Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00561.t1 | XP_013465930.1 | 93.5 | 168 | 10 | 1 | 20 | 187 | 86 | 252 | 8.40E-82 | 313.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00561.t1 | F4JSU0 | 70.6 | 34 | 10 | 0 | 20 | 53 | 86 | 119 | 8.2e-09 | 62.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00561.t1 | A0A072VEE8 | 93.5 | 168 | 10 | 1 | 20 | 187 | 86 | 252 | 6.1e-82 | 313.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene00561.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00561.t1 | MTR_1g017000 | 93.452 | 168 | 10 | 1 | 20 | 187 | 86 | 252 | 6.34e-111 | 317 |
| MS.gene00561.t1 | MTR_6g009430 | 50.725 | 69 | 30 | 3 | 20 | 85 | 85 | 152 | 1.71e-12 | 65.1 |
| MS.gene00561.t1 | MTR_3g103570 | 41.096 | 73 | 43 | 0 | 20 | 92 | 86 | 158 | 1.99e-12 | 64.7 |
| MS.gene00561.t1 | MTR_7g102110 | 56.452 | 62 | 20 | 2 | 20 | 74 | 86 | 147 | 2.80e-12 | 63.9 |
| MS.gene00561.t1 | MTR_4g057635 | 70.588 | 34 | 10 | 0 | 20 | 53 | 86 | 119 | 2.04e-11 | 61.6 |
| MS.gene00561.t1 | MTR_6g090405 | 62.500 | 40 | 15 | 0 | 15 | 54 | 81 | 120 | 2.06e-11 | 61.2 |
| MS.gene00561.t1 | MTR_1g017140 | 67.647 | 34 | 11 | 0 | 20 | 53 | 86 | 119 | 5.14e-11 | 60.8 |
| MS.gene00561.t1 | MTR_5g014990 | 67.647 | 34 | 11 | 0 | 20 | 53 | 86 | 119 | 5.26e-11 | 60.5 |
| MS.gene00561.t1 | MTR_4g091490 | 53.333 | 45 | 19 | 1 | 20 | 64 | 86 | 128 | 5.85e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00561.t1 | AT5G16770 | 38.136 | 118 | 60 | 6 | 20 | 134 | 85 | 192 | 1.34e-12 | 65.1 |
| MS.gene00561.t1 | AT5G16770 | 38.136 | 118 | 60 | 6 | 20 | 134 | 85 | 192 | 1.34e-12 | 65.1 |
| MS.gene00561.t1 | AT5G16770 | 38.136 | 118 | 60 | 6 | 20 | 134 | 85 | 192 | 2.20e-12 | 64.7 |
| MS.gene00561.t1 | AT5G16770 | 38.136 | 118 | 60 | 6 | 20 | 134 | 85 | 192 | 2.20e-12 | 64.7 |
| MS.gene00561.t1 | AT5G57620 | 53.846 | 52 | 21 | 1 | 20 | 71 | 86 | 134 | 1.65e-11 | 62.0 |
| MS.gene00561.t1 | AT4G37780 | 70.588 | 34 | 10 | 0 | 20 | 53 | 86 | 119 | 2.21e-11 | 61.6 |
| MS.gene00561.t1 | AT3G23250 | 45.000 | 80 | 41 | 2 | 15 | 92 | 23 | 101 | 3.65e-11 | 60.5 |
| MS.gene00561.t1 | AT5G23000 | 63.158 | 38 | 14 | 0 | 15 | 52 | 81 | 118 | 8.09e-11 | 60.1 |
| MS.gene00561.t1 | AT3G23250 | 45.000 | 80 | 41 | 2 | 15 | 92 | 80 | 158 | 9.21e-11 | 59.7 |
| MS.gene00561.t1 | AT5G65790 | 69.697 | 33 | 10 | 0 | 20 | 52 | 86 | 118 | 9.63e-11 | 60.1 |
Find 29 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGAATCATTGATGGATTT+TGG | 0.132955 | 1.4:+5705512 | MS.gene00561:CDS |
| ACTTTATTGTCTAGCATAAT+TGG | 0.238985 | 1.4:-5705362 | None:intergenic |
| AAAACTGAAGAAGAAGATTT+TGG | 0.243833 | 1.4:+5705196 | MS.gene00561:CDS |
| CAGCAATATTATTAATCCTT+TGG | 0.356887 | 1.4:+5705430 | MS.gene00561:CDS |
| GATGCTGTAGTATCAGAAAT+TGG | 0.363867 | 1.4:+5705398 | MS.gene00561:CDS |
| TTTCACCCTCTATTCTCAAA+TGG | 0.370485 | 1.4:+5704208 | MS.gene00561:CDS |
| TCAAGCTGGTGATGAATCTA+TGG | 0.374214 | 1.4:+5705493 | MS.gene00561:CDS |
| TGAGTTAGTGATGGAGTTGA+AGG | 0.446977 | 1.4:-5705266 | None:intergenic |
| GCCAGGTAGTTTAGATGCTA+TGG | 0.455877 | 1.4:-5705138 | None:intergenic |
| TGCTACCCATTTGAGAATAG+AGG | 0.456147 | 1.4:-5704214 | None:intergenic |
| TGAATCTATGGAATCATTGA+TGG | 0.458518 | 1.4:+5705505 | MS.gene00561:CDS |
| TTCACCCTCTATTCTCAAAT+GGG | 0.465064 | 1.4:+5704209 | MS.gene00561:CDS |
| ACATCATTGTCTGTTCTGCC+AGG | 0.476737 | 1.4:-5705155 | None:intergenic |
| TCTATTCTCAAATGGGTAGC+AGG | 0.500149 | 1.4:+5704216 | MS.gene00561:CDS |
| TCTTAACTGCTTTCCTGAGT+GGG | 0.511907 | 1.4:+5705568 | MS.gene00561:CDS |
| AGCATAATTGGAAGTGGTGA+AGG | 0.515734 | 1.4:-5705350 | None:intergenic |
| TTGTCTAGCATAATTGGAAG+TGG | 0.518920 | 1.4:-5705356 | None:intergenic |
| AGAATAGAGGGTGAAAATGA+TGG | 0.524462 | 1.4:-5704201 | None:intergenic |
| TTGGAACTTTGAGTTAGTGA+TGG | 0.526757 | 1.4:-5705275 | None:intergenic |
| GAGTTAGTGATGGAGTTGAA+GGG | 0.551144 | 1.4:-5705265 | None:intergenic |
| ATCTTAACTGCTTTCCTGAG+TGG | 0.564962 | 1.4:+5705567 | MS.gene00561:CDS |
| TGGAGGCTTCACTGAAGAAG+AGG | 0.582932 | 1.4:+5704175 | MS.gene00561:CDS |
| ATAATTGGAAGTGGTGAAGG+TGG | 0.597724 | 1.4:-5705347 | None:intergenic |
| GCTACCCATTTGAGAATAGA+GGG | 0.601575 | 1.4:-5704213 | None:intergenic |
| TCTTGAGAAATTGACACCAA+AGG | 0.604458 | 1.4:-5705446 | None:intergenic |
| TAACTAAAATCAACCCACTC+AGG | 0.606546 | 1.4:-5705581 | None:intergenic |
| GCCATAGCATCTAAACTACC+TGG | 0.606568 | 1.4:+5705137 | MS.gene00561:CDS |
| ATTGCAATTCAAGATCAAGC+TGG | 0.635582 | 1.4:+5705479 | MS.gene00561:CDS |
| TCATTGACATGATCATCATG+AGG | 0.679338 | 1.4:-5705545 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTAAAATCATGTTAT+TGG | - | chr1.4:5704920-5704939 | None:intergenic | 10.0% |
| !! | ACTAAAATTTAAATTTCAGT+TGG | + | chr1.4:5704597-5704616 | MS.gene00561:intron | 15.0% |
| !! | CGTTTATAATAATAACAATT+TGG | - | chr1.4:5704298-5704317 | None:intergenic | 15.0% |
| !! | CTAAAATTTAAATTTCAGTT+GGG | + | chr1.4:5704598-5704617 | MS.gene00561:intron | 15.0% |
| !! | TACAACTTAATTAAATAAGT+TGG | - | chr1.4:5704254-5704273 | None:intergenic | 15.0% |
| !! | TTAAAAAAAGTGAGAAATTA+TGG | + | chr1.4:5704931-5704950 | MS.gene00561:intron | 15.0% |
| !!! | ATATTCTATAACCATTTTTT+GGG | - | chr1.4:5704885-5704904 | None:intergenic | 15.0% |
| !!! | TATATTCTATAACCATTTTT+TGG | - | chr1.4:5704886-5704905 | None:intergenic | 15.0% |
| !!! | TGAAATTTAAATTTTAGTCT+CGG | - | chr1.4:5704595-5704614 | None:intergenic | 15.0% |
| !!! | AAATTGATTTCTGAATTTTG+AGG | - | chr1.4:5705302-5705321 | None:intergenic | 20.0% |
| !!! | AATTGATTTCTGAATTTTGA+GGG | - | chr1.4:5705301-5705320 | None:intergenic | 20.0% |
| !!! | ATTTTTTATAGTCAGTTGAA+TGG | + | chr1.4:5705023-5705042 | MS.gene00561:intron | 20.0% |
| ! | ACAATGATGTGAAAAACTAT+TGG | + | chr1.4:5705168-5705187 | MS.gene00561:CDS | 25.0% |
| ! | ACAGAAAAAATGTGATTAGA+AGG | + | chr1.4:5704780-5704799 | MS.gene00561:intron | 25.0% |
| ! | ACTTTATTGTCTAGCATAAT+TGG | - | chr1.4:5705365-5705384 | None:intergenic | 25.0% |
| ! | AGAAATTATAACAACCAACT+GGG | + | chr1.4:5704517-5704536 | MS.gene00561:intron | 25.0% |
| ! | ATTCCTAGTTTATAACATCA+TGG | - | chr1.4:5704571-5704590 | None:intergenic | 25.0% |
| ! | CAGCAATATTATTAATCCTT+TGG | + | chr1.4:5705430-5705449 | MS.gene00561:CDS | 25.0% |
| ! | TTCCTAGTTTATAACATCAT+GGG | - | chr1.4:5704570-5704589 | None:intergenic | 25.0% |
| !! | AAAACTGAAGAAGAAGATTT+TGG | + | chr1.4:5705196-5705215 | MS.gene00561:CDS | 25.0% |
| !! | GATTTTTAATGTGTACAAGT+TGG | + | chr1.4:5704461-5704480 | MS.gene00561:intron | 25.0% |
| !!! | ATTTTTTGGGTCTTAATCTT+GGG | - | chr1.4:5704872-5704891 | None:intergenic | 25.0% |
| CAAGATTAAGACCCAAAAAA+TGG | + | chr1.4:5704871-5704890 | MS.gene00561:intron | 30.0% | |
| GAAATTATAACAACCAACTG+GGG | + | chr1.4:5704518-5704537 | MS.gene00561:intron | 30.0% | |
| GAGAAATTATAACAACCAAC+TGG | + | chr1.4:5704516-5704535 | MS.gene00561:intron | 30.0% | |
| GTACTATGAAACTAACTTTG+AGG | - | chr1.4:5705050-5705069 | None:intergenic | 30.0% | |
| TAATTTCTCACTTTGACTGA+AGG | - | chr1.4:5704671-5704690 | None:intergenic | 30.0% | |
| TAGTTTCATAGTACTTCAAG+AGG | + | chr1.4:5705056-5705075 | MS.gene00561:intron | 30.0% | |
| TGAATCTATGGAATCATTGA+TGG | + | chr1.4:5705505-5705524 | MS.gene00561:CDS | 30.0% | |
| TGAGAACTATTCAAACTTTG+AGG | - | chr1.4:5705088-5705107 | None:intergenic | 30.0% | |
| TTCCCATGATGTTATAAACT+AGG | + | chr1.4:5704565-5704584 | MS.gene00561:intron | 30.0% | |
| ! | AATGTTGGTCTAAGTGTAAT+TGG | + | chr1.4:5704709-5704728 | MS.gene00561:intron | 30.0% |
| ! | AATTTGTGATTTTGTGCAGA+TGG | + | chr1.4:5705111-5705130 | MS.gene00561:intron | 30.0% |
| ! | ATGTCGTATTTAGAGTTTGA+TGG | + | chr1.4:5704625-5704644 | MS.gene00561:intron | 30.0% |
| !!! | AATGTTGATTTTGTGAAGCA+GGG | - | chr1.4:5705325-5705344 | None:intergenic | 30.0% |
| !!! | ATGGAATCATTGATGGATTT+TGG | + | chr1.4:5705512-5705531 | MS.gene00561:CDS | 30.0% |
| !!! | CATTTTTTGGGTCTTAATCT+TGG | - | chr1.4:5704873-5704892 | None:intergenic | 30.0% |
| !!! | GTATTGTCATTTTCAGTCAA+TGG | - | chr1.4:5705242-5705261 | None:intergenic | 30.0% |
| !!! | TTTTTTGGGTCTTAATCTTG+GGG | - | chr1.4:5704871-5704890 | None:intergenic | 30.0% |
| AGAATAGAGGGTGAAAATGA+TGG | - | chr1.4:5704204-5704223 | None:intergenic | 35.0% | |
| ATTGCAATTCAAGATCAAGC+TGG | + | chr1.4:5705479-5705498 | MS.gene00561:CDS | 35.0% | |
| CACTTAGACCAACATTATGT+CGG | - | chr1.4:5704705-5704724 | None:intergenic | 35.0% | |
| GATGCTGTAGTATCAGAAAT+TGG | + | chr1.4:5705398-5705417 | MS.gene00561:CDS | 35.0% | |
| GGAGAGAAACTACTAAATGT+TGG | + | chr1.4:5704801-5704820 | MS.gene00561:intron | 35.0% | |
| TAACTAAAATCAACCCACTC+AGG | - | chr1.4:5705584-5705603 | None:intergenic | 35.0% | |
| TCATTGACATGATCATCATG+AGG | - | chr1.4:5705548-5705567 | None:intergenic | 35.0% | |
| TCTTGAGAAATTGACACCAA+AGG | - | chr1.4:5705449-5705468 | None:intergenic | 35.0% | |
| TGGTCTAAGTGTAATTGGAT+TGG | + | chr1.4:5704714-5704733 | MS.gene00561:intron | 35.0% | |
| TTCACCCTCTATTCTCAAAT+GGG | + | chr1.4:5704209-5704228 | MS.gene00561:CDS | 35.0% | |
| TTGTCTAGCATAATTGGAAG+TGG | - | chr1.4:5705359-5705378 | None:intergenic | 35.0% | |
| TTTCACCCTCTATTCTCAAA+TGG | + | chr1.4:5704208-5704227 | MS.gene00561:CDS | 35.0% | |
| ! | TTGGAACTTTGAGTTAGTGA+TGG | - | chr1.4:5705278-5705297 | None:intergenic | 35.0% |
| !! | GATTTCTGAATTTTGAGGGT+TGG | - | chr1.4:5705297-5705316 | None:intergenic | 35.0% |
| !!! | GAATGTTGATTTTGTGAAGC+AGG | - | chr1.4:5705326-5705345 | None:intergenic | 35.0% |
| !!! | TCATTGATGGATTTTGGCTT+TGG | + | chr1.4:5705518-5705537 | MS.gene00561:CDS | 35.0% |
| AGCATAATTGGAAGTGGTGA+AGG | - | chr1.4:5705353-5705372 | None:intergenic | 40.0% | |
| ATAATTGGAAGTGGTGAAGG+TGG | - | chr1.4:5705350-5705369 | None:intergenic | 40.0% | |
| ATCTTAACTGCTTTCCTGAG+TGG | + | chr1.4:5705567-5705586 | MS.gene00561:CDS | 40.0% | |
| GCTACCCATTTGAGAATAGA+GGG | - | chr1.4:5704216-5704235 | None:intergenic | 40.0% | |
| GGAAAGACCATAATCATCAC+AGG | - | chr1.4:5704850-5704869 | None:intergenic | 40.0% | |
| TCTATTCTCAAATGGGTAGC+AGG | + | chr1.4:5704216-5704235 | MS.gene00561:CDS | 40.0% | |
| TCTTAACTGCTTTCCTGAGT+GGG | + | chr1.4:5705568-5705587 | MS.gene00561:CDS | 40.0% | |
| TGCTACCCATTTGAGAATAG+AGG | - | chr1.4:5704217-5704236 | None:intergenic | 40.0% | |
| TGTCGAACCTGTGATGATTA+TGG | + | chr1.4:5704840-5704859 | MS.gene00561:intron | 40.0% | |
| ! | GAGTTAGTGATGGAGTTGAA+GGG | - | chr1.4:5705268-5705287 | None:intergenic | 40.0% |
| ! | TCAAGCTGGTGATGAATCTA+TGG | + | chr1.4:5705493-5705512 | MS.gene00561:CDS | 40.0% |
| ! | TGAGTTAGTGATGGAGTTGA+AGG | - | chr1.4:5705269-5705288 | None:intergenic | 40.0% |
| !! | CTGAAGAAGAAGATTTTGGC+AGG | + | chr1.4:5705200-5705219 | MS.gene00561:CDS | 40.0% |
| ACATCATTGTCTGTTCTGCC+AGG | - | chr1.4:5705158-5705177 | None:intergenic | 45.0% | |
| CCAACATTATGTCGGACTGT+CGG | - | chr1.4:5704697-5704716 | None:intergenic | 45.0% | |
| GCCAGGTAGTTTAGATGCTA+TGG | - | chr1.4:5705141-5705160 | None:intergenic | 45.0% | |
| GCCATAGCATCTAAACTACC+TGG | + | chr1.4:5705137-5705156 | MS.gene00561:CDS | 45.0% | |
| TGGGGTTGAGTCAACTTGAA+GGG | + | chr1.4:5704536-5704555 | MS.gene00561:intron | 45.0% | |
| CCGACAGTCCGACATAATGT+TGG | + | chr1.4:5704694-5704713 | MS.gene00561:intron | 50.0% | |
| CTGGGGTTGAGTCAACTTGA+AGG | + | chr1.4:5704535-5704554 | MS.gene00561:intron | 50.0% | |
| GGGGTTGAGTCAACTTGAAG+GGG | + | chr1.4:5704537-5704556 | MS.gene00561:intron | 50.0% | |
| TGGAGGCTTCACTGAAGAAG+AGG | + | chr1.4:5704175-5704194 | MS.gene00561:CDS | 50.0% | |
| ! | CAAGTTGACTCAACCCCAGT+TGG | - | chr1.4:5704534-5704553 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 5704174 | 5705625 | 5704174 | ID=MS.gene00561 |
| chr1.4 | mRNA | 5704174 | 5705625 | 5704174 | ID=MS.gene00561.t1;Parent=MS.gene00561 |
| chr1.4 | exon | 5704174 | 5704241 | 5704174 | ID=MS.gene00561.t1.exon1;Parent=MS.gene00561.t1 |
| chr1.4 | CDS | 5704174 | 5704241 | 5704174 | ID=cds.MS.gene00561.t1;Parent=MS.gene00561.t1 |
| chr1.4 | exon | 5705130 | 5705625 | 5705130 | ID=MS.gene00561.t1.exon2;Parent=MS.gene00561.t1 |
| chr1.4 | CDS | 5705130 | 5705625 | 5705130 | ID=cds.MS.gene00561.t1;Parent=MS.gene00561.t1 |
| Gene Sequence |
| Protein sequence |
