AlfalfaGEDB | MS.gene005241

M. sativa cultivar XinJiangDaYe / MS.gene005241

KEGG: K08065
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0046982 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005241.t1	XP_003591124.1	97.6	126	3	0	1	126	1	126	3.70E-65	257.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005241.t1	O04027	63.5	126	41	2	1	122	1	125	1.8e-36	153.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005241.t1	G7I854	97.6	126	3	0	1	126	1	126	2.7e-65	257.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene005241.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005241.t1	MTR_1g083070	97.619	126	3	0	1	126	1	126	2.39e-92	262
MS.gene005241.t1	MTR_1g088860	72.632	95	26	0	4	98	36	130	1.01e-49	157
MS.gene005241.t1	MTR_0392s0020	68.687	99	31	0	4	102	18	116	7.38e-48	150
MS.gene005241.t1	MTR_4g119500	71.579	95	27	0	4	98	22	116	7.32e-47	147
MS.gene005241.t1	MTR_4g133938	55.752	113	44	1	6	112	21	133	1.15e-41	134
MS.gene005241.t1	MTR_2g026710	63.043	92	34	0	4	95	17	108	1.23e-39	130
MS.gene005241.t1	MTR_3g058980	60.870	92	36	0	4	95	27	118	7.50e-39	129
MS.gene005241.t1	MTR_8g093920	59.794	97	39	0	4	100	40	136	1.00e-38	129
MS.gene005241.t1	MTR_5g095740	60.870	92	36	0	4	95	23	114	2.47e-38	127
MS.gene005241.t1	MTR_7g061270	45.968	124	67	0	4	127	26	149	3.02e-38	127
MS.gene005241.t1	MTR_7g100650	45.968	124	67	0	4	127	26	149	4.45e-38	127
MS.gene005241.t1	MTR_8g091720	60.870	92	36	0	4	95	28	119	4.71e-38	127
MS.gene005241.t1	MTR_1g072790	56.522	92	40	0	4	95	29	120	2.88e-36	122
MS.gene005241.t1	MTR_4g133952	51.087	92	45	0	4	95	58	149	2.81e-33	116
MS.gene005241.t1	MTR_1g039040	51.087	92	45	0	4	95	6	97	1.97e-31	110
MS.gene005241.t1	MTR_5g095900	43.564	101	55	1	1	101	13	111	7.82e-23	88.2
MS.gene005241.t1	MTR_1g029070	41.379	87	51	0	9	95	57	143	5.06e-20	80.9
MS.gene005241.t1	MTR_1g029100	35.000	80	52	0	9	88	7	86	2.86e-17	71.6
MS.gene005241.t1	MTR_2g056000	35.443	79	51	0	6	84	43	121	1.35e-15	69.7
MS.gene005241.t1	MTR_4g112380	31.646	79	54	0	6	84	13	91	1.08e-13	63.9
MS.gene005241.t1	MTR_1g028480	42.105	57	33	0	39	95	24	80	2.20e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005241.t1	AT1G09030	63.492	126	41	2	1	122	1	125	6.21e-51	158
MS.gene005241.t1	AT2G47810	68.421	95	30	0	1	95	48	142	1.99e-45	145
MS.gene005241.t1	AT5G47640	64.130	92	33	0	4	95	27	118	1.92e-40	133
MS.gene005241.t1	AT4G14540	60.870	92	36	0	4	95	21	112	2.61e-39	129
MS.gene005241.t1	AT2G13570	59.000	100	41	0	4	103	36	135	1.13e-38	129
MS.gene005241.t1	AT2G38880	58.696	92	38	0	4	95	21	112	7.32e-38	125
MS.gene005241.t1	AT2G38880	58.696	92	38	0	4	95	21	112	9.08e-38	125
MS.gene005241.t1	AT2G38880	58.696	92	38	0	4	95	21	112	9.08e-38	125
MS.gene005241.t1	AT2G38880	58.696	92	38	0	4	95	21	112	9.08e-38	125
MS.gene005241.t1	AT2G38880	58.242	91	38	0	4	94	21	111	1.07e-37	124
MS.gene005241.t1	AT2G38880	57.609	92	39	0	4	95	21	112	1.32e-37	124
MS.gene005241.t1	AT2G37060	56.522	92	40	0	4	95	30	121	2.79e-37	125
MS.gene005241.t1	AT2G37060	56.522	92	40	0	4	95	30	121	2.79e-37	125
MS.gene005241.t1	AT2G37060	56.522	92	40	0	4	95	30	121	2.79e-37	125
MS.gene005241.t1	AT3G53340	56.044	91	40	0	4	94	29	119	3.19e-37	123
MS.gene005241.t1	AT3G53340	55.914	93	41	0	4	96	29	121	3.62e-37	124
MS.gene005241.t1	AT3G53340	55.914	93	41	0	4	96	29	121	3.62e-37	124
MS.gene005241.t1	AT3G53340	56.522	92	40	0	4	95	29	120	4.30e-37	124
MS.gene005241.t1	AT3G53340	56.522	92	40	0	4	95	29	120	4.30e-37	124
MS.gene005241.t1	AT2G38880	58.242	91	38	0	4	94	21	111	5.81e-37	123
MS.gene005241.t1	AT2G38880	58.242	91	38	0	4	94	21	111	5.81e-37	123
MS.gene005241.t1	AT2G38880	58.242	91	38	0	4	94	21	111	5.81e-37	123
MS.gene005241.t1	AT2G38880	58.242	91	38	0	4	94	21	111	6.01e-37	124
MS.gene005241.t1	AT2G38880	58.242	91	38	0	4	94	21	111	6.01e-37	124
MS.gene005241.t1	AT5G47670	51.087	92	45	0	4	95	29	120	1.42e-32	114
MS.gene005241.t1	AT5G47670	51.087	92	45	0	4	95	58	149	3.53e-32	113
MS.gene005241.t1	AT5G47670	51.087	92	45	0	4	95	58	149	3.53e-32	113
MS.gene005241.t1	AT1G21970	49.474	95	48	0	4	98	59	153	2.90e-31	111
MS.gene005241.t1	AT5G23090	36.709	79	50	0	6	84	13	91	1.52e-16	71.6
MS.gene005241.t1	AT5G23090	36.709	79	50	0	6	84	13	91	1.68e-16	71.2
MS.gene005241.t1	AT5G23090	36.709	79	50	0	6	84	13	91	1.68e-16	71.2
MS.gene005241.t1	AT5G23090	36.709	79	50	0	6	84	13	91	1.68e-16	71.2
MS.gene005241.t1	AT5G08190	32.911	79	53	0	6	84	13	91	1.26e-14	66.6
MS.gene005241.t1	AT5G08190	32.911	79	53	0	6	84	13	91	1.34e-14	66.6

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGAATCGCAAGACAGTTAA+TGG	0.277173	1.4:-67176687	MS.gene005241:CDS
TGTTGGGCTTTGTGTTCGTT+AGG	0.349672	1.4:-67176654	MS.gene005241:CDS
TGACCACTATGTTTGGTACT+TGG	0.392179	1.4:+67176504	None:intergenic
GACAACTATGCTGAAGCTAT+TGG	0.407603	1.4:-67176627	MS.gene005241:CDS
TTACAGATGACCACTATGTT+TGG	0.421378	1.4:+67176497	None:intergenic
AGAATCGCAAGACAGTTAAT+GGG	0.432736	1.4:-67176686	MS.gene005241:CDS
TATTCTACCCACATTTGCTA+TGG	0.454852	1.4:+67176830	None:intergenic
GTTGGGCTTTGTGTTCGTTA+GGG	0.471790	1.4:-67176653	MS.gene005241:CDS
AACGTTGCCCATAGCAAATG+TGG	0.502850	1.4:-67176838	MS.gene005241:CDS
TAATGGGGATGACATCTGTT+GGG	0.508909	1.4:-67176670	MS.gene005241:CDS
ATTCTACCCACATTTGCTAT+GGG	0.509383	1.4:+67176831	None:intergenic
GAGTTCATAAGTTTCGTGAC+AGG	0.522754	1.4:-67176735	MS.gene005241:CDS
TTAATGGGGATGACATCTGT+TGG	0.554383	1.4:-67176671	MS.gene005241:CDS
TTTGAGATCTTTGCAGTGGG+AGG	0.587845	1.4:+67176792	None:intergenic
TAGCTAGAAATGAACGATGA+GGG	0.603562	1.4:-67176867	None:intergenic
ACTATGCTGAAGCTATTGGA+AGG	0.633037	1.4:-67176623	MS.gene005241:CDS
AGCATCTGACAAGTGTCACA+AGG	0.642405	1.4:-67176709	MS.gene005241:CDS
AATCCAAGTACCAAACATAG+TGG	0.647897	1.4:-67176507	MS.gene005241:CDS
GTAGCTAGAAATGAACGATG+AGG	0.648734	1.4:-67176868	None:intergenic
ACGTTGCCCATAGCAAATGT+GGG	0.656251	1.4:-67176837	MS.gene005241:CDS
GAATCGCAAGACAGTTAATG+GGG	0.668357	1.4:-67176685	MS.gene005241:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATCCAAGTACCAAACATAG+TGG	-	chr1.4:67176848-67176867	MS.gene005241:CDS	35.0%
	AGAATCGCAAGACAGTTAAT+GGG	-	chr1.4:67176669-67176688	MS.gene005241:CDS	35.0%
	TTACAGATGACCACTATGTT+TGG	+	chr1.4:67176861-67176880	None:intergenic	35.0%
!	TATTCTACCCACATTTGCTA+TGG	+	chr1.4:67176528-67176547	None:intergenic	35.0%
!!	ATTCTACCCACATTTGCTAT+GGG	+	chr1.4:67176527-67176546	None:intergenic	35.0%
!!!	TCTTTTGAGATCTTTGCAGT+GGG	+	chr1.4:67176569-67176588	None:intergenic	35.0%
!!!	TTCTTTTGAGATCTTTGCAG+TGG	+	chr1.4:67176570-67176589	None:intergenic	35.0%
	ACTATGCTGAAGCTATTGGA+AGG	-	chr1.4:67176732-67176751	MS.gene005241:CDS	40.0%
	GAATCGCAAGACAGTTAATG+GGG	-	chr1.4:67176670-67176689	MS.gene005241:CDS	40.0%
	GACAACTATGCTGAAGCTAT+TGG	-	chr1.4:67176728-67176747	MS.gene005241:CDS	40.0%
	GAGAATCGCAAGACAGTTAA+TGG	-	chr1.4:67176668-67176687	MS.gene005241:CDS	40.0%
	GAGTTCATAAGTTTCGTGAC+AGG	-	chr1.4:67176620-67176639	MS.gene005241:CDS	40.0%
	TAATGGGGATGACATCTGTT+GGG	-	chr1.4:67176685-67176704	MS.gene005241:CDS	40.0%
	TGACCACTATGTTTGGTACT+TGG	+	chr1.4:67176854-67176873	None:intergenic	40.0%
	TTAATGGGGATGACATCTGT+TGG	-	chr1.4:67176684-67176703	MS.gene005241:CDS	40.0%
	AACGTTGCCCATAGCAAATG+TGG	-	chr1.4:67176517-67176536	MS.gene005241:CDS	45.0%
	ACGTTGCCCATAGCAAATGT+GGG	-	chr1.4:67176518-67176537	MS.gene005241:CDS	45.0%
	AGCATCTGACAAGTGTCACA+AGG	-	chr1.4:67176646-67176665	MS.gene005241:CDS	45.0%
!	GTTGGGCTTTGTGTTCGTTA+GGG	-	chr1.4:67176702-67176721	MS.gene005241:CDS	45.0%
!	TGTTGGGCTTTGTGTTCGTT+AGG	-	chr1.4:67176701-67176720	MS.gene005241:CDS	45.0%
!	TTTGAGATCTTTGCAGTGGG+AGG	+	chr1.4:67176566-67176585	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	67176497	67176880	67176497	ID=MS.gene005241
chr1.4	mRNA	67176497	67176880	67176497	ID=MS.gene005241.t1;Parent=MS.gene005241
chr1.4	exon	67176497	67176880	67176497	ID=MS.gene005241.t1.exon1;Parent=MS.gene005241.t1
chr1.4	CDS	67176497	67176880	67176497	ID=cds.MS.gene005241.t1;Parent=MS.gene005241.t1

Gene Sequence

>MS.gene005241

ATGAACGATGAGGGAGATAAAACGTTGCCCATAGCAAATGTGGGTAGAATAATGAAGCAAAATCTTCCTCCCACTGCAAAGATCTCAAAAGAAAGCAAACAGTTGATGCAAGAATGTGCGACAGAGTTCATAAGTTTCGTGACAGGTGAAGCATCTGACAAGTGTCACAAGGAGAATCGCAAGACAGTTAATGGGGATGACATCTGTTGGGCTTTGTGTTCGTTAGGGTTTGACAACTATGCTGAAGCTATTGGAAGGTACTTGTATAAATTCAGACAAGCTGAACTTGTTAGAATAAATCAGAACAAACTTCATGAAACTGCAAAAGACAAGTTTGAAGAAGATGCAACAAATCCAAGTACCAAACATAGTGGTCATCTGTAA

Protein sequence

>MS.gene005241.t1

MNDEGDKTLPIANVGRIMKQNLPPTAKISKESKQLMQECATEFISFVTGEASDKCHKENRKTVNGDDICWALCSLGFDNYAEAIGRYLYKFRQAELVRINQNKLHETAKDKFEEDATNPSTKHSGHL