AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005623

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005623.t1 MTR_1g088860 88.660 194 13 2 1 185 1 194 3.97e-118 333
MS.gene005623.t1 MTR_4g119500 78.095 105 23 0 24 128 12 116 2.55e-61 186
MS.gene005623.t1 MTR_0392s0020 68.992 129 37 1 24 152 8 133 1.24e-60 185
MS.gene005623.t1 MTR_1g083070 74.000 100 26 0 34 133 4 103 5.87e-53 165
MS.gene005623.t1 MTR_4g133938 64.407 118 39 1 35 152 20 134 1.21e-52 165
MS.gene005623.t1 MTR_2g026710 64.407 118 36 1 33 150 16 127 8.52e-52 164
MS.gene005623.t1 MTR_8g093920 72.277 101 28 0 33 133 39 139 1.73e-51 164
MS.gene005623.t1 MTR_7g061270 62.162 111 42 0 32 142 24 134 9.57e-51 161
MS.gene005623.t1 MTR_7g100650 62.162 111 42 0 32 142 24 134 1.54e-50 160
MS.gene005623.t1 MTR_3g058980 60.504 119 47 0 33 151 26 144 1.74e-50 161
MS.gene005623.t1 MTR_5g095740 71.875 96 27 0 33 128 22 117 1.21e-49 159
MS.gene005623.t1 MTR_8g091720 71.429 98 28 0 33 130 27 124 2.80e-49 158
MS.gene005623.t1 MTR_1g072790 60.714 112 44 0 33 144 28 139 7.51e-49 156
MS.gene005623.t1 MTR_4g133952 63.462 104 37 1 26 128 49 152 5.31e-48 156
MS.gene005623.t1 MTR_1g039040 56.604 106 46 0 32 137 4 109 1.55e-42 141
MS.gene005623.t1 MTR_1g029070 44.954 109 60 0 19 127 37 145 2.92e-28 103
MS.gene005623.t1 MTR_5g095900 44.565 92 51 0 31 122 13 104 3.34e-21 86.3
MS.gene005623.t1 MTR_1g029100 40.000 80 48 0 39 118 7 86 2.03e-18 76.3
MS.gene005623.t1 MTR_2g056000 32.727 110 72 1 3 112 12 119 1.49e-17 76.6
MS.gene005623.t1 MTR_1g028480 46.875 64 34 0 64 127 19 82 4.14e-16 70.9
MS.gene005623.t1 MTR_4g112380 32.051 78 53 0 35 112 12 89 3.72e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005623.t1 AT2G47810 77.451 102 23 0 31 132 48 149 4.41e-58 179
MS.gene005623.t1 AT1G09030 57.143 140 56 1 32 171 1 136 8.78e-53 165
MS.gene005623.t1 AT5G47640 68.468 111 32 1 33 140 26 136 1.94e-52 166
MS.gene005623.t1 AT4G14540 75.000 96 24 0 33 128 20 115 5.39e-52 164
MS.gene005623.t1 AT2G13570 73.267 101 27 0 33 133 35 135 2.65e-51 164
MS.gene005623.t1 AT2G38880 64.865 111 38 1 32 141 19 129 2.89e-51 161
MS.gene005623.t1 AT2G38880 64.865 111 38 1 32 141 19 129 3.55e-51 161
MS.gene005623.t1 AT2G38880 64.865 111 38 1 32 141 19 129 3.55e-51 161
MS.gene005623.t1 AT2G38880 64.865 111 38 1 32 141 19 129 3.55e-51 161
MS.gene005623.t1 AT3G53340 50.336 149 67 1 32 180 27 168 5.18e-51 162
MS.gene005623.t1 AT3G53340 50.336 149 67 1 32 180 27 168 5.18e-51 162
MS.gene005623.t1 AT2G38880 65.455 110 37 1 32 141 19 127 3.30e-50 159
MS.gene005623.t1 AT2G37060 63.063 111 41 0 32 142 28 138 4.66e-50 159
MS.gene005623.t1 AT2G37060 63.063 111 41 0 32 142 28 138 4.66e-50 159
MS.gene005623.t1 AT2G37060 63.063 111 41 0 32 142 28 138 4.66e-50 159
MS.gene005623.t1 AT2G38880 73.118 93 25 0 32 124 19 111 2.15e-49 155
MS.gene005623.t1 AT3G53340 72.043 93 26 0 32 124 27 119 6.02e-49 155
MS.gene005623.t1 AT2G38880 73.118 93 25 0 32 124 19 111 6.21e-49 156
MS.gene005623.t1 AT2G38880 73.118 93 25 0 32 124 19 111 6.21e-49 156
MS.gene005623.t1 AT3G53340 69.697 99 29 1 32 130 27 124 7.90e-49 155
MS.gene005623.t1 AT3G53340 69.697 99 29 1 32 130 27 124 7.90e-49 155
MS.gene005623.t1 AT2G38880 73.118 93 25 0 32 124 19 111 9.08e-49 155
MS.gene005623.t1 AT2G38880 73.118 93 25 0 32 124 19 111 9.08e-49 155
MS.gene005623.t1 AT2G38880 73.118 93 25 0 32 124 19 111 9.08e-49 155
MS.gene005623.t1 AT5G47670 63.265 98 36 0 32 129 27 124 5.45e-46 150
MS.gene005623.t1 AT5G47670 62.626 99 37 0 31 129 55 153 1.27e-45 150
MS.gene005623.t1 AT5G47670 62.626 99 37 0 31 129 55 153 1.27e-45 150
MS.gene005623.t1 AT1G21970 50.862 116 57 0 19 134 44 159 1.37e-41 140
MS.gene005623.t1 AT5G23090 35.052 97 61 1 35 129 12 108 1.57e-17 75.9
MS.gene005623.t1 AT5G23090 38.462 78 48 0 35 112 12 89 1.26e-16 73.6
MS.gene005623.t1 AT5G23090 38.462 78 48 0 35 112 12 89 1.26e-16 73.6
MS.gene005623.t1 AT5G23090 38.462 78 48 0 35 112 12 89 1.26e-16 73.6
MS.gene005623.t1 AT5G08190 32.292 96 63 1 35 128 12 107 4.85e-16 72.0
MS.gene005623.t1 AT5G08190 34.615 78 51 0 35 112 12 89 6.92e-15 68.9

Find 0 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGGTATTTGTATAAGTAT+AGG - 8682:93845-93864 MS.gene005623:CDS 20.0%
!!! AAGGTATTTGTATAAGTATA+GGG - 8682:93846-93865 MS.gene005623:CDS 20.0%
! GAAACATATGATTATGAAGA+TGG - 8682:93949-93968 MS.gene005623:CDS 25.0%
! TTTGTATAAGTATAGGGAAT+TGG - 8682:93852-93871 MS.gene005623:CDS 25.0%
!! TTATACAAATACCTTTTGAG+TGG + 8682:93841-93860 None:intergenic 25.0%
CCTATTATTGAACATAGAAG+AGG + 8682:94010-94029 None:intergenic 30.0%
CCTCTTCTATGTTCAATAAT+AGG - 8682:94007-94026 MS.gene005623:CDS 30.0%
!! TCTTTGGAGATTTTTGCATT+TGG + 8682:93664-93683 None:intergenic 30.0%
!! TGATGTTGTTACCCTTATTT+TGG + 8682:93894-93913 None:intergenic 30.0%
!!! GAGTTTGTTAGTTTTGTAAC+TGG - 8682:93715-93734 MS.gene005623:CDS 30.0%
CATGGTTTCTTTAGCATCTT+TGG + 8682:93680-93699 None:intergenic 35.0%
GAAAGAGGGAACCAAAATAA+GGG - 8682:93880-93899 MS.gene005623:CDS 35.0%
TAAGTATAGGGAATTGGATG+CGG - 8682:93858-93877 MS.gene005623:CDS 35.0%
TTCCAATAGCTAATGTTGGT+CGG - 8682:93617-93636 MS.gene005623:CDS 35.0%
TTGCTTCCAATAGCTAATGT+TGG - 8682:93613-93632 MS.gene005623:CDS 35.0%
! GAATGGTGATGATGTTTGTT+GGG - 8682:93780-93799 MS.gene005623:CDS 35.0%
! TGAATGGTGATGATGTTTGT+TGG - 8682:93779-93798 MS.gene005623:CDS 35.0%
! TTGGAGATTTTTGCATTTGG+TGG + 8682:93661-93680 None:intergenic 35.0%
ATCCGACCAACATTAGCTAT+TGG + 8682:93622-93641 None:intergenic 40.0%
ATGGTAAGTCACAAACTCCA+AGG - 8682:93968-93987 MS.gene005623:CDS 40.0%
GAAGCAACATTCACTTTCCT+TGG + 8682:93988-94007 None:intergenic 40.0%
GGAAAGAGGGAACCAAAATA+AGG - 8682:93879-93898 MS.gene005623:CDS 40.0%
! ATGATGTTTGTTGGGCTTTG+GGG - 8682:93788-93807 MS.gene005623:CDS 40.0%
! GATGATGTTTGTTGGGCTTT+GGG - 8682:93787-93806 MS.gene005623:CDS 40.0%
! TGATGATGTTTGTTGGGCTT+TGG - 8682:93786-93805 MS.gene005623:CDS 40.0%
AACAGTGACCTTCAGTCAAG+GGG - 8682:94030-94049 MS.gene005623:CDS 45.0%
AGCATCTGACAAGTGTCACA+AGG - 8682:93741-93760 MS.gene005623:CDS 45.0%
ATTACGCTGAGCCACTCAAA+AGG - 8682:93827-93846 MS.gene005623:CDS 45.0%
GAACAGTGACCTTCAGTCAA+GGG - 8682:94029-94048 MS.gene005623:CDS 45.0%
AGGGAATTGGATGCGGAAAG+AGG - 8682:93865-93884 MS.gene005623:CDS 50.0%
GGAACAGTGACCTTCAGTCA+AGG - 8682:94028-94047 MS.gene005623:CDS 50.0%
GGGAATTGGATGCGGAAAGA+GGG - 8682:93866-93885 MS.gene005623:CDS 50.0%
! GAGAAGCGCAAGACAGTGAA+TGG - 8682:93763-93782 MS.gene005623:CDS 50.0%
!! CTCTGAGACACACTCTTGCA+TGG + 8682:93698-93717 None:intergenic 50.0%
!! GTTGGGCTTTGGGGACACTA+GGG - 8682:93797-93816 MS.gene005623:CDS 55.0%
!! TGTTGGGCTTTGGGGACACT+AGG - 8682:93796-93815 MS.gene005623:CDS 55.0%


Chromosome Type Strat End Strand Name
8682 gene 93502 94059 93502 ID=MS.gene005623
8682 mRNA 93502 94059 93502 ID=MS.gene005623.t1;Parent=MS.gene005623
8682 exon 93502 94059 93502 ID=MS.gene005623.t1.exon1;Parent=MS.gene005623.t1
8682 CDS 93502 94059 93502 ID=cds.MS.gene005623.t1;Parent=MS.gene005623.t1
Gene Sequence

>MS.gene005623

ATGGGTGATAACAACAAAGGGAAACTAGTCTCAGATAGAGAAGTCTTTAAGTACAACACAAATCTCACATCTCATGAGCAAGATCATGAAGTAATCAAAGAGCAAGATCGTTTGCTTCCAATAGCTAATGTTGGTCGGATCATGAAACAAATCTTGCCACCAAATGCAAAAATCTCCAAAGATGCTAAAGAAACCATGCAAGAGTGTGTCTCAGAGTTTGTTAGTTTTGTAACTGGTGAAGCATCTGACAAGTGTCACAAGGAGAAGCGCAAGACAGTGAATGGTGATGATGTTTGTTGGGCTTTGGGGACACTAGGGTTTGATGATTACGCTGAGCCACTCAAAAGGTATTTGTATAAGTATAGGGAATTGGATGCGGAAAGAGGGAACCAAAATAAGGGTAACAACATCAACAACAACAGTGATGAAATTGAAAACAATATTAATGAAACATATGATTATGAAGATGGTAAGTCACAAACTCCAAGGAAAGTGAATGTTGCTTCCTCTTCTATGTTCAATAATAGGAACAGTGACCTTCAGTCAAGGGGATTTTGA
Protein sequence

>MS.gene005623.t1

MGDNNKGKLVSDREVFKYNTNLTSHEQDHEVIKEQDRLLPIANVGRIMKQILPPNAKISKDAKETMQECVSEFVSFVTGEASDKCHKEKRKTVNGDDVCWALGTLGFDDYAEPLKRYLYKYRELDAERGNQNKGNNINNNSDEIENNINETYDYEDGKSQTPRKVNVASSSMFNNRNSDLQSRGF