AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005324

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005324.t1 MTR_5g015480 88.649 185 21 0 1 185 1 185 2.82e-110 333
MS.gene005324.t1 MTR_0402s0040 67.317 205 62 2 36 236 2 205 9.44e-86 278
MS.gene005324.t1 MTR_0402s0040 51.056 284 69 3 394 677 200 413 3.83e-74 247
MS.gene005324.t1 MTR_6g017200 70.248 121 32 1 36 152 34 154 2.36e-49 169
MS.gene005324.t1 MTR_2g070590 68.908 119 37 0 40 158 1 119 4.85e-49 167
MS.gene005324.t1 MTR_7g113010 56.250 160 52 2 26 181 423 568 9.30e-45 169
MS.gene005324.t1 MTR_1g086270 60.000 125 34 2 329 453 1 109 2.28e-43 152
MS.gene005324.t1 MTR_1g057920 63.415 123 41 1 27 145 1 123 4.76e-43 151
MS.gene005324.t1 MTR_0137s0040 68.182 88 24 1 25 108 1 88 7.27e-34 125
MS.gene005324.t1 MTR_6g051425 29.252 294 164 6 63 315 24 314 1.94e-32 129
MS.gene005324.t1 MTR_1g070355 30.142 282 157 8 23 271 37 311 1.32e-28 121
MS.gene005324.t1 MTR_0558s0020 28.340 247 136 4 70 275 50 296 7.11e-27 111
MS.gene005324.t1 MTR_7g074790 42.442 172 77 5 56 226 10 160 2.31e-26 108
MS.gene005324.t1 MTR_1g050985 78.333 60 13 0 126 185 39 98 7.49e-26 102
MS.gene005324.t1 MTR_8g446020 29.508 183 106 1 116 275 74 256 3.70e-24 102
MS.gene005324.t1 MTR_4g102720 35.119 168 73 3 56 223 7 138 4.06e-23 99.0
MS.gene005324.t1 MTR_0291s0060 63.014 73 23 1 38 106 13 85 1.80e-22 92.4
MS.gene005324.t1 MTR_6g090505 30.769 182 103 1 116 274 49 230 1.58e-21 97.4
MS.gene005324.t1 MTR_5g076070 28.216 241 149 5 46 263 43 282 4.43e-21 94.4
MS.gene005324.t1 MTR_4g067290 46.296 108 52 3 56 161 56 159 1.09e-20 92.0
MS.gene005324.t1 MTR_4g067290 46.296 108 52 3 56 161 56 159 1.10e-20 92.0
MS.gene005324.t1 MTR_4g067290 46.296 108 52 3 56 161 74 177 1.57e-20 92.0
MS.gene005324.t1 MTR_4g067290 46.296 108 52 3 56 161 76 179 1.61e-20 92.0
MS.gene005324.t1 MTR_4g067290 46.296 108 52 3 56 161 76 179 1.67e-20 92.0
MS.gene005324.t1 MTR_4g035820 30.418 263 148 9 48 279 66 324 3.21e-20 95.9
MS.gene005324.t1 MTR_4g067290 46.296 108 52 3 56 161 116 219 4.07e-20 92.0
MS.gene005324.t1 MTR_2g098920 41.228 114 61 3 56 165 80 191 5.80e-19 87.8
MS.gene005324.t1 MTR_6g022080 31.818 154 102 2 62 213 91 243 2.15e-18 85.9
MS.gene005324.t1 MTR_1g091013 67.857 56 18 0 69 124 14 69 9.31e-18 78.2
MS.gene005324.t1 MTR_6g022070 28.736 174 119 1 329 502 1 169 6.46e-16 80.1
MS.gene005324.t1 MTR_7g061980 40.336 119 67 3 50 167 4 119 7.95e-16 74.7
MS.gene005324.t1 MTR_7g446520 26.531 245 140 4 59 280 91 318 9.45e-16 79.3
MS.gene005324.t1 MTR_1g083640 25.221 226 128 4 62 246 57 282 3.16e-15 77.0
MS.gene005324.t1 MTR_2g014100 24.607 191 126 3 62 234 50 240 5.72e-14 72.8
MS.gene005324.t1 MTR_3g006370 39.048 105 62 2 56 159 50 153 1.11e-13 71.6
MS.gene005324.t1 MTR_2g099430 40.909 88 50 1 80 167 27 112 1.51e-13 67.8
MS.gene005324.t1 MTR_5g045210 38.202 89 55 0 566 654 20 108 2.18e-13 69.7
MS.gene005324.t1 MTR_1g019850 30.488 164 104 3 34 194 45 201 5.37e-13 69.7
MS.gene005324.t1 MTR_5g039760 33.333 180 91 6 60 237 14 166 5.79e-13 68.6
MS.gene005324.t1 MTR_1g008290 30.345 145 88 3 108 244 3 142 1.13e-12 66.2
MS.gene005324.t1 MTR_2g098880 34.351 131 80 4 56 182 73 201 2.23e-12 68.2
MS.gene005324.t1 MTR_2g098880 34.351 131 80 4 56 182 61 189 2.25e-12 68.2
MS.gene005324.t1 MTR_0337s0040 27.869 122 88 0 116 237 75 196 1.35e-11 65.1
MS.gene005324.t1 MTR_0337s0060 28.000 175 83 4 425 589 21 162 1.97e-11 65.1
MS.gene005324.t1 MTR_7g446480 30.120 166 92 2 141 283 149 313 6.30e-11 64.7
MS.gene005324.t1 MTR_1g055240 26.891 119 87 0 116 234 25 143 7.01e-11 61.6
MS.gene005324.t1 MTR_7g446510 28.571 175 101 2 132 283 155 328 9.10e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005324.t1 AT4G38180 34.336 565 337 7 56 589 73 634 1.46e-108 347
MS.gene005324.t1 AT4G15090 30.769 624 396 8 56 651 51 666 6.90e-94 309
MS.gene005324.t1 AT4G15090 30.769 624 396 8 56 651 51 666 6.90e-94 309
MS.gene005324.t1 AT4G15090 30.769 624 396 8 56 651 51 666 6.90e-94 309
MS.gene005324.t1 AT4G15090 30.769 624 396 8 56 651 51 666 6.90e-94 309
MS.gene005324.t1 AT4G15090 30.769 624 396 8 56 651 51 666 6.90e-94 309
MS.gene005324.t1 AT3G22170 31.773 598 379 11 56 634 70 657 4.68e-90 299
MS.gene005324.t1 AT3G22170 31.773 598 379 11 56 634 70 657 4.68e-90 299
MS.gene005324.t1 AT1G76320 31.115 601 371 14 60 634 1 584 8.36e-83 275
MS.gene005324.t1 AT1G76320 31.115 601 371 14 60 634 1 584 1.73e-82 276
MS.gene005324.t1 AT1G76320 31.115 601 371 14 60 634 1 584 1.86e-82 276
MS.gene005324.t1 AT1G76320 31.115 601 371 14 60 634 1 584 1.86e-82 276
MS.gene005324.t1 AT2G27110 30.167 600 371 13 56 634 49 621 5.90e-77 263
MS.gene005324.t1 AT2G27110 30.167 600 371 13 56 634 49 621 5.90e-77 263
MS.gene005324.t1 AT2G32250 27.814 622 410 10 60 669 42 636 2.03e-73 252
MS.gene005324.t1 AT2G32250 27.814 622 410 10 60 669 1 595 2.17e-73 252
MS.gene005324.t1 AT2G32250 27.814 622 410 10 60 669 1 595 2.43e-73 252
MS.gene005324.t1 AT2G32250 27.814 622 410 10 60 669 42 636 4.24e-73 252
MS.gene005324.t1 AT2G32250 27.814 622 410 10 60 669 42 636 4.24e-73 252
MS.gene005324.t1 AT2G32250 27.814 622 410 10 60 669 42 636 4.24e-73 252
MS.gene005324.t1 AT2G32250 27.814 622 410 10 60 669 42 636 4.74e-73 252
MS.gene005324.t1 AT2G32250 27.814 622 410 10 60 669 42 636 4.74e-73 252
MS.gene005324.t1 AT1G10240 28.295 569 372 11 49 586 41 604 5.23e-68 236
MS.gene005324.t1 AT1G10240 28.295 569 372 11 49 586 41 604 5.23e-68 236
MS.gene005324.t1 AT5G18960 29.725 582 356 15 44 607 200 746 8.80e-68 237
MS.gene005324.t1 AT5G18960 33.051 118 65 2 41 158 28 131 2.39e-11 67.8
MS.gene005324.t1 AT5G18960 29.725 582 356 15 44 607 200 746 8.80e-68 237
MS.gene005324.t1 AT5G18960 33.051 118 65 2 41 158 28 131 2.39e-11 67.8
MS.gene005324.t1 AT3G06250 28.028 578 363 12 47 607 181 722 3.68e-67 235
MS.gene005324.t1 AT3G06250 28.028 578 363 12 47 607 181 722 3.68e-67 235
MS.gene005324.t1 AT3G06250 28.028 578 363 12 47 607 181 722 3.68e-67 235
MS.gene005324.t1 AT2G27110 31.707 410 267 6 231 634 74 476 6.89e-65 228
MS.gene005324.t1 AT4G19990 28.105 612 397 11 61 650 26 616 1.52e-58 211
MS.gene005324.t1 AT4G19990 28.105 612 397 11 61 650 26 616 1.84e-58 211
MS.gene005324.t1 AT4G19990 28.105 612 397 11 61 650 26 616 1.84e-58 211
MS.gene005324.t1 AT4G19990 28.105 612 397 11 61 650 40 630 1.98e-58 211
MS.gene005324.t1 AT4G19990 27.941 612 398 10 61 650 26 616 3.86e-58 210
MS.gene005324.t1 AT4G19990 27.941 612 398 10 61 650 26 616 3.86e-58 210
MS.gene005324.t1 AT4G38170 29.040 396 266 7 205 590 10 400 2.85e-56 201
MS.gene005324.t1 AT5G28530 24.760 626 416 12 56 637 55 669 1.59e-55 201
MS.gene005324.t1 AT5G28530 25.000 632 403 13 56 637 55 665 1.85e-55 201
MS.gene005324.t1 AT5G28530 25.000 632 403 13 56 637 55 665 1.85e-55 201
MS.gene005324.t1 AT1G80010 27.787 583 356 13 56 589 95 661 3.10e-54 198
MS.gene005324.t1 AT1G52520 27.660 564 368 16 54 587 83 636 4.96e-53 194
MS.gene005324.t1 AT4G19990 27.336 578 377 10 95 650 3 559 1.16e-50 187
MS.gene005324.t1 AT5G28530 24.558 566 373 13 56 609 55 578 1.54e-42 163
MS.gene005324.t1 AT5G28530 24.825 572 360 14 56 609 55 574 2.14e-42 163
MS.gene005324.t1 AT3G59470 46.226 106 55 2 56 161 70 173 2.46e-21 94.4
MS.gene005324.t1 AT3G59470 46.226 106 55 2 56 161 70 173 2.46e-21 94.4
MS.gene005324.t1 AT3G59470 46.226 106 55 2 56 161 82 185 3.54e-21 94.4
MS.gene005324.t1 AT3G07500 38.760 129 76 2 34 160 9 136 2.22e-15 76.3
MS.gene005324.t1 AT2G43280 37.500 128 71 4 56 181 22 142 7.11e-14 71.6
MS.gene005324.t1 AT4G12850 32.540 126 78 3 56 181 11 129 2.31e-12 65.5
MS.gene005324.t1 AT2G43280 40.659 91 50 3 71 159 2 90 1.37e-11 63.9
MS.gene005324.t1 AT4G12850 34.951 103 63 2 56 158 11 109 2.81e-11 62.4
MS.gene005324.t1 AT4G12850 34.951 103 63 2 56 158 11 109 9.02e-11 62.0

Find 113 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAAATAACAAACATATTT+TGG 0.063618 1.1:-62466336 MS.gene005324:CDS
CACAGACAAACAATTGTTTA+TGG 0.199392 1.1:-62466196 MS.gene005324:CDS
TTGTTTGTCTGTGGCTATTA+AGG 0.202792 1.1:+62466206 None:intergenic
ATTCATCAGCCAATACAATT+TGG 0.204201 1.1:+62466572 None:intergenic
GCACAAATGATAAATGATTA+TGG 0.212778 1.1:-62466307 MS.gene005324:CDS
CACAATCCATGAAATGTTTC+TGG 0.237682 1.1:+62466031 None:intergenic
GAAATTTGCTTCGCACTAAA+AGG 0.249768 1.1:-62466462 MS.gene005324:CDS
GGTTGGGATGTAATCAAAAT+TGG 0.250331 1.1:-62465277 MS.gene005324:CDS
GATTGTGCACTTGGCATATA+AGG 0.257928 1.1:-62466015 MS.gene005324:CDS
TGGACATAAAAGCTCATTTC+AGG 0.266817 1.1:-62465235 MS.gene005324:CDS
ACAAATTCATGAAAATCTTT+TGG 0.298226 1.1:+62466538 None:intergenic
ATTGTATTGGCTGATGAATC+TGG 0.322531 1.1:-62466568 MS.gene005324:CDS
ATGAGACTCTTCATAAACTT+TGG 0.330181 1.1:+62465130 None:intergenic
CATTTCATGGATTGTGCACT+TGG 0.334162 1.1:-62466024 MS.gene005324:CDS
TAGTGCGAAGCAAATTTCTA+TGG 0.349224 1.1:+62466467 None:intergenic
GACATTCCTCATATTCATCT+TGG 0.362108 1.1:+62465531 None:intergenic
TCTTGGAGTGTAAACATTGC+CGG 0.362313 1.1:+62465568 None:intergenic
TGAGACTCTTCATAAACTTT+GGG 0.365433 1.1:+62465131 None:intergenic
ACAGACAAACAATTGTTTAT+GGG 0.367661 1.1:-62466195 MS.gene005324:CDS
TATAGTAGGAAATGTGGTTT+TGG 0.369598 1.1:-62466886 MS.gene005324:CDS
TTCATCAGCCAATACAATTT+GGG 0.375471 1.1:+62466573 None:intergenic
GAAAGGGGAACATATTGAAT+TGG 0.379587 1.1:-62465255 MS.gene005324:CDS
TCAAATAACCATTGAAAAGA+AGG 0.380370 1.1:+62466145 None:intergenic
GGTGAATGATATCGTGCAAC+AGG 0.381657 1.1:-62466992 MS.gene005324:CDS
TCACCGTTCATCACCACCTC+TGG 0.383906 1.1:-62467207 MS.gene005324:CDS
CCCACCCATTGCTTTGAGAA+AGG 0.392844 1.1:+62466120 None:intergenic
ATTTCACTTGATCGGAAGAT+TGG 0.399360 1.1:-62466703 MS.gene005324:CDS
GTCATGCCAGAAACATTTCA+TGG 0.400517 1.1:-62466037 MS.gene005324:CDS
GACACCGCGTCTACCCTCTT+TGG 0.402002 1.1:+62466795 None:intergenic
CACAAATGATAAATGATTAT+GGG 0.407470 1.1:-62466306 MS.gene005324:CDS
ATAGATGATTCACATGCCTT+AGG 0.411459 1.1:+62465984 None:intergenic
TAATGTACCACACTTGGAAA+TGG 0.430430 1.1:-62466950 MS.gene005324:CDS
TACACCTTCAACCATTGGAT+TGG 0.436578 1.1:+62467023 None:intergenic
AACAGGATGTTTAGCAGGTA+TGG 0.436611 1.1:-62466728 MS.gene005324:CDS
ATCATTTATCATTTGTGCAT+CGG 0.444064 1.1:+62466312 None:intergenic
ACGTGTGTCGTTTCTACCAT+TGG 0.448381 1.1:+62467248 None:intergenic
TGTTTAAGAAATTTGAAAGA+AGG 0.449252 1.1:-62465500 MS.gene005324:CDS
TAGATGATTCACATGCCTTA+GGG 0.455627 1.1:+62465985 None:intergenic
GAAACCTTTCTCAAAGCAAT+GGG 0.457265 1.1:-62466124 MS.gene005324:CDS
TGCATCTTGATCTGTTAGAA+TGG 0.469730 1.1:+62466084 None:intergenic
GAATTTGTGTCAAAACAAGC+TGG 0.470933 1.1:-62466523 MS.gene005324:CDS
GGAGTTTGAGTCTGAGGCCT+TGG 0.475074 1.1:-62466929 MS.gene005324:CDS
TGAAACCTTTCTCAAAGCAA+TGG 0.476564 1.1:-62466125 MS.gene005324:CDS
GTTTAAGAAATTTGAAAGAA+GGG 0.482494 1.1:-62465499 MS.gene005324:CDS
ATAAATGATTATGGGTACTT+TGG 0.482723 1.1:-62466298 MS.gene005324:CDS
GGAATTCCAAGATGAATATG+AGG 0.483458 1.1:-62465537 MS.gene005324:CDS
AATAAGAATTATCGTCCGTT+GGG 0.484650 1.1:-62466241 MS.gene005324:CDS
CTTCACCATACCTTAGTGAA+TGG 0.485222 1.1:+62466437 None:intergenic
CAAATAACCATTGAAAAGAA+GGG 0.495588 1.1:+62466146 None:intergenic
ACAAGCATCCCAAATTGTAT+TGG 0.495869 1.1:-62466581 MS.gene005324:CDS
GATGTAATCAAAATTGGAAA+GGG 0.496181 1.1:-62465271 MS.gene005324:CDS
TCTATGGATGGAGAGTCAAT+TGG 0.511024 1.1:-62465077 MS.gene005324:CDS
ACTAGAACAGGATGTTTAGC+AGG 0.515632 1.1:-62466733 MS.gene005324:CDS
CACCGTTCATCACCACCTCT+GGG 0.518896 1.1:-62467206 MS.gene005324:CDS
TTGCCGGCTTGTACAAGCAT+AGG 0.519591 1.1:+62465584 None:intergenic
ATCCCTATGCTTGTACAAGC+CGG 0.521377 1.1:-62465587 MS.gene005324:CDS
ATTGTGCACTTGGCATATAA+GGG 0.522000 1.1:-62466014 MS.gene005324:CDS
ATCATATAAACATACTCAGC+TGG 0.522562 1.1:+62466631 None:intergenic
GATAGTAGTAGCGAATCATA+TGG 0.523742 1.1:-62465438 MS.gene005324:CDS
TTGGACAAGAGATGCAAGGT+TGG 0.524873 1.1:-62465294 MS.gene005324:CDS
CATACTCAGCTGGCAGAAGA+AGG 0.525063 1.1:+62466641 None:intergenic
AAATAAGAATTATCGTCCGT+TGG 0.526037 1.1:-62466242 MS.gene005324:CDS
TGGACAAGAGATGCAAGGTT+GGG 0.530700 1.1:-62465293 MS.gene005324:CDS
ACATCCAATCCAATGGTTGA+AGG 0.531051 1.1:-62467027 MS.gene005324:CDS
GATTCAAGGGAAAATGGGCA+TGG 0.535225 1.1:-62465820 MS.gene005324:CDS
GCTCGAGCAGAAACTAGAAC+AGG 0.536024 1.1:-62466745 MS.gene005324:CDS
GTATGGTTATTTCACTTGAT+CGG 0.538559 1.1:-62466711 MS.gene005324:CDS
TCAAGATGCAGCAATGGCTA+AGG 0.541612 1.1:-62466071 MS.gene005324:CDS
CAGACAAACAATTGTTTATG+GGG 0.542243 1.1:-62466194 MS.gene005324:CDS
ACGTGATTACTTAACAAAAG+AGG 0.543276 1.1:-62466767 MS.gene005324:CDS
AATTTGTGTCAAAACAAGCT+GGG 0.543407 1.1:-62466522 MS.gene005324:CDS
AGCAATATATACTAAAACGT+TGG 0.545983 1.1:-62465313 MS.gene005324:CDS
ATAATGTTTCTAAAGACTCA+TGG 0.548211 1.1:-62465859 MS.gene005324:CDS
GGAAATGGAGTTTGAGTCTG+AGG 0.549669 1.1:-62466935 MS.gene005324:CDS
AATGAGTATAGTAGGAAATG+TGG 0.549866 1.1:-62466892 MS.gene005324:CDS
ACCTTTCTCAAAGCAATGGG+TGG 0.552305 1.1:-62466121 MS.gene005324:CDS
GGATGTAATCAAAATTGGAA+AGG 0.552421 1.1:-62465272 MS.gene005324:CDS
TCGGAAGATTGGAAAATACA+AGG 0.556421 1.1:-62466692 MS.gene005324:CDS
AGGAACCATTCACTAAGGTA+TGG 0.558908 1.1:-62466442 MS.gene005324:CDS
TGTTAGTAATGTACCACACT+TGG 0.560348 1.1:-62466956 MS.gene005324:CDS
TTGCTCAATTTAACTGCTCG+AGG 0.564353 1.1:+62465191 None:intergenic
ATCTTACCGGTGCCCAGAGG+TGG 0.565589 1.1:+62467194 None:intergenic
ATTATAAAATTCATATGCCA+AGG 0.568982 1.1:+62466912 None:intergenic
AACAGATCAAGATGCAGCAA+TGG 0.573110 1.1:-62466077 MS.gene005324:CDS
ATTCTTATTTGTCTTGAATG+TGG 0.574010 1.1:+62466255 None:intergenic
TGCCGGCTTGTACAAGCATA+GGG 0.574909 1.1:+62465585 None:intergenic
TCATCACCACCTCTGGGCAC+CGG 0.577571 1.1:-62467200 MS.gene005324:intron
GTGCGAAAGACTTTCACTGC+AGG 0.578283 1.1:-62465791 MS.gene005324:CDS
TGTCGAAAGTTTGAGACACA+TGG 0.578537 1.1:-62465395 MS.gene005324:CDS
CATAAACAATTGTTTGTCTG+TGG 0.584673 1.1:+62466197 None:intergenic
ATTCAAGGGAAAATGGGCAT+GGG 0.587348 1.1:-62465819 MS.gene005324:CDS
TGCCCAGAGGTGGTGATGAA+CGG 0.588261 1.1:+62467204 None:intergenic
CTAAAAGGAACCATTCACTA+AGG 0.588493 1.1:-62466447 MS.gene005324:CDS
AAAGAGTTGTCAACGGAAAG+CGG 0.589356 1.1:-62465673 MS.gene005324:CDS
AAAATTTGTGTGCTCCAAAG+AGG 0.589431 1.1:-62466809 MS.gene005324:CDS
CACTTTGAAAGAGTTGTCAA+CGG 0.610932 1.1:-62465680 MS.gene005324:CDS
CCTTTCTCAAAGCAATGGGT+GGG 0.612770 1.1:-62466120 MS.gene005324:CDS
ATGTAATCAAAATTGGAAAG+GGG 0.613707 1.1:-62465270 MS.gene005324:CDS
ATGCATTGATCTACACGAAG+AGG 0.614015 1.1:-62465924 MS.gene005324:CDS
GAATATTACATCCAATCCAA+TGG 0.614280 1.1:-62467034 MS.gene005324:CDS
AAATTTGTGTGCTCCAAAGA+GGG 0.614397 1.1:-62466808 MS.gene005324:CDS
ATTTGTGTCAAAACAAGCTG+GGG 0.616608 1.1:-62466521 MS.gene005324:CDS
CAAGGTTATTGACTTTGTAG+CGG 0.620198 1.1:-62466674 MS.gene005324:CDS
AACGTTGGACAAGAGATGCA+AGG 0.629157 1.1:-62465298 MS.gene005324:CDS
TACAAATTATGATAACAGCA+AGG 0.637062 1.1:-62465465 MS.gene005324:CDS
TCAAACTCCATTTCCAAGTG+TGG 0.642402 1.1:+62466943 None:intergenic
ATATAAGGGAAAATGCCCTA+AGG 0.647376 1.1:-62466000 MS.gene005324:CDS
AGGCTAACAAATACTCCCAA+CGG 0.653067 1.1:+62466226 None:intergenic
TGCTCCAAAGAGGGTAGACG+CGG 0.660484 1.1:-62466799 MS.gene005324:CDS
GTGAATGATATCGTGCAACA+GGG 0.667513 1.1:-62466991 MS.gene005324:CDS
ATTATGATAACAGCAAGGAG+CGG 0.694152 1.1:-62465460 MS.gene005324:CDS
TTTGTGTCAAAACAAGCTGG+GGG 0.697793 1.1:-62466520 MS.gene005324:CDS
AAAATCTTACCGGTGCCCAG+AGG 0.742952 1.1:+62467191 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTATAATGAGTATAGT+AGG - chr1.1:62465376-62465395 MS.gene005324:CDS 15.0%
!! ATCAATTGCAAATGAAAAAA+AGG - chr1.1:62467240-62467259 MS.gene005324:CDS 20.0%
!! ATTATAAAATTCATATGCCA+AGG + chr1.1:62465367-62465386 None:intergenic 20.0%
!! CACAAATGATAAATGATTAT+GGG - chr1.1:62465970-62465989 MS.gene005324:CDS 20.0%
!! GTTTAAGAAATTTGAAAGAA+GGG - chr1.1:62466777-62466796 MS.gene005324:CDS 20.0%
!! TATTCACATTATCTAGAAAA+TGG + chr1.1:62465227-62465246 None:intergenic 20.0%
!! TGTTTAAGAAATTTGAAAGA+AGG - chr1.1:62466776-62466795 MS.gene005324:CDS 20.0%
!!! ACAAATTCATGAAAATCTTT+TGG + chr1.1:62465741-62465760 None:intergenic 20.0%
!!! AGCAAATAACAAACATATTT+TGG - chr1.1:62465940-62465959 MS.gene005324:CDS 20.0%
!!! ATCTTTTTCATTTTCAATCT+TGG + chr1.1:62466671-62466690 None:intergenic 20.0%
!!! CATAATATTTTTCGATTCAA+GGG - chr1.1:62466443-62466462 MS.gene005324:CDS 20.0%
!!! CATGAATATTTGAAAGTATT+TGG - chr1.1:62465139-62465158 MS.gene005324:CDS 20.0%
!!! GCAAATAACAAACATATTTT+GGG - chr1.1:62465941-62465960 MS.gene005324:CDS 20.0%
! ACAGACAAACAATTGTTTAT+GGG - chr1.1:62466081-62466100 MS.gene005324:CDS 25.0%
! AGCAATATATACTAAAACGT+TGG - chr1.1:62466963-62466982 MS.gene005324:CDS 25.0%
! ATAAATGATTATGGGTACTT+TGG - chr1.1:62465978-62465997 MS.gene005324:CDS 25.0%
! ATCATTTATCATTTGTGCAT+CGG + chr1.1:62465967-62465986 None:intergenic 25.0%
! ATGTAATCAAAATTGGAAAG+GGG - chr1.1:62467006-62467025 MS.gene005324:CDS 25.0%
! CAAATAACCATTGAAAAGAA+GGG + chr1.1:62466133-62466152 None:intergenic 25.0%
! CCTTGAATCGAAAAATATTA+TGG + chr1.1:62466445-62466464 None:intergenic 25.0%
! GATGTAATCAAAATTGGAAA+GGG - chr1.1:62467005-62467024 MS.gene005324:CDS 25.0%
! GCAAATAAAAGCAAAAAAGA+TGG - chr1.1:62465429-62465448 MS.gene005324:CDS 25.0%
! GCACAAATGATAAATGATTA+TGG - chr1.1:62465969-62465988 MS.gene005324:CDS 25.0%
! TACAAATTATGATAACAGCA+AGG - chr1.1:62466811-62466830 MS.gene005324:CDS 25.0%
! TCAAATAACCATTGAAAAGA+AGG + chr1.1:62466134-62466153 None:intergenic 25.0%
! TGAAACTCATAAAAGAATGA+AGG + chr1.1:62465906-62465925 None:intergenic 25.0%
!! ATAATGTTTCTAAAGACTCA+TGG - chr1.1:62466417-62466436 MS.gene005324:intron 25.0%
!! ATTCTTATTTGTCTTGAATG+TGG + chr1.1:62466024-62466043 None:intergenic 25.0%
!! ATTCTTTTATGAGTTTCAGA+TGG - chr1.1:62465908-62465927 MS.gene005324:CDS 25.0%
!!! AAGAAGAATTTTTTAGTGGA+TGG - chr1.1:62466375-62466394 MS.gene005324:intron 25.0%
!!! AGAAGAATTTTTTAGTGGAT+GGG - chr1.1:62466376-62466395 MS.gene005324:intron 25.0%
!!! CCATAATATTTTTCGATTCA+AGG - chr1.1:62466442-62466461 MS.gene005324:CDS 25.0%
!!! TTTTATTTGCATATTCTCGA+CGG + chr1.1:62465419-62465438 None:intergenic 25.0%
AAATAAGAATTATCGTCCGT+TGG - chr1.1:62466034-62466053 MS.gene005324:CDS 30.0%
AATAAGAATTATCGTCCGTT+GGG - chr1.1:62466035-62466054 MS.gene005324:CDS 30.0%
AATGAGTATAGTAGGAAATG+TGG - chr1.1:62465384-62465403 MS.gene005324:CDS 30.0%
AATTTGTGTCAAAACAAGCT+GGG - chr1.1:62465754-62465773 MS.gene005324:CDS 30.0%
ACGTGATTACTTAACAAAAG+AGG - chr1.1:62465509-62465528 MS.gene005324:CDS 30.0%
ATCATATAAACATACTCAGC+TGG + chr1.1:62465648-62465667 None:intergenic 30.0%
ATGAGACTCTTCATAAACTT+TGG + chr1.1:62467149-62467168 None:intergenic 30.0%
ATTCATCAGCCAATACAATT+TGG + chr1.1:62465707-62465726 None:intergenic 30.0%
CACAGACAAACAATTGTTTA+TGG - chr1.1:62466080-62466099 MS.gene005324:CDS 30.0%
CAGACAAACAATTGTTTATG+GGG - chr1.1:62466082-62466101 MS.gene005324:CDS 30.0%
CATAAACAATTGTTTGTCTG+TGG + chr1.1:62466082-62466101 None:intergenic 30.0%
GAATATTACATCCAATCCAA+TGG - chr1.1:62465242-62465261 MS.gene005324:CDS 30.0%
GACAAAGAACAAAATCTTAC+CGG + chr1.1:62465098-62465117 None:intergenic 30.0%
GGATGTAATCAAAATTGGAA+AGG - chr1.1:62467004-62467023 MS.gene005324:CDS 30.0%
TGAGACTCTTCATAAACTTT+GGG + chr1.1:62467148-62467167 None:intergenic 30.0%
TGGAATTCCTCAAAAATTCT+TGG + chr1.1:62466728-62466747 None:intergenic 30.0%
TTCATCAGCCAATACAATTT+GGG + chr1.1:62465706-62465725 None:intergenic 30.0%
! AAACAATCCCTTCTTTTCAA+TGG - chr1.1:62466123-62466142 MS.gene005324:CDS 30.0%
! GTATGGTTATTTCACTTGAT+CGG - chr1.1:62465565-62465584 MS.gene005324:CDS 30.0%
! TATAGTAGGAAATGTGGTTT+TGG - chr1.1:62465390-62465409 MS.gene005324:CDS 30.0%
! TATGCTGACTAAAAAGTCTA+TGG - chr1.1:62467183-62467202 MS.gene005324:intron 30.0%
! TGATCTGTTAGAATGGTTTT+AGG + chr1.1:62466188-62466207 None:intergenic 30.0%
! TTACACTCCAAGAATTTTTG+AGG - chr1.1:62466718-62466737 MS.gene005324:CDS 30.0%
! TTTTCGATTCAAGGGAAAAT+GGG - chr1.1:62466451-62466470 MS.gene005324:CDS 30.0%
!! CGTTTTAGTATATATTGCTG+AGG + chr1.1:62466962-62466981 None:intergenic 30.0%
!! TTTTTCGATTCAAGGGAAAA+TGG - chr1.1:62466450-62466469 MS.gene005324:CDS 30.0%
!!! GAGGAAGAAGAATTTTTTAG+TGG - chr1.1:62466371-62466390 MS.gene005324:intron 30.0%
AAAATTTGTGTGCTCCAAAG+AGG - chr1.1:62465467-62465486 MS.gene005324:CDS 35.0%
AAATTTGTGTGCTCCAAAGA+GGG - chr1.1:62465468-62465487 MS.gene005324:CDS 35.0%
ACAAGCATCCCAAATTGTAT+TGG - chr1.1:62465695-62465714 MS.gene005324:CDS 35.0%
ATAGATGATTCACATGCCTT+AGG + chr1.1:62466295-62466314 None:intergenic 35.0%
ATATAAGGGAAAATGCCCTA+AGG - chr1.1:62466276-62466295 MS.gene005324:CDS 35.0%
ATTATGATAACAGCAAGGAG+CGG - chr1.1:62466816-62466835 MS.gene005324:CDS 35.0%
ATTGTGCACTTGGCATATAA+GGG - chr1.1:62466262-62466281 MS.gene005324:CDS 35.0%
ATTTCACTTGATCGGAAGAT+TGG - chr1.1:62465573-62465592 MS.gene005324:CDS 35.0%
ATTTGTGTCAAAACAAGCTG+GGG - chr1.1:62465755-62465774 MS.gene005324:CDS 35.0%
CACAATCCATGAAATGTTTC+TGG + chr1.1:62466248-62466267 None:intergenic 35.0%
CTAAAAGGAACCATTCACTA+AGG - chr1.1:62465829-62465848 MS.gene005324:CDS 35.0%
CTGACTAAAAAGTCTATGGA+TGG - chr1.1:62467187-62467206 MS.gene005324:intron 35.0%
GAAACCTTTCTCAAAGCAAT+GGG - chr1.1:62466152-62466171 MS.gene005324:CDS 35.0%
GAAAGGGGAACATATTGAAT+TGG - chr1.1:62467021-62467040 MS.gene005324:CDS 35.0%
GAATTTGTGTCAAAACAAGC+TGG - chr1.1:62465753-62465772 MS.gene005324:CDS 35.0%
GACATTCCTCATATTCATCT+TGG + chr1.1:62466748-62466767 None:intergenic 35.0%
GATAGTAGTAGCGAATCATA+TGG - chr1.1:62466838-62466857 MS.gene005324:CDS 35.0%
GGAATTCCAAGATGAATATG+AGG - chr1.1:62466739-62466758 MS.gene005324:CDS 35.0%
GGTTGGGATGTAATCAAAAT+TGG - chr1.1:62466999-62467018 MS.gene005324:CDS 35.0%
TAATGTACCACACTTGGAAA+TGG - chr1.1:62465326-62465345 MS.gene005324:CDS 35.0%
TAGATGATTCACATGCCTTA+GGG + chr1.1:62466294-62466313 None:intergenic 35.0%
TAGTGCGAAGCAAATTTCTA+TGG + chr1.1:62465812-62465831 None:intergenic 35.0%
TCGGAAGATTGGAAAATACA+AGG - chr1.1:62465584-62465603 MS.gene005324:CDS 35.0%
TGAAACCTTTCTCAAAGCAA+TGG - chr1.1:62466151-62466170 MS.gene005324:CDS 35.0%
TGCATCTTGATCTGTTAGAA+TGG + chr1.1:62466195-62466214 None:intergenic 35.0%
TGGACATAAAAGCTCATTTC+AGG - chr1.1:62467041-62467060 MS.gene005324:CDS 35.0%
TGTTAGTAATGTACCACACT+TGG - chr1.1:62465320-62465339 MS.gene005324:CDS 35.0%
TTGTTTGTCTGTGGCTATTA+AGG + chr1.1:62466073-62466092 None:intergenic 35.0%
! CACTTTGAAAGAGTTGTCAA+CGG - chr1.1:62466596-62466615 MS.gene005324:CDS 35.0%
! GAAATTTGCTTCGCACTAAA+AGG - chr1.1:62465814-62465833 MS.gene005324:CDS 35.0%
! GGTTGAAGGTGTAGAAAAAT+TGG - chr1.1:62465263-62465282 MS.gene005324:CDS 35.0%
! TTTTCTACACCTTCAACCAT+TGG + chr1.1:62465261-62465280 None:intergenic 35.0%
!! ATTGTATTGGCTGATGAATC+TGG - chr1.1:62465708-62465727 MS.gene005324:CDS 35.0%
!! CAAGGTTATTGACTTTGTAG+CGG - chr1.1:62465602-62465621 MS.gene005324:CDS 35.0%
!!! AGAATTTTTTAGTGGATGGG+AGG - chr1.1:62466379-62466398 MS.gene005324:intron 35.0%
AAAGAGTTGTCAACGGAAAG+CGG - chr1.1:62466603-62466622 MS.gene005324:CDS 40.0%
AACAGATCAAGATGCAGCAA+TGG - chr1.1:62466199-62466218 MS.gene005324:CDS 40.0%
AACAGGATGTTTAGCAGGTA+TGG - chr1.1:62465548-62465567 MS.gene005324:CDS 40.0%
ACATCCAATCCAATGGTTGA+AGG - chr1.1:62465249-62465268 MS.gene005324:CDS 40.0%
ACTAGAACAGGATGTTTAGC+AGG - chr1.1:62465543-62465562 MS.gene005324:CDS 40.0%
AGGAACCATTCACTAAGGTA+TGG - chr1.1:62465834-62465853 MS.gene005324:CDS 40.0%
AGGCTAACAAATACTCCCAA+CGG + chr1.1:62466053-62466072 None:intergenic 40.0%
ATTCAAGGGAAAATGGGCAT+GGG - chr1.1:62466457-62466476 MS.gene005324:CDS 40.0%
CTTCACCATACCTTAGTGAA+TGG + chr1.1:62465842-62465861 None:intergenic 40.0%
GATTGTGCACTTGGCATATA+AGG - chr1.1:62466261-62466280 MS.gene005324:CDS 40.0%
GTCATGCCAGAAACATTTCA+TGG - chr1.1:62466239-62466258 MS.gene005324:CDS 40.0%
GTGAATGATATCGTGCAACA+GGG - chr1.1:62465285-62465304 MS.gene005324:CDS 40.0%
TACACCTTCAACCATTGGAT+TGG + chr1.1:62465256-62465275 None:intergenic 40.0%
TCAAACTCCATTTCCAAGTG+TGG + chr1.1:62465336-62465355 None:intergenic 40.0%
TCACTAAGGTATGGTGAAGT+AGG - chr1.1:62465843-62465862 MS.gene005324:CDS 40.0%
TCTATGGATGGAGAGTCAAT+TGG - chr1.1:62467199-62467218 MS.gene005324:intron 40.0%
TCTTGGAGTGTAAACATTGC+CGG + chr1.1:62466711-62466730 None:intergenic 40.0%
TTGCTCAATTTAACTGCTCG+AGG + chr1.1:62467088-62467107 None:intergenic 40.0%
TTTGTGTCAAAACAAGCTGG+GGG - chr1.1:62465756-62465775 MS.gene005324:CDS 40.0%
! ATGCATTGATCTACACGAAG+AGG - chr1.1:62466352-62466371 MS.gene005324:CDS 40.0%
! CATTTCATGGATTGTGCACT+TGG - chr1.1:62466252-62466271 MS.gene005324:CDS 40.0%
! TGTCGAAAGTTTGAGACACA+TGG - chr1.1:62466881-62466900 MS.gene005324:CDS 40.0%
AACGTTGGACAAGAGATGCA+AGG - chr1.1:62466978-62466997 MS.gene005324:CDS 45.0%
ACCTTTCTCAAAGCAATGGG+TGG - chr1.1:62466155-62466174 MS.gene005324:CDS 45.0%
ATCCCTATGCTTGTACAAGC+CGG - chr1.1:62466689-62466708 MS.gene005324:CDS 45.0%
CCTTTCTCAAAGCAATGGGT+GGG - chr1.1:62466156-62466175 MS.gene005324:CDS 45.0%
GATTCAAGGGAAAATGGGCA+TGG - chr1.1:62466456-62466475 MS.gene005324:CDS 45.0%
GCTGGGGGAATAAACAATGT+TGG - chr1.1:62465771-62465790 MS.gene005324:CDS 45.0%
GGAAATGGAGTTTGAGTCTG+AGG - chr1.1:62465341-62465360 MS.gene005324:CDS 45.0%
GGTGAATGATATCGTGCAAC+AGG - chr1.1:62465284-62465303 MS.gene005324:CDS 45.0%
TCAAGATGCAGCAATGGCTA+AGG - chr1.1:62466205-62466224 MS.gene005324:CDS 45.0%
TGGACAAGAGATGCAAGGTT+GGG - chr1.1:62466983-62467002 MS.gene005324:CDS 45.0%
TTGGACAAGAGATGCAAGGT+TGG - chr1.1:62466982-62467001 MS.gene005324:CDS 45.0%
AAAATCTTACCGGTGCCCAG+AGG + chr1.1:62465088-62465107 None:intergenic 50.0%
CATACTCAGCTGGCAGAAGA+AGG + chr1.1:62465638-62465657 None:intergenic 50.0%
GCTCGAGCAGAAACTAGAAC+AGG - chr1.1:62465531-62465550 MS.gene005324:CDS 50.0%
GTGCGAAAGACTTTCACTGC+AGG - chr1.1:62466485-62466504 MS.gene005324:CDS 50.0%
!! CCCACCCATTGCTTTGAGAA+AGG + chr1.1:62466159-62466178 None:intergenic 50.0%
!! TGCCGGCTTGTACAAGCATA+GGG + chr1.1:62466694-62466713 None:intergenic 50.0%
!! TTGCCGGCTTGTACAAGCAT+AGG + chr1.1:62466695-62466714 None:intergenic 50.0%
CACCGTTCATCACCACCTCT+GGG - chr1.1:62465070-62465089 MS.gene005324:CDS 55.0%
TCACCGTTCATCACCACCTC+TGG - chr1.1:62465069-62465088 MS.gene005324:CDS 55.0%
TGCTCCAAAGAGGGTAGACG+CGG - chr1.1:62465477-62465496 MS.gene005324:CDS 55.0%
! GGAGTTTGAGTCTGAGGCCT+TGG - chr1.1:62465347-62465366 MS.gene005324:CDS 55.0%
! TGCCCAGAGGTGGTGATGAA+CGG + chr1.1:62465075-62465094 None:intergenic 55.0%
ATCTTACCGGTGCCCAGAGG+TGG + chr1.1:62465085-62465104 None:intergenic 60.0%
GACACCGCGTCTACCCTCTT+TGG + chr1.1:62465484-62465503 None:intergenic 60.0%
TCATCACCACCTCTGGGCAC+CGG - chr1.1:62465076-62465095 MS.gene005324:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 62465031 62467267 62465031 ID=MS.gene005324
chr1.1 mRNA 62465031 62467267 62465031 ID=MS.gene005324.t1;Parent=MS.gene005324
chr1.1 exon 62467201 62467267 62467201 ID=MS.gene005324.t1.exon1;Parent=MS.gene005324.t1
chr1.1 CDS 62467201 62467267 62467201 ID=cds.MS.gene005324.t1;Parent=MS.gene005324.t1
chr1.1 exon 62466438 62467063 62466438 ID=MS.gene005324.t1.exon2;Parent=MS.gene005324.t1
chr1.1 CDS 62466438 62467063 62466438 ID=cds.MS.gene005324.t1;Parent=MS.gene005324.t1
chr1.1 exon 62465031 62466371 62465031 ID=MS.gene005324.t1.exon3;Parent=MS.gene005324.t1
chr1.1 CDS 62465031 62466371 62465031 ID=cds.MS.gene005324.t1;Parent=MS.gene005324.t1
Gene Sequence

>MS.gene005324

ATGGTAGAAACGACACACGTAGAACATGATGCAAATCTTCACCGTTCATCACCACCTCTGGGCACCGGTAAGATTTTGTTCTTTGTCGATGAATATACACACGTAGAACATGAATATTTGAAAGTATTTGGACAATATAAAATTAATATAAAATGTCTATGAATATTAATATGTTGACTTTATATTTTTTTTTCCATTTTCTAGATAATGTGAATATTACATCCAATCCAATGGTTGAAGGTGTAGAAAAATTGGTGAATGATATCGTGCAACAGGGAAAAGAGAGTACTGTTAGTAATGTACCACACTTGGAAATGGAGTTTGAGTCTGAGGCCTTGGCATATGAATTTTATAATGAGTATAGTAGGAAATGTGGTTTTGGTATCCGTCGAGAATATGCAAATAAAAGCAAAAAAGATGGAGTTCTTACTTCAAGAAAATTTGTGTGCTCCAAAGAGGGTAGACGCGGTGTCGACAAACGTGATTACTTAACAAAAGAGGCTCGAGCAGAAACTAGAACAGGATGTTTAGCAGGTATGGTTATTTCACTTGATCGGAAGATTGGAAAATACAAGGTTATTGACTTTGTAGCGGAACATAATCACCTTCTTCTGCCAGCTGAGTATGTTTATATGATTCGCTCTCATCGACATATATCTGAGTCACAAGCATCCCAAATTGTATTGGCTGATGAATCTGGATTGAAACCAAAAGATTTTCATGAATTTGTGTCAAAACAAGCTGGGGGAATAAACAATGTTGGTTACACAAGACAAGACCATAGAAATTTGCTTCGCACTAAAAGGAACCATTCACTAAGGTATGGTGAAGTAGGTGCATTACTAATACATTTCAAAAAGTAAAGTGAAAATCCTTCATTCTTTTATGAGTTTCAGATGGATGTTGAAGAGCAAATAACAAACATATTTTGGGCCGATGCACAAATGATAAATGATTATGGGTACTTTGGTGATGTTGTCACTTTTGACACCACATTCAAGACAAATAAGAATTATCGTCCGTTGGGAGTATTTGTTAGCCTTAATAGCCACAGACAAACAATTGTTTATGGGGCAACATTGTTATATGATGAAACAATCCCTTCTTTTCAATGGTTATTTGAAACCTTTCTCAAAGCAATGGGTGGGAAAAAGCCTAAAACCATTCTAACAGATCAAGATGCAGCAATGGCTAAGGCTCTTTCTATAGTCATGCCAGAAACATTTCATGGATTGTGCACTTGGCATATAAGGGAAAATGCCCTAAGGCATGTGAATCATCTATATCAACAGAGCTCACAATTCTGCTCAGATTTTGAAGCATGCATTGATCTACACGAAGAGGAAGAAGAATTTTTTAGTGGATGGGAGGCTTTACTAGTTGAGCATAATGTTTCTAAAGACTCATGGCTCCATAATATTTTTCGATTCAAGGGAAAATGGGCATGGGCTTATGTGCGAAAGACTTTCACTGCAGGTATGAGATCTACTCAATTAAGTGAGAGTTTTAATGCTGATTTAAAAAATCATTTGAAGTCAGATTTAAATTTGATACAATTTTTCACACACTTTGAAAGAGTTGTCAACGGAAAGCGGAATAATGAGTCAGAAGCTGAATATGAGTCGAGACAAAAGTTGCCAAGATTGAAAATGAAAAAGATCCCTATGCTTGTACAAGCCGGCAATGTTTACACTCCAAGAATTTTTGAGGAATTCCAAGATGAATATGAGGAATGTCAAGCTTCTTGTTTAAGAAATTTGAAAGAAGGGTCATATGTTGTTACAAATTATGATAACAGCAAGGAGCGGATAGTAGTAGCGAATCATATGGATAAAACAGTTGCTTGTGATTGTCGAAAGTTTGAGACACATGGAATCTTATGCAGTCATGCTTTGAAAGTTTTAGATTTTATGAATGTTAAGCTAATTCCTCAGCAATATATACTAAAACGTTGGACAAGAGATGCAAGGTTGGGATGTAATCAAAATTGGAAAGGGGAACATATTGAATTGGACATAAAAGCTCATTTCAGGAAACGATACAATGAGTTATGTCCTCGAGCAGTTAAATTGAGCAATAGAGCATCAGAATCTCATGAAACTTACACATTTTTGCCCAAAGTTTATGAAGAGTCTCATAAAATATTTGAAGATATGCTGACTAAAAAGTCTATGGATGGAGAGTCAATTGGAATGAGTCATGTGTCTATATCAATTGCAAATGAAAAAAAGGATTAG
Protein sequence

>MS.gene005324.t1

MVETTHVEHDANLHRSSPPLGTDNVNITSNPMVEGVEKLVNDIVQQGKESTVSNVPHLEMEFESEALAYEFYNEYSRKCGFGIRREYANKSKKDGVLTSRKFVCSKEGRRGVDKRDYLTKEARAETRTGCLAGMVISLDRKIGKYKVIDFVAEHNHLLLPAEYVYMIRSHRHISESQASQIVLADESGLKPKDFHEFVSKQAGGINNVGYTRQDHRNLLRTKRNHSLRYGEMDVEEQITNIFWADAQMINDYGYFGDVVTFDTTFKTNKNYRPLGVFVSLNSHRQTIVYGATLLYDETIPSFQWLFETFLKAMGGKKPKTILTDQDAAMAKALSIVMPETFHGLCTWHIRENALRHVNHLYQQSSQFCSDFEACIDLHEEEEEFFSGWEALLVEHNVSKDSWLHNIFRFKGKWAWAYVRKTFTAGMRSTQLSESFNADLKNHLKSDLNLIQFFTHFERVVNGKRNNESEAEYESRQKLPRLKMKKIPMLVQAGNVYTPRIFEEFQDEYEECQASCLRNLKEGSYVVTNYDNSKERIVVANHMDKTVACDCRKFETHGILCSHALKVLDFMNVKLIPQQYILKRWTRDARLGCNQNWKGEHIELDIKAHFRKRYNELCPRAVKLSNRASESHETYTFLPKVYEESHKIFEDMLTKKSMDGESIGMSHVSISIANEKKD