Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006598.t1 | XP_003607469.1 | 95.2 | 208 | 9 | 1 | 1 | 207 | 1 | 208 | 3.50E-105 | 391 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006598.t1 | F4JIJ7 | 42.9 | 189 | 88 | 8 | 8 | 180 | 2 | 186 | 3.4e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006598.t1 | G7JTT7 | 95.2 | 208 | 9 | 1 | 1 | 207 | 1 | 208 | 2.5e-105 | 391.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene006598.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006598.t1 | MTR_4g078350 | 95.192 | 208 | 9 | 1 | 1 | 207 | 1 | 208 | 1.26e-145 | 404 |
| MS.gene006598.t1 | MTR_2g090480 | 64.000 | 200 | 61 | 4 | 8 | 201 | 9 | 203 | 2.44e-82 | 244 |
| MS.gene006598.t1 | MTR_2g090440 | 64.467 | 197 | 63 | 4 | 8 | 201 | 9 | 201 | 7.13e-82 | 243 |
| MS.gene006598.t1 | MTR_2g090425 | 65.152 | 198 | 59 | 5 | 8 | 199 | 9 | 202 | 3.36e-79 | 240 |
| MS.gene006598.t1 | MTR_2g090500 | 59.236 | 157 | 59 | 3 | 48 | 201 | 60 | 214 | 1.36e-56 | 179 |
| MS.gene006598.t1 | MTR_2g013920 | 46.667 | 180 | 79 | 8 | 7 | 180 | 3 | 171 | 1.28e-40 | 136 |
| MS.gene006598.t1 | MTR_2g090515 | 70.000 | 50 | 12 | 1 | 8 | 57 | 9 | 55 | 1.83e-14 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006598.t1 | AT4G20970 | 42.328 | 189 | 89 | 8 | 8 | 180 | 2 | 186 | 7.18e-32 | 114 |
| MS.gene006598.t1 | AT1G10585 | 28.161 | 174 | 114 | 6 | 11 | 182 | 11 | 175 | 2.31e-12 | 63.2 |
| MS.gene006598.t1 | AT1G10586 | 27.326 | 172 | 114 | 6 | 11 | 180 | 11 | 173 | 1.96e-11 | 60.5 |
Find 43 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCATCTTGTGTTCAATT+TGG | 0.181511 | 4.4:-32235057 | MS.gene006598:CDS |
| ATTTCAAAACATGGTGATTT+TGG | 0.208325 | 4.4:+32235346 | None:intergenic |
| AGAACCTTGGGGTTATAGTT+AGG | 0.280194 | 4.4:+32235807 | None:intergenic |
| TATCTAATTATGGATCAAAT+TGG | 0.283616 | 4.4:-32235939 | None:intergenic |
| AGGGAGAAGAGAGTTGAGTT+TGG | 0.296752 | 4.4:+32235827 | None:intergenic |
| GAGAGTTGAGTTTGGAGTAG+AGG | 0.397503 | 4.4:+32235835 | None:intergenic |
| TGAATTATCTCCAACCTTAG+AGG | 0.408147 | 4.4:+32235204 | None:intergenic |
| GGAATTACCGTTGGCGCTAC+CGG | 0.409114 | 4.4:-32235494 | MS.gene006598:CDS |
| AACCAGCCTTCTTTCAACTT+TGG | 0.410390 | 4.4:+32235887 | None:intergenic |
| GAACCTTGGGGTTATAGTTA+GGG | 0.410715 | 4.4:+32235808 | None:intergenic |
| TGATTTCATAGAAAATGAAT+TGG | 0.413742 | 4.4:+32235272 | None:intergenic |
| AAGGAGTGTCAATTCCTCTA+AGG | 0.421100 | 4.4:-32235218 | MS.gene006598:CDS |
| AAGAAAGAAAGGTTATTAGC+TGG | 0.449807 | 4.4:-32235406 | MS.gene006598:CDS |
| ATCGCCTCATCTACTTGATC+CGG | 0.457881 | 4.4:+32235475 | None:intergenic |
| AGATTGAGAAGGTTTGTGTA+TGG | 0.481420 | 4.4:-32235015 | MS.gene006598:CDS |
| TTGGTTGTATTAGAGCTAGA+AGG | 0.500610 | 4.4:+32235906 | None:intergenic |
| ATGAAGAGAATGTAGACATA+AGG | 0.503791 | 4.4:-32235237 | MS.gene006598:CDS |
| AGATCACGTGAATATTGCTT+AGG | 0.512635 | 4.4:-32235373 | MS.gene006598:CDS |
| TTGGAACTGATCATAATATG+TGG | 0.512688 | 4.4:-32234945 | MS.gene006598:CDS |
| AGGCTGTATTTCCACATCAC+TGG | 0.522661 | 4.4:+32234987 | None:intergenic |
| ATACAACCAAAGTTGAAAGA+AGG | 0.526780 | 4.4:-32235893 | MS.gene006598:CDS |
| TATAATTCAGGAATTACCGT+TGG | 0.530375 | 4.4:-32235503 | MS.gene006598:intron |
| ACTTGATCCGGTAGCGCCAA+CGG | 0.534008 | 4.4:+32235487 | None:intergenic |
| ATTTCTTGAATTTCAAAACA+TGG | 0.541430 | 4.4:+32235337 | None:intergenic |
| CCTTCATGTTGTTCATGCTG+AGG | 0.541669 | 4.4:-32235179 | MS.gene006598:intron |
| AACCAAAGTTGAAAGAAGGC+TGG | 0.543583 | 4.4:-32235889 | MS.gene006598:CDS |
| TGGAACTGATCATAATATGT+GGG | 0.553773 | 4.4:-32234944 | MS.gene006598:CDS |
| CTACAAATCATTCTCACGTG+TGG | 0.558837 | 4.4:-32235304 | MS.gene006598:CDS |
| AGTGTCAATTCCTCTAAGGT+TGG | 0.561526 | 4.4:-32235214 | MS.gene006598:CDS |
| ATTTCAAAATCCCACAACTC+AGG | 0.570407 | 4.4:+32234967 | None:intergenic |
| AAGTGAGTGAGAGATTGAGA+AGG | 0.570785 | 4.4:-32235026 | MS.gene006598:CDS |
| TGGCTAATGAGAAGAAAGAA+AGG | 0.571122 | 4.4:-32235417 | MS.gene006598:CDS |
| TACAAATCATTCTCACGTGT+GGG | 0.575180 | 4.4:-32235303 | MS.gene006598:CDS |
| GCTCCAAATTGAACACAAGA+TGG | 0.580457 | 4.4:+32235054 | None:intergenic |
| GGAAATACAGCCTGAGTTGT+GGG | 0.582012 | 4.4:-32234977 | MS.gene006598:CDS |
| TTTAGAGGCAAATGTGCAGA+TGG | 0.585387 | 4.4:-32235437 | MS.gene006598:CDS |
| TGGAAATACAGCCTGAGTTG+TGG | 0.593486 | 4.4:-32234978 | MS.gene006598:CDS |
| CCTCAGCATGAACAACATGA+AGG | 0.636646 | 4.4:+32235179 | None:intergenic |
| GGAACTGATCATAATATGTG+GGG | 0.640483 | 4.4:-32234943 | MS.gene006598:CDS |
| GTATGGATCTTCCAGTGATG+TGG | 0.643608 | 4.4:-32234998 | MS.gene006598:CDS |
| TCTCCCTAACTATAACCCCA+AGG | 0.657270 | 4.4:-32235811 | MS.gene006598:intron |
| CCAAATTGAACACAAGATGG+AGG | 0.671279 | 4.4:+32235057 | None:intergenic |
| GCTACCGGATCAAGTAGATG+AGG | 0.685176 | 4.4:-32235479 | MS.gene006598:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAAAAAAAAAAAAAGT+TGG | - | chr4.4:32235118-32235137 | MS.gene006598:intron | 10.0% |
| !! | AAACTACATAAAAAGTTTAG+AGG | - | chr4.4:32235414-32235433 | MS.gene006598:CDS | 20.0% |
| !! | ATTTCTTGAATTTCAAAACA+TGG | + | chr4.4:32235532-32235551 | None:intergenic | 20.0% |
| !! | TGATTTCATAGAAAATGAAT+TGG | + | chr4.4:32235597-32235616 | None:intergenic | 20.0% |
| !!! | TGATGTCATTTTTATAATTC+AGG | - | chr4.4:32235351-32235370 | MS.gene006598:CDS | 20.0% |
| !!! | TGTTTTGAAATTCAAGAAAT+TGG | - | chr4.4:32235532-32235551 | MS.gene006598:intron | 20.0% |
| ! | AAAGAAAGAAATTAGAACCT+TGG | + | chr4.4:32235075-32235094 | None:intergenic | 25.0% |
| ! | AAGAAAACAACTAACTAACA+TGG | + | chr4.4:32235327-32235346 | None:intergenic | 25.0% |
| ! | AAGAAAGAAATTAGAACCTT+GGG | + | chr4.4:32235074-32235093 | None:intergenic | 25.0% |
| ! | ATAAAGAGAGAACAATTACT+AGG | + | chr4.4:32235782-32235801 | None:intergenic | 25.0% |
| !! | AAAAAAAAAGTTGGTTACAC+TGG | - | chr4.4:32235127-32235146 | MS.gene006598:intron | 25.0% |
| !! | ATTTCAAAACATGGTGATTT+TGG | + | chr4.4:32235523-32235542 | None:intergenic | 25.0% |
| !!! | ACTAACATGGTTTTAATTTG+TGG | + | chr4.4:32235314-32235333 | None:intergenic | 25.0% |
| AAGAAAGAAAGGTTATTAGC+TGG | - | chr4.4:32235460-32235479 | MS.gene006598:CDS | 30.0% | |
| AGAAAGAAATTAGAACCTTG+GGG | + | chr4.4:32235073-32235092 | None:intergenic | 30.0% | |
| ATACAACCAAAGTTGAAAGA+AGG | - | chr4.4:32234973-32234992 | MS.gene006598:CDS | 30.0% | |
| ATGAAGAGAATGTAGACATA+AGG | - | chr4.4:32235629-32235648 | MS.gene006598:intron | 30.0% | |
| TATAATTCAGGAATTACCGT+TGG | - | chr4.4:32235363-32235382 | MS.gene006598:CDS | 30.0% | |
| TGGAACTGATCATAATATGT+GGG | - | chr4.4:32235922-32235941 | MS.gene006598:CDS | 30.0% | |
| TTGGAACTGATCATAATATG+TGG | - | chr4.4:32235921-32235940 | MS.gene006598:CDS | 30.0% | |
| AGATCACGTGAATATTGCTT+AGG | - | chr4.4:32235493-32235512 | MS.gene006598:CDS | 35.0% | |
| AGATTGAGAAGGTTTGTGTA+TGG | - | chr4.4:32235851-32235870 | MS.gene006598:CDS | 35.0% | |
| ATTTCAAAATCCCACAACTC+AGG | + | chr4.4:32235902-32235921 | None:intergenic | 35.0% | |
| CCACAATATTGTGGATGTTA+CGG | + | chr4.4:32235267-32235286 | None:intergenic | 35.0% | |
| GGAACTGATCATAATATGTG+GGG | - | chr4.4:32235923-32235942 | MS.gene006598:CDS | 35.0% | |
| TACAAATCATTCTCACGTGT+GGG | - | chr4.4:32235563-32235582 | MS.gene006598:intron | 35.0% | |
| TGAATTATCTCCAACCTTAG+AGG | + | chr4.4:32235665-32235684 | None:intergenic | 35.0% | |
| TGGCTAATGAGAAGAAAGAA+AGG | - | chr4.4:32235449-32235468 | MS.gene006598:CDS | 35.0% | |
| TTGGTTGTATTAGAGCTAGA+AGG | + | chr4.4:32234963-32234982 | None:intergenic | 35.0% | |
| !!! | GAGTTGTGGGATTTTGAAAT+TGG | - | chr4.4:32235902-32235921 | MS.gene006598:CDS | 35.0% |
| AACCAAAGTTGAAAGAAGGC+TGG | - | chr4.4:32234977-32234996 | MS.gene006598:CDS | 40.0% | |
| AACCAGCCTTCTTTCAACTT+TGG | + | chr4.4:32234982-32235001 | None:intergenic | 40.0% | |
| AAGCCACAATATTGTGGTTG+TGG | - | chr4.4:32235225-32235244 | MS.gene006598:CDS | 40.0% | |
| AAGTGAGTGAGAGATTGAGA+AGG | - | chr4.4:32235840-32235859 | MS.gene006598:CDS | 40.0% | |
| AGAACCTTGGGGTTATAGTT+AGG | + | chr4.4:32235062-32235081 | None:intergenic | 40.0% | |
| ATGACGAAGCCACAATATTG+TGG | - | chr4.4:32235219-32235238 | MS.gene006598:CDS | 40.0% | |
| CATCCACAATATTGTGGTTG+TGG | - | chr4.4:32235270-32235289 | MS.gene006598:CDS | 40.0% | |
| CCAAATTGAACACAAGATGG+AGG | + | chr4.4:32235812-32235831 | None:intergenic | 40.0% | |
| CCGTAACATCCACAATATTG+TGG | - | chr4.4:32235264-32235283 | MS.gene006598:CDS | 40.0% | |
| CCTCCATCTTGTGTTCAATT+TGG | - | chr4.4:32235809-32235828 | MS.gene006598:intron | 40.0% | |
| CTACAAATCATTCTCACGTG+TGG | - | chr4.4:32235562-32235581 | MS.gene006598:intron | 40.0% | |
| CTACCACAACCACAATATTG+TGG | + | chr4.4:32235231-32235250 | None:intergenic | 40.0% | |
| GAACCTTGGGGTTATAGTTA+GGG | + | chr4.4:32235061-32235080 | None:intergenic | 40.0% | |
| GCTCCAAATTGAACACAAGA+TGG | + | chr4.4:32235815-32235834 | None:intergenic | 40.0% | |
| TTTAGAGGCAAATGTGCAGA+TGG | - | chr4.4:32235429-32235448 | MS.gene006598:CDS | 40.0% | |
| !! | AAGGAGTGTCAATTCCTCTA+AGG | - | chr4.4:32235648-32235667 | MS.gene006598:intron | 40.0% |
| !! | AGTGTCAATTCCTCTAAGGT+TGG | - | chr4.4:32235652-32235671 | MS.gene006598:intron | 40.0% |
| AGGCTGTATTTCCACATCAC+TGG | + | chr4.4:32235882-32235901 | None:intergenic | 45.0% | |
| AGGGAGAAGAGAGTTGAGTT+TGG | + | chr4.4:32235042-32235061 | None:intergenic | 45.0% | |
| ATCGCCTCATCTACTTGATC+CGG | + | chr4.4:32235394-32235413 | None:intergenic | 45.0% | |
| CCTCAGCATGAACAACATGA+AGG | + | chr4.4:32235690-32235709 | None:intergenic | 45.0% | |
| CCTTCATGTTGTTCATGCTG+AGG | - | chr4.4:32235687-32235706 | MS.gene006598:intron | 45.0% | |
| GGAAATACAGCCTGAGTTGT+GGG | - | chr4.4:32235889-32235908 | MS.gene006598:CDS | 45.0% | |
| GTATGGATCTTCCAGTGATG+TGG | - | chr4.4:32235868-32235887 | MS.gene006598:CDS | 45.0% | |
| TCTCCCTAACTATAACCCCA+AGG | - | chr4.4:32235055-32235074 | MS.gene006598:CDS | 45.0% | |
| TGGAAATACAGCCTGAGTTG+TGG | - | chr4.4:32235888-32235907 | MS.gene006598:CDS | 45.0% | |
| ! | GAGAGTTGAGTTTGGAGTAG+AGG | + | chr4.4:32235034-32235053 | None:intergenic | 45.0% |
| GCTACCGGATCAAGTAGATG+AGG | - | chr4.4:32235387-32235406 | MS.gene006598:CDS | 50.0% | |
| ACTTGATCCGGTAGCGCCAA+CGG | + | chr4.4:32235382-32235401 | None:intergenic | 55.0% | |
| GGAATTACCGTTGGCGCTAC+CGG | - | chr4.4:32235372-32235391 | MS.gene006598:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 32234936 | 32235952 | 32234936 | ID=MS.gene006598 |
| chr4.4 | mRNA | 32234936 | 32235952 | 32234936 | ID=MS.gene006598.t1;Parent=MS.gene006598 |
| chr4.4 | exon | 32235812 | 32235952 | 32235812 | ID=MS.gene006598.t1.exon1;Parent=MS.gene006598.t1 |
| chr4.4 | CDS | 32235812 | 32235952 | 32235812 | ID=cds.MS.gene006598.t1;Parent=MS.gene006598.t1 |
| chr4.4 | exon | 32235180 | 32235515 | 32235180 | ID=MS.gene006598.t1.exon2;Parent=MS.gene006598.t1 |
| chr4.4 | CDS | 32235180 | 32235515 | 32235180 | ID=cds.MS.gene006598.t1;Parent=MS.gene006598.t1 |
| chr4.4 | exon | 32234936 | 32235082 | 32234936 | ID=MS.gene006598.t1.exon3;Parent=MS.gene006598.t1 |
| chr4.4 | CDS | 32234936 | 32235082 | 32234936 | ID=cds.MS.gene006598.t1;Parent=MS.gene006598.t1 |
| Gene Sequence |
| Protein sequence |
