AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005143

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005143.t1 MTR_1g081180 95.407 479 16 4 1 478 84 557 0.0 927
MS.gene005143.t1 MTR_7g114860 46.485 441 154 16 3 428 106 479 9.71e-105 321
MS.gene005143.t1 MTR_1g098900 35.374 294 159 7 146 426 266 541 1.24e-47 174
MS.gene005143.t1 MTR_1g098900 40.000 100 57 2 326 425 46 142 3.30e-16 81.6
MS.gene005143.t1 MTR_1g098900 43.529 85 43 1 21 100 58 142 2.42e-14 75.9
MS.gene005143.t1 MTR_2g026725 34.043 282 154 6 174 449 117 372 3.77e-37 140
MS.gene005143.t1 MTR_1g098870 34.363 259 136 4 171 428 236 461 3.74e-35 138
MS.gene005143.t1 MTR_1g098870 45.882 85 41 1 21 100 56 140 8.16e-15 77.4
MS.gene005143.t1 MTR_1g098870 40.217 92 52 2 337 428 55 143 3.61e-14 75.1
MS.gene005143.t1 MTR_6g035370 59.783 92 35 1 337 428 464 553 1.11e-27 117
MS.gene005143.t1 MTR_6g035370 40.860 93 52 2 335 427 53 142 1.79e-15 79.3
MS.gene005143.t1 MTR_6g035370 42.353 85 44 1 21 100 56 140 3.94e-14 75.1
MS.gene005143.t1 MTR_1g060990 29.289 239 150 7 21 244 132 366 1.80e-18 88.6
MS.gene005143.t1 MTR_1g060990 39.785 93 52 3 337 428 131 220 1.09e-12 70.5
MS.gene005143.t1 MTR_1g492790 36.090 133 72 4 300 422 68 197 2.44e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005143.t1 AT5G28300 45.188 478 172 15 20 428 102 558 7.81e-102 318
MS.gene005143.t1 AT1G76890 38.806 268 130 4 162 428 252 486 2.32e-47 172
MS.gene005143.t1 AT1G76890 46.591 88 44 2 335 422 39 123 1.56e-16 82.8
MS.gene005143.t1 AT1G76890 45.783 83 40 1 21 98 42 124 3.74e-14 75.1
MS.gene005143.t1 AT1G76880 58.696 92 36 1 337 428 408 497 2.55e-28 118
MS.gene005143.t1 AT1G76880 39.048 105 61 2 335 439 59 160 3.26e-15 78.6
MS.gene005143.t1 AT1G76880 44.706 85 42 1 21 100 62 146 1.08e-12 70.9
MS.gene005143.t1 AT1G33240 51.786 112 43 2 337 439 435 544 9.67e-27 114
MS.gene005143.t1 AT1G33240 43.820 89 47 2 337 425 62 147 3.67e-17 84.7
MS.gene005143.t1 AT1G33240 45.882 85 41 1 21 100 63 147 1.62e-16 82.8
MS.gene005143.t1 AT1G33240 51.786 112 43 2 337 439 435 544 1.01e-26 114
MS.gene005143.t1 AT1G33240 43.820 89 47 2 337 425 62 147 3.59e-17 84.7
MS.gene005143.t1 AT1G33240 45.882 85 41 1 21 100 63 147 2.12e-16 82.4
MS.gene005143.t1 AT1G33240 51.786 112 43 2 337 439 396 505 1.04e-26 114
MS.gene005143.t1 AT5G47660 33.209 268 131 12 159 426 169 388 3.82e-19 89.7
MS.gene005143.t1 AT3G10000 27.841 176 113 5 257 430 17 180 1.30e-14 76.3
MS.gene005143.t1 AT5G03680 42.857 91 48 3 337 426 119 206 1.10e-13 73.9
MS.gene005143.t1 AT3G10000 25.714 175 108 5 257 430 17 170 9.49e-12 67.4

Find 86 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAGATTTGAAGTGTTTA+AGG 0.195477 1.1:-60327404 MS.gene005143:CDS
GTCTTTGATCTCTTTCAATT+TGG 0.216907 1.1:+60328308 None:intergenic
ATAAGGCTATATGAAGAAAT+AGG 0.236435 1.1:+60326944 None:intergenic
CTCTCTCGCTGCTCGATATA+AGG 0.299308 1.1:+60326927 None:intergenic
TCCTTCTTCTTCTACTACTT+TGG 0.306363 1.1:-60326990 MS.gene005143:CDS
AATGCCAACACTTCATCTTT+TGG 0.317647 1.1:+60326884 None:intergenic
AATCATAATCAAAATAGTTT+TGG 0.326250 1.1:-60327658 MS.gene005143:CDS
AGGGTTGAGCTAGGGTTTAC+AGG 0.337022 1.1:+60327013 None:intergenic
TTTCAAGAGTTTCCGGATTA+GGG 0.337138 1.1:+60327053 None:intergenic
TCACAAGGAATGTTGGAGTT+AGG 0.345818 1.1:-60326782 MS.gene005143:CDS
ACATGTTCCCAAGTGAAATC+TGG 0.352061 1.1:+60328199 None:intergenic
GAACGAGGTGCAAGACAAAA+TGG 0.355649 1.1:-60327545 MS.gene005143:CDS
GAACAAAGAGCTTGAGTTGA+TGG 0.361917 1.1:-60327242 MS.gene005143:CDS
GAGCTAGGGTTTACAGGTAT+TGG 0.369227 1.1:+60327019 None:intergenic
GATAGATTTGAAGTGTTTAA+GGG 0.370463 1.1:-60327403 MS.gene005143:CDS
AGCAGCGAGAGAGACGATAT+TGG 0.377007 1.1:-60326917 MS.gene005143:CDS
GAAGAAGGAAGGGTTGAGCT+AGG 0.386310 1.1:+60327004 None:intergenic
CAACAACAAGGCACCACTTT+GGG 0.393210 1.1:-60326813 MS.gene005143:CDS
TTCTTCTTCTACTACTTTGG+TGG 0.410965 1.1:-60326987 MS.gene005143:CDS
AGTTTAGTAGAGAAGAAGCT+TGG 0.425700 1.1:-60327288 MS.gene005143:CDS
TTGAGCAATCTTTATAATCA+AGG 0.426028 1.1:-60326635 MS.gene005143:CDS
GAGAAGAAGAGGAAAAGATC+AGG 0.430204 1.1:-60327430 MS.gene005143:CDS
TAAGGCTATATGAAGAAATA+GGG 0.437015 1.1:+60326945 None:intergenic
GAGAATTTCACAAGGAATGT+TGG 0.441400 1.1:-60326789 MS.gene005143:CDS
TTCCTCAACTCATTGATCCT+TGG 0.453786 1.1:-60328279 MS.gene005143:CDS
CAACAACAACAATGAAGAAA+AGG 0.456418 1.1:-60326846 MS.gene005143:CDS
CACCTGCACCAGCACCATCT+TGG 0.457236 1.1:+60326516 None:intergenic
AAATCTAGTTGAGGCTGAAA+AGG 0.460400 1.1:-60327569 MS.gene005143:CDS
AAGAAGGAAGGGTTGAGCTA+GGG 0.460441 1.1:+60327005 None:intergenic
TTGCTCAATAGATGAAAGTA+AGG 0.463276 1.1:+60326650 None:intergenic
CTCAAGTGGGGCCTCCAAGC+TGG 0.476139 1.1:-60326487 MS.gene005143:CDS
GAGGTTTATCAAGGTGGTGG+GGG 0.479843 1.1:-60327604 MS.gene005143:CDS
TTCAAGAATGGATGATGTAT+TGG 0.485834 1.1:-60327503 MS.gene005143:CDS
CAAAGGATGCTAATAGGAAA+AGG 0.488346 1.1:-60326694 MS.gene005143:CDS
GCAACAACAAGGCACCACTT+TGG 0.490808 1.1:-60326814 MS.gene005143:CDS
GTCAAAGAAAAGTGAAGAAG+AGG 0.493141 1.1:-60327479 MS.gene005143:CDS
TGAAGATGACATTGAAGCTA+AGG 0.499602 1.1:+60327135 None:intergenic
GAAGAAGAGGTAGTTGAGAA+AGG 0.502624 1.1:-60327466 MS.gene005143:CDS
GCACATGAACAAGCTATTGC+TGG 0.507249 1.1:-60327220 MS.gene005143:CDS
CATGTTCCCAAGTGAAATCT+GGG 0.514235 1.1:+60328200 None:intergenic
GTTGGTTCCCAGATTTCACT+TGG 0.516402 1.1:-60328207 MS.gene005143:CDS
GCACTCACAAAACCCTAATC+CGG 0.519084 1.1:-60327065 MS.gene005143:CDS
GAAGAGGTTTATCAAGGTGG+TGG 0.520513 1.1:-60327607 MS.gene005143:CDS
AACCAAGATGGTGCTGGTGC+AGG 0.525566 1.1:-60326518 MS.gene005143:CDS
GTTTGTTACTGAGCTGGAAG+AGG 0.529219 1.1:-60327623 MS.gene005143:CDS
AAGTAGTAGAAGAAGAAGGA+AGG 0.534716 1.1:+60326993 None:intergenic
GAGACGATATTGGTAGAAGA+TGG 0.544390 1.1:-60326907 MS.gene005143:CDS
TGGGAACCAACTCTCCATGC+TGG 0.546340 1.1:+60328219 None:intergenic
CAAAGAGGTGCAAAGAGAAG+TGG 0.549838 1.1:-60326745 MS.gene005143:CDS
TGTAGAGGAGGATTCAAGAA+TGG 0.554166 1.1:-60327515 MS.gene005143:CDS
AGAGGTTTATCAAGGTGGTG+GGG 0.556530 1.1:-60327605 MS.gene005143:CDS
TAGTGAAATTACCAGCTTGG+AGG 0.560803 1.1:+60326476 None:intergenic
TCAGATCCAGCATGGAGAGT+TGG 0.563903 1.1:-60328225 MS.gene005143:CDS
CTACAAATGATGAGAAGAAG+AGG 0.565637 1.1:-60327441 MS.gene005143:CDS
ACCAAAGTAGTAGAAGAAGA+AGG 0.573800 1.1:+60326989 None:intergenic
ATGGCCAAAAGATGAAGTGT+TGG 0.578682 1.1:-60326888 MS.gene005143:CDS
AAGAGGTTTATCAAGGTGGT+GGG 0.581021 1.1:-60327606 MS.gene005143:CDS
CTTGAATCCTCCTCTACATG+AGG 0.583768 1.1:+60327520 None:intergenic
AAAGAGGTGCAAAGAGAAGT+GGG 0.586402 1.1:-60326744 MS.gene005143:CDS
GAGCTGGAAGAGGTTTATCA+AGG 0.586678 1.1:-60327613 MS.gene005143:CDS
ATATAGTGAAATTACCAGCT+TGG 0.588322 1.1:+60326473 None:intergenic
AGTAGTAGAAGAAGAAGGAA+GGG 0.592188 1.1:+60326994 None:intergenic
GAGTGTGGTGAAGAAAATGA+TGG 0.595280 1.1:-60327371 MS.gene005143:CDS
GTCCAAGGATCAATGAGTTG+AGG 0.597929 1.1:+60328277 None:intergenic
GAAATAGGGTTGTTCTTGCG+AGG 0.598239 1.1:+60326959 None:intergenic
TGTGAAATTCTCTCCCAAAG+TGG 0.599247 1.1:+60326800 None:intergenic
AGCTTGGAAGAAACAAGAGA+TGG 0.599559 1.1:-60327272 MS.gene005143:CDS
TTTGAAGATCAGATCCAGCA+TGG 0.607084 1.1:-60328233 MS.gene005143:CDS
CTTTGGGAGAGAATTTCACA+AGG 0.610777 1.1:-60326797 MS.gene005143:CDS
CAAAATGGACCCTCATGTAG+AGG 0.618548 1.1:-60327530 MS.gene005143:CDS
GATGAAAACCAAGATGGTGC+TGG 0.618902 1.1:-60326524 MS.gene005143:CDS
TCACTTGGGAACATGTCTCA+AGG 0.620742 1.1:-60328192 MS.gene005143:intron
AACAACAATGAAGAAAAGGA+AGG 0.621045 1.1:-60326842 MS.gene005143:CDS
AGAACTTCATCATTAGTCCA+AGG 0.625382 1.1:+60328262 None:intergenic
AGAAAGCAATAACGTGAATG+TGG 0.625602 1.1:-60326582 MS.gene005143:CDS
TTGAATCCTCCTCTACATGA+GGG 0.632417 1.1:+60327521 None:intergenic
TTGGTTCCCAGATTTCACTT+GGG 0.635745 1.1:-60328206 MS.gene005143:CDS
CACAGGAAGCTAGCTGACGT+TGG 0.645573 1.1:-60327754 MS.gene005143:intron
ACACAAGATGAAAACCAAGA+TGG 0.647031 1.1:-60326530 MS.gene005143:CDS
AATGGACCCTCATGTAGAGG+AGG 0.651973 1.1:-60327527 MS.gene005143:CDS
CTGGAAGAGGTTTATCAAGG+TGG 0.657958 1.1:-60327610 MS.gene005143:CDS
TGAGGCTGAAAAGGCGAACG+AGG 0.666720 1.1:-60327560 MS.gene005143:CDS
TGTTGGCATTGATAAACTTG+AGG 0.669533 1.1:-60326871 MS.gene005143:CDS
GATACAAGAGAAGTGCAAAG+AGG 0.678975 1.1:-60326760 MS.gene005143:CDS
GGAAGGAAATAGCAACAACA+AGG 0.682423 1.1:-60326825 MS.gene005143:CDS
GAATGTGGCTGAAGAAAATG+TGG 0.683811 1.1:-60326567 MS.gene005143:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGCTCATATTATTAATTAT+TGG + chr1.1:60326709-60326728 None:intergenic 15.0%
!!! AATCATAATCAAAATAGTTT+TGG - chr1.1:60327145-60327164 MS.gene005143:CDS 15.0%
!!! TCTATGATTAATTGTTTTTT+TGG - chr1.1:60327006-60327025 MS.gene005143:CDS 15.0%
!!! TTTTTGGATTTACATATTTA+CGG - chr1.1:60327022-60327041 MS.gene005143:CDS 15.0%
!! TTGTTAATGACTTGATTAAA+TGG + chr1.1:60326681-60326700 None:intergenic 20.0%
!!! ATAGTTTTGGTAAGAATTTT+AGG - chr1.1:60327158-60327177 MS.gene005143:CDS 20.0%
!!! TATTAAAAAAAGTATCCATG+TGG - chr1.1:60326820-60326839 MS.gene005143:CDS 20.0%
! ATAAGGCTATATGAAGAAAT+AGG + chr1.1:60327862-60327881 None:intergenic 25.0%
! GAATAACAAAAATCTAGTTG+AGG - chr1.1:60327225-60327244 MS.gene005143:CDS 25.0%
! TAAGGCTATATGAAGAAATA+GGG + chr1.1:60327861-60327880 None:intergenic 25.0%
! TTAAGAAAAGAAGAAATTCG+TGG + chr1.1:60326767-60326786 None:intergenic 25.0%
! TTGAGCAATCTTTATAATCA+AGG - chr1.1:60328168-60328187 MS.gene005143:intron 25.0%
!! AATTCTGTTCCTTCAATTTT+TGG + chr1.1:60326925-60326944 None:intergenic 25.0%
!! GATAGATTTGAAGTGTTTAA+GGG - chr1.1:60327400-60327419 MS.gene005143:CDS 25.0%
!! TGATAGATTTGAAGTGTTTA+AGG - chr1.1:60327399-60327418 MS.gene005143:CDS 25.0%
AACAACAATGAAGAAAAGGA+AGG - chr1.1:60327961-60327980 MS.gene005143:intron 30.0%
CAACAACAACAATGAAGAAA+AGG - chr1.1:60327957-60327976 MS.gene005143:intron 30.0%
CAAGTACTTCAGAAAAACAA+AGG - chr1.1:60328092-60328111 MS.gene005143:intron 30.0%
GAAAAACAAAGGATGCTAAT+AGG - chr1.1:60328103-60328122 MS.gene005143:intron 30.0%
GAAATAGGACCAAAAATTGA+AGG - chr1.1:60326913-60326932 MS.gene005143:CDS 30.0%
GTCTTTGATCTCTTTCAATT+TGG + chr1.1:60326498-60326517 None:intergenic 30.0%
TGATTAAATGGATGAAGTGA+AGG + chr1.1:60326669-60326688 None:intergenic 30.0%
TTCAAGAATGGATGATGTAT+TGG - chr1.1:60327300-60327319 MS.gene005143:CDS 30.0%
TTGCTCAATAGATGAAAGTA+AGG + chr1.1:60328156-60328175 None:intergenic 30.0%
!!! AGAGTTTTTTCAAGAGTTTC+CGG + chr1.1:60327760-60327779 None:intergenic 30.0%
AAATCTAGTTGAGGCTGAAA+AGG - chr1.1:60327234-60327253 MS.gene005143:CDS 35.0%
AAGTAGTAGAAGAAGAAGGA+AGG + chr1.1:60327813-60327832 None:intergenic 35.0%
ACACAAGATGAAAACCAAGA+TGG - chr1.1:60328273-60328292 MS.gene005143:CDS 35.0%
ACCAAAGTAGTAGAAGAAGA+AGG + chr1.1:60327817-60327836 None:intergenic 35.0%
AGAAAGCAATAACGTGAATG+TGG - chr1.1:60328221-60328240 MS.gene005143:CDS 35.0%
AGAACTTCATCATTAGTCCA+AGG + chr1.1:60326544-60326563 None:intergenic 35.0%
AGTAGTAGAAGAAGAAGGAA+GGG + chr1.1:60327812-60327831 None:intergenic 35.0%
AGTTTAGTAGAGAAGAAGCT+TGG - chr1.1:60327515-60327534 MS.gene005143:CDS 35.0%
CAAAGGATGCTAATAGGAAA+AGG - chr1.1:60328109-60328128 MS.gene005143:intron 35.0%
CTACAAATGATGAGAAGAAG+AGG - chr1.1:60327362-60327381 MS.gene005143:CDS 35.0%
GAGAATTTCACAAGGAATGT+TGG - chr1.1:60328014-60328033 MS.gene005143:intron 35.0%
GTCAAAGAAAAGTGAAGAAG+AGG - chr1.1:60327324-60327343 MS.gene005143:CDS 35.0%
TCCTTCTTCTTCTACTACTT+TGG - chr1.1:60327813-60327832 MS.gene005143:intron 35.0%
TGAAGATGACATTGAAGCTA+AGG + chr1.1:60327671-60327690 None:intergenic 35.0%
TTCTTCTTCTACTACTTTGG+TGG - chr1.1:60327816-60327835 MS.gene005143:intron 35.0%
TTTCAAGAGTTTCCGGATTA+GGG + chr1.1:60327753-60327772 None:intergenic 35.0%
! AATGCCAACACTTCATCTTT+TGG + chr1.1:60327922-60327941 None:intergenic 35.0%
!! TGTTGGCATTGATAAACTTG+AGG - chr1.1:60327932-60327951 MS.gene005143:intron 35.0%
!! TTTTCAAGAGTTTCCGGATT+AGG + chr1.1:60327754-60327773 None:intergenic 35.0%
!!! TTTTAGGTTTGTTACTGAGC+TGG - chr1.1:60327174-60327193 MS.gene005143:CDS 35.0%
AAGAGGTTTATCAAGGTGGT+GGG - chr1.1:60327197-60327216 MS.gene005143:CDS 40.0%
ACATGTTCCCAAGTGAAATC+TGG + chr1.1:60326607-60326626 None:intergenic 40.0%
AGCTTGGAAGAAACAAGAGA+TGG - chr1.1:60327531-60327550 MS.gene005143:CDS 40.0%
ATGGCCAAAAGATGAAGTGT+TGG - chr1.1:60327915-60327934 MS.gene005143:intron 40.0%
CATGTTCCCAAGTGAAATCT+GGG + chr1.1:60326606-60326625 None:intergenic 40.0%
GAAGAAGAGGTAGTTGAGAA+AGG - chr1.1:60327337-60327356 MS.gene005143:CDS 40.0%
GAATGTGGCTGAAGAAAATG+TGG - chr1.1:60328236-60328255 MS.gene005143:CDS 40.0%
GAGAAGAAGAGGAAAAGATC+AGG - chr1.1:60327373-60327392 MS.gene005143:CDS 40.0%
GAGACGATATTGGTAGAAGA+TGG - chr1.1:60327896-60327915 MS.gene005143:intron 40.0%
GAGTGTGGTGAAGAAAATGA+TGG - chr1.1:60327432-60327451 MS.gene005143:CDS 40.0%
GGAAGGAAATAGCAACAACA+AGG - chr1.1:60327978-60327997 MS.gene005143:intron 40.0%
GTGTTTGTGAGCTGAATTGA+AGG + chr1.1:60326866-60326885 None:intergenic 40.0%
TACATATTTACGGTGTGCAC+AGG - chr1.1:60327032-60327051 MS.gene005143:CDS 40.0%
TCACAAGGAATGTTGGAGTT+AGG - chr1.1:60328021-60328040 MS.gene005143:intron 40.0%
TGTAGAGGAGGATTCAAGAA+TGG - chr1.1:60327288-60327307 MS.gene005143:CDS 40.0%
TGTGAAATTCTCTCCCAAAG+TGG + chr1.1:60328006-60328025 None:intergenic 40.0%
TTCCTCAACTCATTGATCCT+TGG - chr1.1:60326524-60326543 MS.gene005143:CDS 40.0%
TTGAATCCTCCTCTACATGA+GGG + chr1.1:60327285-60327304 None:intergenic 40.0%
TTGGTTCCCAGATTTCACTT+GGG - chr1.1:60326597-60326616 MS.gene005143:CDS 40.0%
TTTGAAGATCAGATCCAGCA+TGG - chr1.1:60326570-60326589 MS.gene005143:CDS 40.0%
! AAAGAGGTGCAAAGAGAAGT+GGG - chr1.1:60328059-60328078 MS.gene005143:intron 40.0%
! ACGTAGTGCTGAAAAATGCA+AGG - chr1.1:60327078-60327097 MS.gene005143:CDS 40.0%
! CTTTGGGAGAGAATTTCACA+AGG - chr1.1:60328006-60328025 MS.gene005143:intron 40.0%
! GATACAAGAGAAGTGCAAAG+AGG - chr1.1:60328043-60328062 MS.gene005143:intron 40.0%
! TAAGGGTTTTTGTGAGAGTG+TGG - chr1.1:60327417-60327436 MS.gene005143:CDS 40.0%
!! GAACAAAGAGCTTGAGTTGA+TGG - chr1.1:60327561-60327580 MS.gene005143:CDS 40.0%
!! TTTTGGTCCTATTTCTCTCG+AGG + chr1.1:60326908-60326927 None:intergenic 40.0%
AAGAAGGAAGGGTTGAGCTA+GGG + chr1.1:60327801-60327820 None:intergenic 45.0%
ACACACACCTCGAGAGAAAT+AGG - chr1.1:60326898-60326917 MS.gene005143:CDS 45.0%
AGAGGTTTATCAAGGTGGTG+GGG - chr1.1:60327198-60327217 MS.gene005143:CDS 45.0%
CAAAATGGACCCTCATGTAG+AGG - chr1.1:60327273-60327292 MS.gene005143:CDS 45.0%
CTGGAAGAGGTTTATCAAGG+TGG - chr1.1:60327193-60327212 MS.gene005143:CDS 45.0%
CTTGAATCCTCCTCTACATG+AGG + chr1.1:60327286-60327305 None:intergenic 45.0%
GAAATAGGGTTGTTCTTGCG+AGG + chr1.1:60327847-60327866 None:intergenic 45.0%
GAACGAGGTGCAAGACAAAA+TGG - chr1.1:60327258-60327277 MS.gene005143:CDS 45.0%
GAAGAGGTTTATCAAGGTGG+TGG - chr1.1:60327196-60327215 MS.gene005143:CDS 45.0%
GAGCTAGGGTTTACAGGTAT+TGG + chr1.1:60327787-60327806 None:intergenic 45.0%
GAGCTGGAAGAGGTTTATCA+AGG - chr1.1:60327190-60327209 MS.gene005143:CDS 45.0%
GATGAAAACCAAGATGGTGC+TGG - chr1.1:60328279-60328298 MS.gene005143:CDS 45.0%
GCACATGAACAAGCTATTGC+TGG - chr1.1:60327583-60327602 MS.gene005143:CDS 45.0%
GCACTCACAAAACCCTAATC+CGG - chr1.1:60327738-60327757 MS.gene005143:CDS 45.0%
GTCCAAGGATCAATGAGTTG+AGG + chr1.1:60326529-60326548 None:intergenic 45.0%
GTGTGTCTTTGTCTACCACA+TGG + chr1.1:60326838-60326857 None:intergenic 45.0%
TCACTTGGGAACATGTCTCA+AGG - chr1.1:60326611-60326630 MS.gene005143:CDS 45.0%
! CAAAGAGGTGCAAAGAGAAG+TGG - chr1.1:60328058-60328077 MS.gene005143:intron 45.0%
! CAACAACAAGGCACCACTTT+GGG - chr1.1:60327990-60328009 MS.gene005143:intron 45.0%
! CAGGTGAGTTTTCTCAAGTG+GGG - chr1.1:60328304-60328323 MS.gene005143:CDS 45.0%
! GCAGGTGAGTTTTCTCAAGT+GGG - chr1.1:60328303-60328322 MS.gene005143:CDS 45.0%
! GTTGGTTCCCAGATTTCACT+TGG - chr1.1:60326596-60326615 MS.gene005143:CDS 45.0%
! GTTTGTTACTGAGCTGGAAG+AGG - chr1.1:60327180-60327199 MS.gene005143:CDS 45.0%
! TGCAGGTGAGTTTTCTCAAG+TGG - chr1.1:60328302-60328321 MS.gene005143:CDS 45.0%
AATGGACCCTCATGTAGAGG+AGG - chr1.1:60327276-60327295 MS.gene005143:CDS 50.0%
AGCAGCGAGAGAGACGATAT+TGG - chr1.1:60327886-60327905 MS.gene005143:intron 50.0%
AGGGTTGAGCTAGGGTTTAC+AGG + chr1.1:60327793-60327812 None:intergenic 50.0%
CTCTCTCGCTGCTCGATATA+AGG + chr1.1:60327879-60327898 None:intergenic 50.0%
GAAGAAGGAAGGGTTGAGCT+AGG + chr1.1:60327802-60327821 None:intergenic 50.0%
TCAGATCCAGCATGGAGAGT+TGG - chr1.1:60326578-60326597 MS.gene005143:CDS 50.0%
! GAGGTTTATCAAGGTGGTGG+GGG - chr1.1:60327199-60327218 MS.gene005143:CDS 50.0%
! GCAACAACAAGGCACCACTT+TGG - chr1.1:60327989-60328008 MS.gene005143:intron 50.0%
CACAGGAAGCTAGCTGACGT+TGG - chr1.1:60327049-60327068 MS.gene005143:CDS 55.0%
TGAGGCTGAAAAGGCGAACG+AGG - chr1.1:60327243-60327262 MS.gene005143:CDS 55.0%
TGGGAACCAACTCTCCATGC+TGG + chr1.1:60326587-60326606 None:intergenic 55.0%
!! AACCAAGATGGTGCTGGTGC+AGG - chr1.1:60328285-60328304 MS.gene005143:CDS 55.0%
CACCTGCACCAGCACCATCT+TGG + chr1.1:60328290-60328309 None:intergenic 60.0%
CTCTCGAGGTGTGTGTCTCG+TGG + chr1.1:60326894-60326913 None:intergenic 60.0%
CTCAAGTGGGGCCTCCAAGC+TGG - chr1.1:60328316-60328335 MS.gene005143:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 60326484 60328341 60326484 ID=MS.gene005143
chr1.1 mRNA 60326484 60328341 60326484 ID=MS.gene005143.t1;Parent=MS.gene005143
chr1.1 exon 60328193 60328341 60328193 ID=MS.gene005143.t1.exon1;Parent=MS.gene005143.t1
chr1.1 CDS 60328193 60328341 60328193 ID=cds.MS.gene005143.t1;Parent=MS.gene005143.t1
chr1.1 exon 60326484 60327771 60326484 ID=MS.gene005143.t1.exon2;Parent=MS.gene005143.t1
chr1.1 CDS 60326484 60327771 60326484 ID=cds.MS.gene005143.t1;Parent=MS.gene005143.t1
Gene Sequence

>MS.gene005143

ATGAACAACTTCCAAATTGAAAGAGATCAAAGACAAATACTTCCTCAACTCATTGATCCTTGGACTAATGATGAAGTTCTTGCACTTTTGAAGATCAGATCCAGCATGGAGAGTTGGTTCCCAGATTTCACTTGGGAACATGTCTCAAGGTATACAAATATATCATTGTAATTCTAACATCTCCTTCACTTCATCCATTTAATCAAGTCATTAACAATCATACCAATAATTAATAATATGAGCTAGATATTTATAAATCTTAACTAAATGTATATTCTTTCCACGAATTTCTTCTTTTCTTAATACTACTAGTTTTATTAACATTTGCGTGAGTATTATTAAAAAAAGTATCCATGTGGTAGACAAAGACACACGTGCACCTTCAATTCAGCTCACAAACACTGACACCACGAGACACACACCTCGAGAGAAATAGGACCAAAAATTGAAGGAACAGAATTTGTCTATTATTACTATGTATTGAATACGCTTGTGTGTTAGAGTAATTCAATGCTTTTTAATTCTATGATTAATTGTTTTTTTGGATTTACATATTTACGGTGTGCACAGGAAGCTAGCTGACGTTGGTTATAAACGTAGTGCTGAAAAATGCAAGGAAAAATTTGAAGAAGAAAGCAGATTCTTCAACAACATTAATAATAATCATAATCAAAATAGTTTTGGTAAGAATTTTAGGTTTGTTACTGAGCTGGAAGAGGTTTATCAAGGTGGTGGGGGTGAGAATAACAAAAATCTAGTTGAGGCTGAAAAGGCGAACGAGGTGCAAGACAAAATGGACCCTCATGTAGAGGAGGATTCAAGAATGGATGATGTATTGGTGTCAAAGAAAAGTGAAGAAGAGGTAGTTGAGAAAGGTACTACAAATGATGAGAAGAAGAGGAAAAGATCAGGTGATGATAGATTTGAAGTGTTTAAGGGTTTTTGTGAGAGTGTGGTGAAGAAAATGATGGATCAACAAGAAGAAATGCACAACAAGCTTATTGAAGATATGATTAAAAGAGATGAAGAGAAGTTTAGTAGAGAAGAAGCTTGGAAGAAACAAGAGATGGAGAAAATGAACAAAGAGCTTGAGTTGATGGCACATGAACAAGCTATTGCTGGTGATAGACAATCACATATAATTCAATTCTTGAACAAATTCTCAACAAGTGCTAATTCTTCTTCCTTAGCTTCAATGTCATCTTCACTTCAAGCTTATTTAGCAACATTAACTTCAAACTCTTCTTCTACATTGCACTCACAAAACCCTAATCCGGAAACTCTTGAAAAAACTCTTCAACCAATACCTGTAAACCCTAGCTCAACCCTTCCTTCTTCTTCTACTACTTTGGTGGCTCAGCCTCGCAAGAACAACCCTATTTCTTCATATAGCCTTATATCGAGCAGCGAGAGAGACGATATTGGTAGAAGATGGCCAAAAGATGAAGTGTTGGCATTGATAAACTTGAGGTGCAACAACAACAATGAAGAAAAGGAAGGAAATAGCAACAACAAGGCACCACTTTGGGAGAGAATTTCACAAGGAATGTTGGAGTTAGGATACAAGAGAAGTGCAAAGAGGTGCAAAGAGAAGTGGGAGAATATAAACAAGTACTTCAGAAAAACAAAGGATGCTAATAGGAAAAGGTCACTTGATTCAAGAACTTGTCCTTACTTTCATCTATTGAGCAATCTTTATAATCAAGGTAAGCTTGTTCTTCAATCTGATCAAAAGCAAGAAAGCAATAACGTGAATGTGGCTGAAGAAAATGTGGTTCAAGAGAAAGCAACACAAGATGAAAACCAAGATGGTGCTGGTGCAGGTGAGTTTTCTCAAGTGGGGCCTCCAAGCTGGTAA
Protein sequence

>MS.gene005143.t1

MNNFQIERDQRQILPQLIDPWTNDEVLALLKIRSSMESWFPDFTWEHVSRKLADVGYKRSAEKCKEKFEEESRFFNNINNNHNQNSFGKNFRFVTELEEVYQGGGGENNKNLVEAEKANEVQDKMDPHVEEDSRMDDVLVSKKSEEEVVEKGTTNDEKKRKRSGDDRFEVFKGFCESVVKKMMDQQEEMHNKLIEDMIKRDEEKFSREEAWKKQEMEKMNKELELMAHEQAIAGDRQSHIIQFLNKFSTSANSSSLASMSSSLQAYLATLTSNSSSTLHSQNPNPETLEKTLQPIPVNPSSTLPSSSTTLVAQPRKNNPISSYSLISSSERDDIGRRWPKDEVLALINLRCNNNNEEKEGNSNNKAPLWERISQGMLELGYKRSAKRCKEKWENINKYFRKTKDANRKRSLDSRTCPYFHLLSNLYNQGKLVLQSDQKQESNNVNVAEENVVQEKATQDENQDGAGAGEFSQVGPPSW