAAACCCTAATCCT+AGG	-	chr1.4:63700599-63700618	MS.gene004965:CDS	45.0%
	CCAAGCGACAATGCAGAACT+AGG	-	chr1.4:63700312-63700331	MS.gene004965:CDS	50.0%
	TACGGGCAAAGAGTTGGCAA+AGG	-	chr1.4:63700583-63700602	MS.gene004965:CDS	50.0%
!!	CCTAGTTCTGCATTGTCGCT+TGG	+	chr1.4:63700315-63700334	None:intergenic	50.0%
	GAGTTGAACCTGGCCCCAAT+AGG	-	chr1.4:63700349-63700368	MS.gene004965:CDS	55.0%
	TTGAACCTGGCCCCAATAGG+AGG	-	chr1.4:63700352-63700371	MS.gene004965:CDS	55.0%
	ACCTACCTCCTATTGGGGCC+AGG	+	chr1.4:63700360-63700379	None:intergenic	60.0%
	ACCTGGCCCCAATAGGAGGT+AGG	-	chr1.4:63700356-63700375	MS.gene004965:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	63700306	63700624	63700306	ID=MS.gene004965
chr1.4	mRNA	63700306	63700624	63700306	ID=MS.gene004965.t1;Parent=MS.gene004965
chr1.4	exon	63700557	63700624	63700557	ID=MS.gene004965.t1.exon1;Parent=MS.gene004965.t1
chr1.4	CDS	63700557	63700624	63700557	ID=cds.MS.gene004965.t1;Parent=MS.gene004965.t1
chr1.4	exon	63700306	63700471	63700306	ID=MS.gene004965.t1.exon2;Parent=MS.gene004965.t1
chr1.4	CDS	63700306	63700471	63700306	ID=cds.MS.gene004965.t1;Parent=MS.gene004965.t1

Gene Sequence	>MS.gene004965 ATGGAACCAAGCGACAATGCAGAACTAGGTTGTGATGAAATTGGAGTTGAACCTGGCCCCAATAGGAGGTAGGTTTTTATTGTAAACACAAATATGTTTATGGTGGATAATCATTTTCTTTTATGTAATCTTGCTATTGTTGCGATTTTGCAGAAATACAGAGATCACGGTCTTAAATCAAATAGCTTCTTCATATAGGGCTCCCAGAGAGCCTAGAAATATCATAATAACAACCAGTGAGATTGATGTCTTAGACGATGGATATAATTGGAGAAAATACGGGCAAAGAGTTGGCAAAGGAAACCCTAATCCTAGGTGA
Protein sequence	>MS.gene004965.t1 MEPSDNAELGCDEIGVEPGPNRRNTEITVLNQIASSYRAPREPRNIIITTSEIDVLDDGYNWRKYGQRVGKGNPNPR