Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006872.t1 | AES90030.2 | 87.2 | 156 | 20 | 0 | 10 | 165 | 38 | 193 | 4.50E-71 | 277.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006872.t1 | Q7XLP4 | 39.1 | 110 | 63 | 2 | 37 | 143 | 83 | 191 | 9.4e-17 | 88.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006872.t1 | G7JHL3 | 87.2 | 156 | 20 | 0 | 10 | 165 | 38 | 193 | 3.3e-71 | 277.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene006872.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006872.t1 | MTR_4g083390 | 87.179 | 156 | 20 | 0 | 10 | 165 | 38 | 193 | 1.47e-98 | 282 |
| MS.gene006872.t1 | MTR_4g083390 | 90.977 | 133 | 12 | 0 | 33 | 165 | 1 | 133 | 4.21e-88 | 253 |
| MS.gene006872.t1 | MTR_4g083390 | 85.496 | 131 | 19 | 0 | 33 | 163 | 1 | 131 | 2.68e-80 | 234 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGAATTGAACCGCTTTC+AGG | 0.230161 | 4.4:+29605210 | MS.gene006872:CDS |
| TTCAAAGTTCAAATTCTTAG+AGG | 0.303256 | 4.4:+29605615 | MS.gene006872:CDS |
| AGTTGGGCCACGACTTATAA+TGG | 0.308961 | 4.4:+29605471 | MS.gene006872:CDS |
| ACGGGGACTAAGATGTGTTT+TGG | 0.312922 | 4.4:-29605259 | None:intergenic |
| TTCCGACTATTCTCAAATAT+CGG | 0.320685 | 4.4:+29605442 | MS.gene006872:CDS |
| GCATCCCTCTCACCGGTTCC+AGG | 0.356774 | 4.4:-29605669 | None:intergenic |
| TGAAATTACCAATCCATAAC+GGG | 0.384987 | 4.4:-29605277 | None:intergenic |
| GAGAATAGTCGGAACCGATT+TGG | 0.387243 | 4.4:-29605433 | None:intergenic |
| ACACATCTTAGTCCCCGTTA+TGG | 0.404304 | 4.4:+29605264 | MS.gene006872:CDS |
| GAAGATAATTATTTGAAGCT+TGG | 0.405476 | 4.4:+29605543 | MS.gene006872:CDS |
| TCCGACTATTCTCAAATATC+GGG | 0.408506 | 4.4:+29605443 | MS.gene006872:CDS |
| GCCAACGCTATCAAACAGTT+TGG | 0.414501 | 4.4:-29605505 | None:intergenic |
| AACTGTTTGATAGCGTTGGC+TGG | 0.415821 | 4.4:+29605508 | MS.gene006872:CDS |
| CTTTACGAATTTCCTGGAAC+CGG | 0.450094 | 4.4:+29605657 | MS.gene006872:CDS |
| CAAATATCGGGGTAAGAGTT+GGG | 0.464634 | 4.4:+29605455 | MS.gene006872:CDS |
| GAAAGGACGACATGAAAGTA+TGG | 0.466808 | 4.4:-29605237 | None:intergenic |
| GATTACTAATCTATGACAAA+TGG | 0.467072 | 4.4:-29605693 | None:intergenic |
| TCAAATATCGGGGTAAGAGT+TGG | 0.468409 | 4.4:+29605454 | MS.gene006872:CDS |
| TGAAGAACTTCCTTCCAAAT+CGG | 0.469161 | 4.4:+29605419 | MS.gene006872:CDS |
| GTTGGGCCACGACTTATAAT+GGG | 0.482324 | 4.4:+29605472 | MS.gene006872:CDS |
| ATGAAATTACCAATCCATAA+CGG | 0.499697 | 4.4:-29605278 | None:intergenic |
| ATGTCGTCTTCAAAATCAGC+TGG | 0.502794 | 4.4:+29605016 | MS.gene006872:CDS |
| ACCAAACTGTTTGATAGCGT+TGG | 0.508522 | 4.4:+29605504 | MS.gene006872:CDS |
| CCCCGATATTTGAGAATAGT+CGG | 0.515602 | 4.4:-29605444 | None:intergenic |
| TCTTAGTCCCCGTTATGGAT+TGG | 0.515821 | 4.4:+29605269 | MS.gene006872:CDS |
| TCCTTCGACAAATATGCAAA+CGG | 0.531644 | 4.4:+29605188 | MS.gene006872:CDS |
| GAAGGTGATGATAACGAGGC+TGG | 0.531657 | 4.4:-29605162 | None:intergenic |
| ACTGTTTGATAGCGTTGGCT+GGG | 0.539366 | 4.4:+29605509 | MS.gene006872:CDS |
| TCCGTTTGCATATTTGTCGA+AGG | 0.549975 | 4.4:-29605189 | None:intergenic |
| GTTCTGCCCATTATAAGTCG+TGG | 0.557426 | 4.4:-29605478 | None:intergenic |
| TCTTCAAAATCAGCTGGAAG+AGG | 0.564849 | 4.4:+29605022 | MS.gene006872:CDS |
| AAATGGTGCATCCCTCTCAC+CGG | 0.578086 | 4.4:-29605676 | None:intergenic |
| GAGTAGCAGTCAAGTTGCTG+TGG | 0.583739 | 4.4:+29605134 | MS.gene006872:CDS |
| TCTTCACATATGCTGCTGCT+CGG | 0.585483 | 4.4:-29605402 | None:intergenic |
| ATTTCCTGGAACCGGTGAGA+GGG | 0.587927 | 4.4:+29605665 | MS.gene006872:CDS |
| TCGACAAATATGCAAACGGA+TGG | 0.588200 | 4.4:+29605192 | MS.gene006872:CDS |
| AGTAGCAGTCAAGTTGCTGT+GGG | 0.603556 | 4.4:+29605135 | MS.gene006872:CDS |
| ATATTTGTCGAAGGAGCAGA+AGG | 0.614477 | 4.4:-29605180 | None:intergenic |
| AATTTCCTGGAACCGGTGAG+AGG | 0.633829 | 4.4:+29605664 | MS.gene006872:CDS |
| ATAGTCGGAACCGATTTGGA+AGG | 0.643344 | 4.4:-29605429 | None:intergenic |
| AAGTATGGCACTCCTGAAAG+CGG | 0.678549 | 4.4:-29605222 | None:intergenic |
| CCGACTATTCTCAAATATCG+GGG | 0.681942 | 4.4:+29605444 | MS.gene006872:CDS |
| AGCAGAAGGTGATGATAACG+AGG | 0.685363 | 4.4:-29605166 | None:intergenic |
| GAAATTACCAATCCATAACG+GGG | 0.716232 | 4.4:-29605276 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATCTTGTATTGTATTGT+TGG | + | chr4.4:29605369-29605388 | MS.gene006872:intron | 20.0% |
| !! | TAATGTGATATGAAATACTA+AGG | - | chr4.4:29605055-29605074 | None:intergenic | 20.0% |
| !! | TTATAGATCAAACTAAATGA+AGG | + | chr4.4:29605318-29605337 | MS.gene006872:intron | 20.0% |
| ! | AAGATTAACTTTCATGTACA+CGG | + | chr4.4:29605077-29605096 | MS.gene006872:intron | 25.0% |
| ! | ATGAAATTACCAATCCATAA+CGG | - | chr4.4:29605281-29605300 | None:intergenic | 25.0% |
| ! | TATTGTATTGTTGGCATATA+GGG | + | chr4.4:29605378-29605397 | MS.gene006872:intron | 25.0% |
| ! | TTCAAAGTTCAAATTCTTAG+AGG | + | chr4.4:29605615-29605634 | MS.gene006872:CDS | 25.0% |
| !! | ATCAAACTAAATGAAGGTTT+TGG | + | chr4.4:29605324-29605343 | MS.gene006872:intron | 25.0% |
| !! | GAAGATAATTATTTGAAGCT+TGG | + | chr4.4:29605543-29605562 | MS.gene006872:CDS | 25.0% |
| ATTGTATTGTTGGCATATAG+GGG | + | chr4.4:29605379-29605398 | MS.gene006872:intron | 30.0% | |
| GGAAATTCGTAAAGCAAAAA+TGG | - | chr4.4:29605651-29605670 | None:intergenic | 30.0% | |
| GTATTGTATTGTTGGCATAT+AGG | + | chr4.4:29605377-29605396 | MS.gene006872:intron | 30.0% | |
| GTGTACATGAAAGTTAATCT+TGG | - | chr4.4:29605078-29605097 | None:intergenic | 30.0% | |
| TGAAATTACCAATCCATAAC+GGG | - | chr4.4:29605280-29605299 | None:intergenic | 30.0% | |
| TTCCGACTATTCTCAAATAT+CGG | + | chr4.4:29605442-29605461 | MS.gene006872:CDS | 30.0% | |
| ! | TTTTTGCTTTACGAATTTCC+TGG | + | chr4.4:29605651-29605670 | MS.gene006872:CDS | 30.0% |
| !!! | ACTAAATGAAGGTTTTGGAA+AGG | + | chr4.4:29605329-29605348 | MS.gene006872:intron | 30.0% |
| GAAATTACCAATCCATAACG+GGG | - | chr4.4:29605279-29605298 | None:intergenic | 35.0% | |
| TCCGACTATTCTCAAATATC+GGG | + | chr4.4:29605443-29605462 | MS.gene006872:CDS | 35.0% | |
| TCCTTCGACAAATATGCAAA+CGG | + | chr4.4:29605188-29605207 | MS.gene006872:CDS | 35.0% | |
| TGAAGAACTTCCTTCCAAAT+CGG | + | chr4.4:29605419-29605438 | MS.gene006872:CDS | 35.0% | |
| TTAACTTTCATGTACACGGT+CGG | + | chr4.4:29605081-29605100 | MS.gene006872:intron | 35.0% | |
| !!! | GACTAAGATGTGTTTTGGAA+AGG | - | chr4.4:29605257-29605276 | None:intergenic | 35.0% |
| ATATTTGTCGAAGGAGCAGA+AGG | - | chr4.4:29605183-29605202 | None:intergenic | 40.0% | |
| ATGTCGTCTTCAAAATCAGC+TGG | + | chr4.4:29605016-29605035 | MS.gene006872:CDS | 40.0% | |
| CAAATATCGGGGTAAGAGTT+GGG | + | chr4.4:29605455-29605474 | MS.gene006872:CDS | 40.0% | |
| CCCCGATATTTGAGAATAGT+CGG | - | chr4.4:29605447-29605466 | None:intergenic | 40.0% | |
| CCGACTATTCTCAAATATCG+GGG | + | chr4.4:29605444-29605463 | MS.gene006872:CDS | 40.0% | |
| CTTTACGAATTTCCTGGAAC+CGG | + | chr4.4:29605657-29605676 | MS.gene006872:CDS | 40.0% | |
| GAAAGGACGACATGAAAGTA+TGG | - | chr4.4:29605240-29605259 | None:intergenic | 40.0% | |
| GCTCATGAAAAACAGTGAAG+AGG | + | chr4.4:29605584-29605603 | MS.gene006872:CDS | 40.0% | |
| TCAAATATCGGGGTAAGAGT+TGG | + | chr4.4:29605454-29605473 | MS.gene006872:CDS | 40.0% | |
| TCCGTTTGCATATTTGTCGA+AGG | - | chr4.4:29605192-29605211 | None:intergenic | 40.0% | |
| TCGACAAATATGCAAACGGA+TGG | + | chr4.4:29605192-29605211 | MS.gene006872:CDS | 40.0% | |
| TCTTCAAAATCAGCTGGAAG+AGG | + | chr4.4:29605022-29605041 | MS.gene006872:CDS | 40.0% | |
| !! | ACCAAACTGTTTGATAGCGT+TGG | + | chr4.4:29605504-29605523 | MS.gene006872:CDS | 40.0% |
| AAGTATGGCACTCCTGAAAG+CGG | - | chr4.4:29605225-29605244 | None:intergenic | 45.0% | |
| ACACATCTTAGTCCCCGTTA+TGG | + | chr4.4:29605264-29605283 | MS.gene006872:CDS | 45.0% | |
| AGTTGGGCCACGACTTATAA+TGG | + | chr4.4:29605471-29605490 | MS.gene006872:CDS | 45.0% | |
| GAGAATAGTCGGAACCGATT+TGG | - | chr4.4:29605436-29605455 | None:intergenic | 45.0% | |
| GATGGAATTGAACCGCTTTC+AGG | + | chr4.4:29605210-29605229 | MS.gene006872:CDS | 45.0% | |
| GCCAACGCTATCAAACAGTT+TGG | - | chr4.4:29605508-29605527 | None:intergenic | 45.0% | |
| GTTCTGCCCATTATAAGTCG+TGG | - | chr4.4:29605481-29605500 | None:intergenic | 45.0% | |
| GTTGGGCCACGACTTATAAT+GGG | + | chr4.4:29605472-29605491 | MS.gene006872:CDS | 45.0% | |
| TCTTAGTCCCCGTTATGGAT+TGG | + | chr4.4:29605269-29605288 | MS.gene006872:CDS | 45.0% | |
| TCTTCACATATGCTGCTGCT+CGG | - | chr4.4:29605405-29605424 | None:intergenic | 45.0% | |
| ! | ACGGGGACTAAGATGTGTTT+TGG | - | chr4.4:29605262-29605281 | None:intergenic | 45.0% |
| ! | AGCAGAAGGTGATGATAACG+AGG | - | chr4.4:29605169-29605188 | None:intergenic | 45.0% |
| ! | AGTAGCAGTCAAGTTGCTGT+GGG | + | chr4.4:29605135-29605154 | MS.gene006872:CDS | 45.0% |
| !! | AACTGTTTGATAGCGTTGGC+TGG | + | chr4.4:29605508-29605527 | MS.gene006872:CDS | 45.0% |
| !! | ACTGTTTGATAGCGTTGGCT+GGG | + | chr4.4:29605509-29605528 | MS.gene006872:CDS | 45.0% |
| !! | ATAGTCGGAACCGATTTGGA+AGG | - | chr4.4:29605432-29605451 | None:intergenic | 45.0% |
| AAATGGTGCATCCCTCTCAC+CGG | - | chr4.4:29605679-29605698 | None:intergenic | 50.0% | |
| AATTTCCTGGAACCGGTGAG+AGG | + | chr4.4:29605664-29605683 | MS.gene006872:CDS | 50.0% | |
| ATTTCCTGGAACCGGTGAGA+GGG | + | chr4.4:29605665-29605684 | MS.gene006872:CDS | 50.0% | |
| ! | GAAGGTGATGATAACGAGGC+TGG | - | chr4.4:29605165-29605184 | None:intergenic | 50.0% |
| ! | GAGTAGCAGTCAAGTTGCTG+TGG | + | chr4.4:29605134-29605153 | MS.gene006872:CDS | 50.0% |
| !! | GCATCCCTCTCACCGGTTCC+AGG | - | chr4.4:29605672-29605691 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29605016 | 29605710 | 29605016 | ID=MS.gene006872 |
| chr4.4 | mRNA | 29605016 | 29605710 | 29605016 | ID=MS.gene006872.t1;Parent=MS.gene006872 |
| chr4.4 | exon | 29605016 | 29605043 | 29605016 | ID=MS.gene006872.t1.exon1;Parent=MS.gene006872.t1 |
| chr4.4 | CDS | 29605016 | 29605043 | 29605016 | ID=cds.MS.gene006872.t1;Parent=MS.gene006872.t1 |
| chr4.4 | exon | 29605133 | 29605290 | 29605133 | ID=MS.gene006872.t1.exon2;Parent=MS.gene006872.t1 |
| chr4.4 | CDS | 29605133 | 29605290 | 29605133 | ID=cds.MS.gene006872.t1;Parent=MS.gene006872.t1 |
| chr4.4 | exon | 29605399 | 29605710 | 29605399 | ID=MS.gene006872.t1.exon3;Parent=MS.gene006872.t1 |
| chr4.4 | CDS | 29605399 | 29605710 | 29605399 | ID=cds.MS.gene006872.t1;Parent=MS.gene006872.t1 |
| Gene Sequence |
| Protein sequence |
