AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006572

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006572.t1 MTR_4g077970 98.174 493 9 0 1 493 1 493 0.0 1006
MS.gene006572.t1 MTR_4g077970 97.977 346 7 0 148 493 1 346 0.0 694
MS.gene006572.t1 MTR_5g029680 46.800 500 214 17 11 489 31 499 8.41e-128 381
MS.gene006572.t1 MTR_3g104550 54.260 223 90 3 11 233 45 255 3.83e-74 239
MS.gene006572.t1 MTR_7g095680 56.522 207 87 1 11 217 48 251 4.68e-74 238
MS.gene006572.t1 MTR_1g102860 55.714 210 82 4 11 217 39 240 1.54e-69 225
MS.gene006572.t1 MTR_1g061170 41.254 303 151 6 10 309 47 325 2.54e-69 229
MS.gene006572.t1 MTR_1g071360 51.643 213 83 3 12 221 32 227 2.96e-63 209
MS.gene006572.t1 MTR_1g071360 51.174 213 84 3 12 221 29 224 5.38e-63 209
MS.gene006572.t1 MTR_8g105780 42.537 268 146 2 12 278 22 282 7.99e-63 209
MS.gene006572.t1 MTR_2g100680 43.602 211 108 2 12 222 31 230 2.72e-54 189
MS.gene006572.t1 MTR_8g083100 45.498 211 103 3 13 223 23 221 5.54e-54 189
MS.gene006572.t1 MTR_6g043060 42.986 221 114 3 2 222 12 220 6.03e-54 184
MS.gene006572.t1 MTR_8g087540 44.865 185 94 2 10 193 10 187 5.63e-52 181
MS.gene006572.t1 MTR_2g100670 46.486 185 89 1 12 196 33 207 3.54e-51 176
MS.gene006572.t1 MTR_6g086805 44.811 212 105 6 7 218 6 205 1.02e-50 176
MS.gene006572.t1 MTR_2g100670 46.486 185 89 1 12 196 141 315 3.63e-50 177
MS.gene006572.t1 MTR_2g017890 39.683 189 103 2 10 194 13 194 1.73e-44 160
MS.gene006572.t1 MTR_5g010680 65.217 92 32 0 12 103 24 115 1.20e-39 147
MS.gene006572.t1 MTR_3g101870 42.640 197 64 4 9 156 21 217 2.13e-39 147
MS.gene006572.t1 MTR_0020s0020 65.217 92 32 0 12 103 33 124 9.15e-38 141
MS.gene006572.t1 MTR_1g061170 35.685 241 128 6 72 309 14 230 4.29e-37 140
MS.gene006572.t1 MTR_5g017470 62.366 93 35 0 12 104 9 101 8.16e-37 137
MS.gene006572.t1 MTR_1g100777 60.870 92 36 0 12 103 18 109 6.80e-36 135
MS.gene006572.t1 MTR_5g089170 61.290 93 36 0 12 104 25 117 2.49e-34 130
MS.gene006572.t1 MTR_7g091370 42.484 153 77 2 12 157 22 170 2.83e-31 120
MS.gene006572.t1 MTR_4g088900 54.639 97 40 1 12 104 33 129 1.55e-30 118
MS.gene006572.t1 MTR_5g047190 48.936 94 41 2 20 106 1 94 1.85e-21 89.4
MS.gene006572.t1 MTR_6g044340 28.910 211 129 6 58 267 8 198 4.08e-18 84.7
MS.gene006572.t1 MTR_4g045757 58.333 60 25 0 10 69 12 71 9.69e-18 78.2
MS.gene006572.t1 MTR_2g043940 32.593 135 70 3 69 193 17 140 4.04e-14 74.3
MS.gene006572.t1 MTR_1g061170 30.909 165 95 4 148 309 1 149 1.72e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006572.t1 AT5G16820 48.491 497 196 11 10 489 25 478 1.62e-136 403
MS.gene006572.t1 AT5G16820 48.491 497 196 11 10 489 25 478 1.62e-136 403
MS.gene006572.t1 AT3G02990 48.441 481 211 9 10 489 21 465 1.97e-131 389
MS.gene006572.t1 AT1G32330 44.177 498 219 14 3 489 28 477 2.10e-116 351
MS.gene006572.t1 AT1G32330 44.177 498 219 14 3 489 28 477 3.08e-116 351
MS.gene006572.t1 AT4G17750 43.340 503 211 16 6 489 46 493 4.97e-111 338
MS.gene006572.t1 AT2G26150 58.095 210 80 2 11 218 43 246 1.78e-80 254
MS.gene006572.t1 AT2G26150 58.095 210 80 2 11 218 43 246 1.78e-80 254
MS.gene006572.t1 AT3G22830 56.731 208 89 1 11 217 60 267 3.73e-74 240
MS.gene006572.t1 AT1G67970 44.366 284 131 8 10 286 17 280 8.49e-64 212
MS.gene006572.t1 AT5G03720 44.351 239 112 5 10 243 53 275 5.26e-61 206
MS.gene006572.t1 AT5G03720 44.351 239 112 5 10 243 53 275 5.26e-61 206
MS.gene006572.t1 AT3G51910 44.700 217 95 3 4 216 21 216 1.66e-57 192
MS.gene006572.t1 AT4G13980 44.928 207 101 3 13 218 24 218 5.86e-56 194
MS.gene006572.t1 AT3G24520 42.925 212 90 4 9 218 14 196 4.30e-52 179
MS.gene006572.t1 AT5G43840 39.834 241 102 6 11 244 16 220 1.85e-50 174
MS.gene006572.t1 AT4G18880 37.134 307 172 6 4 308 7 294 2.09e-48 172
MS.gene006572.t1 AT5G54070 41.538 195 99 1 9 203 68 247 5.88e-48 169
MS.gene006572.t1 AT3G63350 44.103 195 97 2 12 205 28 211 2.15e-47 166
MS.gene006572.t1 AT2G26150 56.376 149 57 2 72 218 49 191 4.36e-47 165
MS.gene006572.t1 AT2G26150 56.376 149 57 2 72 218 49 191 4.36e-47 165
MS.gene006572.t1 AT5G45710 40.889 225 111 5 10 232 11 215 7.68e-47 166
MS.gene006572.t1 AT5G45710 40.889 225 111 5 10 232 11 215 7.68e-47 166
MS.gene006572.t1 AT1G46264 41.624 197 74 3 1 156 23 219 9.57e-42 152
MS.gene006572.t1 AT5G62020 61.702 94 36 0 12 105 23 116 5.38e-37 138
MS.gene006572.t1 AT4G36990 52.672 131 48 2 1 129 1 119 1.25e-36 137
MS.gene006572.t1 AT4G11660 59.783 92 37 0 12 103 59 150 1.05e-34 134
MS.gene006572.t1 AT2G41690 55.000 100 45 0 11 110 39 138 7.66e-33 125
MS.gene006572.t1 AT4G18870 39.773 88 53 0 12 99 146 233 7.61e-19 87.4
MS.gene006572.t1 AT4G18870 43.820 89 45 2 13 99 14 99 1.61e-18 86.3
MS.gene006572.t1 AT1G77570 37.778 90 53 2 13 99 27 116 2.11e-15 73.9

Find 112 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAGGTTCCACCTATTTC+TGG 0.099338 4.4:+32580225 MS.gene006572:CDS
AGTTCATCCACCCAAGTTTC+TGG 0.190167 4.4:+32580192 MS.gene006572:CDS
AGCACACCAAGACAATATTT+TGG 0.232538 4.4:+32580377 MS.gene006572:CDS
CAGGAATCAACGATGAATTC+TGG 0.282845 4.4:+32580580 MS.gene006572:CDS
TTTAGGCCAAGCCCACTTAC+TGG 0.290833 4.4:+32580612 MS.gene006572:CDS
TGGTGTGCTGTTGTAAATTC+TGG 0.291113 4.4:-32580363 None:intergenic
GGATGTGATATCAGCTGTTT+TGG 0.314761 4.4:+32580494 MS.gene006572:CDS
TTGTTGTGTTTGAAATATTT+AGG 0.323777 4.4:-32578778 None:intergenic
AAAGGTAGTTCTCTAGGATA+TGG 0.328048 4.4:+32580651 MS.gene006572:CDS
AAGAGGATTATGTGGATTCT+TGG 0.333992 4.4:-32580004 None:intergenic
CAACCGAATCGATCGTTTCA+TGG 0.334490 4.4:+32578695 MS.gene006572:CDS
TTTGGATGGGACACGGTCTT+TGG 0.334831 4.4:+32580512 MS.gene006572:CDS
ATACATGACTGCCCAGAAAT+AGG 0.338814 4.4:-32580237 None:intergenic
AACTGTTCATTTGCAACATC+TGG 0.343307 4.4:-32580086 None:intergenic
CTATTGGTCATACAACTTAC+AGG 0.350342 4.4:-32580279 None:intergenic
TGTGTGGAAGTGGGTAAGTT+TGG 0.353975 4.4:+32579694 MS.gene006572:CDS
CTGAAGGAACATCATCAATA+AGG 0.355963 4.4:-32580149 None:intergenic
TCTTTGCTAATGACAATTCC+TGG 0.358563 4.4:-32580686 None:intergenic
CTGAGGTTCCACCTATTTCT+GGG 0.367826 4.4:+32580226 MS.gene006572:CDS
TTTGCAGCCTCATTAATAGA+AGG 0.376695 4.4:-32580051 None:intergenic
CATGAATAGTCCGGGCTTCA+TGG 0.377627 4.4:+32579888 MS.gene006572:CDS
CAGAATTCATCGTTGATTCC+TGG 0.379940 4.4:-32580579 None:intergenic
GGAGACACAGACGAAGTTAA+AGG 0.386040 4.4:+32580633 MS.gene006572:CDS
AATCCACATAATCCTCTTGA+TGG 0.386726 4.4:+32580009 MS.gene006572:CDS
CCTAAGCAAAACCTACGATA+TGG 0.391487 4.4:+32578660 MS.gene006572:CDS
ATATTTAGGCAAGATGTGTT+TGG 0.394875 4.4:-32578764 None:intergenic
GTGTCTCCAGTAAGTGGGCT+TGG 0.409905 4.4:-32580618 None:intergenic
CGGTGAGAATGATGATGAAT+TGG 0.410275 4.4:+32580473 MS.gene006572:CDS
CATGAAGCCCGGACTATTCA+TGG 0.411920 4.4:-32579887 None:intergenic
GATGAACTACTGCTATCTAA+TGG 0.419987 4.4:-32580177 None:intergenic
ATTTGTTAAAGAATATCAAT+AGG 0.421836 4.4:+32579602 MS.gene006572:CDS
TGAGCGAATTCCCAGCGGTC+GGG 0.422148 4.4:-32579553 None:intergenic
ATCGTTGATTCCTGGAAGCT+TGG 0.423687 4.4:-32580571 None:intergenic
GTGTGGAAGTGGGTAAGTTT+GGG 0.436120 4.4:+32579695 MS.gene006572:CDS
AACCGAATCGATCGTTTCAT+GGG 0.436953 4.4:+32578696 MS.gene006572:CDS
CAAGATGTGTTTGGAGAAAT+CGG 0.437903 4.4:-32578755 None:intergenic
GTTGCCCAACATTTACCAAT+TGG 0.441846 4.4:-32579810 None:intergenic
AATGAAAATTCCAAGCTTCC+AGG 0.444171 4.4:+32580561 MS.gene006572:CDS
GAATGCATCAAGGTTCTTGA+TGG 0.448987 4.4:-32580127 None:intergenic
GATAACCAATTGGTAAATGT+TGG 0.452224 4.4:+32579805 MS.gene006572:CDS
CGGAAATCTACTCATGCAAA+TGG 0.457161 4.4:+32579625 MS.gene006572:CDS
AGCAAAGGCCATGAATAGTC+CGG 0.460979 4.4:+32579879 MS.gene006572:CDS
GGATCGCGAATTATGACAGC+TGG 0.461443 4.4:+32578610 None:intergenic
GAAGATTCAACAAGAAAAGA+TGG 0.461849 4.4:+32580707 MS.gene006572:CDS
AAAGAGGCGGCTCCAGGGGC+AGG 0.463211 4.4:+32579963 MS.gene006572:CDS
ATAACCAATTGGTAAATGTT+GGG 0.467159 4.4:+32579806 MS.gene006572:CDS
AAAGTGTTACACCAGAAACT+TGG 0.470921 4.4:-32580203 None:intergenic
AACTATCTTCATCCTGCCCC+TGG 0.473972 4.4:-32579975 None:intergenic
AGGAATCAACGATGAATTCT+GGG 0.476706 4.4:+32580581 MS.gene006572:CDS
TGTTGGGTCATGTGTGGAAG+TGG 0.480175 4.4:+32579684 MS.gene006572:CDS
AGCCGCAGAACCCACCTGTT+GGG 0.484089 4.4:+32579668 MS.gene006572:CDS
GAAAGGTTGACCCCGACCGC+TGG 0.485865 4.4:+32579542 MS.gene006572:CDS
AAGAGGTTGAAAGACTGAAA+AGG 0.488568 4.4:+32579725 MS.gene006572:CDS
TATTTCTGGGCAGTCATGTA+TGG 0.490052 4.4:+32580239 MS.gene006572:CDS
GTTCAATTGTCTGACGAAGC+TGG 0.491130 4.4:-32578806 None:intergenic
AGAGGTTGAAAGACTGAAAA+GGG 0.491243 4.4:+32579726 MS.gene006572:CDS
GACTATTCATGGCCTTTGCT+AGG 0.492617 4.4:-32579876 None:intergenic
GAGCGAATTCCCAGCGGTCG+GGG 0.492924 4.4:-32579552 None:intergenic
ACAATCGACTGATAACCAAT+TGG 0.498524 4.4:+32579795 MS.gene006572:CDS
ACCCCATGAAACGATCGATT+CGG 0.504242 4.4:-32578698 None:intergenic
ACTTCCACACATGACCCAAC+AGG 0.505692 4.4:-32579682 None:intergenic
TCGTCTGTGTCTCCAGTAAG+TGG 0.508093 4.4:-32580624 None:intergenic
GAAGTTAAAGGTAGTTCTCT+AGG 0.508241 4.4:+32580645 MS.gene006572:CDS
TTCTGGTGTAACACTTTCTG+AGG 0.510063 4.4:+32580209 MS.gene006572:CDS
CAAAATCCAAAATATTGTCT+TGG 0.518046 4.4:-32580383 None:intergenic
GTACCAGCCTTCTATTAATG+AGG 0.518867 4.4:+32580044 MS.gene006572:CDS
TTGTACCTCAGACATGCTAT+TGG 0.521476 4.4:-32580295 None:intergenic
GTTCCATCTGTTGAGTAAGA+TGG 0.529932 4.4:-32580749 None:intergenic
CGCTGGGAATTCGCTCATGA+AGG 0.530003 4.4:+32579559 MS.gene006572:CDS
CAGCCTCATTAATAGAAGGC+TGG 0.531526 4.4:-32580047 None:intergenic
CGTCCATCAAGAGGATTATG+TGG 0.541079 4.4:-32580012 None:intergenic
CATCAATAAGGAATGCATCA+AGG 0.541216 4.4:-32580137 None:intergenic
AAAGGTTGACCCCGACCGCT+GGG 0.545860 4.4:+32579543 MS.gene006572:CDS
GAAAATTGAGCCATGAAGCC+CGG 0.546699 4.4:-32579898 None:intergenic
ACAGATGGAACTTCTTGCAT+CGG 0.548170 4.4:+32580761 MS.gene006572:CDS
AAGCCGCAGAACCCACCTGT+TGG 0.557194 4.4:+32579667 MS.gene006572:CDS
TAAGTTTGGGCTTGATGAAG+AGG 0.559114 4.4:+32579708 MS.gene006572:CDS
GCAAAGGCCATGAATAGTCC+GGG 0.565582 4.4:+32579880 MS.gene006572:CDS
AATGAACAGTTCTGCAAGGG+TGG 0.569475 4.4:+32580098 MS.gene006572:CDS
ATGAGCGAATTCCCAGCGGT+CGG 0.572798 4.4:-32579554 None:intergenic
GATGATGTCGTTCCTAGCAA+AGG 0.573149 4.4:+32579864 MS.gene006572:CDS
ATATGGTTTGACTAAAGACC+AGG 0.574236 4.4:+32580668 MS.gene006572:CDS
GGACCATCTTACTCAACAGA+TGG 0.577724 4.4:+32580746 MS.gene006572:CDS
AAGTGTTACACCAGAAACTT+GGG 0.578836 4.4:-32580202 None:intergenic
CTATCTAATGGAATGGCTGA+AGG 0.583304 4.4:-32580165 None:intergenic
TCCACACATGACCCAACAGG+TGG 0.591825 4.4:-32579679 None:intergenic
AGGATTATGTGGATTCTTGG+TGG 0.592009 4.4:-32580001 None:intergenic
GACCCAACAGGTGGGTTCTG+CGG 0.593008 4.4:-32579670 None:intergenic
AGTAATAGACATGTTACCGC+TGG 0.593286 4.4:+32579937 MS.gene006572:CDS
CGTCTGTGTCTCCAGTAAGT+GGG 0.594211 4.4:-32580623 None:intergenic
ACACAATCAGTCAACACAGC+AGG 0.595480 4.4:-32580324 None:intergenic
TGTAAATTCTGGAAATTCAG+CGG 0.596634 4.4:-32580352 None:intergenic
TGCATCAAGGTTCTTGATGG+AGG 0.607948 4.4:-32580124 None:intergenic
AGAGTCATCAACCATATCGT+AGG 0.608336 4.4:-32578671 None:intergenic
TGGGCAACGTGTGCAAGTGA+TGG 0.618938 4.4:+32579825 MS.gene006572:CDS
TTAACAACACGTCCATCAAG+AGG 0.623081 4.4:-32580021 None:intergenic
ACTACTGCTATCTAATGGAA+TGG 0.639316 4.4:-32580172 None:intergenic
CCCACCTGTTGGGTCATGTG+TGG 0.640259 4.4:+32579678 MS.gene006572:CDS
GATCGCGAATTATGACAGCT+GGG 0.642062 4.4:+32578611 None:intergenic
TGTTAAAGAATATCAATAGG+CGG 0.647648 4.4:+32579605 MS.gene006572:CDS
TATGACCAATAGCATGTCTG+AGG 0.654015 4.4:+32580290 MS.gene006572:CDS
TGCAAATGAACAGTTCTGCA+AGG 0.655638 4.4:+32580094 MS.gene006572:CDS
CATGACTGCCCAGAAATAGG+TGG 0.662832 4.4:-32580234 None:intergenic
GTTGGGTCATGTGTGGAAGT+GGG 0.670827 4.4:+32579685 MS.gene006572:CDS
ACCGAATCGATCGTTTCATG+GGG 0.677166 4.4:+32578697 MS.gene006572:CDS
GCAAATGAACAGTTCTGCAA+GGG 0.681864 4.4:+32580095 MS.gene006572:CDS
GATGGATAAGATACAGCACA+TGG 0.683473 4.4:+32580725 MS.gene006572:CDS
CCACACATGACCCAACAGGT+GGG 0.694523 4.4:-32579678 None:intergenic
TGTTACACCAGAAACTTGGG+TGG 0.706092 4.4:-32580199 None:intergenic
GGTGGAATTGAAAACGACGA+AGG 0.715764 4.4:-32578734 None:intergenic
GATGTGTTTGGAGAAATCGG+TGG 0.725221 4.4:-32578752 None:intergenic
CTTCATGAGCGAATTCCCAG+CGG 0.750748 4.4:-32579558 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCTTAGAAAATATTCAT+CGG - chr4.4:32579156-32579175 None:intergenic 15.0%
!!! ATTTGTTAAAGAATATCAAT+AGG + chr4.4:32579602-32579621 MS.gene006572:CDS 15.0%
!!! TTTTCAGATTTTCTTAATTA+GGG + chr4.4:32578853-32578872 MS.gene006572:intron 15.0%
!! ATGTTATTAAAAAAGCAAGA+AGG - chr4.4:32579419-32579438 None:intergenic 20.0%
!! CTAATTAAGAAAATCTGAAA+AGG - chr4.4:32578854-32578873 None:intergenic 20.0%
!! TAACATACATTTAATAATGC+TGG + chr4.4:32579433-32579452 MS.gene006572:intron 20.0%
!!! CTTTTCAGATTTTCTTAATT+AGG + chr4.4:32578852-32578871 MS.gene006572:intron 20.0%
!!! GATGAATATTTTCTAAGAAT+TGG + chr4.4:32579155-32579174 MS.gene006572:intron 20.0%
!!! TTAAGTAGTAATAGGATTTT+AGG + chr4.4:32579292-32579311 MS.gene006572:intron 20.0%
!!! TTGTTGTGTTTGAAATATTT+AGG - chr4.4:32578781-32578800 None:intergenic 20.0%
! ATAACCAATTGGTAAATGTT+GGG + chr4.4:32579806-32579825 MS.gene006572:CDS 25.0%
! CAAAATCCAAAATATTGTCT+TGG - chr4.4:32580386-32580405 None:intergenic 25.0%
! GTAATTAGAGAAGTTACATT+CGG + chr4.4:32579040-32579059 MS.gene006572:intron 25.0%
! GTTCAGATCATATTAACTTA+TGG + chr4.4:32578896-32578915 MS.gene006572:intron 25.0%
! TAATTAGAGAAGTTACATTC+GGG + chr4.4:32579041-32579060 MS.gene006572:intron 25.0%
! TGTTAAAGAATATCAATAGG+CGG + chr4.4:32579605-32579624 MS.gene006572:CDS 25.0%
!! AATCCTGATCAAAATTTTGT+GGG + chr4.4:32580444-32580463 MS.gene006572:CDS 25.0%
!! CTTAATTGTAATTTGGTGAT+TGG + chr4.4:32579001-32579020 MS.gene006572:intron 25.0%
!! TTATGGACGAAGATTTTATT+AGG + chr4.4:32578913-32578932 MS.gene006572:intron 25.0%
!! TTGTATACTTTTAATCATGC+TGG + chr4.4:32579468-32579487 MS.gene006572:intron 25.0%
!!! AATTCTGGGAACAATTTTTT+AGG + chr4.4:32580595-32580614 MS.gene006572:CDS 25.0%
!!! AGTAATAGGATTTTAGGTTA+TGG + chr4.4:32579298-32579317 MS.gene006572:intron 25.0%
!!! ATCTTTTTTATTCACAAGCA+GGG + chr4.4:32579514-32579533 MS.gene006572:intron 25.0%
!!! ATTTTCTAAGAATTGGATGT+AGG + chr4.4:32579162-32579181 MS.gene006572:intron 25.0%
!!! CAAGACAATATTTTGGATTT+TGG + chr4.4:32580384-32580403 MS.gene006572:CDS 25.0%
!!! TATCTTTTTTATTCACAAGC+AGG + chr4.4:32579513-32579532 MS.gene006572:intron 25.0%
!!! TTTTAGGTTATGGTTAAGAT+AGG + chr4.4:32579308-32579327 MS.gene006572:intron 25.0%
ATATTTAGGCAAGATGTGTT+TGG - chr4.4:32578767-32578786 None:intergenic 30.0%
ATTAAAAAAGCAAGAAGGAG+AGG - chr4.4:32579414-32579433 None:intergenic 30.0%
GAAGATTCAACAAGAAAAGA+TGG + chr4.4:32580707-32580726 MS.gene006572:CDS 30.0%
GACTGCAATTAAGTAGTAAT+AGG + chr4.4:32579284-32579303 MS.gene006572:intron 30.0%
GATAACCAATTGGTAAATGT+TGG + chr4.4:32579805-32579824 MS.gene006572:CDS 30.0%
TAACTAATGAATCCCCAATA+GGG - chr4.4:32579336-32579355 None:intergenic 30.0%
TGTTAGAACTGAATTCTACA+CGG + chr4.4:32579248-32579267 MS.gene006572:intron 30.0%
! ATCCTGATCAAAATTTTGTG+GGG + chr4.4:32580445-32580464 MS.gene006572:CDS 30.0%
! GAATCCTGATCAAAATTTTG+TGG + chr4.4:32580443-32580462 MS.gene006572:CDS 30.0%
! TGTAAATTCTGGAAATTCAG+CGG - chr4.4:32580355-32580374 None:intergenic 30.0%
AACTGTTCATTTGCAACATC+TGG - chr4.4:32580089-32580108 None:intergenic 35.0%
AAGAGGATTATGTGGATTCT+TGG - chr4.4:32580007-32580026 None:intergenic 35.0%
AAGAGGTTGAAAGACTGAAA+AGG + chr4.4:32579725-32579744 MS.gene006572:CDS 35.0%
AAGTGTTACACCAGAAACTT+GGG - chr4.4:32580205-32580224 None:intergenic 35.0%
AATCCACATAATCCTCTTGA+TGG + chr4.4:32580009-32580028 MS.gene006572:CDS 35.0%
AATGAAAATTCCAAGCTTCC+AGG + chr4.4:32580561-32580580 MS.gene006572:CDS 35.0%
ACAATCGACTGATAACCAAT+TGG + chr4.4:32579795-32579814 MS.gene006572:CDS 35.0%
ACTACTGCTATCTAATGGAA+TGG - chr4.4:32580175-32580194 None:intergenic 35.0%
AGAGGTTGAAAGACTGAAAA+GGG + chr4.4:32579726-32579745 MS.gene006572:CDS 35.0%
AGGAATCAACGATGAATTCT+GGG + chr4.4:32580581-32580600 MS.gene006572:CDS 35.0%
ATATGGTTTGACTAAAGACC+AGG + chr4.4:32580668-32580687 MS.gene006572:CDS 35.0%
CAAGATGTGTTTGGAGAAAT+CGG - chr4.4:32578758-32578777 None:intergenic 35.0%
CATCAATAAGGAATGCATCA+AGG - chr4.4:32580140-32580159 None:intergenic 35.0%
CATTTGCAAACTGCAAAAAG+TGG - chr4.4:32579230-32579249 None:intergenic 35.0%
CTAACTAATGAATCCCCAAT+AGG - chr4.4:32579337-32579356 None:intergenic 35.0%
CTATTGGTCATACAACTTAC+AGG - chr4.4:32580282-32580301 None:intergenic 35.0%
CTGAAGGAACATCATCAATA+AGG - chr4.4:32580152-32580171 None:intergenic 35.0%
GAACGCGCTTAATTGTAATT+TGG + chr4.4:32578994-32579013 MS.gene006572:intron 35.0%
GAAGTTAAAGGTAGTTCTCT+AGG + chr4.4:32580645-32580664 MS.gene006572:CDS 35.0%
GATGAACTACTGCTATCTAA+TGG - chr4.4:32580180-32580199 None:intergenic 35.0%
GGTAAAGTAAGAACATACGT+AGG - chr4.4:32578833-32578852 None:intergenic 35.0%
GTTAAGATAGGTACCCTATT+GGG + chr4.4:32579320-32579339 MS.gene006572:intron 35.0%
TAACATTTCCAAGAGTTTCG+TGG + chr4.4:32579185-32579204 MS.gene006572:intron 35.0%
TCTTTGCTAATGACAATTCC+TGG - chr4.4:32580689-32580708 None:intergenic 35.0%
TTAAGATAGGTACCCTATTG+GGG + chr4.4:32579321-32579340 MS.gene006572:intron 35.0%
TTTGCAGCCTCATTAATAGA+AGG - chr4.4:32580054-32580073 None:intergenic 35.0%
! AAAGTGTTACACCAGAAACT+TGG - chr4.4:32580206-32580225 None:intergenic 35.0%
! AGCACACCAAGACAATATTT+TGG + chr4.4:32580377-32580396 MS.gene006572:CDS 35.0%
! CGCTCATGAAGGATTTTTAA+GGG + chr4.4:32579570-32579589 MS.gene006572:CDS 35.0%
! TCGCTCATGAAGGATTTTTA+AGG + chr4.4:32579569-32579588 MS.gene006572:CDS 35.0%
! TGTAATTTGGTGATTGGAGA+GGG + chr4.4:32579007-32579026 MS.gene006572:intron 35.0%
! TTGTAATTTGGTGATTGGAG+AGG + chr4.4:32579006-32579025 MS.gene006572:intron 35.0%
!! AAAGGTAGTTCTCTAGGATA+TGG + chr4.4:32580651-32580670 MS.gene006572:CDS 35.0%
!! GCTCATGAAGGATTTTTAAG+GGG + chr4.4:32579571-32579590 MS.gene006572:CDS 35.0%
!! TGATATCAGCTGTTTTGGAT+GGG + chr4.4:32580499-32580518 MS.gene006572:CDS 35.0%
!! TTGGATTTTGGTGAAGTTCA+AGG + chr4.4:32580396-32580415 MS.gene006572:CDS 35.0%
AACCGAATCGATCGTTTCAT+GGG + chr4.4:32578696-32578715 MS.gene006572:CDS 40.0%
ACAGATGGAACTTCTTGCAT+CGG + chr4.4:32580761-32580780 MS.gene006572:CDS 40.0%
ACAGTAACAGTAACACTAGC+AGG - chr4.4:32579106-32579125 None:intergenic 40.0%
AGAGTCATCAACCATATCGT+AGG - chr4.4:32578674-32578693 None:intergenic 40.0%
AGGATTATGTGGATTCTTGG+TGG - chr4.4:32580004-32580023 None:intergenic 40.0%
AGTAATAGACATGTTACCGC+TGG + chr4.4:32579937-32579956 MS.gene006572:CDS 40.0%
ATACATGACTGCCCAGAAAT+AGG - chr4.4:32580240-32580259 None:intergenic 40.0%
ATGTTACCGCTGGTAAAAAG+AGG + chr4.4:32579947-32579966 MS.gene006572:CDS 40.0%
ATTTCCAAGAGTTTCGTGGA+AGG + chr4.4:32579189-32579208 MS.gene006572:intron 40.0%
CAAAGTCAAGATACTTGTCC+TGG + chr4.4:32579369-32579388 MS.gene006572:intron 40.0%
CAGGAATCAACGATGAATTC+TGG + chr4.4:32580580-32580599 MS.gene006572:CDS 40.0%
CCTAAGCAAAACCTACGATA+TGG + chr4.4:32578660-32578679 MS.gene006572:CDS 40.0%
CGGAAATCTACTCATGCAAA+TGG + chr4.4:32579625-32579644 MS.gene006572:CDS 40.0%
CTATCTAATGGAATGGCTGA+AGG - chr4.4:32580168-32580187 None:intergenic 40.0%
GAATGCATCAAGGTTCTTGA+TGG - chr4.4:32580130-32580149 None:intergenic 40.0%
GATGGATAAGATACAGCACA+TGG + chr4.4:32580725-32580744 MS.gene006572:CDS 40.0%
GCAAATGAACAGTTCTGCAA+GGG + chr4.4:32580095-32580114 MS.gene006572:CDS 40.0%
GGTTAAGATAGGTACCCTAT+TGG + chr4.4:32579319-32579338 MS.gene006572:intron 40.0%
GTACCAGCCTTCTATTAATG+AGG + chr4.4:32580044-32580063 MS.gene006572:CDS 40.0%
GTTACATTCGGGATGCATTT+CGG + chr4.4:32579052-32579071 MS.gene006572:intron 40.0%
GTTCCATCTGTTGAGTAAGA+TGG - chr4.4:32580752-32580771 None:intergenic 40.0%
GTTGCCCAACATTTACCAAT+TGG - chr4.4:32579813-32579832 None:intergenic 40.0%
TAAGTTTGGGCTTGATGAAG+AGG + chr4.4:32579708-32579727 MS.gene006572:CDS 40.0%
TATGACCAATAGCATGTCTG+AGG + chr4.4:32580290-32580309 MS.gene006572:CDS 40.0%
TGCAAATGAACAGTTCTGCA+AGG + chr4.4:32580094-32580113 MS.gene006572:CDS 40.0%
TTAACAACACGTCCATCAAG+AGG - chr4.4:32580024-32580043 None:intergenic 40.0%
TTCTGGTGTAACACTTTCTG+AGG + chr4.4:32580209-32580228 MS.gene006572:CDS 40.0%
TTGTACCTCAGACATGCTAT+TGG - chr4.4:32580298-32580317 None:intergenic 40.0%
! CAAAATTTTGTGGGGTCTGT+CGG + chr4.4:32580453-32580472 MS.gene006572:CDS 40.0%
! CCATATCGTAGGTTTTGCTT+AGG - chr4.4:32578663-32578682 None:intergenic 40.0%
! CGGTGAGAATGATGATGAAT+TGG + chr4.4:32580473-32580492 MS.gene006572:CDS 40.0%
! GGATGTGATATCAGCTGTTT+TGG + chr4.4:32580494-32580513 MS.gene006572:CDS 40.0%
! TCGTAGGTTTTGCTTAGGAA+CGG - chr4.4:32578658-32578677 None:intergenic 40.0%
! TGCTAGTGTTACTGTTACTG+TGG + chr4.4:32579105-32579124 MS.gene006572:intron 40.0%
! TGGTGTGCTGTTGTAAATTC+TGG - chr4.4:32580366-32580385 None:intergenic 40.0%
! TTTTGGTGAAGTTCAAGGAC+TGG + chr4.4:32580401-32580420 MS.gene006572:CDS 40.0%
!! CAGAATTCATCGTTGATTCC+TGG - chr4.4:32580582-32580601 None:intergenic 40.0%
!! GACCCCACAAAATTTTGATC+AGG - chr4.4:32580450-32580469 None:intergenic 40.0%
!! GTGATATCAGCTGTTTTGGA+TGG + chr4.4:32580498-32580517 MS.gene006572:CDS 40.0%
!! TATTTCTGGGCAGTCATGTA+TGG + chr4.4:32580239-32580258 MS.gene006572:CDS 40.0%
!!! TCACAAGCAGGGTTTTAGAA+AGG + chr4.4:32579525-32579544 MS.gene006572:intron 40.0%
AATGAACAGTTCTGCAAGGG+TGG + chr4.4:32580098-32580117 MS.gene006572:CDS 45.0%
ACACAATCAGTCAACACAGC+AGG - chr4.4:32580327-32580346 None:intergenic 45.0%
ACCCCATGAAACGATCGATT+CGG - chr4.4:32578701-32578720 None:intergenic 45.0%
ACCGAATCGATCGTTTCATG+GGG + chr4.4:32578697-32578716 MS.gene006572:CDS 45.0%
AGCAAAGGCCATGAATAGTC+CGG + chr4.4:32579879-32579898 MS.gene006572:CDS 45.0%
AGTTCATCCACCCAAGTTTC+TGG + chr4.4:32580192-32580211 MS.gene006572:CDS 45.0%
ATATTCATCGGTACACCGAG+CGG - chr4.4:32579144-32579163 None:intergenic 45.0%
CAACCGAATCGATCGTTTCA+TGG + chr4.4:32578695-32578714 MS.gene006572:CDS 45.0%
CAGCCTCATTAATAGAAGGC+TGG - chr4.4:32580050-32580069 None:intergenic 45.0%
CATTCCTTCCACGAAACTCT+TGG - chr4.4:32579196-32579215 None:intergenic 45.0%
CGTCCATCAAGAGGATTATG+TGG - chr4.4:32580015-32580034 None:intergenic 45.0%
GAAAATTGAGCCATGAAGCC+CGG - chr4.4:32579901-32579920 None:intergenic 45.0%
GACTATTCATGGCCTTTGCT+AGG - chr4.4:32579879-32579898 None:intergenic 45.0%
GATGATGTCGTTCCTAGCAA+AGG + chr4.4:32579864-32579883 MS.gene006572:CDS 45.0%
GATGTGTTTGGAGAAATCGG+TGG - chr4.4:32578755-32578774 None:intergenic 45.0%
GGACCATCTTACTCAACAGA+TGG + chr4.4:32580746-32580765 MS.gene006572:CDS 45.0%
GGAGACACAGACGAAGTTAA+AGG + chr4.4:32580633-32580652 MS.gene006572:CDS 45.0%
GGTGGAATTGAAAACGACGA+AGG - chr4.4:32578737-32578756 None:intergenic 45.0%
GTGTGGAAGTGGGTAAGTTT+GGG + chr4.4:32579695-32579714 MS.gene006572:CDS 45.0%
GTTCAATTGTCTGACGAAGC+TGG - chr4.4:32578809-32578828 None:intergenic 45.0%
TGCAAACTGCAAAAAGTGGC+TGG - chr4.4:32579226-32579245 None:intergenic 45.0%
TGCATCAAGGTTCTTGATGG+AGG - chr4.4:32580127-32580146 None:intergenic 45.0%
TGTGTGGAAGTGGGTAAGTT+TGG + chr4.4:32579694-32579713 MS.gene006572:CDS 45.0%
TGTTACACCAGAAACTTGGG+TGG - chr4.4:32580202-32580221 None:intergenic 45.0%
TTACCGCTGGTAAAAAGAGG+CGG + chr4.4:32579950-32579969 MS.gene006572:CDS 45.0%
! CTGAGGTTCCACCTATTTCT+GGG + chr4.4:32580226-32580245 MS.gene006572:CDS 45.0%
! TAGGTTTTGCTTAGGAACGG+TGG - chr4.4:32578655-32578674 None:intergenic 45.0%
! TCTGAGGTTCCACCTATTTC+TGG + chr4.4:32580225-32580244 MS.gene006572:CDS 45.0%
!! ATCGTTGATTCCTGGAAGCT+TGG - chr4.4:32580574-32580593 None:intergenic 45.0%
AACTATCTTCATCCTGCCCC+TGG - chr4.4:32579978-32579997 None:intergenic 50.0%
ACTTCCACACATGACCCAAC+AGG - chr4.4:32579685-32579704 None:intergenic 50.0%
AGATACTTGTCCTGGTGCTC+TGG + chr4.4:32579377-32579396 MS.gene006572:intron 50.0%
CATGAAGCCCGGACTATTCA+TGG - chr4.4:32579890-32579909 None:intergenic 50.0%
CATGAATAGTCCGGGCTTCA+TGG + chr4.4:32579888-32579907 MS.gene006572:CDS 50.0%
CATGACTGCCCAGAAATAGG+TGG - chr4.4:32580237-32580256 None:intergenic 50.0%
CGTCTGTGTCTCCAGTAAGT+GGG - chr4.4:32580626-32580645 None:intergenic 50.0%
CTTCATGAGCGAATTCCCAG+CGG - chr4.4:32579561-32579580 None:intergenic 50.0%
GCAAAGGCCATGAATAGTCC+GGG + chr4.4:32579880-32579899 MS.gene006572:CDS 50.0%
GTTGGGTCATGTGTGGAAGT+GGG + chr4.4:32579685-32579704 MS.gene006572:CDS 50.0%
TCGTCTGTGTCTCCAGTAAG+TGG - chr4.4:32580627-32580646 None:intergenic 50.0%
TGTTGGGTCATGTGTGGAAG+TGG + chr4.4:32579684-32579703 MS.gene006572:CDS 50.0%
TTTAGGCCAAGCCCACTTAC+TGG + chr4.4:32580612-32580631 MS.gene006572:CDS 50.0%
TTTGGATGGGACACGGTCTT+TGG + chr4.4:32580512-32580531 MS.gene006572:CDS 50.0%
! AAATTCAGCACCAGAGCACC+AGG - chr4.4:32579390-32579409 None:intergenic 50.0%
!! CAGCTGTTTTGGATGGGACA+CGG + chr4.4:32580505-32580524 MS.gene006572:CDS 50.0%
ATGAGCGAATTCCCAGCGGT+CGG - chr4.4:32579557-32579576 None:intergenic 55.0%
CCACACATGACCCAACAGGT+GGG - chr4.4:32579681-32579700 None:intergenic 55.0%
CGCTGGGAATTCGCTCATGA+AGG + chr4.4:32579559-32579578 MS.gene006572:CDS 55.0%
GGTAAAAAGAGGCGGCTCCA+GGG + chr4.4:32579958-32579977 MS.gene006572:CDS 55.0%
GTAAAAAGAGGCGGCTCCAG+GGG + chr4.4:32579959-32579978 MS.gene006572:CDS 55.0%
TCCACACATGACCCAACAGG+TGG - chr4.4:32579682-32579701 None:intergenic 55.0%
TGGGCAACGTGTGCAAGTGA+TGG + chr4.4:32579825-32579844 MS.gene006572:CDS 55.0%
TGGTAAAAAGAGGCGGCTCC+AGG + chr4.4:32579957-32579976 MS.gene006572:CDS 55.0%
! GAGCCGCCTCTTTTTACCAG+CGG - chr4.4:32579956-32579975 None:intergenic 55.0%
! GTGTCTCCAGTAAGTGGGCT+TGG - chr4.4:32580621-32580640 None:intergenic 55.0%
AAAGGTTGACCCCGACCGCT+GGG + chr4.4:32579543-32579562 MS.gene006572:CDS 60.0%
AAGCCGCAGAACCCACCTGT+TGG + chr4.4:32579667-32579686 MS.gene006572:CDS 60.0%
AGCCGCAGAACCCACCTGTT+GGG + chr4.4:32579668-32579687 MS.gene006572:CDS 60.0%
CCCACCTGTTGGGTCATGTG+TGG + chr4.4:32579678-32579697 MS.gene006572:CDS 60.0%
GGTGATTGGAGAGGGAGCTG+TGG + chr4.4:32579015-32579034 MS.gene006572:intron 60.0%
TGAGCGAATTCCCAGCGGTC+GGG - chr4.4:32579556-32579575 None:intergenic 60.0%
! GACCCAACAGGTGGGTTCTG+CGG - chr4.4:32579673-32579692 None:intergenic 60.0%
CGGTACACCGAGCGGAAGCA+TGG - chr4.4:32579136-32579155 None:intergenic 65.0%
GAAAGGTTGACCCCGACCGC+TGG + chr4.4:32579542-32579561 MS.gene006572:CDS 65.0%
GAGCGAATTCCCAGCGGTCG+GGG - chr4.4:32579555-32579574 None:intergenic 65.0%
! GGTGCTGCCATGCTTCCGCT+CGG + chr4.4:32579126-32579145 MS.gene006572:intron 65.0%
AAAGAGGCGGCTCCAGGGGC+AGG + chr4.4:32579963-32579982 MS.gene006572:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 32578622 32580803 32578622 ID=MS.gene006572
chr4.4 mRNA 32578622 32580803 32578622 ID=MS.gene006572.t1;Parent=MS.gene006572
chr4.4 exon 32578622 32578834 32578622 ID=MS.gene006572.t1.exon1;Parent=MS.gene006572.t1
chr4.4 CDS 32578622 32578834 32578622 ID=cds.MS.gene006572.t1;Parent=MS.gene006572.t1
chr4.4 exon 32579535 32580803 32579535 ID=MS.gene006572.t1.exon2;Parent=MS.gene006572.t1
chr4.4 CDS 32579535 32580803 32579535 ID=cds.MS.gene006572.t1;Parent=MS.gene006572.t1
Gene Sequence

>MS.gene006572

ATGACAGCTGGGATCGCGAATTATGTATTACCACCGTTCCTAAGCAAAACCTACGATATGGTTGATGACTCTTCAACCGAATCGATCGTTTCATGGGGTAAAAACGACAACACCTTCGTCGTTTTCAATTCCACCGATTTCTCCAAACACATCTTGCCTAAATATTTCAAACACAACAACTTCTCCAGCTTCGTCAGACAATTGAACACCTACGTATGTTCTTACTTTACCTTTTCAGATTTTCTTAATTAGGGTTTTACAGTAATTCATTTTTGTTCAGATCATATTAACTTATGGACGAAGATTTTATTAGGTTTTGTAATTGATAGAATTTATCTATTTATTCATAAATTGCTCAGTGATTATAATTGTGAACGCGCTTAATTGTAATTTGGTGATTGGAGAGGGAGCTGTGGTTGTAATTAGAGAAGTTACATTCGGGATGCATTTCGGATTGTAAATGCAAAGATACAGTTAGCTACCTGCTAGTGTTACTGTTACTGTGGTGCTGCCATGCTTCCGCTCGGTGTACCGATGAATATTTTCTAAGAATTGGATGTAGGTAACATTTCCAAGAGTTTCGTGGAAGGAATGTTTCAAACCAGCCACTTTTTGCAGTTTGCAAATGTTAGAACTGAATTCTACACGGTAAGTAGAATAGTGACTGCAATTAAGTAGTAATAGGATTTTAGGTTATGGTTAAGATAGGTACCCTATTGGGGATTCATTAGTTAGTTAGTTAGTTATCAAAGTCAAGATACTTGTCCTGGTGCTCTGGTGCTGAATTTACCTCTCCTTCTTGCTTTTTTAATAACATACATTTAATAATGCTGGACTATTTGTATATTGTATACTTTTAATCATGCTGGAGTATTTACTCATCATATCAACTATCTTTTTTATTCACAAGCAGGGTTTTAGAAAGGTTGACCCCGACCGCTGGGAATTCGCTCATGAAGGATTTTTAAGGGGTCAAAAACATTTGTTAAAGAATATCAATAGGCGGAAATCTACTCATGCAAATGGTAATAATCAGCAGCTATCTAAGCCGCAGAACCCACCTGTTGGGTCATGTGTGGAAGTGGGTAAGTTTGGGCTTGATGAAGAGGTTGAAAGACTGAAAAGGGACAAGAATGTTCTTATGCAAGAACTTGTTAAGTTAAGACAGCAACAACAATCGACTGATAACCAATTGGTAAATGTTGGGCAACGTGTGCAAGTGATGGAGCAGCGTCAGCAACAGATGATGTCGTTCCTAGCAAAGGCCATGAATAGTCCGGGCTTCATGGCTCAATTTTCACAGCAGCAGAATGAAAGTAATAGACATGTTACCGCTGGTAAAAAGAGGCGGCTCCAGGGGCAGGATGAAGATAGTTTAGCCACCAAGAATCCACATAATCCTCTTGATGGACGTGTTGTTAAGTACCAGCCTTCTATTAATGAGGCTGCAAAAACATTATTTAACCAGATGTTGCAAATGAACAGTTCTGCAAGGGTGGATTCCTCCATCAAGAACCTTGATGCATTCCTTATTGATGATGTTCCTTCAGCCATTCCATTAGATAGCAGTAGTTCATCCACCCAAGTTTCTGGTGTAACACTTTCTGAGGTTCCACCTATTTCTGGGCAGTCATGTATGGCAGTAGAATCTCAATTTCCTGTAAGTTGTATGACCAATAGCATGTCTGAGGTACAATCTTCACCTGCTGTGTTGACTGATTGTGTCAAAACCGCTGAATTTCCAGAATTTACAACAGCACACCAAGACAATATTTTGGATTTTGGTGAAGTTCAAGGACTGGCTACAGAAAGCAGCTTCATGAATCCTGATCAAAATTTTGTGGGGTCTGTCGGTGAGAATGATGATGAATTGGATGTGATATCAGCTGTTTTGGATGGGACACGGTCTTTGGAAGCTGATGCTTTTTCTTCTGATGCGAATGAAAATTCCAAGCTTCCAGGAATCAACGATGAATTCTGGGAACAATTTTTTAGGCCAAGCCCACTTACTGGAGACACAGACGAAGTTAAAGGTAGTTCTCTAGGATATGGTTTGACTAAAGACCAGGAATTGTCATTAGCAAAGAAGATTCAACAAGAAAAGATGGATAAGATACAGCACATGGACCATCTTACTCAACAGATGGAACTTCTTGCATCGGATTCAACATTGTGCATATAA
Protein sequence

>MS.gene006572.t1

MTAGIANYVLPPFLSKTYDMVDDSSTESIVSWGKNDNTFVVFNSTDFSKHILPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFAHEGFLRGQKHLLKNINRRKSTHANGNNQQLSKPQNPPVGSCVEVGKFGLDEEVERLKRDKNVLMQELVKLRQQQQSTDNQLVNVGQRVQVMEQRQQQMMSFLAKAMNSPGFMAQFSQQQNESNRHVTAGKKRRLQGQDEDSLATKNPHNPLDGRVVKYQPSINEAAKTLFNQMLQMNSSARVDSSIKNLDAFLIDDVPSAIPLDSSSSSTQVSGVTLSEVPPISGQSCMAVESQFPVSCMTNSMSEVQSSPAVLTDCVKTAEFPEFTTAHQDNILDFGEVQGLATESSFMNPDQNFVGSVGENDDELDVISAVLDGTRSLEADAFSSDANENSKLPGINDEFWEQFFRPSPLTGDTDEVKGSSLGYGLTKDQELSLAKKIQQEKMDKIQHMDHLTQQMELLASDSTLCI