AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005963

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005963.t1 MTR_1g094930 98.792 331 4 0 1 331 1 331 0.0 674
MS.gene005963.t1 MTR_1g094930 98.729 236 3 0 96 331 1 236 6.26e-174 482
MS.gene005963.t1 MTR_1g116010 56.202 258 77 10 74 329 22 245 2.72e-74 229
MS.gene005963.t1 MTR_2g094600 46.774 186 70 6 121 306 124 280 5.82e-43 150
MS.gene005963.t1 MTR_1g006650 34.783 322 133 11 9 306 30 298 4.51e-38 139
MS.gene005963.t1 MTR_3g109760 34.228 298 134 8 17 306 28 271 1.26e-36 134
MS.gene005963.t1 MTR_4g058910 65.957 94 27 1 238 331 310 398 1.71e-35 133
MS.gene005963.t1 MTR_7g085410 45.455 165 82 4 143 306 95 252 4.24e-35 129
MS.gene005963.t1 MTR_4g071590 57.692 104 33 2 203 306 183 275 1.76e-34 128
MS.gene005963.t1 MTR_4g471590 57.692 104 33 2 203 306 183 275 1.76e-34 128
MS.gene005963.t1 MTR_6g032830 36.015 261 122 9 67 308 24 258 2.33e-34 127
MS.gene005963.t1 MTR_5g021340 35.766 274 121 10 48 308 135 366 8.22e-34 128
MS.gene005963.t1 MTR_3g117380 72.603 73 19 1 234 306 222 293 6.17e-33 124
MS.gene005963.t1 MTR_8g013950 81.538 65 12 0 242 306 226 290 9.46e-33 124
MS.gene005963.t1 MTR_5g007600 55.238 105 45 2 203 306 220 323 1.59e-31 121
MS.gene005963.t1 MTR_5g007600 54.000 100 44 2 207 305 122 220 2.07e-28 112
MS.gene005963.t1 MTR_8g076340 62.366 93 26 3 216 308 201 284 2.03e-31 119
MS.gene005963.t1 MTR_1g046680 80.000 65 13 0 242 306 172 236 2.12e-31 118
MS.gene005963.t1 MTR_7g446110 61.290 62 24 0 242 303 187 248 1.36e-20 89.7
MS.gene005963.t1 MTR_7g445950 58.065 62 26 0 242 303 120 181 2.56e-19 84.7
MS.gene005963.t1 MTR_7g445980 61.667 60 22 1 246 304 37 96 7.20e-19 80.9
MS.gene005963.t1 MTR_7g446070 36.567 134 65 2 175 303 75 193 8.44e-19 83.6
MS.gene005963.t1 MTR_7g106090 55.844 77 33 1 177 253 36 111 7.80e-17 75.5
MS.gene005963.t1 MTR_5g020230 52.083 48 23 0 227 274 107 154 2.98e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005963.t1 AT3G54810 71.875 96 20 2 240 328 227 322 2.45e-42 149
MS.gene005963.t1 AT3G54810 71.875 96 20 2 240 328 227 322 2.45e-42 149
MS.gene005963.t1 AT1G08010 40.254 236 111 5 85 318 89 296 3.21e-42 148
MS.gene005963.t1 AT1G08010 40.254 236 111 5 85 318 89 296 3.21e-42 148
MS.gene005963.t1 AT1G08010 40.254 236 111 5 85 318 89 296 3.21e-42 148
MS.gene005963.t1 AT5G25830 40.249 241 113 8 67 306 73 283 6.36e-38 138
MS.gene005963.t1 AT1G08000 42.051 195 88 3 115 309 116 285 2.63e-37 135
MS.gene005963.t1 AT1G08000 42.051 195 88 3 115 309 116 285 2.63e-37 135
MS.gene005963.t1 AT4G32890 34.641 306 120 12 17 306 20 261 7.11e-37 134
MS.gene005963.t1 AT5G66320 38.636 264 123 9 55 315 95 322 2.29e-36 134
MS.gene005963.t1 AT5G66320 38.636 264 123 9 55 315 95 322 2.29e-36 134
MS.gene005963.t1 AT3G51080 71.053 76 21 1 242 317 221 295 1.04e-31 121
MS.gene005963.t1 AT3G24050 78.462 65 14 0 242 306 194 258 1.08e-31 120
MS.gene005963.t1 AT4G36240 54.545 99 43 1 208 306 132 228 2.86e-31 118
MS.gene005963.t1 AT3G60530 74.242 66 17 0 241 306 157 222 9.68e-31 116
MS.gene005963.t1 AT2G28340 65.333 75 26 0 244 318 193 267 1.23e-30 117
MS.gene005963.t1 AT2G45050 72.727 66 18 0 241 306 178 243 3.02e-30 116
MS.gene005963.t1 AT4G34680 63.158 76 28 0 242 317 180 255 5.48e-29 112
MS.gene005963.t1 AT4G34680 63.158 76 28 0 242 317 180 255 5.48e-29 112
MS.gene005963.t1 AT3G45170 40.000 140 79 3 168 306 44 179 4.73e-28 108
MS.gene005963.t1 AT5G26930 54.545 44 18 1 234 277 20 61 4.71e-11 60.1

Find 105 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCAGCAGGCCGGTATTC+GGG 0.281895 1.3:-66098557 None:intergenic
AAATGAATCGTCCACAATAT+TGG 0.301923 1.3:-66097964 None:intergenic
ACAACTCATTGGCGAGTATC+TGG 0.323132 1.3:+66096048 MS.gene005963:CDS
CTGCTTTCAAGGACAGAAAT+AGG 0.331261 1.3:-66098083 None:intergenic
GTTCGAAGATCTTCTTTCAT+TGG 0.338071 1.3:+66097932 MS.gene005963:intron
GTCTCTCCAGAAGAGAGCTT+TGG 0.342561 1.3:-66098341 None:intergenic
CAATGCATGCATTTCCTAAC+AGG 0.350621 1.3:-66098437 None:intergenic
AAGGCGAGAACATTGGAGTT+TGG 0.360098 1.3:-66098677 None:intergenic
AGCAGGAACTGCTGCTTTCA+AGG 0.362822 1.3:-66098094 None:intergenic
ACTAGCAGCAGGCCGGTATT+CGG 0.363594 1.3:-66098558 None:intergenic
TCTCTACTCACCGGATCAAC+GGG 0.368770 1.3:-66096131 None:intergenic
GTCCGCAAGGCACGGGGATG+GGG 0.378251 1.3:-66098145 None:intergenic
GGCTGTGGGGCGTGGACGCT+TGG 0.384746 1.3:-66098177 None:intergenic
TATCCTTGCATCTCATTTCA+AGG 0.385918 1.3:-66098625 None:intergenic
CAATGGAGGGCAGGTCCAAT+GGG 0.390212 1.3:+66098479 MS.gene005963:CDS
CGTCCGCAAGGCACGGGGAT+GGG 0.396980 1.3:-66098146 None:intergenic
GAGGTCTGAAGTACTATTGC+CGG 0.397375 1.3:-66096096 None:intergenic
GCACTCCAATTCGCACAAAA+AGG 0.399415 1.3:+66098601 MS.gene005963:CDS
ACAATGGAGGGCAGGTCCAA+TGG 0.404651 1.3:+66098478 MS.gene005963:CDS
CCTTGGAATACAACATTTGA+AGG 0.412271 1.3:+66098696 MS.gene005963:CDS
TTGGTTTCTTGACAATAATC+CGG 0.416836 1.3:-66098322 None:intergenic
TGCCCTCCATTGTGGAGTCT+TGG 0.422561 1.3:-66098468 None:intergenic
CGTGCACGTCCGCAAGGCAC+GGG 0.424581 1.3:-66098152 None:intergenic
AAGAAACCAAAGCTCTCTTC+TGG 0.429167 1.3:+66098335 MS.gene005963:CDS
CCCTACTTCCTCTTCTGTTG+AGG 0.447338 1.3:+66098232 MS.gene005963:CDS
TTCTGTTCGCCACTGTCAGC+TGG 0.447903 1.3:-66098404 None:intergenic
AGAATGGTTCACTACCTGTT+AGG 0.449879 1.3:+66098423 MS.gene005963:CDS
GCTGTCAGCAAGGAGGACTC+TGG 0.452116 1.3:+66098035 MS.gene005963:CDS
AAACTCCAATGTTCTCGCCT+TGG 0.452478 1.3:+66098679 MS.gene005963:CDS
GAAAGCAGCAGTTCCTGCTC+TGG 0.453160 1.3:+66098098 MS.gene005963:CDS
TGCGAATTGGAGTGCACAGC+TGG 0.458877 1.3:-66098593 None:intergenic
ATCTGAAGACAGCGCCTTGG+TGG 0.463733 1.3:-66098279 None:intergenic
GGCGGAGCTCCCCGTTGATC+CGG 0.465097 1.3:+66096121 MS.gene005963:CDS
TGGTTTCTTGACAATAATCC+GGG 0.466726 1.3:-66098321 None:intergenic
GCGTGCACGTCCGCAAGGCA+CGG 0.472888 1.3:-66098153 None:intergenic
TGCGGTGTACGCTACAAGTC+GGG 0.473402 1.3:+66098524 MS.gene005963:CDS
GCGGTGTCAACGTCGTCGAC+GGG 0.476293 1.3:-66095993 None:intergenic
GGATTGAAGGCTGTGGGGCG+TGG 0.478430 1.3:-66098185 None:intergenic
CCTTCAAATGTTGTATTCCA+AGG 0.482354 1.3:-66098696 None:intergenic
AGCGGTGTCAACGTCGTCGA+CGG 0.482359 1.3:-66095994 None:intergenic
GCCGACTATTCCCCGAATAC+CGG 0.484840 1.3:+66098546 MS.gene005963:CDS
CGCGGCAAACTACAACTCAT+TGG 0.485302 1.3:+66096037 MS.gene005963:CDS
CTCTCTACTCACCGGATCAA+CGG 0.485631 1.3:-66096132 None:intergenic
AGATCTTCTTTCATTGGAGT+GGG 0.485791 1.3:+66097938 MS.gene005963:CDS
AAGATCTTCTTTCATTGGAG+TGG 0.486353 1.3:+66097937 MS.gene005963:CDS
ACGTCCGCAAGGCACGGGGA+TGG 0.486480 1.3:-66098147 None:intergenic
CCTCAACAGAAGAGGAAGTA+GGG 0.487341 1.3:-66098232 None:intergenic
GTTGTAGTTTGCCGCGGAGG+AGG 0.494768 1.3:-66096030 None:intergenic
CATGGCAGAACGAGGATTGA+AGG 0.499510 1.3:-66098198 None:intergenic
AGCAGCAGTTCCTGCTCTGG+TGG 0.510966 1.3:+66098101 MS.gene005963:CDS
GCCTTGGTGGAGATAACATT+AGG 0.511767 1.3:-66098266 None:intergenic
AGAACGAGGATTGAAGGCTG+TGG 0.514782 1.3:-66098192 None:intergenic
GAACGAGGATTGAAGGCTGT+GGG 0.520494 1.3:-66098191 None:intergenic
ATTGGACCTGCCCTCCATTG+TGG 0.524395 1.3:-66098476 None:intergenic
AACACCGAGTCAGACTCATT+TGG 0.524475 1.3:-66096071 None:intergenic
GGAGGGCAATGAAGCGGCAG+CGG 0.526729 1.3:-66096012 None:intergenic
GCCGGTATTCGGGGAATAGT+CGG 0.526797 1.3:-66098547 None:intergenic
CGCGGAGGAGGGCAATGAAG+CGG 0.533925 1.3:-66096018 None:intergenic
TCAAGGACAGAAATAGGACT+TGG 0.539012 1.3:-66098077 None:intergenic
CTTGCAGAGAAGAAAATGGC+TGG 0.540090 1.3:+66095915 None:intergenic
TTGCTGACAGCTGATGATGA+TGG 0.540546 1.3:-66098023 None:intergenic
CTGTCAGCAAGGAGGACTCT+GGG 0.542617 1.3:+66098036 MS.gene005963:CDS
AAAATCTGAAGACAGCGCCT+TGG 0.556439 1.3:-66098282 None:intergenic
TTGGTGCGTGCACGTCCGCA+AGG 0.556777 1.3:-66098158 None:intergenic
TAGCAGCAGGCCGGTATTCG+GGG 0.557503 1.3:-66098556 None:intergenic
CATGTTCTCCTCAACAGAAG+AGG 0.558411 1.3:-66098240 None:intergenic
GTCCCCATCCCCGTGCCTTG+CGG 0.560563 1.3:+66098143 MS.gene005963:CDS
TTGTAGTTTGCCGCGGAGGA+GGG 0.563526 1.3:-66096029 None:intergenic
TCAGCTGGAGCAGTAGGAAG+TGG 0.567950 1.3:-66098389 None:intergenic
CCACTGTCAGCTGGAGCAGT+AGG 0.567950 1.3:-66098395 None:intergenic
GGAGAGTCTCACAATGAAAG+TGG 0.570456 1.3:+66097992 MS.gene005963:CDS
TCCTCAACAGAAGAGGAAGT+AGG 0.578279 1.3:-66098233 None:intergenic
AACCAAGACTCCACAATGGA+GGG 0.579995 1.3:+66098466 MS.gene005963:CDS
AAGACTCCACAATGGAGGGC+AGG 0.580027 1.3:+66098470 MS.gene005963:CDS
GTATTCCAAGGCGAGAACAT+TGG 0.583263 1.3:-66098684 None:intergenic
CAAAACGTTGGACTAGCAGC+AGG 0.583276 1.3:-66098569 None:intergenic
CTGGCCAAATGAGTCTGACT+CGG 0.584259 1.3:+66096067 MS.gene005963:CDS
GCTGACAGTGGCGAACAGAA+TGG 0.590330 1.3:+66098407 MS.gene005963:CDS
TTGCGGTGTACGCTACAAGT+CGG 0.591752 1.3:+66098523 MS.gene005963:CDS
TGAGTTGTAGTTTGCCGCGG+AGG 0.592270 1.3:-66096033 None:intergenic
AAAACACTCTGCAATGCTTG+CGG 0.597816 1.3:+66098506 MS.gene005963:CDS
ATCATCATCAGCTGTCAGCA+AGG 0.601103 1.3:+66098025 MS.gene005963:CDS
CGCTTCATTGCCCTCCTCCG+CGG 0.601691 1.3:+66096019 MS.gene005963:CDS
GTGCACGTCCGCAAGGCACG+GGG 0.603513 1.3:-66098151 None:intergenic
TGCACAGCTGGACAAAACGT+TGG 0.609672 1.3:-66098581 None:intergenic
CAATGAGTTGTAGTTTGCCG+CGG 0.611265 1.3:-66096036 None:intergenic
GGTCCTTGAAATGAGATGCA+AGG 0.611789 1.3:+66098622 MS.gene005963:CDS
CAATAGTACTTCAGACCTCT+CGG 0.613630 1.3:+66096100 MS.gene005963:CDS
ACGTTGGACTAGCAGCAGGC+CGG 0.619617 1.3:-66098565 None:intergenic
CTCAACAGAAGAGGAAGTAG+GGG 0.627104 1.3:-66098231 None:intergenic
TAACCAAGACTCCACAATGG+AGG 0.629519 1.3:+66098465 MS.gene005963:CDS
CGGTGTCAACGTCGTCGACG+GGG 0.629631 1.3:-66095992 None:intergenic
AGTAGGGGAAATGAGTTGCA+TGG 0.640358 1.3:-66098216 None:intergenic
GCCTAATGTTATCTCCACCA+AGG 0.643801 1.3:+66098265 MS.gene005963:CDS
ATGAGTTGCATGGCAGAACG+AGG 0.661756 1.3:-66098206 None:intergenic
AACGAGGATTGAAGGCTGTG+GGG 0.665902 1.3:-66098190 None:intergenic
AATGGAGGGCAGGTCCAATG+GGG 0.678887 1.3:+66098480 MS.gene005963:CDS
CAACGGGGAGCTCCGCCGAG+AGG 0.679446 1.3:-66096115 None:intergenic
CCTACTGCTCCAGCTGACAG+TGG 0.685510 1.3:+66098395 MS.gene005963:CDS
ATCATCAGCTGTCAGCAAGG+AGG 0.691864 1.3:+66098028 MS.gene005963:CDS
GGAGTGGGTGTCCAATATTG+TGG 0.693703 1.3:+66097953 MS.gene005963:CDS
AGATAACCAAGACTCCACAA+TGG 0.734467 1.3:+66098462 MS.gene005963:CDS
CTCTACTCACCGGATCAACG+GGG 0.746525 1.3:-66096130 None:intergenic
GATGGGGACGTAAATCCCTG+TGG 0.752387 1.3:-66098129 None:intergenic
TAGTACTTCAGACCTCTCGG+CGG 0.759629 1.3:+66096103 MS.gene005963:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATAAAACAAACA+TGG - chr1.3:66097162-66097181 None:intergenic 10.0%
!! AAATAAAATAAAACAAACAT+GGG - chr1.3:66097161-66097180 None:intergenic 10.0%
!!! AACTTTACTTTATTTATTTT+TGG - chr1.3:66096791-66096810 None:intergenic 10.0%
!!! AATATAAAGTATTTATAGAA+AGG + chr1.3:66096920-66096939 MS.gene005963:intron 10.0%
!!! ATATAAAGTATTTATAGAAA+GGG + chr1.3:66096921-66096940 MS.gene005963:intron 10.0%
!! CTCTACAAAAAAAAATAATA+TGG - chr1.3:66096556-66096575 None:intergenic 15.0%
!! GAAAAAAATTATTTCGTAAT+AGG + chr1.3:66096220-66096239 MS.gene005963:intron 15.0%
!! TAAAAATTAACCTAAAAAAG+TGG - chr1.3:66096694-66096713 None:intergenic 15.0%
!!! AAGAGTAATATACTTTTTTT+TGG + chr1.3:66097233-66097252 MS.gene005963:intron 15.0%
!!! ACATGCATTTAATTTTATAT+TGG - chr1.3:66097462-66097481 None:intergenic 15.0%
!!! GATTTTTTTGCTTATATTTT+AGG - chr1.3:66097358-66097377 None:intergenic 15.0%
!!! TAATAAAGTTTTTTTTATCG+AGG + chr1.3:66096503-66096522 MS.gene005963:intron 15.0%
!!! TATAAGTTAGTAAAATCTTT+TGG - chr1.3:66096485-66096504 None:intergenic 15.0%
!!! TTTTTGCTTATAAAAAAAGA+CGG + chr1.3:66097637-66097656 MS.gene005963:intron 15.0%
!! ATTATTTGGATCATCATTAT+TGG + chr1.3:66097038-66097057 MS.gene005963:intron 20.0%
!! CACAATAATAACATAAAAGT+TGG - chr1.3:66097416-66097435 None:intergenic 20.0%
!! CATGATACTTATTAATACAA+AGG - chr1.3:66097725-66097744 None:intergenic 20.0%
!! GATGTAAAAATAATTCTCAT+TGG - chr1.3:66097529-66097548 None:intergenic 20.0%
!! TAAATAATGAACCTTCAAAT+TGG + chr1.3:66097113-66097132 MS.gene005963:intron 20.0%
!! TGAAGGTTCATTATTTATTT+AGG - chr1.3:66097110-66097129 None:intergenic 20.0%
!!! GTCGAAAAATGATTTTTTTA+AGG + chr1.3:66096603-66096622 MS.gene005963:intron 20.0%
!!! TAAAGGAATTTTCTACTTTT+TGG - chr1.3:66096844-66096863 None:intergenic 20.0%
!!! TTCTCATTGGTTAAAAATTA+TGG - chr1.3:66097516-66097535 None:intergenic 20.0%
!!! TTTTTGCTTATATTTTAGGA+CGG - chr1.3:66097354-66097373 None:intergenic 20.0%
! AATCACGATAAGTCAATAAA+AGG - chr1.3:66096397-66096416 None:intergenic 25.0%
! AGGTTCATTATTTATTTAGG+TGG - chr1.3:66097107-66097126 None:intergenic 25.0%
! ATCACGATAAGTCAATAAAA+GGG - chr1.3:66096396-66096415 None:intergenic 25.0%
! ATTAAAATGTCATTCACATG+TGG - chr1.3:66097300-66097319 None:intergenic 25.0%
! CAAGAAGAAAAAGAAAATCA+AGG + chr1.3:66098364-66098383 MS.gene005963:CDS 25.0%
! GAAAATTCCTTTACTTGAAT+TGG + chr1.3:66096851-66096870 MS.gene005963:intron 25.0%
! GGTTCATTATTTATTTAGGT+GGG - chr1.3:66097106-66097125 None:intergenic 25.0%
! TCTTCATCTATTATTCTTAG+AGG - chr1.3:66097847-66097866 None:intergenic 25.0%
!! CTTGATTTTCTTTTTCTTCT+TGG - chr1.3:66098366-66098385 None:intergenic 25.0%
!!! AATCATTTTTCGACATGAAT+TGG - chr1.3:66096597-66096616 None:intergenic 25.0%
!!! ATAATTTTTTGTGTTCTCCT+TGG - chr1.3:66097569-66097588 None:intergenic 25.0%
AAACAAAACATATGTGTGCA+TGG - chr1.3:66097698-66097717 None:intergenic 30.0%
AAATGAATCGTCCACAATAT+TGG - chr1.3:66097967-66097986 None:intergenic 30.0%
AACCAATACTTAGTGATTTG+AGG - chr1.3:66097593-66097612 None:intergenic 30.0%
AAGAATAAGAACTTGTGCTT+TGG - chr1.3:66097202-66097221 None:intergenic 30.0%
ATAATGAACCTTCAAATTGG+TGG + chr1.3:66097116-66097135 MS.gene005963:intron 30.0%
ATGACTTCCAATTCAAGTAA+AGG - chr1.3:66096861-66096880 None:intergenic 30.0%
TACCTCAAATCACTAAGTAT+TGG + chr1.3:66097588-66097607 MS.gene005963:intron 30.0%
TATTTATAGAAAGGGCAGTT+TGG + chr1.3:66096929-66096948 MS.gene005963:intron 30.0%
TGATCAATAAATCGCATCAT+TGG - chr1.3:66097762-66097781 None:intergenic 30.0%
TGCTTATAAAAAAAGACGGA+GGG + chr1.3:66097641-66097660 MS.gene005963:intron 30.0%
TTGCTTATAAAAAAAGACGG+AGG + chr1.3:66097640-66097659 MS.gene005963:intron 30.0%
TTGGTTTCTTGACAATAATC+CGG - chr1.3:66098325-66098344 None:intergenic 30.0%
TTTCCATGAAACTTATTCCA+AGG + chr1.3:66097549-66097568 MS.gene005963:intron 30.0%
!!! ATTCTTAGAGGCTAGTTTTA+TGG - chr1.3:66097835-66097854 None:intergenic 30.0%
!!! TTATTTTTGGTCAAGTAGAG+TGG - chr1.3:66096778-66096797 None:intergenic 30.0%
!!! TTGCATATTGCCACTTTTTT+AGG + chr1.3:66096681-66096700 MS.gene005963:intron 30.0%
AAACCATTCATGATGTTGCT+AGG - chr1.3:66096426-66096445 None:intergenic 35.0%
AAAGCTACAAACAGACAATC+AGG - chr1.3:66096303-66096322 None:intergenic 35.0%
AACCATTCATGATGTTGCTA+GGG - chr1.3:66096425-66096444 None:intergenic 35.0%
AAGATCTTCTTTCATTGGAG+TGG + chr1.3:66097937-66097956 MS.gene005963:CDS 35.0%
AGATCTTCTTTCATTGGAGT+GGG + chr1.3:66097938-66097957 MS.gene005963:CDS 35.0%
AGTGGCAATATGCAATTGAT+TGG - chr1.3:66096676-66096695 None:intergenic 35.0%
CATCATAATAGTTCCGTGTA+CGG + chr1.3:66096276-66096295 MS.gene005963:intron 35.0%
GTCATAATCAAGCAATCAGA+CGG - chr1.3:66096257-66096276 None:intergenic 35.0%
GTTCGAAGATCTTCTTTCAT+TGG + chr1.3:66097932-66097951 MS.gene005963:intron 35.0%
TAGATTGACCACCAATTTGA+AGG - chr1.3:66097127-66097146 None:intergenic 35.0%
TATCCTTGCATCTCATTTCA+AGG - chr1.3:66098628-66098647 None:intergenic 35.0%
TCTCCTTGGAATAAGTTTCA+TGG - chr1.3:66097555-66097574 None:intergenic 35.0%
TGGTTTCTTGACAATAATCC+GGG - chr1.3:66098324-66098343 None:intergenic 35.0%
TTAAGTAACTCTCTACTCAC+CGG - chr1.3:66096143-66096162 None:intergenic 35.0%
TTCAGGAAACTTGATTGTGT+CGG + chr1.3:66096958-66096977 MS.gene005963:intron 35.0%
! AAACAAACATGGGTTGTTAC+TGG - chr1.3:66097151-66097170 None:intergenic 35.0%
! TTGAAAATTTTGCTGAGTCC+CGG + chr1.3:66098303-66098322 MS.gene005963:CDS 35.0%
!!! TGCTTATATTTTAGGACGGA+GGG - chr1.3:66097350-66097369 None:intergenic 35.0%
!!! TTGCTTATATTTTAGGACGG+AGG - chr1.3:66097351-66097370 None:intergenic 35.0%
AAAACACTCTGCAATGCTTG+CGG + chr1.3:66098506-66098525 MS.gene005963:CDS 40.0%
AAGAAACCAAAGCTCTCTTC+TGG + chr1.3:66098335-66098354 MS.gene005963:CDS 40.0%
AGAATGGTTCACTACCTGTT+AGG + chr1.3:66098423-66098442 MS.gene005963:CDS 40.0%
AGATAACCAAGACTCCACAA+TGG + chr1.3:66098462-66098481 MS.gene005963:CDS 40.0%
CAATAGTACTTCAGACCTCT+CGG + chr1.3:66096100-66096119 MS.gene005963:CDS 40.0%
CAATGCATGCATTTCCTAAC+AGG - chr1.3:66098440-66098459 None:intergenic 40.0%
CTGCTTTCAAGGACAGAAAT+AGG - chr1.3:66098086-66098105 None:intergenic 40.0%
TCAAGGACAGAAATAGGACT+TGG - chr1.3:66098080-66098099 None:intergenic 40.0%
TTCCCTAGCAACATCATGAA+TGG + chr1.3:66096420-66096439 MS.gene005963:intron 40.0%
! AAGGACCTTTTTGTGCGAAT+TGG - chr1.3:66098609-66098628 None:intergenic 40.0%
! TGTGGACGATTCATTTTGTG+AGG + chr1.3:66097971-66097990 MS.gene005963:CDS 40.0%
!! CAAGCATTGCAGAGTGTTTT+TGG - chr1.3:66098506-66098525 None:intergenic 40.0%
AAAATCTGAAGACAGCGCCT+TGG - chr1.3:66098285-66098304 None:intergenic 45.0%
AAACTCCAATGTTCTCGCCT+TGG + chr1.3:66098679-66098698 MS.gene005963:CDS 45.0%
AACACCGAGTCAGACTCATT+TGG - chr1.3:66096074-66096093 None:intergenic 45.0%
AACCAAGACTCCACAATGGA+GGG + chr1.3:66098466-66098485 MS.gene005963:CDS 45.0%
AACCACATAATCCGTGTGAC+AGG - chr1.3:66096337-66096356 None:intergenic 45.0%
AAGGCGAGAACATTGGAGTT+TGG - chr1.3:66098680-66098699 None:intergenic 45.0%
ACAACTCATTGGCGAGTATC+TGG + chr1.3:66096048-66096067 MS.gene005963:CDS 45.0%
ACCCCTCAAATTGGAGAGTA+GGG - chr1.3:66097007-66097026 None:intergenic 45.0%
ACCCTACTCTCCAATTTGAG+GGG + chr1.3:66097003-66097022 MS.gene005963:intron 45.0%
ACTATTTCCAGCCTGTCACA+CGG + chr1.3:66096323-66096342 MS.gene005963:intron 45.0%
AGTAGGGGAAATGAGTTGCA+TGG - chr1.3:66098219-66098238 None:intergenic 45.0%
ATCATCATCAGCTGTCAGCA+AGG + chr1.3:66098025-66098044 MS.gene005963:CDS 45.0%
CACCCTACTCTCCAATTTGA+GGG + chr1.3:66097002-66097021 MS.gene005963:intron 45.0%
CATGTTCTCCTCAACAGAAG+AGG - chr1.3:66098243-66098262 None:intergenic 45.0%
CCTCAACAGAAGAGGAAGTA+GGG - chr1.3:66098235-66098254 None:intergenic 45.0%
CTCAACAGAAGAGGAAGTAG+GGG - chr1.3:66098234-66098253 None:intergenic 45.0%
GCACTCCAATTCGCACAAAA+AGG + chr1.3:66098601-66098620 MS.gene005963:CDS 45.0%
GCCTAATGTTATCTCCACCA+AGG + chr1.3:66098265-66098284 MS.gene005963:CDS 45.0%
GCCTTGGTGGAGATAACATT+AGG - chr1.3:66098269-66098288 None:intergenic 45.0%
GGAGAGTCTCACAATGAAAG+TGG + chr1.3:66097992-66098011 MS.gene005963:CDS 45.0%
GGTCCTTGAAATGAGATGCA+AGG + chr1.3:66098622-66098641 MS.gene005963:CDS 45.0%
GGTGCGCTGTCTCAATTATT+TGG + chr1.3:66097024-66097043 MS.gene005963:intron 45.0%
GTATTCCAAGGCGAGAACAT+TGG - chr1.3:66098687-66098706 None:intergenic 45.0%
TAACCAAGACTCCACAATGG+AGG + chr1.3:66098465-66098484 MS.gene005963:CDS 45.0%
TCCTCAACAGAAGAGGAAGT+AGG - chr1.3:66098236-66098255 None:intergenic 45.0%
! CAATGAGTTGTAGTTTGCCG+CGG - chr1.3:66096039-66096058 None:intergenic 45.0%
! GAGGTCTGAAGTACTATTGC+CGG - chr1.3:66096099-66096118 None:intergenic 45.0%
! TTGATTGTGTCGGTGACATG+TGG + chr1.3:66096968-66096987 MS.gene005963:intron 45.0%
! TTGCTGACAGCTGATGATGA+TGG - chr1.3:66098026-66098045 None:intergenic 45.0%
!!! GGGCAGTTTGGTCATTTTTC+AGG + chr1.3:66096941-66096960 MS.gene005963:intron 45.0%
!!! TTATTTTTATTTTCTAAAAT+TGG + chr1.3:66096817-66096836 MS.gene005963:intron 5.0%
AACGAGGATTGAAGGCTGTG+GGG - chr1.3:66098193-66098212 None:intergenic 50.0%
ACATAATCCGTGTGACAGGC+TGG - chr1.3:66096333-66096352 None:intergenic 50.0%
AGAACGAGGATTGAAGGCTG+TGG - chr1.3:66098195-66098214 None:intergenic 50.0%
ATCATCAGCTGTCAGCAAGG+AGG + chr1.3:66098028-66098047 MS.gene005963:CDS 50.0%
ATGAGTTGCATGGCAGAACG+AGG - chr1.3:66098209-66098228 None:intergenic 50.0%
CACCCCTCAAATTGGAGAGT+AGG - chr1.3:66097008-66097027 None:intergenic 50.0%
CAGACAATCAGGTCCGTACA+CGG - chr1.3:66096292-66096311 None:intergenic 50.0%
CATGGCAGAACGAGGATTGA+AGG - chr1.3:66098201-66098220 None:intergenic 50.0%
CCACCCTACTCTCCAATTTG+AGG + chr1.3:66097001-66097020 MS.gene005963:intron 50.0%
CCCTACTTCCTCTTCTGTTG+AGG + chr1.3:66098232-66098251 MS.gene005963:CDS 50.0%
CCTCAAATTGGAGAGTAGGG+TGG - chr1.3:66097004-66097023 None:intergenic 50.0%
CGCGGCAAACTACAACTCAT+TGG + chr1.3:66096037-66096056 MS.gene005963:CDS 50.0%
CTCTCTACTCACCGGATCAA+CGG - chr1.3:66096135-66096154 None:intergenic 50.0%
CTGGCCAAATGAGTCTGACT+CGG + chr1.3:66096067-66096086 MS.gene005963:CDS 50.0%
GAACGAGGATTGAAGGCTGT+GGG - chr1.3:66098194-66098213 None:intergenic 50.0%
GCTCTGGTGGAAAAACCACA+GGG + chr1.3:66098114-66098133 MS.gene005963:CDS 50.0%
GGAGTGGGTGTCCAATATTG+TGG + chr1.3:66097953-66097972 MS.gene005963:CDS 50.0%
GTCTCTCCAGAAGAGAGCTT+TGG - chr1.3:66098344-66098363 None:intergenic 50.0%
TAGTACTTCAGACCTCTCGG+CGG + chr1.3:66096103-66096122 MS.gene005963:CDS 50.0%
TCTCTACTCACCGGATCAAC+GGG - chr1.3:66096134-66096153 None:intergenic 50.0%
TGCACAGCTGGACAAAACGT+TGG - chr1.3:66098584-66098603 None:intergenic 50.0%
TGCTCTGGTGGAAAAACCAC+AGG + chr1.3:66098113-66098132 MS.gene005963:CDS 50.0%
TTGCGGTGTACGCTACAAGT+CGG + chr1.3:66098523-66098542 MS.gene005963:CDS 50.0%
! AGCCTGTCACACGGATTATG+TGG + chr1.3:66096332-66096351 MS.gene005963:intron 50.0%
! TGTGGTTTTTCCACCAGAGC+AGG - chr1.3:66098114-66098133 None:intergenic 50.0%
!! AGCAGGAACTGCTGCTTTCA+AGG - chr1.3:66098097-66098116 None:intergenic 50.0%
!! CAAAACGTTGGACTAGCAGC+AGG - chr1.3:66098572-66098591 None:intergenic 50.0%
!!! CAGAGTGTTTTTGGCCCCAT+TGG - chr1.3:66098497-66098516 None:intergenic 50.0%
!!! GAGTCTGACTCGGTGTTTTC+CGG + chr1.3:66096077-66096096 MS.gene005963:CDS 50.0%
AAGACTCCACAATGGAGGGC+AGG + chr1.3:66098470-66098489 MS.gene005963:CDS 55.0%
AATGGAGGGCAGGTCCAATG+GGG + chr1.3:66098480-66098499 MS.gene005963:CDS 55.0%
ACAATGGAGGGCAGGTCCAA+TGG + chr1.3:66098478-66098497 MS.gene005963:CDS 55.0%
ACTAGCAGCAGGCCGGTATT+CGG - chr1.3:66098561-66098580 None:intergenic 55.0%
AGACAGCGCACCCCTCAAAT+TGG - chr1.3:66097016-66097035 None:intergenic 55.0%
ATCTGAAGACAGCGCCTTGG+TGG - chr1.3:66098282-66098301 None:intergenic 55.0%
ATTGGACCTGCCCTCCATTG+TGG - chr1.3:66098479-66098498 None:intergenic 55.0%
CAATGGAGGGCAGGTCCAAT+GGG + chr1.3:66098479-66098498 MS.gene005963:CDS 55.0%
CTCTACTCACCGGATCAACG+GGG - chr1.3:66096133-66096152 None:intergenic 55.0%
CTGTCAGCAAGGAGGACTCT+GGG + chr1.3:66098036-66098055 MS.gene005963:CDS 55.0%
GAAAGCAGCAGTTCCTGCTC+TGG + chr1.3:66098098-66098117 MS.gene005963:CDS 55.0%
GATGGGGACGTAAATCCCTG+TGG - chr1.3:66098132-66098151 None:intergenic 55.0%
GCCGACTATTCCCCGAATAC+CGG + chr1.3:66098546-66098565 MS.gene005963:CDS 55.0%
GCCGGTATTCGGGGAATAGT+CGG - chr1.3:66098550-66098569 None:intergenic 55.0%
GCTGACAGTGGCGAACAGAA+TGG + chr1.3:66098407-66098426 MS.gene005963:CDS 55.0%
TCAGCTGGAGCAGTAGGAAG+TGG - chr1.3:66098392-66098411 None:intergenic 55.0%
TGCCCTCCATTGTGGAGTCT+TGG - chr1.3:66098471-66098490 None:intergenic 55.0%
TGCGAATTGGAGTGCACAGC+TGG - chr1.3:66098596-66098615 None:intergenic 55.0%
TGCGGTGTACGCTACAAGTC+GGG + chr1.3:66098524-66098543 MS.gene005963:CDS 55.0%
TTCTGTTCGCCACTGTCAGC+TGG - chr1.3:66098407-66098426 None:intergenic 55.0%
! TGAGTTGTAGTTTGCCGCGG+AGG - chr1.3:66096036-66096055 None:intergenic 55.0%
! TTGTAGTTTGCCGCGGAGGA+GGG - chr1.3:66096032-66096051 None:intergenic 55.0%
AGCAGCAGTTCCTGCTCTGG+TGG + chr1.3:66098101-66098120 MS.gene005963:CDS 60.0%
CCACTGTCAGCTGGAGCAGT+AGG - chr1.3:66098398-66098417 None:intergenic 60.0%
CCTACTGCTCCAGCTGACAG+TGG + chr1.3:66098395-66098414 MS.gene005963:CDS 60.0%
CTAGCAGCAGGCCGGTATTC+GGG - chr1.3:66098560-66098579 None:intergenic 60.0%
GCTGTCAGCAAGGAGGACTC+TGG + chr1.3:66098035-66098054 MS.gene005963:CDS 60.0%
TAGCAGCAGGCCGGTATTCG+GGG - chr1.3:66098559-66098578 None:intergenic 60.0%
! AGCGGTGTCAACGTCGTCGA+CGG - chr1.3:66095997-66096016 None:intergenic 60.0%
! GTTGTAGTTTGCCGCGGAGG+AGG - chr1.3:66096033-66096052 None:intergenic 60.0%
!! ACGTTGGACTAGCAGCAGGC+CGG - chr1.3:66098568-66098587 None:intergenic 60.0%
CGCGGAGGAGGGCAATGAAG+CGG - chr1.3:66096021-66096040 None:intergenic 65.0%
CGCTTCATTGCCCTCCTCCG+CGG + chr1.3:66096019-66096038 MS.gene005963:CDS 65.0%
GGAGGGCAATGAAGCGGCAG+CGG - chr1.3:66096015-66096034 None:intergenic 65.0%
GGATTGAAGGCTGTGGGGCG+TGG - chr1.3:66098188-66098207 None:intergenic 65.0%
TTGGTGCGTGCACGTCCGCA+AGG - chr1.3:66098161-66098180 None:intergenic 65.0%
! CGGTGTCAACGTCGTCGACG+GGG - chr1.3:66095995-66096014 None:intergenic 65.0%
! GCGGTGTCAACGTCGTCGAC+GGG - chr1.3:66095996-66096015 None:intergenic 65.0%
ACGTCCGCAAGGCACGGGGA+TGG - chr1.3:66098150-66098169 None:intergenic 70.0%
CGTCCGCAAGGCACGGGGAT+GGG - chr1.3:66098149-66098168 None:intergenic 70.0%
CGTGCACGTCCGCAAGGCAC+GGG - chr1.3:66098155-66098174 None:intergenic 70.0%
GCGTGCACGTCCGCAAGGCA+CGG - chr1.3:66098156-66098175 None:intergenic 70.0%
GGCGGAGCTCCCCGTTGATC+CGG + chr1.3:66096121-66096140 MS.gene005963:CDS 70.0%
GTCCCCATCCCCGTGCCTTG+CGG + chr1.3:66098143-66098162 MS.gene005963:CDS 70.0%
GTCCGCAAGGCACGGGGATG+GGG - chr1.3:66098148-66098167 None:intergenic 70.0%
GTGCACGTCCGCAAGGCACG+GGG - chr1.3:66098154-66098173 None:intergenic 70.0%
CAACGGGGAGCTCCGCCGAG+AGG - chr1.3:66096118-66096137 None:intergenic 75.0%
GGCTGTGGGGCGTGGACGCT+TGG - chr1.3:66098180-66098199 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 66095930 66098715 66095930 ID=MS.gene005963
chr1.3 mRNA 66095930 66098715 66095930 ID=MS.gene005963.t1;Parent=MS.gene005963
chr1.3 exon 66095930 66096142 66095930 ID=MS.gene005963.t1.exon1;Parent=MS.gene005963.t1
chr1.3 CDS 66095930 66096142 66095930 ID=cds.MS.gene005963.t1;Parent=MS.gene005963.t1
chr1.3 exon 66097933 66098715 66097933 ID=MS.gene005963.t1.exon2;Parent=MS.gene005963.t1
chr1.3 CDS 66097933 66098715 66097933 ID=cds.MS.gene005963.t1;Parent=MS.gene005963.t1
Gene Sequence

>MS.gene005963

ATGGCTGGAACAATGAACGAGATTGATTGCTTCAACTTATTTGACAACATTGATGACATCTACCCCGTCGACGACGTTGACACCGCTGCCGCTTCATTGCCCTCCTCCGCGGCAAACTACAACTCATTGGCGAGTATCTGGCCAAATGAGTCTGACTCGGTGTTTTCCGGCAATAGTACTTCAGACCTCTCGGCGGAGCTCCCCGTTGATCCGGTGAGTAGAGAGTTACTTAAAGTATAATTTATTTTATTTTATTTTGTTGTTATTATTTCATAATAACAGCTTTGTCAGAAAAAAATTATTTCGTAATAGGTTTGTTTGTAACCGTCTGATTGCTTGATTATGACATCATAATAGTTCCGTGTACGGACCTGATTGTCTGTTTGTAGCTTTACTATTTCCAGCCTGTCACACGGATTATGTGGTTTTTCTAATTTTTTATTGACTCATTTTGATTTATTTTCCCTTTTATTGACTTATCGTGATTTTTTTCCCTAGCAACATCATGAATGGTTTACAGTTCAAAATTTAAAAAAAGAAAAGAAAAACAGTCCAAAAGATTTTACTAACTTATAATAAAGTTTTTTTTATCGAGGAAATTCATTTTCATGTTTTTTTCTTTGCCATATTATTTTTTTTTGTAGAGAATTTCAAAATTTTAAATCCAATTCATGTCGAAAAATGATTTTTTTAAGGTTTAATTTATATACATTTGACAATTTAAATTATTTGCACGTTTGAATCCAATCAATTGCATATTGCCACTTTTTTAGGTTAATTTTTAAAATATCTTCTTAAATATCTATTTAATATAATTTACTTATATACACTTATAAAAAATCAAACCACTCTACTTGACCAAAAATAAATAAAGTAAAGTTAATATTTTATTTTTATTTTCTAAAATTGGTCCAAAAAGTAGAAAATTCCTTTACTTGAATTGGAAGTCATTATTATTAACTAATTGAGAACTTTTATAATATACTACTAAATATAAAGTATTTATAGAAAGGGCAGTTTGGTCATTTTTCAGGAAACTTGATTGTGTCGGTGACATGTGGCACATCACTTCCACCCTACTCTCCAATTTGAGGGGTGCGCTGTCTCAATTATTTGGATCATCATTATTGGATGTCATGTGCTTTTGCATGTGATTATCACGTGATGTGAGCACCCACCTAAATAAATAATGAACCTTCAAATTGGTGGTCAATCTAGCCAGTAACAACCCATGTTTGTTTTATTTTATTTTATTTTATTTAATTCATACCAAAGCACAAGTTCTTATTCTTCATTGAATTTGAAGAGTAATATACTTTTTTTTGGTTGTATATGCTCTACTAAACTGCATTCTTTGCTTGAACTGTCCACATGTGAATGACATTTTAATAAATGATTGTGTTTATACTTCAATACTCCCTCCGTCCTAAAATATAAGCAAAAAAATCTCATTTTCTTTGTATCAAAATATAAGCAAAAAACACCAACTTTTATGTTATTATTGTGTTTTTTCAAACAAATCATCTTTTCCAATATAAAATTAAATGCATGTTGCATTCAATTTGTTTCTCTTTTTATTTTCTCCATAATTTTTAACCAATGAGAATTATTTTTACATCTTTCCATGAAACTTATTCCAAGGAGAACACAAAAAATTATACCTCAAATCACTAAGTATTGGTTTTCTTAATAAGTGTGAATTAGTGTTTTTTGCTTATAAAAAAAGACGGAGGGAGTATTAGTTATATGTAAAAACATGCTTGCACCATGCACACATATGTTTTGTTTTTCACCTTTGTATTAATAAGTATCATGATATCTCATTTGATCCAATGATGCGATTTATTGATCAAATTCTGAAAAATAACTTGTACATGATGCAAAACTTATCTCATTTGTGCACCATAAAACTAGCCTCTAAGAATAATAGATGAAGATGTGAATTTAAGTTTTGTTCTTTTAATAAAATTGTGATTTTGATCAATCTTGTTATATTATTGCAGTTCGAAGATCTTCTTTCATTGGAGTGGGTGTCCAATATTGTGGACGATTCATTTTGTGAGGAGAGTCTCACAATGAAAGTGGAGCAGCAGCCATCATCATCAGCTGTCAGCAAGGAGGACTCTGGGCATTATCAATTTCAGACACCAAGTCCTATTTCTGTCCTTGAAAGCAGCAGTTCCTGCTCTGGTGGAAAAACCACAGGGATTTACGTCCCCATCCCCGTGCCTTGCGGACGTGCACGCACCAAGCGTCCACGCCCCACAGCCTTCAATCCTCGTTCTGCCATGCAACTCATTTCCCCTACTTCCTCTTCTGTTGAGGAGAACATGCAGCCTAATGTTATCTCCACCAAGGCGCTGTCTTCAGATTTTGAAAATTTTGCTGAGTCCCGGATTATTGTCAAGAAACCAAAGCTCTCTTCTGGAGAGACCAAGAAGAAAAAGAAAATCAAGGCACCACTTCCTACTGCTCCAGCTGACAGTGGCGAACAGAATGGTTCACTACCTGTTAGGAAATGCATGCATTGTGAGATAACCAAGACTCCACAATGGAGGGCAGGTCCAATGGGGCCAAAAACACTCTGCAATGCTTGCGGTGTACGCTACAAGTCGGGCCGACTATTCCCCGAATACCGGCCTGCTGCTAGTCCAACGTTTTGTCCAGCTGTGCACTCCAATTCGCACAAAAAGGTCCTTGAAATGAGATGCAAGGATAGTTTTGAATCAGATTCAGCTGAACTCATTCCAAACTCCAATGTTCTCGCCTTGGAATACAACATTTGA
Protein sequence

>MS.gene005963.t1

MAGTMNEIDCFNLFDNIDDIYPVDDVDTAAASLPSSAANYNSLASIWPNESDSVFSGNSTSDLSAELPVDPFEDLLSLEWVSNIVDDSFCEESLTMKVEQQPSSSAVSKEDSGHYQFQTPSPISVLESSSSCSGGKTTGIYVPIPVPCGRARTKRPRPTAFNPRSAMQLISPTSSSVEENMQPNVISTKALSSDFENFAESRIIVKKPKLSSGETKKKKKIKAPLPTAPADSGEQNGSLPVRKCMHCEITKTPQWRAGPMGPKTLCNACGVRYKSGRLFPEYRPAASPTFCPAVHSNSHKKVLEMRCKDSFESDSAELIPNSNVLALEYNI