AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006933

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006933.t1 MTR_4g084550 90.761 184 7 3 1 182 1 176 2.55e-120 338
MS.gene006933.t1 MTR_8g107210 58.333 120 41 3 65 184 94 204 9.76e-36 128
MS.gene006933.t1 MTR_7g060630 71.429 70 20 0 60 129 1 70 6.75e-31 112
MS.gene006933.t1 MTR_5g021930 67.164 67 21 1 63 128 26 92 5.66e-27 103
MS.gene006933.t1 MTR_5g081990 61.765 68 26 0 67 134 33 100 7.15e-27 100
MS.gene006933.t1 MTR_1g019130 68.750 64 20 0 66 129 89 152 1.37e-26 101
MS.gene006933.t1 MTR_1g019130 68.750 64 20 0 66 129 89 152 2.75e-26 100
MS.gene006933.t1 MTR_3g088485 84.615 52 8 0 78 129 91 142 4.03e-26 98.2
MS.gene006933.t1 MTR_8g095580 84.615 52 8 0 78 129 93 144 4.11e-26 98.2
MS.gene006933.t1 MTR_4g063735 66.667 63 21 0 67 129 25 87 1.75e-25 99.0
MS.gene006933.t1 MTR_7g025010 46.377 69 37 0 62 130 42 110 2.49e-18 82.0
MS.gene006933.t1 MTR_1g115315 46.341 82 38 1 67 148 85 160 1.32e-17 78.2
MS.gene006933.t1 MTR_2g015000 44.928 69 38 0 62 130 50 118 1.57e-17 79.7
MS.gene006933.t1 MTR_1g115315 46.341 82 38 1 67 148 85 160 1.65e-17 78.2
MS.gene006933.t1 MTR_4g088070 44.776 67 37 0 61 127 23 89 2.10e-16 76.3
MS.gene006933.t1 MTR_4g088080 38.356 73 45 0 55 127 28 100 7.37e-14 68.6
MS.gene006933.t1 MTR_3g115620 42.623 61 35 0 67 127 82 142 2.03e-13 66.2
MS.gene006933.t1 MTR_6g027390 39.474 76 46 0 66 141 31 106 5.46e-13 66.2
MS.gene006933.t1 MTR_7g023810 32.743 113 66 1 47 159 48 150 1.05e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006933.t1 AT3G18010 55.285 123 41 4 65 182 74 187 5.89e-36 128
MS.gene006933.t1 AT2G28610 65.714 70 24 0 60 129 1 70 3.34e-29 108
MS.gene006933.t1 AT2G01500 51.485 101 42 2 27 121 16 115 1.03e-27 105
MS.gene006933.t1 AT5G59340 64.706 68 24 0 65 132 12 79 3.12e-27 103
MS.gene006933.t1 AT3G11260 44.444 108 50 1 54 151 11 118 1.79e-26 99.8
MS.gene006933.t1 AT2G17950 60.759 79 30 1 63 140 34 112 6.77e-26 100
MS.gene006933.t1 AT1G46480 63.768 69 24 1 61 129 85 152 7.47e-26 98.6
MS.gene006933.t1 AT1G46480 63.768 69 24 1 61 129 85 152 4.73e-25 97.8
MS.gene006933.t1 AT5G05770 59.375 64 26 0 67 130 29 92 7.10e-23 89.0
MS.gene006933.t1 AT2G33880 36.842 114 71 1 18 130 5 118 8.58e-18 80.1
MS.gene006933.t1 AT2G33880 36.842 114 71 1 18 130 5 118 1.15e-17 79.7
MS.gene006933.t1 AT5G45980 30.120 166 84 5 38 185 17 168 1.45e-16 76.3
MS.gene006933.t1 AT4G35550 49.180 61 31 0 67 127 99 159 1.67e-15 72.8
MS.gene006933.t1 AT1G20700 45.000 60 33 0 67 126 33 92 5.70e-14 66.2
MS.gene006933.t1 AT1G20700 45.000 60 33 0 67 126 95 154 1.70e-13 66.2
MS.gene006933.t1 AT1G20700 45.000 60 33 0 67 126 95 154 2.03e-13 66.2
MS.gene006933.t1 AT3G03660 49.057 53 27 0 66 118 32 84 7.28e-13 65.5
MS.gene006933.t1 AT3G03660 49.057 53 27 0 66 118 32 84 1.08e-12 65.1
MS.gene006933.t1 AT3G03660 49.057 53 27 0 66 118 32 84 1.61e-12 64.7
MS.gene006933.t1 AT3G03660 49.057 53 27 0 66 118 32 84 2.16e-12 64.7
MS.gene006933.t1 AT5G17810 43.396 53 30 0 66 118 20 72 3.61e-11 60.8
MS.gene006933.t1 AT5G17810 43.396 53 30 0 66 118 20 72 3.61e-11 60.8
MS.gene006933.t1 AT5G17810 43.396 53 30 0 66 118 20 72 3.62e-11 60.8

Find 45 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTCTCGCCTTGTGATTC+TGG 0.194023 4.3:+27489466 None:intergenic
GCTTCGCAGCTTCGACAATT+TGG 0.206061 4.3:-27489522 MS.gene006933:CDS
TGGGAATTATGGTTGGAATT+TGG 0.252701 4.3:+27490456 None:intergenic
TTGAAGGTGCATGGGAATTA+TGG 0.269434 4.3:+27490445 None:intergenic
AGAGAAAGAGAAGTACGTTA+TGG 0.359866 4.3:-27489379 MS.gene006933:CDS
CGACAATTTGGAAAGATTGA+AGG 0.396848 4.3:-27489510 MS.gene006933:CDS
GAGAAAGAGAAGTACGTTAT+GGG 0.401887 4.3:-27489378 MS.gene006933:CDS
CAATGGGTGCAAATACAATA+AGG 0.401985 4.3:+27490402 None:intergenic
GAAGCTGCGAAGCTATTTGT+TGG 0.428703 4.3:+27489532 None:intergenic
CTTGCCTATACAATTCTTCA+AGG 0.428836 4.3:+27489583 None:intergenic
TAGTCCTTGAAGAATTGTAT+AGG 0.431151 4.3:-27489587 MS.gene006933:CDS
GTTGGACTCCACCTTGAACT+TGG 0.444756 4.3:+27489624 None:intergenic
AATACAATAAGGTTGGACTA+TGG 0.444802 4.3:+27490413 None:intergenic
CTTGAGATGGAGGAAACAAT+TGG 0.464032 4.3:-27489426 MS.gene006933:CDS
TGCATCCTCAACCAAGTTCA+AGG 0.472729 4.3:-27489635 MS.gene006933:CDS
CTCCACCTTGAACTTGGTTG+AGG 0.502185 4.3:+27489630 None:intergenic
TGTTGGATTTGTTCAGCTGA+TGG 0.503667 4.3:+27489549 None:intergenic
TTGTTCACTGCAGTTTGAAG+TGG 0.506780 4.3:+27488801 None:intergenic
TCAAGGACTAGTAATTGAAC+TGG 0.509163 4.3:+27489600 None:intergenic
CTTACCGAGATGGTGATTGA+AGG 0.526602 4.3:+27490365 None:intergenic
GAAGCGTCGTCGCCTTGAGA+TGG 0.530642 4.3:-27489439 MS.gene006933:CDS
TTAAGCTTGGCTTACCGAGA+TGG 0.532231 4.3:+27490355 None:intergenic
ACTAGTAATTGAACTGGTGT+TGG 0.537149 4.3:+27489606 None:intergenic
CTTGAAGAATTGTATAGGCA+AGG 0.540070 4.3:-27489582 MS.gene006933:CDS
TCAGAAAAGCAAGAAGCTGC+AGG 0.540109 4.3:-27489351 MS.gene006933:intron
AGGGGATGAGATGGTTGCAA+TGG 0.545416 4.3:+27490385 None:intergenic
CAATTGTTTCCTCCATCTCA+AGG 0.548253 4.3:+27489427 None:intergenic
GGGTGCAAATACAATAAGGT+TGG 0.557080 4.3:+27490406 None:intergenic
TTACCGAGATGGTGATTGAA+GGG 0.563954 4.3:+27490366 None:intergenic
GGGGATGAGATGGTTGCAAT+GGG 0.571604 4.3:+27490386 None:intergenic
ATCCCCTTCAATCACCATCT+CGG 0.572975 4.3:-27490369 MS.gene006933:intron
AGGTGCATGGGAATTATGGT+TGG 0.574486 4.3:+27490449 None:intergenic
GCGTCGTCGCCTTGAGATGG+AGG 0.576407 4.3:-27489436 MS.gene006933:CDS
ATAAGGTTGGACTATGGAGG+AGG 0.582667 4.3:+27490419 None:intergenic
GGTGATTGAAGGGGATGAGA+TGG 0.585438 4.3:+27490376 None:intergenic
TTGAAGAATTGTATAGGCAA+GGG 0.590766 4.3:-27489581 MS.gene006933:CDS
ACAGCTCATGCAGCTGAGCA+AGG 0.595931 4.3:-27489672 MS.gene006933:intron
ACAATAAGGTTGGACTATGG+AGG 0.614353 4.3:+27490416 None:intergenic
AAACTGCAGTGAACAAGCAG+AGG 0.632631 4.3:-27488794 MS.gene006933:intron
ATTATGGTTGGAATTTGGAG+TGG 0.636820 4.3:+27490461 None:intergenic
TACCGAGATGGTGATTGAAG+GGG 0.640386 4.3:+27490367 None:intergenic
AGTTAAAGAGACAAAGAAGT+GGG 0.667079 4.3:-27488827 MS.gene006933:CDS
CTATTGGTTCCAGAATCACA+AGG 0.685006 4.3:-27489475 MS.gene006933:CDS
GAGTTAAAGAGACAAAGAAG+TGG 0.689037 4.3:-27488828 MS.gene006933:CDS
ATCCTCAACCAAGTTCAAGG+TGG 0.762805 4.3:-27489632 MS.gene006933:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTTCTTTCAAATA+TGG + chr4.3:27489941-27489960 None:intergenic 10.0%
!!! TAAGTTTTTATTTTTGTTTT+AGG - chr4.3:27488696-27488715 MS.gene006933:intron 10.0%
!!! AAATTATTTTAGTGTATGTA+TGG + chr4.3:27489042-27489061 None:intergenic 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG - chr4.3:27489993-27490012 MS.gene006933:intron 15.0%
!!! TTGTTGTTTATTCAATTTAA+TGG - chr4.3:27489962-27489981 MS.gene006933:intron 15.0%
!! AAAAGATTTCATAATCAAAG+AGG - chr4.3:27490246-27490265 MS.gene006933:intron 20.0%
!! AGAAAAAAAAAATGACCAAA+TGG + chr4.3:27489801-27489820 None:intergenic 20.0%
!! GAAAAAAAAAATGACCAAAT+GGG + chr4.3:27489800-27489819 None:intergenic 20.0%
!! TCATGAGTGAAATATAATAT+AGG + chr4.3:27489678-27489697 None:intergenic 20.0%
!! TTTGAGCTATATATAAACAA+CGG - chr4.3:27489065-27489084 MS.gene006933:intron 20.0%
!!! AAAGATAAATAGCTTTTGTA+AGG - chr4.3:27489734-27489753 MS.gene006933:intron 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG + chr4.3:27489917-27489936 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG + chr4.3:27489834-27489853 None:intergenic 20.0%
!!! CTTTGATTATGAAATCTTTT+GGG + chr4.3:27490246-27490265 None:intergenic 20.0%
!!! GTTAAAGTGTTAAAATTAGT+AGG + chr4.3:27488749-27488768 None:intergenic 20.0%
!!! GTTTTTTTTAACTTTCTGTT+GGG - chr4.3:27490306-27490325 MS.gene006933:intron 20.0%
!!! TCTTTGATTATGAAATCTTT+TGG + chr4.3:27490247-27490266 None:intergenic 20.0%
!!! TGTTTTTTTTAACTTTCTGT+TGG - chr4.3:27490305-27490324 MS.gene006933:intron 20.0%
!!! TTTTTTTTAACTTTCTGTTG+GGG - chr4.3:27490307-27490326 MS.gene006933:intron 20.0%
! AAATATCAATTTAGCCCATT+TGG - chr4.3:27489783-27489802 MS.gene006933:intron 25.0%
! AAGGAAAAAATGTGTTCTAT+TGG - chr4.3:27489454-27489473 MS.gene006933:CDS 25.0%
! ACAGCTAGATATTATCTTTA+GGG - chr4.3:27489620-27489639 MS.gene006933:CDS 25.0%
! AGAAAATAGTTTAGCTACTT+AGG + chr4.3:27489192-27489211 None:intergenic 25.0%
! AGAGAAGATTATACTATGAT+GGG + chr4.3:27488941-27488960 None:intergenic 25.0%
! AGTAGGTTTGCATTAAAAAA+TGG + chr4.3:27488732-27488751 None:intergenic 25.0%
! AGTATTGTTAGTATCATAGT+CGG - chr4.3:27488839-27488858 MS.gene006933:intron 25.0%
! AGTTAAAAAGTGGAATGATA+AGG + chr4.3:27488887-27488906 None:intergenic 25.0%
! ATCATATGACTGTATTTATC+TGG + chr4.3:27489110-27489129 None:intergenic 25.0%
! ATTGGTAAGGAATCTATTAA+TGG - chr4.3:27488650-27488669 MS.gene006933:intron 25.0%
! CATATGAGAAAGTTAAAAAG+TGG + chr4.3:27488897-27488916 None:intergenic 25.0%
! GAAAATAGTTTAGCTACTTA+GGG + chr4.3:27489191-27489210 None:intergenic 25.0%
! TACAGCTAGATATTATCTTT+AGG - chr4.3:27489619-27489638 MS.gene006933:CDS 25.0%
! TATGAGTAACTATATTCATG+TGG - chr4.3:27488970-27488989 MS.gene006933:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG - chr4.3:27489886-27489905 MS.gene006933:intron 25.0%
! TTTATAGAAAGAATGACAGA+GGG + chr4.3:27490439-27490458 None:intergenic 25.0%
!! GTTAAAAAAAACAGCTTTAG+AGG + chr4.3:27490298-27490317 None:intergenic 25.0%
!! TACTATATGAGTTGAATTTG+TGG - chr4.3:27490065-27490084 MS.gene006933:intron 25.0%
!! TGTTACATTTCAGATTTTCT+TGG - chr4.3:27490338-27490357 MS.gene006933:intron 25.0%
!! TTTTATAGAAAGAATGACAG+AGG + chr4.3:27490440-27490459 None:intergenic 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG - chr4.3:27489630-27489649 MS.gene006933:CDS 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG + chr4.3:27489835-27489854 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG - chr4.3:27489832-27489851 MS.gene006933:intron 25.0%
AATACAATAAGGTTGGACTA+TGG + chr4.3:27488535-27488554 None:intergenic 30.0%
ACACACACTTCAAATTGTAA+GGG + chr4.3:27490032-27490051 None:intergenic 30.0%
ACCCTTAAGAAAATTAAGCT+TGG + chr4.3:27488606-27488625 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG - chr4.3:27490118-27490137 MS.gene006933:intron 30.0%
ATTTCTCTTGTCTTTGTTGA+TGG - chr4.3:27490180-27490199 MS.gene006933:intron 30.0%
GAGAGAAGATTATACTATGA+TGG + chr4.3:27488942-27488961 None:intergenic 30.0%
GTATTGTTAGTATCATAGTC+GGG - chr4.3:27488840-27488859 MS.gene006933:intron 30.0%
TAGTCCTTGAAGAATTGTAT+AGG - chr4.3:27489358-27489377 MS.gene006933:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG - chr4.3:27489364-27489383 MS.gene006933:CDS 30.0%
! AATAGCTTTTGTAAGGATCT+CGG - chr4.3:27489741-27489760 MS.gene006933:intron 30.0%
! AGCCAAGCTTAATTTTCTTA+AGG - chr4.3:27488601-27488620 MS.gene006933:intron 30.0%
! GCCAAGCTTAATTTTCTTAA+GGG - chr4.3:27488602-27488621 MS.gene006933:intron 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG - chr4.3:27489642-27489661 MS.gene006933:CDS 30.0%
!! ATCAAAGAGGTTTTGTATGT+TGG - chr4.3:27490259-27490278 MS.gene006933:intron 30.0%
!! TGAAATCTTTTGGGTATCTT+TGG + chr4.3:27490237-27490256 None:intergenic 30.0%
ACTAGTAATTGAACTGGTGT+TGG + chr4.3:27489342-27489361 None:intergenic 35.0%
ATTATGGTTGGAATTTGGAG+TGG + chr4.3:27488487-27488506 None:intergenic 35.0%
ATTCCTTACCAATGCCTTTA+GGG + chr4.3:27488643-27488662 None:intergenic 35.0%
CAATGGGTGCAAATACAATA+AGG + chr4.3:27488546-27488565 None:intergenic 35.0%
CACACACACTTCAAATTGTA+AGG + chr4.3:27490033-27490052 None:intergenic 35.0%
CGACAATTTGGAAAGATTGA+AGG - chr4.3:27489435-27489454 MS.gene006933:CDS 35.0%
CTTGAAGAATTGTATAGGCA+AGG - chr4.3:27489363-27489382 MS.gene006933:CDS 35.0%
CTTGCCTATACAATTCTTCA+AGG + chr4.3:27489365-27489384 None:intergenic 35.0%
GAGTTAAAGAGACAAAGAAG+TGG - chr4.3:27490117-27490136 MS.gene006933:intron 35.0%
TATCATGATGTGACTACTTG+CGG - chr4.3:27489137-27489156 MS.gene006933:intron 35.0%
TGGGAATTATGGTTGGAATT+TGG + chr4.3:27488492-27488511 None:intergenic 35.0%
TGGGTATCTTTGGTTAACAA+AGG + chr4.3:27490227-27490246 None:intergenic 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG - chr4.3:27489566-27489585 MS.gene006933:CDS 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG - chr4.3:27489567-27489586 MS.gene006933:CDS 35.0%
! TCAAGGACTAGTAATTGAAC+TGG + chr4.3:27489348-27489367 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG - chr4.3:27490072-27490091 MS.gene006933:intron 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG - chr4.3:27490089-27490108 MS.gene006933:intron 35.0%
AAAGAATGACAGAGGGAAAG+AGG + chr4.3:27490432-27490451 None:intergenic 40.0%
AAGAATGACAGAGGGAAAGA+GGG + chr4.3:27490431-27490450 None:intergenic 40.0%
ACAATAAGGTTGGACTATGG+AGG + chr4.3:27488532-27488551 None:intergenic 40.0%
AGAATGCTAATGAATGCCCT+TGG + chr4.3:27488779-27488798 None:intergenic 40.0%
CAATTGTTTCCTCCATCTCA+AGG + chr4.3:27489521-27489540 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG - chr4.3:27489519-27489538 MS.gene006933:CDS 40.0%
CTTGTTAACCAATGCCCTAA+AGG - chr4.3:27488626-27488645 MS.gene006933:intron 40.0%
GATTCCTTACCAATGCCTTT+AGG + chr4.3:27488644-27488663 None:intergenic 40.0%
GGGTGCAAATACAATAAGGT+TGG + chr4.3:27488542-27488561 None:intergenic 40.0%
GTTAGTATCATAGTCGGGTT+GGG - chr4.3:27488845-27488864 MS.gene006933:intron 40.0%
TGTTAGTATCATAGTCGGGT+TGG - chr4.3:27488844-27488863 MS.gene006933:intron 40.0%
TTACCGAGATGGTGATTGAA+GGG + chr4.3:27488582-27488601 None:intergenic 40.0%
TTGTTCACTGCAGTTTGAAG+TGG + chr4.3:27490147-27490166 None:intergenic 40.0%
! ACTATGGAGGAGGTTTTTGA+AGG + chr4.3:27488519-27488538 None:intergenic 40.0%
! TGTTGGATTTGTTCAGCTGA+TGG + chr4.3:27489399-27489418 None:intergenic 40.0%
! TTGAAGGTGCATGGGAATTA+TGG + chr4.3:27488503-27488522 None:intergenic 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG - chr4.3:27490095-27490114 MS.gene006933:intron 40.0%
!! CTATTGGTTCCAGAATCACA+AGG - chr4.3:27489470-27489489 MS.gene006933:CDS 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG - chr4.3:27490092-27490111 MS.gene006933:intron 40.0%
AAACTGCAGTGAACAAGCAG+AGG - chr4.3:27490151-27490170 MS.gene006933:intron 45.0%
ACACTTTAACCAATGCGCCA+AGG - chr4.3:27488759-27488778 MS.gene006933:intron 45.0%
AGGTGCATGGGAATTATGGT+TGG + chr4.3:27488499-27488518 None:intergenic 45.0%
ATAAGGTTGGACTATGGAGG+AGG + chr4.3:27488529-27488548 None:intergenic 45.0%
ATCCCCTTCAATCACCATCT+CGG - chr4.3:27488576-27488595 MS.gene006933:intron 45.0%
ATCCTCAACCAAGTTCAAGG+TGG - chr4.3:27489313-27489332 MS.gene006933:intron 45.0%
CACTTTAACCAATGCGCCAA+GGG - chr4.3:27488760-27488779 MS.gene006933:intron 45.0%
CTTACCGAGATGGTGATTGA+AGG + chr4.3:27488583-27488602 None:intergenic 45.0%
GAAGCTGCGAAGCTATTTGT+TGG + chr4.3:27489416-27489435 None:intergenic 45.0%
TACCGAGATGGTGATTGAAG+GGG + chr4.3:27488581-27488600 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG - chr4.3:27489594-27489613 MS.gene006933:CDS 45.0%
TGCATCCTCAACCAAGTTCA+AGG - chr4.3:27489310-27489329 MS.gene006933:intron 45.0%
TTAAGCTTGGCTTACCGAGA+TGG + chr4.3:27488593-27488612 None:intergenic 45.0%
! AACCAATGCCCTAAAGGCAT+TGG - chr4.3:27488632-27488651 MS.gene006933:intron 45.0%
!! AGGAGGTTTTTGAAGGTGCA+TGG + chr4.3:27488512-27488531 None:intergenic 45.0%
!! ATGCCCTAAAGGCATTGGTA+AGG - chr4.3:27488637-27488656 MS.gene006933:intron 45.0%
!! GGAGGTTTTTGAAGGTGCAT+GGG + chr4.3:27488511-27488530 None:intergenic 45.0%
!! TACCAATGCCTTTAGGGCAT+TGG + chr4.3:27488637-27488656 None:intergenic 45.0%
AATGAATGCCCTTGGCGCAT+TGG + chr4.3:27488771-27488790 None:intergenic 50.0%
AGGGGATGAGATGGTTGCAA+TGG + chr4.3:27488563-27488582 None:intergenic 50.0%
CTCCACCTTGAACTTGGTTG+AGG + chr4.3:27489318-27489337 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG - chr4.3:27489423-27489442 MS.gene006933:CDS 50.0%
GGGGATGAGATGGTTGCAAT+GGG + chr4.3:27488562-27488581 None:intergenic 50.0%
GGTGATTGAAGGGGATGAGA+TGG + chr4.3:27488572-27488591 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG + chr4.3:27489324-27489343 None:intergenic 50.0%
ACAGCTCATGCAGCTGAGCA+AGG - chr4.3:27489273-27489292 MS.gene006933:intron 55.0%
GCTCTCTCGCCTTGTGATTC+TGG + chr4.3:27489482-27489501 None:intergenic 55.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG - chr4.3:27489506-27489525 MS.gene006933:CDS 60.0%
GCGTCGTCGCCTTGAGATGG+AGG - chr4.3:27489509-27489528 MS.gene006933:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 27488453 27490514 27488453 ID=MS.gene006933
chr4.3 mRNA 27488453 27490514 27488453 ID=MS.gene006933.t1;Parent=MS.gene006933
chr4.3 exon 27490370 27490514 27490370 ID=MS.gene006933.t1.exon1;Parent=MS.gene006933.t1
chr4.3 CDS 27490370 27490514 27490370 ID=cds.MS.gene006933.t1;Parent=MS.gene006933.t1
chr4.3 exon 27489352 27489690 27489352 ID=MS.gene006933.t1.exon2;Parent=MS.gene006933.t1
chr4.3 CDS 27489352 27489690 27489352 ID=cds.MS.gene006933.t1;Parent=MS.gene006933.t1
chr4.3 exon 27488795 27488856 27488795 ID=MS.gene006933.t1.exon3;Parent=MS.gene006933.t1
chr4.3 CDS 27488795 27488856 27488795 ID=cds.MS.gene006933.t1;Parent=MS.gene006933.t1
chr4.3 exon 27488453 27488464 27488453 ID=MS.gene006933.t1.exon4;Parent=MS.gene006933.t1
chr4.3 CDS 27488453 27488464 27488453 ID=cds.MS.gene006933.t1;Parent=MS.gene006933.t1
Gene Sequence

>MS.gene006933

ATGTCTGATTCATTCTGTAGCTTTCTCTCTGCCACTCCAAATTCCAACCATAATTCCCATGCACCTTCAAAAACCTCCTCCATAGTCCAACCTTATTGTATTTGCACCCATTGCAACCATCTCATCCCCTTCAATCACCATCTCGGTAAGCCAAGCTTAATTTTCTTAAGGGTCTTGTTAACCAATGCCCTAAAGGCATTGGTAAGGAATCTATTAATGGAAAGTTAATTTATCTTGAAAATATAAGTTTTTATTTTTGTTTTAGGAATAATTACACCATTTTTTAATGCAAACCTACTAATTTTAACACTTTAACCAATGCGCCAAGGGCATTCATTAGCATTCTACTTTTCTTAAATATCTCTCTTATTTTAACAGAAAATATTAGTATTGTTAGTATCATAGTCGGGTTGGGATCGAACATTTGACATCCTTATCATTCCACTTTTTAACTTTCTCATATGTTTGAATATTTTCTCGAATATCCCATCATAGTATAATCTTCTCTCTTTTTCAATATGAGTAACTATATTCATGTGGTCATTTACTTATTTACTTGTTTGTATATTTCTGCTAAGAAATTTGACCATACATACACTAAAATAATTTGATTTTGAGCTATATATAAACAACGGTTTTAAATTGCAAGAAATTCCAGATAAATACAGTCATATGATATCATAATATCATGATGTGACTACTTGCGGCAGTAATTTAAAAATCATGTTATATATGCCCTAAGTAGCTAAACTATTTTCTTCATGTGTTTTTTTTTTTTTTTTTGAACAATTTTCTTCATGTGTTTTTGTATATATATTATACAGCTCATGCAGCTGAGCAAGGTACAAGCAATACTATGCATCCTCAACCAAGTTCAAGGTGGAGTCCAACACCAGTTCAATTACTAGTCCTTGAAGAATTGTATAGGCAAGGGATGAAAACACCATCAGCTGAACAAATCCAACAAATAGCTTCGCAGCTTCGACAATTTGGAAAGATTGAAGGAAAAAATGTGTTCTATTGGTTCCAGAATCACAAGGCGAGAGAGCGACAGAAGCGTCGTCGCCTTGAGATGGAGGAAACAATTGGTTCTTCTTCAACTGAAGATAAAAAAGAGAAAGAGAAGTACGTTATGGGAAATTCAGAAAAGCAAGAAGCTGCAGGTATACAGCTAGATATTATCTTTAGGGTTATTCAAGGTTCTTTTAGTGGTCAAAAGATTCCTATATTATATTTCACTCATGACATTACAAGTTACAGCAGAGAGAGAGAGAGACAGAAAAAGATAAATAGCTTTTGTAAGGATCTCGGTAAATTTAACAAATTTACAAAATATCAATTTAGCCCATTTGGTCATTTTTTTTTTCTTCAAATATAGCCCTACAATTTTAGAGATTTTAGGTTTATTCTGATGCAACAAGAAACTATAGAAGTCAATATGATATTCTTGCATAGGATAAACCTAACATCTTTAAAAGTATAGTGCCATATTTGAAAGAAAAAAATTTATTGTTGTTTATTCAATTTAATGGACTAAATTATTGCTATTTTTGAAATTTTAGGCATTAAATTAACACCCTTACAATTTGAAGTGTGTGTGAATATGTATATGTACTATATGAGTTGAATTTGTGGTGATTGGATTTTGAAAGAGACAGGTGGTGGAGTTAAAGAGACAAAGAAGTGGGCAACCACTTCAAACTGCAGTGAACAAGCAGAGGTCTGTAATTTCTCTTGTCTTTGTTGATGGTACAATTACAACATTTATTCACCTTTGTTAACCAAAGATACCCAAAAGATTTCATAATCAAAGAGGTTTTGTATGTTGGAAAAAAAGAGTGTCCTCTAAAGCTGTTTTTTTTAACTTTCTGTTGGGGCTGTTTTATGTTACATTTCAGATTTTCTTGGACTTTTTGTGAATTCAATCTGAAAAAAAATGAATTCTCTTCAGACTTCAATCACATATTATCACTCTCCCTCTTTCCCTCTGTCATTCTTTCTATAAAAATAATTAAATAATTTAAATATTATTTTTTACTTTTAACTTTAGTATCTGCTGTAA
Protein sequence

>MS.gene006933.t1

MSDSFCSFLSATPNSNHNSHAPSKTSSIVQPYCICTHCNHLIPFNHHLAHAAEQGTSNTMHPQPSSRWSPTPVQLLVLEELYRQGMKTPSAEQIQQIASQLRQFGKIEGKNVFYWFQNHKARERQKRRRLEMEETIGSSSTEDKKEKEKYVMGNSEKQEAAGGGVKETKKWATTSNCSEQAEYLL