AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006628

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006628.t1 MTR_4g078810 92.272 427 27 4 1 424 1 424 0.0 711
MS.gene006628.t1 MTR_2g090675 61.937 444 124 19 1 421 1 422 7.71e-156 447
MS.gene006628.t1 MTR_4g098870 35.789 285 140 9 145 421 299 548 1.53e-43 160
MS.gene006628.t1 MTR_4g098870 35.789 285 140 9 145 421 319 568 1.74e-43 160
MS.gene006628.t1 MTR_4g098870 35.789 285 140 9 145 421 338 587 2.52e-43 160
MS.gene006628.t1 MTR_5g014040 34.304 309 145 10 121 415 267 531 3.24e-42 156
MS.gene006628.t1 MTR_5g014040 33.758 314 145 11 121 415 227 496 3.69e-40 150
MS.gene006628.t1 MTR_5g014040 33.758 314 145 11 121 415 267 536 4.75e-40 150
MS.gene006628.t1 MTR_4g098870 40.288 139 70 3 145 275 299 432 2.62e-26 111
MS.gene006628.t1 MTR_4g098870 40.288 139 70 3 145 275 319 452 3.27e-26 111
MS.gene006628.t1 MTR_1g032570 31.818 198 114 6 81 277 68 245 1.22e-18 88.6
MS.gene006628.t1 MTR_1g032570 30.808 198 116 5 81 277 137 314 1.41e-18 88.6
MS.gene006628.t1 MTR_4g021760 39.785 93 55 1 120 212 134 225 3.02e-18 85.5
MS.gene006628.t1 MTR_3g106220 48.684 76 39 0 148 223 190 265 8.03e-18 86.3
MS.gene006628.t1 MTR_1g013170 56.061 66 29 0 153 218 188 253 1.04e-16 82.4
MS.gene006628.t1 MTR_7g117705 37.500 120 60 3 151 255 186 305 1.13e-16 82.0
MS.gene006628.t1 MTR_2g450070 54.839 62 28 0 151 212 208 269 4.30e-16 80.9
MS.gene006628.t1 MTR_2g450070 54.839 62 28 0 151 212 208 269 4.36e-16 80.9
MS.gene006628.t1 MTR_4g121020 56.667 60 26 0 155 214 110 169 6.53e-16 80.1
MS.gene006628.t1 MTR_4g121020 56.667 60 26 0 155 214 214 273 8.05e-16 80.1
MS.gene006628.t1 MTR_4g121020 56.667 60 26 0 155 214 214 273 8.13e-16 80.1
MS.gene006628.t1 MTR_7g026400 57.812 64 27 0 145 208 146 209 1.15e-15 79.3
MS.gene006628.t1 MTR_1g013180 56.667 60 26 0 153 212 339 398 2.33e-15 78.6
MS.gene006628.t1 MTR_4g021790 39.785 93 55 1 120 212 133 224 3.25e-15 75.9
MS.gene006628.t1 MTR_2g034960 40.230 87 47 1 155 236 210 296 1.06e-14 76.6
MS.gene006628.t1 MTR_7g089010 44.872 78 37 1 137 214 78 149 1.17e-14 74.7
MS.gene006628.t1 MTR_4g021855 41.573 89 51 1 124 212 137 224 4.27e-14 73.2
MS.gene006628.t1 MTR_8g079940 47.826 69 36 0 155 223 208 276 5.36e-14 74.3
MS.gene006628.t1 MTR_4g086835 42.045 88 47 1 152 239 270 353 5.83e-14 73.6
MS.gene006628.t1 MTR_4g064730 39.506 81 49 0 143 223 118 198 5.90e-14 72.8
MS.gene006628.t1 MTR_3g102600 50.000 54 27 0 161 214 88 141 9.74e-14 69.7
MS.gene006628.t1 MTR_4g113140 36.000 125 60 4 108 212 168 292 1.45e-13 72.4
MS.gene006628.t1 MTR_3g102600 50.000 54 27 0 161 214 137 190 2.05e-13 69.7
MS.gene006628.t1 MTR_4g021845 37.805 82 50 1 124 205 137 217 2.84e-13 69.3
MS.gene006628.t1 MTR_3g086100 43.662 71 40 0 153 223 125 195 3.45e-13 71.6
MS.gene006628.t1 MTR_3g102590 51.786 56 27 0 153 208 140 195 3.83e-13 69.3
MS.gene006628.t1 MTR_3g086100 43.662 71 40 0 153 223 196 266 5.51e-13 71.2
MS.gene006628.t1 MTR_4g131600 41.176 85 48 1 153 235 198 282 7.79e-13 70.5
MS.gene006628.t1 MTR_4g086835 41.573 89 47 2 152 239 270 354 1.77e-12 68.9
MS.gene006628.t1 MTR_4g131570 48.214 56 29 0 153 208 200 255 3.36e-12 68.6
MS.gene006628.t1 MTR_3g102600 48.936 47 24 0 161 207 88 134 3.88e-12 63.9
MS.gene006628.t1 MTR_5g041350 45.333 75 35 3 158 231 17 86 7.13e-12 67.0
MS.gene006628.t1 MTR_1g093080 36.275 102 52 2 147 235 202 303 8.46e-12 66.6
MS.gene006628.t1 MTR_1g053830 49.057 53 26 1 157 208 19 71 9.40e-12 61.6
MS.gene006628.t1 MTR_0450s0040 44.118 68 38 0 155 222 168 235 1.24e-11 65.9
MS.gene006628.t1 MTR_4g131580 48.214 56 29 0 153 208 197 252 1.87e-11 66.2
MS.gene006628.t1 MTR_3g102600 48.936 47 24 0 161 207 137 183 2.48e-11 62.8
MS.gene006628.t1 MTR_6g032990 54.545 55 24 1 158 211 44 98 2.69e-11 65.5
MS.gene006628.t1 MTR_1g080330 46.377 69 34 2 144 211 165 231 3.94e-11 63.9
MS.gene006628.t1 MTR_1g080330 46.377 69 34 2 144 211 165 231 5.37e-11 64.3
MS.gene006628.t1 MTR_5g017980 51.786 56 27 0 155 210 192 247 5.57e-11 63.9
MS.gene006628.t1 MTR_5g017980 51.786 56 27 0 155 210 191 246 5.90e-11 63.9
MS.gene006628.t1 MTR_3g102590 41.270 63 37 0 143 205 130 192 8.84e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006628.t1 AT2G20570 45.495 455 180 21 1 423 1 419 2.13e-81 257
MS.gene006628.t1 AT2G20570 43.949 471 180 22 1 423 1 435 2.26e-77 247
MS.gene006628.t1 AT5G44190 41.348 445 175 16 1 420 1 384 6.51e-72 231
MS.gene006628.t1 AT5G44190 43.345 293 122 10 1 277 1 265 1.14e-59 198
MS.gene006628.t1 AT2G20570 36.424 453 177 21 1 423 1 372 6.81e-40 147
MS.gene006628.t1 AT4G18020 32.266 406 186 12 37 423 191 526 9.56e-36 138
MS.gene006628.t1 AT4G18020 32.266 406 186 12 37 423 191 526 9.56e-36 138
MS.gene006628.t1 AT4G18020 32.266 406 186 12 37 423 191 526 9.56e-36 138
MS.gene006628.t1 AT4G18020 32.266 406 186 12 37 423 191 526 9.56e-36 138
MS.gene006628.t1 AT4G18020 32.266 406 186 12 37 423 191 526 9.56e-36 138
MS.gene006628.t1 AT4G18020 34.483 232 134 4 37 264 191 408 4.67e-30 122
MS.gene006628.t1 AT4G18020 34.483 232 134 4 37 264 191 408 4.67e-30 122
MS.gene006628.t1 AT4G18020 34.483 232 134 4 37 264 191 408 4.67e-30 122
MS.gene006628.t1 AT4G18020 34.483 232 134 4 37 264 191 408 4.67e-30 122
MS.gene006628.t1 AT4G18020 34.483 232 134 4 37 264 191 408 4.67e-30 122
MS.gene006628.t1 AT4G18020 34.483 232 134 4 37 264 191 408 4.67e-30 122
MS.gene006628.t1 AT5G58080 54.839 62 28 0 153 214 191 252 2.84e-16 81.6
MS.gene006628.t1 AT3G10760 41.772 79 46 0 136 214 85 163 2.43e-15 77.0
MS.gene006628.t1 AT2G01760 53.125 64 30 0 151 214 130 193 3.22e-15 76.6
MS.gene006628.t1 AT3G16857 39.815 108 56 2 107 214 127 225 3.98e-15 77.8
MS.gene006628.t1 AT2G25180 51.613 62 30 0 153 214 192 253 4.41e-15 77.8
MS.gene006628.t1 AT3G16857 47.500 80 41 1 135 214 217 295 4.86e-15 77.8
MS.gene006628.t1 AT3G16857 39.815 108 56 2 107 214 197 295 6.11e-15 77.4
MS.gene006628.t1 AT5G05090 51.667 60 29 0 155 214 80 139 6.32e-15 75.1
MS.gene006628.t1 AT2G01760 53.125 64 30 0 151 214 195 258 6.67e-15 76.3
MS.gene006628.t1 AT4G31920 54.237 59 27 0 150 208 177 235 6.68e-15 77.0
MS.gene006628.t1 AT5G59570 38.750 80 49 0 152 231 137 216 8.16e-15 75.1
MS.gene006628.t1 AT5G59570 38.750 80 49 0 152 231 137 216 8.16e-15 75.1
MS.gene006628.t1 AT2G01760 53.125 64 30 0 151 214 223 286 8.18e-15 76.3
MS.gene006628.t1 AT3G46640 46.479 71 36 1 155 223 143 213 2.25e-14 74.3
MS.gene006628.t1 AT3G46640 46.479 71 36 1 155 223 143 213 2.25e-14 74.3
MS.gene006628.t1 AT3G46640 46.479 71 36 1 155 223 143 213 4.50e-14 73.2
MS.gene006628.t1 AT2G40970 43.210 81 43 1 137 214 83 163 7.48e-14 71.6
MS.gene006628.t1 AT5G07210 47.945 73 34 1 152 224 218 286 8.94e-14 73.6
MS.gene006628.t1 AT5G07210 47.945 73 34 1 152 224 226 294 9.18e-14 73.6
MS.gene006628.t1 AT2G03500 39.423 104 62 1 148 250 225 328 1.12e-13 72.8
MS.gene006628.t1 AT1G13300 42.222 90 48 3 124 212 152 238 2.17e-12 68.6
MS.gene006628.t1 AT1G68670 37.037 108 63 2 134 236 193 300 5.04e-12 67.4
MS.gene006628.t1 AT2G27070 36.207 116 71 1 100 215 172 284 8.50e-12 67.4
MS.gene006628.t1 AT1G67710 33.884 121 70 2 104 214 67 187 1.73e-11 66.2
MS.gene006628.t1 AT1G67710 33.884 121 70 2 104 214 58 178 2.02e-11 65.9
MS.gene006628.t1 AT3G04030 55.769 52 22 1 158 208 47 98 2.04e-11 65.9
MS.gene006628.t1 AT3G04030 55.769 52 22 1 158 208 47 98 2.39e-11 65.5
MS.gene006628.t1 AT5G18240 57.407 54 22 1 156 208 45 98 3.21e-11 65.1
MS.gene006628.t1 AT5G18240 57.407 54 22 1 156 208 45 98 3.21e-11 65.1
MS.gene006628.t1 AT1G67710 33.884 121 70 2 104 214 132 252 3.26e-11 65.5
MS.gene006628.t1 AT1G25550 41.892 74 42 1 141 213 194 267 3.31e-11 64.7
MS.gene006628.t1 AT5G18240 57.407 54 22 1 156 208 45 98 3.66e-11 65.1
MS.gene006628.t1 AT5G18240 57.407 54 22 1 156 208 45 98 3.82e-11 65.1
MS.gene006628.t1 AT5G18240 57.407 54 22 1 156 208 45 98 3.82e-11 65.1
MS.gene006628.t1 AT4G28610 31.933 119 70 2 94 211 173 281 6.11e-11 64.3

Find 128 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGTGCCTTCAAGGATTT+TGG 0.156203 4.4:-31910869 MS.gene006628:CDS
CACCTCAAGATCCTTCATTT+TGG 0.206831 4.4:-31910297 MS.gene006628:CDS
CCTTTCCGAAGGAAATTTAT+TGG 0.220430 4.4:-31911963 MS.gene006628:CDS
ATGCCATGGGGTGGGATGCC+GGG 0.306670 4.4:+31908330 None:intergenic
TGGAGACATCTTACCGGATT+TGG 0.308487 4.4:-31911897 MS.gene006628:CDS
GGCCGGATCATTGCGTTTAT+CGG 0.317082 4.4:+31908671 None:intergenic
AAACCCATGGTGGGTGCAGC+TGG 0.319978 4.4:+31910472 None:intergenic
ATGGGGACATCCTTCAAATA+TGG 0.339407 4.4:-31910389 MS.gene006628:CDS
CATGCCATGGGGTGGGATGC+CGG 0.361244 4.4:+31908329 None:intergenic
ATACATGTAAGGGTCTAAAA+TGG 0.371409 4.4:+31910410 None:intergenic
TGGGGTGGGATGCCGGGGAT+TGG 0.376158 4.4:+31908336 None:intergenic
CCAGCACTTGATAGCGTAAT+GGG 0.402444 4.4:-31907877 MS.gene006628:CDS
GAGGATAAAACAAGCTGTTC+GGG 0.406204 4.4:-31911752 MS.gene006628:CDS
GAGGAGGAGGAGGATGAGGG+GGG 0.410471 4.4:+31910449 None:intergenic
TTAAAAGATGCAGGTGGAAA+AGG 0.411700 4.4:-31911671 MS.gene006628:CDS
GATGAATTCTTCGGCGTCAC+TGG 0.421305 4.4:-31911819 MS.gene006628:CDS
TGAGGATAAAACAAGCTGTT+CGG 0.423559 4.4:-31911753 MS.gene006628:CDS
GAGAGCATAGACGCAGCTAT+TGG 0.425120 4.4:-31907949 MS.gene006628:CDS
ATAATCTCCAAAATCCTTGA+AGG 0.430397 4.4:+31910862 None:intergenic
CTCCGATAAACGCAATGATC+CGG 0.431873 4.4:-31908673 MS.gene006628:CDS
CATCTTACCGGATTTGGTAA+TGG 0.436295 4.4:-31911891 MS.gene006628:CDS
TAACATTGCTAGTCACCTTC+AGG 0.436461 4.4:-31910815 MS.gene006628:intron
GAGAACTCAGCAAACATCTC+TGG 0.443524 4.4:+31911866 None:intergenic
ACCACTGTTGTCAGAGGTGG+TGG 0.450953 4.4:+31908632 None:intergenic
TGGACAAGGCGGTGCCTTCA+AGG 0.452098 4.4:-31910876 MS.gene006628:CDS
TCTGGATCCATTACCAAATC+CGG 0.453183 4.4:+31911884 None:intergenic
ACAATTACAACGGGCGACTT+CGG 0.456230 4.4:-31911989 MS.gene006628:CDS
GGTGGGAAACAATGTGTTCC+TGG 0.458402 4.4:+31908650 None:intergenic
ATGGTGGGTGCAGCTGGCCA+TGG 0.458606 4.4:+31910478 None:intergenic
GCTTTCCAATAAATTTCCTT+CGG 0.459139 4.4:+31911958 None:intergenic
TCGAAGAAGTGACCGGGAGC+AGG 0.463586 4.4:+31908276 None:intergenic
ACAAATCTTCTGTGCAAGTC+TGG 0.463854 4.4:+31910922 None:intergenic
CATGAGAGTGGCTGCGGTGT+AGG 0.464099 4.4:+31910343 None:intergenic
AATCAAAGGAGACAAGTATA+TGG 0.464153 4.4:-31910532 MS.gene006628:CDS
GAAGGTGACTAGCAATGTTA+TGG 0.464906 4.4:+31910818 None:intergenic
GAATTCAAAGAAAGATTATG+AGG 0.466750 4.4:-31911771 MS.gene006628:CDS
TGCCATGGGGTGGGATGCCG+GGG 0.471359 4.4:+31908331 None:intergenic
AAGCAGCAAGTTGGAATCAA+AGG 0.471928 4.4:-31910546 MS.gene006628:CDS
GCTGAGTTCTCCGTTAGTTA+CGG 0.472120 4.4:-31911854 MS.gene006628:CDS
ATGGCCAGCTGCACCCACCA+TGG 0.477260 4.4:-31910476 MS.gene006628:CDS
TGTTTATTGTGCAGGTGGAT+TGG 0.477271 4.4:-31910948 MS.gene006628:intron
AGACAAGTATATGGTGGTGT+AGG 0.478471 4.4:-31910523 MS.gene006628:CDS
GACAAGTATATGGTGGTGTA+GGG 0.478876 4.4:-31910522 MS.gene006628:CDS
TGGCCAGCTGCACCCACCAT+GGG 0.485571 4.4:-31910475 MS.gene006628:CDS
CCATGGTTGCCACTACCACT+TGG 0.489750 4.4:-31907910 MS.gene006628:CDS
AACATGCAGAGACCATCATT+TGG 0.489976 4.4:-31908363 MS.gene006628:intron
CGTCGATGGAGACATCTTAC+CGG 0.493741 4.4:-31911903 MS.gene006628:CDS
GATCTGCTTTACACATGCCA+TGG 0.496492 4.4:+31908316 None:intergenic
TCTTTGTTAGTGTTCCTCAA+TGG 0.497869 4.4:+31912055 None:intergenic
TCCAGCACTTGATAGCGTAA+TGG 0.499694 4.4:-31907878 MS.gene006628:CDS
ATTAGCACCCTTCCTGCTCC+CGG 0.500715 4.4:-31908288 MS.gene006628:CDS
GGGAGTACTACAATTACAAC+GGG 0.501212 4.4:-31911998 MS.gene006628:CDS
AGGATGTCCCCATACATGTA+AGG 0.503004 4.4:+31910399 None:intergenic
GAAGTGACCGGGAGCAGGAA+GGG 0.509953 4.4:+31908281 None:intergenic
CCAATAAATTTCCTTCGGAA+AGG 0.511123 4.4:+31911963 None:intergenic
TTGAGGAACACTAACAAAGA+TGG 0.514130 4.4:-31912052 MS.gene006628:CDS
CCCATTACGCTATCAAGTGC+TGG 0.514753 4.4:+31907877 None:intergenic
ATCTGCTTTACACATGCCAT+GGG 0.515933 4.4:+31908317 None:intergenic
TGATTCCTCGCCGTAACTAA+CGG 0.519226 4.4:+31911844 None:intergenic
GAAGGATCTTGAGGTGGTGG+AGG 0.522935 4.4:+31910304 None:intergenic
GGAATCAGAGATGAATTCTT+CGG 0.526908 4.4:-31911828 MS.gene006628:CDS
AATGAAGGATCTTGAGGTGG+TGG 0.528793 4.4:+31910301 None:intergenic
GCTTTGAGTCCAAGTGGTAG+TGG 0.528879 4.4:+31907901 None:intergenic
AGATGGAAACCAAGAAGAGA+TGG 0.529997 4.4:-31912035 MS.gene006628:CDS
TTTGTTAGCATCAACGTCGA+TGG 0.530835 4.4:-31911917 MS.gene006628:CDS
AAATGGTGCAATGGTGCCAT+AGG 0.534447 4.4:+31910427 None:intergenic
AAAGACTGGTCCATATTTGA+AGG 0.536097 4.4:+31910379 None:intergenic
GGAAACAATGTGTTCCTGGC+CGG 0.538523 4.4:+31908654 None:intergenic
TTTCTTCAAGTGCATGATGA+GGG 0.538544 4.4:+31907820 None:intergenic
ATGGTGGTGTAGGGGTGAAG+AGG 0.540082 4.4:-31910513 MS.gene006628:CDS
TGGTGGTGTAGGGGTGAAGA+GGG 0.540938 4.4:-31910512 MS.gene006628:CDS
TGGGAAGTCGAAGAAGTGAC+CGG 0.545060 4.4:+31908269 None:intergenic
TGCAATGGTGCCATAGGAGG+AGG 0.545767 4.4:+31910433 None:intergenic
AAAACAAGCTGTTCGGGTCA+AGG 0.545941 4.4:-31911746 MS.gene006628:CDS
AATCCATTGTTAAAAGATGC+AGG 0.548676 4.4:-31911680 MS.gene006628:CDS
GGGATTGGACATCCAAATGA+TGG 0.551089 4.4:+31908351 None:intergenic
CTACACCGCAGCCACTCTCA+TGG 0.554231 4.4:-31910342 MS.gene006628:CDS
AAGCTGTTCGGGTCAAGGCT+CGG 0.557290 4.4:-31911741 MS.gene006628:CDS
TGGTGCAATGGTGCCATAGG+AGG 0.557595 4.4:+31910430 None:intergenic
GCTTTACACATGCCATGGGG+TGG 0.557961 4.4:+31908321 None:intergenic
GGTGCCATAGGAGGAGGAGG+AGG 0.561800 4.4:+31910439 None:intergenic
TTGGCATTCTCACCACCAGC+AGG 0.563830 4.4:-31910278 MS.gene006628:intron
CTTCAAGTGCATGATGAGGG+TGG 0.573822 4.4:+31907823 None:intergenic
TGCCAAAATGAAGGATCTTG+AGG 0.576157 4.4:+31910295 None:intergenic
TCATTTGGATGTCCAATCCC+CGG 0.576190 4.4:-31908348 MS.gene006628:CDS
CTTTACACATGCCATGGGGT+GGG 0.577896 4.4:+31908322 None:intergenic
ATCCCCGGCATCCCACCCCA+TGG 0.583757 4.4:-31908333 MS.gene006628:CDS
GTGCAAGCTGTGGAACAGCT+AGG 0.587705 4.4:-31910901 MS.gene006628:CDS
GTGAAGCAGAAGCAGCAAGT+TGG 0.590428 4.4:-31910555 MS.gene006628:CDS
ATAAACGCAATGATCCGGCC+AGG 0.590506 4.4:-31908668 MS.gene006628:CDS
GGATGTCCCCATACATGTAA+GGG 0.590827 4.4:+31910400 None:intergenic
CCAAGTGGTAGTGGCAACCA+TGG 0.591902 4.4:+31907910 None:intergenic
AATGGTGCCATAGGAGGAGG+AGG 0.593332 4.4:+31910436 None:intergenic
GGTGTCCATGAGAGTGGCTG+CGG 0.595402 4.4:+31910337 None:intergenic
TTTAGACCCTTACATGTATG+GGG 0.601372 4.4:-31910406 MS.gene006628:CDS
TCACTTCTTCGACTTCCCAC+CGG 0.602135 4.4:-31908265 MS.gene006628:intron
GCCACTCTCATGGACACCAT+CGG 0.612134 4.4:-31910332 MS.gene006628:CDS
AGAAGTGACCGGGAGCAGGA+AGG 0.612201 4.4:+31908280 None:intergenic
GACTTCGGAGACCTTTCCGA+AGG 0.616050 4.4:-31911974 MS.gene006628:CDS
GCCACATGTGCAGAAAAGAC+TGG 0.621691 4.4:+31910365 None:intergenic
AGCTGTGGAACAGCTAGGAG+TGG 0.623334 4.4:-31910896 MS.gene006628:CDS
AGAGGGAAGTGATCAGTCCA+TGG 0.624850 4.4:-31910495 MS.gene006628:CDS
GGCATGTGTAAAGCAGATCA+CGG 0.631742 4.4:-31908312 MS.gene006628:CDS
CAGAAGATTTGTGCAAGCTG+TGG 0.632319 4.4:-31910911 MS.gene006628:CDS
GGGAAGTCGAAGAAGTGACC+GGG 0.633060 4.4:+31908270 None:intergenic
TGCTTGCAGTGTCACCATTG+AGG 0.634204 4.4:-31912069 MS.gene006628:CDS
ACAAGTATATGGTGGTGTAG+GGG 0.636855 4.4:-31910521 MS.gene006628:CDS
TGGTGAGAATGCCAAAATGA+AGG 0.637260 4.4:+31910286 None:intergenic
ACCGATGGTGTCCATGAGAG+TGG 0.639166 4.4:+31910331 None:intergenic
GTTACCACTGTTGTCAGAGG+TGG 0.641294 4.4:+31908629 None:intergenic
CAAAGGAGACAAGTATATGG+TGG 0.641945 4.4:-31910529 MS.gene006628:CDS
TCAAGCGAAGAGAAAAGTCA+AGG 0.644275 4.4:-31911618 MS.gene006628:intron
ATAGTTACCACTGTTGTCAG+AGG 0.658785 4.4:+31908626 None:intergenic
GGAACAGCTAGGAGTGGACA+AGG 0.659903 4.4:-31910890 MS.gene006628:CDS
TAGAGAGAGCTACCTGCTGG+TGG 0.667063 4.4:+31910266 None:intergenic
GGGGAGTACTACAATTACAA+CGG 0.686337 4.4:-31911999 MS.gene006628:CDS
GGTGGTGGAGGAGCTACCGA+TGG 0.688749 4.4:+31910316 None:intergenic
GAGTAAAAGAGATGAATCTG+TGG 0.689938 4.4:-31911708 MS.gene006628:CDS
GCTGGAGCTTTGAGTCCAAG+TGG 0.690127 4.4:+31907895 None:intergenic
AAGGGTCTAAAATGGTGCAA+TGG 0.690674 4.4:+31910418 None:intergenic
GTTCTCCGTTAGTTACGGCG+AGG 0.691247 4.4:-31911849 MS.gene006628:CDS
ATGGGTGAACTACAAAGACA+AGG 0.697859 4.4:-31907859 MS.gene006628:CDS
CCACTGTTGTCAGAGGTGGT+GGG 0.703169 4.4:+31908633 None:intergenic
CAAAATGAAGGATCTTGAGG+TGG 0.718294 4.4:+31910298 None:intergenic
CCCACCACCTCTGACAACAG+TGG 0.721504 4.4:-31908633 MS.gene006628:intron
CCATTGTTAAAAGATGCAGG+TGG 0.735818 4.4:-31911677 MS.gene006628:CDS
TCTGCTTTACACATGCCATG+GGG 0.759671 4.4:+31908318 None:intergenic
ACAGCTAGGAGTGGACAAGG+CGG 0.769326 4.4:-31910887 MS.gene006628:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTATATATGAGAT+CGG - chr4.4:31909867-31909886 MS.gene006628:intron 10.0%
!! AACCTTAAATAAATATTATT+TGG + chr4.4:31910301-31910320 None:intergenic 10.0%
!! ATAAAAACAAATTATTGTTT+AGG - chr4.4:31909812-31909831 MS.gene006628:intron 10.0%
!! ATAATAACTAAATATCATTT+GGG + chr4.4:31910634-31910653 None:intergenic 10.0%
!! TATAATAACTAAATATCATT+TGG + chr4.4:31910635-31910654 None:intergenic 10.0%
!! TTTGTCATATATTATATATA+AGG - chr4.4:31910797-31910816 MS.gene006628:intron 10.0%
!!! ATGTAAAACTTTTTAATATA+TGG + chr4.4:31911734-31911753 None:intergenic 10.0%
!!! CAAAAAAAAAAAATTTTTTG+AGG + chr4.4:31910946-31910965 None:intergenic 10.0%
!!! TCTCTTTTAATATTTTTTTT+GGG + chr4.4:31909982-31910001 None:intergenic 10.0%
!!! TTCTCTTTTAATATTTTTTT+TGG + chr4.4:31909983-31910002 None:intergenic 10.0%
!!! TTTTATAGCATTTTTTTTTT+CGG - chr4.4:31908615-31908634 MS.gene006628:intron 10.0%
!! CAAATGGTATATATAAAAAT+TGG + chr4.4:31910361-31910380 None:intergenic 15.0%
!! GTCCAAATAATATTTATTTA+AGG - chr4.4:31910296-31910315 MS.gene006628:CDS 15.0%
!! TACTGCTATTATTATTAAAT+AGG - chr4.4:31908737-31908756 MS.gene006628:intron 15.0%
!! TATACAATGATAGATATATA+GGG + chr4.4:31909679-31909698 None:intergenic 15.0%
!! TTACTTATATATAAATTGTG+AGG + chr4.4:31908717-31908736 None:intergenic 15.0%
!! TTGTTATAAATAAAAATTCG+AGG - chr4.4:31911067-31911086 MS.gene006628:intron 15.0%
!!! TAGGAAAATATACAATTTTT+AGG - chr4.4:31909903-31909922 MS.gene006628:intron 15.0%
!!! TTGTTTTAAAATCTTTTTCT+AGG + chr4.4:31910861-31910880 None:intergenic 15.0%
!! AGTACAAACTGAAATAATTA+AGG + chr4.4:31909776-31909795 None:intergenic 20.0%
!! ATATAAATTGTGAGGATATT+TGG + chr4.4:31908709-31908728 None:intergenic 20.0%
!! CTATACAATGATAGATATAT+AGG + chr4.4:31909680-31909699 None:intergenic 20.0%
!! GTACAAACTGAAATAATTAA+GGG + chr4.4:31909775-31909794 None:intergenic 20.0%
!! TATTAAAAAAATGTTAGCAG+TGG - chr4.4:31908578-31908597 MS.gene006628:intron 20.0%
!! TGTGCTATATTTATATATGT+TGG + chr4.4:31911408-31911427 None:intergenic 20.0%
!! TTATTCTCCAAATATAAGTT+TGG - chr4.4:31911478-31911497 MS.gene006628:intron 20.0%
!!! AAAACTTTTTAATATATGGC+CGG + chr4.4:31911730-31911749 None:intergenic 20.0%
!!! AATTCTCTTTATTTAGTTTC+CGG - chr4.4:31911708-31911727 MS.gene006628:CDS 20.0%
!!! ACGATTAAGATTTTAAACTT+TGG - chr4.4:31909739-31909758 MS.gene006628:intron 20.0%
!!! ATTAAGTCACATCAATTTTT+TGG + chr4.4:31910132-31910151 None:intergenic 20.0%
!!! ATTACATCACTTTACTATTT+TGG - chr4.4:31911294-31911313 MS.gene006628:intron 20.0%
!!! CTTTTAGGTGTTTTTATATT+GGG + chr4.4:31911841-31911860 None:intergenic 20.0%
!!! GAAAATATACAATTTTTAGG+AGG - chr4.4:31909906-31909925 MS.gene006628:intron 20.0%
!!! TCTTATGTTATTTTCCTTTA+TGG + chr4.4:31911508-31911527 None:intergenic 20.0%
!!! TGTCAATCTTATCTTTAAAA+AGG + chr4.4:31911356-31911375 None:intergenic 20.0%
!!! TTTGTCGACTTTTATATTTT+AGG - chr4.4:31910469-31910488 MS.gene006628:CDS 20.0%
!!! TTTTAGGTGTTTTTATATTG+GGG + chr4.4:31911840-31911859 None:intergenic 20.0%
!!! TTTTGAATATAACGAACAAA+TGG + chr4.4:31910377-31910396 None:intergenic 20.0%
!!! TTTTGTTATTGCAGAAATAT+AGG - chr4.4:31909292-31909311 MS.gene006628:intron 20.0%
! AACAAGGATTCAATTATATG+TGG + chr4.4:31910922-31910941 None:intergenic 25.0%
! AATTTGCATATTACATGTCA+TGG - chr4.4:31908826-31908845 MS.gene006628:intron 25.0%
! AGTCAAGGTATACTTAAATA+AGG - chr4.4:31908292-31908311 MS.gene006628:CDS 25.0%
! ATGTTGTTAATTTCAAATCC+CGG - chr4.4:31910164-31910183 MS.gene006628:intron 25.0%
! CTAAGTGGTTTATCTTAATT+TGG - chr4.4:31908337-31908356 MS.gene006628:CDS 25.0%
! GAATTCAAAGAAAGATTATG+AGG - chr4.4:31908124-31908143 MS.gene006628:intron 25.0%
! TGATAGATATATAGGGAATT+TGG + chr4.4:31909672-31909691 None:intergenic 25.0%
! TGTTAACAATTCGAATTTAC+CGG - chr4.4:31908641-31908660 MS.gene006628:CDS 25.0%
! TTCAAGCATATAGAAGAAAA+AGG - chr4.4:31909114-31909133 MS.gene006628:intron 25.0%
!! GTTTTTATATATGAAACCAG+CGG + chr4.4:31909800-31909819 None:intergenic 25.0%
!! TGTGCTCAAATATTGTTATT+TGG + chr4.4:31909952-31909971 None:intergenic 25.0%
!! TTTTTATATATGAAACCAGC+GGG + chr4.4:31909799-31909818 None:intergenic 25.0%
!!! AAAAATTTTTTGAGGGAACA+AGG + chr4.4:31910938-31910957 None:intergenic 25.0%
!!! CACTATTTATCAAGTTTTGA+TGG - chr4.4:31909228-31909247 MS.gene006628:intron 25.0%
!!! GCTTTTAGGTGTTTTTATAT+TGG + chr4.4:31911842-31911861 None:intergenic 25.0%
!!! GTGCTCAAATATTGTTATTT+GGG + chr4.4:31909951-31909970 None:intergenic 25.0%
AAATTAGAGAAAGTCTTTCC+TGG + chr4.4:31910328-31910347 None:intergenic 30.0%
AATCAAAGGAGACAAGTATA+TGG - chr4.4:31909363-31909382 MS.gene006628:intron 30.0%
AATCCATTGTTAAAAGATGC+AGG - chr4.4:31908215-31908234 MS.gene006628:intron 30.0%
AATTAGTGTGTTTATTGTGC+AGG - chr4.4:31908939-31908958 MS.gene006628:intron 30.0%
ACATTATATGAGAGACGATA+AGG - chr4.4:31911750-31911769 MS.gene006628:CDS 30.0%
AGGATTCAATTATATGTGGT+TGG + chr4.4:31910918-31910937 None:intergenic 30.0%
ATAATCTCCAAAATCCTTGA+AGG + chr4.4:31909036-31909055 None:intergenic 30.0%
ATACATGTAAGGGTCTAAAA+TGG + chr4.4:31909488-31909507 None:intergenic 30.0%
CATTATATGAGAGACGATAA+GGG - chr4.4:31911751-31911770 MS.gene006628:CDS 30.0%
CCTAATTACGATGAATATAC+CGG + chr4.4:31908663-31908682 None:intergenic 30.0%
GCTTTCCAATAAATTTCCTT+CGG + chr4.4:31907940-31907959 None:intergenic 30.0%
TAAAAGCTCCATAGATTTCA+AGG - chr4.4:31911855-31911874 MS.gene006628:CDS 30.0%
TGAAATTCATATGTACCTGA+AGG + chr4.4:31909098-31909117 None:intergenic 30.0%
TGAATGCATGTATGTATGTA+TGG + chr4.4:31911672-31911691 None:intergenic 30.0%
TTTATAGTGCGTTGTATGTT+TGG - chr4.4:31908501-31908520 MS.gene006628:intron 30.0%
! ATTTATTTAAGGTTGTGTCC+AGG - chr4.4:31910307-31910326 MS.gene006628:CDS 30.0%
! ATTTTAGACCCTTACATGTA+TGG - chr4.4:31909487-31909506 MS.gene006628:intron 30.0%
! CTGTCTCTATAATTTGTCAA+GGG + chr4.4:31908423-31908442 None:intergenic 30.0%
! GAGATGTTTTATCAAAACCA+TGG - chr4.4:31911968-31911987 MS.gene006628:CDS 30.0%
! TCTGTCTCTATAATTTGTCA+AGG + chr4.4:31908424-31908443 None:intergenic 30.0%
! TTGAAATCTATGGAGCTTTT+AGG + chr4.4:31911856-31911875 None:intergenic 30.0%
! TTGTCTTCTACTCTGAATTT+TGG - chr4.4:31911328-31911347 MS.gene006628:intron 30.0%
! TTTTAGACCCTTACATGTAT+GGG - chr4.4:31909488-31909507 MS.gene006628:intron 30.0%
!! TTCAAGGATTTTGGAGATTA+TGG - chr4.4:31909035-31909054 MS.gene006628:intron 30.0%
!!! AAGTCACATCAATTTTTTGG+TGG + chr4.4:31910129-31910148 None:intergenic 30.0%
!!! AGAGTTTTATTCTTGCTAAG+TGG - chr4.4:31908322-31908341 MS.gene006628:CDS 30.0%
!!! GTGTACTAACTTTTTAGCTA+AGG - chr4.4:31908372-31908391 MS.gene006628:intron 30.0%
AAAAACTCTCCATCTCTTCT+TGG + chr4.4:31907872-31907891 None:intergenic 35.0%
ACAACACATGGTAATATGCA+AGG - chr4.4:31910066-31910085 MS.gene006628:intron 35.0%
ATAAGTTTGGCTGACCATAA+AGG - chr4.4:31911491-31911510 MS.gene006628:intron 35.0%
CCAATAAATTTCCTTCGGAA+AGG + chr4.4:31907935-31907954 None:intergenic 35.0%
CCGGTATATTCATCGTAATT+AGG - chr4.4:31908660-31908679 MS.gene006628:CDS 35.0%
CCTTTCCGAAGGAAATTTAT+TGG - chr4.4:31907932-31907951 MS.gene006628:CDS 35.0%
GAGTAAAAGAGATGAATCTG+TGG - chr4.4:31908187-31908206 MS.gene006628:intron 35.0%
GGAATCAGAGATGAATTCTT+CGG - chr4.4:31908067-31908086 MS.gene006628:intron 35.0%
TACTCATGCCTTGAAATCTA+TGG + chr4.4:31911866-31911885 None:intergenic 35.0%
TCGATATGTACAAGTACGTT+TGG + chr4.4:31910723-31910742 None:intergenic 35.0%
TCTTTGTTAGTGTTCCTCAA+TGG + chr4.4:31907843-31907862 None:intergenic 35.0%
TGAGGATAAAACAAGCTGTT+CGG - chr4.4:31908142-31908161 MS.gene006628:intron 35.0%
TGGTCAGCCAAACTTATATT+TGG + chr4.4:31911488-31911507 None:intergenic 35.0%
TTAAAAGATGCAGGTGGAAA+AGG - chr4.4:31908224-31908243 MS.gene006628:intron 35.0%
TTGAGGAACACTAACAAAGA+TGG - chr4.4:31907843-31907862 MS.gene006628:CDS 35.0%
TTTAGACCCTTACATGTATG+GGG - chr4.4:31909489-31909508 MS.gene006628:intron 35.0%
TTTAGGAGGAACACTTACTT+GGG - chr4.4:31909920-31909939 MS.gene006628:intron 35.0%
! AAAGACTGGTCCATATTTGA+AGG + chr4.4:31909519-31909538 None:intergenic 35.0%
! GATAAGGGCATGTGTTTATA+AGG - chr4.4:31911766-31911785 MS.gene006628:CDS 35.0%
!! TTTTAGGAGGAACACTTACT+TGG - chr4.4:31909919-31909938 MS.gene006628:intron 35.0%
!!! AATTTGGCTGCTATTTTCAG+TGG + chr4.4:31909656-31909675 None:intergenic 35.0%
!!! ATCAATTTTTTGGTGGTGTC+CGG + chr4.4:31910122-31910141 None:intergenic 35.0%
!!! GTTCTTCTTCTTTTTTGACG+AGG + chr4.4:31910972-31910991 None:intergenic 35.0%
AACATGCAGAGACCATCATT+TGG - chr4.4:31911532-31911551 MS.gene006628:intron 40.0%
ACAAATCTTCTGTGCAAGTC+TGG + chr4.4:31908976-31908995 None:intergenic 40.0%
AGATGGAAACCAAGAAGAGA+TGG - chr4.4:31907860-31907879 MS.gene006628:CDS 40.0%
AGGGCATGTGTTTATAAGGT+GGG - chr4.4:31911770-31911789 MS.gene006628:CDS 40.0%
ATAGTTACCACTGTTGTCAG+AGG + chr4.4:31911272-31911291 None:intergenic 40.0%
ATCTGCTTTACACATGCCAT+GGG + chr4.4:31911581-31911600 None:intergenic 40.0%
ATGGGGACATCCTTCAAATA+TGG - chr4.4:31909506-31909525 MS.gene006628:intron 40.0%
ATGGGTGAACTACAAAGACA+AGG - chr4.4:31912036-31912055 MS.gene006628:CDS 40.0%
CAAAATGAAGGATCTTGAGG+TGG + chr4.4:31909600-31909619 None:intergenic 40.0%
CAAAGGAGACAAGTATATGG+TGG - chr4.4:31909366-31909385 MS.gene006628:intron 40.0%
CATGTATGTATGTATGGCGA+AGG + chr4.4:31911666-31911685 None:intergenic 40.0%
CCATTGTTAAAAGATGCAGG+TGG - chr4.4:31908218-31908237 MS.gene006628:intron 40.0%
GAAGGTGACTAGCAATGTTA+TGG + chr4.4:31909080-31909099 None:intergenic 40.0%
GAGGATAAAACAAGCTGTTC+GGG - chr4.4:31908143-31908162 MS.gene006628:intron 40.0%
GCAACTGTTCAAAATTTCGC+TGG - chr4.4:31908461-31908480 MS.gene006628:intron 40.0%
GTACTCGTACGATGTCTTTA+TGG - chr4.4:31909181-31909200 MS.gene006628:intron 40.0%
TAACATTGCTAGTCACCTTC+AGG - chr4.4:31909080-31909099 MS.gene006628:intron 40.0%
TAGGATCTTCATCTCTAACG+AGG - chr4.4:31908679-31908698 MS.gene006628:intron 40.0%
TAGTGTGTTTATTGTGCAGG+TGG - chr4.4:31908942-31908961 MS.gene006628:intron 40.0%
TCAAGCGAAGAGAAAAGTCA+AGG - chr4.4:31908277-31908296 MS.gene006628:CDS 40.0%
TCTGGATCCATTACCAAATC+CGG + chr4.4:31908014-31908033 None:intergenic 40.0%
TGCCAAAATGAAGGATCTTG+AGG + chr4.4:31909603-31909622 None:intergenic 40.0%
TGGTGAGAATGCCAAAATGA+AGG + chr4.4:31909612-31909631 None:intergenic 40.0%
TGTTTATTGTGCAGGTGGAT+TGG - chr4.4:31908947-31908966 MS.gene006628:intron 40.0%
TTTGTTAGCATCAACGTCGA+TGG - chr4.4:31907978-31907997 MS.gene006628:intron 40.0%
! AAGCAGCAAGTTGGAATCAA+AGG - chr4.4:31909349-31909368 MS.gene006628:intron 40.0%
! AAGGGCATGTGTTTATAAGG+TGG - chr4.4:31911769-31911788 MS.gene006628:CDS 40.0%
! AAGGGTCTAAAATGGTGCAA+TGG + chr4.4:31909480-31909499 None:intergenic 40.0%
! ACAAGTATATGGTGGTGTAG+GGG - chr4.4:31909374-31909393 MS.gene006628:intron 40.0%
! AGACAAGTATATGGTGGTGT+AGG - chr4.4:31909372-31909391 MS.gene006628:intron 40.0%
! CACCTCAAGATCCTTCATTT+TGG - chr4.4:31909598-31909617 MS.gene006628:intron 40.0%
! CATCTTACCGGATTTGGTAA+TGG - chr4.4:31908004-31908023 MS.gene006628:intron 40.0%
! CCACCTGCATCTTTTAACAA+TGG + chr4.4:31908221-31908240 None:intergenic 40.0%
! GACAAGTATATGGTGGTGTA+GGG - chr4.4:31909373-31909392 MS.gene006628:intron 40.0%
! TTGACTTTTCTCTTCGCTTG+AGG + chr4.4:31908278-31908297 None:intergenic 40.0%
!! AGAGATGGAGAGTTTTTCGA+TGG - chr4.4:31907875-31907894 MS.gene006628:CDS 40.0%
!! AGATGGAGAGTTTTTCGATG+GGG - chr4.4:31907877-31907896 MS.gene006628:CDS 40.0%
!! AGTGTCTCGTGCACTTTTTA+AGG + chr4.4:31908899-31908918 None:intergenic 40.0%
!! GAGATGGAGAGTTTTTCGAT+GGG - chr4.4:31907876-31907895 MS.gene006628:CDS 40.0%
!! GATCGGAGAGAGTATTTGAT+AGG - chr4.4:31909884-31909903 MS.gene006628:intron 40.0%
!! GGGAGTACTACAATTACAAC+GGG - chr4.4:31907897-31907916 MS.gene006628:CDS 40.0%
!! GGGGAGTACTACAATTACAA+CGG - chr4.4:31907896-31907915 MS.gene006628:CDS 40.0%
!!! TCAATTTTTTGGTGGTGTCC+GGG + chr4.4:31910121-31910140 None:intergenic 40.0%
AAAACAAGCTGTTCGGGTCA+AGG - chr4.4:31908149-31908168 MS.gene006628:intron 45.0%
AATGAAGGATCTTGAGGTGG+TGG + chr4.4:31909597-31909616 None:intergenic 45.0%
ACAATTACAACGGGCGACTT+CGG - chr4.4:31907906-31907925 MS.gene006628:CDS 45.0%
AGAGTAGAAGACAACGTGCA+TGG + chr4.4:31911322-31911341 None:intergenic 45.0%
AGGATGTCCCCATACATGTA+AGG + chr4.4:31909499-31909518 None:intergenic 45.0%
ATGCAAGGTCCAGATATGCA+AGG - chr4.4:31910081-31910100 MS.gene006628:intron 45.0%
ATTTCAGTTTGTACTCCCGC+TGG - chr4.4:31909781-31909800 MS.gene006628:intron 45.0%
CAGAAGATTTGTGCAAGCTG+TGG - chr4.4:31908984-31909003 MS.gene006628:intron 45.0%
CTCCGATAAACGCAATGATC+CGG - chr4.4:31911222-31911241 MS.gene006628:intron 45.0%
GAGAACTCAGCAAACATCTC+TGG + chr4.4:31908032-31908051 None:intergenic 45.0%
GATCTGCTTTACACATGCCA+TGG + chr4.4:31911582-31911601 None:intergenic 45.0%
GCTGAGTTCTCCGTTAGTTA+CGG - chr4.4:31908041-31908060 MS.gene006628:intron 45.0%
GGATGTCCCCATACATGTAA+GGG + chr4.4:31909498-31909517 None:intergenic 45.0%
GGCATGTGTAAAGCAGATCA+CGG - chr4.4:31911583-31911602 MS.gene006628:intron 45.0%
GGGATTGGACATCCAAATGA+TGG + chr4.4:31911547-31911566 None:intergenic 45.0%
TCATTTGGATGTCCAATCCC+CGG - chr4.4:31911547-31911566 MS.gene006628:intron 45.0%
TCTGCTTTACACATGCCATG+GGG + chr4.4:31911580-31911599 None:intergenic 45.0%
TGATTCCTCGCCGTAACTAA+CGG + chr4.4:31908054-31908073 None:intergenic 45.0%
TGGAGACATCTTACCGGATT+TGG - chr4.4:31907998-31908017 MS.gene006628:intron 45.0%
TGGCGAAGGAAATTCAGTAC+CGG + chr4.4:31911652-31911671 None:intergenic 45.0%
! ACCAGTCTTTTCTGCACATG+TGG - chr4.4:31909529-31909548 MS.gene006628:intron 45.0%
! TGAGCTTAGCTCAGTTGGTA+GGG - chr4.4:31910043-31910062 MS.gene006628:intron 45.0%
!! AAATGGTGCAATGGTGCCAT+AGG + chr4.4:31909471-31909490 None:intergenic 45.0%
!! CCAGCACTTGATAGCGTAAT+GGG - chr4.4:31912018-31912037 MS.gene006628:CDS 45.0%
!! GAAGGAAATTCAGTACCGGT+GGG + chr4.4:31911648-31911667 None:intergenic 45.0%
!! TCCAGCACTTGATAGCGTAA+TGG - chr4.4:31912017-31912036 MS.gene006628:CDS 45.0%
!!! CAATTTTTTGGTGGTGTCCG+GGG + chr4.4:31910120-31910139 None:intergenic 45.0%
!! ATATTTAACTATTAAATTAA+AGG - chr4.4:31910697-31910716 MS.gene006628:intron 5.0%
!!! AAAAAAAAAAAATTTTTTGA+GGG + chr4.4:31910945-31910964 None:intergenic 5.0%
!!! TAATAATTAAGATTTTTTTT+TGG - chr4.4:31909706-31909725 MS.gene006628:intron 5.0%
AACCCTGGACCTTGCATATC+TGG + chr4.4:31910093-31910112 None:intergenic 50.0%
AGAGGGAAGTGATCAGTCCA+TGG - chr4.4:31909400-31909419 MS.gene006628:intron 50.0%
ATAAACGCAATGATCCGGCC+AGG - chr4.4:31911227-31911246 MS.gene006628:intron 50.0%
CCCATTACGCTATCAAGTGC+TGG + chr4.4:31912021-31912040 None:intergenic 50.0%
CGTCGATGGAGACATCTTAC+CGG - chr4.4:31907992-31908011 MS.gene006628:intron 50.0%
CTTTACACATGCCATGGGGT+GGG + chr4.4:31911576-31911595 None:intergenic 50.0%
GATGAATTCTTCGGCGTCAC+TGG - chr4.4:31908076-31908095 MS.gene006628:intron 50.0%
GCCACATGTGCAGAAAAGAC+TGG + chr4.4:31909533-31909552 None:intergenic 50.0%
GGAAACAATGTGTTCCTGGC+CGG + chr4.4:31911244-31911263 None:intergenic 50.0%
GGCCGGATCATTGCGTTTAT+CGG + chr4.4:31911227-31911246 None:intergenic 50.0%
GGTGGGAAACAATGTGTTCC+TGG + chr4.4:31911248-31911267 None:intergenic 50.0%
GTCCAGATATGCAAGGTCCA+GGG - chr4.4:31910088-31910107 MS.gene006628:intron 50.0%
GTGAAGCAGAAGCAGCAAGT+TGG - chr4.4:31909340-31909359 MS.gene006628:intron 50.0%
GTGAGCTTAGCTCAGTTGGT+AGG - chr4.4:31910042-31910061 MS.gene006628:intron 50.0%
GTTACCACTGTTGTCAGAGG+TGG + chr4.4:31911269-31911288 None:intergenic 50.0%
TCACTTCTTCGACTTCCCAC+CGG - chr4.4:31911630-31911649 MS.gene006628:CDS 50.0%
TGCTTGCAGTGTCACCATTG+AGG - chr4.4:31907826-31907845 MS.gene006628:CDS 50.0%
TGGGAAGTCGAAGAAGTGAC+CGG + chr4.4:31911629-31911648 None:intergenic 50.0%
! CGAAGGAAATTCAGTACCGG+TGG + chr4.4:31911649-31911668 None:intergenic 50.0%
! GAGAGCATAGACGCAGCTAT+TGG - chr4.4:31911946-31911965 MS.gene006628:CDS 50.0%
! GCTTTGAGTCCAAGTGGTAG+TGG + chr4.4:31911997-31912016 None:intergenic 50.0%
!! CAGTTGGTAGGGACAACACA+TGG - chr4.4:31910054-31910073 MS.gene006628:intron 50.0%
AAGCTGTTCGGGTCAAGGCT+CGG - chr4.4:31908154-31908173 MS.gene006628:intron 55.0%
AAGGTCCAGGGTTCAAACCC+CGG - chr4.4:31910100-31910119 MS.gene006628:intron 55.0%
AAGTGCACGAGACACTCGTC+AGG - chr4.4:31908903-31908922 MS.gene006628:intron 55.0%
ACAGCTAGGAGTGGACAAGG+CGG - chr4.4:31909008-31909027 MS.gene006628:intron 55.0%
ACCACTGTTGTCAGAGGTGG+TGG + chr4.4:31911266-31911285 None:intergenic 55.0%
ACCGATGGTGTCCATGAGAG+TGG + chr4.4:31909567-31909586 None:intergenic 55.0%
AGCTGTGGAACAGCTAGGAG+TGG - chr4.4:31908999-31909018 MS.gene006628:intron 55.0%
ATAGGAGGAGGAGGAGGATG+AGG + chr4.4:31909453-31909472 None:intergenic 55.0%
ATTAGCACCCTTCCTGCTCC+CGG - chr4.4:31911607-31911626 MS.gene006628:intron 55.0%
CCAACTGAGCTAAGCTCACG+AGG + chr4.4:31910041-31910060 None:intergenic 55.0%
CCACTGTTGTCAGAGGTGGT+GGG + chr4.4:31911265-31911284 None:intergenic 55.0%
CCATGGTTGCCACTACCACT+TGG - chr4.4:31911985-31912004 MS.gene006628:CDS 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG - chr4.4:31910038-31910057 MS.gene006628:intron 55.0%
GACTTCGGAGACCTTTCCGA+AGG - chr4.4:31907921-31907940 MS.gene006628:CDS 55.0%
GCCACTCTCATGGACACCAT+CGG - chr4.4:31909563-31909582 MS.gene006628:intron 55.0%
GCTTTACACATGCCATGGGG+TGG + chr4.4:31911577-31911596 None:intergenic 55.0%
GGAACAGCTAGGAGTGGACA+AGG - chr4.4:31909005-31909024 MS.gene006628:intron 55.0%
GGGAAGTCGAAGAAGTGACC+GGG + chr4.4:31911628-31911647 None:intergenic 55.0%
GGTCCAGATATGCAAGGTCC+AGG - chr4.4:31910087-31910106 MS.gene006628:intron 55.0%
GGTTAGAGAGAGCTACCTGC+TGG + chr4.4:31909635-31909654 None:intergenic 55.0%
GTGCAAGCTGTGGAACAGCT+AGG - chr4.4:31908994-31909013 MS.gene006628:intron 55.0%
GTTCTCCGTTAGTTACGGCG+AGG - chr4.4:31908046-31908065 MS.gene006628:intron 55.0%
TAGAGAGAGCTACCTGCTGG+TGG + chr4.4:31909632-31909651 None:intergenic 55.0%
TAGGAGGAGGAGGAGGATGA+GGG + chr4.4:31909452-31909471 None:intergenic 55.0%
TCATCCTCCTCCTCCTCCTA+TGG - chr4.4:31909452-31909471 MS.gene006628:intron 55.0%
TTGGCATTCTCACCACCAGC+AGG - chr4.4:31909617-31909636 MS.gene006628:intron 55.0%
! ATGGTGGTGTAGGGGTGAAG+AGG - chr4.4:31909382-31909401 MS.gene006628:intron 55.0%
! GAAGGATCTTGAGGTGGTGG+AGG + chr4.4:31909594-31909613 None:intergenic 55.0%
! GGCGGTGCCTTCAAGGATTT+TGG - chr4.4:31909026-31909045 MS.gene006628:intron 55.0%
! TGGTGGTGTAGGGGTGAAGA+GGG - chr4.4:31909383-31909402 MS.gene006628:intron 55.0%
!! AATGGTGCCATAGGAGGAGG+AGG + chr4.4:31909462-31909481 None:intergenic 55.0%
!! CCAAGTGGTAGTGGCAACCA+TGG + chr4.4:31911988-31912007 None:intergenic 55.0%
!! GCTGGAGCTTTGAGTCCAAG+TGG + chr4.4:31912003-31912022 None:intergenic 55.0%
!! TGCAATGGTGCCATAGGAGG+AGG + chr4.4:31909465-31909484 None:intergenic 55.0%
!! TGGTGCAATGGTGCCATAGG+AGG + chr4.4:31909468-31909487 None:intergenic 55.0%
AGAAGTGACCGGGAGCAGGA+AGG + chr4.4:31911618-31911637 None:intergenic 60.0%
AGGAGGAGGAGGAGGATGAG+GGG + chr4.4:31909451-31909470 None:intergenic 60.0%
AGGATGAGGGGGGAAACCCA+TGG + chr4.4:31909439-31909458 None:intergenic 60.0%
ATGAGGGGGGAAACCCATGG+TGG + chr4.4:31909436-31909455 None:intergenic 60.0%
CATGAGAGTGGCTGCGGTGT+AGG + chr4.4:31909555-31909574 None:intergenic 60.0%
CCCACCACCTCTGACAACAG+TGG - chr4.4:31911262-31911281 MS.gene006628:intron 60.0%
CTACACCGCAGCCACTCTCA+TGG - chr4.4:31909553-31909572 MS.gene006628:intron 60.0%
GAAGTGACCGGGAGCAGGAA+GGG + chr4.4:31911617-31911636 None:intergenic 60.0%
GGTGTCCATGAGAGTGGCTG+CGG + chr4.4:31909561-31909580 None:intergenic 60.0%
TCGAAGAAGTGACCGGGAGC+AGG + chr4.4:31911622-31911641 None:intergenic 60.0%
TGAGGGGGGAAACCCATGGT+GGG + chr4.4:31909435-31909454 None:intergenic 60.0%
TGGACAAGGCGGTGCCTTCA+AGG - chr4.4:31909019-31909038 MS.gene006628:intron 60.0%
!! AAACCCATGGTGGGTGCAGC+TGG + chr4.4:31909426-31909445 None:intergenic 60.0%
ATGCCATGGGGTGGGATGCC+GGG + chr4.4:31911568-31911587 None:intergenic 65.0%
ATGGCCAGCTGCACCCACCA+TGG - chr4.4:31909419-31909438 MS.gene006628:intron 65.0%
CATGCCATGGGGTGGGATGC+CGG + chr4.4:31911569-31911588 None:intergenic 65.0%
GAGGAGGAGGAGGATGAGGG+GGG + chr4.4:31909449-31909468 None:intergenic 65.0%
GGAGGAGGAGGAGGATGAGG+GGG + chr4.4:31909450-31909469 None:intergenic 65.0%
GGTGTCCGGGGTTTGAACCC+TGG + chr4.4:31910108-31910127 None:intergenic 65.0%
TGGCCAGCTGCACCCACCAT+GGG - chr4.4:31909420-31909439 MS.gene006628:intron 65.0%
! GGTGCCATAGGAGGAGGAGG+AGG + chr4.4:31909459-31909478 None:intergenic 65.0%
!! ATGGTGGGTGCAGCTGGCCA+TGG + chr4.4:31909420-31909439 None:intergenic 65.0%
!! GGTGGTGGAGGAGCTACCGA+TGG + chr4.4:31909582-31909601 None:intergenic 65.0%
ATCCCCGGCATCCCACCCCA+TGG - chr4.4:31911562-31911581 MS.gene006628:intron 70.0%
TGCCATGGGGTGGGATGCCG+GGG + chr4.4:31911567-31911586 None:intergenic 70.0%
TGGGGTGGGATGCCGGGGAT+TGG + chr4.4:31911562-31911581 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 31907825 31912092 31907825 ID=MS.gene006628
chr4.4 mRNA 31907825 31912092 31907825 ID=MS.gene006628.t1;Parent=MS.gene006628
chr4.4 exon 31911619 31912092 31911619 ID=MS.gene006628.t1.exon1;Parent=MS.gene006628.t1
chr4.4 CDS 31911619 31912092 31911619 ID=cds.MS.gene006628.t1;Parent=MS.gene006628.t1
chr4.4 exon 31910816 31910956 31910816 ID=MS.gene006628.t1.exon2;Parent=MS.gene006628.t1
chr4.4 CDS 31910816 31910956 31910816 ID=cds.MS.gene006628.t1;Parent=MS.gene006628.t1
chr4.4 exon 31910279 31910611 31910279 ID=MS.gene006628.t1.exon3;Parent=MS.gene006628.t1
chr4.4 CDS 31910279 31910611 31910279 ID=cds.MS.gene006628.t1;Parent=MS.gene006628.t1
chr4.4 exon 31908634 31908696 31908634 ID=MS.gene006628.t1.exon4;Parent=MS.gene006628.t1
chr4.4 CDS 31908634 31908696 31908634 ID=cds.MS.gene006628.t1;Parent=MS.gene006628.t1
chr4.4 exon 31908266 31908376 31908266 ID=MS.gene006628.t1.exon5;Parent=MS.gene006628.t1
chr4.4 CDS 31908266 31908376 31908266 ID=cds.MS.gene006628.t1;Parent=MS.gene006628.t1
chr4.4 exon 31907825 31907977 31907825 ID=MS.gene006628.t1.exon6;Parent=MS.gene006628.t1
chr4.4 CDS 31907825 31907977 31907825 ID=cds.MS.gene006628.t1;Parent=MS.gene006628.t1
Gene Sequence

>MS.gene006628

ATGCTTGCAGTGTCACCATTGAGGAACACTAACAAAGATGGAAACCAAGAAGAGATGGAGAGTTTTTCGATGGGGAGTACTACAATTACAACGGGCGACTTCGGAGACCTTTCCGAAGGAAATTTATTGGAAAGCATCAACTTCGACGACTTCTTTGTTAGCATCAACGTCGATGGAGACATCTTACCGGATTTGGTAATGGATCCAGAGATGTTTGCTGAGTTCTCCGTTAGTTACGGCGAGGAATCAGAGATGAATTCTTCGGCGTCACTGGAGAACAATAGCAAAGTAGAAGAGAAGAATTCAAAGAAAGATTATGAGGATAAAACAAGCTGTTCGGGTCAAGGCTCGGAAGAGATAGCGAGTAAAAGAGATGAATCTGTGGTTGTGAATCCATTGTTAAAAGATGCAGGTGGAAAAGGAAGAAAATCATCGAGTAAATCTAAGAATCCTCAAGCGAAGAGAAAAGTCAAGGTATACTTAAATAAGGAAACTATAGAGTTTTATTCTTGCTAAGTGGTTTATCTTAATTTGGTAATTAAATTCAGTGTACTAACTTTTTAGCTAAGGTTCTGTTACTGTCTTCTTGTTACGACCCTTGACAAATTATAGAGACAGAATAAAACATGAAAGATTGCAACTGTTCAAAATTTCGCTGGTAATTTTTTTTTTTTTTTTTATAGTGCGTTGTATGTTTGGTATTATTATGTGACTTTGTCATGACAAATTCATCGTGATATTCATACTAGCAATTATTAAAAAAATGTTAGCAGTGGTAGAGCTTCGAACTTTTTATAGCATTTTTTTTTTCGGTTTTGTTAACAATTCGAATTTACCGGTATATTCATCGTAATTAGGATCTTCATCTCTAACGAGGCAATCCAAATATCCTCACAATTTATATATAAGTAATACTGCTATTATTATTAAATAGGAAATGTTAACGATTGAACTTAAATAGCAATTCTTTAAGAAACTTAAAGAAATAAAATTTTATGAATAATTTGCATATTACATGTCATGGAAATGAAAATATTTAATTTTTTTAAGAGTAATTTCTTGATTTGAAATCCTTAAAAAGTGCACGAGACACTCGTCAGGAAGATTCTTACGTAATTAGTGTGTTTATTGTGCAGGTGGATTGGACACCAGACTTGCACAGAAGATTTGTGCAAGCTGTGGAACAGCTAGGAGTGGACAAGGCGGTGCCTTCAAGGATTTTGGAGATTATGGAAATTGATTGTCTCACTCGCCATAACATTGCTAGTCACCTTCAGGTACATATGAATTTCAAGCATATAGAAGAAAAAGGAAATTAAATTGACATTTCGTTAATTGATTGATTGTTAAGTAAGAGTACTCGTACGATGTCTTTATGGAAAGTTATTTAAATTTTCAACTTACACTATTTATCAAGTTTTGATGGAAACTTATCACGAGTTCTAGAAGATTATATTGAGCATAATTTTTTGTTATTGCAGAAATATAGGTCACATAGAAAACATTTGCTAGCGCGTGAAGCAGAAGCAGCAAGTTGGAATCAAAGGAGACAAGTATATGGTGGTGTAGGGGTGAAGAGGGAAGTGATCAGTCCATGGCCAGCTGCACCCACCATGGGTTTCCCCCCTCATCCTCCTCCTCCTCCTATGGCACCATTGCACCATTTTAGACCCTTACATGTATGGGGACATCCTTCAAATATGGACCAGTCTTTTCTGCACATGTGGCCTACACCGCAGCCACTCTCATGGACACCATCGGTAGCTCCTCCACCACCTCAAGATCCTTCATTTTGGCATTCTCACCACCAGCAGGTAGCTCTCTCTAACCACTGAAAATAGCAGCCAAATTCCCTATATATCTATCATTGTATAGTAATACTAATAATTAAGATTTTTTTTTGGACAAAAACTAACGATTAAGATTTTAAACTTTGGAAAAAGAACACCCTTAATTATTTCAGTTTGTACTCCCGCTGGTTTCATATATAAAAACAAATTATTGTTTAGGTTCATTGAATAATTAATATATCTAAACTAAAAAAAAAAATTATATATGAGATCGGAGAGAGTATTTGATAGGAAAATATACAATTTTTAGGAGGAACACTTACTTGGGCAAAGCCCAAATAACAATATTTGAGCACACACAAAACCCAAAAAAAATATTAAAAGAGAATAAACTTAAATAATATCAATTGATGTGATTTAAGTCCTCGTGAGCTTAGCTCAGTTGGTAGGGACAACACATGGTAATATGCAAGGTCCAGATATGCAAGGTCCAGGGTTCAAACCCCGGACACCACCAAAAAATTGATGTGACTTAATATTTTTTGATTAATGTTGTTAATTTCAAATCCCGGACACCACCAAAAAATTGATGTGACTTAATATTTTTTGATTAATGTTGTTAATTTCAAATTGATGTGACTTAATATTTTTTGATTAATGTTGTTAATTTCATACGAGTGTGTCCAAATAATATTTATTTAAGGTTGTGTCCAGGAAAGACTTTCTCTAATTTTTATTTTTCTCCAATTTTTATATATACCATTTGTTCGTTATATTCAAAAATTGAAGTATATTGATATACTTGTTTTTTAAATTAATAAAGTTAACGATAATTTTTATAAAGTAAAAAATTATTTGTCGACTTTTATATTTTAGGAAATATCATTTCATAAGAAAAAAATAACATGTCATTGTTTATAAGAGTCATATTAAATTTTCTACATTTTATCATAAAAAATCATCAATATTCAGTATTATGAGTAATAAAAATTTAGAAAAAAATAAATTTTATTTTTCCCAAATGATATTTAGTTATTATAACTGTAAATGAAAGAAAATGATTTTTTTAAAAAAAATAATACATATTTAACTATTAAATTAAAGGCCAAACGTACTTGTACATATCGAATTTTCGAGTTAACAGTAAAAATTGTCATATATTATATATTTAACTACATATTATTTGTCATATATTATATATAAGGCATGAGAGAGTTTCTTGTAGCTAGTGATAATTTTGCATCCTAGAAAAAGATTTTAAAACAATTGCTATAATAATTAAACTATTTTTGATAATTTACCAACCACATATAATTGAATCCTTGTTCCCTCAAAAAATTTTTTTTTTTTGAATCCTCGTCAAAAAAGAAGAAGAACTATGTACATATAATTCAATGATGTTATTTTCAAAAAATAAAATAAAAATTCAATGATGCTGTGTGAAAATCTTGTTTGTTATAAATAAAAATTCGAGGATACATATTAATAACTATACAAATGTTTACAACAAAAATATATTAAAATTAGATCTTGATTAAAATTCACGTTAATTCTTTGATTCGTGTATCTAAAATGTGATGTATTTCACTATTTCTATGATCAACAGACTCCGATAAACGCAATGATCCGGCCAGGAACACATTGTTTCCCACCACCTCTGACAACAGTGGTAACTATTCATTACATCACTTTACTATTTTGGAGCCATGCACGTTGTCTTCTACTCTGAATTTTGGATCCTTTTTAAAGATAAGATTGACAGCATACAAATTTTAGTATTGTCATATTTACCAACATATATAAATATAGCACATAATTATATATGTTGTCGTATATATATTTAGTCACAAACACATACACAAATTATTCTCCAAATATAAGTTTGGCTGACCATAAAGGAAAATAACATAAGAACATAACATGCAGAGACCATCATTTGGATGTCCAATCCCCGGCATCCCACCCCATGGCATGTGTAAAGCAGATCACGGCATTAGCACCCTTCCTGCTCCCGGTCACTTCTTCGACTTCCCACCGGTACTGAATTTCCTTCGCCATACATACATACATGCATTCAACATCTTTTTTTCTTCAATTCTCTTTATTTAGTTTCCGGCCATATATTAAAAAGTTTTACATTATATGAGAGACGATAAGGGCATGTGTTTATAAGGTGGGAGTCATTGCTCACTTTATAAGACGATTTTTTAACGATGAGTTAACCCCAATATAAAAACACCTAAAAGCTCCATAGATTTCAAGGCATGAGTAATTAGATGATTCGTTGTTTGTATGCTAATGTATTTTTATAAATTTATTTTACAGTCAAATGAGAGCATAGACGCAGCTATTGGAGATGTTTTATCAAAACCATGGTTGCCACTACCACTTGGACTCAAAGCTCCAGCACTTGATAGCGTAATGGGTGAACTACAAAGACAAGGAATTTCAAACATTCCACCCTCATCATGCACTTGA
Protein sequence

>MS.gene006628.t1

MLAVSPLRNTNKDGNQEEMESFSMGSTTITTGDFGDLSEGNLLESINFDDFFVSINVDGDILPDLVMDPEMFAEFSVSYGEESEMNSSASLENNSKVEEKNSKKDYEDKTSCSGQGSEEIASKRDESVVVNPLLKDAGGKGRKSSSKSKNPQAKRKVKVDWTPDLHRRFVQAVEQLGVDKAVPSRILEIMEIDCLTRHNIASHLQKYRSHRKHLLAREAEAASWNQRRQVYGGVGVKREVISPWPAAPTMGFPPHPPPPPMAPLHHFRPLHVWGHPSNMDQSFLHMWPTPQPLSWTPSVAPPPPQDPSFWHSHHQQTPINAMIRPGTHCFPPPLTTVRPSFGCPIPGIPPHGMCKADHGISTLPAPGHFFDFPPSNESIDAAIGDVLSKPWLPLPLGLKAPALDSVMGELQRQGISNIPPSSCT