AlfalfaGEDB | MS.gene006450

M. sativa cultivar XinJiangDaYe / MS.gene006450

KEGG: -
GO: -
PFAM: PF04844.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006450.t1	XP_024626005.1	88.6	229	21	3	1	228	1	225	8.20E-63	250.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006450.t1	O23341	55.0	60	27	0	119	178	109	168	2.1e-11	70.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006450.t1	A0A396K274	88.6	229	21	3	1	228	1	225	5.9e-63	250.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene006450.t1	TF	OFP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006450.t1	MTR_1g103640	90.367	218	16	3	1	217	1	214	8.21e-120	340
MS.gene006450.t1	MTR_1g072110	47.794	136	49	2	68	182	102	236	4.21e-35	125
MS.gene006450.t1	MTR_7g095150	42.604	169	61	6	34	181	48	201	4.57e-32	116
MS.gene006450.t1	MTR_2g102140	38.356	73	42	1	118	187	208	280	2.72e-12	65.1
MS.gene006450.t1	MTR_1g111640	33.600	125	68	5	55	178	89	199	1.29e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006450.t1	AT1G05420	45.299	117	60	2	71	184	107	222	3.42e-24	96.3
MS.gene006450.t1	AT1G05420	45.299	117	60	2	71	184	123	238	3.66e-24	96.7
MS.gene006450.t1	AT2G32100	37.241	145	60	2	69	182	94	238	9.44e-22	90.5
MS.gene006450.t1	AT4G14860	54.545	66	30	0	121	186	111	176	9.71e-18	78.2
MS.gene006450.t1	AT1G79960	36.170	94	52	1	87	180	175	260	4.36e-12	64.7

Find 66 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAGGGTTTACAGAGTATTT+TGG	0.228255	1.2:-77187682	None:intergenic
CTCTTCACACTACCTCTTCT+TGG	0.295616	1.2:-77187655	None:intergenic
CGTAGAAATAGTCTTCTTCT+TGG	0.335486	1.2:-77187498	None:intergenic
CTTGGTCGTGGTGGTGGTGG+TGG	0.362617	1.2:-77187480	None:intergenic
TATCGCCACGGTAGTTTCTC+AGG	0.363292	1.2:+77187960	MS.gene006450:CDS
AGCTGTTTAGAGAATAGTTT+TGG	0.372531	1.2:-77187340	None:intergenic
TGTTAGATGTTACTAATGAT+TGG	0.384737	1.2:+77187755	MS.gene006450:CDS
GAGAAACTACCGTGGCGATA+TGG	0.397249	1.2:-77187957	None:intergenic
TGAGGTTGGGAGAGAATTGT+AGG	0.399313	1.2:-77187391	None:intergenic
AGGAGACTCTGTGATGGAAT+TGG	0.407788	1.2:-77187599	None:intergenic
TGGCGTTGGTTATTGAGCTT+TGG	0.408724	1.2:-77187937	None:intergenic
GTTAGATGTTACTAATGATT+GGG	0.414482	1.2:+77187756	MS.gene006450:CDS
GTGGTAGTGTGTTGTGGATG+AGG	0.449355	1.2:-77187409	None:intergenic
CAAGGTAGAGTGTCGTTAGA+AGG	0.451760	1.2:-77187619	None:intergenic
TAGTGTGTTGTGGATGAGGT+TGG	0.452668	1.2:-77187405	None:intergenic
TGTGATGGAATTGGAGCTGC+CGG	0.453476	1.2:-77187590	None:intergenic
GTGATGGAATTGGAGCTGCC+GGG	0.463677	1.2:-77187589	None:intergenic
GGAGAATGATGAAGAGGAGG+AGG	0.466907	1.2:-77187884	None:intergenic
GGGTGAAGGTGGTATTGAAA+TGG	0.466993	1.2:-77187459	None:intergenic
GAACGGAGGAAATCAACATA+AGG	0.476551	1.2:-77187700	None:intergenic
CTTCTTGGTCGTGGTGGTGG+TGG	0.481550	1.2:-77187483	None:intergenic
TGATTTCCTCCGTTCTATGC+AGG	0.483346	1.2:+77187708	MS.gene006450:CDS
GTGGTGGTGGTGGGTGAAGG+TGG	0.483521	1.2:-77187470	None:intergenic
TTGGTCGTGGTGGTGGTGGT+GGG	0.488030	1.2:-77187479	None:intergenic
CTTCTTCTTGGTCGTGGTGG+TGG	0.488768	1.2:-77187486	None:intergenic
AGTGTGTTGTGGATGAGGTT+GGG	0.497151	1.2:-77187404	None:intergenic
AATAGTCTTCTTCTTGGTCG+TGG	0.507672	1.2:-77187492	None:intergenic
TGATGGAATTGGAGCTGCCG+GGG	0.520934	1.2:-77187588	None:intergenic
AAGAAGAGGTAGTGTGAAGA+GGG	0.521773	1.2:+77187657	MS.gene006450:CDS
TGGAAAGTGCAAGGTGATGA+TGG	0.523328	1.2:-77187439	None:intergenic
AGGTTCTTCTTCTCCTCCCC+CGG	0.527466	1.2:+77187571	MS.gene006450:CDS
CACCACGGAGCTTCCACCAC+CGG	0.528976	1.2:+77187522	MS.gene006450:CDS
CGGTAGTTTCTCAGGGAAGT+TGG	0.530160	1.2:+77187968	MS.gene006450:CDS
GGAGGAGAATGATGAAGAGG+AGG	0.534241	1.2:-77187887	None:intergenic
AACGGAGGAAATCAACATAA+GGG	0.534785	1.2:-77187699	None:intergenic
TTTGGAGAAGATAGCGGTGG+CGG	0.537055	1.2:-77187919	None:intergenic
AGAGGAGGAGAATGATGAAG+AGG	0.539046	1.2:-77187890	None:intergenic
ACAGAAGAAAAGTCCGGTGG+TGG	0.542301	1.2:-77187535	None:intergenic
TAGCGGTGGCGGAGATGAAG+AGG	0.545559	1.2:-77187908	None:intergenic
GATGAAGTGGTAGTGTGTTG+TGG	0.564172	1.2:-77187415	None:intergenic
GAATCATTTCCTGCATAGAA+CGG	0.567536	1.2:-77187717	None:intergenic
AAGATAAAATATCAACAAGA+AGG	0.574451	1.2:-77187861	None:intergenic
CAAGAAGAGGTAGTGTGAAG+AGG	0.577676	1.2:+77187656	MS.gene006450:CDS
CTTGGTATCAACGAGTTGCA+AGG	0.582336	1.2:-77187637	None:intergenic
CTTCTGTCTTCGCGTCTCAG+AGG	0.595550	1.2:+77187551	MS.gene006450:CDS
GATGGAATTGGAGCTGCCGG+GGG	0.596484	1.2:-77187587	None:intergenic
CGGTGGCGGAGATGAAGAGG+AGG	0.598051	1.2:-77187905	None:intergenic
CTACCGTGGCGATATGGCGT+TGG	0.601430	1.2:-77187951	None:intergenic
AGTCTTCTTCTTGGTCGTGG+TGG	0.610248	1.2:-77187489	None:intergenic
GAGGTTGGGAGAGAATTGTA+GGG	0.614608	1.2:-77187390	None:intergenic
AGGTGATGATGGTGATGAAG+TGG	0.616233	1.2:-77187428	None:intergenic
GCGAAGACAGAAGAAAAGTC+CGG	0.617433	1.2:-77187541	None:intergenic
ATCGCCACGGTAGTTTCTCA+GGG	0.618065	1.2:+77187961	MS.gene006450:CDS
TTGAGCTTTGGAGAAGATAG+CGG	0.632157	1.2:-77187925	None:intergenic
ATATACTTGTGAGTATGCGT+AGG	0.648661	1.2:-77187820	None:intergenic
GTTAGAAGGAGACTCTGTGA+TGG	0.651959	1.2:-77187605	None:intergenic
AACTCGTTGATACCAAGAAG+AGG	0.656940	1.2:+77187643	MS.gene006450:CDS
AGCTTTGGAGAAGATAGCGG+TGG	0.665519	1.2:-77187922	None:intergenic
ATTTGAGCTTGAAGAAACAG+AGG	0.670569	1.2:-77187366	None:intergenic
GTCCGGTGGTGGAAGCTCCG+TGG	0.684749	1.2:-77187524	None:intergenic
TCATTTCCTGCATAGAACGG+AGG	0.700476	1.2:-77187714	None:intergenic
AGACTATTTCTACGACACCA+CGG	0.705726	1.2:+77187507	MS.gene006450:CDS
TAACCAACGCCATATCGCCA+CGG	0.709212	1.2:+77187948	MS.gene006450:CDS
GTATTGAAATGGAAAGTGCA+AGG	0.712044	1.2:-77187448	None:intergenic
AAGACAGAAGAAAAGTCCGG+TGG	0.739392	1.2:-77187538	None:intergenic
ACTTCCCTGAGAAACTACCG+TGG	0.784562	1.2:-77187965	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTAAAAATATTAAATGTCA+AGG	+	chr1.2:77187991-77188010	MS.gene006450:CDS	15.0%
!!	AAGATAAAATATCAACAAGA+AGG	-	chr1.2:77187864-77187883	None:intergenic	20.0%
!	GTTAGATGTTACTAATGATT+GGG	+	chr1.2:77187756-77187775	MS.gene006450:CDS	25.0%
!	TGTTAGATGTTACTAATGAT+TGG	+	chr1.2:77187755-77187774	MS.gene006450:CDS	25.0%
!	AGCTGTTTAGAGAATAGTTT+TGG	-	chr1.2:77187343-77187362	None:intergenic	30.0%
!!	TAAGGGTTTACAGAGTATTT+TGG	-	chr1.2:77187685-77187704	None:intergenic	30.0%
	AACGGAGGAAATCAACATAA+GGG	-	chr1.2:77187702-77187721	None:intergenic	35.0%
	ATATACTTGTGAGTATGCGT+AGG	-	chr1.2:77187823-77187842	None:intergenic	35.0%
	ATTTGAGCTTGAAGAAACAG+AGG	-	chr1.2:77187369-77187388	None:intergenic	35.0%
	CGTAGAAATAGTCTTCTTCT+TGG	-	chr1.2:77187501-77187520	None:intergenic	35.0%
	GAATCATTTCCTGCATAGAA+CGG	-	chr1.2:77187720-77187739	None:intergenic	35.0%
!	GTATTGAAATGGAAAGTGCA+AGG	-	chr1.2:77187451-77187470	None:intergenic	35.0%
	AACTCGTTGATACCAAGAAG+AGG	+	chr1.2:77187643-77187662	MS.gene006450:CDS	40.0%
	AAGAAGAGGTAGTGTGAAGA+GGG	+	chr1.2:77187657-77187676	MS.gene006450:CDS	40.0%
	AATAGTCTTCTTCTTGGTCG+TGG	-	chr1.2:77187495-77187514	None:intergenic	40.0%
	GAACGGAGGAAATCAACATA+AGG	-	chr1.2:77187703-77187722	None:intergenic	40.0%
!!	AGACTATTTCTACGACACCA+CGG	+	chr1.2:77187507-77187526	MS.gene006450:CDS	40.0%
!!	TTGAGCTTTGGAGAAGATAG+CGG	-	chr1.2:77187928-77187947	None:intergenic	40.0%
	AAGACAGAAGAAAAGTCCGG+TGG	-	chr1.2:77187541-77187560	None:intergenic	45.0%
	AGAGGAGGAGAATGATGAAG+AGG	-	chr1.2:77187893-77187912	None:intergenic	45.0%
	AGTGTGTTGTGGATGAGGTT+GGG	-	chr1.2:77187407-77187426	None:intergenic	45.0%
	CAAGAAGAGGTAGTGTGAAG+AGG	+	chr1.2:77187656-77187675	MS.gene006450:CDS	45.0%
	CTCTTCACACTACCTCTTCT+TGG	-	chr1.2:77187658-77187677	None:intergenic	45.0%
	CTTGGTATCAACGAGTTGCA+AGG	-	chr1.2:77187640-77187659	None:intergenic	45.0%
	GAGGTTGGGAGAGAATTGTA+GGG	-	chr1.2:77187393-77187412	None:intergenic	45.0%
	GCGAAGACAGAAGAAAAGTC+CGG	-	chr1.2:77187544-77187563	None:intergenic	45.0%
	GTTAGAAGGAGACTCTGTGA+TGG	-	chr1.2:77187608-77187627	None:intergenic	45.0%
	TAGTGTGTTGTGGATGAGGT+TGG	-	chr1.2:77187408-77187427	None:intergenic	45.0%
	TCATTTCCTGCATAGAACGG+AGG	-	chr1.2:77187717-77187736	None:intergenic	45.0%
	TGAGGTTGGGAGAGAATTGT+AGG	-	chr1.2:77187394-77187413	None:intergenic	45.0%
	TGATTTCCTCCGTTCTATGC+AGG	+	chr1.2:77187708-77187727	MS.gene006450:CDS	45.0%
!	AGGAGACTCTGTGATGGAAT+TGG	-	chr1.2:77187602-77187621	None:intergenic	45.0%
!	GATGAAGTGGTAGTGTGTTG+TGG	-	chr1.2:77187418-77187437	None:intergenic	45.0%
!	GGGTGAAGGTGGTATTGAAA+TGG	-	chr1.2:77187462-77187481	None:intergenic	45.0%
!	TGGAAAGTGCAAGGTGATGA+TGG	-	chr1.2:77187442-77187461	None:intergenic	45.0%
!!	AGGTGATGATGGTGATGAAG+TGG	-	chr1.2:77187431-77187450	None:intergenic	45.0%
!!	CAAGGTAGAGTGTCGTTAGA+AGG	-	chr1.2:77187622-77187641	None:intergenic	45.0%
!!	TGGCGTTGGTTATTGAGCTT+TGG	-	chr1.2:77187940-77187959	None:intergenic	45.0%
	ACAGAAGAAAAGTCCGGTGG+TGG	-	chr1.2:77187538-77187557	None:intergenic	50.0%
	ACTTCCCTGAGAAACTACCG+TGG	-	chr1.2:77187968-77187987	None:intergenic	50.0%
	AGTCTTCTTCTTGGTCGTGG+TGG	-	chr1.2:77187492-77187511	None:intergenic	50.0%
	ATCGCCACGGTAGTTTCTCA+GGG	+	chr1.2:77187961-77187980	MS.gene006450:CDS	50.0%
	CGGTAGTTTCTCAGGGAAGT+TGG	+	chr1.2:77187968-77187987	MS.gene006450:CDS	50.0%
	GAGAAACTACCGTGGCGATA+TGG	-	chr1.2:77187960-77187979	None:intergenic	50.0%
	GGAGAATGATGAAGAGGAGG+AGG	-	chr1.2:77187887-77187906	None:intergenic	50.0%
	GGAGGAGAATGATGAAGAGG+AGG	-	chr1.2:77187890-77187909	None:intergenic	50.0%
	TAACCAACGCCATATCGCCA+CGG	+	chr1.2:77187948-77187967	MS.gene006450:CDS	50.0%
	TATCGCCACGGTAGTTTCTC+AGG	+	chr1.2:77187960-77187979	MS.gene006450:CDS	50.0%
!	GTGGTAGTGTGTTGTGGATG+AGG	-	chr1.2:77187412-77187431	None:intergenic	50.0%
!	TGTGATGGAATTGGAGCTGC+CGG	-	chr1.2:77187593-77187612	None:intergenic	50.0%
!	TTTGGAGAAGATAGCGGTGG+CGG	-	chr1.2:77187922-77187941	None:intergenic	50.0%
!!	AGCTTTGGAGAAGATAGCGG+TGG	-	chr1.2:77187925-77187944	None:intergenic	50.0%
	AGGTTCTTCTTCTCCTCCCC+CGG	+	chr1.2:77187571-77187590	MS.gene006450:CDS	55.0%
	CTTCTGTCTTCGCGTCTCAG+AGG	+	chr1.2:77187551-77187570	MS.gene006450:CDS	55.0%
	CTTCTTCTTGGTCGTGGTGG+TGG	-	chr1.2:77187489-77187508	None:intergenic	55.0%
	TGATGGAATTGGAGCTGCCG+GGG	-	chr1.2:77187591-77187610	None:intergenic	55.0%
!	GTGATGGAATTGGAGCTGCC+GGG	-	chr1.2:77187592-77187611	None:intergenic	55.0%
	CTACCGTGGCGATATGGCGT+TGG	-	chr1.2:77187954-77187973	None:intergenic	60.0%
	GATGGAATTGGAGCTGCCGG+GGG	-	chr1.2:77187590-77187609	None:intergenic	60.0%
!!	CTTCTTGGTCGTGGTGGTGG+TGG	-	chr1.2:77187486-77187505	None:intergenic	60.0%
!!	TAGCGGTGGCGGAGATGAAG+AGG	-	chr1.2:77187911-77187930	None:intergenic	60.0%
!!	TTGGTCGTGGTGGTGGTGGT+GGG	-	chr1.2:77187482-77187501	None:intergenic	60.0%
	CACCACGGAGCTTCCACCAC+CGG	+	chr1.2:77187522-77187541	MS.gene006450:CDS	65.0%
!!	CGGTGGCGGAGATGAAGAGG+AGG	-	chr1.2:77187908-77187927	None:intergenic	65.0%
!!	CTTGGTCGTGGTGGTGGTGG+TGG	-	chr1.2:77187483-77187502	None:intergenic	65.0%
!!	GTGGTGGTGGTGGGTGAAGG+TGG	-	chr1.2:77187473-77187492	None:intergenic	65.0%
!!	GTGGTGGTGGTGGTGGGTGA+AGG	-	chr1.2:77187476-77187495	None:intergenic	65.0%
	GGAATTGGAGCTGCCGGGGG+AGG	-	chr1.2:77187587-77187606	None:intergenic	70.0%
!!	GTCCGGTGGTGGAAGCTCCG+TGG	-	chr1.2:77187527-77187546	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	77187337	77188023	77187337	ID=MS.gene006450
chr1.2	mRNA	77187337	77188023	77187337	ID=MS.gene006450.t1;Parent=MS.gene006450
chr1.2	exon	77187337	77188023	77187337	ID=MS.gene006450.t1.exon1;Parent=MS.gene006450.t1
chr1.2	CDS	77187337	77188023	77187337	ID=cds.MS.gene006450.t1;Parent=MS.gene006450.t1

Gene Sequence

>MS.gene006450

ATGCCAAAACTATTCTCTAAACAGCTACACCTCTGTTTCTTCAAGCTCAAATACCCTACAATTCTCTCCCAACCTCATCCACAACACACTACCACTTCATCACCATCATCACCTTGCACTTTCCATTTCAATACCACCTTCACCCACCACCACCACCACGACCAAGAAGAAGACTATTTCTACGACACCACGGAGCTTCCACCACCGGACTTTTCTTCTGTCTTCGCGTCTCAGAGGTTCTTCTTCTCCTCCCCCGGCAGCTCCAATTCCATCACAGAGTCTCCTTCTAACGACACTCTACCTTGCAACTCGTTGATACCAAGAAGAGGTAGTGTGAAGAGGGTGCCAAAATACTCTGTAAACCCTTATGTTGATTTCCTCCGTTCTATGCAGGAAATGATTCAATCTCAACAACAAGTGTTAGATGTTACTAATGATTGGGATTATCTTCATGAACTTCTCTTATGCTATCTTGCTCTTAATCCTACGCATACTCACAAGTATATTGTTCAAGCTTTCACTCACCTTCTTGTTGATATTTTATCTTCCTCCTCCTCTTCATCATTCTCCTCCTCTTCATCTCCGCCACCGCTATCTTCTCCAAAGCTCAATAACCAACGCCATATCGCCACGGTAGTTTCTCAGGGAAGTTGGTGTAAAAATATTAAATGTCAAGGTTTTAAATAG

Protein sequence

>MS.gene006450.t1

MPKLFSKQLHLCFFKLKYPTILSQPHPQHTTTSSPSSPCTFHFNTTFTHHHHHDQEEDYFYDTTELPPPDFSSVFASQRFFFSSPGSSNSITESPSNDTLPCNSLIPRRGSVKRVPKYSVNPYVDFLRSMQEMIQSQQQVLDVTNDWDYLHELLLCYLALNPTHTHKYIVQAFTHLLVDILSSSSSSSFSSSSSPPPLSSPKLNNQRHIATVVSQGSWCKNIKCQGFK