AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005321

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005321.t1 MTR_1g084150 75.966 233 29 2 145 350 1 233 7.06e-107 312
MS.gene005321.t1 MTR_5g095070 58.586 297 93 7 2 298 5 271 3.65e-104 307
MS.gene005321.t1 MTR_5g080030 62.609 230 79 1 32 261 44 266 8.05e-95 288
MS.gene005321.t1 MTR_5g080030 53.254 169 58 6 183 350 264 412 4.54e-38 140
MS.gene005321.t1 MTR_4g015240 51.220 82 19 2 259 339 39 100 7.93e-17 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCGTTGGATATGTTAAA+TGG 0.182684 1.1:+62409439 MS.gene005321:CDS
TACTGGTTTATCTCGATTAA+TGG 0.250811 1.1:+62408396 MS.gene005321:CDS
TCCCTTCTTCCCAGTTGATT+TGG 0.271998 1.1:+62409408 MS.gene005321:CDS
CCTTCGGTGCTGCCGGTTAC+AGG 0.272596 1.1:-62408015 None:intergenic
AGTTGTCTCTGCACTGAAAT+TGG 0.295292 1.1:+62407903 MS.gene005321:CDS
AACAGTACCATTGGTGAGTT+TGG 0.303944 1.1:-62406812 None:intergenic
TGTCAAACTAACTGAAGTTT+TGG 0.307954 1.1:+62406870 MS.gene005321:CDS
TTCTTGCTTTCCTCCACATA+TGG 0.365771 1.1:-62409157 None:intergenic
TTTGTAAAGGTACGCGTAAA+AGG 0.366190 1.1:+62408359 MS.gene005321:intron
TTCAGTTAGTTTGACATAGA+AGG 0.372838 1.1:-62406863 None:intergenic
CAGTAAGTATCCAAATCAAC+TGG 0.378693 1.1:-62409418 None:intergenic
TTCTACAGGTTTCCTGTAAC+CGG 0.383374 1.1:+62408003 MS.gene005321:CDS
GAAGAGCACTCAAGATCTTA+AGG 0.392322 1.1:+62409258 MS.gene005321:CDS
AGGTTGGTCTTCAAAACAAA+TGG 0.395740 1.1:+62407875 MS.gene005321:intron
TTGAGAAATTGTACTTCTAC+AGG 0.399495 1.1:+62407989 MS.gene005321:CDS
AATCCTTATAACCTGTCATT+TGG 0.408344 1.1:-62408468 None:intergenic
CAACCGCTAGTATAGTCATT+TGG 0.413628 1.1:-62409467 None:intergenic
CTCTATCTCTAAGTCATCAT+TGG 0.415656 1.1:-62406839 None:intergenic
GGAGGAAAGCAAGAAGATAA+AGG 0.418994 1.1:+62409165 MS.gene005321:CDS
TGGTGACTTCCAAGTAAAAC+AGG 0.431064 1.1:-62407578 None:intergenic
AATGGAAGGCAACAAGACTA+TGG 0.438680 1.1:+62406765 None:intergenic
CTGGTGAAAACCTCCTCTTC+TGG 0.440306 1.1:-62407598 None:intergenic
ATCCAAATCAACTGGGAAGA+AGG 0.454997 1.1:-62409410 None:intergenic
CACCCAAGGTTCTTGACTGC+TGG 0.455306 1.1:-62408805 None:intergenic
GCTCATGATCCGGACATTGA+TGG 0.456058 1.1:+62408957 MS.gene005321:CDS
GTTCGTTGGATATGTTAAAT+GGG 0.459203 1.1:+62409440 MS.gene005321:CDS
TACGCATGGCTGTTGATGCT+TGG 0.460942 1.1:+62408991 MS.gene005321:CDS
AAGACAAAGAAAAGAAGAAA+CGG 0.466286 1.1:+62408868 MS.gene005321:CDS
TATTTGATATCCACTTTGTC+CGG 0.466627 1.1:-62408904 None:intergenic
AGCCAGCAGTCAAGAACCTT+GGG 0.467633 1.1:+62408803 MS.gene005321:CDS
TTTGGATACTTACTGTTCGT+TGG 0.489477 1.1:+62409426 MS.gene005321:CDS
TTAGATACCTTCGGTGCTGC+CGG 0.491330 1.1:-62408022 None:intergenic
ACAGAAGAATAGTTTGGTAG+TGG 0.492096 1.1:-62409331 None:intergenic
AGGAAGCAGAACTCGACTAC+TGG 0.510036 1.1:+62408379 MS.gene005321:CDS
GATGTCTGCCACTGATAAAG+AGG 0.517133 1.1:+62409019 MS.gene005321:CDS
TTGTTGACAGAAGAATAGTT+TGG 0.526138 1.1:-62409337 None:intergenic
TCCAAATCAACTGGGAAGAA+GGG 0.526508 1.1:-62409409 None:intergenic
CAACAATGCAGCTGTTGAGT+TGG 0.535181 1.1:+62409354 MS.gene005321:CDS
TACTTGGAAGTCACCAGAAG+AGG 0.540823 1.1:+62407585 MS.gene005321:CDS
GGTTGGTCTTCAAAACAAAT+GGG 0.543380 1.1:+62407876 MS.gene005321:intron
GAGCCAGCAGTCAAGAACCT+TGG 0.543603 1.1:+62408802 MS.gene005321:CDS
TCGATTAAAAGCTCATGATC+CGG 0.546735 1.1:+62408947 MS.gene005321:CDS
GAGCACTGAAGATCTTAAGA+AGG 0.550700 1.1:+62409228 MS.gene005321:CDS
TGATATCCACTTTGTCCGGT+AGG 0.555314 1.1:-62408900 None:intergenic
CCTGTAACCGGCAGCACCGA+AGG 0.559034 1.1:+62408015 MS.gene005321:CDS
ATTGGACACAACAGTACCAT+TGG 0.564594 1.1:-62406821 None:intergenic
GTCTCTGCACTGAAATTGGA+AGG 0.566571 1.1:+62407907 MS.gene005321:CDS
AAGATCTTAAGGAGGAAAAG+AGG 0.571062 1.1:+62409269 MS.gene005321:CDS
AAACGGCGAGGTCCTCCTAC+CGG 0.572639 1.1:+62408885 MS.gene005321:CDS
TTCCTAGCTCAAACAATGGA+AGG 0.574513 1.1:+62406751 None:intergenic
TAGTCGTAACCGCACACACT+AGG 0.575038 1.1:-62409292 None:intergenic
GGCTACTCTAGCCAAATGAC+AGG 0.588051 1.1:+62408457 MS.gene005321:CDS
TTGGAAGTCACCAGAAGAGG+AGG 0.590853 1.1:+62407588 MS.gene005321:CDS
GTATCTGAAGGTGATGATGA+TGG 0.592975 1.1:-62406790 None:intergenic
TATCCACTTTGTCCGGTAGG+AGG 0.594215 1.1:-62408897 None:intergenic
CATTTGGCTAGAGTAGCCGT+TGG 0.607924 1.1:-62408452 None:intergenic
TTATCATACCTCTTTATCAG+TGG 0.622643 1.1:-62409027 None:intergenic
GGTCCTCCTACCGGACAAAG+TGG 0.623840 1.1:+62408894 MS.gene005321:CDS
GGTGCTTGTAGAAGCACCCA+AGG 0.627728 1.1:-62408819 None:intergenic
ACAAGTCTAAGATTGTCTCT+CGG 0.631739 1.1:-62407554 None:intergenic
GAAAAGAGGCCTAGTGTGTG+CGG 0.639000 1.1:+62409283 MS.gene005321:CDS
TCAGATACCAAACTCACCAA+TGG 0.649186 1.1:+62406805 MS.gene005321:CDS
AATGTGGCAAAGGTGTCCAA+CGG 0.659732 1.1:+62408436 MS.gene005321:CDS
AGATAATCTGAATGTGGCAA+AGG 0.661885 1.1:+62408426 MS.gene005321:CDS
AGTAAGTATCCAAATCAACT+GGG 0.663991 1.1:-62409417 None:intergenic
GAGCACTCAAGATCTTAAGG+AGG 0.665195 1.1:+62409261 MS.gene005321:CDS
TTGGTGAGTTTGGTATCTGA+AGG 0.672081 1.1:-62406802 None:intergenic
TCATGAAGATAATCTGAATG+TGG 0.680646 1.1:+62408420 MS.gene005321:CDS
AAAGAAAAGAAGAAACGGCG+AGG 0.681943 1.1:+62408873 MS.gene005321:CDS
AGACATGAGATTGAACCCAG+AGG 0.774697 1.1:-62406896 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGGATAAAAAAAAATGA+TGG + chr1.1:62407472-62407491 MS.gene005321:intron 15.0%
!!! AAAAAAATGATGGATAATAA+AGG + chr1.1:62407482-62407501 MS.gene005321:intron 15.0%
!!! AAAAAATGATGGATAATAAA+GGG + chr1.1:62407483-62407502 MS.gene005321:intron 15.0%
!!! TAAAGTTTTATGAGATTATT+TGG + chr1.1:62407455-62407474 MS.gene005321:intron 15.0%
!!! TTTTATTACGTTTTTGTTTT+AGG + chr1.1:62408280-62408299 MS.gene005321:intron 15.0%
!! TAATACAAATTAGTCAATAG+AGG + chr1.1:62407780-62407799 MS.gene005321:intron 20.0%
!! TACTTGTGGATAAAAATATT+TGG + chr1.1:62407213-62407232 MS.gene005321:intron 20.0%
!! TTTATTCCTCTTTAAATGTT+TGG + chr1.1:62407368-62407387 MS.gene005321:intron 20.0%
!!! TATAACACATTTCAGTTTTA+AGG - chr1.1:62407347-62407366 None:intergenic 20.0%
!!! TTTTTCTTTTATTGCATGTT+TGG - chr1.1:62407179-62407198 None:intergenic 20.0%
! AAAATGCCTTGTAAAAACAT+TGG + chr1.1:62408181-62408200 MS.gene005321:intron 25.0%
! AAGACAAAGAAAAGAAGAAA+CGG + chr1.1:62408868-62408887 MS.gene005321:CDS 25.0%
! AGAACAAATTAAATCATGTG+AGG - chr1.1:62407651-62407670 None:intergenic 25.0%
! ATGCTTGATATGTAAATGTT+TGG + chr1.1:62408115-62408134 MS.gene005321:intron 25.0%
! ATTTCAAACAAACAAAGTAG+AGG - chr1.1:62409108-62409127 None:intergenic 25.0%
! CTCTTTAAATGTTTGGTATA+AGG + chr1.1:62407375-62407394 MS.gene005321:intron 25.0%
! CTTATACCAAACATTTAAAG+AGG - chr1.1:62407377-62407396 None:intergenic 25.0%
! GAAGATAAAGGAAAAAAAGA+AGG + chr1.1:62409177-62409196 MS.gene005321:CDS 25.0%
! TATAGACACAACATATTTGA+TGG - chr1.1:62407760-62407779 None:intergenic 25.0%
!! ATAAAAATATTTGGTTCGAG+GGG + chr1.1:62407222-62407241 MS.gene005321:intron 25.0%
!! ATAAAGTAACAAACCTGTTT+TGG - chr1.1:62408651-62408670 None:intergenic 25.0%
!! ATGTCTCAATTTTTCTTTTG+TGG - chr1.1:62407306-62407325 None:intergenic 25.0%
!! ATTGGTCTAAGAAAATGTAT+TGG + chr1.1:62408199-62408218 MS.gene005321:intron 25.0%
!! GATAAAAATATTTGGTTCGA+GGG + chr1.1:62407221-62407240 MS.gene005321:intron 25.0%
!! TTTGGATTCTTATTTAAAGC+AGG + chr1.1:62408780-62408799 MS.gene005321:intron 25.0%
!!! TTCTTTTCTTTGTCATTTGA+GGG - chr1.1:62408843-62408862 None:intergenic 25.0%
AAAATTGAAACAGAGTTGCA+TGG - chr1.1:62407516-62407535 None:intergenic 30.0%
AATCCTTATAACCTGTCATT+TGG - chr1.1:62408471-62408490 None:intergenic 30.0%
AGAAAAGAAAGGAGACTTTA+AGG - chr1.1:62408325-62408344 None:intergenic 30.0%
AGACATGCATTTAGTCATAT+TGG + chr1.1:62407320-62407339 MS.gene005321:intron 30.0%
AGTAAGTATCCAAATCAACT+GGG - chr1.1:62409420-62409439 None:intergenic 30.0%
ATTGATCTCTTAGATACCTT+CGG - chr1.1:62408034-62408053 None:intergenic 30.0%
GATTACTAAAGTTCCAAAAC+AGG + chr1.1:62408635-62408654 MS.gene005321:intron 30.0%
GGATGTGAAATTAAATCACT+CGG + chr1.1:62408220-62408239 MS.gene005321:intron 30.0%
GTATCTAAGAGATCAATTTG+TGG + chr1.1:62408037-62408056 MS.gene005321:intron 30.0%
GTTCGTTGGATATGTTAAAT+GGG + chr1.1:62409440-62409459 MS.gene005321:CDS 30.0%
TATTTGATATCCACTTTGTC+CGG - chr1.1:62408907-62408926 None:intergenic 30.0%
TCATGAAGATAATCTGAATG+TGG + chr1.1:62408420-62408439 MS.gene005321:CDS 30.0%
TGTTCGTTGGATATGTTAAA+TGG + chr1.1:62409439-62409458 MS.gene005321:CDS 30.0%
TTATCATACCTCTTTATCAG+TGG - chr1.1:62409030-62409049 None:intergenic 30.0%
TTGAGAAATTGTACTTCTAC+AGG + chr1.1:62407989-62408008 MS.gene005321:CDS 30.0%
TTGGATTGAGGATAAATTTG+TGG - chr1.1:62407160-62407179 None:intergenic 30.0%
TTGTGGCAAAAACTACAAAT+TGG - chr1.1:62407143-62407162 None:intergenic 30.0%
TTGTTGACAGAAGAATAGTT+TGG - chr1.1:62409340-62409359 None:intergenic 30.0%
TTTATTGCATGTTTGGATTG+AGG - chr1.1:62407172-62407191 None:intergenic 30.0%
! ACATATTTGATGGATAGACT+TGG - chr1.1:62407750-62407769 None:intergenic 30.0%
! ATATCGATCTTGTTTCATTC+CGG + chr1.1:62408073-62408092 MS.gene005321:intron 30.0%
! ATTCACTGATTGAATGCAAA+AGG + chr1.1:62408517-62408536 MS.gene005321:intron 30.0%
! GGATAAAAATATTTGGTTCG+AGG + chr1.1:62407220-62407239 MS.gene005321:intron 30.0%
! TCTCAATTTTTCTTTTGTGG+TGG - chr1.1:62407303-62407322 None:intergenic 30.0%
! TGTCAAACTAACTGAAGTTT+TGG + chr1.1:62406870-62406889 MS.gene005321:CDS 30.0%
! TTCAGTTAGTTTGACATAGA+AGG - chr1.1:62406866-62406885 None:intergenic 30.0%
!! CTTAGACCAATGTTTTTACA+AGG - chr1.1:62408190-62408209 None:intergenic 30.0%
!! CTTCTTTTCTTTGTCATTTG+AGG - chr1.1:62408844-62408863 None:intergenic 30.0%
!! TACTGGTTTATCTCGATTAA+TGG + chr1.1:62408396-62408415 MS.gene005321:CDS 30.0%
!!! CTTTTTTATGTAGCCATATG+TGG + chr1.1:62409144-62409163 MS.gene005321:intron 30.0%
AAGATCTTAAGGAGGAAAAG+AGG + chr1.1:62409269-62409288 MS.gene005321:CDS 35.0%
AATCATGTGAGGTCAATATG+TGG - chr1.1:62407640-62407659 None:intergenic 35.0%
AATGTTTGGTATAAGGTCAC+TGG + chr1.1:62407382-62407401 MS.gene005321:intron 35.0%
ACAGAAGAATAGTTTGGTAG+TGG - chr1.1:62409334-62409353 None:intergenic 35.0%
ACTAGCTAGTGACCTTTATT+GGG - chr1.1:62407252-62407271 None:intergenic 35.0%
ACTGCATATAAGTGAAGTAC+CGG - chr1.1:62408095-62408114 None:intergenic 35.0%
AGATAATCTGAATGTGGCAA+AGG + chr1.1:62408426-62408445 MS.gene005321:CDS 35.0%
ATCCAAATATCCAAACATGC+AGG - chr1.1:62407073-62407092 None:intergenic 35.0%
CAGTAAGTATCCAAATCAAC+TGG - chr1.1:62409421-62409440 None:intergenic 35.0%
CTCTATCTCTAAGTCATCAT+TGG - chr1.1:62406842-62406861 None:intergenic 35.0%
CTTTACAAAATGCAAGTGTG+AGG - chr1.1:62408348-62408367 None:intergenic 35.0%
TAGCCAAATGACAGGTTATA+AGG + chr1.1:62408465-62408484 MS.gene005321:intron 35.0%
TCGATTAAAAGCTCATGATC+CGG + chr1.1:62408947-62408966 MS.gene005321:CDS 35.0%
TGAGGTCAATATGTGGATAA+CGG - chr1.1:62407633-62407652 None:intergenic 35.0%
TGTACTTATTAGCTGCAATG+TGG + chr1.1:62408670-62408689 MS.gene005321:intron 35.0%
TGTGAAATTAAATCACTCGG+TGG + chr1.1:62408223-62408242 MS.gene005321:intron 35.0%
TGTGTGCATTATGCTATGTT+TGG + chr1.1:62406993-62407012 MS.gene005321:intron 35.0%
TTCCTGCATGTTTGGATATT+TGG + chr1.1:62407068-62407087 MS.gene005321:intron 35.0%
TTTGGATACTTACTGTTCGT+TGG + chr1.1:62409426-62409445 MS.gene005321:CDS 35.0%
! ACAAGTCTAAGATTGTCTCT+CGG - chr1.1:62407557-62407576 None:intergenic 35.0%
! AGACTTGTACCTGTTTTACT+TGG + chr1.1:62407569-62407588 MS.gene005321:CDS 35.0%
! CTCACACTTGCATTTTGTAA+AGG + chr1.1:62408346-62408365 MS.gene005321:intron 35.0%
! GAAAAAAGTGTGTGTACTTG+TGG + chr1.1:62407199-62407218 MS.gene005321:intron 35.0%
! TTTGTAAAGGTACGCGTAAA+AGG + chr1.1:62408359-62408378 MS.gene005321:intron 35.0%
! TTTTATGTAGCCATATGTGG+AGG + chr1.1:62409147-62409166 MS.gene005321:intron 35.0%
!! AATGACTGAAAAAGCACTCA+AGG + chr1.1:62409384-62409403 MS.gene005321:CDS 35.0%
!! AGGTTGGTCTTCAAAACAAA+TGG + chr1.1:62407875-62407894 MS.gene005321:intron 35.0%
!! GGTTGGTCTTCAAAACAAAT+GGG + chr1.1:62407876-62407895 MS.gene005321:intron 35.0%
!!! ACTTTTTTGCCATCAATGTC+CGG - chr1.1:62408969-62408988 None:intergenic 35.0%
!!! ATTTGTTTTGAAGACCAACC+TGG - chr1.1:62407876-62407895 None:intergenic 35.0%
AAAGAAAAGAAGAAACGGCG+AGG + chr1.1:62408873-62408892 MS.gene005321:CDS 40.0%
AACAGTACCATTGGTGAGTT+TGG - chr1.1:62406815-62406834 None:intergenic 40.0%
AGTTGTCTCTGCACTGAAAT+TGG + chr1.1:62407903-62407922 MS.gene005321:CDS 40.0%
ATATCCAAACATGCAGGAAG+TGG - chr1.1:62407067-62407086 None:intergenic 40.0%
ATCCAAATCAACTGGGAAGA+AGG - chr1.1:62409413-62409432 None:intergenic 40.0%
ATTGGACACAACAGTACCAT+TGG - chr1.1:62406824-62406843 None:intergenic 40.0%
CAAGTGTGAGGAGAAAAGAA+AGG - chr1.1:62408336-62408355 None:intergenic 40.0%
CGTGTTTCTTGTGACTTTAG+AGG + chr1.1:62408741-62408760 MS.gene005321:intron 40.0%
CTAGCTAGTGACCTTTATTG+GGG - chr1.1:62407251-62407270 None:intergenic 40.0%
GAGGTCAATATGTGGATAAC+GGG - chr1.1:62407632-62407651 None:intergenic 40.0%
GGAGGAAAGCAAGAAGATAA+AGG + chr1.1:62409165-62409184 MS.gene005321:CDS 40.0%
GGTGCTTATGCTGCAATATT+TGG + chr1.1:62408762-62408781 MS.gene005321:intron 40.0%
GGTGTGCATGCAAATAATAG+AGG - chr1.1:62407729-62407748 None:intergenic 40.0%
TCAGATACCAAACTCACCAA+TGG + chr1.1:62406805-62406824 MS.gene005321:CDS 40.0%
TCCAAATCAACTGGGAAGAA+GGG - chr1.1:62409412-62409431 None:intergenic 40.0%
TGATTCTCTTGAACTTGTCC+AGG + chr1.1:62407855-62407874 MS.gene005321:intron 40.0%
TGGCAAAAAAGTTCTACGCA+TGG + chr1.1:62408977-62408996 MS.gene005321:CDS 40.0%
TGGTGACTTCCAAGTAAAAC+AGG - chr1.1:62407581-62407600 None:intergenic 40.0%
TTCTACAGGTTTCCTGTAAC+CGG + chr1.1:62408003-62408022 MS.gene005321:CDS 40.0%
TTCTTGCTTTCCTCCACATA+TGG - chr1.1:62409160-62409179 None:intergenic 40.0%
TTGGTGAGTTTGGTATCTGA+AGG - chr1.1:62406805-62406824 None:intergenic 40.0%
! CGTTCTCAGAGATTAGTCAT+CGG + chr1.1:62407272-62407291 MS.gene005321:intron 40.0%
! GACTAGCTAGTGACCTTTAT+TGG - chr1.1:62407253-62407272 None:intergenic 40.0%
! GTATCTGAAGGTGATGATGA+TGG - chr1.1:62406793-62406812 None:intergenic 40.0%
! GTGGTGACACAAGTTTTCAT+CGG + chr1.1:62408242-62408261 MS.gene005321:intron 40.0%
!! GAAGAGCACTCAAGATCTTA+AGG + chr1.1:62409258-62409277 MS.gene005321:CDS 40.0%
!! GAGCACTGAAGATCTTAAGA+AGG + chr1.1:62409228-62409247 MS.gene005321:CDS 40.0%
AGACATGAGATTGAACCCAG+AGG - chr1.1:62406899-62406918 None:intergenic 45.0%
CAACAATGCAGCTGTTGAGT+TGG + chr1.1:62409354-62409373 MS.gene005321:CDS 45.0%
GAGCACTCAAGATCTTAAGG+AGG + chr1.1:62409261-62409280 MS.gene005321:CDS 45.0%
GATGTCTGCCACTGATAAAG+AGG + chr1.1:62409019-62409038 MS.gene005321:CDS 45.0%
GTCTCTGCACTGAAATTGGA+AGG + chr1.1:62407907-62407926 MS.gene005321:CDS 45.0%
TACTTGGAAGTCACCAGAAG+AGG + chr1.1:62407585-62407604 MS.gene005321:CDS 45.0%
TCCCTTCTTCCCAGTTGATT+TGG + chr1.1:62409408-62409427 MS.gene005321:CDS 45.0%
TCTCTTGAACTTGTCCAGGT+TGG + chr1.1:62407859-62407878 MS.gene005321:intron 45.0%
TGATATCCACTTTGTCCGGT+AGG - chr1.1:62408903-62408922 None:intergenic 45.0%
! ATTGCCACTTCCTGCATGTT+TGG + chr1.1:62407060-62407079 MS.gene005321:intron 45.0%
!! AATGTGGCAAAGGTGTCCAA+CGG + chr1.1:62408436-62408455 MS.gene005321:CDS 45.0%
!! TGGATAACGGGACAAATACC+TGG - chr1.1:62407620-62407639 None:intergenic 45.0%
!!! ACTGAAGTTTTGGACTCCTC+TGG + chr1.1:62406880-62406899 MS.gene005321:CDS 45.0%
!!! CTGAAGTTTTGGACTCCTCT+GGG + chr1.1:62406881-62406900 MS.gene005321:CDS 45.0%
AGCCAGCAGTCAAGAACCTT+GGG + chr1.1:62408803-62408822 MS.gene005321:CDS 50.0%
AGGAAGCAGAACTCGACTAC+TGG + chr1.1:62408379-62408398 MS.gene005321:CDS 50.0%
CATTTGGCTAGAGTAGCCGT+TGG - chr1.1:62408455-62408474 None:intergenic 50.0%
CTGGTGAAAACCTCCTCTTC+TGG - chr1.1:62407601-62407620 None:intergenic 50.0%
GAAAAGAGGCCTAGTGTGTG+CGG + chr1.1:62409283-62409302 MS.gene005321:CDS 50.0%
GCTCATGATCCGGACATTGA+TGG + chr1.1:62408957-62408976 MS.gene005321:CDS 50.0%
GGCTACTCTAGCCAAATGAC+AGG + chr1.1:62408457-62408476 MS.gene005321:CDS 50.0%
TACGCATGGCTGTTGATGCT+TGG + chr1.1:62408991-62409010 MS.gene005321:CDS 50.0%
TAGTCGTAACCGCACACACT+AGG - chr1.1:62409295-62409314 None:intergenic 50.0%
TATCCACTTTGTCCGGTAGG+AGG - chr1.1:62408900-62408919 None:intergenic 50.0%
TTGGAAGTCACCAGAAGAGG+AGG + chr1.1:62407588-62407607 MS.gene005321:CDS 50.0%
! CAGAAGAGGAGGTTTTCACC+AGG + chr1.1:62407599-62407618 MS.gene005321:CDS 50.0%
!! TTAGATACCTTCGGTGCTGC+CGG - chr1.1:62408025-62408044 None:intergenic 50.0%
CACCCAAGGTTCTTGACTGC+TGG - chr1.1:62408808-62408827 None:intergenic 55.0%
GAGCCAGCAGTCAAGAACCT+TGG + chr1.1:62408802-62408821 MS.gene005321:CDS 55.0%
TCGAGGGGAGACCCCAATAA+AGG + chr1.1:62407237-62407256 MS.gene005321:intron 55.0%
!! GGTGCTTGTAGAAGCACCCA+AGG - chr1.1:62408822-62408841 None:intergenic 55.0%
AAACGGCGAGGTCCTCCTAC+CGG + chr1.1:62408885-62408904 MS.gene005321:CDS 60.0%
GGTCCTCCTACCGGACAAAG+TGG + chr1.1:62408894-62408913 MS.gene005321:CDS 60.0%
! CCTGTAACCGGCAGCACCGA+AGG + chr1.1:62408015-62408034 MS.gene005321:CDS 65.0%
!! CCTTCGGTGCTGCCGGTTAC+AGG - chr1.1:62408018-62408037 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 62406766 62409474 62406766 ID=MS.gene005321
chr1.1 mRNA 62406766 62409474 62406766 ID=MS.gene005321.t1;Parent=MS.gene005321
chr1.1 exon 62406766 62406914 62406766 ID=MS.gene005321.t1.exon1;Parent=MS.gene005321.t1
chr1.1 CDS 62406766 62406914 62406766 ID=cds.MS.gene005321.t1;Parent=MS.gene005321.t1
chr1.1 exon 62407545 62407620 62407545 ID=MS.gene005321.t1.exon2;Parent=MS.gene005321.t1
chr1.1 CDS 62407545 62407620 62407545 ID=cds.MS.gene005321.t1;Parent=MS.gene005321.t1
chr1.1 exon 62407877 62408036 62407877 ID=MS.gene005321.t1.exon3;Parent=MS.gene005321.t1
chr1.1 CDS 62407877 62408036 62407877 ID=cds.MS.gene005321.t1;Parent=MS.gene005321.t1
chr1.1 exon 62408368 62408478 62408368 ID=MS.gene005321.t1.exon4;Parent=MS.gene005321.t1
chr1.1 CDS 62408368 62408478 62408368 ID=cds.MS.gene005321.t1;Parent=MS.gene005321.t1
chr1.1 exon 62408802 62409040 62408802 ID=MS.gene005321.t1.exon5;Parent=MS.gene005321.t1
chr1.1 CDS 62408802 62409040 62408802 ID=cds.MS.gene005321.t1;Parent=MS.gene005321.t1
chr1.1 exon 62409157 62409474 62409157 ID=MS.gene005321.t1.exon6;Parent=MS.gene005321.t1
chr1.1 CDS 62409157 62409474 62409157 ID=cds.MS.gene005321.t1;Parent=MS.gene005321.t1
Gene Sequence

>MS.gene005321

ATGGAAGGCAACAAGACTATGGTTCCATCATCATCACCTTCAGATACCAAACTCACCAATGGTACTGTTGTGTCCAATGATGACTTAGAGATAGAGACCTTCTATGTCAAACTAACTGAAGTTTTGGACTCCTCTGGGTTCAATCTCATGTCTGTTTCTTCAATCAGCTTCTTACTTTCTGTTTTGCTTTTATTTTTTTCGTTTGTATTTGCATGAGTGTTTTTACTTGTGTGCATTATGCTATGTTTGGTTTTACAAAATTCGCATGCAAAAAACGTCAAGCATTTTTTTTTAATTGCCACTTCCTGCATGTTTGGATATTTGGATTCGCTGTAAATTTGTAAAAATAACAGTGACATACTTGATTTTATTCTCCAATTTGTAGTTTTTGCCACAAATTTATCCTCAATCCAAACATGCAATAAAAGAAAAAGAAAAAAGTGTGTGTACTTGTGGATAAAAATATTTGGTTCGAGGGGAGACCCCAATAAAGGTCACTAGCTAGTCGTTCTCAGAGATTAGTCATCGGCAAAGCCACCACAAAAGAAAAATTGAGACATGCATTTAGTCATATTGGTCCTTAAAACTGAAATGTGTTATAATTTATTCCTCTTTAAATGTTTGGTATAAGGTCACTGGAATAGTAGAATGTGTTACAATTTAGAAAGAAGAATACTTGTAATATTATATAAAGTTTTATGAGATTATTTGGATAAAAAAAAATGATGGATAATAAAGGGTATATGACCATGCAACTCTGTTTCAATTTTACATGACAGTTTCAATGTCCGAGAGACAATCTTAGACTTGTACCTGTTTTACTTGGAAGTCACCAGAAGAGGAGGTTTTCACCAGGTATTTGTCCCGTTATCCACATATTGACCTCACATGATTTAATTTGTTCTATTTGTTATATGTTCATTTCTCATTAGTGCATGTGTATATATTTTATGCATGTAGCCTCTATTATTTGCATGCACACCAAGTCTATCCATCAAATATGTTGTGTCTATATAATACAAATTAGTCAATAGAGGCTTTTAATTAAGTAATTATCGTATGAATTGTTCAAATTTAATATATTGTAATTGATTCTCTTGAACTTGTCCAGGTTGGTCTTCAAAACAAATGGGCTGAAGTTGTCTCTGCACTGAAATTGGAAGGCAGCAATGCAATGTTATCTGCTCAAGTTGAAAAGATCTATGCAACACTTCTATACAAATTTGAGAAATTGTACTTCTACAGGTTTCCTGTAACCGGCAGCACCGAAGGTATCTAAGAGATCAATTTGTGGTTATTTTACGTAGATATCGATCTTGTTTCATTCCGGTACTTCACTTATATGCAGTATGCTTGATATGTAAATGTTTGGTAGATTATACATAATATGATTTTAATTTATGACTAGTAATAGAAAAATGCCTTGTAAAAACATTGGTCTAAGAAAATGTATTGGATGTGAAATTAAATCACTCGGTGGTGACACAAGTTTTCATCGGATCATTGTATGATGTTTTTATTACGTTTTTGTTTTAGGATGAAAATGTGTTCTTTAACCTTAAAGTCTCCTTTCTTTTCTCCTCACACTTGCATTTTGTAAAGGTACGCGTAAAAGGAAGCAGAACTCGACTACTGGTTTATCTCGATTAATGGCTCATGAAGATAATCTGAATGTGGCAAAGGTGTCCAACGGCTACTCTAGCCAAATGACAGGTTATAAGGATTTAGAATATTTATGCATTGAAAATTTGATTCACTGATTGAATGCAAAAGGTTGCACTCAAGTACTAATGAATTAACTAGCTAACTAGCTGAATCAAAACTAATATCACAACAGAACACAATTTATCAACTAATCATGATAACTGAGATTACTAAAGTTCCAAAACAGGTTTGTTACTTTATGTACTTATTAGCTGCAATGTGGACATTTGTTTTCTTTAGTAACTCAAGTGATTTGTTAAATGAGTTTAAACGTGTTTCTTGTGACTTTAGAGGTGCTTATGCTGCAATATTTGGATTCTTATTTAAAGCAGGAGCCAGCAGTCAAGAACCTTGGGTGCTTCTACAAGCACCCTCAAATGACAAAGAAAAGAAGAAACAAGACAAAGAAAAGAAGAAACGGCGAGGTCCTCCTACCGGACAAAGTGGATATCAAATATTTCTCAAGCATGAATGTGCTCGATTAAAAGCTCATGATCCGGACATTGATGGCAAAAAAGTTCTACGCATGGCTGTTGATGCTTGGCAGAAGATGTCTGCCACTGATAAAGAGGTATGATAAATAAAATTGACATAATATCGATCAAATGTCGAACAATTTATATTTTTGCAATTAGCCTCTACTTTGTTTGTTTGAAATTTTTCATTTATAATTACTTTTTTATGTAGCCATATGTGGAGGAAAGCAAGAAGATAAAGGAAAAAAAGAAGGAAGCAGTGATCATTGACAATAAACAGATGAGCACTGAAGATCTTAAGAAGGATGAAAAGAAGAGCACTCAAGATCTTAAGGAGGAAAAGAGGCCTAGTGTGTGCGGTTACGACTACTGTGCAACTTCGCAACCACTACCAAACTATTCTTCTGTCAACAATGCAGCTGTTGAGTTGGCTTTGAAAATGACTGAAAAAGCACTCAAGGATCCCTTCTTCCCAGTTGATTTGGATACTTACTGTTCGTTGGATATGTTAAATGGGAAATCCAAATGA
Protein sequence

>MS.gene005321.t1

MEGNKTMVPSSSPSDTKLTNGTVVSNDDLEIETFYVKLTEVLDSSGFNLIFNVRETILDLYLFYLEVTRRGGFHQVGLQNKWAEVVSALKLEGSNAMLSAQVEKIYATLLYKFEKLYFYRFPVTGSTEGTRKRKQNSTTGLSRLMAHEDNLNVAKVSNGYSSQMTGASSQEPWVLLQAPSNDKEKKKQDKEKKKRRGPPTGQSGYQIFLKHECARLKAHDPDIDGKKVLRMAVDAWQKMSATDKEPYVEESKKIKEKKKEAVIIDNKQMSTEDLKKDEKKSTQDLKEEKRPSVCGYDYCATSQPLPNYSSVNNAAVELALKMTEKALKDPFFPVDLDTYCSLDMLNGKSK