Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00559.t1 | ABO33479.1 | 99.6 | 239 | 1 | 0 | 5 | 243 | 50 | 288 | 8.80E-132 | 479.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00559.t1 | O04135 | 86.1 | 245 | 27 | 3 | 5 | 243 | 154 | 397 | 2.4e-114 | 412.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00559.t1 | X2D315 | 99.6 | 239 | 1 | 0 | 5 | 243 | 141 | 379 | 6.4e-132 | 479.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene00559.t1 | TF | HB-KNOX |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00559.t1 | MTR_1g017080 | 98.354 | 243 | 4 | 0 | 1 | 243 | 137 | 379 | 2.07e-180 | 500 |
| MS.gene00559.t1 | MTR_2g024390 | 58.261 | 230 | 82 | 3 | 5 | 230 | 139 | 358 | 3.05e-77 | 238 |
| MS.gene00559.t1 | MTR_5g033720 | 55.505 | 218 | 92 | 2 | 5 | 220 | 94 | 308 | 1.59e-73 | 226 |
| MS.gene00559.t1 | MTR_5g085860 | 55.157 | 223 | 86 | 3 | 1 | 219 | 64 | 276 | 5.65e-64 | 201 |
| MS.gene00559.t1 | MTR_1g084060 | 47.009 | 234 | 111 | 4 | 1 | 226 | 37 | 265 | 4.71e-61 | 193 |
| MS.gene00559.t1 | MTR_1g084060 | 47.009 | 234 | 111 | 4 | 1 | 226 | 37 | 265 | 1.20e-60 | 193 |
| MS.gene00559.t1 | MTR_2g461240 | 31.579 | 228 | 134 | 5 | 1 | 206 | 112 | 339 | 7.79e-29 | 112 |
| MS.gene00559.t1 | MTR_1g012960 | 31.739 | 230 | 133 | 5 | 1 | 206 | 191 | 420 | 5.79e-27 | 107 |
| MS.gene00559.t1 | MTR_5g011070 | 29.707 | 239 | 128 | 5 | 1 | 206 | 45 | 276 | 1.38e-26 | 104 |
| MS.gene00559.t1 | MTR_4g116545 | 31.140 | 228 | 135 | 5 | 1 | 206 | 88 | 315 | 9.86e-26 | 103 |
| MS.gene00559.t1 | MTR_4g116545 | 31.140 | 228 | 135 | 5 | 1 | 206 | 88 | 315 | 1.42e-25 | 102 |
| MS.gene00559.t1 | MTR_3g106400 | 30.769 | 234 | 132 | 7 | 1 | 206 | 124 | 355 | 4.06e-24 | 99.4 |
| MS.gene00559.t1 | MTR_3g106400 | 30.769 | 234 | 132 | 7 | 1 | 206 | 124 | 355 | 4.26e-24 | 99.4 |
| MS.gene00559.t1 | MTR_3g106400 | 30.172 | 232 | 136 | 6 | 1 | 206 | 124 | 355 | 7.90e-24 | 99.0 |
| MS.gene00559.t1 | MTR_7g065050 | 39.726 | 73 | 42 | 1 | 136 | 208 | 296 | 366 | 8.82e-12 | 64.7 |
| MS.gene00559.t1 | MTR_4g019450 | 43.243 | 74 | 39 | 2 | 135 | 208 | 351 | 421 | 1.23e-11 | 64.3 |
| MS.gene00559.t1 | MTR_4g051532 | 50.909 | 55 | 27 | 0 | 154 | 208 | 409 | 463 | 3.89e-11 | 62.8 |
| MS.gene00559.t1 | MTR_3g112290 | 49.091 | 55 | 28 | 0 | 154 | 208 | 355 | 409 | 4.11e-11 | 62.8 |
| MS.gene00559.t1 | MTR_1g023050 | 49.091 | 55 | 28 | 0 | 154 | 208 | 363 | 417 | 7.25e-11 | 62.0 |
| MS.gene00559.t1 | MTR_3g112300 | 37.000 | 100 | 55 | 4 | 109 | 208 | 315 | 406 | 8.68e-11 | 61.6 |
| MS.gene00559.t1 | MTR_3g112300 | 49.091 | 55 | 28 | 0 | 154 | 208 | 352 | 406 | 9.11e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00559.t1 | AT4G08150 | 81.967 | 244 | 38 | 5 | 5 | 243 | 156 | 398 | 6.94e-134 | 383 |
| MS.gene00559.t1 | AT1G62360 | 57.627 | 236 | 83 | 4 | 5 | 236 | 143 | 365 | 5.65e-75 | 232 |
| MS.gene00559.t1 | AT1G23380 | 55.556 | 216 | 89 | 3 | 5 | 218 | 107 | 317 | 6.31e-72 | 223 |
| MS.gene00559.t1 | AT1G23380 | 55.093 | 216 | 90 | 3 | 5 | 218 | 109 | 319 | 1.98e-71 | 221 |
| MS.gene00559.t1 | AT1G70510 | 52.336 | 214 | 99 | 1 | 5 | 218 | 91 | 301 | 1.81e-67 | 211 |
| MS.gene00559.t1 | AT1G70510 | 51.376 | 218 | 99 | 2 | 5 | 218 | 91 | 305 | 2.47e-65 | 205 |
| MS.gene00559.t1 | AT1G62990 | 30.252 | 238 | 125 | 7 | 1 | 206 | 47 | 275 | 1.69e-27 | 107 |
| MS.gene00559.t1 | AT5G25220 | 33.043 | 230 | 127 | 7 | 1 | 206 | 177 | 403 | 5.25e-27 | 107 |
| MS.gene00559.t1 | AT5G25220 | 33.043 | 230 | 127 | 7 | 1 | 206 | 177 | 403 | 6.54e-27 | 107 |
| MS.gene00559.t1 | AT4G32040 | 28.139 | 231 | 137 | 4 | 1 | 206 | 136 | 362 | 1.14e-25 | 103 |
| MS.gene00559.t1 | AT5G11060 | 31.304 | 230 | 131 | 7 | 1 | 206 | 141 | 367 | 1.17e-25 | 103 |
| MS.gene00559.t1 | AT4G32040 | 28.139 | 231 | 137 | 4 | 1 | 206 | 136 | 362 | 1.34e-25 | 103 |
| MS.gene00559.t1 | AT1G75430 | 49.091 | 55 | 28 | 0 | 154 | 208 | 209 | 263 | 1.10e-11 | 63.5 |
| MS.gene00559.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 154 | 208 | 358 | 412 | 1.40e-11 | 64.3 |
| MS.gene00559.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 154 | 208 | 358 | 412 | 1.40e-11 | 64.3 |
| MS.gene00559.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 154 | 208 | 358 | 412 | 1.40e-11 | 64.3 |
| MS.gene00559.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 154 | 208 | 358 | 412 | 1.40e-11 | 64.3 |
| MS.gene00559.t1 | AT1G19700 | 50.909 | 55 | 27 | 0 | 154 | 208 | 358 | 412 | 1.40e-11 | 64.3 |
| MS.gene00559.t1 | AT5G02030 | 47.273 | 55 | 29 | 0 | 154 | 208 | 355 | 409 | 5.57e-11 | 62.4 |
| MS.gene00559.t1 | AT2G27220 | 49.091 | 55 | 28 | 0 | 154 | 208 | 235 | 289 | 5.77e-11 | 62.4 |
| MS.gene00559.t1 | AT2G27220 | 49.091 | 55 | 28 | 0 | 154 | 208 | 235 | 289 | 5.77e-11 | 62.4 |
| MS.gene00559.t1 | AT2G27220 | 49.091 | 55 | 28 | 0 | 154 | 208 | 253 | 307 | 6.73e-11 | 62.0 |
| MS.gene00559.t1 | AT1G75410 | 49.091 | 55 | 28 | 0 | 154 | 208 | 353 | 407 | 6.98e-11 | 62.0 |
| MS.gene00559.t1 | AT1G75410 | 49.091 | 55 | 28 | 0 | 154 | 208 | 353 | 407 | 6.98e-11 | 62.0 |
Find 59 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAGAAGACATGCAGTTTA+TGG | 0.215872 | 1.4:-5613634 | MS.gene00559:CDS |
| GCTGATGGTCCCTACCGTTT+AGG | 0.271349 | 1.4:-5613555 | MS.gene00559:CDS |
| ACCAGTTATTTATTTGCTTC+TGG | 0.277102 | 1.4:+5613688 | None:intergenic |
| ACAGAGTGCAGCTCTTTATA+TGG | 0.322707 | 1.4:-5613592 | MS.gene00559:CDS |
| ACCATGAATTGGTCTAGTTC+TGG | 0.338115 | 1.4:+5622206 | None:intergenic |
| CTGATGGTCCCTACCGTTTA+GGG | 0.343258 | 1.4:-5613554 | MS.gene00559:CDS |
| CTAGACAAAAGCTACTTAAT+TGG | 0.349186 | 1.4:-5614950 | MS.gene00559:CDS |
| ACCAAACGAGCCACCACTTC+CGG | 0.349272 | 1.4:+5622296 | None:intergenic |
| ACCGGAAGTGGTGGCTCGTT+TGG | 0.365361 | 1.4:-5622297 | MS.gene00559:CDS |
| ACCAGAAGCAAATAAATAAC+TGG | 0.406580 | 1.4:-5613689 | MS.gene00559:CDS |
| ATACCCCTTGTACCATGAAT+TGG | 0.413591 | 1.4:+5622195 | None:intergenic |
| GAAAGGAAAATTACCTAAAG+AGG | 0.441916 | 1.4:-5614973 | MS.gene00559:CDS |
| AAATCCATAGCCTCTTGTAT+AGG | 0.443002 | 1.4:+5621944 | None:intergenic |
| GTAGCATTGGCTGAATCAAC+AGG | 0.445109 | 1.4:-5613717 | MS.gene00559:CDS |
| TTACAAATGGCCTTATCCTT+CGG | 0.445371 | 1.4:-5614916 | MS.gene00559:intron |
| ATCATAATCATGGCCCTAAA+CGG | 0.455352 | 1.4:+5613541 | None:intergenic |
| ATTGGCTGAATCAACAGGTT+TGG | 0.461797 | 1.4:-5613712 | MS.gene00559:CDS |
| TCCAGAACTAGACCAATTCA+TGG | 0.475218 | 1.4:-5622207 | MS.gene00559:intron |
| CACTTTGCAATGGACCCCTT+CGG | 0.484187 | 1.4:-5621892 | MS.gene00559:CDS |
| GGTGGGAATTGCATTACAAA+TGG | 0.484691 | 1.4:-5614929 | MS.gene00559:CDS |
| TCTGCTCGAGGATCAATCTC+TGG | 0.491728 | 1.4:+5615080 | None:intergenic |
| GATTGGAGCTCCACCGGAAG+TGG | 0.502488 | 1.4:-5622309 | MS.gene00559:CDS |
| GGACCCCTTCGGATCTTCCC+TGG | 0.503797 | 1.4:-5621881 | MS.gene00559:intron |
| TCAATCAAAGGAAGAGGCAC+TGG | 0.507111 | 1.4:-5613662 | MS.gene00559:CDS |
| TTATAACGTACCGAAGGATA+AGG | 0.509147 | 1.4:+5614906 | None:intergenic |
| CACTATATTTCTTCAGCAAG+TGG | 0.509181 | 1.4:+5615039 | None:intergenic |
| GACAAAAGCTACTTAATTGG+TGG | 0.509834 | 1.4:-5614947 | MS.gene00559:CDS |
| TTATATGGATGGTCATTACA+TGG | 0.510806 | 1.4:-5613577 | MS.gene00559:CDS |
| AAGGCCTATACAAGAGGCTA+TGG | 0.514686 | 1.4:-5621948 | MS.gene00559:CDS |
| TGGCTCGTTTGGTTGCGTCT+AGG | 0.518848 | 1.4:-5622286 | MS.gene00559:CDS |
| GAGGATCAAGAGAATAGTGG+TGG | 0.527205 | 1.4:-5615119 | MS.gene00559:CDS |
| AATTCACGGTCTTCTGCTCG+AGG | 0.528363 | 1.4:+5615068 | None:intergenic |
| CACTTGCTGAAGAAATATAG+TGG | 0.533238 | 1.4:-5615038 | MS.gene00559:CDS |
| GAAGAGGATCAAGAGAATAG+TGG | 0.545053 | 1.4:-5615122 | MS.gene00559:CDS |
| TATGTAGATTGGAGCTCCAC+CGG | 0.547316 | 1.4:-5622315 | MS.gene00559:intron |
| GGAATCAGAGAAGGTAGCAT+TGG | 0.547951 | 1.4:-5613730 | MS.gene00559:CDS |
| ATAAACTGCATGTCTTCTGA+TGG | 0.556878 | 1.4:+5613636 | None:intergenic |
| AGACATGCAGTTTATGGTGA+TGG | 0.558113 | 1.4:-5613628 | MS.gene00559:CDS |
| CAGCTAAATACACTTTGCAA+TGG | 0.562110 | 1.4:-5621902 | MS.gene00559:CDS |
| TAATCATGGCCCTAAACGGT+AGG | 0.564867 | 1.4:+5613545 | None:intergenic |
| TGGAGCTCCACCGGAAGTGG+TGG | 0.570424 | 1.4:-5622306 | MS.gene00559:CDS |
| GGTTCATCAATCAAAGGAAG+AGG | 0.570775 | 1.4:-5613668 | MS.gene00559:CDS |
| AGTGCAGCTCTTTATATGGA+TGG | 0.581356 | 1.4:-5613588 | MS.gene00559:CDS |
| ATAACTGGTTCATCAATCAA+AGG | 0.592016 | 1.4:-5613674 | MS.gene00559:CDS |
| ACAAAAGCTACTTAATTGGT+GGG | 0.596461 | 1.4:-5614946 | MS.gene00559:CDS |
| ACTCACCAGGGAAGATCCGA+AGG | 0.608503 | 1.4:+5621876 | None:intergenic |
| AGGCGTTGGTTCATCAGAAG+AGG | 0.623533 | 1.4:-5615138 | MS.gene00559:CDS |
| AATCATGGCCCTAAACGGTA+GGG | 0.623950 | 1.4:+5613546 | None:intergenic |
| ATGCAGTTTATGGTGATGGA+TGG | 0.624056 | 1.4:-5613624 | MS.gene00559:CDS |
| ATATAAAGAGCTGCACTCTG+TGG | 0.627158 | 1.4:+5613594 | None:intergenic |
| AAACGAGCCACCACTTCCGG+TGG | 0.627824 | 1.4:+5622299 | None:intergenic |
| ATTAACAAGGCCTATACAAG+AGG | 0.632422 | 1.4:-5621954 | MS.gene00559:CDS |
| GATGGTCATTACATGGCTGA+TGG | 0.643940 | 1.4:-5613570 | MS.gene00559:CDS |
| CACTATATAGGAATCAGAGA+AGG | 0.647456 | 1.4:-5613739 | MS.gene00559:intron |
| CATGCTTGTGAAGTATAGAG+AGG | 0.656762 | 1.4:-5621978 | MS.gene00559:CDS |
| CTCACCAGGGAAGATCCGAA+GGG | 0.678902 | 1.4:+5621877 | None:intergenic |
| CTAGGCAAGAGTTTGAAGCA+AGG | 0.689197 | 1.4:-5622268 | MS.gene00559:CDS |
| AGTATAGAGAGGTATTAACA+AGG | 0.723207 | 1.4:-5621967 | MS.gene00559:CDS |
| TCACCAGGGAAGATCCGAAG+GGG | 0.724320 | 1.4:+5621878 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 5613548 | 5632954 | 5613548 | ID=MS.gene00559 |
| chr1.4 | mRNA | 5613548 | 5632954 | 5613548 | ID=MS.gene00559.t1;Parent=MS.gene00559 |
| chr1.4 | exon | 5632940 | 5632954 | 5632940 | ID=MS.gene00559.t1.exon1;Parent=MS.gene00559.t1 |
| chr1.4 | CDS | 5632940 | 5632954 | 5632940 | ID=cds.MS.gene00559.t1;Parent=MS.gene00559.t1 |
| chr1.4 | exon | 5622208 | 5622330 | 5622208 | ID=MS.gene00559.t1.exon2;Parent=MS.gene00559.t1 |
| chr1.4 | CDS | 5622208 | 5622330 | 5622208 | ID=cds.MS.gene00559.t1;Parent=MS.gene00559.t1 |
| chr1.4 | exon | 5621882 | 5622014 | 5621882 | ID=MS.gene00559.t1.exon3;Parent=MS.gene00559.t1 |
| chr1.4 | CDS | 5621882 | 5622014 | 5621882 | ID=cds.MS.gene00559.t1;Parent=MS.gene00559.t1 |
| chr1.4 | exon | 5614917 | 5615173 | 5614917 | ID=MS.gene00559.t1.exon4;Parent=MS.gene00559.t1 |
| chr1.4 | CDS | 5614917 | 5615173 | 5614917 | ID=cds.MS.gene00559.t1;Parent=MS.gene00559.t1 |
| chr1.4 | exon | 5613548 | 5613751 | 5613548 | ID=MS.gene00559.t1.exon5;Parent=MS.gene00559.t1 |
| chr1.4 | CDS | 5613548 | 5613751 | 5613548 | ID=cds.MS.gene00559.t1;Parent=MS.gene00559.t1 |
| Gene Sequence |
| Protein sequence |
