Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005493.t1 | AES61616.2 | 86.3 | 446 | 38 | 2 | 1 | 424 | 1 | 445 | 6.00E-206 | 726.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005493.t1 | Q8VZL6 | 59.4 | 414 | 137 | 9 | 2 | 397 | 5 | 405 | 3.2e-123 | 443.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005493.t1 | G7ICU5 | 86.3 | 446 | 38 | 2 | 1 | 424 | 1 | 445 | 4.3e-206 | 726.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene005493.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005493.t1 | MTR_1g086590 | 90.583 | 446 | 19 | 2 | 1 | 424 | 1 | 445 | 0.0 | 817 |
| MS.gene005493.t1 | MTR_1g086590 | 92.584 | 418 | 24 | 3 | 1 | 412 | 1 | 417 | 0.0 | 784 |
| MS.gene005493.t1 | MTR_1g086590 | 95.491 | 377 | 16 | 1 | 1 | 377 | 1 | 376 | 0.0 | 738 |
| MS.gene005493.t1 | MTR_1g086590 | 95.062 | 324 | 15 | 1 | 1 | 324 | 1 | 323 | 0.0 | 627 |
| MS.gene005493.t1 | MTR_7g109870 | 68.357 | 414 | 102 | 5 | 1 | 398 | 1 | 401 | 0.0 | 561 |
| MS.gene005493.t1 | MTR_7g109870 | 67.866 | 417 | 103 | 5 | 1 | 398 | 1 | 405 | 0.0 | 560 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005493.t1 | AT2G47210 | 59.277 | 415 | 138 | 9 | 2 | 398 | 5 | 406 | 5.11e-165 | 473 |
| MS.gene005493.t1 | AT2G47210 | 59.277 | 415 | 138 | 9 | 2 | 398 | 5 | 406 | 5.11e-165 | 473 |
| MS.gene005493.t1 | AT2G47210 | 59.277 | 415 | 138 | 9 | 2 | 398 | 5 | 406 | 5.11e-165 | 473 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCAGATTCAAATTGTAAA+TGG | 0.262132 | 1.1:-64287785 | MS.gene005493:CDS |
| GTTCCAGATAACACAAGTTC+TGG | 0.296830 | 1.1:-64283584 | MS.gene005493:CDS |
| TTATAGGTGTATGCACTTAC+TGG | 0.300360 | 1.1:-64290258 | MS.gene005493:intron |
| CGAGGAGTATGATAAATATT+TGG | 0.306985 | 1.1:-64287575 | MS.gene005493:CDS |
| TTTGTTTCAGCTGCAAAATA+AGG | 0.323047 | 1.1:-64283846 | MS.gene005493:intron |
| ATCAAACTTTGCTGGAACTT+GGG | 0.341194 | 1.1:-64285134 | MS.gene005493:CDS |
| ATCTCCTGTGAAACGTTATA+TGG | 0.347225 | 1.1:+64286355 | None:intergenic |
| CAGTTGATCTGTTTCCTCCT+TGG | 0.354649 | 1.1:+64286830 | None:intergenic |
| AAAGCCTCCTTCAGACAAAA+AGG | 0.356170 | 1.1:-64290187 | MS.gene005493:intron |
| ACGTGTTGATCAAACTTTGC+TGG | 0.359797 | 1.1:-64285142 | MS.gene005493:CDS |
| TTTCCCTCATTCAGGTCATT+TGG | 0.362778 | 1.1:-64288580 | MS.gene005493:intron |
| TGCAGTGGATCCACTTGTTC+AGG | 0.368298 | 1.1:-64286533 | MS.gene005493:intron |
| GATCAAACTTTGCTGGAACT+TGG | 0.378095 | 1.1:-64285135 | MS.gene005493:CDS |
| ATCGTGGAAGGCACGACTAT+TGG | 0.381129 | 1.1:+64285598 | None:intergenic |
| GTAAATGGTGTTCCACCAAC+AGG | 0.383590 | 1.1:-64287770 | MS.gene005493:CDS |
| TGCATTTACCTTGTTATACT+TGG | 0.387949 | 1.1:+64287733 | None:intergenic |
| GCTTTGTTTGAGAGAGGATC+AGG | 0.395914 | 1.1:+64286311 | None:intergenic |
| ATCCGTAGAAGATGGATTCC+TGG | 0.400770 | 1.1:+64286558 | None:intergenic |
| ATGGCGATGGAGGGCGGCGG+TGG | 0.400772 | 1.1:+64282112 | None:intergenic |
| TACAAATCGCAACACCATTC+CGG | 0.417236 | 1.1:+64283072 | None:intergenic |
| CTTGCTGAATCTTCAGAAAC+AGG | 0.417237 | 1.1:-64283976 | MS.gene005493:intron |
| ATGATCTTCAGCTTCATCAT+TGG | 0.419570 | 1.1:-64288523 | MS.gene005493:CDS |
| GTCCTTGCCATGCAGTATCC+CGG | 0.421755 | 1.1:-64286594 | MS.gene005493:intron |
| TCGAACTGTTAAAGAATTGA+AGG | 0.424156 | 1.1:-64286745 | MS.gene005493:CDS |
| AACCTATGCACTTGGACAAA+TGG | 0.426661 | 1.1:-64285205 | MS.gene005493:CDS |
| TGAGTTGGATGTTGCTTCAA+AGG | 0.427293 | 1.1:-64285689 | MS.gene005493:CDS |
| TCTTTAACAGTTCGAGATGA+AGG | 0.429916 | 1.1:+64286753 | None:intergenic |
| TTCCAGATAACACAAGTTCT+GGG | 0.430784 | 1.1:-64283583 | MS.gene005493:CDS |
| GATTTATTGTTATAGCTGAT+AGG | 0.439661 | 1.1:-64286779 | MS.gene005493:CDS |
| TGATCTTCAGCTTCATCATT+GGG | 0.440280 | 1.1:-64288522 | MS.gene005493:intron |
| TCCTGGCAATTAACATAGCC+CGG | 0.451028 | 1.1:+64286575 | None:intergenic |
| TATCAGCTATAACAATAAAT+CGG | 0.454968 | 1.1:+64286781 | None:intergenic |
| GCTTTCTGTTAGGTGGCAAC+CGG | 0.455766 | 1.1:-64283091 | MS.gene005493:intron |
| AAATCAACTGCTGGCATAAG+AGG | 0.458830 | 1.1:+64290225 | None:intergenic |
| GGGTCACACTGCGAAACAAC+GGG | 0.473685 | 1.1:-64283797 | MS.gene005493:CDS |
| TTTGCATCGAGGCGGAGATC+AGG | 0.475049 | 1.1:-64283618 | MS.gene005493:CDS |
| CTTGTTGCTTTGTTTGAGAG+AGG | 0.491846 | 1.1:+64286305 | None:intergenic |
| CGGCGAATGGCGATGGAGGG+CGG | 0.493726 | 1.1:+64282106 | None:intergenic |
| CTGTTAGGTGGCAACCGGAA+TGG | 0.501065 | 1.1:-64283086 | MS.gene005493:intron |
| CCCGGGCTATGTTAATTGCC+AGG | 0.503662 | 1.1:-64286576 | MS.gene005493:CDS |
| GGTGGAAGTTCTGTATTTGA+TGG | 0.504189 | 1.1:+64285619 | None:intergenic |
| TGATGGAGATACAGTTTCAC+CGG | 0.504548 | 1.1:+64285636 | None:intergenic |
| GATGGAGGGCGGCGGTGGAG+AGG | 0.505332 | 1.1:+64282117 | None:intergenic |
| TCAACATCGGCGAATGGCGA+TGG | 0.506601 | 1.1:+64282099 | None:intergenic |
| CAATCTGCAAGCTCATCAGC+TGG | 0.509587 | 1.1:-64285180 | MS.gene005493:CDS |
| CAACATCCAACTCAGACTCT+CGG | 0.518492 | 1.1:+64285698 | None:intergenic |
| AACATCCAACTCAGACTCTC+GGG | 0.521677 | 1.1:+64285699 | None:intergenic |
| TCAAACTTTGCTGGAACTTG+GGG | 0.524390 | 1.1:-64285133 | MS.gene005493:intron |
| AGATACAGTTTCACCGGGGT+TGG | 0.525293 | 1.1:+64285642 | None:intergenic |
| TTGAAGGATCGATATTATAG+TGG | 0.527157 | 1.1:-64286729 | MS.gene005493:intron |
| GTTGACCACACCTGAACAAG+TGG | 0.528927 | 1.1:+64286523 | None:intergenic |
| CTTTAACAGTTCGAGATGAA+GGG | 0.529292 | 1.1:+64286754 | None:intergenic |
| AACAGAGACAAATCAACTGC+TGG | 0.529934 | 1.1:+64290216 | None:intergenic |
| ACAAGAGCAAAGAGATGAAG+AGG | 0.530302 | 1.1:-64286287 | MS.gene005493:intron |
| ACATCGGCGAATGGCGATGG+AGG | 0.531173 | 1.1:+64282102 | None:intergenic |
| ATATAACGTTTCACAGGAGA+TGG | 0.536143 | 1.1:-64286353 | MS.gene005493:CDS |
| TGCCAGGAATCCATCTTCTA+CGG | 0.537155 | 1.1:-64286560 | MS.gene005493:CDS |
| TGCAGGTCAATTTGAAACCA+AGG | 0.539590 | 1.1:-64284055 | MS.gene005493:intron |
| CTAGAATTCTTCTCCTGAAG+TGG | 0.542194 | 1.1:+64290370 | None:intergenic |
| GTGGAAGGCACGACTATTGG+TGG | 0.544034 | 1.1:+64285601 | None:intergenic |
| ACTGCAACATCCGTAGAAGA+TGG | 0.546115 | 1.1:+64286550 | None:intergenic |
| CACCATTTGTCCAAGTGCAT+AGG | 0.547944 | 1.1:+64285203 | None:intergenic |
| TCCTTGCCATGCAGTATCCC+GGG | 0.551210 | 1.1:-64286593 | MS.gene005493:intron |
| TCATTCAGGTCATTTGGCGA+TGG | 0.553815 | 1.1:-64288574 | MS.gene005493:intron |
| CAGATTTCAACATCGGCGAA+TGG | 0.554680 | 1.1:+64282093 | None:intergenic |
| CGAATGGCGATGGAGGGCGG+CGG | 0.554829 | 1.1:+64282109 | None:intergenic |
| TACCATGCGAAGAGAGGCTA+TGG | 0.556004 | 1.1:+64285555 | None:intergenic |
| CAAGCTCATCAGCTGGACTT+CGG | 0.556264 | 1.1:-64285173 | MS.gene005493:CDS |
| AACTGTTTGACTTGTGTGAG+AGG | 0.559257 | 1.1:-64286812 | MS.gene005493:CDS |
| TATTCAAGAACCTATGCACT+TGG | 0.559755 | 1.1:-64285213 | MS.gene005493:CDS |
| TGCATTATCTGTCGATATCG+TGG | 0.571102 | 1.1:+64285582 | None:intergenic |
| TAGGTGTATGCACTTACTGG+TGG | 0.572395 | 1.1:-64290255 | MS.gene005493:CDS |
| AAAGAATAGTCCCCTGTTGG+TGG | 0.572554 | 1.1:+64287758 | None:intergenic |
| GCAGGTCAATTTGAAACCAA+GGG | 0.576834 | 1.1:-64284054 | MS.gene005493:intron |
| TTAACTTACCATGCGAAGAG+AGG | 0.578824 | 1.1:+64285549 | None:intergenic |
| GGAACCATATAACGTTTCAC+AGG | 0.582270 | 1.1:-64286359 | MS.gene005493:CDS |
| CTTTGTTTGAGAGAGGATCA+GGG | 0.585863 | 1.1:+64286312 | None:intergenic |
| GGTTGCCCGAGAGTCTGAGT+TGG | 0.587360 | 1.1:-64285704 | MS.gene005493:CDS |
| ATCTTCTACGGATGTTGCAG+TGG | 0.587379 | 1.1:-64286548 | MS.gene005493:CDS |
| ACTCGTGGCAGTTCTGATTG+TGG | 0.587493 | 1.1:-64282996 | MS.gene005493:intron |
| CGGGTCACACTGCGAAACAA+CGG | 0.588101 | 1.1:-64283798 | MS.gene005493:CDS |
| TACCATAGCCTCTCTTCGCA+TGG | 0.588204 | 1.1:-64285557 | MS.gene005493:intron |
| GTTTCAGCTGCAAAATAAGG+AGG | 0.600246 | 1.1:-64283843 | MS.gene005493:intron |
| ATTCTTCTCCTGAAGTGGGA+AGG | 0.601079 | 1.1:+64290375 | None:intergenic |
| CCTGGCAATTAACATAGCCC+GGG | 0.603428 | 1.1:+64286576 | None:intergenic |
| CATCGGCGAATGGCGATGGA+GGG | 0.606665 | 1.1:+64282103 | None:intergenic |
| CGAAACAACGGGAACACCAA+AGG | 0.608995 | 1.1:-64283786 | MS.gene005493:intron |
| TTGTGCAAACGTTACAGAGA+GGG | 0.610465 | 1.1:-64285665 | MS.gene005493:CDS |
| GCAAAAGAATAGTCCCCTGT+TGG | 0.610779 | 1.1:+64287755 | None:intergenic |
| TAAATGGTGTTCCACCAACA+GGG | 0.611976 | 1.1:-64287769 | MS.gene005493:CDS |
| GATGGAGATACAGTTTCACC+GGG | 0.612216 | 1.1:+64285637 | None:intergenic |
| CTGCAAAATAAGGAGGCTGA+AGG | 0.613211 | 1.1:-64283836 | MS.gene005493:CDS |
| TTATCTGTCGATATCGTGGA+AGG | 0.613435 | 1.1:+64285586 | None:intergenic |
| TAGAATTCTTCTCCTGAAGT+GGG | 0.615625 | 1.1:+64290371 | None:intergenic |
| TCCAGATAACACAAGTTCTG+GGG | 0.616535 | 1.1:-64283582 | MS.gene005493:CDS |
| CTGTTTCTGAAGATTCAGCA+AGG | 0.629529 | 1.1:+64283977 | None:intergenic |
| GAAGATGCAGATTTCAACAT+CGG | 0.639113 | 1.1:+64282086 | None:intergenic |
| CATAACAAGATACACGGACG+AGG | 0.639523 | 1.1:-64287593 | MS.gene005493:CDS |
| AGCAGCGAACTCAAAACTCG+TGG | 0.660025 | 1.1:-64283011 | MS.gene005493:CDS |
| ACGTTTCACAGGAGATGGAG+CGG | 0.669816 | 1.1:-64286348 | MS.gene005493:CDS |
| CCAGATAACACAAGTTCTGG+GGG | 0.671324 | 1.1:-64283581 | MS.gene005493:intron |
| TGCCGACAGCGTTTGCATCG+AGG | 0.672897 | 1.1:-64283629 | MS.gene005493:intron |
| TCAGGAGAAGAATTCTAGCG+AGG | 0.675024 | 1.1:-64290365 | MS.gene005493:intron |
| GTTACAGAGAGGGCCAACCC+CGG | 0.680256 | 1.1:-64285655 | MS.gene005493:CDS |
| ATTTATGTAGAAATGGACCA+AGG | 0.683713 | 1.1:-64286847 | MS.gene005493:intron |
| TGAATTACGCATAACGACCA+AGG | 0.689788 | 1.1:-64286132 | MS.gene005493:intron |
| AAATGGTGTTCCACCAACAG+GGG | 0.698079 | 1.1:-64287768 | MS.gene005493:CDS |
| TGTTGACATAACAAGATACA+CGG | 0.700844 | 1.1:-64287599 | MS.gene005493:CDS |
| CTTGTGCAAACGTTACAGAG+AGG | 0.710038 | 1.1:-64285666 | MS.gene005493:CDS |
| CGACAGCGTTTGCATCGAGG+CGG | 0.711723 | 1.1:-64283626 | MS.gene005493:intron |
| TATGTAGAAATGGACCAAGG+AGG | 0.727348 | 1.1:-64286844 | MS.gene005493:intron |
| ATGGAGATACAGTTTCACCG+GGG | 0.808137 | 1.1:+64285638 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 64282071 | 64290437 | 64282071 | ID=MS.gene005493 |
| chr1.1 | mRNA | 64282071 | 64290437 | 64282071 | ID=MS.gene005493.t1;Parent=MS.gene005493 |
| chr1.1 | exon | 64290366 | 64290437 | 64290366 | ID=MS.gene005493.t1.exon1;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64290366 | 64290437 | 64290366 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64290188 | 64290274 | 64290188 | ID=MS.gene005493.t1.exon2;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64290188 | 64290274 | 64290188 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64288523 | 64288588 | 64288523 | ID=MS.gene005493.t1.exon3;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64288523 | 64288588 | 64288523 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64287742 | 64287804 | 64287742 | ID=MS.gene005493.t1.exon4;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64287742 | 64287804 | 64287742 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64287567 | 64287623 | 64287567 | ID=MS.gene005493.t1.exon5;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64287567 | 64287623 | 64287567 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64286730 | 64286859 | 64286730 | ID=MS.gene005493.t1.exon6;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64286730 | 64286859 | 64286730 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64286534 | 64286601 | 64286534 | ID=MS.gene005493.t1.exon7;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64286534 | 64286601 | 64286534 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64286288 | 64286380 | 64286288 | ID=MS.gene005493.t1.exon8;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64286288 | 64286380 | 64286288 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64286133 | 64286180 | 64286133 | ID=MS.gene005493.t1.exon9;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64286133 | 64286180 | 64286133 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64285558 | 64285725 | 64285558 | ID=MS.gene005493.t1.exon10;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64285558 | 64285725 | 64285558 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64285134 | 64285244 | 64285134 | ID=MS.gene005493.t1.exon11;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64285134 | 64285244 | 64285134 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64283977 | 64284072 | 64283977 | ID=MS.gene005493.t1.exon12;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64283977 | 64284072 | 64283977 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64283787 | 64283858 | 64283787 | ID=MS.gene005493.t1.exon13;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64283787 | 64283858 | 64283787 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64283582 | 64283642 | 64283582 | ID=MS.gene005493.t1.exon14;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64283582 | 64283642 | 64283582 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64282997 | 64283101 | 64282997 | ID=MS.gene005493.t1.exon15;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64282997 | 64283101 | 64282997 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| chr1.1 | exon | 64282071 | 64282147 | 64282071 | ID=MS.gene005493.t1.exon16;Parent=MS.gene005493.t1 |
| chr1.1 | CDS | 64282071 | 64282147 | 64282071 | ID=cds.MS.gene005493.t1;Parent=MS.gene005493.t1 |
| Gene Sequence |
| Protein sequence |
