AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007222

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007222.t1 MTR_7g104610 98.039 153 3 0 1 153 1 153 1.93e-107 303
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007222.t1 AT5G15770 71.918 146 41 0 7 152 3 148 3.14e-74 219

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCGTTGCAATAATCTTTC+CGG 0.199227 7.3:-9851878 None:intergenic
GTCTGTAATCTCTAATCTTC+TGG 0.252175 7.3:-9851716 None:intergenic
TCGTTCTGTTGGGTGTTATA+AGG 0.300594 7.3:+9852032 MS.gene007222:CDS
TAAGAAATTGTGGTAAAGTT+GGG 0.348212 7.3:+9851926 MS.gene007222:CDS
TTAAGAAATTGTGGTAAAGT+TGG 0.366943 7.3:+9851925 MS.gene007222:CDS
ACCGATCATGCTCGTTCTGT+TGG 0.371791 7.3:+9852021 MS.gene007222:CDS
AGTGCTCGTGGGAAACAATT+GGG 0.376890 7.3:+9851976 MS.gene007222:CDS
ACCGGAAAGATTATTGCAAC+GGG 0.406061 7.3:+9851877 MS.gene007222:CDS
GTTATTGAAGATGAGATTAC+CGG 0.417687 7.3:+9851859 MS.gene007222:CDS
AAGTGCTCGTGGGAAACAAT+TGG 0.427736 7.3:+9851975 MS.gene007222:CDS
CCGATCATGCTCGTTCTGTT+GGG 0.443895 7.3:+9852022 MS.gene007222:CDS
AGTCTCTGATAAGGAATTTG+AGG 0.451854 7.3:+9851789 MS.gene007222:CDS
TTGTGATTCAGTCTCTGATA+AGG 0.468883 7.3:+9851780 MS.gene007222:CDS
TTTCGAGAGGTTGATTCACT+TGG 0.471380 7.3:+9851817 MS.gene007222:CDS
CGATTGCAGTGTTGAGAACA+AGG 0.494534 7.3:+9852062 MS.gene007222:CDS
CTATGAGAAATGTGGTTTCA+AGG 0.496011 7.3:+9852089 MS.gene007222:CDS
CCCAACAGAACGAGCATGAT+CGG 0.532044 7.3:-9852022 None:intergenic
TACCGGAAAGATTATTGCAA+CGG 0.546187 7.3:+9851876 MS.gene007222:CDS
CAAGGAGAAAGAAGTTCAGA+TGG 0.566779 7.3:+9852107 MS.gene007222:CDS
GTTGTGGATTCAAGTGCTCG+TGG 0.610212 7.3:+9851964 MS.gene007222:CDS
ATTTGAGGATCGATTTCGAG+AGG 0.616403 7.3:+9851804 MS.gene007222:CDS
GCATATAGAAGATGTTGTTG+TGG 0.621571 7.3:+9851948 MS.gene007222:CDS
GTGCTCGTGGGAAACAATTG+GGG 0.624260 7.3:+9851977 MS.gene007222:CDS
TTGTGGATTCAAGTGCTCGT+GGG 0.642750 7.3:+9851965 MS.gene007222:CDS
AAGGTGTTCTATGAGAAATG+TGG 0.671486 7.3:+9852081 MS.gene007222:CDS
TTAGAAGATGCAAAACAGTG+AGG 0.719583 7.3:+9851678 None:intergenic
CCGGAAAGATTATTGCAACG+GGG 0.727047 7.3:+9851878 MS.gene007222:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAATTTTTAAGAAATTG+TGG + chr7.3:9851916-9851935 MS.gene007222:CDS 15.0%
! TAAGAAATTGTGGTAAAGTT+GGG + chr7.3:9851926-9851945 MS.gene007222:CDS 25.0%
! TTAAGAAATTGTGGTAAAGT+TGG + chr7.3:9851925-9851944 MS.gene007222:CDS 25.0%
GAGATTACAGACAAGAAAAA+AGG + chr7.3:9851727-9851746 MS.gene007222:CDS 30.0%
GTTATTGAAGATGAGATTAC+CGG + chr7.3:9851859-9851878 MS.gene007222:CDS 30.0%
AGTCTCTGATAAGGAATTTG+AGG + chr7.3:9851789-9851808 MS.gene007222:CDS 35.0%
CTATGAGAAATGTGGTTTCA+AGG + chr7.3:9852089-9852108 MS.gene007222:CDS 35.0%
GCATATAGAAGATGTTGTTG+TGG + chr7.3:9851948-9851967 MS.gene007222:CDS 35.0%
GTCTGTAATCTCTAATCTTC+TGG - chr7.3:9851719-9851738 None:intergenic 35.0%
TACCGGAAAGATTATTGCAA+CGG + chr7.3:9851876-9851895 MS.gene007222:CDS 35.0%
TTGTGATTCAGTCTCTGATA+AGG + chr7.3:9851780-9851799 MS.gene007222:CDS 35.0%
! AAGGTGTTCTATGAGAAATG+TGG + chr7.3:9852081-9852100 MS.gene007222:CDS 35.0%
ACCGGAAAGATTATTGCAAC+GGG + chr7.3:9851877-9851896 MS.gene007222:CDS 40.0%
CAAGGAGAAAGAAGTTCAGA+TGG + chr7.3:9852107-9852126 MS.gene007222:CDS 40.0%
! ATTTGAGGATCGATTTCGAG+AGG + chr7.3:9851804-9851823 MS.gene007222:CDS 40.0%
! TCGTTCTGTTGGGTGTTATA+AGG + chr7.3:9852032-9852051 MS.gene007222:CDS 40.0%
! TTTCGAGAGGTTGATTCACT+TGG + chr7.3:9851817-9851836 MS.gene007222:CDS 40.0%
AAGTGCTCGTGGGAAACAAT+TGG + chr7.3:9851975-9851994 MS.gene007222:CDS 45.0%
AGTGCTCGTGGGAAACAATT+GGG + chr7.3:9851976-9851995 MS.gene007222:CDS 45.0%
CCCCGTTGCAATAATCTTTC+CGG - chr7.3:9851881-9851900 None:intergenic 45.0%
CCGGAAAGATTATTGCAACG+GGG + chr7.3:9851878-9851897 MS.gene007222:CDS 45.0%
TTGTGGATTCAAGTGCTCGT+GGG + chr7.3:9851965-9851984 MS.gene007222:CDS 45.0%
! CGATTGCAGTGTTGAGAACA+AGG + chr7.3:9852062-9852081 MS.gene007222:CDS 45.0%
ACCGATCATGCTCGTTCTGT+TGG + chr7.3:9852021-9852040 MS.gene007222:CDS 50.0%
CCCAACAGAACGAGCATGAT+CGG - chr7.3:9852025-9852044 None:intergenic 50.0%
CCGATCATGCTCGTTCTGTT+GGG + chr7.3:9852022-9852041 MS.gene007222:CDS 50.0%
GTGCTCGTGGGAAACAATTG+GGG + chr7.3:9851977-9851996 MS.gene007222:CDS 50.0%
GTTGTGGATTCAAGTGCTCG+TGG + chr7.3:9851964-9851983 MS.gene007222:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 9851685 9852146 9851685 ID=MS.gene007222
chr7.3 mRNA 9851685 9852146 9851685 ID=MS.gene007222.t1;Parent=MS.gene007222
chr7.3 exon 9851685 9852146 9851685 ID=MS.gene007222.t1.exon1;Parent=MS.gene007222.t1
chr7.3 CDS 9851685 9852146 9851685 ID=cds.MS.gene007222.t1;Parent=MS.gene007222.t1
Gene Sequence

>MS.gene007222

ATGCAAAACAGTGAGGAACAAAAGTATAGAGCCAGAAGATTAGAGATTACAGACAAGAAAAAAGGTTTCATAGAATTACTACAACAGCTTAGCGTTTGTGATTCAGTCTCTGATAAGGAATTTGAGGATCGATTTCGAGAGGTTGATTCACTTGGAGATGATCATGTTATATGTGTTATTGAAGATGAGATTACCGGAAAGATTATTGCAACGGGGAGTGTGTTTATTGAGAAGAAATTTTTAAGAAATTGTGGTAAAGTTGGGCATATAGAAGATGTTGTTGTGGATTCAAGTGCTCGTGGGAAACAATTGGGGAAGAAAGTAATTAACTTTCTTACCGATCATGCTCGTTCTGTTGGGTGTTATAAGGTAATTCTCGATTGCAGTGTTGAGAACAAGGTGTTCTATGAGAAATGTGGTTTCAAGGAGAAAGAAGTTCAGATGGCTATGTATTTTGTTTGA
Protein sequence

>MS.gene007222.t1

MQNSEEQKYRARRLEITDKKKGFIELLQQLSVCDSVSDKEFEDRFREVDSLGDDHVICVIEDEITGKIIATGSVFIEKKFLRNCGKVGHIEDVVVDSSARGKQLGKKVINFLTDHARSVGCYKVILDCSVENKVFYEKCGFKEKEVQMAMYFV