AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005758

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005758.t1 MTR_1g090697 92.308 156 12 0 1 156 1 156 1.46e-103 293
MS.gene005758.t1 MTR_1g090710 72.611 157 42 1 1 156 1 157 2.62e-80 234
MS.gene005758.t1 MTR_5g047580 60.000 155 62 0 1 155 1 155 4.35e-63 191
MS.gene005758.t1 MTR_1g090783 61.688 154 59 0 3 156 2 155 6.39e-63 190
MS.gene005758.t1 MTR_1g084950 63.871 155 56 0 1 155 1 155 1.52e-60 184
MS.gene005758.t1 MTR_1g077390 58.065 155 65 0 1 155 1 155 1.84e-59 182
MS.gene005758.t1 MTR_1g077320 61.290 155 60 0 1 155 1 155 7.78e-59 180
MS.gene005758.t1 MTR_4g028720 60.000 155 62 0 1 155 1 155 2.20e-56 174
MS.gene005758.t1 MTR_7g011950 60.000 155 62 0 1 155 1 155 2.86e-56 174
MS.gene005758.t1 MTR_1g077300 56.129 155 66 1 1 155 1 153 2.96e-47 150
MS.gene005758.t1 MTR_5g047560 50.968 155 60 1 1 155 1 139 2.64e-46 147
MS.gene005758.t1 MTR_3g466830 43.871 155 86 1 3 156 2 156 2.36e-39 130
MS.gene005758.t1 MTR_3g466890 41.935 155 89 1 3 156 2 156 4.43e-37 125
MS.gene005758.t1 MTR_4g032620 36.709 158 98 2 1 156 1 158 3.72e-35 122
MS.gene005758.t1 MTR_3g065100 41.667 156 90 1 1 155 1 156 5.56e-34 119
MS.gene005758.t1 MTR_3g466980 35.714 154 98 1 1 153 1 154 6.89e-34 119
MS.gene005758.t1 MTR_4g031910 42.405 158 89 2 1 156 1 158 8.42e-33 116
MS.gene005758.t1 MTR_2g016210 41.667 156 89 2 1 154 1 156 1.02e-31 114
MS.gene005758.t1 MTR_8g036130 36.250 160 98 3 1 156 1 160 2.90e-31 112
MS.gene005758.t1 MTR_5g075380 35.669 157 100 1 1 156 1 157 3.95e-30 107
MS.gene005758.t1 MTR_4g063790 34.615 156 96 3 1 152 1 154 2.96e-28 103
MS.gene005758.t1 MTR_3g031240 34.177 158 102 2 1 156 1 158 8.49e-28 102
MS.gene005758.t1 MTR_2g035610 42.975 121 68 1 1 120 1 121 4.31e-27 98.6
MS.gene005758.t1 MTR_3g466900 40.476 126 74 1 32 156 2 127 6.14e-27 98.6
MS.gene005758.t1 MTR_4g032260 40.385 156 91 2 1 154 1 156 8.84e-27 99.0
MS.gene005758.t1 MTR_3g031100 33.121 157 103 2 1 155 1 157 9.81e-25 95.9
MS.gene005758.t1 MTR_3g466930 38.095 126 77 1 32 156 2 127 9.97e-25 92.8
MS.gene005758.t1 MTR_4g032290 39.873 158 93 2 1 156 1 158 1.78e-24 94.7
MS.gene005758.t1 MTR_4g028800 51.389 72 33 1 1 72 1 70 6.86e-23 86.3
MS.gene005758.t1 MTR_3g467080 29.936 157 108 2 1 155 1 157 4.63e-21 84.3
MS.gene005758.t1 MTR_4g019670 30.061 163 96 4 1 145 1 163 5.85e-18 80.1
MS.gene005758.t1 MTR_2g035580 29.032 155 109 1 1 154 1 155 6.99e-18 75.9
MS.gene005758.t1 MTR_7g106510 31.098 164 93 6 1 145 1 163 1.29e-17 79.0
MS.gene005758.t1 MTR_1g033930 47.872 94 48 1 64 156 22 115 1.32e-17 74.3
MS.gene005758.t1 MTR_3g093900 29.677 155 108 1 3 156 6 160 9.26e-17 76.6
MS.gene005758.t1 MTR_1g033370 39.796 98 58 1 60 156 24 121 7.13e-16 69.7
MS.gene005758.t1 MTR_3g465410 29.688 128 84 3 29 152 1 126 1.79e-14 66.6
MS.gene005758.t1 MTR_1g033790 44.681 94 51 1 64 156 22 115 2.63e-14 65.5
MS.gene005758.t1 MTR_7g055800 29.661 118 56 2 1 118 1 91 7.27e-14 63.5
MS.gene005758.t1 MTR_6g005450 30.469 128 76 4 1 115 1 128 7.01e-12 62.4
MS.gene005758.t1 MTR_6g018920 24.224 161 109 4 1 155 1 154 1.44e-11 61.2
MS.gene005758.t1 MTR_7g075850 31.373 153 86 4 1 148 1 139 3.37e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005758.t1 AT3G05860 41.611 149 86 1 1 148 1 149 3.05e-32 114
MS.gene005758.t1 AT1G65300 37.013 154 96 1 3 155 2 155 7.95e-32 115
MS.gene005758.t1 AT3G05860 41.611 149 86 1 1 148 1 149 1.69e-31 113
MS.gene005758.t1 AT3G05860 41.611 149 86 1 1 148 1 149 2.18e-31 113
MS.gene005758.t1 AT5G48670 42.949 156 88 1 1 155 1 156 4.44e-30 111
MS.gene005758.t1 AT1G65330 37.013 154 96 1 3 155 2 155 1.97e-29 108
MS.gene005758.t1 AT5G26630 38.462 156 95 1 1 155 1 156 9.34e-29 105
MS.gene005758.t1 AT1G22590 37.975 158 92 3 1 156 1 154 3.22e-27 100
MS.gene005758.t1 AT1G31640 36.667 150 93 2 3 151 2 150 5.41e-25 99.8
MS.gene005758.t1 AT5G26650 35.294 153 98 1 4 155 2 154 7.71e-25 98.2
MS.gene005758.t1 AT1G31630 31.788 151 101 2 3 152 2 151 2.78e-24 96.3
MS.gene005758.t1 AT5G27960 33.987 153 100 1 4 155 2 154 3.34e-23 93.2
MS.gene005758.t1 AT2G28700 33.858 127 77 2 1 123 1 124 1.66e-20 86.3
MS.gene005758.t1 AT5G27810 39.583 96 57 1 29 123 1 96 4.71e-19 77.8
MS.gene005758.t1 AT5G06500 35.135 148 90 3 1 145 1 145 6.43e-19 80.5
MS.gene005758.t1 AT5G26580 29.032 155 97 1 1 155 1 142 7.34e-16 73.6
MS.gene005758.t1 AT2G40210 32.857 140 88 3 1 137 1 137 2.61e-12 63.5

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACCTTCTTGGCTTCTTC+TGG 0.189716 1.2:+67913703 None:intergenic
ATACCTCTCAATTACCTTCT+TGG 0.215921 1.2:+67913693 None:intergenic
TGCCTTCCTTGCCGCGTTAT+CGG 0.274204 1.2:+67913849 None:intergenic
GACAACACTTTGTGATATTC+CGG 0.300516 1.2:-67913779 MS.gene005758:CDS
TTCTTGGCTTCTTCTGGATT+CGG 0.341741 1.2:+67913709 None:intergenic
TCTGCAGAATGTATCTTTCA+TGG 0.389169 1.2:+67913632 None:intergenic
TTGATTCTCAGACTGAGGTT+TGG 0.414602 1.2:-67913732 MS.gene005758:CDS
TGCCGCGTTATCGGAGATAA+AGG 0.416769 1.2:+67913858 None:intergenic
CTCCGATAACGCGGCAAGGA+AGG 0.449540 1.2:-67913851 MS.gene005758:CDS
AAGCTACAATAAAAGAAAGA+AGG 0.492405 1.2:-67913827 MS.gene005758:CDS
TTTCACCTTCCTTCTCGTCA+TGG 0.513068 1.2:+67913885 None:intergenic
ACCTCAGTCTGAGAATCAAA+AGG 0.514840 1.2:+67913736 None:intergenic
GCATGTTGAGAAGAAGTTGA+AGG 0.520532 1.2:-67913467 MS.gene005758:CDS
AGTCTGAGAATCAAAAGGAC+TGG 0.521393 1.2:+67913741 None:intergenic
TGCAGATGATTGCTAAAGCA+AGG 0.540172 1.2:-67913615 MS.gene005758:CDS
AAAGAAGGGTCTCATAAAGA+AGG 0.555375 1.2:-67913812 MS.gene005758:CDS
AGCTACAATAAAAGAAAGAA+GGG 0.567899 1.2:-67913826 MS.gene005758:CDS
TGTTGAGAAGAAGTTGAAGG+AGG 0.578028 1.2:-67913464 MS.gene005758:CDS
TGCAACCATGACGAGAAGGA+AGG 0.595441 1.2:-67913890 None:intergenic
GAATCCAGAAGAAGCCAAGA+AGG 0.598410 1.2:-67913707 MS.gene005758:CDS
TGCCTTTATCTCCGATAACG+CGG 0.615017 1.2:-67913860 MS.gene005758:CDS
TTATCTCCGATAACGCGGCA+AGG 0.616944 1.2:-67913855 MS.gene005758:CDS
CTGGAAATTATAGCACAAGC+CGG 0.619015 1.2:+67913760 None:intergenic
GCAGATGATTGCTAAAGCAA+GGG 0.625022 1.2:-67913614 MS.gene005758:CDS
AAGCCAAGAAGGTAATTGAG+AGG 0.625836 1.2:-67913696 MS.gene005758:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGCTACAATAAAAGAAAGA+AGG - chr1.2:67913491-67913510 MS.gene005758:CDS 25.0%
! AGCTACAATAAAAGAAAGAA+GGG - chr1.2:67913492-67913511 MS.gene005758:CDS 25.0%
AAAGAAGGGTCTCATAAAGA+AGG - chr1.2:67913506-67913525 MS.gene005758:CDS 35.0%
ATACCTCTCAATTACCTTCT+TGG + chr1.2:67913628-67913647 None:intergenic 35.0%
GACAACACTTTGTGATATTC+CGG - chr1.2:67913539-67913558 MS.gene005758:CDS 35.0%
TCTGCAGAATGTATCTTTCA+TGG + chr1.2:67913689-67913708 None:intergenic 35.0%
! AGCTGAATCTTTTTATGCTG+CGG - chr1.2:67913766-67913785 MS.gene005758:CDS 35.0%
AAGCCAAGAAGGTAATTGAG+AGG - chr1.2:67913622-67913641 MS.gene005758:CDS 40.0%
ACCTCAGTCTGAGAATCAAA+AGG + chr1.2:67913585-67913604 None:intergenic 40.0%
AGTCTGAGAATCAAAAGGAC+TGG + chr1.2:67913580-67913599 None:intergenic 40.0%
ATTACCTTCTTGGCTTCTTC+TGG + chr1.2:67913618-67913637 None:intergenic 40.0%
CTGGAAATTATAGCACAAGC+CGG + chr1.2:67913561-67913580 None:intergenic 40.0%
GCAGATGATTGCTAAAGCAA+GGG - chr1.2:67913704-67913723 MS.gene005758:CDS 40.0%
GCATGTTGAGAAGAAGTTGA+AGG - chr1.2:67913851-67913870 MS.gene005758:CDS 40.0%
TGCAGATGATTGCTAAAGCA+AGG - chr1.2:67913703-67913722 MS.gene005758:CDS 40.0%
TGTTGAGAAGAAGTTGAAGG+AGG - chr1.2:67913854-67913873 MS.gene005758:CDS 40.0%
TTCTTGGCTTCTTCTGGATT+CGG + chr1.2:67913612-67913631 None:intergenic 40.0%
TTGATTCTCAGACTGAGGTT+TGG - chr1.2:67913586-67913605 MS.gene005758:CDS 40.0%
! TTTTCAGCTCTTCAGCAGTT+AGG + chr1.2:67913821-67913840 None:intergenic 40.0%
!! TCCTTTTGATTCTCAGACTG+AGG - chr1.2:67913581-67913600 MS.gene005758:CDS 40.0%
GAATCCAGAAGAAGCCAAGA+AGG - chr1.2:67913611-67913630 MS.gene005758:CDS 45.0%
TGCCTTTATCTCCGATAACG+CGG - chr1.2:67913458-67913477 MS.gene005758:CDS 45.0%
TGCCGCGTTATCGGAGATAA+AGG + chr1.2:67913463-67913482 None:intergenic 50.0%
TTATCTCCGATAACGCGGCA+AGG - chr1.2:67913463-67913482 MS.gene005758:CDS 50.0%
TGCCTTCCTTGCCGCGTTAT+CGG + chr1.2:67913472-67913491 None:intergenic 55.0%
CTCCGATAACGCGGCAAGGA+AGG - chr1.2:67913467-67913486 MS.gene005758:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 67913435 67913905 67913435 ID=MS.gene005758
chr1.2 mRNA 67913435 67913905 67913435 ID=MS.gene005758.t1;Parent=MS.gene005758
chr1.2 exon 67913435 67913905 67913435 ID=MS.gene005758.t1.exon1;Parent=MS.gene005758.t1
chr1.2 CDS 67913435 67913905 67913435 ID=cds.MS.gene005758.t1;Parent=MS.gene005758.t1
Gene Sequence

>MS.gene005758

ATGACGAGAAGGAAGGTGAAACTTGCCTTTATCTCCGATAACGCGGCAAGGAAGGCAAGCTACAATAAAAGAAAGAAGGGTCTCATAAAGAAGGTAACTGAACTGACAACACTTTGTGATATTCCGGCTTGTGCTATAATTTCCAGTCCTTTTGATTCTCAGACTGAGGTTTGGCCGAATCCAGAAGAAGCCAAGAAGGTAATTGAGAGGTATCAAAGTTATTCTTTGATAGATGAAAACAAGAATGTGAGCCATGAAAGATACATTCTGCAGATGATTGCTAAAGCAAGGGACAAACTAAGAAAGTTGAAGCATGAGAATCGTGAGCAAGAGCTGAATCTTTTTATGCTGCGGAACTTGAAAAATAACAATTTGTATGAAGACCTAACTGCTGAAGAGCTGAAAAATCTTGCTAAGCATGTTGAGAAGAAGTTGAAGGAGGTTGATGACAAGATTGATACACTTGATTGA
Protein sequence

>MS.gene005758.t1

MTRRKVKLAFISDNAARKASYNKRKKGLIKKVTELTTLCDIPACAIISSPFDSQTEVWPNPEEAKKVIERYQSYSLIDENKNVSHERYILQMIAKARDKLRKLKHENREQELNLFMLRNLKNNNLYEDLTAEELKNLAKHVEKKLKEVDDKIDTLD