AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00616

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00616.t1 MTR_2g006150 91.555 521 10 9 1 521 1 487 0.0 922
MS.gene00616.t1 MTR_2g006150 93.978 465 7 8 1 465 1 444 0.0 851
MS.gene00616.t1 MTR_5g039600 92.045 88 6 1 131 217 61 148 2.48e-49 175
MS.gene00616.t1 MTR_4g108370 65.909 132 34 3 76 205 44 166 2.01e-45 165
MS.gene00616.t1 MTR_1g114380 80.488 82 13 1 127 208 27 105 6.33e-38 140
MS.gene00616.t1 MTR_7g028160 53.378 148 59 3 112 255 25 166 8.60e-38 140
MS.gene00616.t1 MTR_1g101810 59.292 113 42 1 112 224 44 152 4.23e-36 137
MS.gene00616.t1 MTR_1g101810 59.292 113 42 1 112 224 74 182 1.30e-35 137
MS.gene00616.t1 MTR_1g038650 75.000 84 18 1 121 204 20 100 1.44e-35 133
MS.gene00616.t1 MTR_8g033070 78.947 76 16 0 133 208 77 152 2.46e-35 135
MS.gene00616.t1 MTR_1g063870 80.282 71 14 0 128 198 41 111 1.04e-34 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00616.t1 AT1G58100 46.604 427 129 17 76 478 13 364 2.20e-80 256
MS.gene00616.t1 AT1G35560 42.899 338 94 10 133 464 52 296 2.99e-59 199
MS.gene00616.t1 AT1G58100 41.452 427 127 19 76 478 13 340 1.04e-56 194
MS.gene00616.t1 AT1G72010 40.604 298 104 10 125 399 52 299 4.56e-48 171
MS.gene00616.t1 AT1G69690 58.599 157 38 3 131 275 46 187 1.34e-41 152
MS.gene00616.t1 AT5G23280 77.108 83 16 1 126 208 32 111 5.09e-37 137
MS.gene00616.t1 AT2G45680 62.500 112 40 1 104 213 41 152 1.82e-36 139
MS.gene00616.t1 AT5G08330 68.750 96 27 1 111 206 9 101 9.79e-36 134
MS.gene00616.t1 AT3G27010 51.493 134 48 2 64 197 23 139 1.02e-34 133
MS.gene00616.t1 AT3G27010 51.493 134 48 2 64 197 23 139 1.02e-34 133
MS.gene00616.t1 AT5G51910 78.205 78 16 1 132 208 52 129 8.66e-34 130
MS.gene00616.t1 AT5G51910 78.205 78 16 1 132 208 52 129 8.66e-34 130
MS.gene00616.t1 AT2G37000 80.000 60 12 0 136 195 43 102 3.29e-27 108
MS.gene00616.t1 AT5G41030 51.546 97 35 1 133 229 65 149 1.50e-26 108
MS.gene00616.t1 AT3G45150 61.905 63 24 0 133 195 14 76 1.29e-21 92.0

Find 148 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGCCATCACCTTTCATTA+TGG 0.235639 2.1:+75803838 MS.gene00616:CDS
CTTGTTGTTGGTGGTGGGGA+TGG 0.241546 2.1:-75803760 None:intergenic
AGGTTCAATGTTCCAGCTTC+TGG 0.242129 2.1:+75804964 MS.gene00616:CDS
TTGAAGAAAATGGTTGAATT+TGG 0.273936 2.1:-75804039 None:intergenic
TCACCAACAACAGGATGATA+TGG 0.275830 2.1:+75804648 MS.gene00616:CDS
TTGTTGTTGGTGGTGGGGAT+GGG 0.292871 2.1:-75803759 None:intergenic
TTCCAGTTGACCAGAGAATT+AGG 0.308053 2.1:+75804181 MS.gene00616:CDS
GATAGTCATTGTGAGGTATT+AGG 0.316778 2.1:-75805249 None:intergenic
GACGGTGGTGGAGGAGGTGG+TGG 0.325996 2.1:+75804679 MS.gene00616:CDS
GACTGAACATGAGGTGTAAA+AGG 0.331793 2.1:-75804886 None:intergenic
ATGATTCTTGTTGTTGGTGG+TGG 0.333014 2.1:-75803766 None:intergenic
CGGGGAGTGGAAGTACAATT+TGG 0.342926 2.1:+75804791 MS.gene00616:CDS
TCTTCTCCACTCTCATCATT+AGG 0.347346 2.1:-75805222 None:intergenic
TGTTGTTGGTGGTGGGGATG+GGG 0.348885 2.1:-75803758 None:intergenic
CGCCCATATCATCCTGTTGT+TGG 0.355044 2.1:-75804651 None:intergenic
AGGCCATCACCTTTCATTAT+GGG 0.359459 2.1:+75803839 MS.gene00616:CDS
GTGGGGATGGGGATGAATAT+TGG 0.363141 2.1:-75803747 None:intergenic
GAAGGTGACGGTGGTGGAGG+AGG 0.367290 2.1:+75804673 MS.gene00616:CDS
GGCTGGGATTGTTCCGGTGC+CGG 0.367521 2.1:-75804272 None:intergenic
CTGTCCTTGGTGGATCGTTT+TGG 0.369093 2.1:-75804094 None:intergenic
TACCAACACGCTCCTCTGTT+AGG 0.370083 2.1:+75804424 MS.gene00616:CDS
GTTGTTGCTGTTGCTGTTGT+TGG 0.372670 2.1:-75804468 None:intergenic
TGGACGTGGTGGTTTACAAT+TGG 0.382459 2.1:+75805017 MS.gene00616:CDS
TGATTCTTGTTGTTGGTGGT+GGG 0.388715 2.1:-75803765 None:intergenic
GTATCAACCATCACAGCATT+TGG 0.390115 2.1:+75805047 MS.gene00616:CDS
GCGGTTGAAGTTGAAGAAAA+TGG 0.393103 2.1:-75804049 None:intergenic
GGCCTAATTCTCTGGTCAAC+TGG 0.396838 2.1:-75804183 None:intergenic
GTAGCCCACATGGCTGTTGC+CGG 0.398412 2.1:-75804766 None:intergenic
GGATTTGTGGCCTAATTCTC+TGG 0.401966 2.1:-75804191 None:intergenic
GATTCTTGTTGTTGGTGGTG+GGG 0.404293 2.1:-75803764 None:intergenic
GGTGGTGGAGGAGGTGGTGG+TGG 0.404546 2.1:+75804682 MS.gene00616:CDS
GTGAGGATGATTCTTGTTGT+TGG 0.411900 2.1:-75803772 None:intergenic
TTGTTTCACCATCGGATTTG+TGG 0.412133 2.1:-75804204 None:intergenic
CCACTCTCATCATTAGGTTG+AGG 0.418843 2.1:-75805216 None:intergenic
GGTGACGGTGGTGGAGGAGG+TGG 0.419720 2.1:+75804676 MS.gene00616:CDS
TATCAACCATCACAGCATTT+GGG 0.420346 2.1:+75805048 MS.gene00616:CDS
GGACGTGGTGGTTTACAATT+GGG 0.421245 2.1:+75805018 MS.gene00616:CDS
TCAACAACATTCAATGTCAT+TGG 0.422221 2.1:+75805173 MS.gene00616:CDS
TTGTTGTTGTTGGATACATC+TGG 0.423257 2.1:-75804010 None:intergenic
GACGTTGAGCGTTGAGAAAT+TGG 0.424642 2.1:-75804293 None:intergenic
CCAGCTTCTGGTGGTGGTGG+TGG 0.427315 2.1:+75804976 MS.gene00616:CDS
AAGAGTTGTTAACGTTGAAA+TGG 0.427409 2.1:-75805134 None:intergenic
GAGGGAGAAGAGTTTGAAGA+AGG 0.435751 2.1:-75803881 None:intergenic
GGTGGTGGTGTTGAATTTCA+AGG 0.437658 2.1:+75804994 MS.gene00616:CDS
CAGATCTGGTGGCAATTGCC+AGG 0.437859 2.1:-75803976 None:intergenic
TCATCATTAGGTTGAGGTTG+AGG 0.438959 2.1:-75805210 None:intergenic
CTGATGAACGCTGATCAAAT+TGG 0.440741 2.1:+75804493 MS.gene00616:CDS
GGGATGGGGATGAATATTGG+TGG 0.440791 2.1:-75803744 None:intergenic
GAAATTGGCTGGGATTGTTC+CGG 0.446496 2.1:-75804278 None:intergenic
GGCGAAGGTGACGGTGGTGG+AGG 0.448389 2.1:+75804670 MS.gene00616:CDS
GGCTGTATCTGATTCCAACT+TGG 0.449042 2.1:+75805074 MS.gene00616:CDS
ACAGCCATGTGGGCTACAGC+GGG 0.455998 2.1:+75804772 MS.gene00616:CDS
GGAGCTTCTTCTTCTTCTGT+AGG 0.458812 2.1:+75804703 MS.gene00616:CDS
ACCAACACGCTCCTCTGTTA+GGG 0.462020 2.1:+75804425 MS.gene00616:CDS
GGTGATGGCCTTCCATCAAA+AGG 0.462353 2.1:-75803827 None:intergenic
AATGTTCCAGCTTCTGGTGG+TGG 0.463360 2.1:+75804970 MS.gene00616:CDS
CTCAGTTACATTTCATGCCA+AGG 0.466286 2.1:+75804944 MS.gene00616:CDS
CAAAACAAATCACCAACAAC+AGG 0.468328 2.1:+75804639 MS.gene00616:CDS
CCATTCAATTGTTTCACCAT+CGG 0.476071 2.1:-75804212 None:intergenic
TTGAGCGTTGAGAAATTGGC+TGG 0.476634 2.1:-75804289 None:intergenic
ACACCTCATTGATGCTTCCC+TGG 0.479655 2.1:+75803958 MS.gene00616:CDS
GCTTCTTCAACAAGCGGAGC+CGG 0.481221 2.1:+75804234 MS.gene00616:CDS
TCGACCGTCAAATATGTTAC+CGG 0.485929 2.1:+75804747 MS.gene00616:CDS
ATCATCGCTGCCACCGGCAC+CGG 0.486326 2.1:+75804259 MS.gene00616:CDS
ATGGGCGAAGGTGACGGTGG+TGG 0.487166 2.1:+75804667 MS.gene00616:CDS
AGGATGATTCTTGTTGTTGG+TGG 0.493938 2.1:-75803769 None:intergenic
ATGTGGGCTACAGCGGGGAG+TGG 0.494234 2.1:+75804778 MS.gene00616:CDS
GCTGTATCTGATTCCAACTT+GGG 0.503244 2.1:+75805075 MS.gene00616:CDS
AGGATTTGCAACCGCGGTGA+CGG 0.503756 2.1:-75804071 None:intergenic
ACGAGAATTACATGAGAAAA+CGG 0.506668 2.1:+75804530 MS.gene00616:CDS
CTTAATGCTTATACAAGAGC+TGG 0.507717 2.1:+75805111 MS.gene00616:CDS
CATGTTCAGTCTCAAAATCA+AGG 0.508213 2.1:+75804898 MS.gene00616:CDS
AGCAGCAAACATACCCAAGT+TGG 0.510675 2.1:-75805088 None:intergenic
ACCATCACAGCATTTGGGAT+TGG 0.511721 2.1:+75805053 MS.gene00616:CDS
GTTCCAGCTTCTGGTGGTGG+TGG 0.513092 2.1:+75804973 MS.gene00616:CDS
CCGGCGATCATCGCTGCCAC+CGG 0.513461 2.1:+75804253 MS.gene00616:CDS
CAAAAGTTGACGGCCGTGGC+CGG 0.517328 2.1:+75804122 MS.gene00616:CDS
CCGGTGGCAGCGATGATCGC+CGG 0.518014 2.1:-75804253 None:intergenic
CAAAATGAAAGTGGCGGTGA+TGG 0.520198 2.1:+75804589 MS.gene00616:CDS
GTTGCTGTTGCTGTTGTTGG+TGG 0.522829 2.1:-75804465 None:intergenic
AGAAGGTTGAATATTAGAGA+TGG 0.523303 2.1:-75803864 None:intergenic
TGGTACGCAGCTTCTTCGTA+AGG 0.525067 2.1:-75804406 None:intergenic
TTCAATGTTCCAGCTTCTGG+TGG 0.527514 2.1:+75804967 MS.gene00616:CDS
CACCAACAACAGGATGATAT+GGG 0.530307 2.1:+75804649 MS.gene00616:CDS
CCGATGGTGAAACAATTGAA+TGG 0.532182 2.1:+75804212 MS.gene00616:CDS
GCATTCGAATTCTCCGGCCA+CGG 0.535337 2.1:-75804135 None:intergenic
TGCTGATGATGAGAATTTGA+AGG 0.536098 2.1:-75803794 None:intergenic
TCGCCGGCATTCGAATTCTC+CGG 0.537835 2.1:-75804141 None:intergenic
AACAACAATCAAAATGAAAG+TGG 0.537872 2.1:+75804580 MS.gene00616:CDS
CACACAAAAGTTGACGGCCG+TGG 0.538934 2.1:+75804118 MS.gene00616:CDS
TGGAAGAGGAGGTTCAGATC+TGG 0.540562 2.1:-75803990 None:intergenic
TGTTGTTGGTGGTGTTGATG+AGG 0.542903 2.1:-75804454 None:intergenic
ACAGTGTAGAGCCGCTGCTA+CGG 0.544103 2.1:-75804321 None:intergenic
GACAGGCACACAAAAGTTGA+CGG 0.553080 2.1:+75804112 MS.gene00616:CDS
TTGTTGGATACATCTGGAAG+AGG 0.554700 2.1:-75804004 None:intergenic
ACTCCCCGCTGTAGCCCACA+TGG 0.560090 2.1:-75804776 None:intergenic
GTGATGGCCTTCCATCAAAA+GGG 0.560570 2.1:-75803826 None:intergenic
AACAGCCATGTGGGCTACAG+CGG 0.560758 2.1:+75804771 MS.gene00616:CDS
GTTGCCGGTAACATATTTGA+CGG 0.564703 2.1:-75804751 None:intergenic
CAAAACGATCCACCAAGGAC+AGG 0.572544 2.1:+75804095 MS.gene00616:CDS
GATGATATGGGCGAAGGTGA+CGG 0.576236 2.1:+75804661 MS.gene00616:CDS
TGGGATTGTTCCGGTGCCGG+TGG 0.577716 2.1:-75804269 None:intergenic
ATTTGCAACCGCGGTGACGG+CGG 0.577827 2.1:-75804068 None:intergenic
GTTGAATTTCAAGGTGGACG+TGG 0.582295 2.1:+75805003 MS.gene00616:CDS
ACTCTTGCTGCACATGTCGC+CGG 0.582995 2.1:-75804157 None:intergenic
GTTGCAGCACCACCACTCAC+TGG 0.588807 2.1:-75804820 None:intergenic
TTCTCATCATCAGCAGCTTG+TGG 0.593589 2.1:+75803802 MS.gene00616:CDS
TGGCCGGAGAATTCGAATGC+CGG 0.599117 2.1:+75804138 MS.gene00616:CDS
GAGATGGAGCCCATAATGAA+AGG 0.602222 2.1:-75803848 None:intergenic
GAGCCCATAATGAAAGGTGA+TGG 0.605278 2.1:-75803842 None:intergenic
TTGGATACATCTGGAAGAGG+AGG 0.605386 2.1:-75804001 None:intergenic
TGAATGGCTTCTTCAACAAG+CGG 0.607294 2.1:+75804228 MS.gene00616:CDS
ATGATGAGAATTTGAAGGTG+AGG 0.608155 2.1:-75803789 None:intergenic
GGCATGAAATGTAACTGAGA+GGG 0.612083 2.1:-75804940 None:intergenic
GCCAATCCCAAATGCTGTGA+TGG 0.613902 2.1:-75805054 None:intergenic
GAAGCTGGAACATTGAACCT+TGG 0.618817 2.1:-75804961 None:intergenic
GAGTGGAAGTACAATTTGGA+TGG 0.623430 2.1:+75804795 MS.gene00616:CDS
CAAAACAACAAGCAGAAGAG+AGG 0.623869 2.1:+75803719 MS.gene00616:CDS
GATATGGGCGAAGGTGACGG+TGG 0.625298 2.1:+75804664 MS.gene00616:CDS
AGATCTGGTGGCAATTGCCA+GGG 0.625421 2.1:-75803975 None:intergenic
CCTCAACCTAATGATGAGAG+TGG 0.625980 2.1:+75805216 MS.gene00616:CDS
CAACAGGATGATATGGGCGA+AGG 0.628122 2.1:+75804655 MS.gene00616:CDS
AACTTCAACCGCCGTCACCG+CGG 0.629301 2.1:+75804060 MS.gene00616:CDS
GAAAGTGGCGGTGATGGTGA+TGG 0.632003 2.1:+75804595 MS.gene00616:CDS
AAGAGGAGGTTCAGATCTGG+TGG 0.633145 2.1:-75803987 None:intergenic
AACGTCTCACTCCGTAGCAG+CGG 0.639266 2.1:+75804310 MS.gene00616:CDS
ACCCTAACAGAGGAGCGTGT+TGG 0.640297 2.1:-75804426 None:intergenic
TGAGCGTTGAGAAATTGGCT+GGG 0.640396 2.1:-75804288 None:intergenic
GAATTAGGCCACAAATCCGA+TGG 0.643115 2.1:+75804196 MS.gene00616:CDS
AGCATCAATGAGGTGTTGAG+TGG 0.643841 2.1:-75803951 None:intergenic
CTGAGAGGAGCTGATTTCGA+CGG 0.644392 2.1:-75804352 None:intergenic
AACAATCAAAATGAAAGTGG+CGG 0.649002 2.1:+75804583 MS.gene00616:CDS
GCATCAATGAGGTGTTGAGT+GGG 0.650961 2.1:-75803950 None:intergenic
GTTACCGGCAACAGCCATGT+GGG 0.654057 2.1:+75804762 MS.gene00616:CDS
TGTTACCGGCAACAGCCATG+TGG 0.658913 2.1:+75804761 MS.gene00616:CDS
GGTAGTAGATAGTCATTGTG+AGG 0.659915 2.1:-75805256 None:intergenic
GAATTTCAAGGTGGACGTGG+TGG 0.660159 2.1:+75805006 MS.gene00616:CDS
TGGCATGAAATGTAACTGAG+AGG 0.665182 2.1:-75804941 None:intergenic
TCCTCCAAAACGATCCACCA+AGG 0.665860 2.1:+75804090 MS.gene00616:CDS
CAGCCATGTGGGCTACAGCG+GGG 0.667527 2.1:+75804773 MS.gene00616:CDS
AGAGGAGCTGATTTCGACGG+CGG 0.670392 2.1:-75804349 None:intergenic
AGACACAGACACAAAACATG+TGG 0.683636 2.1:+75804863 MS.gene00616:CDS
TTGCCAGGGAAGCATCAATG+AGG 0.690178 2.1:-75803961 None:intergenic
ATTTGGATGGTACCAGTGAG+TGG 0.695345 2.1:+75804808 MS.gene00616:CDS
TGAGGATGAAACCCTAACAG+AGG 0.710378 2.1:-75804436 None:intergenic
TGGATGGTACCAGTGAGTGG+TGG 0.715883 2.1:+75804811 MS.gene00616:CDS
AGCGCGCTATGAAAACTGAG+AGG 0.721312 2.1:-75804367 None:intergenic
GGTGGTGTTGAATTTCAAGG+TGG 0.744371 2.1:+75804997 MS.gene00616:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTTGTTTTGAAACTTTT+GGG - chr2.1:75804631-75804650 None:intergenic 20.0%
! AACAACAATCAAAATGAAAG+TGG + chr2.1:75804580-75804599 MS.gene00616:CDS 25.0%
! TTGAAGAAAATGGTTGAATT+TGG - chr2.1:75804042-75804061 None:intergenic 25.0%
!!! GTGATTTGTTTTGAAACTTT+TGG - chr2.1:75804632-75804651 None:intergenic 25.0%
AACAATCAAAATGAAAGTGG+CGG + chr2.1:75804583-75804602 MS.gene00616:CDS 30.0%
AAGAGTTGTTAACGTTGAAA+TGG - chr2.1:75805137-75805156 None:intergenic 30.0%
ACGAGAATTACATGAGAAAA+CGG + chr2.1:75804530-75804549 MS.gene00616:CDS 30.0%
AGAAGGTTGAATATTAGAGA+TGG - chr2.1:75803867-75803886 None:intergenic 30.0%
TCAACAACATTCAATGTCAT+TGG + chr2.1:75805173-75805192 MS.gene00616:CDS 30.0%
! TCTGTAGGTAATTCTTTTTC+TGG + chr2.1:75804718-75804737 MS.gene00616:CDS 30.0%
AGCTGAAGAAGAAGAAGAAT+TGG - chr2.1:75803906-75803925 None:intergenic 35.0%
ATGATGAGAATTTGAAGGTG+AGG - chr2.1:75803792-75803811 None:intergenic 35.0%
CAAAACAAATCACCAACAAC+AGG + chr2.1:75804639-75804658 MS.gene00616:CDS 35.0%
CATGTTCAGTCTCAAAATCA+AGG + chr2.1:75804898-75804917 MS.gene00616:CDS 35.0%
CCATTCAATTGTTTCACCAT+CGG - chr2.1:75804215-75804234 None:intergenic 35.0%
CTTAATGCTTATACAAGAGC+TGG + chr2.1:75805111-75805130 MS.gene00616:CDS 35.0%
GAAGAAGAAGAAGAATTGGA+GGG - chr2.1:75803902-75803921 None:intergenic 35.0%
TATCAACCATCACAGCATTT+GGG + chr2.1:75805048-75805067 MS.gene00616:CDS 35.0%
TGAAGAAGAAGAAGAATTGG+AGG - chr2.1:75803903-75803922 None:intergenic 35.0%
! TGCTGATGATGAGAATTTGA+AGG - chr2.1:75803797-75803816 None:intergenic 35.0%
! TTGGTTGTTGTTGTTGTTGT+TGG - chr2.1:75804023-75804042 None:intergenic 35.0%
! TTGTTGTTGTTGGATACATC+TGG - chr2.1:75804013-75804032 None:intergenic 35.0%
!!! TGATTTTGAGACTGAACATG+AGG - chr2.1:75804898-75804917 None:intergenic 35.0%
AAGGCCATCACCTTTCATTA+TGG + chr2.1:75803838-75803857 MS.gene00616:CDS 40.0%
AGACACAGACACAAAACATG+TGG + chr2.1:75804863-75804882 MS.gene00616:CDS 40.0%
AGGCCATCACCTTTCATTAT+GGG + chr2.1:75803839-75803858 MS.gene00616:CDS 40.0%
CAAAACAACAAGCAGAAGAG+AGG + chr2.1:75803719-75803738 MS.gene00616:CDS 40.0%
CACCAACAACAGGATGATAT+GGG + chr2.1:75804649-75804668 MS.gene00616:CDS 40.0%
CCGATGGTGAAACAATTGAA+TGG + chr2.1:75804212-75804231 MS.gene00616:CDS 40.0%
CTCAGTTACATTTCATGCCA+AGG + chr2.1:75804944-75804963 MS.gene00616:CDS 40.0%
CTGATGAACGCTGATCAAAT+TGG + chr2.1:75804493-75804512 MS.gene00616:CDS 40.0%
GACTGAACATGAGGTGTAAA+AGG - chr2.1:75804889-75804908 None:intergenic 40.0%
GCGGTTGAAGTTGAAGAAAA+TGG - chr2.1:75804052-75804071 None:intergenic 40.0%
GCTGTATCTGATTCCAACTT+GGG + chr2.1:75805075-75805094 MS.gene00616:CDS 40.0%
GGCATGAAATGTAACTGAGA+GGG - chr2.1:75804943-75804962 None:intergenic 40.0%
GTATCAACCATCACAGCATT+TGG + chr2.1:75805047-75805066 MS.gene00616:CDS 40.0%
GTTGCCGGTAACATATTTGA+CGG - chr2.1:75804754-75804773 None:intergenic 40.0%
TCACCAACAACAGGATGATA+TGG + chr2.1:75804648-75804667 MS.gene00616:CDS 40.0%
TCGACCGTCAAATATGTTAC+CGG + chr2.1:75804747-75804766 MS.gene00616:CDS 40.0%
TCTTCTCCACTCTCATCATT+AGG - chr2.1:75805225-75805244 None:intergenic 40.0%
TGAATGGCTTCTTCAACAAG+CGG + chr2.1:75804228-75804247 MS.gene00616:CDS 40.0%
TGGCATGAAATGTAACTGAG+AGG - chr2.1:75804944-75804963 None:intergenic 40.0%
TTCCAGTTGACCAGAGAATT+AGG + chr2.1:75804181-75804200 MS.gene00616:CDS 40.0%
TTGTTGGATACATCTGGAAG+AGG - chr2.1:75804007-75804026 None:intergenic 40.0%
TTGTTTCACCATCGGATTTG+TGG - chr2.1:75804207-75804226 None:intergenic 40.0%
! GAGTGGAAGTACAATTTGGA+TGG + chr2.1:75804795-75804814 MS.gene00616:CDS 40.0%
! GTGAGGATGATTCTTGTTGT+TGG - chr2.1:75803775-75803794 None:intergenic 40.0%
!! AGGATGATTCTTGTTGTTGG+TGG - chr2.1:75803772-75803791 None:intergenic 40.0%
!! ATGATTCTTGTTGTTGGTGG+TGG - chr2.1:75803769-75803788 None:intergenic 40.0%
!! TCATCATTAGGTTGAGGTTG+AGG - chr2.1:75805213-75805232 None:intergenic 40.0%
!! TGATTCTTGTTGTTGGTGGT+GGG - chr2.1:75803768-75803787 None:intergenic 40.0%
AGCAGCAAACATACCCAAGT+TGG - chr2.1:75805091-75805110 None:intergenic 45.0%
AGGTTCAATGTTCCAGCTTC+TGG + chr2.1:75804964-75804983 MS.gene00616:CDS 45.0%
ATTTGGATGGTACCAGTGAG+TGG + chr2.1:75804808-75804827 MS.gene00616:CDS 45.0%
CAAAATGAAAGTGGCGGTGA+TGG + chr2.1:75804589-75804608 MS.gene00616:CDS 45.0%
CCTCAACCTAATGATGAGAG+TGG + chr2.1:75805216-75805235 MS.gene00616:CDS 45.0%
GAAATTGGCTGGGATTGTTC+CGG - chr2.1:75804281-75804300 None:intergenic 45.0%
GAAGCTGGAACATTGAACCT+TGG - chr2.1:75804964-75804983 None:intergenic 45.0%
GAATTAGGCCACAAATCCGA+TGG + chr2.1:75804196-75804215 MS.gene00616:CDS 45.0%
GACAGGCACACAAAAGTTGA+CGG + chr2.1:75804112-75804131 MS.gene00616:CDS 45.0%
GAGATGGAGCCCATAATGAA+AGG - chr2.1:75803851-75803870 None:intergenic 45.0%
GAGCCCATAATGAAAGGTGA+TGG - chr2.1:75803845-75803864 None:intergenic 45.0%
GAGGGAGAAGAGTTTGAAGA+AGG - chr2.1:75803884-75803903 None:intergenic 45.0%
GGACGTGGTGGTTTACAATT+GGG + chr2.1:75805018-75805037 MS.gene00616:CDS 45.0%
GGAGCTTCTTCTTCTTCTGT+AGG + chr2.1:75804703-75804722 MS.gene00616:CDS 45.0%
GGATTTGTGGCCTAATTCTC+TGG - chr2.1:75804194-75804213 None:intergenic 45.0%
GGCTGTATCTGATTCCAACT+TGG + chr2.1:75805074-75805093 MS.gene00616:CDS 45.0%
GTGATGGCCTTCCATCAAAA+GGG - chr2.1:75803829-75803848 None:intergenic 45.0%
GTTGAATTTCAAGGTGGACG+TGG + chr2.1:75805003-75805022 MS.gene00616:CDS 45.0%
TGAGGATGAAACCCTAACAG+AGG - chr2.1:75804439-75804458 None:intergenic 45.0%
TGGACGTGGTGGTTTACAAT+TGG + chr2.1:75805017-75805036 MS.gene00616:CDS 45.0%
TTCAATGTTCCAGCTTCTGG+TGG + chr2.1:75804967-75804986 MS.gene00616:CDS 45.0%
TTCTCATCATCAGCAGCTTG+TGG + chr2.1:75803802-75803821 MS.gene00616:CDS 45.0%
TTGGATACATCTGGAAGAGG+AGG - chr2.1:75804004-75804023 None:intergenic 45.0%
! AACTTTTGTGTGCCTGTCCT+TGG - chr2.1:75804110-75804129 None:intergenic 45.0%
! ACCATCACAGCATTTGGGAT+TGG + chr2.1:75805053-75805072 MS.gene00616:CDS 45.0%
! CCACTCTCATCATTAGGTTG+AGG - chr2.1:75805219-75805238 None:intergenic 45.0%
! GGTGGTGGTGTTGAATTTCA+AGG + chr2.1:75804994-75805013 MS.gene00616:CDS 45.0%
! GGTGGTGTTGAATTTCAAGG+TGG + chr2.1:75804997-75805016 MS.gene00616:CDS 45.0%
! GTTGTTGCTGTTGCTGTTGT+TGG - chr2.1:75804471-75804490 None:intergenic 45.0%
! TTGTGGTCCCTTTTGATGGA+AGG + chr2.1:75803819-75803838 MS.gene00616:CDS 45.0%
!! AGCATCAATGAGGTGTTGAG+TGG - chr2.1:75803954-75803973 None:intergenic 45.0%
!! GACGTTGAGCGTTGAGAAAT+TGG - chr2.1:75804296-75804315 None:intergenic 45.0%
!! GATTCTTGTTGTTGGTGGTG+GGG - chr2.1:75803767-75803786 None:intergenic 45.0%
!! GCATCAATGAGGTGTTGAGT+GGG - chr2.1:75803953-75803972 None:intergenic 45.0%
!! TGAGCGTTGAGAAATTGGCT+GGG - chr2.1:75804291-75804310 None:intergenic 45.0%
!! TGTTGTTGGTGGTGTTGATG+AGG - chr2.1:75804457-75804476 None:intergenic 45.0%
!! TTGAGCGTTGAGAAATTGGC+TGG - chr2.1:75804292-75804311 None:intergenic 45.0%
!! TTTTGGAGGATTTGCAACCG+CGG - chr2.1:75804080-75804099 None:intergenic 45.0%
AAGAGGAGGTTCAGATCTGG+TGG - chr2.1:75803990-75804009 None:intergenic 50.0%
AATGTTCCAGCTTCTGGTGG+TGG + chr2.1:75804970-75804989 MS.gene00616:CDS 50.0%
ACACCTCATTGATGCTTCCC+TGG + chr2.1:75803958-75803977 MS.gene00616:CDS 50.0%
ACCAACACGCTCCTCTGTTA+GGG + chr2.1:75804425-75804444 MS.gene00616:CDS 50.0%
AGATCTGGTGGCAATTGCCA+GGG - chr2.1:75803978-75803997 None:intergenic 50.0%
AGCGCGCTATGAAAACTGAG+AGG - chr2.1:75804370-75804389 None:intergenic 50.0%
CAAAACGATCCACCAAGGAC+AGG + chr2.1:75804095-75804114 MS.gene00616:CDS 50.0%
CAACAGGATGATATGGGCGA+AGG + chr2.1:75804655-75804674 MS.gene00616:CDS 50.0%
CGCCCATATCATCCTGTTGT+TGG - chr2.1:75804654-75804673 None:intergenic 50.0%
GAATTTCAAGGTGGACGTGG+TGG + chr2.1:75805006-75805025 MS.gene00616:CDS 50.0%
GATGATATGGGCGAAGGTGA+CGG + chr2.1:75804661-75804680 MS.gene00616:CDS 50.0%
GCCAATCCCAAATGCTGTGA+TGG - chr2.1:75805057-75805076 None:intergenic 50.0%
GGCCTAATTCTCTGGTCAAC+TGG - chr2.1:75804186-75804205 None:intergenic 50.0%
GGGATGGGGATGAATATTGG+TGG - chr2.1:75803747-75803766 None:intergenic 50.0%
GTGGGGATGGGGATGAATAT+TGG - chr2.1:75803750-75803769 None:intergenic 50.0%
TACCAACACGCTCCTCTGTT+AGG + chr2.1:75804424-75804443 MS.gene00616:CDS 50.0%
TCCTCCAAAACGATCCACCA+AGG + chr2.1:75804090-75804109 MS.gene00616:CDS 50.0%
TGGAAGAGGAGGTTCAGATC+TGG - chr2.1:75803993-75804012 None:intergenic 50.0%
TGGTACGCAGCTTCTTCGTA+AGG - chr2.1:75804409-75804428 None:intergenic 50.0%
! CAGCTTGTGGTCCCTTTTGA+TGG + chr2.1:75803815-75803834 MS.gene00616:CDS 50.0%
! CGGGGAGTGGAAGTACAATT+TGG + chr2.1:75804791-75804810 MS.gene00616:CDS 50.0%
! CTGAGAGGAGCTGATTTCGA+CGG - chr2.1:75804355-75804374 None:intergenic 50.0%
! GGTGATGGCCTTCCATCAAA+AGG - chr2.1:75803830-75803849 None:intergenic 50.0%
! GTTGCTGTTGCTGTTGTTGG+TGG - chr2.1:75804468-75804487 None:intergenic 50.0%
! TTTTCATAGCGCGCTTGCAC+TGG + chr2.1:75804375-75804394 MS.gene00616:CDS 50.0%
! TTTTGTGTGCCTGTCCTTGG+TGG - chr2.1:75804107-75804126 None:intergenic 50.0%
!! CTGTCCTTGGTGGATCGTTT+TGG - chr2.1:75804097-75804116 None:intergenic 50.0%
!! TCCTTGGTGGATCGTTTTGG+AGG - chr2.1:75804094-75804113 None:intergenic 50.0%
!! TTGCCAGGGAAGCATCAATG+AGG - chr2.1:75803964-75803983 None:intergenic 50.0%
!! TTGTTGTTGGTGGTGGGGAT+GGG - chr2.1:75803762-75803781 None:intergenic 50.0%
AACGTCTCACTCCGTAGCAG+CGG + chr2.1:75804310-75804329 MS.gene00616:CDS 55.0%
ACAGTGTAGAGCCGCTGCTA+CGG - chr2.1:75804324-75804343 None:intergenic 55.0%
ACTCTTGCTGCACATGTCGC+CGG - chr2.1:75804160-75804179 None:intergenic 55.0%
AGGATTTGCAACCGCGGTGA+CGG - chr2.1:75804074-75804093 None:intergenic 55.0%
CACACAAAAGTTGACGGCCG+TGG + chr2.1:75804118-75804137 MS.gene00616:CDS 55.0%
CAGATCTGGTGGCAATTGCC+AGG - chr2.1:75803979-75803998 None:intergenic 55.0%
GCATTCGAATTCTCCGGCCA+CGG - chr2.1:75804138-75804157 None:intergenic 55.0%
GCTTCTTCAACAAGCGGAGC+CGG + chr2.1:75804234-75804253 MS.gene00616:CDS 55.0%
GTTACCGGCAACAGCCATGT+GGG + chr2.1:75804762-75804781 MS.gene00616:CDS 55.0%
TCGCCGGCATTCGAATTCTC+CGG - chr2.1:75804144-75804163 None:intergenic 55.0%
TGGATGGTACCAGTGAGTGG+TGG + chr2.1:75804811-75804830 MS.gene00616:CDS 55.0%
TGGCCGGAGAATTCGAATGC+CGG + chr2.1:75804138-75804157 MS.gene00616:CDS 55.0%
TGTTACCGGCAACAGCCATG+TGG + chr2.1:75804761-75804780 MS.gene00616:CDS 55.0%
! AACAGCCATGTGGGCTACAG+CGG + chr2.1:75804771-75804790 MS.gene00616:CDS 55.0%
! ACCCTAACAGAGGAGCGTGT+TGG - chr2.1:75804429-75804448 None:intergenic 55.0%
! AGAGGAGCTGATTTCGACGG+CGG - chr2.1:75804352-75804371 None:intergenic 55.0%
! GAAAGTGGCGGTGATGGTGA+TGG + chr2.1:75804595-75804614 MS.gene00616:CDS 55.0%
!! CTTGTTGTTGGTGGTGGGGA+TGG - chr2.1:75803763-75803782 None:intergenic 55.0%
!! TGTTGTTGGTGGTGGGGATG+GGG - chr2.1:75803761-75803780 None:intergenic 55.0%
AACTTCAACCGCCGTCACCG+CGG + chr2.1:75804060-75804079 MS.gene00616:CDS 60.0%
CAAAAGTTGACGGCCGTGGC+CGG + chr2.1:75804122-75804141 MS.gene00616:CDS 60.0%
GTAGCCCACATGGCTGTTGC+CGG - chr2.1:75804769-75804788 None:intergenic 60.0%
GTTGCAGCACCACCACTCAC+TGG - chr2.1:75804823-75804842 None:intergenic 60.0%
! ACAGCCATGTGGGCTACAGC+GGG + chr2.1:75804772-75804791 MS.gene00616:CDS 60.0%
! ATTTGCAACCGCGGTGACGG+CGG - chr2.1:75804071-75804090 None:intergenic 60.0%
! GATATGGGCGAAGGTGACGG+TGG + chr2.1:75804664-75804683 MS.gene00616:CDS 60.0%
!! GTTCCAGCTTCTGGTGGTGG+TGG + chr2.1:75804973-75804992 MS.gene00616:CDS 60.0%
ACTCCCCGCTGTAGCCCACA+TGG - chr2.1:75804779-75804798 None:intergenic 65.0%
ATCATCGCTGCCACCGGCAC+CGG + chr2.1:75804259-75804278 MS.gene00616:CDS 65.0%
CCACCACCACCACCAGAAGC+TGG - chr2.1:75804979-75804998 None:intergenic 65.0%
GGCTGGGATTGTTCCGGTGC+CGG - chr2.1:75804275-75804294 None:intergenic 65.0%
! ATGGGCGAAGGTGACGGTGG+TGG + chr2.1:75804667-75804686 MS.gene00616:CDS 65.0%
! ATGTGGGCTACAGCGGGGAG+TGG + chr2.1:75804778-75804797 MS.gene00616:CDS 65.0%
! CAGCCATGTGGGCTACAGCG+GGG + chr2.1:75804773-75804792 MS.gene00616:CDS 65.0%
! TGGGATTGTTCCGGTGCCGG+TGG - chr2.1:75804272-75804291 None:intergenic 65.0%
!! CCAGCTTCTGGTGGTGGTGG+TGG + chr2.1:75804976-75804995 MS.gene00616:CDS 65.0%
!! GAAGGTGACGGTGGTGGAGG+AGG + chr2.1:75804673-75804692 MS.gene00616:CDS 65.0%
!! GCTTCTGGTGGTGGTGGTGG+TGG + chr2.1:75804979-75804998 MS.gene00616:CDS 65.0%
CCGGCGATCATCGCTGCCAC+CGG + chr2.1:75804253-75804272 MS.gene00616:CDS 70.0%
CCGGTGGCAGCGATGATCGC+CGG - chr2.1:75804256-75804275 None:intergenic 70.0%
! GGCGAAGGTGACGGTGGTGG+AGG + chr2.1:75804670-75804689 MS.gene00616:CDS 70.0%
!! GACGGTGGTGGAGGAGGTGG+TGG + chr2.1:75804679-75804698 MS.gene00616:CDS 70.0%
!! GGTGACGGTGGTGGAGGAGG+TGG + chr2.1:75804676-75804695 MS.gene00616:CDS 70.0%
!! GGTGGTGGAGGAGGTGGTGG+TGG + chr2.1:75804682-75804701 MS.gene00616:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 75803701 75805266 75803701 ID=MS.gene00616
chr2.1 mRNA 75803701 75805266 75803701 ID=MS.gene00616.t1;Parent=MS.gene00616
chr2.1 exon 75803701 75805266 75803701 ID=MS.gene00616.t1.exon1;Parent=MS.gene00616.t1
chr2.1 CDS 75803701 75805266 75803701 ID=cds.MS.gene00616.t1;Parent=MS.gene00616.t1
Gene Sequence

>MS.gene00616

ATGGAACTATCAGATTTACAAAACAACAAGCAGAAGAGAGGCACCACCAATATTCATCCCCATCCCCACCACCAACAACAAGAATCATCCTCACCTTCAAATTCTCATCATCAGCAGCTTGTGGTCCCTTTTGATGGAAGGCCATCACCTTTCATTATGGGCTCCATCTCTAATATTCAACCTTCTTCAAACTCTTCTCCCTCCAATTCTTCTTCTTCTTCAGCTTCAGCTTCAGCTTCAGCTTCTGCACCCACTCAACACCTCATTGATGCTTCCCTGGCAATTGCCACCAGATCTGAACCTCCTCTTCCAGATGTATCCAACAACAACAACAACAACCAAATTCAACCATTTTCTTCAACTTCAACCGCCGTCACCGCGGTTGCAAATCCTCCAAAACGATCCACCAAGGACAGGCACACAAAAGTTGACGGCCGTGGCCGGAGAATTCGAATGCCGGCGACATGTGCAGCAAGAGTTTTCCAGTTGACCAGAGAATTAGGCCACAAATCCGATGGTGAAACAATTGAATGGCTTCTTCAACAAGCGGAGCCGGCGATCATCGCTGCCACCGGCACCGGAACAATCCCAGCCAATTTCTCAACGCTCAACGTCTCACTCCGTAGCAGCGGCTCTACACTGTCTGCGCCGCCGTCGAAATCAGCTCCTCTCAGTTTTCATAGCGCGCTTGCACTGGCTCATCATCCTTACGAAGAAGCTGCGTACCAACACGCTCCTCTGTTAGGGTTTCATCCTCATCAACACCACCAACAACAGCAACAGCAACAACAACTGATGAACGCTGATCAAATTGGTGAAGCACTTTCAAACGAGAATTACATGAGAAAACGGTATAGAGAAGATCTTTTCAAAGATGATAACAACAATCAAAATGAAAGTGGCGGTGATGGTGATGGTTCTTCTTCTCCCAAAAGTTTCAAAACAAATCACCAACAACAGGATGATATGGGCGAAGGTGACGGTGGTGGAGGAGGTGGTGGTGGAGCTTCTTCTTCTTCTGTAGGTAATTCTTTTTCTGGACTTCTTCGACCGTCAAATATGTTACCGGCAACAGCCATGTGGGCTACAGCGGGGAGTGGAAGTACAATTTGGATGGTACCAGTGAGTGGTGGTGCTGCAACTTCTTCAAATTCAGAGACACAGACACAGACACAAAACATGTGGCCTTTTACACCTCATGTTCAGTCTCAAAATCAAGGCAACGCTGCTGCTGTAACTCCCTCTCAGTTACATTTCATGCCAAGGTTCAATGTTCCAGCTTCTGGTGGTGGTGGTGGTGGTGTTGAATTTCAAGGTGGACGTGGTGGTTTACAATTGGGTTCAGCGTATCAACCATCACAGCATTTGGGATTGGCTGTATCTGATTCCAACTTGGGTATGTTTGCTGCTCTTAATGCTTATACAAGAGCTGGCCATTTCAACGTTAACAACTCTTCTGATCATCATCATCATCAACAACATTCAATGTCATTGGACTCTCATTCTCAACCTCAACCTCAACCTAATGATGAGAGTGGAGAAGATGCTCCTAATACCTCACAATGA
Protein sequence

>MS.gene00616.t1

MELSDLQNNKQKRGTTNIHPHPHHQQQESSSPSNSHHQQLVVPFDGRPSPFIMGSISNIQPSSNSSPSNSSSSSASASASASAPTQHLIDASLAIATRSEPPLPDVSNNNNNNQIQPFSSTSTAVTAVANPPKRSTKDRHTKVDGRGRRIRMPATCAARVFQLTRELGHKSDGETIEWLLQQAEPAIIAATGTGTIPANFSTLNVSLRSSGSTLSAPPSKSAPLSFHSALALAHHPYEEAAYQHAPLLGFHPHQHHQQQQQQQQLMNADQIGEALSNENYMRKRYREDLFKDDNNNQNESGGDGDGSSSPKSFKTNHQQQDDMGEGDGGGGGGGGASSSSVGNSFSGLLRPSNMLPATAMWATAGSGSTIWMVPVSGGAATSSNSETQTQTQNMWPFTPHVQSQNQGNAAAVTPSQLHFMPRFNVPASGGGGGGVEFQGGRGGLQLGSAYQPSQHLGLAVSDSNLGMFAALNAYTRAGHFNVNNSSDHHHHQQHSMSLDSHSQPQPQPNDESGEDAPNTSQ