AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005866

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005866.t1 MTR_1g093080 96.825 378 12 0 1 378 1 378 0.0 762
MS.gene005866.t1 MTR_1g093080 96.935 261 8 0 1 261 1 261 0.0 528
MS.gene005866.t1 MTR_1g093080 96.610 236 8 0 1 236 1 236 2.53e-171 478
MS.gene005866.t1 MTR_5g054300 57.105 380 127 14 1 374 10 359 1.81e-124 363
MS.gene005866.t1 MTR_4g113140 35.404 322 144 7 2 294 29 315 4.22e-48 169
MS.gene005866.t1 MTR_4g086835 34.930 355 146 11 10 309 44 368 1.61e-42 153
MS.gene005866.t1 MTR_4g086835 34.930 355 145 12 10 309 44 367 9.84e-41 149
MS.gene005866.t1 MTR_5g017980 33.333 330 143 12 2 324 35 294 1.19e-33 127
MS.gene005866.t1 MTR_5g017980 33.435 329 142 12 2 324 35 292 3.74e-33 126
MS.gene005866.t1 MTR_5g017980 33.333 330 142 13 2 324 35 293 6.80e-32 123
MS.gene005866.t1 MTR_7g115530 34.437 151 89 3 162 304 161 309 1.42e-15 77.8
MS.gene005866.t1 MTR_2g027860 56.061 66 29 0 211 276 49 114 1.60e-15 77.0
MS.gene005866.t1 MTR_2g034960 37.008 127 57 4 210 323 210 326 3.24e-15 77.4
MS.gene005866.t1 MTR_4g121020 53.521 71 32 1 210 280 110 179 9.67e-15 75.9
MS.gene005866.t1 MTR_2g450070 38.462 117 63 4 209 323 211 320 1.01e-14 76.3
MS.gene005866.t1 MTR_4g121020 53.521 71 32 1 210 280 214 283 1.02e-14 76.3
MS.gene005866.t1 MTR_4g121020 53.521 71 32 1 210 280 214 283 1.07e-14 75.9
MS.gene005866.t1 MTR_2g450070 38.462 117 63 4 209 323 211 320 1.08e-14 75.9
MS.gene005866.t1 MTR_7g088070 54.167 72 31 1 211 280 50 121 1.40e-14 73.6
MS.gene005866.t1 MTR_7g088070 55.385 65 29 0 211 275 50 114 1.73e-14 73.6
MS.gene005866.t1 MTR_4g131580 53.968 63 28 1 209 271 198 259 2.56e-14 74.7
MS.gene005866.t1 MTR_1g032570 48.611 72 36 1 200 271 120 190 3.45e-14 74.3
MS.gene005866.t1 MTR_1g032570 48.611 72 36 1 200 271 189 259 4.87e-14 73.9
MS.gene005866.t1 MTR_3g102600 43.038 79 43 2 215 292 87 164 5.17e-14 70.1
MS.gene005866.t1 MTR_3g106220 45.679 81 42 2 204 284 191 269 5.93e-14 73.6
MS.gene005866.t1 MTR_7g115530 37.815 119 66 2 162 272 161 279 7.17e-14 72.0
MS.gene005866.t1 MTR_7g117705 48.611 72 36 1 209 280 189 259 8.31e-14 72.8
MS.gene005866.t1 MTR_5g014040 51.515 66 31 1 204 269 304 368 9.99e-14 72.8
MS.gene005866.t1 MTR_3g102600 43.038 79 43 2 215 292 136 213 1.24e-13 70.1
MS.gene005866.t1 MTR_7g115530 37.815 119 66 2 162 272 161 279 1.29e-13 71.6
MS.gene005866.t1 MTR_4g131570 50.000 66 32 1 206 271 198 262 1.40e-13 72.4
MS.gene005866.t1 MTR_5g041350 61.818 55 21 0 211 265 15 69 1.45e-13 71.6
MS.gene005866.t1 MTR_7g115530 54.839 62 28 0 211 272 218 279 2.93e-13 71.2
MS.gene005866.t1 MTR_4g098870 41.489 94 51 2 178 268 274 366 3.04e-13 71.2
MS.gene005866.t1 MTR_1g013170 52.381 63 29 1 209 271 189 250 3.21e-13 71.2
MS.gene005866.t1 MTR_4g098870 41.489 94 51 2 178 268 294 386 3.36e-13 71.2
MS.gene005866.t1 MTR_4g098870 41.489 94 51 2 178 268 274 366 3.54e-13 71.2
MS.gene005866.t1 MTR_4g098870 41.489 94 51 2 178 268 313 405 3.59e-13 71.2
MS.gene005866.t1 MTR_4g098870 41.489 94 51 2 178 268 294 386 3.66e-13 71.2
MS.gene005866.t1 MTR_7g098250 43.902 82 44 1 211 292 262 341 3.78e-13 70.9
MS.gene005866.t1 MTR_2g027800 60.000 55 22 0 211 265 39 93 5.82e-13 69.3
MS.gene005866.t1 MTR_1g080330 50.794 63 31 0 211 273 175 237 6.43e-13 68.9
MS.gene005866.t1 MTR_2g027800 60.000 55 22 0 211 265 39 93 8.43e-13 68.9
MS.gene005866.t1 MTR_1g080330 50.794 63 31 0 211 273 175 237 1.08e-12 69.3
MS.gene005866.t1 MTR_4g064730 43.243 74 41 1 207 280 127 199 1.23e-12 68.6
MS.gene005866.t1 MTR_6g444980 60.000 55 22 0 211 265 35 89 1.57e-12 68.6
MS.gene005866.t1 MTR_3g102590 43.590 78 41 2 209 284 141 217 1.62e-12 67.0
MS.gene005866.t1 MTR_6g444980 60.000 55 22 0 211 265 35 89 1.67e-12 68.2
MS.gene005866.t1 MTR_6g032990 48.529 68 35 0 211 278 42 109 1.69e-12 68.6
MS.gene005866.t1 MTR_4g081710 58.182 55 23 0 211 265 17 71 1.75e-12 68.2
MS.gene005866.t1 MTR_7g089010 40.244 82 45 2 188 269 71 148 1.84e-12 67.8
MS.gene005866.t1 MTR_1g053835 51.667 60 29 0 211 270 258 317 1.91e-12 68.6
MS.gene005866.t1 MTR_2g086450 56.364 55 24 0 211 265 45 99 1.98e-12 68.6
MS.gene005866.t1 MTR_1g053830 49.057 53 27 0 213 265 20 72 2.20e-12 63.2
MS.gene005866.t1 MTR_4g078810 37.895 95 46 2 209 303 156 237 3.47e-12 67.8
MS.gene005866.t1 MTR_4g021790 47.222 72 37 1 200 271 157 227 4.58e-12 66.2
MS.gene005866.t1 MTR_1g090670 58.182 55 23 0 211 265 44 98 5.13e-12 66.2
MS.gene005866.t1 MTR_2g090675 45.070 71 38 1 209 279 159 228 6.65e-12 67.0
MS.gene005866.t1 MTR_8g079940 46.479 71 37 1 210 280 208 277 7.25e-12 67.0
MS.gene005866.t1 MTR_5g014040 47.887 71 31 2 204 269 264 333 8.01e-12 67.0
MS.gene005866.t1 MTR_4g131600 46.970 66 33 2 204 268 193 257 8.91e-12 67.0
MS.gene005866.t1 MTR_5g014040 47.887 71 31 2 204 269 304 373 9.44e-12 66.6
MS.gene005866.t1 MTR_2g023580 55.769 52 23 0 213 264 57 108 1.39e-11 64.3
MS.gene005866.t1 MTR_7g093030 46.269 67 36 0 211 277 212 278 2.02e-11 64.7
MS.gene005866.t1 MTR_3g086100 40.698 86 45 2 200 280 112 196 2.10e-11 65.5
MS.gene005866.t1 MTR_5g027440 50.820 61 28 1 211 269 25 85 2.10e-11 64.3
MS.gene005866.t1 MTR_7g115530 37.615 109 60 2 162 262 161 269 2.13e-11 64.3
MS.gene005866.t1 MTR_0450s0040 43.243 74 41 1 210 283 168 240 2.89e-11 64.7
MS.gene005866.t1 MTR_3g086100 40.698 86 45 2 200 280 183 267 3.60e-11 65.1
MS.gene005866.t1 MTR_7g115530 31.852 135 83 3 162 288 161 294 3.83e-11 63.9
MS.gene005866.t1 MTR_7g093030 46.269 67 36 0 211 277 212 278 5.30e-11 63.9
MS.gene005866.t1 MTR_7g026400 49.153 59 29 1 210 268 156 213 6.30e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005866.t1 AT1G68670 47.633 338 127 12 10 326 16 324 1.55e-78 246
MS.gene005866.t1 AT1G25550 46.084 332 126 11 10 328 20 311 1.48e-76 240
MS.gene005866.t1 AT3G25790 43.597 367 167 8 10 372 20 350 2.37e-74 235
MS.gene005866.t1 AT1G13300 42.935 368 149 12 11 368 20 336 6.86e-70 223
MS.gene005866.t1 AT1G25550 48.846 260 105 8 10 261 20 259 9.72e-66 210
MS.gene005866.t1 AT3G25790 46.063 254 110 4 9 261 19 246 8.24e-56 184
MS.gene005866.t1 AT2G03500 35.494 324 127 9 8 294 36 314 1.41e-47 167
MS.gene005866.t1 AT4G37180 33.935 277 109 8 11 269 48 268 5.13e-33 127
MS.gene005866.t1 AT4G37180 33.574 277 117 8 11 269 48 275 2.24e-30 119
MS.gene005866.t1 AT1G49560 32.542 295 132 9 10 293 30 268 1.03e-29 117
MS.gene005866.t1 AT4G37180 33.086 269 106 8 11 261 48 260 2.86e-28 112
MS.gene005866.t1 AT1G49560 31.939 263 118 8 10 261 30 242 8.43e-23 96.7
MS.gene005866.t1 AT2G01060 57.812 64 27 0 211 274 15 78 2.15e-15 76.3
MS.gene005866.t1 AT3G16857 35.252 139 79 3 199 336 155 283 8.27e-15 76.3
MS.gene005866.t1 AT3G16857 34.752 141 81 3 199 338 225 355 1.01e-14 76.3
MS.gene005866.t1 AT3G16857 34.752 141 81 3 199 338 225 355 1.11e-14 75.9
MS.gene005866.t1 AT5G58080 50.000 66 32 1 204 269 187 251 4.26e-14 74.3
MS.gene005866.t1 AT3G04030 53.846 65 30 0 211 275 45 109 1.03e-13 72.4
MS.gene005866.t1 AT2G25180 31.902 163 94 5 209 356 193 353 1.06e-13 72.8
MS.gene005866.t1 AT3G04030 53.846 65 30 0 211 275 45 109 1.18e-13 72.0
MS.gene005866.t1 AT5G05090 45.570 79 41 2 192 269 61 138 1.99e-13 70.1
MS.gene005866.t1 AT5G06800 53.226 62 29 0 211 272 192 253 2.99e-13 70.1
MS.gene005866.t1 AT5G18240 58.182 55 23 0 211 265 45 99 3.93e-13 70.5
MS.gene005866.t1 AT5G18240 58.182 55 23 0 211 265 45 99 3.93e-13 70.5
MS.gene005866.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.66e-13 70.5
MS.gene005866.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.78e-13 70.5
MS.gene005866.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.78e-13 70.5
MS.gene005866.t1 AT2G01760 44.156 77 42 1 210 286 134 209 4.99e-13 69.7
MS.gene005866.t1 AT5G44190 43.750 80 44 1 200 279 136 214 6.11e-13 69.7
MS.gene005866.t1 AT5G44190 43.750 80 44 1 200 279 136 214 6.68e-13 69.7
MS.gene005866.t1 AT2G01760 44.156 77 42 1 210 286 227 302 7.08e-13 70.1
MS.gene005866.t1 AT2G01760 44.156 77 42 1 210 286 199 274 7.53e-13 69.7
MS.gene005866.t1 AT5G06800 53.226 62 29 0 211 272 192 253 9.76e-13 69.3
MS.gene005866.t1 AT3G10760 44.444 81 43 2 190 269 83 162 1.03e-12 68.9
MS.gene005866.t1 AT5G06800 53.226 62 29 0 211 272 192 253 1.06e-12 69.3
MS.gene005866.t1 AT2G40970 45.070 71 38 1 199 269 93 162 1.18e-12 67.8
MS.gene005866.t1 AT3G46640 33.113 151 92 4 134 276 59 208 1.31e-12 68.6
MS.gene005866.t1 AT3G46640 33.113 151 92 4 134 276 59 208 1.53e-12 68.2
MS.gene005866.t1 AT3G46640 33.113 151 92 4 134 276 59 208 1.53e-12 68.2
MS.gene005866.t1 AT5G45580 44.595 74 41 0 211 284 23 96 1.60e-12 67.8
MS.gene005866.t1 AT5G45580 44.595 74 41 0 211 284 7 80 1.62e-12 67.4
MS.gene005866.t1 AT1G79430 60.000 55 22 0 211 265 34 88 1.70e-12 68.6
MS.gene005866.t1 AT3G04450 51.667 60 29 0 211 270 238 297 1.84e-12 67.8
MS.gene005866.t1 AT5G59570 38.318 107 64 2 163 269 94 198 2.48e-12 67.4
MS.gene005866.t1 AT5G59570 38.318 107 64 2 163 269 94 198 2.48e-12 67.4
MS.gene005866.t1 AT3G24120 58.182 55 23 0 211 265 41 95 2.70e-12 67.4
MS.gene005866.t1 AT5G29000 49.206 63 32 0 211 273 231 293 2.71e-12 67.4
MS.gene005866.t1 AT4G13640 58.182 55 23 0 211 265 37 91 2.89e-12 67.0
MS.gene005866.t1 AT3G24120 58.182 55 23 0 211 265 41 95 2.91e-12 67.4
MS.gene005866.t1 AT4G13640 58.182 55 23 0 211 265 37 91 2.97e-12 67.0
MS.gene005866.t1 AT3G13040 52.459 61 29 0 210 270 240 300 3.27e-12 68.2
MS.gene005866.t1 AT3G13040 52.459 61 29 0 210 270 240 300 3.27e-12 68.2
MS.gene005866.t1 AT3G13040 52.459 61 29 0 210 270 240 300 3.27e-12 68.2
MS.gene005866.t1 AT3G04450 51.667 60 29 0 211 270 238 297 3.58e-12 67.8
MS.gene005866.t1 AT3G04450 51.667 60 29 0 211 270 198 257 3.85e-12 67.8
MS.gene005866.t1 AT3G04450 51.667 60 29 0 211 270 198 257 3.85e-12 67.8
MS.gene005866.t1 AT2G20570 47.945 73 37 1 207 279 149 220 4.38e-12 67.4
MS.gene005866.t1 AT5G29000 49.206 63 32 0 211 273 231 293 6.22e-12 67.0
MS.gene005866.t1 AT5G29000 49.206 63 32 0 211 273 188 250 6.70e-12 66.6
MS.gene005866.t1 AT5G29000 49.206 63 32 0 211 273 188 250 6.70e-12 66.6
MS.gene005866.t1 AT3G04030 52.308 65 30 1 211 275 45 108 6.72e-12 67.0
MS.gene005866.t1 AT4G28610 51.667 60 29 0 211 270 225 284 6.72e-12 67.0
MS.gene005866.t1 AT3G12730 56.364 55 24 0 211 265 23 77 8.38e-12 65.1
MS.gene005866.t1 AT2G42660 52.632 57 27 0 216 272 55 111 1.15e-11 65.1
MS.gene005866.t1 AT2G38300 51.923 52 25 0 213 264 56 107 1.94e-11 65.1
MS.gene005866.t1 AT4G31920 42.105 76 43 1 204 279 176 250 2.34e-11 65.5
MS.gene005866.t1 AT2G06020 50.909 55 27 0 211 265 86 140 2.62e-11 64.3
MS.gene005866.t1 AT1G49190 36.486 74 44 1 196 266 200 273 6.08e-11 63.9
MS.gene005866.t1 AT4G04605 51.923 52 25 0 213 264 16 67 7.85e-11 62.0

Find 78 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAACTCTATCTTCTTTA+TGG 0.063110 1.1:+67521296 None:intergenic
CACAATGCAGCGTTTCAAAA+TGG 0.179894 1.1:-67522033 None:intergenic
ACAAAAGTGGTTGCACCTTC+TGG 0.247597 1.1:-67520480 MS.gene005866:CDS
CATATCTACAAACCTGTAAA+TGG 0.279375 1.1:+67520887 None:intergenic
TGAAGTCAAAAGCCATTTAC+AGG 0.284068 1.1:-67520899 MS.gene005866:intron
AACGAAAGAGGAAGTTCTTT+AGG 0.297245 1.1:+67521954 None:intergenic
GATGTCTCCAACATTGTTAC+TGG 0.321828 1.1:+67521192 None:intergenic
ATTACCTCTCTGATTTGCTT+TGG 0.356836 1.1:+67520946 None:intergenic
CTTTCGTTATTTGTTGAACT+TGG 0.382926 1.1:+67520606 None:intergenic
GGCTTAGATCTGTTCAGTTA+TGG 0.389861 1.1:-67521556 MS.gene005866:CDS
CCCCACCATACTCTTGTTGT+TGG 0.395076 1.1:+67520532 None:intergenic
GGAATAAACTCTTCTAAAAC+AGG 0.409660 1.1:+67521698 None:intergenic
TTTGTCCAACAACAAGAGTA+TGG 0.414336 1.1:-67520537 MS.gene005866:CDS
ATTATTACCTCTTCAGATGA+AGG 0.417768 1.1:+67521524 None:intergenic
GACGTTCTTGTTCTAATGCT+TGG 0.420567 1.1:+67521994 None:intergenic
ACAATGCAGCGTTTCAAAAT+GGG 0.423030 1.1:-67522032 MS.gene005866:CDS
GTAGCTTCATCAACAACAAC+AGG 0.429695 1.1:-67520510 MS.gene005866:CDS
TGCTCAAACCTCTTGAACTC+TGG 0.432408 1.1:+67520408 None:intergenic
GCAGCGACGGTGCTGGTCGC+AGG 0.434166 1.1:-67521140 MS.gene005866:CDS
GTTTATTCCAATTAAGAAAA+GGG 0.437351 1.1:-67521684 MS.gene005866:CDS
GTTGCACAAGCGATTCTTGA+AGG 0.440339 1.1:-67521116 MS.gene005866:CDS
AGTTTATTCCAATTAAGAAA+AGG 0.446516 1.1:-67521685 MS.gene005866:CDS
ACTCTATCTTCTTTATGGAA+TGG 0.449526 1.1:+67521301 None:intergenic
GCTTGTCGACAACAATTGTC+TGG 0.459321 1.1:-67521788 MS.gene005866:CDS
CATCAAAAGAGACGAGAAAG+AGG 0.461312 1.1:-67521173 MS.gene005866:CDS
TTGAAGGCGCTTCAGCAACT+TGG 0.469776 1.1:-67521100 MS.gene005866:CDS
AGAGAGGTAATGAATGTTGA+TGG 0.478733 1.1:-67520934 MS.gene005866:CDS
GCATTCAATCTCAGATGAAA+GGG 0.480171 1.1:-67520388 MS.gene005866:CDS
AGGTGCTGCTGTTTGTGAAT+TGG 0.480961 1.1:+67520581 None:intergenic
CTCTCTGATTTGCTTTGGTG+TGG 0.488332 1.1:+67520951 None:intergenic
GTGTTGGAATTGAAGAGAAA+TGG 0.488831 1.1:-67521346 MS.gene005866:CDS
ACAACTGAGTATAATTTGAA+TGG 0.489356 1.1:-67521761 MS.gene005866:CDS
TCAATAACCAGGGGAAGTAT+TGG 0.491273 1.1:+67520299 None:intergenic
TTGGTCTTCTAGTATGCAAC+CGG 0.498211 1.1:+67520625 None:intergenic
ACAAGTGAATTGTTAAGTAA+AGG 0.498649 1.1:-67521268 MS.gene005866:CDS
ATGTTGCCTACAAGGACAAA+AGG 0.503411 1.1:+67520561 None:intergenic
AAGGCGCTTCAGCAACTTGG+TGG 0.504237 1.1:-67521097 MS.gene005866:CDS
ACTTGAGGATGTGTGGCTAC+TGG 0.518302 1.1:+67520447 None:intergenic
TTCTTAATTGTAGCAACTTG+AGG 0.518381 1.1:+67520432 None:intergenic
AGCATTCAATCTCAGATGAA+AGG 0.522044 1.1:-67520389 MS.gene005866:CDS
AAGAGAAATGGAAGTGGTGG+TGG 0.523432 1.1:-67521334 MS.gene005866:CDS
TGTGTAGAGGATGAAGATGT+AGG 0.529070 1.1:+67520330 None:intergenic
TTAAGAAACCAGAGTTCAAG+AGG 0.534779 1.1:-67520416 MS.gene005866:CDS
TCCAACAACAAGAGTATGGT+GGG 0.538170 1.1:-67520533 MS.gene005866:CDS
TGAACAGATCTAAGCCAGTC+AGG 0.539679 1.1:+67521563 None:intergenic
AGAGAAAGCAGCGACGGTGC+TGG 0.540249 1.1:-67521147 MS.gene005866:CDS
AGAACTCACGGCAGCTACAC+CGG 0.545612 1.1:+67521231 None:intergenic
GTAACGAGTTCTAACGAAAG+AGG 0.565892 1.1:+67521942 None:intergenic
GGCAGCTACACCGGTCGAAG+AGG 0.574491 1.1:+67521240 None:intergenic
AAAATTAAAACTCAATAACC+AGG 0.575545 1.1:+67520288 None:intergenic
ATAATAAGAAGAAACCTGAC+TGG 0.575850 1.1:-67521577 MS.gene005866:CDS
AGGAAAAGAGAAAGCAGCGA+CGG 0.577313 1.1:-67521153 MS.gene005866:CDS
ACTACTACCAATACTTCCCC+TGG 0.577357 1.1:-67520306 MS.gene005866:CDS
TAAGAAAACAGTGCCTGTGT+TGG 0.581215 1.1:-67521362 MS.gene005866:CDS
TTCAGATCCTTCATCTGAAG+AGG 0.584122 1.1:-67521531 MS.gene005866:intron
AAAGGACAACCCTCTTCGAC+CGG 0.587220 1.1:-67521250 MS.gene005866:CDS
AAATTAAAACTCAATAACCA+GGG 0.590216 1.1:+67520289 None:intergenic
GCAGCTACACCGGTCGAAGA+GGG 0.597437 1.1:+67521241 None:intergenic
TTTCTCTTCAATTCCAACAC+AGG 0.600323 1.1:+67521349 None:intergenic
AACAGGGGAAATGACAAAAG+TGG 0.604173 1.1:-67520493 MS.gene005866:CDS
ATTGGTAGTAGTATGTGTAG+AGG 0.610237 1.1:+67520317 None:intergenic
AATGTCACCAGTAACAATGT+TGG 0.610482 1.1:-67521199 MS.gene005866:CDS
GTCCAACAACAAGAGTATGG+TGG 0.618792 1.1:-67520534 MS.gene005866:CDS
GAACAGACAACATCAACAGA+TGG 0.623810 1.1:-67521722 MS.gene005866:CDS
ATTAGCCGCATTAGAACTCA+CGG 0.629576 1.1:+67521219 None:intergenic
TCGTTAGAACTCGTTACACA+AGG 0.638093 1.1:-67521936 MS.gene005866:intron
GAAAGGGGTAATAACAGTGA+AGG 0.640994 1.1:-67520372 MS.gene005866:CDS
TTGAAGAGAAATGGAAGTGG+TGG 0.651388 1.1:-67521337 MS.gene005866:CDS
CATTCAATCTCAGATGAAAG+GGG 0.655574 1.1:-67520387 MS.gene005866:CDS
ACTGGTGCATAGATTCCAGA+AGG 0.656305 1.1:+67520465 None:intergenic
AGCTGCCGTGAGTTCTAATG+CGG 0.671481 1.1:-67521224 MS.gene005866:CDS
TGTAGCAACTTGAGGATGTG+TGG 0.683531 1.1:+67520440 None:intergenic
GAATTGAAGAGAAATGGAAG+TGG 0.689165 1.1:-67521340 MS.gene005866:CDS
TAGCTTCATCAACAACAACA+GGG 0.693117 1.1:-67520509 MS.gene005866:CDS
AATTAAAACTCAATAACCAG+GGG 0.700665 1.1:+67520290 None:intergenic
CCAACAACAAGAGTATGGTG+GGG 0.702776 1.1:-67520532 MS.gene005866:CDS
AGCTTCATCAACAACAACAG+GGG 0.709783 1.1:-67520508 MS.gene005866:CDS
CACACCAAAGCAAATCAGAG+AGG 0.720773 1.1:-67520950 MS.gene005866:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATATAAAAAAAACAAAA+AGG + chr1.1:67520429-67520448 None:intergenic 10.0%
!! TCATATAAAAAAAACAAAAA+GGG + chr1.1:67520428-67520447 None:intergenic 10.0%
!!! AAAAAAATTGATTTTAATTG+AGG + chr1.1:67521659-67521678 None:intergenic 10.0%
!!! AAAATAATTTTGGATTTTTT+TGG - chr1.1:67520498-67520517 MS.gene005866:CDS 10.0%
!!! AAGTTTGTATTTTTATTATC+TGG - chr1.1:67520458-67520477 MS.gene005866:CDS 15.0%
!!! AGTTTGTATTTTTATTATCT+GGG - chr1.1:67520459-67520478 MS.gene005866:CDS 15.0%
!!! TTACTTTTTGTTAAACTAAA+GGG - chr1.1:67520876-67520895 MS.gene005866:intron 15.0%
!!! TTTACTTTTTGTTAAACTAA+AGG - chr1.1:67520875-67520894 MS.gene005866:intron 15.0%
!!! TTTTTTGTGAAAAATTGTTT+AGG - chr1.1:67520936-67520955 MS.gene005866:CDS 15.0%
!! AGTTTATTCCAATTAAGAAA+AGG - chr1.1:67520643-67520662 MS.gene005866:intron 20.0%
!! GTTTATTCCAATTAAGAAAA+GGG - chr1.1:67520644-67520663 MS.gene005866:intron 20.0%
!!! TTTTCACAAAAAACAAAACA+AGG + chr1.1:67520929-67520948 None:intergenic 20.0%
! AAAATTGACAACAAAAGAGT+TGG + chr1.1:67521476-67521495 None:intergenic 25.0%
! AAATTGACAACAAAAGAGTT+GGG + chr1.1:67521475-67521494 None:intergenic 25.0%
! ACAACTGAGTATAATTTGAA+TGG - chr1.1:67520567-67520586 MS.gene005866:CDS 25.0%
! ACAAGTGAATTGTTAAGTAA+AGG - chr1.1:67521060-67521079 MS.gene005866:intron 25.0%
! TGTTAACTCTATCTTCTTTA+TGG + chr1.1:67521035-67521054 None:intergenic 25.0%
ACTCTATCTTCTTTATGGAA+TGG + chr1.1:67521030-67521049 None:intergenic 30.0%
ATAATAAGAAGAAACCTGAC+TGG - chr1.1:67520751-67520770 MS.gene005866:intron 30.0%
ATTATTACCTCTTCAGATGA+AGG + chr1.1:67520807-67520826 None:intergenic 30.0%
CATATCTACAAACCTGTAAA+TGG + chr1.1:67521444-67521463 None:intergenic 30.0%
GAAATCCTATGCAATTGAAA+GGG + chr1.1:67521580-67521599 None:intergenic 30.0%
TGAAATCCTATGCAATTGAA+AGG + chr1.1:67521581-67521600 None:intergenic 30.0%
TTCTTAATTGTAGCAACTTG+AGG + chr1.1:67521899-67521918 None:intergenic 30.0%
! CTTTCGTTATTTGTTGAACT+TGG + chr1.1:67521725-67521744 None:intergenic 30.0%
!! GGAATAAACTCTTCTAAAAC+AGG + chr1.1:67520633-67520652 None:intergenic 30.0%
!!! GTTTTTTGCTTCTGTTCTAT+AGG - chr1.1:67521352-67521371 MS.gene005866:CDS 30.0%
!!! TTTTGACTTCATCATTTGTG+AGG + chr1.1:67521420-67521439 None:intergenic 30.0%
AACGAAAGAGGAAGTTCTTT+AGG + chr1.1:67520377-67520396 None:intergenic 35.0%
AATGTCACCAGTAACAATGT+TGG - chr1.1:67521129-67521148 MS.gene005866:CDS 35.0%
AGAGAGGTAATGAATGTTGA+TGG - chr1.1:67521394-67521413 MS.gene005866:intron 35.0%
AGCATTCAATCTCAGATGAA+AGG - chr1.1:67521939-67521958 MS.gene005866:CDS 35.0%
CATTCAATCTCAGATGAAAG+GGG - chr1.1:67521941-67521960 MS.gene005866:CDS 35.0%
GAATTGAAGAGAAATGGAAG+TGG - chr1.1:67520988-67521007 MS.gene005866:intron 35.0%
GCATTCAATCTCAGATGAAA+GGG - chr1.1:67521940-67521959 MS.gene005866:CDS 35.0%
GTGTTGGAATTGAAGAGAAA+TGG - chr1.1:67520982-67521001 MS.gene005866:intron 35.0%
TAGCTTCATCAACAACAACA+GGG - chr1.1:67521819-67521838 MS.gene005866:intron 35.0%
TGAAGTCAAAAGCCATTTAC+AGG - chr1.1:67521429-67521448 MS.gene005866:intron 35.0%
TTAAGAAACCAGAGTTCAAG+AGG - chr1.1:67521912-67521931 MS.gene005866:intron 35.0%
TTAGTCCCTTTCAATTGCAT+AGG - chr1.1:67521572-67521591 MS.gene005866:CDS 35.0%
TTTCTCTTCAATTCCAACAC+AGG + chr1.1:67520982-67521001 None:intergenic 35.0%
TTTGTCCAACAACAAGAGTA+TGG - chr1.1:67521791-67521810 MS.gene005866:CDS 35.0%
! ATTGGTAGTAGTATGTGTAG+AGG + chr1.1:67522014-67522033 None:intergenic 35.0%
! GAAGAAGCCCTTTTCTTAAT+TGG + chr1.1:67520654-67520673 None:intergenic 35.0%
! TCTGTTTTGCACAGAAATAC+CGG - chr1.1:67521684-67521703 MS.gene005866:CDS 35.0%
!! ATTACCTCTCTGATTTGCTT+TGG + chr1.1:67521385-67521404 None:intergenic 35.0%
AACAGGGGAAATGACAAAAG+TGG - chr1.1:67521835-67521854 MS.gene005866:intron 40.0%
AGCTTCATCAACAACAACAG+GGG - chr1.1:67521820-67521839 MS.gene005866:intron 40.0%
ATGTTGCCTACAAGGACAAA+AGG + chr1.1:67521770-67521789 None:intergenic 40.0%
ATTAGCCGCATTAGAACTCA+CGG + chr1.1:67521112-67521131 None:intergenic 40.0%
CATCAAAAGAGACGAGAAAG+AGG - chr1.1:67521155-67521174 MS.gene005866:CDS 40.0%
GAAAGGGGTAATAACAGTGA+AGG - chr1.1:67521956-67521975 MS.gene005866:CDS 40.0%
GAACAGACAACATCAACAGA+TGG - chr1.1:67520606-67520625 MS.gene005866:CDS 40.0%
GACGTTCTTGTTCTAATGCT+TGG + chr1.1:67520337-67520356 None:intergenic 40.0%
GATGTCTCCAACATTGTTAC+TGG + chr1.1:67521139-67521158 None:intergenic 40.0%
GGACAAAAATGTTGCCTACA+AGG + chr1.1:67521778-67521797 None:intergenic 40.0%
GGCTTAGATCTGTTCAGTTA+TGG - chr1.1:67520772-67520791 MS.gene005866:intron 40.0%
GTAGCTTCATCAACAACAAC+AGG - chr1.1:67521818-67521837 MS.gene005866:intron 40.0%
TAAGAAAACAGTGCCTGTGT+TGG - chr1.1:67520966-67520985 MS.gene005866:intron 40.0%
TCGTTAGAACTCGTTACACA+AGG - chr1.1:67520392-67520411 MS.gene005866:CDS 40.0%
TGTGTAGAGGATGAAGATGT+AGG + chr1.1:67522001-67522020 None:intergenic 40.0%
TTCAGATCCTTCATCTGAAG+AGG - chr1.1:67520797-67520816 MS.gene005866:intron 40.0%
TTGAAGAGAAATGGAAGTGG+TGG - chr1.1:67520991-67521010 MS.gene005866:intron 40.0%
! GTAACGAGTTCTAACGAAAG+AGG + chr1.1:67520389-67520408 None:intergenic 40.0%
! TCAATAACCAGGGGAAGTAT+TGG + chr1.1:67522032-67522051 None:intergenic 40.0%
! TCCAACAACAAGAGTATGGT+GGG - chr1.1:67521795-67521814 MS.gene005866:CDS 40.0%
! TTGGTCTTCTAGTATGCAAC+CGG + chr1.1:67521706-67521725 None:intergenic 40.0%
AAGAGAAATGGAAGTGGTGG+TGG - chr1.1:67520994-67521013 MS.gene005866:intron 45.0%
ACAAAAGTGGTTGCACCTTC+TGG - chr1.1:67521848-67521867 MS.gene005866:intron 45.0%
ACTACTACCAATACTTCCCC+TGG - chr1.1:67522022-67522041 MS.gene005866:CDS 45.0%
ACTGGTGCATAGATTCCAGA+AGG + chr1.1:67521866-67521885 None:intergenic 45.0%
AGGAAAAGAGAAAGCAGCGA+CGG - chr1.1:67521175-67521194 MS.gene005866:CDS 45.0%
CACACCAAAGCAAATCAGAG+AGG - chr1.1:67521378-67521397 MS.gene005866:intron 45.0%
GCTTGTCGACAACAATTGTC+TGG - chr1.1:67520540-67520559 MS.gene005866:CDS 45.0%
GTTGCACAAGCGATTCTTGA+AGG - chr1.1:67521212-67521231 MS.gene005866:CDS 45.0%
TGAACAGATCTAAGCCAGTC+AGG + chr1.1:67520768-67520787 None:intergenic 45.0%
TGCTCAAACCTCTTGAACTC+TGG + chr1.1:67521923-67521942 None:intergenic 45.0%
TGTAGCAACTTGAGGATGTG+TGG + chr1.1:67521891-67521910 None:intergenic 45.0%
! CCAACAACAAGAGTATGGTG+GGG - chr1.1:67521796-67521815 MS.gene005866:CDS 45.0%
! GTCCAACAACAAGAGTATGG+TGG - chr1.1:67521794-67521813 MS.gene005866:CDS 45.0%
!! AGGTGCTGCTGTTTGTGAAT+TGG + chr1.1:67521750-67521769 None:intergenic 45.0%
!! CTCTCTGATTTGCTTTGGTG+TGG + chr1.1:67521380-67521399 None:intergenic 45.0%
!!! TTATTTTTGAAAAATAATTT+TGG - chr1.1:67520488-67520507 MS.gene005866:CDS 5.0%
AAAGGACAACCCTCTTCGAC+CGG - chr1.1:67521078-67521097 MS.gene005866:intron 50.0%
ACTTGAGGATGTGTGGCTAC+TGG + chr1.1:67521884-67521903 None:intergenic 50.0%
! AGCTGCCGTGAGTTCTAATG+CGG - chr1.1:67521104-67521123 MS.gene005866:CDS 50.0%
! CCCCACCATACTCTTGTTGT+TGG + chr1.1:67521799-67521818 None:intergenic 50.0%
! GCAGCACCTTTTGTCCTTGT+AGG - chr1.1:67521761-67521780 MS.gene005866:CDS 50.0%
!! TTGAAGGCGCTTCAGCAACT+TGG - chr1.1:67521228-67521247 MS.gene005866:CDS 50.0%
AGAACTCACGGCAGCTACAC+CGG + chr1.1:67521100-67521119 None:intergenic 55.0%
! AAGGCGCTTCAGCAACTTGG+TGG - chr1.1:67521231-67521250 MS.gene005866:CDS 55.0%
AGAGAAAGCAGCGACGGTGC+TGG - chr1.1:67521181-67521200 MS.gene005866:CDS 60.0%
GCAGCTACACCGGTCGAAGA+GGG + chr1.1:67521090-67521109 None:intergenic 60.0%
GGCAGCTACACCGGTCGAAG+AGG + chr1.1:67521091-67521110 None:intergenic 65.0%
!! GCAGCGACGGTGCTGGTCGC+AGG - chr1.1:67521188-67521207 MS.gene005866:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 67520299 67522051 67520299 ID=MS.gene005866
chr1.1 mRNA 67520299 67522051 67520299 ID=MS.gene005866.t1;Parent=MS.gene005866
chr1.1 exon 67521937 67522051 67521937 ID=MS.gene005866.t1.exon1;Parent=MS.gene005866.t1
chr1.1 CDS 67521937 67522051 67521937 ID=cds.MS.gene005866.t1;Parent=MS.gene005866.t1
chr1.1 exon 67521532 67521818 67521532 ID=MS.gene005866.t1.exon2;Parent=MS.gene005866.t1
chr1.1 CDS 67521532 67521818 67521532 ID=cds.MS.gene005866.t1;Parent=MS.gene005866.t1
chr1.1 exon 67521089 67521392 67521089 ID=MS.gene005866.t1.exon3;Parent=MS.gene005866.t1
chr1.1 CDS 67521089 67521392 67521089 ID=cds.MS.gene005866.t1;Parent=MS.gene005866.t1
chr1.1 exon 67520900 67520976 67520900 ID=MS.gene005866.t1.exon4;Parent=MS.gene005866.t1
chr1.1 CDS 67520900 67520976 67520900 ID=cds.MS.gene005866.t1;Parent=MS.gene005866.t1
chr1.1 exon 67520299 67520652 67520299 ID=MS.gene005866.t1.exon5;Parent=MS.gene005866.t1
chr1.1 CDS 67520299 67520652 67520299 ID=cds.MS.gene005866.t1;Parent=MS.gene005866.t1
Gene Sequence

>MS.gene005866

ATGCAGCGTTTCAAAATGGGTTTCTCTGATTATATCCAAGCATTAGAACAAGAACGTCGTAAGATTCAAGTCTTTCCTAAAGAACTTCCTCTTTCGTTAGAACTCGTTACACAAGGTACGTACATACCCTTTTTGTTTTTTTTATATGATTATGAGTTAAAGTTTGTATTTTTATTATCTGGGTTTGTTTTATTTTTGAAAAATAATTTTGGATTTTTTTGGTTGATTATTAGCTATTGAAGCTTGTCGACAACAATTGTCTGGAACAACAACTGAGTATAATTTGAATGGACAATCTGAGTGTTCTGAACAGACAACATCAACAGATGGACCTGTTTTAGAAGAGTTTATTCCAATTAAGAAAAGGGCTTCTTCTTATTCTCAAGAAGTGTTTGATGATGTAGAAGATTATGATCAACATTTTCATCATAAGCAACAAAAACTTTCATTAGATAATAAGAAGAAACCTGACTGGCTTAGATCTGTTCAGTTATGGAATTCAGATCCTTCATCTGAAGAGGTAATAATAAAATCATAGCTTATTTTTCAATTATTGTTGATTGTTGTTTTAGTTTTTTTACTTTTTGTTAAACTAAAGGGAACAAACAATGAATTATAAGTAATTGACCTTGTTTTGTTTTTTGTGAAAAATTGTTTAGGATGTTACTAAGAAAACAGTGCCTGTGTTGGAATTGAAGAGAAATGGAAGTGGTGGTGGTGCTTTTCATCCATTCCATAAAGAAGATAGAGTTAACAACAAGACAAGTGAATTGTTAAGTAAAGGACAACCCTCTTCGACCGGTGTAGCTGCCGTGAGTTCTAATGCGGCTAATGTCACCAGTAACAATGTTGGAGACATCAAAAGAGACGAGAAAGAGGAAAAGAGAAAGCAGCGACGGTGCTGGTCGCAGGAGTTGCACAAGCGATTCTTGAAGGCGCTTCAGCAACTTGGTGGTGCAGATTGTGAGTATCTTAATATATACATCAAGTTCTGATTGTTATGTATTCATGTTGTTAATGTAATTAACTGCAAATAGAATAAATGTTTAATGTGTTTTTTGCTTCTGTTCTATAGGTGCCACACCAAAGCAAATCAGAGAGGTAATGAATGTTGATGGCCTCACAAATGATGAAGTCAAAAGCCATTTACAGGTTTGTAGATATGTTCTTCTTCCCAACTCTTTTGTTGTCAATTTTCATCTGAGTATTTGTTTTGATTTTGTGATTTCTATTATCTAGTTGAATCTTTTAGTTTTAACTGTTTCATCTTGTTTTAGTCCCTTTCAATTGCATAGGATTTCAAAACAAACGAGTGATAGTTTTTGTTGTTACAGAAGTTACAACAACAGTTTAATATCCTCAATTAAAATCAATTTTTTTTTCTTTCTGTTTTGCACAGAAATACCGGTTGCATACTAGAAGACCAAGTTCAACAAATAACGAAAGTGCCAATTCACAAACAGCAGCACCTTTTGTCCTTGTAGGCAACATTTTTGTCCAACAACAAGAGTATGGTGGGGTAGCTTCATCAACAACAACAGGGGAAATGACAAAAGTGGTTGCACCTTCTGGAATCTATGCACCAGTAGCCACACATCCTCAAGTTGCTACAATTAAGAAACCAGAGTTCAAGAGGTTTGAGCATTCAATCTCAGATGAAAGGGGTAATAACAGTGAAGGTGCTGTTCATTCAAATTCACCTACATCTTCATCCTCTACACATACTACTACCAATACTTCCCCTGGTTATTGA
Protein sequence

>MS.gene005866.t1

MQRFKMGFSDYIQALEQERRKIQVFPKELPLSLELVTQAIEACRQQLSGTTTEYNLNGQSECSEQTTSTDGPVLEEFIPIKKRASSYSQEVFDDVEDYDQHFHHKQQKLSLDNKKKPDWLRSVQLWNSDPSSEEDVTKKTVPVLELKRNGSGGGAFHPFHKEDRVNNKTSELLSKGQPSSTGVAAVSSNAANVTSNNVGDIKRDEKEEKRKQRRCWSQELHKRFLKALQQLGGADCATPKQIREVMNVDGLTNDEVKSHLQKYRLHTRRPSSTNNESANSQTAAPFVLVGNIFVQQQEYGGVASSTTTGEMTKVVAPSGIYAPVATHPQVATIKKPEFKRFEHSISDERGNNSEGAVHSNSPTSSSSTHTTTNTSPGY