AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007220.t1 MTR_7g104610 98.039 153 3 0 1 153 1 153 1.93e-107 303
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007220.t1 AT5G15770 71.918 146 41 0 7 152 3 148 3.14e-74 219

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCGTTGCAATAATCTTTC+CGG 0.199227 7.3:-9862050 None:intergenic
GTCTGTAATCTCTAATCTTC+TGG 0.252175 7.3:-9861888 None:intergenic
TCGTTCTGTTGGGTGTTATA+AGG 0.300594 7.3:+9862204 MS.gene007220:CDS
TAAGAAATTGTGGTAAAGTT+GGG 0.348212 7.3:+9862098 MS.gene007220:CDS
TTAAGAAATTGTGGTAAAGT+TGG 0.366943 7.3:+9862097 MS.gene007220:CDS
ACCGATCATGCTCGTTCTGT+TGG 0.371791 7.3:+9862193 MS.gene007220:CDS
AGTGCTCGTGGGAAACAATT+GGG 0.376890 7.3:+9862148 MS.gene007220:CDS
ACCGGAAAGATTATTGCAAC+GGG 0.406061 7.3:+9862049 MS.gene007220:CDS
GTTATTGAAGATGAGATTAC+CGG 0.417687 7.3:+9862031 MS.gene007220:CDS
AAGTGCTCGTGGGAAACAAT+TGG 0.427736 7.3:+9862147 MS.gene007220:CDS
CCGATCATGCTCGTTCTGTT+GGG 0.443895 7.3:+9862194 MS.gene007220:CDS
AGTCTCTGATAAGGAATTTG+AGG 0.451854 7.3:+9861961 MS.gene007220:CDS
TTGTGATTCAGTCTCTGATA+AGG 0.468883 7.3:+9861952 MS.gene007220:CDS
TTTCGAGAGGTTGATTCACT+TGG 0.471380 7.3:+9861989 MS.gene007220:CDS
CGATTGCAGTGTTGAGAACA+AGG 0.494534 7.3:+9862234 MS.gene007220:CDS
CTATGAGAAATGTGGTTTCA+AGG 0.496011 7.3:+9862261 MS.gene007220:CDS
CCCAACAGAACGAGCATGAT+CGG 0.532044 7.3:-9862194 None:intergenic
TACCGGAAAGATTATTGCAA+CGG 0.546187 7.3:+9862048 MS.gene007220:CDS
CAAGGAGAAAGAAGTTCAGA+TGG 0.566779 7.3:+9862279 MS.gene007220:CDS
GTTGTGGATTCAAGTGCTCG+TGG 0.610212 7.3:+9862136 MS.gene007220:CDS
ATTTGAGGATCGATTTCGAG+AGG 0.616403 7.3:+9861976 MS.gene007220:CDS
GCATATAGAAGATGTTGTTG+TGG 0.621571 7.3:+9862120 MS.gene007220:CDS
GTGCTCGTGGGAAACAATTG+GGG 0.624260 7.3:+9862149 MS.gene007220:CDS
TTGTGGATTCAAGTGCTCGT+GGG 0.642750 7.3:+9862137 MS.gene007220:CDS
AAGGTGTTCTATGAGAAATG+TGG 0.671486 7.3:+9862253 MS.gene007220:CDS
TTAGAAGATGCAAAACAGTG+AGG 0.719583 7.3:+9861850 None:intergenic
CCGGAAAGATTATTGCAACG+GGG 0.727047 7.3:+9862050 MS.gene007220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAATTTTTAAGAAATTG+TGG + chr7.3:9862088-9862107 MS.gene007220:CDS 15.0%
! TAAGAAATTGTGGTAAAGTT+GGG + chr7.3:9862098-9862117 MS.gene007220:CDS 25.0%
! TTAAGAAATTGTGGTAAAGT+TGG + chr7.3:9862097-9862116 MS.gene007220:CDS 25.0%
GAGATTACAGACAAGAAAAA+AGG + chr7.3:9861899-9861918 MS.gene007220:CDS 30.0%
GTTATTGAAGATGAGATTAC+CGG + chr7.3:9862031-9862050 MS.gene007220:CDS 30.0%
AGTCTCTGATAAGGAATTTG+AGG + chr7.3:9861961-9861980 MS.gene007220:CDS 35.0%
CTATGAGAAATGTGGTTTCA+AGG + chr7.3:9862261-9862280 MS.gene007220:CDS 35.0%
GCATATAGAAGATGTTGTTG+TGG + chr7.3:9862120-9862139 MS.gene007220:CDS 35.0%
GTCTGTAATCTCTAATCTTC+TGG - chr7.3:9861891-9861910 None:intergenic 35.0%
TACCGGAAAGATTATTGCAA+CGG + chr7.3:9862048-9862067 MS.gene007220:CDS 35.0%
TTGTGATTCAGTCTCTGATA+AGG + chr7.3:9861952-9861971 MS.gene007220:CDS 35.0%
! AAGGTGTTCTATGAGAAATG+TGG + chr7.3:9862253-9862272 MS.gene007220:CDS 35.0%
ACCGGAAAGATTATTGCAAC+GGG + chr7.3:9862049-9862068 MS.gene007220:CDS 40.0%
CAAGGAGAAAGAAGTTCAGA+TGG + chr7.3:9862279-9862298 MS.gene007220:CDS 40.0%
! ATTTGAGGATCGATTTCGAG+AGG + chr7.3:9861976-9861995 MS.gene007220:CDS 40.0%
! TCGTTCTGTTGGGTGTTATA+AGG + chr7.3:9862204-9862223 MS.gene007220:CDS 40.0%
! TTTCGAGAGGTTGATTCACT+TGG + chr7.3:9861989-9862008 MS.gene007220:CDS 40.0%
AAGTGCTCGTGGGAAACAAT+TGG + chr7.3:9862147-9862166 MS.gene007220:CDS 45.0%
AGTGCTCGTGGGAAACAATT+GGG + chr7.3:9862148-9862167 MS.gene007220:CDS 45.0%
CCCCGTTGCAATAATCTTTC+CGG - chr7.3:9862053-9862072 None:intergenic 45.0%
CCGGAAAGATTATTGCAACG+GGG + chr7.3:9862050-9862069 MS.gene007220:CDS 45.0%
TTGTGGATTCAAGTGCTCGT+GGG + chr7.3:9862137-9862156 MS.gene007220:CDS 45.0%
! CGATTGCAGTGTTGAGAACA+AGG + chr7.3:9862234-9862253 MS.gene007220:CDS 45.0%
ACCGATCATGCTCGTTCTGT+TGG + chr7.3:9862193-9862212 MS.gene007220:CDS 50.0%
CCCAACAGAACGAGCATGAT+CGG - chr7.3:9862197-9862216 None:intergenic 50.0%
CCGATCATGCTCGTTCTGTT+GGG + chr7.3:9862194-9862213 MS.gene007220:CDS 50.0%
GTGCTCGTGGGAAACAATTG+GGG + chr7.3:9862149-9862168 MS.gene007220:CDS 50.0%
GTTGTGGATTCAAGTGCTCG+TGG + chr7.3:9862136-9862155 MS.gene007220:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 9861857 9862318 9861857 ID=MS.gene007220
chr7.3 mRNA 9861857 9862318 9861857 ID=MS.gene007220.t1;Parent=MS.gene007220
chr7.3 exon 9861857 9862318 9861857 ID=MS.gene007220.t1.exon1;Parent=MS.gene007220.t1
chr7.3 CDS 9861857 9862318 9861857 ID=cds.MS.gene007220.t1;Parent=MS.gene007220.t1
Gene Sequence

>MS.gene007220

ATGCAAAACAGTGAGGAACAAAAGTATAGAGCCAGAAGATTAGAGATTACAGACAAGAAAAAAGGTTTCATAGAATTACTACAACAGCTTAGCGTTTGTGATTCAGTCTCTGATAAGGAATTTGAGGATCGATTTCGAGAGGTTGATTCACTTGGAGATGATCATGTTATATGTGTTATTGAAGATGAGATTACCGGAAAGATTATTGCAACGGGGAGTGTGTTTATTGAGAAGAAATTTTTAAGAAATTGTGGTAAAGTTGGGCATATAGAAGATGTTGTTGTGGATTCAAGTGCTCGTGGGAAACAATTGGGGAAGAAAGTAATTAACTTTCTTACCGATCATGCTCGTTCTGTTGGGTGTTATAAGGTAATTCTCGATTGCAGTGTTGAGAACAAGGTGTTCTATGAGAAATGTGGTTTCAAGGAGAAAGAAGTTCAGATGGCTATGTATTTTGTTTGA
Protein sequence

>MS.gene007220.t1

MQNSEEQKYRARRLEITDKKKGFIELLQQLSVCDSVSDKEFEDRFREVDSLGDDHVICVIEDEITGKIIATGSVFIEKKFLRNCGKVGHIEDVVVDSSARGKQLGKKVINFLTDHARSVGCYKVILDCSVENKVFYEKCGFKEKEVQMAMYFV