AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006573

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006573.t1 MTR_4g077970 98.174 493 9 0 1 493 1 493 0.0 1006
MS.gene006573.t1 MTR_4g077970 97.977 346 7 0 148 493 1 346 0.0 694
MS.gene006573.t1 MTR_5g029680 46.800 500 214 17 11 489 31 499 8.41e-128 381
MS.gene006573.t1 MTR_3g104550 54.260 223 90 3 11 233 45 255 3.83e-74 239
MS.gene006573.t1 MTR_7g095680 56.522 207 87 1 11 217 48 251 4.68e-74 238
MS.gene006573.t1 MTR_1g102860 55.714 210 82 4 11 217 39 240 1.54e-69 225
MS.gene006573.t1 MTR_1g061170 41.254 303 151 6 10 309 47 325 2.54e-69 229
MS.gene006573.t1 MTR_1g071360 51.643 213 83 3 12 221 32 227 2.96e-63 209
MS.gene006573.t1 MTR_1g071360 51.174 213 84 3 12 221 29 224 5.38e-63 209
MS.gene006573.t1 MTR_8g105780 42.537 268 146 2 12 278 22 282 7.99e-63 209
MS.gene006573.t1 MTR_2g100680 43.602 211 108 2 12 222 31 230 2.72e-54 189
MS.gene006573.t1 MTR_8g083100 45.498 211 103 3 13 223 23 221 5.54e-54 189
MS.gene006573.t1 MTR_6g043060 42.986 221 114 3 2 222 12 220 6.03e-54 184
MS.gene006573.t1 MTR_8g087540 44.865 185 94 2 10 193 10 187 5.63e-52 181
MS.gene006573.t1 MTR_2g100670 46.486 185 89 1 12 196 33 207 3.54e-51 176
MS.gene006573.t1 MTR_6g086805 44.811 212 105 6 7 218 6 205 1.02e-50 176
MS.gene006573.t1 MTR_2g100670 46.486 185 89 1 12 196 141 315 3.63e-50 177
MS.gene006573.t1 MTR_2g017890 39.683 189 103 2 10 194 13 194 1.73e-44 160
MS.gene006573.t1 MTR_5g010680 65.217 92 32 0 12 103 24 115 1.20e-39 147
MS.gene006573.t1 MTR_3g101870 42.640 197 64 4 9 156 21 217 2.13e-39 147
MS.gene006573.t1 MTR_0020s0020 65.217 92 32 0 12 103 33 124 9.15e-38 141
MS.gene006573.t1 MTR_1g061170 35.685 241 128 6 72 309 14 230 4.29e-37 140
MS.gene006573.t1 MTR_5g017470 62.366 93 35 0 12 104 9 101 8.16e-37 137
MS.gene006573.t1 MTR_1g100777 60.870 92 36 0 12 103 18 109 6.80e-36 135
MS.gene006573.t1 MTR_5g089170 61.290 93 36 0 12 104 25 117 2.49e-34 130
MS.gene006573.t1 MTR_7g091370 42.484 153 77 2 12 157 22 170 2.83e-31 120
MS.gene006573.t1 MTR_4g088900 54.639 97 40 1 12 104 33 129 1.55e-30 118
MS.gene006573.t1 MTR_5g047190 48.936 94 41 2 20 106 1 94 1.85e-21 89.4
MS.gene006573.t1 MTR_6g044340 28.910 211 129 6 58 267 8 198 4.08e-18 84.7
MS.gene006573.t1 MTR_4g045757 58.333 60 25 0 10 69 12 71 9.69e-18 78.2
MS.gene006573.t1 MTR_2g043940 32.593 135 70 3 69 193 17 140 4.04e-14 74.3
MS.gene006573.t1 MTR_1g061170 30.909 165 95 4 148 309 1 149 1.72e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006573.t1 AT5G16820 48.491 497 196 11 10 489 25 478 1.62e-136 403
MS.gene006573.t1 AT5G16820 48.491 497 196 11 10 489 25 478 1.62e-136 403
MS.gene006573.t1 AT3G02990 48.441 481 211 9 10 489 21 465 1.97e-131 389
MS.gene006573.t1 AT1G32330 44.177 498 219 14 3 489 28 477 2.10e-116 351
MS.gene006573.t1 AT1G32330 44.177 498 219 14 3 489 28 477 3.08e-116 351
MS.gene006573.t1 AT4G17750 43.340 503 211 16 6 489 46 493 4.97e-111 338
MS.gene006573.t1 AT2G26150 58.095 210 80 2 11 218 43 246 1.78e-80 254
MS.gene006573.t1 AT2G26150 58.095 210 80 2 11 218 43 246 1.78e-80 254
MS.gene006573.t1 AT3G22830 56.731 208 89 1 11 217 60 267 3.73e-74 240
MS.gene006573.t1 AT1G67970 44.366 284 131 8 10 286 17 280 8.49e-64 212
MS.gene006573.t1 AT5G03720 44.351 239 112 5 10 243 53 275 5.26e-61 206
MS.gene006573.t1 AT5G03720 44.351 239 112 5 10 243 53 275 5.26e-61 206
MS.gene006573.t1 AT3G51910 44.700 217 95 3 4 216 21 216 1.66e-57 192
MS.gene006573.t1 AT4G13980 44.928 207 101 3 13 218 24 218 5.86e-56 194
MS.gene006573.t1 AT3G24520 42.925 212 90 4 9 218 14 196 4.30e-52 179
MS.gene006573.t1 AT5G43840 39.834 241 102 6 11 244 16 220 1.85e-50 174
MS.gene006573.t1 AT4G18880 37.134 307 172 6 4 308 7 294 2.09e-48 172
MS.gene006573.t1 AT5G54070 41.538 195 99 1 9 203 68 247 5.88e-48 169
MS.gene006573.t1 AT3G63350 44.103 195 97 2 12 205 28 211 2.15e-47 166
MS.gene006573.t1 AT2G26150 56.376 149 57 2 72 218 49 191 4.36e-47 165
MS.gene006573.t1 AT2G26150 56.376 149 57 2 72 218 49 191 4.36e-47 165
MS.gene006573.t1 AT5G45710 40.889 225 111 5 10 232 11 215 7.68e-47 166
MS.gene006573.t1 AT5G45710 40.889 225 111 5 10 232 11 215 7.68e-47 166
MS.gene006573.t1 AT1G46264 41.624 197 74 3 1 156 23 219 9.57e-42 152
MS.gene006573.t1 AT5G62020 61.702 94 36 0 12 105 23 116 5.38e-37 138
MS.gene006573.t1 AT4G36990 52.672 131 48 2 1 129 1 119 1.25e-36 137
MS.gene006573.t1 AT4G11660 59.783 92 37 0 12 103 59 150 1.05e-34 134
MS.gene006573.t1 AT2G41690 55.000 100 45 0 11 110 39 138 7.66e-33 125
MS.gene006573.t1 AT4G18870 39.773 88 53 0 12 99 146 233 7.61e-19 87.4
MS.gene006573.t1 AT4G18870 43.820 89 45 2 13 99 14 99 1.61e-18 86.3
MS.gene006573.t1 AT1G77570 37.778 90 53 2 13 99 27 116 2.11e-15 73.9

Find 112 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAGGTTCCACCTATTTC+TGG 0.099338 4.1:-26940933 MS.gene006573:CDS
AGTTCATCCACCCAAGTTTC+TGG 0.190167 4.1:-26940966 MS.gene006573:CDS
AGCACACCAAGACAATATTT+TGG 0.232538 4.1:-26940781 MS.gene006573:CDS
CAGGAATCAACGATGAATTC+TGG 0.282845 4.1:-26940578 MS.gene006573:CDS
TTTAGGCCAAGCCCACTTAC+TGG 0.290833 4.1:-26940546 MS.gene006573:CDS
TGGTGTGCTGTTGTAAATTC+TGG 0.291113 4.1:+26940795 None:intergenic
GGATGTGATATCAGCTGTTT+TGG 0.314761 4.1:-26940664 MS.gene006573:CDS
TTGTTGTGTTTGAAATATTT+AGG 0.323777 4.1:+26942380 None:intergenic
AAAGGTAGTTCTCTAGGATA+TGG 0.328048 4.1:-26940507 MS.gene006573:CDS
AAGAGGATTATGTGGATTCT+TGG 0.333992 4.1:+26941154 None:intergenic
CAACCGAATCGATCGTTTCA+TGG 0.334490 4.1:-26942463 MS.gene006573:CDS
TTTGGATGGGACACGGTCTT+TGG 0.334831 4.1:-26940646 MS.gene006573:CDS
ATACATGACTGCCCAGAAAT+AGG 0.338814 4.1:+26940921 None:intergenic
AACTGTTCATTTGCAACATC+TGG 0.343307 4.1:+26941072 None:intergenic
CTATTGGTCATACAACTTAC+AGG 0.350342 4.1:+26940879 None:intergenic
TGTGTGGAAGTGGGTAAGTT+TGG 0.353975 4.1:-26941464 MS.gene006573:CDS
CTGAAGGAACATCATCAATA+AGG 0.355963 4.1:+26941009 None:intergenic
TCTTTGCTAATGACAATTCC+TGG 0.358563 4.1:+26940472 None:intergenic
CTGAGGTTCCACCTATTTCT+GGG 0.367826 4.1:-26940932 MS.gene006573:CDS
TTTGCAGCCTCATTAATAGA+AGG 0.376695 4.1:+26941107 None:intergenic
CATGAATAGTCCGGGCTTCA+TGG 0.377627 4.1:-26941270 MS.gene006573:CDS
CAGAATTCATCGTTGATTCC+TGG 0.379940 4.1:+26940579 None:intergenic
GGAGACACAGACGAAGTTAA+AGG 0.386040 4.1:-26940525 MS.gene006573:CDS
AATCCACATAATCCTCTTGA+TGG 0.386726 4.1:-26941149 MS.gene006573:CDS
CCTAAGCAAAACCTACGATA+TGG 0.391487 4.1:-26942498 MS.gene006573:CDS
ATATTTAGGCAAGATGTGTT+TGG 0.394875 4.1:+26942394 None:intergenic
GTGTCTCCAGTAAGTGGGCT+TGG 0.409905 4.1:+26940540 None:intergenic
CGGTGAGAATGATGATGAAT+TGG 0.410275 4.1:-26940685 MS.gene006573:CDS
CATGAAGCCCGGACTATTCA+TGG 0.411920 4.1:+26941271 None:intergenic
GATGAACTACTGCTATCTAA+TGG 0.419987 4.1:+26940981 None:intergenic
ATTTGTTAAAGAATATCAAT+AGG 0.421836 4.1:-26941556 MS.gene006573:CDS
TGAGCGAATTCCCAGCGGTC+GGG 0.422148 4.1:+26941605 None:intergenic
ATCGTTGATTCCTGGAAGCT+TGG 0.423687 4.1:+26940587 None:intergenic
GTGTGGAAGTGGGTAAGTTT+GGG 0.436120 4.1:-26941463 MS.gene006573:CDS
AACCGAATCGATCGTTTCAT+GGG 0.436953 4.1:-26942462 MS.gene006573:CDS
CAAGATGTGTTTGGAGAAAT+CGG 0.437903 4.1:+26942403 None:intergenic
GTTGCCCAACATTTACCAAT+TGG 0.441846 4.1:+26941348 None:intergenic
AATGAAAATTCCAAGCTTCC+AGG 0.444171 4.1:-26940597 MS.gene006573:CDS
GAATGCATCAAGGTTCTTGA+TGG 0.448987 4.1:+26941031 None:intergenic
GATAACCAATTGGTAAATGT+TGG 0.452224 4.1:-26941353 MS.gene006573:CDS
CGGAAATCTACTCATGCAAA+TGG 0.457161 4.1:-26941533 MS.gene006573:CDS
AGCAAAGGCCATGAATAGTC+CGG 0.460979 4.1:-26941279 MS.gene006573:CDS
GGATCGCGAATTATGACAGC+TGG 0.461443 4.1:-26942548 None:intergenic
GAAGATTCAACAAGAAAAGA+TGG 0.461849 4.1:-26940451 MS.gene006573:CDS
AAAGAGGCGGCTCCAGGGGC+AGG 0.463211 4.1:-26941195 MS.gene006573:CDS
ATAACCAATTGGTAAATGTT+GGG 0.467159 4.1:-26941352 MS.gene006573:CDS
AAAGTGTTACACCAGAAACT+TGG 0.470921 4.1:+26940955 None:intergenic
AACTATCTTCATCCTGCCCC+TGG 0.473972 4.1:+26941183 None:intergenic
AGGAATCAACGATGAATTCT+GGG 0.476706 4.1:-26940577 MS.gene006573:CDS
TGTTGGGTCATGTGTGGAAG+TGG 0.480175 4.1:-26941474 MS.gene006573:CDS
AGCCGCAGAACCCACCTGTT+GGG 0.484089 4.1:-26941490 MS.gene006573:CDS
GAAAGGTTGACCCCGACCGC+TGG 0.485865 4.1:-26941616 MS.gene006573:CDS
AAGAGGTTGAAAGACTGAAA+AGG 0.488568 4.1:-26941433 MS.gene006573:CDS
TATTTCTGGGCAGTCATGTA+TGG 0.490052 4.1:-26940919 MS.gene006573:CDS
GTTCAATTGTCTGACGAAGC+TGG 0.491130 4.1:+26942352 None:intergenic
AGAGGTTGAAAGACTGAAAA+GGG 0.491243 4.1:-26941432 MS.gene006573:CDS
GACTATTCATGGCCTTTGCT+AGG 0.492617 4.1:+26941282 None:intergenic
GAGCGAATTCCCAGCGGTCG+GGG 0.492924 4.1:+26941606 None:intergenic
ACAATCGACTGATAACCAAT+TGG 0.498524 4.1:-26941363 MS.gene006573:CDS
ACCCCATGAAACGATCGATT+CGG 0.504242 4.1:+26942460 None:intergenic
ACTTCCACACATGACCCAAC+AGG 0.505692 4.1:+26941476 None:intergenic
TCGTCTGTGTCTCCAGTAAG+TGG 0.508093 4.1:+26940534 None:intergenic
GAAGTTAAAGGTAGTTCTCT+AGG 0.508241 4.1:-26940513 MS.gene006573:CDS
TTCTGGTGTAACACTTTCTG+AGG 0.510063 4.1:-26940949 MS.gene006573:CDS
CAAAATCCAAAATATTGTCT+TGG 0.518046 4.1:+26940775 None:intergenic
GTACCAGCCTTCTATTAATG+AGG 0.518867 4.1:-26941114 MS.gene006573:CDS
TTGTACCTCAGACATGCTAT+TGG 0.521476 4.1:+26940863 None:intergenic
GTTCCATCTGTTGAGTAAGA+TGG 0.529932 4.1:+26940409 None:intergenic
CGCTGGGAATTCGCTCATGA+AGG 0.530003 4.1:-26941599 MS.gene006573:CDS
CAGCCTCATTAATAGAAGGC+TGG 0.531526 4.1:+26941111 None:intergenic
CGTCCATCAAGAGGATTATG+TGG 0.541079 4.1:+26941146 None:intergenic
CATCAATAAGGAATGCATCA+AGG 0.541216 4.1:+26941021 None:intergenic
AAAGGTTGACCCCGACCGCT+GGG 0.545860 4.1:-26941615 MS.gene006573:CDS
GAAAATTGAGCCATGAAGCC+CGG 0.546699 4.1:+26941260 None:intergenic
ACAGATGGAACTTCTTGCAT+CGG 0.548170 4.1:-26940397 MS.gene006573:CDS
AAGCCGCAGAACCCACCTGT+TGG 0.557194 4.1:-26941491 MS.gene006573:CDS
TAAGTTTGGGCTTGATGAAG+AGG 0.559114 4.1:-26941450 MS.gene006573:CDS
GCAAAGGCCATGAATAGTCC+GGG 0.565582 4.1:-26941278 MS.gene006573:CDS
AATGAACAGTTCTGCAAGGG+TGG 0.569475 4.1:-26941060 MS.gene006573:CDS
ATGAGCGAATTCCCAGCGGT+CGG 0.572798 4.1:+26941604 None:intergenic
GATGATGTCGTTCCTAGCAA+AGG 0.573149 4.1:-26941294 MS.gene006573:CDS
ATATGGTTTGACTAAAGACC+AGG 0.574236 4.1:-26940490 MS.gene006573:CDS
GGACCATCTTACTCAACAGA+TGG 0.577724 4.1:-26940412 MS.gene006573:CDS
AAGTGTTACACCAGAAACTT+GGG 0.578836 4.1:+26940956 None:intergenic
CTATCTAATGGAATGGCTGA+AGG 0.583304 4.1:+26940993 None:intergenic
TCCACACATGACCCAACAGG+TGG 0.591825 4.1:+26941479 None:intergenic
AGGATTATGTGGATTCTTGG+TGG 0.592009 4.1:+26941157 None:intergenic
GACCCAACAGGTGGGTTCTG+CGG 0.593008 4.1:+26941488 None:intergenic
AGTAATAGACATGTTACCGC+TGG 0.593286 4.1:-26941221 MS.gene006573:CDS
CGTCTGTGTCTCCAGTAAGT+GGG 0.594211 4.1:+26940535 None:intergenic
ACACAATCAGTCAACACAGC+AGG 0.595480 4.1:+26940834 None:intergenic
TGTAAATTCTGGAAATTCAG+CGG 0.596634 4.1:+26940806 None:intergenic
TGCATCAAGGTTCTTGATGG+AGG 0.607948 4.1:+26941034 None:intergenic
AGAGTCATCAACCATATCGT+AGG 0.608336 4.1:+26942487 None:intergenic
TGGGCAACGTGTGCAAGTGA+TGG 0.618938 4.1:-26941333 MS.gene006573:CDS
TTAACAACACGTCCATCAAG+AGG 0.623081 4.1:+26941137 None:intergenic
ACTACTGCTATCTAATGGAA+TGG 0.639316 4.1:+26940986 None:intergenic
CCCACCTGTTGGGTCATGTG+TGG 0.640259 4.1:-26941480 MS.gene006573:CDS
GATCGCGAATTATGACAGCT+GGG 0.642062 4.1:-26942547 None:intergenic
TGTTAAAGAATATCAATAGG+CGG 0.647648 4.1:-26941553 MS.gene006573:CDS
TATGACCAATAGCATGTCTG+AGG 0.654015 4.1:-26940868 MS.gene006573:CDS
TGCAAATGAACAGTTCTGCA+AGG 0.655638 4.1:-26941064 MS.gene006573:CDS
CATGACTGCCCAGAAATAGG+TGG 0.662832 4.1:+26940924 None:intergenic
GTTGGGTCATGTGTGGAAGT+GGG 0.670827 4.1:-26941473 MS.gene006573:CDS
ACCGAATCGATCGTTTCATG+GGG 0.677166 4.1:-26942461 MS.gene006573:CDS
GCAAATGAACAGTTCTGCAA+GGG 0.681864 4.1:-26941063 MS.gene006573:CDS
GATGGATAAGATACAGCACA+TGG 0.683473 4.1:-26940433 MS.gene006573:CDS
CCACACATGACCCAACAGGT+GGG 0.694523 4.1:+26941480 None:intergenic
TGTTACACCAGAAACTTGGG+TGG 0.706092 4.1:+26940959 None:intergenic
GGTGGAATTGAAAACGACGA+AGG 0.715764 4.1:+26942424 None:intergenic
GATGTGTTTGGAGAAATCGG+TGG 0.725221 4.1:+26942406 None:intergenic
CTTCATGAGCGAATTCCCAG+CGG 0.750748 4.1:+26941600 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCTTAGAAAATATTCAT+CGG + chr4.1:26940911-26940930 None:intergenic 15.0%
!!! ATTTGTTAAAGAATATCAAT+AGG - chr4.1:26941357-26941376 MS.gene006573:CDS 15.0%
!!! TTTTCAGATTTTCTTAATTA+GGG - chr4.1:26940608-26940627 MS.gene006573:CDS 15.0%
!! ATGTTATTAAAAAAGCAAGA+AGG + chr4.1:26941174-26941193 None:intergenic 20.0%
!! CTAATTAAGAAAATCTGAAA+AGG + chr4.1:26940609-26940628 None:intergenic 20.0%
!! TAACATACATTTAATAATGC+TGG - chr4.1:26941188-26941207 MS.gene006573:CDS 20.0%
!!! CTTTTCAGATTTTCTTAATT+AGG - chr4.1:26940607-26940626 MS.gene006573:CDS 20.0%
!!! GATGAATATTTTCTAAGAAT+TGG - chr4.1:26940910-26940929 MS.gene006573:CDS 20.0%
!!! TTAAGTAGTAATAGGATTTT+AGG - chr4.1:26941047-26941066 MS.gene006573:CDS 20.0%
!!! TTGTTGTGTTTGAAATATTT+AGG + chr4.1:26940536-26940555 None:intergenic 20.0%
! ATAACCAATTGGTAAATGTT+GGG - chr4.1:26941561-26941580 MS.gene006573:CDS 25.0%
! CAAAATCCAAAATATTGTCT+TGG + chr4.1:26942141-26942160 None:intergenic 25.0%
! GTAATTAGAGAAGTTACATT+CGG - chr4.1:26940795-26940814 MS.gene006573:CDS 25.0%
! GTTCAGATCATATTAACTTA+TGG - chr4.1:26940651-26940670 MS.gene006573:CDS 25.0%
! TAATTAGAGAAGTTACATTC+GGG - chr4.1:26940796-26940815 MS.gene006573:CDS 25.0%
! TGTTAAAGAATATCAATAGG+CGG - chr4.1:26941360-26941379 MS.gene006573:CDS 25.0%
!! AATCCTGATCAAAATTTTGT+GGG - chr4.1:26942199-26942218 MS.gene006573:intron 25.0%
!! CTTAATTGTAATTTGGTGAT+TGG - chr4.1:26940756-26940775 MS.gene006573:CDS 25.0%
!! TTATGGACGAAGATTTTATT+AGG - chr4.1:26940668-26940687 MS.gene006573:CDS 25.0%
!! TTGTATACTTTTAATCATGC+TGG - chr4.1:26941223-26941242 MS.gene006573:CDS 25.0%
!!! AATTCTGGGAACAATTTTTT+AGG - chr4.1:26942350-26942369 MS.gene006573:CDS 25.0%
!!! AGTAATAGGATTTTAGGTTA+TGG - chr4.1:26941053-26941072 MS.gene006573:CDS 25.0%
!!! ATCTTTTTTATTCACAAGCA+GGG - chr4.1:26941269-26941288 MS.gene006573:CDS 25.0%
!!! ATTTTCTAAGAATTGGATGT+AGG - chr4.1:26940917-26940936 MS.gene006573:CDS 25.0%
!!! CAAGACAATATTTTGGATTT+TGG - chr4.1:26942139-26942158 MS.gene006573:intron 25.0%
!!! TATCTTTTTTATTCACAAGC+AGG - chr4.1:26941268-26941287 MS.gene006573:CDS 25.0%
!!! TTTTAGGTTATGGTTAAGAT+AGG - chr4.1:26941063-26941082 MS.gene006573:CDS 25.0%
ATATTTAGGCAAGATGTGTT+TGG + chr4.1:26940522-26940541 None:intergenic 30.0%
ATTAAAAAAGCAAGAAGGAG+AGG + chr4.1:26941169-26941188 None:intergenic 30.0%
GAAGATTCAACAAGAAAAGA+TGG - chr4.1:26942462-26942481 MS.gene006573:CDS 30.0%
GACTGCAATTAAGTAGTAAT+AGG - chr4.1:26941039-26941058 MS.gene006573:CDS 30.0%
GATAACCAATTGGTAAATGT+TGG - chr4.1:26941560-26941579 MS.gene006573:CDS 30.0%
TAACTAATGAATCCCCAATA+GGG + chr4.1:26941091-26941110 None:intergenic 30.0%
TGTTAGAACTGAATTCTACA+CGG - chr4.1:26941003-26941022 MS.gene006573:CDS 30.0%
! ATCCTGATCAAAATTTTGTG+GGG - chr4.1:26942200-26942219 MS.gene006573:intron 30.0%
! GAATCCTGATCAAAATTTTG+TGG - chr4.1:26942198-26942217 MS.gene006573:intron 30.0%
! TGTAAATTCTGGAAATTCAG+CGG + chr4.1:26942110-26942129 None:intergenic 30.0%
AACTGTTCATTTGCAACATC+TGG + chr4.1:26941844-26941863 None:intergenic 35.0%
AAGAGGATTATGTGGATTCT+TGG + chr4.1:26941762-26941781 None:intergenic 35.0%
AAGAGGTTGAAAGACTGAAA+AGG - chr4.1:26941480-26941499 MS.gene006573:CDS 35.0%
AAGTGTTACACCAGAAACTT+GGG + chr4.1:26941960-26941979 None:intergenic 35.0%
AATCCACATAATCCTCTTGA+TGG - chr4.1:26941764-26941783 MS.gene006573:intron 35.0%
AATGAAAATTCCAAGCTTCC+AGG - chr4.1:26942316-26942335 MS.gene006573:intron 35.0%
ACAATCGACTGATAACCAAT+TGG - chr4.1:26941550-26941569 MS.gene006573:CDS 35.0%
ACTACTGCTATCTAATGGAA+TGG + chr4.1:26941930-26941949 None:intergenic 35.0%
AGAGGTTGAAAGACTGAAAA+GGG - chr4.1:26941481-26941500 MS.gene006573:CDS 35.0%
AGGAATCAACGATGAATTCT+GGG - chr4.1:26942336-26942355 MS.gene006573:intron 35.0%
ATATGGTTTGACTAAAGACC+AGG - chr4.1:26942423-26942442 MS.gene006573:CDS 35.0%
CAAGATGTGTTTGGAGAAAT+CGG + chr4.1:26940513-26940532 None:intergenic 35.0%
CATCAATAAGGAATGCATCA+AGG + chr4.1:26941895-26941914 None:intergenic 35.0%
CATTTGCAAACTGCAAAAAG+TGG + chr4.1:26940985-26941004 None:intergenic 35.0%
CTAACTAATGAATCCCCAAT+AGG + chr4.1:26941092-26941111 None:intergenic 35.0%
CTATTGGTCATACAACTTAC+AGG + chr4.1:26942037-26942056 None:intergenic 35.0%
CTGAAGGAACATCATCAATA+AGG + chr4.1:26941907-26941926 None:intergenic 35.0%
GAACGCGCTTAATTGTAATT+TGG - chr4.1:26940749-26940768 MS.gene006573:CDS 35.0%
GAAGTTAAAGGTAGTTCTCT+AGG - chr4.1:26942400-26942419 MS.gene006573:CDS 35.0%
GATGAACTACTGCTATCTAA+TGG + chr4.1:26941935-26941954 None:intergenic 35.0%
GGTAAAGTAAGAACATACGT+AGG + chr4.1:26940588-26940607 None:intergenic 35.0%
GTTAAGATAGGTACCCTATT+GGG - chr4.1:26941075-26941094 MS.gene006573:CDS 35.0%
TAACATTTCCAAGAGTTTCG+TGG - chr4.1:26940940-26940959 MS.gene006573:CDS 35.0%
TCTTTGCTAATGACAATTCC+TGG + chr4.1:26942444-26942463 None:intergenic 35.0%
TTAAGATAGGTACCCTATTG+GGG - chr4.1:26941076-26941095 MS.gene006573:CDS 35.0%
TTTGCAGCCTCATTAATAGA+AGG + chr4.1:26941809-26941828 None:intergenic 35.0%
! AAAGTGTTACACCAGAAACT+TGG + chr4.1:26941961-26941980 None:intergenic 35.0%
! AGCACACCAAGACAATATTT+TGG - chr4.1:26942132-26942151 MS.gene006573:intron 35.0%
! CGCTCATGAAGGATTTTTAA+GGG - chr4.1:26941325-26941344 MS.gene006573:CDS 35.0%
! TCGCTCATGAAGGATTTTTA+AGG - chr4.1:26941324-26941343 MS.gene006573:CDS 35.0%
! TGTAATTTGGTGATTGGAGA+GGG - chr4.1:26940762-26940781 MS.gene006573:CDS 35.0%
! TTGTAATTTGGTGATTGGAG+AGG - chr4.1:26940761-26940780 MS.gene006573:CDS 35.0%
!! AAAGGTAGTTCTCTAGGATA+TGG - chr4.1:26942406-26942425 MS.gene006573:CDS 35.0%
!! GCTCATGAAGGATTTTTAAG+GGG - chr4.1:26941326-26941345 MS.gene006573:CDS 35.0%
!! TGATATCAGCTGTTTTGGAT+GGG - chr4.1:26942254-26942273 MS.gene006573:intron 35.0%
!! TTGGATTTTGGTGAAGTTCA+AGG - chr4.1:26942151-26942170 MS.gene006573:intron 35.0%
AACCGAATCGATCGTTTCAT+GGG - chr4.1:26940451-26940470 MS.gene006573:CDS 40.0%
ACAGATGGAACTTCTTGCAT+CGG - chr4.1:26942516-26942535 MS.gene006573:CDS 40.0%
ACAGTAACAGTAACACTAGC+AGG + chr4.1:26940861-26940880 None:intergenic 40.0%
AGAGTCATCAACCATATCGT+AGG + chr4.1:26940429-26940448 None:intergenic 40.0%
AGGATTATGTGGATTCTTGG+TGG + chr4.1:26941759-26941778 None:intergenic 40.0%
AGTAATAGACATGTTACCGC+TGG - chr4.1:26941692-26941711 MS.gene006573:intron 40.0%
ATACATGACTGCCCAGAAAT+AGG + chr4.1:26941995-26942014 None:intergenic 40.0%
ATGTTACCGCTGGTAAAAAG+AGG - chr4.1:26941702-26941721 MS.gene006573:intron 40.0%
ATTTCCAAGAGTTTCGTGGA+AGG - chr4.1:26940944-26940963 MS.gene006573:CDS 40.0%
CAAAGTCAAGATACTTGTCC+TGG - chr4.1:26941124-26941143 MS.gene006573:CDS 40.0%
CAGGAATCAACGATGAATTC+TGG - chr4.1:26942335-26942354 MS.gene006573:intron 40.0%
CCTAAGCAAAACCTACGATA+TGG - chr4.1:26940415-26940434 MS.gene006573:CDS 40.0%
CGGAAATCTACTCATGCAAA+TGG - chr4.1:26941380-26941399 MS.gene006573:CDS 40.0%
CTATCTAATGGAATGGCTGA+AGG + chr4.1:26941923-26941942 None:intergenic 40.0%
GAATGCATCAAGGTTCTTGA+TGG + chr4.1:26941885-26941904 None:intergenic 40.0%
GATGGATAAGATACAGCACA+TGG - chr4.1:26942480-26942499 MS.gene006573:CDS 40.0%
GCAAATGAACAGTTCTGCAA+GGG - chr4.1:26941850-26941869 MS.gene006573:intron 40.0%
GGTTAAGATAGGTACCCTAT+TGG - chr4.1:26941074-26941093 MS.gene006573:CDS 40.0%
GTACCAGCCTTCTATTAATG+AGG - chr4.1:26941799-26941818 MS.gene006573:intron 40.0%
GTTACATTCGGGATGCATTT+CGG - chr4.1:26940807-26940826 MS.gene006573:CDS 40.0%
GTTCCATCTGTTGAGTAAGA+TGG + chr4.1:26942507-26942526 None:intergenic 40.0%
GTTGCCCAACATTTACCAAT+TGG + chr4.1:26941568-26941587 None:intergenic 40.0%
TAAGTTTGGGCTTGATGAAG+AGG - chr4.1:26941463-26941482 MS.gene006573:CDS 40.0%
TATGACCAATAGCATGTCTG+AGG - chr4.1:26942045-26942064 MS.gene006573:intron 40.0%
TGCAAATGAACAGTTCTGCA+AGG - chr4.1:26941849-26941868 MS.gene006573:intron 40.0%
TTAACAACACGTCCATCAAG+AGG + chr4.1:26941779-26941798 None:intergenic 40.0%
TTCTGGTGTAACACTTTCTG+AGG - chr4.1:26941964-26941983 MS.gene006573:intron 40.0%
TTGTACCTCAGACATGCTAT+TGG + chr4.1:26942053-26942072 None:intergenic 40.0%
! CAAAATTTTGTGGGGTCTGT+CGG - chr4.1:26942208-26942227 MS.gene006573:intron 40.0%
! CCATATCGTAGGTTTTGCTT+AGG + chr4.1:26940418-26940437 None:intergenic 40.0%
! CGGTGAGAATGATGATGAAT+TGG - chr4.1:26942228-26942247 MS.gene006573:intron 40.0%
! GGATGTGATATCAGCTGTTT+TGG - chr4.1:26942249-26942268 MS.gene006573:intron 40.0%
! TCGTAGGTTTTGCTTAGGAA+CGG + chr4.1:26940413-26940432 None:intergenic 40.0%
! TGCTAGTGTTACTGTTACTG+TGG - chr4.1:26940860-26940879 MS.gene006573:CDS 40.0%
! TGGTGTGCTGTTGTAAATTC+TGG + chr4.1:26942121-26942140 None:intergenic 40.0%
! TTTTGGTGAAGTTCAAGGAC+TGG - chr4.1:26942156-26942175 MS.gene006573:intron 40.0%
!! CAGAATTCATCGTTGATTCC+TGG + chr4.1:26942337-26942356 None:intergenic 40.0%
!! GACCCCACAAAATTTTGATC+AGG + chr4.1:26942205-26942224 None:intergenic 40.0%
!! GTGATATCAGCTGTTTTGGA+TGG - chr4.1:26942253-26942272 MS.gene006573:intron 40.0%
!! TATTTCTGGGCAGTCATGTA+TGG - chr4.1:26941994-26942013 MS.gene006573:intron 40.0%
!!! TCACAAGCAGGGTTTTAGAA+AGG - chr4.1:26941280-26941299 MS.gene006573:CDS 40.0%
AATGAACAGTTCTGCAAGGG+TGG - chr4.1:26941853-26941872 MS.gene006573:intron 45.0%
ACACAATCAGTCAACACAGC+AGG + chr4.1:26942082-26942101 None:intergenic 45.0%
ACCCCATGAAACGATCGATT+CGG + chr4.1:26940456-26940475 None:intergenic 45.0%
ACCGAATCGATCGTTTCATG+GGG - chr4.1:26940452-26940471 MS.gene006573:CDS 45.0%
AGCAAAGGCCATGAATAGTC+CGG - chr4.1:26941634-26941653 MS.gene006573:intron 45.0%
AGTTCATCCACCCAAGTTTC+TGG - chr4.1:26941947-26941966 MS.gene006573:intron 45.0%
ATATTCATCGGTACACCGAG+CGG + chr4.1:26940899-26940918 None:intergenic 45.0%
CAACCGAATCGATCGTTTCA+TGG - chr4.1:26940450-26940469 MS.gene006573:CDS 45.0%
CAGCCTCATTAATAGAAGGC+TGG + chr4.1:26941805-26941824 None:intergenic 45.0%
CATTCCTTCCACGAAACTCT+TGG + chr4.1:26940951-26940970 None:intergenic 45.0%
CGTCCATCAAGAGGATTATG+TGG + chr4.1:26941770-26941789 None:intergenic 45.0%
GAAAATTGAGCCATGAAGCC+CGG + chr4.1:26941656-26941675 None:intergenic 45.0%
GACTATTCATGGCCTTTGCT+AGG + chr4.1:26941634-26941653 None:intergenic 45.0%
GATGATGTCGTTCCTAGCAA+AGG - chr4.1:26941619-26941638 MS.gene006573:CDS 45.0%
GATGTGTTTGGAGAAATCGG+TGG + chr4.1:26940510-26940529 None:intergenic 45.0%
GGACCATCTTACTCAACAGA+TGG - chr4.1:26942501-26942520 MS.gene006573:CDS 45.0%
GGAGACACAGACGAAGTTAA+AGG - chr4.1:26942388-26942407 MS.gene006573:CDS 45.0%
GGTGGAATTGAAAACGACGA+AGG + chr4.1:26940492-26940511 None:intergenic 45.0%
GTGTGGAAGTGGGTAAGTTT+GGG - chr4.1:26941450-26941469 MS.gene006573:CDS 45.0%
GTTCAATTGTCTGACGAAGC+TGG + chr4.1:26940564-26940583 None:intergenic 45.0%
TGCAAACTGCAAAAAGTGGC+TGG + chr4.1:26940981-26941000 None:intergenic 45.0%
TGCATCAAGGTTCTTGATGG+AGG + chr4.1:26941882-26941901 None:intergenic 45.0%
TGTGTGGAAGTGGGTAAGTT+TGG - chr4.1:26941449-26941468 MS.gene006573:CDS 45.0%
TGTTACACCAGAAACTTGGG+TGG + chr4.1:26941957-26941976 None:intergenic 45.0%
TTACCGCTGGTAAAAAGAGG+CGG - chr4.1:26941705-26941724 MS.gene006573:intron 45.0%
! CTGAGGTTCCACCTATTTCT+GGG - chr4.1:26941981-26942000 MS.gene006573:intron 45.0%
! TAGGTTTTGCTTAGGAACGG+TGG + chr4.1:26940410-26940429 None:intergenic 45.0%
! TCTGAGGTTCCACCTATTTC+TGG - chr4.1:26941980-26941999 MS.gene006573:intron 45.0%
!! ATCGTTGATTCCTGGAAGCT+TGG + chr4.1:26942329-26942348 None:intergenic 45.0%
AACTATCTTCATCCTGCCCC+TGG + chr4.1:26941733-26941752 None:intergenic 50.0%
ACTTCCACACATGACCCAAC+AGG + chr4.1:26941440-26941459 None:intergenic 50.0%
AGATACTTGTCCTGGTGCTC+TGG - chr4.1:26941132-26941151 MS.gene006573:CDS 50.0%
CATGAAGCCCGGACTATTCA+TGG + chr4.1:26941645-26941664 None:intergenic 50.0%
CATGAATAGTCCGGGCTTCA+TGG - chr4.1:26941643-26941662 MS.gene006573:intron 50.0%
CATGACTGCCCAGAAATAGG+TGG + chr4.1:26941992-26942011 None:intergenic 50.0%
CGTCTGTGTCTCCAGTAAGT+GGG + chr4.1:26942381-26942400 None:intergenic 50.0%
CTTCATGAGCGAATTCCCAG+CGG + chr4.1:26941316-26941335 None:intergenic 50.0%
GCAAAGGCCATGAATAGTCC+GGG - chr4.1:26941635-26941654 MS.gene006573:intron 50.0%
GTTGGGTCATGTGTGGAAGT+GGG - chr4.1:26941440-26941459 MS.gene006573:CDS 50.0%
TCGTCTGTGTCTCCAGTAAG+TGG + chr4.1:26942382-26942401 None:intergenic 50.0%
TGTTGGGTCATGTGTGGAAG+TGG - chr4.1:26941439-26941458 MS.gene006573:CDS 50.0%
TTTAGGCCAAGCCCACTTAC+TGG - chr4.1:26942367-26942386 MS.gene006573:CDS 50.0%
TTTGGATGGGACACGGTCTT+TGG - chr4.1:26942267-26942286 MS.gene006573:intron 50.0%
! AAATTCAGCACCAGAGCACC+AGG + chr4.1:26941145-26941164 None:intergenic 50.0%
!! CAGCTGTTTTGGATGGGACA+CGG - chr4.1:26942260-26942279 MS.gene006573:intron 50.0%
ATGAGCGAATTCCCAGCGGT+CGG + chr4.1:26941312-26941331 None:intergenic 55.0%
CCACACATGACCCAACAGGT+GGG + chr4.1:26941436-26941455 None:intergenic 55.0%
CGCTGGGAATTCGCTCATGA+AGG - chr4.1:26941314-26941333 MS.gene006573:CDS 55.0%
GGTAAAAAGAGGCGGCTCCA+GGG - chr4.1:26941713-26941732 MS.gene006573:intron 55.0%
GTAAAAAGAGGCGGCTCCAG+GGG - chr4.1:26941714-26941733 MS.gene006573:intron 55.0%
TCCACACATGACCCAACAGG+TGG + chr4.1:26941437-26941456 None:intergenic 55.0%
TGGGCAACGTGTGCAAGTGA+TGG - chr4.1:26941580-26941599 MS.gene006573:CDS 55.0%
TGGTAAAAAGAGGCGGCTCC+AGG - chr4.1:26941712-26941731 MS.gene006573:intron 55.0%
! GAGCCGCCTCTTTTTACCAG+CGG + chr4.1:26941711-26941730 None:intergenic 55.0%
! GTGTCTCCAGTAAGTGGGCT+TGG + chr4.1:26942376-26942395 None:intergenic 55.0%
AAAGGTTGACCCCGACCGCT+GGG - chr4.1:26941298-26941317 MS.gene006573:CDS 60.0%
AAGCCGCAGAACCCACCTGT+TGG - chr4.1:26941422-26941441 MS.gene006573:CDS 60.0%
AGCCGCAGAACCCACCTGTT+GGG - chr4.1:26941423-26941442 MS.gene006573:CDS 60.0%
CCCACCTGTTGGGTCATGTG+TGG - chr4.1:26941433-26941452 MS.gene006573:CDS 60.0%
GGTGATTGGAGAGGGAGCTG+TGG - chr4.1:26940770-26940789 MS.gene006573:CDS 60.0%
TGAGCGAATTCCCAGCGGTC+GGG + chr4.1:26941311-26941330 None:intergenic 60.0%
! GACCCAACAGGTGGGTTCTG+CGG + chr4.1:26941428-26941447 None:intergenic 60.0%
CGGTACACCGAGCGGAAGCA+TGG + chr4.1:26940891-26940910 None:intergenic 65.0%
GAAAGGTTGACCCCGACCGC+TGG - chr4.1:26941297-26941316 MS.gene006573:CDS 65.0%
GAGCGAATTCCCAGCGGTCG+GGG + chr4.1:26941310-26941329 None:intergenic 65.0%
! GGTGCTGCCATGCTTCCGCT+CGG - chr4.1:26940881-26940900 MS.gene006573:CDS 65.0%
AAAGAGGCGGCTCCAGGGGC+AGG - chr4.1:26941718-26941737 MS.gene006573:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 26940377 26942558 26940377 ID=MS.gene006573
chr4.1 mRNA 26940377 26942558 26940377 ID=MS.gene006573.t1;Parent=MS.gene006573
chr4.1 exon 26942346 26942558 26942346 ID=MS.gene006573.t1.exon1;Parent=MS.gene006573.t1
chr4.1 CDS 26942346 26942558 26942346 ID=cds.MS.gene006573.t1;Parent=MS.gene006573.t1
chr4.1 exon 26940377 26941645 26940377 ID=MS.gene006573.t1.exon2;Parent=MS.gene006573.t1
chr4.1 CDS 26940377 26941645 26940377 ID=cds.MS.gene006573.t1;Parent=MS.gene006573.t1
Gene Sequence

>MS.gene006573

ATGACAGCTGGGATCGCGAATTATGTATTACCACCGTTCCTAAGCAAAACCTACGATATGGTTGATGACTCTTCAACCGAATCGATCGTTTCATGGGGTAAAAACGACAACACCTTCGTCGTTTTCAATTCCACCGATTTCTCCAAACACATCTTGCCTAAATATTTCAAACACAACAACTTCTCCAGCTTCGTCAGACAATTGAACACCTACGTATGTTCTTACTTTACCTTTTCAGATTTTCTTAATTAGGGTTTTACAGTAATTCATTTTTGTTCAGATCATATTAACTTATGGACGAAGATTTTATTAGGTTTTGTAATTGATAGAATTTATCTATTTATTCATAAATTGCTCAGTGATTATAATTGTGAACGCGCTTAATTGTAATTTGGTGATTGGAGAGGGAGCTGTGGTTGTAATTAGAGAAGTTACATTCGGGATGCATTTCGGATTGTAAATGCAAAGATACAGTTAGCTACCTGCTAGTGTTACTGTTACTGTGGTGCTGCCATGCTTCCGCTCGGTGTACCGATGAATATTTTCTAAGAATTGGATGTAGGTAACATTTCCAAGAGTTTCGTGGAAGGAATGTTTCAAACCAGCCACTTTTTGCAGTTTGCAAATGTTAGAACTGAATTCTACACGGTAAGTAGAATAGTGACTGCAATTAAGTAGTAATAGGATTTTAGGTTATGGTTAAGATAGGTACCCTATTGGGGATTCATTAGTTAGTTAGTTAGTTATCAAAGTCAAGATACTTGTCCTGGTGCTCTGGTGCTGAATTTACCTCTCCTTCTTGCTTTTTTAATAACATACATTTAATAATGCTGGACTATTTGTATATTGTATACTTTTAATCATGCTGGAGTATTTACTCATCATATCAACTATCTTTTTTATTCACAAGCAGGGTTTTAGAAAGGTTGACCCCGACCGCTGGGAATTCGCTCATGAAGGATTTTTAAGGGGTCAAAAACATTTGTTAAAGAATATCAATAGGCGGAAATCTACTCATGCAAATGGTAATAATCAGCAGCTATCTAAGCCGCAGAACCCACCTGTTGGGTCATGTGTGGAAGTGGGTAAGTTTGGGCTTGATGAAGAGGTTGAAAGACTGAAAAGGGACAAGAATGTTCTTATGCAAGAACTTGTTAAGTTAAGACAGCAACAACAATCGACTGATAACCAATTGGTAAATGTTGGGCAACGTGTGCAAGTGATGGAGCAGCGTCAGCAACAGATGATGTCGTTCCTAGCAAAGGCCATGAATAGTCCGGGCTTCATGGCTCAATTTTCACAGCAGCAGAATGAAAGTAATAGACATGTTACCGCTGGTAAAAAGAGGCGGCTCCAGGGGCAGGATGAAGATAGTTTAGCCACCAAGAATCCACATAATCCTCTTGATGGACGTGTTGTTAAGTACCAGCCTTCTATTAATGAGGCTGCAAAAACATTATTTAACCAGATGTTGCAAATGAACAGTTCTGCAAGGGTGGATTCCTCCATCAAGAACCTTGATGCATTCCTTATTGATGATGTTCCTTCAGCCATTCCATTAGATAGCAGTAGTTCATCCACCCAAGTTTCTGGTGTAACACTTTCTGAGGTTCCACCTATTTCTGGGCAGTCATGTATGGCAGTAGAATCTCAATTTCCTGTAAGTTGTATGACCAATAGCATGTCTGAGGTACAATCTTCACCTGCTGTGTTGACTGATTGTGTCAAAACCGCTGAATTTCCAGAATTTACAACAGCACACCAAGACAATATTTTGGATTTTGGTGAAGTTCAAGGACTGGCTACAGAAAGCAGCTTCATGAATCCTGATCAAAATTTTGTGGGGTCTGTCGGTGAGAATGATGATGAATTGGATGTGATATCAGCTGTTTTGGATGGGACACGGTCTTTGGAAGCTGATGCTTTTTCTTCTGATGCGAATGAAAATTCCAAGCTTCCAGGAATCAACGATGAATTCTGGGAACAATTTTTTAGGCCAAGCCCACTTACTGGAGACACAGACGAAGTTAAAGGTAGTTCTCTAGGATATGGTTTGACTAAAGACCAGGAATTGTCATTAGCAAAGAAGATTCAACAAGAAAAGATGGATAAGATACAGCACATGGACCATCTTACTCAACAGATGGAACTTCTTGCATCGGATTCAACATTGTGCATATAA
Protein sequence

>MS.gene006573.t1

MTAGIANYVLPPFLSKTYDMVDDSSTESIVSWGKNDNTFVVFNSTDFSKHILPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFAHEGFLRGQKHLLKNINRRKSTHANGNNQQLSKPQNPPVGSCVEVGKFGLDEEVERLKRDKNVLMQELVKLRQQQQSTDNQLVNVGQRVQVMEQRQQQMMSFLAKAMNSPGFMAQFSQQQNESNRHVTAGKKRRLQGQDEDSLATKNPHNPLDGRVVKYQPSINEAAKTLFNQMLQMNSSARVDSSIKNLDAFLIDDVPSAIPLDSSSSSTQVSGVTLSEVPPISGQSCMAVESQFPVSCMTNSMSEVQSSPAVLTDCVKTAEFPEFTTAHQDNILDFGEVQGLATESSFMNPDQNFVGSVGENDDELDVISAVLDGTRSLEADAFSSDANENSKLPGINDEFWEQFFRPSPLTGDTDEVKGSSLGYGLTKDQELSLAKKIQQEKMDKIQHMDHLTQQMELLASDSTLCI