AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00649

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00649.t1 MTR_2g006900 98.333 60 1 0 57 116 1 60 1.07e-40 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGCTGCTGTCTCAATTT+TGG 0.174192 2.1:+75475702 MS.gene00649:CDS
TCGACGAACCATATGTTTCT+TGG 0.274695 2.1:-75477104 None:intergenic
CTTCTTCTTCAACTTCCTAA+TGG 0.279260 2.1:+75477182 MS.gene00649:CDS
TTCTTCTTCAACTTCCTAAT+GGG 0.326811 2.1:+75477183 MS.gene00649:CDS
ACGTCCTCCTCCTCAAAATA+TGG 0.327173 2.1:-75474718 None:intergenic
TATGTGGCGCTCTGGACCTA+TGG 0.393114 2.1:+75475888 MS.gene00649:CDS
GCAATTGGAGCAACATCAAT+TGG 0.393626 2.1:-75475685 None:intergenic
TTCCTAATGGGTTGAGTTGA+GGG 0.396719 2.1:+75477195 MS.gene00649:CDS
GGTTGGACATTGTTTGCAAT+TGG 0.397971 2.1:-75475664 None:intergenic
CGAGATGTCCCTGCTGCCTC+AGG 0.421876 2.1:+75477124 MS.gene00649:CDS
GGATCTCCTCCCATGATTCC+AGG 0.423283 2.1:-75477160 None:intergenic
TGTCCCACCACCTGGAATCA+TGG 0.424311 2.1:+75477150 MS.gene00649:CDS
AAAATTGAGACAGCAGCAAT+TGG 0.438976 2.1:-75475700 None:intergenic
CATTTACTCCAAGAAACATA+TGG 0.446712 2.1:+75477096 MS.gene00649:CDS
AACACTCCTATGTGGCGCTC+TGG 0.449276 2.1:+75475880 MS.gene00649:CDS
TCAAGAAATGCCATTGGGCG+TGG 0.452536 2.1:+75475774 MS.gene00649:CDS
TCAGGTTCTGTCCCACCACC+TGG 0.454678 2.1:+75477142 MS.gene00649:CDS
CCTCCCATGATTCCAGGTGG+TGG 0.456756 2.1:-75477154 None:intergenic
GGCCCTCAACTCAACCCATT+AGG 0.459546 2.1:-75477197 None:intergenic
GGGACAGAACCTGAGGCAGC+AGG 0.482176 2.1:-75477133 None:intergenic
ATGTGGCGCTCTGGACCTAT+GGG 0.485189 2.1:+75475889 MS.gene00649:CDS
GAATTCAGACACTGAAAGTC+AGG 0.494441 2.1:+75474741 MS.gene00649:CDS
TTTCTTGATCGGCACGGACA+TGG 0.499473 2.1:-75475759 None:intergenic
CTTCCTAATGGGTTGAGTTG+AGG 0.500624 2.1:+75477194 MS.gene00649:CDS
GTCCCACCACCTGGAATCAT+GGG 0.504732 2.1:+75477151 MS.gene00649:CDS
CAGAAGGTGGTCGGATCAGA+TGG 0.507419 2.1:-75475832 None:intergenic
AAATTTACCTTTGGACCCAT+AGG 0.510533 2.1:-75475904 None:intergenic
TTGGAGCAACATCAATTGGT+TGG 0.511355 2.1:-75475681 None:intergenic
CTCCCATGATTCCAGGTGGT+GGG 0.519420 2.1:-75477153 None:intergenic
GCCCAATGGCATTTCTTGAT+CGG 0.524113 2.1:-75475770 None:intergenic
GCCGATCAAGAAATGCCATT+GGG 0.532200 2.1:+75475769 MS.gene00649:CDS
CATTAGGAAGTTGAAGAAGA+AGG 0.548858 2.1:-75477181 None:intergenic
TGCCGATCAAGAAATGCCAT+TGG 0.553175 2.1:+75475768 MS.gene00649:CDS
CGACGTCGTCCCACGCCCAA+TGG 0.553398 2.1:-75475784 None:intergenic
CTCTGGACCTATGGGTCCAA+AGG 0.554114 2.1:+75475897 MS.gene00649:CDS
GCAATGAAAATTTGTGCAGA+AGG 0.580559 2.1:-75475848 None:intergenic
CAAGAAATGCCATTGGGCGT+GGG 0.597462 2.1:+75475775 MS.gene00649:CDS
GGACAGAACCTGAGGCAGCA+GGG 0.617193 2.1:-75477132 None:intergenic
AAAGAATCAGTACAATACAT+TGG 0.618095 2.1:-75475736 None:intergenic
ATGGCATTTCTTGATCGGCA+CGG 0.620469 2.1:-75475765 None:intergenic
AAATTTGTGCAGAAGGTGGT+CGG 0.623430 2.1:-75475841 None:intergenic
ATGAAAATTTGTGCAGAAGG+TGG 0.644289 2.1:-75475845 None:intergenic
AGACAAGAAACACTCCTATG+TGG 0.647405 2.1:+75475872 MS.gene00649:CDS
ATAGGTCCAGAGCGCCACAT+AGG 0.654562 2.1:-75475886 None:intergenic
AGGTGGTGGGACAGAACCTG+AGG 0.657762 2.1:-75477140 None:intergenic
CAGATGGACGAGCTCGTCCG+CGG 0.666213 2.1:-75475816 None:intergenic
CCACCACCTGGAATCATGGG+AGG 0.682520 2.1:+75477154 MS.gene00649:CDS
TCTCCTCCCATGATTCCAGG+TGG 0.693154 2.1:-75477157 None:intergenic
CGACGTCGTTACATCAGCCG+CGG 0.703348 2.1:+75475799 MS.gene00649:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAACTTACTTT+AGG - chr2.1:75474802-75474821 None:intergenic 10.0%
!! AAAAAAAAAAACTTACTTTA+GGG - chr2.1:75474801-75474820 None:intergenic 10.0%
!! ATTATTAGATTGTATAAATA+AGG - chr2.1:75476514-75476533 None:intergenic 10.0%
!! TAAGACAAATTAATTTAATT+AGG + chr2.1:75475011-75475030 MS.gene00649:intron 10.0%
!! TTATACAATCTAATAATTAA+AGG + chr2.1:75476517-75476536 MS.gene00649:intron 10.0%
!!! GTCTAATATAATTTTTTTTA+AGG - chr2.1:75474964-75474983 None:intergenic 10.0%
!! AAAAAAAAACACAAAACTAA+CGG - chr2.1:75476361-75476380 None:intergenic 15.0%
!! AAAAATACATCAAATATAAG+TGG - chr2.1:75475116-75475135 None:intergenic 15.0%
!! ATATAAAAAGTAAGGAATTA+TGG + chr2.1:75475997-75476016 MS.gene00649:intron 15.0%
!! CATCTTATATATAAAAAGTA+AGG + chr2.1:75475989-75476008 MS.gene00649:intron 15.0%
!! TTAAAAAAACACAAAACTAT+TGG + chr2.1:75476230-75476249 MS.gene00649:intron 15.0%
!!! ATTTTTGAGACATTATTTAA+AGG + chr2.1:75476081-75476100 MS.gene00649:intron 15.0%
!!! TTTTTTAACTAATAATCAGT+TGG - chr2.1:75475936-75475955 None:intergenic 15.0%
!! CTTTGTAAAAATATTGGTAT+TGG + chr2.1:75476188-75476207 MS.gene00649:intron 20.0%
!! CTTTGTAAAAATATTGGTAT+TGG - chr2.1:75476207-75476188 None:intergenic 20.0%
!! CTTTGTAAAAATATTGTCAT+TGG - chr2.1:75476859-75476878 None:intergenic 20.0%
!! TAAAGGTTTAAATGTGTAAT+TGG + chr2.1:75476534-75476553 MS.gene00649:intron 20.0%
!!! CAATATTTTTACAAAGTACA+GGG - chr2.1:75476263-75476282 None:intergenic 20.0%
! AAACACAAAACTATTGGTAT+TGG + chr2.1:75476236-75476255 MS.gene00649:intron 25.0%
! AAAGAATCAGTACAATACAT+TGG - chr2.1:75475739-75475758 None:intergenic 25.0%
! AATTAGATGTTTCTTGACTT+TGG + chr2.1:75475324-75475343 MS.gene00649:intron 25.0%
! ATAAGGTTTAAATGTGTCAT+TGG - chr2.1:75476497-75476516 None:intergenic 25.0%
! ATTCCAAACAAAAAAAATGC+AGG - chr2.1:75476157-75476176 None:intergenic 25.0%
! CCTGTACTTTGTAAAAATAT+TGG + chr2.1:75476261-75476280 MS.gene00649:intron 25.0%
! CTTTGTAAAAATATTGGCAT+TGG + chr2.1:75476625-75476644 MS.gene00649:intron 25.0%
! CTTTGTAAAAATATTGGTAG+TGG + chr2.1:75476298-75476317 MS.gene00649:intron 25.0%
! CTTTGTGAAAATATTGGTAT+TGG - chr2.1:75476406-75476425 None:intergenic 25.0%
! TCTCAAAAATTCAACTAAGT+TGG - chr2.1:75476071-75476090 None:intergenic 25.0%
! TTATGGTTTAAATGTGTAAC+TGG - chr2.1:75476950-75476969 None:intergenic 25.0%
! TTCCAAACAAAAAAAATGCA+GGG - chr2.1:75476156-75476175 None:intergenic 25.0%
! TTTAACAGAAAGTTAACAGA+GGG + chr2.1:75476379-75476398 MS.gene00649:intron 25.0%
! TTTAACAGAAAGTTAACAGA+GGG - chr2.1:75476398-75476379 None:intergenic 25.0%
!! CAAAATTTTTACAAAGTGCA+GGG + chr2.1:75476772-75476791 MS.gene00649:intron 25.0%
!! TTTTAACAGAAAGTTAACAG+AGG + chr2.1:75476378-75476397 MS.gene00649:intron 25.0%
!! TTTTAACAGAAAGTTAACAG+AGG - chr2.1:75476397-75476378 None:intergenic 25.0%
!!! AAAAATTCAACTAAGTTGGT+GGG - chr2.1:75476067-75476086 None:intergenic 25.0%
!!! ACAATATTTTTACAAAGTGC+AGG + chr2.1:75476862-75476881 MS.gene00649:intron 25.0%
!!! AGACTCAAAAGTTTTGATTA+GGG - chr2.1:75475169-75475188 None:intergenic 25.0%
!!! CAATATTTTTACAAAGTGCA+GGG + chr2.1:75476803-75476822 MS.gene00649:intron 25.0%
!!! CAATATTTTTACAAAGTGCA+GGG - chr2.1:75476822-75476803 None:intergenic 25.0%
!!! CACTTTTTTTTGTTTGACAT+TGG + chr2.1:75476594-75476613 MS.gene00649:intron 25.0%
!!! CACTTTTTTTTGTTTGACAT+TGG - chr2.1:75476613-75476594 None:intergenic 25.0%
!!! CCAATATTTTTACAAAGTAC+AGG - chr2.1:75476264-75476283 None:intergenic 25.0%
!!! GCATTTTTTTTGTTTGGAAT+TGG + chr2.1:75476157-75476176 MS.gene00649:intron 25.0%
!!! TAGACTCAAAAGTTTTGATT+AGG - chr2.1:75475170-75475189 None:intergenic 25.0%
AAACGTCAGTGACTTAATTA+CGG + chr2.1:75475491-75475510 MS.gene00649:intron 30.0%
AGTCTAATGCACCTAATAAA+TGG + chr2.1:75475445-75475464 MS.gene00649:intron 30.0%
CAAATTTCCCACCATTTATT+AGG - chr2.1:75475459-75475478 None:intergenic 30.0%
CATTTACTCCAAGAAACATA+TGG + chr2.1:75477096-75477115 MS.gene00649:CDS 30.0%
CCTGCACTTTGTAAAAATAT+TGG + chr2.1:75476182-75476201 MS.gene00649:intron 30.0%
CCTGCACTTTGTAAAAATAT+TGG - chr2.1:75476201-75476182 None:intergenic 30.0%
CTAAGAGATGAAAATTGCAT+TGG + chr2.1:75474914-75474933 MS.gene00649:intron 30.0%
CTTTGGACAAATATTGGTAT+TGG + chr2.1:75476716-75476735 MS.gene00649:intron 30.0%
TAATGCACCTAATAAATGGT+GGG + chr2.1:75475449-75475468 MS.gene00649:intron 30.0%
TTCTTCTTCAACTTCCTAAT+GGG + chr2.1:75477183-75477202 MS.gene00649:CDS 30.0%
TTTATTAGGTGCATTAGACT+TGG - chr2.1:75475445-75475464 None:intergenic 30.0%
! AATAAATGGTGGGAAATTTG+AGG + chr2.1:75475459-75475478 MS.gene00649:intron 30.0%
! CAAAAATTCAACTAAGTTGG+TGG - chr2.1:75476068-75476087 None:intergenic 30.0%
! CAATATTTTCACAAAGTGCA+GGG + chr2.1:75476410-75476429 MS.gene00649:intron 30.0%
! CCAAAATTTTTACAAAGTGC+AGG + chr2.1:75476771-75476790 MS.gene00649:intron 30.0%
! CCTGCACTTTGTAAAAATTT+TGG - chr2.1:75476774-75476793 None:intergenic 30.0%
! GTCAAACAAAAAAAAGTGCA+GGG + chr2.1:75476832-75476851 MS.gene00649:intron 30.0%
! GTCAAACAAAAAAAAGTGCA+GGG - chr2.1:75476851-75476832 None:intergenic 30.0%
! TGTCAAACAAAAAAAAGTGC+AGG + chr2.1:75476831-75476850 MS.gene00649:intron 30.0%
! TGTCAAACAAAAAAAAGTGC+AGG - chr2.1:75476850-75476831 None:intergenic 30.0%
!! CCAATATTTTTACAAAGTGC+AGG + chr2.1:75476802-75476821 MS.gene00649:intron 30.0%
!! CCAATATTTTTACAAAGTGC+AGG - chr2.1:75476821-75476802 None:intergenic 30.0%
!! TGGTGTTGTAAATTTACCTT+TGG - chr2.1:75475916-75475935 None:intergenic 30.0%
!!! ACTCAAAAGTTTTGATTAGG+GGG - chr2.1:75475167-75475186 None:intergenic 30.0%
!!! CACTTTTTTTTGTTTGGCAT+TGG - chr2.1:75476890-75476909 None:intergenic 30.0%
!!! GACTCAAAAGTTTTGATTAG+GGG - chr2.1:75475168-75475187 None:intergenic 30.0%
!!! GTTAGTTTTGAGTGTATTTG+AGG - chr2.1:75475626-75475645 None:intergenic 30.0%
AAAATTGAGACAGCAGCAAT+TGG - chr2.1:75475703-75475722 None:intergenic 35.0%
AAATTTACCTTTGGACCCAT+AGG - chr2.1:75475907-75475926 None:intergenic 35.0%
AACTAACAACGTTTGTGAAC+AGG + chr2.1:75475639-75475658 MS.gene00649:intron 35.0%
ATGAAAATTTGTGCAGAAGG+TGG - chr2.1:75475848-75475867 None:intergenic 35.0%
CAATATTTGTCCAAAGTGCA+GGG - chr2.1:75476712-75476731 None:intergenic 35.0%
CATTAGGAAGTTGAAGAAGA+AGG - chr2.1:75477184-75477203 None:intergenic 35.0%
CCTGCACTTTGTGAAAATAT+TGG - chr2.1:75476412-75476431 None:intergenic 35.0%
CTAATGCACCTAATAAATGG+TGG + chr2.1:75475448-75475467 MS.gene00649:intron 35.0%
CTTCTTCTTCAACTTCCTAA+TGG + chr2.1:75477182-75477201 MS.gene00649:CDS 35.0%
GCAATGAAAATTTGTGCAGA+AGG - chr2.1:75475851-75475870 None:intergenic 35.0%
GTAATTAAGTCACTGACGTT+TGG - chr2.1:75475492-75475511 None:intergenic 35.0%
TATCTCTCGACATGTGATTT+GGG + chr2.1:75475237-75475256 MS.gene00649:intron 35.0%
TTATCTCTCGACATGTGATT+TGG + chr2.1:75475236-75475255 MS.gene00649:intron 35.0%
! AATTGCTGCTGTCTCAATTT+TGG + chr2.1:75475702-75475721 MS.gene00649:CDS 35.0%
! CAAAATCAGTTGAAAGTGCA+AGG + chr2.1:75476923-75476942 MS.gene00649:intron 35.0%
! CAAAATCTGATGAAAGTGCA+GGG + chr2.1:75476470-75476489 MS.gene00649:intron 35.0%
! CAAAATCTGATGAAAGTGCA+GGG - chr2.1:75476489-75476470 None:intergenic 35.0%
! CAAAATCTGTTGAAAGTGCA+GGG - chr2.1:75476561-75476580 None:intergenic 35.0%
! CCAATATTTTCACAAAGTGC+AGG + chr2.1:75476409-75476428 MS.gene00649:intron 35.0%
! CTAGCACTCACTCTTTTATT+TGG + chr2.1:75475399-75475418 MS.gene00649:intron 35.0%
! GCCAAACAAAAAAAAGTGCA+GGG + chr2.1:75476892-75476911 MS.gene00649:intron 35.0%
! TGCCAAACAAAAAAAAGTGC+AGG + chr2.1:75476891-75476910 MS.gene00649:intron 35.0%
!! CTTGCACTTTCAACTGATTT+TGG - chr2.1:75476925-75476944 None:intergenic 35.0%
!! CTTTCAACAGATTTTGGCAT+TGG + chr2.1:75476565-75476584 MS.gene00649:intron 35.0%
!! CTTTCAACTGATTTTGGCAT+TGG - chr2.1:75476919-75476938 None:intergenic 35.0%
!! CTTTCATCAGATTTTGGCAT+TGG + chr2.1:75476129-75476148 MS.gene00649:intron 35.0%
!! CTTTCATCAGATTTTGGCAT+TGG - chr2.1:75476148-75476129 None:intergenic 35.0%
!!! CACTCTTTTTTGTTTGGCAT+TGG - chr2.1:75476437-75476456 None:intergenic 35.0%
!!! CACTTTTTTTTGTCTGGCAT+TGG + chr2.1:75476654-75476673 MS.gene00649:intron 35.0%
!!! CTCAAAAGTTTTGATTAGGG+GGG - chr2.1:75475166-75475185 None:intergenic 35.0%
!!! GTCCCTGCATTTTTTTTGTT+TGG + chr2.1:75476151-75476170 MS.gene00649:intron 35.0%
!!! TCCCTGCACTTTTTTTTGTT+TGG - chr2.1:75476607-75476588 None:intergenic 35.0%
AAATTTGTGCAGAAGGTGGT+CGG - chr2.1:75475844-75475863 None:intergenic 40.0%
AGACAAGAAACACTCCTATG+TGG + chr2.1:75475872-75475891 MS.gene00649:CDS 40.0%
ATCTCTCGACATGTGATTTG+GGG + chr2.1:75475238-75475257 MS.gene00649:intron 40.0%
ATTAGGTGCATTAGACTTGG+TGG - chr2.1:75475442-75475461 None:intergenic 40.0%
CCAATATTTGTCCAAAGTGC+AGG - chr2.1:75476713-75476732 None:intergenic 40.0%
CCATGTGGCATGAAATGATT+GGG + chr2.1:75476323-75476342 MS.gene00649:intron 40.0%
CCTGCACTTTGGACAAATAT+TGG + chr2.1:75476710-75476729 MS.gene00649:intron 40.0%
GAATTCAGACACTGAAAGTC+AGG + chr2.1:75474741-75474760 MS.gene00649:CDS 40.0%
GCAATTGGAGCAACATCAAT+TGG - chr2.1:75475688-75475707 None:intergenic 40.0%
GGTTGGACATTGTTTGCAAT+TGG - chr2.1:75475667-75475686 None:intergenic 40.0%
TCGACGAACCATATGTTTCT+TGG - chr2.1:75477107-75477126 None:intergenic 40.0%
TTAAGTCACTGACGTTTGGT+AGG - chr2.1:75475488-75475507 None:intergenic 40.0%
TTGGAGCAACATCAATTGGT+TGG - chr2.1:75475684-75475703 None:intergenic 40.0%
! CCAAAATCTGATGAAAGTGC+AGG + chr2.1:75476469-75476488 MS.gene00649:intron 40.0%
! CCAAAATCTGATGAAAGTGC+AGG - chr2.1:75476488-75476469 None:intergenic 40.0%
! CCAAAATCTGTTGAAAGTGC+AGG - chr2.1:75476562-75476581 None:intergenic 40.0%
! CCTGCACTTTCAACAGATTT+TGG + chr2.1:75476559-75476578 MS.gene00649:intron 40.0%
! CCTGCACTTTCATCAGATTT+TGG + chr2.1:75476123-75476142 MS.gene00649:intron 40.0%
! CCTGCACTTTCATCAGATTT+TGG - chr2.1:75476142-75476123 None:intergenic 40.0%
! GCCAAACAAAAAAGAGTGCA+GGG + chr2.1:75476439-75476458 MS.gene00649:intron 40.0%
! GCCAGACAAAAAAAAGTGCA+GGG - chr2.1:75476652-75476671 None:intergenic 40.0%
! GGCTAACTGCATGAACATTT+TGG - chr2.1:75476046-75476065 None:intergenic 40.0%
! TGCCAAACAAAAAAGAGTGC+AGG + chr2.1:75476438-75476457 MS.gene00649:intron 40.0%
! TGCCAGACAAAAAAAAGTGC+AGG - chr2.1:75476653-75476672 None:intergenic 40.0%
! TTAGGTGCATTAGACTTGGT+GGG - chr2.1:75475441-75475460 None:intergenic 40.0%
!! GCATGAACATTTTGGTATGC+TGG - chr2.1:75476038-75476057 None:intergenic 40.0%
!!! TCCCTGCACTCTTTTTTGTT+TGG - chr2.1:75476443-75476462 None:intergenic 40.0%
!!! TCCCTGCACTTTTTTTTGTC+TGG + chr2.1:75476648-75476667 MS.gene00649:intron 40.0%
ACGTCCTCCTCCTCAAAATA+TGG - chr2.1:75474721-75474740 None:intergenic 45.0%
ATGGCATTTCTTGATCGGCA+CGG - chr2.1:75475768-75475787 None:intergenic 45.0%
CATGAAATGATTGGGCCATG+TGG + chr2.1:75476331-75476350 MS.gene00649:intron 45.0%
CCACGTGGCATGAAATGATT+GGG + chr2.1:75476741-75476760 MS.gene00649:intron 45.0%
CCCAATCATTTCATGCCACA+TGG - chr2.1:75476326-75476345 None:intergenic 45.0%
GCCATGTGGCATGAAATGAT+TGG + chr2.1:75476322-75476341 MS.gene00649:intron 45.0%
GCCCAATGGCATTTCTTGAT+CGG - chr2.1:75475773-75475792 None:intergenic 45.0%
GCCGATCAAGAAATGCCATT+GGG + chr2.1:75475769-75475788 MS.gene00649:CDS 45.0%
TGCCGATCAAGAAATGCCAT+TGG + chr2.1:75475768-75475787 MS.gene00649:CDS 45.0%
! AAAACTAACGGAGTGCCACA+TGG - chr2.1:75476349-75476368 None:intergenic 45.0%
! GACTTTTTGTGCAATGCGTG+TGG + chr2.1:75477073-75477092 MS.gene00649:intron 45.0%
!! ATATTGGTAGTGGTGCCATG+TGG + chr2.1:75476308-75476327 MS.gene00649:intron 45.0%
!! ATATTGGTATTGGTGCCACG+TGG + chr2.1:75476726-75476745 MS.gene00649:intron 45.0%
!! AAAAAATTTAAGAAAAATAA+AGG - chr2.1:75475269-75475288 None:intergenic 5.0%
!! AATTAAATAAAAAAGAAATT+AGG + chr2.1:75476968-75476987 MS.gene00649:intron 5.0%
!!! ATTTCTTTTTTATTTAATTA+TGG - chr2.1:75476967-75476986 None:intergenic 5.0%
!!! TTTTTATTGAAATAATAAAA+TGG + chr2.1:75475595-75475614 MS.gene00649:intron 5.0%
CAAGAAATGCCATTGGGCGT+GGG + chr2.1:75475775-75475794 MS.gene00649:CDS 50.0%
CCCAATCATTTCATGCCACG+TGG - chr2.1:75476744-75476763 None:intergenic 50.0%
GCCACGTGGCATGAAATGAT+TGG + chr2.1:75476740-75476759 MS.gene00649:intron 50.0%
TCAAGAAATGCCATTGGGCG+TGG + chr2.1:75475774-75475793 MS.gene00649:CDS 50.0%
TTTCTTGATCGGCACGGACA+TGG - chr2.1:75475762-75475781 None:intergenic 50.0%
!! TGGTATTGGTCCCTGCACTT+TGG + chr2.1:75476281-75476300 MS.gene00649:intron 50.0%
AACACTCCTATGTGGCGCTC+TGG + chr2.1:75475880-75475899 MS.gene00649:CDS 55.0%
CAGAAGGTGGTCGGATCAGA+TGG - chr2.1:75475835-75475854 None:intergenic 55.0%
CTCCCATGATTCCAGGTGGT+GGG - chr2.1:75477156-75477175 None:intergenic 55.0%
CTCTGGACCTATGGGTCCAA+AGG + chr2.1:75475897-75475916 MS.gene00649:CDS 55.0%
GGATCTCCTCCCATGATTCC+AGG - chr2.1:75477163-75477182 None:intergenic 55.0%
GTCCCACCACCTGGAATCAT+GGG + chr2.1:75477151-75477170 MS.gene00649:CDS 55.0%
TCTCCTCCCATGATTCCAGG+TGG - chr2.1:75477160-75477179 None:intergenic 55.0%
TGTCCCACCACCTGGAATCA+TGG + chr2.1:75477150-75477169 MS.gene00649:CDS 55.0%
! ATAGGTCCAGAGCGCCACAT+AGG - chr2.1:75475889-75475908 None:intergenic 55.0%
! ATGTGGCGCTCTGGACCTAT+GGG + chr2.1:75475889-75475908 MS.gene00649:CDS 55.0%
! TATGTGGCGCTCTGGACCTA+TGG + chr2.1:75475888-75475907 MS.gene00649:CDS 55.0%
CCACCACCTGGAATCATGGG+AGG + chr2.1:75477154-75477173 MS.gene00649:CDS 60.0%
CCTCCCATGATTCCAGGTGG+TGG - chr2.1:75477157-75477176 None:intergenic 60.0%
CGACGTCGTTACATCAGCCG+CGG + chr2.1:75475799-75475818 MS.gene00649:CDS 60.0%
GGACAGAACCTGAGGCAGCA+GGG - chr2.1:75477135-75477154 None:intergenic 60.0%
TCAGGTTCTGTCCCACCACC+TGG + chr2.1:75477142-75477161 MS.gene00649:CDS 60.0%
!! AGGTGGTGGGACAGAACCTG+AGG - chr2.1:75477143-75477162 None:intergenic 60.0%
CAGATGGACGAGCTCGTCCG+CGG - chr2.1:75475819-75475838 None:intergenic 65.0%
CGAGATGTCCCTGCTGCCTC+AGG + chr2.1:75477124-75477143 MS.gene00649:CDS 65.0%
GGGACAGAACCTGAGGCAGC+AGG - chr2.1:75477136-75477155 None:intergenic 65.0%
CGACGTCGTCCCACGCCCAA+TGG - chr2.1:75475787-75475806 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 75474715 75477214 75474715 ID=MS.gene00649
chr2.1 mRNA 75474715 75477214 75474715 ID=MS.gene00649.t1;Parent=MS.gene00649
chr2.1 exon 75474715 75474804 75474715 ID=MS.gene00649.t1.exon1;Parent=MS.gene00649.t1
chr2.1 CDS 75474715 75474804 75474715 ID=cds.MS.gene00649.t1;Parent=MS.gene00649.t1
chr2.1 exon 75475661 75475918 75475661 ID=MS.gene00649.t1.exon2;Parent=MS.gene00649.t1
chr2.1 CDS 75475661 75475918 75475661 ID=cds.MS.gene00649.t1;Parent=MS.gene00649.t1
chr2.1 exon 75477074 75477214 75477074 ID=MS.gene00649.t1.exon3;Parent=MS.gene00649.t1
chr2.1 CDS 75477074 75477214 75477074 ID=cds.MS.gene00649.t1;Parent=MS.gene00649.t1
Gene Sequence

>MS.gene00649

ATGCCATATTTTGAGGAGGAGGACGTGAATTCAGACACTGAAAGTCAGGTTTCATCACACATTCAGAGCAATACTGAAAATATCCCTAAAGTAAGTTTTTTTTTTTTTTTTCTCTTTCTATAATAATGAATGTCACATACATAATAGTAGTACATATATGACTGTTAAAAGAAGTTAACATTGTCGATTTTTGTATCATCTAAGAGATGAAAATTGCATTGGCTGTTAAAAAAACTAGAATTTTTTCCTTAAAAAAAATTATATTAGACTTAGTTCATTTGTATAAAATTGACTTGTAAGACAAATTAATTTAATTAGGATATTTTGTTATTATGCAATTATTTTATACGTGAATCTAATGATGTGTTGTCACTTTTTTTATATAAAATTATTATTTACCACTTATATTTGATGTATTTTTATAAATATTCATAGCATATAAGTATGTCCCCCCTAATCAAAACTTTTGAGTCTAACTTTTAAGATGTTGTGTGTCACTCTTTTATGAATTTTACTATAATTTATCTCTCGACATGTGATTTGGGGATTTTCCTTTATTTTTCTTAAATTTTTTATGTGATGATGCAATTTAATCATCATGTGAATGAAAATTAGATGTTTCTTGACTTTGGTATTTAGTCGCATGAGCAATGCTAGAAACACTCTCTTTTGAGCACTCTCTCTCTAGCACTCACTCTTTTATTTGGTGAAATCAATGTATGTCCCACCAAGTCTAATGCACCTAATAAATGGTGGGAAATTTGAGGATGCCTACCAAACGTCAGTGACTTAATTACGGTAGAATAATTAACATACAAAAAGTGAACAACTAGTGTTATTATGTGATTCTAAATAATTAAATAAAATGATTGTGTATTTATTTTTATTGAAATAATAAAATGGTGACACCTCAAATACACTCAAAACTAACAACGTTTGTGAACAGGATCCAATTGCAAACAATGTCCAACCAATTGATGTTGCTCCAATTGCTGCTGTCTCAATTTTGGTTGATGCTCAACCAATGTATTGTACTGATTCTTTCCATGTCCGTGCCGATCAAGAAATGCCATTGGGCGTGGGACGACGTCGTTACATCAGCCGCGGACGAGCTCGTCCATCTGATCCGACCACCTTCTGCACAAATTTTCATTGCAAGACAAGAAACACTCCTATGTGGCGCTCTGGACCTATGGGTCCAAAGGTAAATTTACAACACCAACTGATTATTAGTTAAAAAAATTATGTCAAATAGTCTAGTGTCGAGAATAACACATCTTATATATAAAAAGTAAGGAATTATGGATTGAAACTCACGCTCCAGCATACCAAAATGTTCATGCAGTTAGCCCACCAACTTAGTTGAATTTTTGAGACATTATTTAAAGGCTTAAATATGTCTTTAGTCCCTGCACTTTCATCAGATTTTGGCATTGGTCCCTGCATTTTTTTTGTTTGGAATTGGTCCCTGCACTTTGTAAAAATATTGGTATTGGTCCCTCTGTTAACTTTCTGTTAAAAAAACACAAAACTATTGGTATTGGTCCCTGTACTTTGTAAAAATATTGGTATTGGTCCCTGCACTTTGTAAAAATATTGGTAGTGGTGCCATGTGGCATGAAATGATTGGGCCATGTGGCACTCCGTTAGTTTTGTGTTTTTTTTTTAACAGAAAGTTAACAGAGGGACCAATACCAATATTTTCACAAAGTGCAGGGACCAATGCCAAACAAAAAAGAGTGCAGGGACCAATGCCAAAATCTGATGAAAGTGCAGGGACCAATGACACATTTAAACCTTATTTATACAATCTAATAATTAAAGGTTTAAATGTGTAATTGGTCCCTGCACTTTCAACAGATTTTGGCATTGGTCCCTGCACTTTTTTTTGTTTGACATTGGTCCCTGCACTTTGTAAAAATATTGGCATTGGTCCCTGCACTTTTTTTTGTCTGGCATTGGTCCCTGCACTTTGTAAAAATATTGGTATTGGTCCCTGCACTTTGGACAAATATTGGTATTGGTGCCACGTGGCATGAAATGATTGGGATCAATACCAAAATTTTTACAAAGTGCAGGGACCAATACCAATATTTTTACAAAGTGCAGGGACCAATGTCAAACAAAAAAAAGTGCAGGGACCAATGACAATATTTTTACAAAGTGCAGGGACCAATGCCAAACAAAAAAAAGTGCAGGGACCAATGCCAAAATCAGTTGAAAGTGCAAGGACCAGTTACACATTTAAACCATAATTAAATAAAAAAGAAATTAGGAAACACACTATTTATTAATAATAGTAATATTATTAACTATTTTACGATCGTCATTATTCTTCTGTGTTATATACTATGTGCAGACTTTTTGTGCAATGCGTGTGGCATTTACTCCAAGAAACATATGGTTCGTCGAGATGTCCCTGCTGCCTCAGGTTCTGTCCCACCACCTGGAATCATGGGAGGAGATCCTTCTTCTTCAACTTCCTAATGGGTTGAGTTGA
Protein sequence

>MS.gene00649.t1

MPYFEEEDVNSDTESQVSSHIQSNTENIPKDPIANNVQPIDVAPIAAVSILVDAQPMYCTDSFHVRADQEMPLGVGRRRYISRGRARPSDPTTFCTNFHCKTRNTPMWRSGPMGPKTFCAMRVAFTPRNIWFVEMSLLPQVLSHHLESWEEILLLQLPNGLS